##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936573_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260888 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.897257183902962 31.0 31.0 34.0 30.0 34.0 2 32.05401019422457 31.0 31.0 34.0 30.0 34.0 3 32.168121552239654 33.0 31.0 34.0 30.0 34.0 4 35.894211035663865 37.0 35.0 37.0 35.0 37.0 5 35.66565968769793 37.0 35.0 37.0 33.0 37.0 6 35.76438770960791 37.0 35.0 37.0 33.0 37.0 7 35.70827966710425 37.0 35.0 37.0 33.0 37.0 8 35.67719099751956 37.0 35.0 37.0 33.0 37.0 9 37.327699558757445 39.0 37.0 39.0 34.0 39.0 10-11 37.19149157322256 39.0 37.0 39.0 33.0 39.0 12-13 37.099346141869916 39.0 37.0 39.0 33.0 39.0 14-15 38.22594661920449 40.0 38.0 41.0 33.0 41.0 16-17 38.13791572161027 40.0 37.0 41.0 33.0 41.0 18-19 38.2600557833913 40.0 38.0 41.0 33.5 41.0 20-21 38.27487894137171 40.0 38.0 41.0 34.0 41.0 22-23 38.18257074211549 40.0 37.5 41.0 33.0 41.0 24-25 38.056754248773046 40.0 37.0 41.0 33.0 41.0 26-27 37.84481849609534 40.0 37.0 41.0 33.0 41.0 28-29 37.78429426844673 40.0 37.0 41.0 32.5 41.0 30-31 37.815471620000636 40.0 37.0 41.0 33.0 41.0 32-33 37.70479917625287 40.0 37.0 41.0 33.0 41.0 34-35 37.55963873486878 40.0 37.0 41.0 32.0 41.0 36-37 37.33153300826932 39.5 36.0 41.0 31.5 41.0 38-39 37.18253204050798 39.0 36.0 41.0 31.0 41.0 40-41 36.993185420949644 39.0 36.0 41.0 31.0 41.0 42-43 36.78743993510514 39.0 35.5 41.0 30.5 41.0 44-45 36.58578377168617 39.0 35.0 40.0 30.0 41.0 46-47 36.310005847260015 39.0 35.0 40.0 30.0 41.0 48-49 36.008131318313865 39.0 35.0 40.0 29.0 41.0 50-51 35.710745733534786 38.0 35.0 40.0 28.5 41.0 52-53 35.4493316342959 38.0 34.0 40.0 28.0 41.0 54-55 35.182166255028996 38.0 34.0 40.0 27.0 41.0 56-57 34.845900814193364 38.0 34.0 40.0 26.5 41.0 58-59 34.49727607028743 37.5 33.5 40.0 26.0 41.0 60-61 34.11359076610606 37.0 33.0 39.5 26.0 41.0 62-63 33.706318269635645 36.0 32.5 39.0 25.0 40.0 64-65 33.27860623790298 36.0 32.0 39.0 23.5 40.0 66-67 32.79839204772638 35.0 31.5 38.5 22.5 40.0 68-69 32.48379243023095 35.0 31.0 38.0 22.0 40.0 70-71 32.02272536277781 35.0 31.0 37.5 21.5 39.5 72-73 31.550720336434182 34.5 30.5 37.0 20.5 39.0 74-75 31.01355462986225 34.0 30.0 36.0 19.0 39.0 76-77 29.608175858335308 32.5 28.5 35.0 18.0 37.0 78-79 30.40542189617531 34.0 30.0 35.0 18.0 37.0 80-81 30.212717525149408 34.0 30.0 35.0 17.5 37.0 82-83 29.860810663774586 34.0 29.5 35.0 14.5 36.5 84-85 29.43089662115063 34.0 29.0 35.0 9.0 36.0 86-87 29.06080310037472 34.0 29.0 35.0 7.0 36.0 88-89 28.64604880914048 33.0 29.0 35.0 2.0 35.0 90-91 28.149746028993917 33.0 27.5 35.0 2.0 35.0 92-93 27.632721523578347 33.0 27.0 34.5 2.0 35.0 94-95 27.008012559666824 32.0 25.5 34.0 2.0 35.0 96-97 26.203510965602895 32.0 25.0 34.0 2.0 35.0 98-99 25.20548585334612 32.0 21.5 34.0 2.0 35.0 100-101 21.951179138462408 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25807758169553807 1101 2 0.3497568336878629 1101 3 0.31294858902688816 1101 4 0.17034495350661416 1101 5 0.11686701914182152 1101 6 0.13118022362706938 1101 7 0.15116482106530782 1101 8 0.10122011970130984 1101 9 0.1963626256565547 1101 10-11 0.24877192341440946 1101 12-13 0.2845192333104407 1101 14-15 0.37584889741319927 1101 16-17 0.3499035682398812 1101 18-19 0.33334146575087686 1101 20-21 0.22924516621025504 1101 22-23 0.2900337633008476 1101 24-25 0.17956608199860113 1101 26-27 0.20317024727005162 1101 28-29 0.2119494425524664 1101 30-31 0.29925481026234735 1101 32-33 0.3283841981274591 1101 34-35 0.1359253631800641 1101 36-37 0.2053926993622568 1101 38-39 0.28052412691297235 1101 40-41 0.26660624867940186 1101 42-43 0.2794378469751777 1101 44-45 0.2959397538236388 1101 46-47 0.31323473105279476 1101 48-49 0.3398102492389441 1101 50-51 0.37656490790770647 1101 52-53 0.34512899322842117 1101 54-55 0.4880492301008772 1101 56-57 0.3825934825589741 1101 58-59 0.3197136463088128 1101 60-61 0.2858894626181723 1101 62-63 0.36605291995994804 1101 64-65 0.39158659423432596 1101 66-67 0.17367588233678077 1101 68-69 0.12120094996228659 1101 70-71 0.26253593172197043 1101 72-73 0.22483395526517924 1101 74-75 0.17775364404090865 1101 76-77 0.11192772918802518 1101 78-79 -0.08658010126261928 1101 80-81 0.18442089996324285 1101 82-83 0.38734237314119113 1101 84-85 0.30641339323058503 1101 86-87 0.20373226106942965 1101 88-89 0.10577304538908194 1101 90-91 -0.04812279981887713 1101 92-93 -0.026721401774359776 1101 94-95 0.06040207167841061 1101 96-97 0.24709760036968476 1101 98-99 0.42703208002647486 1101 100-101 0.9138094006995061 1102 1 0.21758294718335947 1102 2 0.273326570793337 1102 3 0.1974925844895168 1102 4 0.08406949238842287 1102 5 0.07938761538875383 1102 6 0.1383074433024305 1102 7 0.12678273694577769 1102 8 0.12048001294677135 1102 9 0.3110333604645916 1102 10-11 0.2595300902338309 1102 12-13 0.21670159697232094 1102 14-15 0.2868127490957306 1102 16-17 0.31800846296447816 1102 18-19 0.2294962350686447 1102 20-21 0.18076059727293625 1102 22-23 0.1670201782484142 1102 24-25 0.24808584855880156 1102 26-27 0.013400478218507317 1102 28-29 0.03918753958025434 1102 30-31 0.14781093042626736 1102 32-33 0.20947776167750476 1102 34-35 0.20831880438405648 1102 36-37 0.20716994869166427 1102 38-39 0.2517833402366989 1102 40-41 0.29102819420651116 1102 42-43 0.21575056427322892 1102 44-45 0.41924959455169386 1102 46-47 0.30962698894244056 1102 48-49 0.22499373556890134 1102 50-51 0.11974725477085713 1102 52-53 0.15543809281901844 1102 54-55 0.2549455554200719 1102 56-57 0.28744900061633416 1102 58-59 0.26112406818771206 1102 60-61 0.38222722426915823 1102 62-63 0.10675446364989938 1102 64-65 0.1478753719098833 1102 66-67 0.20974063320725378 1102 68-69 0.1618087623995521 1102 70-71 0.16367821730105447 1102 72-73 0.07490452419281368 1102 74-75 0.06746780642887984 1102 76-77 -0.11519189404194918 1102 78-79 -0.10012372629888233 1102 80-81 0.13216017446854522 1102 82-83 0.13916952041115493 1102 84-85 0.1401088959242216 1102 86-87 0.11285109902967605 1102 88-89 -0.019982292694020742 1102 90-91 0.15844257331664124 1102 92-93 0.11046832343533097 1102 94-95 0.31315115636083846 1102 96-97 0.5037842803101995 1102 98-99 0.4634538752191304 1102 100-101 0.7746868243086773 1103 1 0.06255630940302837 1103 2 0.025686694660301157 1103 3 0.0822918079556132 1103 4 -0.0027590699694002296 1103 5 -0.18712954684264815 1103 6 -0.01960326783824229 1103 7 -0.047398194769506574 1103 8 -0.012695494316709244 1103 9 -0.013915071049780181 1103 10-11 -0.043836096456907114 1103 12-13 -0.06020236167484683 1103 14-15 -0.08021151986861241 1103 16-17 0.1026090786514473 1103 18-19 -0.03784924329140438 1103 20-21 -0.03711889063874452 1103 22-23 -0.09820299830190038 1103 24-25 -0.12277378199480893 1103 26-27 -0.05328744851463085 1103 28-29 0.024600882580365635 1103 30-31 -0.07237681362988724 1103 32-33 -0.12003132457851962 1103 34-35 -0.04048068583033171 1103 36-37 -0.017841493555913246 1103 38-39 0.14363158668020048 1103 40-41 0.11660630557164353 1103 42-43 0.024348836223104797 1103 44-45 0.06625611449540258 1103 46-47 -0.10353974640621288 1103 48-49 -0.061198677722224204 1103 50-51 0.06558837538725015 1103 52-53 -0.03544210813136317 1103 54-55 -0.11630308305221604 1103 56-57 -0.12835130409229123 1103 58-59 0.05207563000463722 1103 60-61 -0.028047173082050847 1103 62-63 -0.004234340331713327 1103 64-65 -0.07365608814370717 1103 66-67 0.06622948478177904 1103 68-69 0.050682024474618004 1103 70-71 0.019529836856442273 1103 72-73 -0.17962093459428985 1103 74-75 -0.17177762080440928 1103 76-77 -0.19784567471444348 1103 78-79 -0.31862291949931176 1103 80-81 -0.06187939775384166 1103 82-83 0.01937802295580937 1103 84-85 -0.24500766110775984 1103 86-87 -0.31286164042393594 1103 88-89 -0.43009025735329587 1103 90-91 -0.5417470288579267 1103 92-93 -0.4539539527825198 1103 94-95 -0.08713870441137672 1103 96-97 -0.14115950020839563 1103 98-99 -0.0899710188937668 1103 100-101 0.026074570872726355 1104 1 0.11663674857931383 1104 2 0.20030113227316804 1104 3 0.07712377595875353 1104 4 0.029998802409771486 1104 5 0.012360627392169476 1104 6 0.06938503963719 1104 7 0.09327693706261186 1104 8 0.02490105149842492 1104 9 0.020733213033508946 1104 10-11 0.033950767753935907 1104 12-13 0.0360809127050743 1104 14-15 0.036995188402485724 1104 16-17 0.09032761130879408 1104 18-19 -0.011759846981398425 1104 20-21 -0.10147360212183543 1104 22-23 0.02855658650526749 1104 24-25 0.0014780655470616466 1104 26-27 0.12241883933646847 1104 28-29 0.08112076294692372 1104 30-31 -0.04350472183050158 1104 32-33 -0.04999976055700728 1104 34-35 0.024224966955969762 1104 36-37 0.19824433125371144 1104 38-39 0.209673004258363 1104 40-41 0.17483003996554203 1104 42-43 0.052702055244779444 1104 44-45 0.022210763536364198 1104 46-47 0.03001396742199347 1104 48-49 0.049447468191296196 1104 50-51 -0.017588261323751908 1104 52-53 0.1859205493487437 1104 54-55 0.049523334933951446 1104 56-57 0.10936959379709776 1104 58-59 0.14429040963004525 1104 60-61 0.18557881041099478 1104 62-63 0.11971564447167538 1104 64-65 0.1990761569463686 1104 66-67 0.004574390012841434 1104 68-69 0.22386194885815058 1104 70-71 -0.007524574123909389 1104 72-73 -0.03597329740196287 1104 74-75 0.1861612213908579 1104 76-77 0.05818389305122906 1104 78-79 0.10818940998224491 1104 80-81 0.26003784750771075 1104 82-83 0.16792346438497674 1104 84-85 0.22454441124941837 1104 86-87 0.12746355532857834 1104 88-89 0.16562477003018117 1104 90-91 0.18093217590375943 1104 92-93 0.2953607212352871 1104 94-95 0.220155198138702 1104 96-97 0.45516398311612605 1104 98-99 0.47089822753147814 1104 100-101 1.0127929060332015 1105 1 -0.04656977449268496 1105 2 0.05551679749029148 1105 3 0.013713482182154735 1105 4 -0.05105232915011726 1105 5 -0.12618774728261428 1105 6 -0.11180158050986222 1105 7 -0.040863433244176406 1105 8 -0.10644328011774462 1105 9 -0.1698541379516385 1105 10-11 -0.1170708179143034 1105 12-13 -0.3766253266110553 1105 14-15 -0.13382735080595154 1105 16-17 0.017699339242206236 1105 18-19 -0.1034312787674807 1105 20-21 -0.07700627363063717 1105 22-23 -0.15359381400140393 1105 24-25 -0.08070959539673339 1105 26-27 -0.014545751631267478 1105 28-29 -0.18153767458439773 1105 30-31 -0.06411316411619339 1105 32-33 -0.052653275924356535 1105 34-35 -0.105372512758386 1105 36-37 -0.2644573688290208 1105 38-39 0.04872340523832008 1105 40-41 0.009046372514511347 1105 42-43 -0.1727259557405958 1105 44-45 0.03141379032300051 1105 46-47 0.05205592542785098 1105 48-49 -0.0816332221680156 1105 50-51 -0.016026635959804025 1105 52-53 0.1292812428575516 1105 54-55 0.15733948608569648 1105 56-57 -0.004468140871587423 1105 58-59 0.12518604555513946 1105 60-61 0.030729424756430035 1105 62-63 0.03312051698375029 1105 64-65 -0.025010628893454623 1105 66-67 0.09437451745558434 1105 68-69 0.1692037703427971 1105 70-71 0.07738791763124908 1105 72-73 0.09104585472087123 1105 74-75 -0.3042104407129109 1105 76-77 -0.22664310622292305 1105 78-79 -0.1195908274433286 1105 80-81 0.0200002815866398 1105 82-83 -0.10933742880707698 1105 84-85 -0.2617332280566451 1105 86-87 -0.24841368917173767 1105 88-89 -0.09181462475017454 1105 90-91 -0.002243854706591719 1105 92-93 0.08243162922395086 1105 94-95 0.026130184596294015 1105 96-97 0.05328446082418736 1105 98-99 0.20367226707486807 1105 100-101 0.3802411598482003 1106 1 0.1686572095627099 1106 2 0.1907248186493149 1106 3 0.14960294305990374 1106 4 0.09884714935763128 1106 5 0.07624570683441334 1106 6 0.05448936414020267 1106 7 0.14234520674123274 1106 8 0.021084213688325804 1106 9 0.15032615318400389 1106 10-11 0.1756775245960185 1106 12-13 0.1990374739865075 1106 14-15 0.26933879273909156 1106 16-17 0.21871964687652223 1106 18-19 0.1005671308285585 1106 20-21 0.11431041555751875 1106 22-23 0.18630123747885108 1106 24-25 -0.009482893038367024 1106 26-27 0.05139371424798611 1106 28-29 0.031996040744886045 1106 30-31 0.12279350522466359 1106 32-33 0.136372672201567 1106 34-35 0.12529212340521667 1106 36-37 0.20807156495781243 1106 38-39 0.2809802721072572 1106 40-41 0.2904113079875046 1106 42-43 0.4041438838558733 1106 44-45 0.24744542505014522 1106 46-47 0.035606003373260364 1106 48-49 0.12662346127029167 1106 50-51 0.07591569526701392 1106 52-53 0.02058782824362737 1106 54-55 0.1194326537233934 1106 56-57 0.16659111805358862 1106 58-59 0.06265850261586792 1106 60-61 0.021717450617131817 1106 62-63 0.03435325642686138 1106 64-65 0.21354322474957144 1106 66-67 0.33950781079365555 1106 68-69 0.32632513665903673 1106 70-71 0.2480217964103133 1106 72-73 0.2231938852479587 1106 74-75 0.12679552002638061 1106 76-77 -0.09528996271844647 1106 78-79 0.09533908971305394 1106 80-81 0.1721011165235211 1106 82-83 0.44565100221356246 1106 84-85 0.2470415209041903 1106 86-87 0.2599322781136131 1106 88-89 0.29802697232582176 1106 90-91 0.4831044354453766 1106 92-93 0.36641221480605424 1106 94-95 0.6173437066582359 1106 96-97 0.5256191294887671 1106 98-99 0.8714064680417621 1106 100-101 1.2180295308003473 1107 1 -0.01933472027789307 1107 2 0.09611685237064194 1107 3 0.03802728572983938 1107 4 0.004344969616361993 1107 5 -0.004431581113642835 1107 6 0.07766476388142252 1107 7 -0.05513257152604467 1107 8 0.0040626626505329 1107 9 0.007283834564070446 1107 10-11 -0.0013594221117401162 1107 12-13 -0.008076339208002992 1107 14-15 0.023231454205458135 1107 16-17 0.04915390889872384 1107 18-19 0.017100862302278586 1107 20-21 -0.040763740667102866 1107 22-23 -0.004911450497679937 1107 24-25 0.016115239373512225 1107 26-27 0.02323895876335058 1107 28-29 -0.0865535871585621 1107 30-31 -0.18071946405291328 1107 32-33 -0.09002564007504787 1107 34-35 -0.19619440669553967 1107 36-37 -0.07330860962311903 1107 38-39 0.060434745020302216 1107 40-41 -0.06522825380212538 1107 42-43 -0.17997312378823693 1107 44-45 -0.13451337150784326 1107 46-47 -0.12325319792180522 1107 48-49 -0.12686718373342387 1107 50-51 -0.10720287252136984 1107 52-53 -0.16333815598202506 1107 54-55 0.006909267786348039 1107 56-57 -0.2075100588975971 1107 58-59 -0.2803734030733622 1107 60-61 -0.3601406691970368 1107 62-63 -0.23439081293265218 1107 64-65 -0.2923146702336794 1107 66-67 -0.28611763829850645 1107 68-69 -0.14471324382918027 1107 70-71 -0.13998430274978446 1107 72-73 -0.20817585568688912 1107 74-75 -0.10502904777829869 1107 76-77 -0.2303275134866638 1107 78-79 -0.20030286401237873 1107 80-81 0.16758212556136698 1107 82-83 0.11181927986924833 1107 84-85 -0.0351187258132164 1107 86-87 0.0989283445392708 1107 88-89 0.08107929233092293 1107 90-91 0.06502664864336083 1107 92-93 -0.11968334634388 1107 94-95 0.12316106854914821 1107 96-97 0.3692936952978698 1107 98-99 0.6000946583558857 1107 100-101 0.9954815860905235 1108 1 0.06086267510055876 1108 2 0.10694775412265045 1108 3 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-0.7218739282765299 2316 72-73 -0.3376328744454149 2316 74-75 -0.49754842395506316 2316 76-77 -0.4283393384336165 2316 78-79 -0.5259354538451149 2316 80-81 -1.1759767899704556 2316 82-83 -0.6227532487965348 2316 84-85 -0.6744742811473934 2316 86-87 -0.9110356924796896 2316 88-89 -0.5465523062783753 2316 90-91 -0.7147249975845114 2316 92-93 -0.49274535509055895 2316 94-95 -0.3482259469219038 2316 96-97 -0.2803411957671713 2316 98-99 -0.7458851585417356 2316 100-101 -1.0299650870846797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 25.0 5 57.0 6 97.0 7 287.0 8 790.0 9 1390.0 10 2308.0 11 3492.0 12 5008.0 13 6748.0 14 8930.0 15 11312.0 16 14076.0 17 17318.0 18 16462.0 19 13224.0 20 13074.0 21 13829.0 22 14758.0 23 16948.0 24 20122.0 25 24152.0 26 29794.0 27 37296.0 28 45924.0 29 57013.0 30 70665.0 31 87283.0 32 108817.0 33 139023.0 34 181985.0 35 245763.0 36 348647.0 37 443787.0 38 248294.0 39 12179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01823695699402 18.895738692551284 14.69978288388779 23.386241466566908 2 16.10147825086917 20.259644901317245 38.840886773591414 24.79799007422217 3 20.598875709205977 22.882046777184918 29.18042836991989 27.33864914368921 4 13.750699950550096 16.45730810297257 36.00753513906569 33.784456807411644 5 14.45502986243773 37.03384586532511 33.820078757397056 14.691045514840104 6 33.50742476052477 35.444589962939226 16.458451373601708 14.5895339029343 7 29.779316799416865 31.886630385936854 19.609463184377113 18.72458963026917 8 26.74059926895981 34.74904550778278 18.881607580738187 19.628747642519222 9 25.97261631211477 17.167550926980518 19.811618273779377 37.04821448712533 10-11 25.907014083011926 26.041209617494072 26.511690138353206 21.540086161140795 12-13 26.657127534292773 23.19095035633525 27.00717093587071 23.144751173501263 14-15 23.11122119838599 25.081690041168464 25.4339938938422 26.373094866603342 16-17 22.864303993096293 28.117388666783587 26.108709936175657 22.909597403944463 18-19 22.749026046403007 27.30907059527053 27.843020087682362 22.0988832706441 20-21 23.610661742346757 26.00582735761952 27.873943307661257 22.509567592372466 22-23 23.125897061067604 25.98828761685292 27.885246953033256 23.000568369046224 24-25 23.480607022960186 26.00828442148376 27.949638855838682 22.561469699717364 26-27 23.12198211734857 26.043831051429045 28.255641987805614 22.578544843416772 28-29 22.782665121176553 26.897211858872073 27.85323505714753 22.466887962803845 30-31 23.23558090279183 26.14468934339055 27.622354192389682 22.99737556142794 32-33 23.067957875666472 26.19371186908098 27.843822655662386 22.89450759959016 34-35 23.58441366571063 26.171991804395407 27.693672469527463 22.5499220603665 36-37 23.515968151503966 26.026694646937447 27.92093656642885 22.536400635129734 38-39 23.12832790547228 26.45486765787335 27.922458074611434 22.49434636204294 40-41 23.368854516483236 26.34591078827392 27.56040573101135 22.72482896423149 42-43 23.13066662774376 26.396585930386824 27.247936035511845 23.22481140635757 44-45 23.41017381717737 26.11753752179764 27.36567290435798 23.106615756667008 46-47 23.7807993057592 26.67216450888523 27.0594240225186 22.487612162836975 48-49 23.759611601616985 26.556140113040332 26.94862098714458 22.735627298198107 50-51 23.21960171412383 26.82994793803341 27.21307588892474 22.73737445891802 52-53 23.687566319992886 27.05484163040031 26.489205902816632 22.768386146790167 54-55 23.400190198159944 26.667352264685068 26.711783439490443 23.220674097664542 56-57 23.613720131295576 26.71544766510693 26.673096393263958 22.997735810333534 58-59 24.022023409603484 26.559302249977552 26.601940759894322 22.81673358052464 60-61 23.590580938548925 26.632006346430252 27.24579390865661 22.53161880636421 62-63 23.245822882291392 26.7680110866378 27.141008506287434 22.84515752478337 64-65 23.212283274389716 27.109241429952203 26.794595323880237 22.88387997177784 66-67 23.50288293520423 26.69617240065313 26.547574789228694 23.25336987491394 68-69 23.33040589898295 26.699506125385458 26.827536224512027 23.142551751119573 70-71 23.718383792545 26.602961538742232 26.635868999115615 23.042785669597155 72-73 23.608597007373092 26.545072630660105 26.883904396409058 22.962425965557742 74-75 23.279373492878058 27.28667292277321 26.423520424536473 23.010433159812262 76-77 23.56826693453538 26.75303398031413 26.3706045048476 23.30809458030289 78-79 23.51260089384605 26.87011896715841 26.464265620189735 23.153014518805797 80-81 23.76652297563622 26.82368953536951 26.36667376606959 23.04311372292468 82-83 23.678899054162187 26.779135391974425 26.6685303574438 22.87343519641959 84-85 23.45927676266541 26.986531748236402 26.473112153451726 23.081079335646457 86-87 23.458937586972272 27.02956681284053 26.355506821103177 23.155988779084012 88-89 23.33424016420274 26.951323088356293 26.149043182843645 23.565393564597322 90-91 23.510794492139304 26.64571602233261 26.391021481045996 23.452468004482093 92-93 23.70299305637107 26.83219294340468 26.52197769024431 22.942836309979945 94-95 23.447544055898277 26.766824971990268 26.57171885788393 23.21391211422753 96-97 23.088943705860213 26.817507697302396 26.86518873152968 23.228359865307713 98-99 23.102690073792694 27.088393320396868 26.212572314227796 23.596344291582643 100-101 22.989925020424646 26.852759859674823 26.284098765497628 23.8732163544029 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 91.0 1 81.5 2 79.0 3 91.5 4 85.0 5 94.0 6 177.5 7 367.5 8 590.0 9 764.0 10 785.0 11 749.0 12 815.0 13 928.5 14 1082.0 15 1230.0 16 1375.0 17 1575.5 18 1832.0 19 2063.0 20 2321.5 21 2667.0 22 3126.5 23 3789.5 24 4797.0 25 6113.5 26 7848.5 27 10187.0 28 13031.0 29 16539.0 30 20493.5 31 24991.5 32 29816.5 33 33827.0 34 37424.0 35 41217.0 36 46571.0 37 51520.0 38 54429.0 39 57790.0 40 61834.5 41 66140.5 42 69338.0 43 72331.5 44 76045.5 45 80004.0 46 83863.5 47 92514.5 48 107012.5 49 105696.5 50 92044.0 51 86424.0 52 85417.5 53 84454.5 54 83024.0 55 78614.5 56 71643.0 57 64838.5 58 60068.5 59 53804.0 60 44675.5 61 36958.5 62 30087.5 63 23289.0 64 16689.5 65 11835.5 66 8997.0 67 7292.5 68 5784.0 69 4577.0 70 3594.0 71 2518.0 72 1799.0 73 1345.0 74 965.0 75 697.0 76 505.0 77 310.0 78 189.0 79 118.0 80 78.0 81 54.5 82 43.5 83 27.5 84 15.0 85 9.5 86 4.0 87 1.0 88 1.0 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035693939726337616 2 0.004776884126944812 3 0.003759584729539898 4 6.192257201595126E-4 5 9.288385802392687E-4 6 3.096128600797563E-4 7 0.0 8 0.0 9 0.0023442116548895832 10-11 3.538432686625786E-4 12-13 0.0011278754188619692 14-15 0.0024547876763466392 16-17 0.0032509350308374406 18-19 0.0 20-21 2.211520429141116E-5 22-23 0.0014596034832331367 24-25 7.961473544908019E-4 26-27 0.004135543202493887 28-29 0.005152842599898801 30-31 0.0010394146016963245 32-33 4.201888815368121E-4 34-35 0.007253787007582862 36-37 0.010947026124248525 38-39 0.007762436706285318 40-41 0.001349027461776081 42-43 3.7595847295398974E-4 44-45 0.0015701795046901925 46-47 4.423040858282232E-5 48-49 5.971105158681015E-4 50-51 0.001083645010279147 52-53 0.0030518981922147406 54-55 0.0038922759552883646 56-57 0.0015480643003987814 58-59 9.288385802392687E-4 60-61 0.0039586215681625975 62-63 0.006568215674549115 64-65 0.011190293371454049 66-67 0.003737469525248486 68-69 0.004047082385328243 70-71 1.1057602145705581E-4 72-73 0.0013932578703589032 74-75 0.00723167180329145 76-77 0.0034942022780429633 78-79 0.004246119223950943 80-81 0.005948989954389603 82-83 0.006236487610177948 84-85 0.007253787007582862 86-87 0.004865344944110455 88-89 0.011853749500196385 90-91 0.014905647692411124 92-93 0.0017028707304386596 94-95 0.002963437375049096 96-97 0.001371142666067492 98-99 0.004201888815368121 100-101 0.006148026793012303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.29646200574644 #Duplication Level Percentage of deduplicated Percentage of total 1 78.17903507802765 47.9209825330624 2 13.04300289701296 15.989798630351912 3 3.914998368399557 7.199266462234881 4 1.7225716982865653 4.223502026247862 5 0.8969884056807204 2.7491107864201676 6 0.5291518006013546 1.946107994449995 7 0.36164640729810527 1.5517351685124108 8 0.23585996513869004 1.1565905113440316 9 0.1850546697239269 1.0208876878546778 >10 0.8616608419534697 9.350532312117762 >50 0.047525535604264 1.9538975800148652 >100 0.02005529310791957 2.1972828154358908 >500 0.0012965501460872363 0.5820141302655808 >1k 0.0010804584550722534 1.7155332295146752 >5k 0.0 0.0 >10k+ 7.203056367148355E-5 0.4427581321727748 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10028 0.4435425372685423 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4525 0.20014259883727104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4069 0.17997353252350404 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3620 0.1601140790698168 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3528 0.15604488148019716 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2841 0.12565859078379824 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2670 0.1180951909161356 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2462 0.10889526593090856 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2428 0.1073914320390926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.846081716564464E-5 2 0.0 0.0 0.0 0.0 8.846081716564464E-5 3 0.0 0.0 0.0 0.0 8.846081716564464E-5 4 0.0 0.0 0.0 0.0 8.846081716564464E-5 5 0.0 0.0 0.0 0.0 8.846081716564464E-5 6 4.423040858282232E-5 0.0 0.0 0.0 8.846081716564464E-5 7 4.423040858282232E-5 0.0 0.0 0.0 8.846081716564464E-5 8 4.423040858282232E-5 0.0 0.0 0.0 8.846081716564464E-5 9 4.423040858282232E-5 0.0 0.0 0.0 8.846081716564464E-5 10-11 6.634561287423348E-5 0.0 0.0 0.0 1.3269122574846698E-4 12-13 8.846081716564464E-5 0.0 0.0 0.0 1.769216343312893E-4 14-15 8.846081716564464E-5 0.0 0.0 0.0 1.769216343312893E-4 16-17 3.3172806437116746E-4 0.0 0.0 0.0 1.769216343312893E-4 18-19 3.538432686625786E-4 0.0 0.0 0.0 1.769216343312893E-4 20-21 3.538432686625786E-4 0.0 0.0 4.423040858282232E-5 1.769216343312893E-4 22-23 3.538432686625786E-4 0.0 0.0 1.3269122574846698E-4 1.769216343312893E-4 24-25 3.538432686625786E-4 0.0 0.0 2.2115204291411162E-4 1.769216343312893E-4 26-27 3.538432686625786E-4 2.211520429141116E-5 0.0 4.4230408582822324E-4 2.6538245149693396E-4 28-29 3.538432686625786E-4 4.423040858282232E-5 0.0 7.961473544908018E-4 2.6538245149693396E-4 30-31 3.980736772454009E-4 4.423040858282232E-5 0.0 0.0017028707304386596 2.6538245149693396E-4 32-33 3.980736772454009E-4 4.423040858282232E-5 0.0 0.0033615110522944966 2.6538245149693396E-4 34-35 4.4230408582822324E-4 4.423040858282232E-5 0.0 0.004599962492613522 2.6538245149693396E-4 36-37 4.4230408582822324E-4 4.423040858282232E-5 0.0 0.006966289351794516 2.6538245149693396E-4 38-39 4.4230408582822324E-4 4.423040858282232E-5 0.0 0.010195109178340546 2.6538245149693396E-4 40-41 4.4230408582822324E-4 4.423040858282232E-5 0.0 0.01464026524091419 2.6538245149693396E-4 42-43 4.4230408582822324E-4 6.634561287423348E-5 0.0 0.020545024786720968 2.6538245149693396E-4 44-45 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.02664882117115045 2.874976557883451E-4 46-47 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.03750738647823333 3.096128600797563E-4 48-49 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.04975920965567512 3.096128600797563E-4 50-51 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.05721203350188068 3.096128600797563E-4 52-53 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.06681003216435313 3.538432686625786E-4 54-55 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.08474546284468756 3.980736772454009E-4 56-57 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.11468944945525829 4.201888815368121E-4 58-59 4.4230408582822324E-4 8.846081716564464E-5 0.0 0.14591611791473086 5.086496987024567E-4 60-61 4.865344944110456E-4 8.846081716564464E-5 0.0 0.18003987813637828 5.307649029938679E-4 62-63 5.749953115766902E-4 8.846081716564464E-5 0.0 0.21546843541121896 5.307649029938679E-4 64-65 5.749953115766902E-4 8.846081716564464E-5 0.0 0.2545902318027253 5.307649029938679E-4 66-67 6.192257201595126E-4 1.1057602145705581E-4 0.0 0.31235514541189124 5.307649029938679E-4 68-69 6.192257201595126E-4 1.3269122574846698E-4 0.0 0.3878343376584775 5.307649029938679E-4 70-71 7.298017416165683E-4 1.3269122574846698E-4 0.0 0.42861477437183976 5.307649029938679E-4 72-73 7.961473544908018E-4 1.3269122574846698E-4 0.0 0.4823989512085517 6.192257201595125E-4 74-75 7.961473544908018E-4 1.3269122574846698E-4 0.0 0.5428840349455613 7.076865373251571E-4 76-77 7.961473544908018E-4 1.3269122574846698E-4 0.0 0.6090084957768807 7.076865373251571E-4 78-79 7.961473544908018E-4 1.3269122574846698E-4 0.0 0.6857703698723687 7.076865373251571E-4 80-81 7.961473544908018E-4 1.3269122574846698E-4 0.0 0.8002607824890042 7.298017416165683E-4 82-83 7.961473544908018E-4 1.3269122574846698E-4 0.0 0.9281530089062351 7.961473544908018E-4 84-85 7.961473544908018E-4 1.3269122574846698E-4 0.0 1.1134341904596778 9.067233759478576E-4 86-87 7.961473544908018E-4 1.3269122574846698E-4 0.0 1.3751455180442376 9.288385802392688E-4 88-89 7.961473544908018E-4 1.3269122574846698E-4 0.0 1.6650095006917636 9.5095378453068E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 6970 0.0 20.853512 2 GTACATG 7225 0.0 20.45301 1 GAGTACT 6375 0.0 20.377611 12-13 ACATGGG 7175 0.0 20.124405 3 AGAGTAC 10270 0.0 19.21682 10-11 GTACTTT 6770 0.0 19.188454 14-15 AGTACTT 6825 0.0 18.616468 12-13 TATACCG 160 0.0011920846 17.811195 5 GTGTAGC 1410 0.0 17.52342 1 ACCGTAT 225 3.54159E-5 16.888025 8 TACTTTT 7480 0.0 16.605099 14-15 GTAGGAC 615 0.0 16.218681 3 ACTTTTT 8060 0.0 16.205387 16-17 CATGGGG 4760 0.0 16.165138 4 GTATAGG 745 0.0 15.944777 1 CGTATGG 180 0.0026139896 15.838477 1 GGACCGT 330 2.2785207E-7 15.832524 6 GTACCGT 270 9.489868E-6 15.832522 6 GTGTAGG 935 0.0 15.753779 1 GTATAGC 820 0.0 15.645326 1 >>END_MODULE