FastQCFastQC Report
Thu 26 May 2016
SRR936573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936573_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260888
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100280.4435425372685423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45250.20014259883727104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.17997353252350404No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36200.1601140790698168No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT35280.15604488148019716No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28410.12565859078379824No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26700.1180951909161356No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24620.10889526593090856No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24280.1073914320390926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG69700.020.8535122
GTACATG72250.020.453011
GAGTACT63750.020.37761112-13
ACATGGG71750.020.1244053
AGAGTAC102700.019.2168210-11
GTACTTT67700.019.18845414-15
AGTACTT68250.018.61646812-13
TATACCG1600.001192084617.8111955
GTGTAGC14100.017.523421
ACCGTAT2253.54159E-516.8880258
TACTTTT74800.016.60509914-15
GTAGGAC6150.016.2186813
ACTTTTT80600.016.20538716-17
CATGGGG47600.016.1651384
GTATAGG7450.015.9447771
CGTATGG1800.002613989615.8384771
GGACCGT3302.2785207E-715.8325246
GTACCGT2709.489868E-615.8325226
GTGTAGG9350.015.7537791
GTATAGC8200.015.6453261
Produced by FastQC (version 0.11.5)