FastQCFastQC Report
Thu 26 May 2016
SRR936572_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936572_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323658
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT73610.31678500020226724No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53890.23191881077163679No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39470.16986148564031367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37130.15979115687420437No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33880.145804589143497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32090.13810121799335356No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24590.10582452323018275No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23500.1011336435912686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG68850.023.5406741
TACATGG68250.022.771672
ACATGGG69700.021.7557053
GAGTACT52300.020.3590212-13
GTGTAGC13850.019.904291
AGAGTAC85550.018.72655510-11
GTATAGG6100.018.7003561
GTACTTT56400.018.62296714-15
GAGTACA55900.018.535881
AGTACTT56600.018.14044212-13
AGTACAT53400.017.8007582
CATGGGG47800.017.5032524
GTGTATA12700.016.4671121
TATAGGG8550.016.1206172
TACTTTT63100.016.04301514-15
ACTTTTT68950.015.54162916-17
GTATCAA153200.015.4193511
GTATATA13400.015.2521881
TACACTG19450.014.9091745
CGTACAC2901.8527608E-514.75233
Produced by FastQC (version 0.11.5)