##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936572_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2323658 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.258067237089108 31.0 31.0 34.0 28.0 34.0 2 31.336218582941207 31.0 31.0 34.0 28.0 34.0 3 31.303763720822943 31.0 31.0 34.0 28.0 34.0 4 34.95894447461718 37.0 35.0 37.0 32.0 37.0 5 34.98244492089628 37.0 35.0 37.0 32.0 37.0 6 35.01684327039521 37.0 35.0 37.0 32.0 37.0 7 34.88335761975299 37.0 35.0 37.0 32.0 37.0 8 34.864418515977825 37.0 35.0 37.0 32.0 37.0 9 36.35226956806897 39.0 37.0 39.0 32.0 39.0 10-11 36.22166342895555 39.0 35.0 39.0 32.0 39.0 12-13 36.12127214934384 39.0 35.0 39.0 31.0 39.0 14-15 37.11244339743628 39.0 36.0 41.0 31.0 41.0 16-17 37.019562259162065 39.0 36.0 41.0 31.0 41.0 18-19 36.873444370901396 39.0 36.0 41.0 30.0 41.0 20-21 36.82740489349121 39.0 36.0 41.0 30.0 41.0 22-23 36.80068129647306 39.0 36.0 41.0 30.0 41.0 24-25 36.92271861866075 39.0 36.0 41.0 30.0 41.0 26-27 36.8356201730203 39.0 36.0 41.0 30.0 41.0 28-29 36.719852921557305 39.0 36.0 41.0 30.0 41.0 30-31 36.54292262458589 39.0 36.0 41.0 30.0 41.0 32-33 36.40140610193066 39.0 35.0 41.0 30.0 41.0 34-35 36.21849837626708 39.0 35.0 40.0 29.5 41.0 36-37 36.01469837643921 38.5 35.0 40.0 28.5 41.0 38-39 35.82682907725663 38.0 35.0 40.0 28.0 41.0 40-41 35.62196459203549 38.0 35.0 40.0 27.0 41.0 42-43 35.3714492408091 38.0 34.0 40.0 26.5 41.0 44-45 35.262993091065894 38.0 34.0 40.0 26.0 41.0 46-47 35.20143024489835 38.0 34.0 40.0 26.0 41.0 48-49 35.004262890666354 38.0 34.0 40.0 25.0 41.0 50-51 34.043768704344615 37.5 33.0 39.5 23.5 40.5 52-53 34.16933688176143 37.5 33.0 39.5 24.0 40.5 54-55 34.6244619474983 38.0 34.0 40.0 24.5 41.0 56-57 34.459024520820186 38.0 34.0 40.0 24.0 41.0 58-59 34.22503892569388 38.0 33.0 40.0 23.5 41.0 60-61 33.91204686748222 37.0 33.0 40.0 23.0 41.0 62-63 33.56764678795244 37.0 33.0 40.0 22.0 41.0 64-65 33.19931375443374 36.0 32.0 39.0 22.0 41.0 66-67 32.8135366736413 36.0 32.0 39.0 20.5 41.0 68-69 32.35228226356891 35.0 31.0 39.0 19.5 40.0 70-71 31.93922298376095 35.0 31.0 38.0 18.5 40.0 72-73 31.50360380055929 35.0 31.0 37.0 17.5 39.5 74-75 31.08154965145473 35.0 30.5 37.0 14.0 39.0 76-77 30.607984049287804 34.5 30.0 36.0 10.0 39.0 78-79 30.134634700975788 34.0 29.5 36.0 7.5 38.0 80-81 29.676285408610045 34.0 29.0 35.0 7.0 37.0 82-83 29.261583029860674 34.0 29.0 35.0 2.0 37.0 84-85 28.88295071822101 34.0 29.0 35.0 2.0 36.0 86-87 28.5376380689413 34.0 28.5 35.0 2.0 36.0 88-89 28.164644711054727 34.0 27.0 35.0 2.0 35.5 90-91 27.78689635049564 33.5 27.0 35.0 2.0 35.0 92-93 27.336805588429968 33.0 26.0 35.0 2.0 35.0 94-95 26.826469084521044 33.0 25.0 35.0 2.0 35.0 96-97 26.199061135502728 33.0 24.5 35.0 2.0 35.0 98-99 25.427488898968782 32.0 21.0 35.0 2.0 35.0 100-101 22.86915178567586 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43460110231659144 1101 2 0.4100623012912692 1101 3 0.4553940318371623 1101 4 0.5652541035598944 1101 5 0.466327900506748 1101 6 0.47027621086333227 1101 7 0.4373382747677681 1101 8 0.37312877675859824 1101 9 0.5099889833458064 1101 10-11 0.6256511927131427 1101 12-13 0.6310965367144803 1101 14-15 0.7560564991883396 1101 16-17 0.8244195084036292 1101 18-19 1.026044070309645 1101 20-21 0.9117373323682401 1101 22-23 0.7197573730632101 1101 24-25 0.6784089862093552 1101 26-27 0.6345380291217566 1101 28-29 0.5788073047008808 1101 30-31 0.4774266921433892 1101 32-33 0.41086875008152646 1101 34-35 0.5344051665140128 1101 36-37 0.5703531282650403 1101 38-39 0.4975428276352929 1101 40-41 0.3859983413603487 1101 42-43 0.5044723651050944 1101 44-45 0.49413089559723744 1101 46-47 0.5801215637671078 1101 48-49 0.5667972283385581 1101 50-51 0.4540545560890905 1101 52-53 0.3523696277487076 1101 54-55 0.4222863636113843 1101 56-57 0.606788362560799 1101 58-59 0.5643118775563778 1101 60-61 0.3962916210890768 1101 62-63 0.5022956539676287 1101 64-65 0.34618756821974017 1101 66-67 0.40489119936581375 1101 68-69 0.5648429518645344 1101 70-71 0.36276348322419594 1101 72-73 0.5241674411690234 1101 74-75 0.3533013515717336 1101 76-77 0.36310661013186163 1101 78-79 0.37827324809572715 1101 80-81 0.2938883867575228 1101 82-83 0.28807970203322597 1101 84-85 0.4272224959787607 1101 86-87 0.31745838133698 1101 88-89 0.29310637269071904 1101 90-91 0.16364082948294367 1101 92-93 0.1716793947317612 1101 94-95 0.17075141397015514 1101 96-97 0.18467772189952925 1101 98-99 -0.002430733520643713 1101 100-101 0.5693082315992157 1102 1 0.18353691370770164 1102 2 0.2531684478932945 1102 3 0.3557483908907493 1102 4 0.28558684356976727 1102 5 0.2697385398025247 1102 6 0.23940051283258157 1102 7 0.2423787930222474 1102 8 0.22883029433536706 1102 9 0.3191042908522732 1102 10-11 0.3013393122880572 1102 12-13 0.21067553302722786 1102 14-15 0.4365913592533488 1102 16-17 0.15008726367708647 1102 18-19 0.3097122679578206 1102 20-21 0.32276742615793097 1102 22-23 0.042957989081031656 1102 24-25 0.24069302311383467 1102 26-27 0.12331603141409886 1102 28-29 0.08925101316050643 1102 30-31 0.10778462987619974 1102 32-33 0.3140573477628479 1102 34-35 -0.024655119717863272 1102 36-37 -0.0024915947170782715 1102 38-39 -0.01418198552660499 1102 40-41 0.23248002937999246 1102 42-43 0.06494823207595601 1102 44-45 0.15333821447390505 1102 46-47 0.20327134710820616 1102 48-49 0.29753987408844296 1102 50-51 0.16743266210433916 1102 52-53 -0.06968768476239262 1102 54-55 0.13926280587147488 1102 56-57 0.12971285261279064 1102 58-59 0.07385861982846365 1102 60-61 0.09894553874161005 1102 62-63 0.07276895082954837 1102 64-65 0.11935579608828562 1102 66-67 0.0561084003940735 1102 68-69 0.1186348821633203 1102 70-71 -0.19528612006231683 1102 72-73 0.07526906996409011 1102 74-75 -0.2019866246355626 1102 76-77 -0.15253238343624886 1102 78-79 -0.03774169050181797 1102 80-81 0.1901142671851801 1102 82-83 -0.030266503583941784 1102 84-85 0.05638130978988798 1102 86-87 -0.2262460091035372 1102 88-89 -0.11089744003559687 1102 90-91 0.13724366112206354 1102 92-93 0.09857789049236487 1102 94-95 0.22172306763410177 1102 96-97 0.2225699275986308 1102 98-99 0.19720376538802142 1102 100-101 0.5691630544297936 1103 1 0.21336592757166528 1103 2 0.31926325266794464 1103 3 0.32698742590895336 1103 4 0.36792133894837065 1103 5 0.33842793889363065 1103 6 0.3160067415679677 1103 7 0.3121296254140091 1103 8 0.22666525340191157 1103 9 0.2314640990996395 1103 10-11 0.27463041799705934 1103 12-13 0.2278824767783405 1103 14-15 0.6011231685772174 1103 16-17 0.6931355876516747 1103 18-19 0.7604097845512214 1103 20-21 0.6333215665609657 1103 22-23 0.48374632933913375 1103 24-25 0.3332366471312298 1103 26-27 0.428395456523738 1103 28-29 0.4055210159914253 1103 30-31 0.4278046567115794 1103 32-33 0.5824463902649271 1103 34-35 0.4469476804005197 1103 36-37 0.5239433042395589 1103 38-39 0.3808411400655274 1103 40-41 0.4044761441959821 1103 42-43 0.518663969910385 1103 44-45 0.5038828560044948 1103 46-47 0.6347654385210291 1103 48-49 0.8004761815667081 1103 50-51 0.7001508933698162 1103 52-53 0.8144271738612545 1103 54-55 0.7299661496895808 1103 56-57 0.7735891166138131 1103 58-59 0.5893837816873955 1103 60-61 0.6059306280478296 1103 62-63 0.635585275548749 1103 64-65 0.7759491373033498 1103 66-67 0.6261865203046142 1103 68-69 0.6055802724674777 1103 70-71 0.7199691995773172 1103 72-73 0.7686132528511145 1103 74-75 0.6576189746088623 1103 76-77 0.5932779090670941 1103 78-79 0.5776732392301405 1103 80-81 0.6528064303030021 1103 82-83 0.4738405780561017 1103 84-85 0.5922863677419237 1103 86-87 0.4349154830298474 1103 88-89 0.3825428893027514 1103 90-91 0.5979383780381191 1103 92-93 0.5692942026879066 1103 94-95 0.6243195639302463 1103 96-97 0.662780828661969 1103 98-99 0.8930304452380717 1103 100-101 1.1684366543912788 1104 1 0.21642433306203301 1104 2 0.2710055634143096 1104 3 0.30845372816274974 1104 4 0.3847246047383237 1104 5 0.36798955444250936 1104 6 0.37281075378599127 1104 7 0.30749425135188346 1104 8 0.3337662632244047 1104 9 0.4814634724342781 1104 10-11 0.40869976283652676 1104 12-13 0.43679000965807546 1104 14-15 0.4860011206236692 1104 16-17 0.5081857501251079 1104 18-19 0.5037963064553992 1104 20-21 0.5609445022904467 1104 22-23 0.46769715086957575 1104 24-25 0.5770838377068443 1104 26-27 0.2235450944643489 1104 28-29 0.12684167564444948 1104 30-31 0.18381806790738864 1104 32-33 0.17898502502011837 1104 34-35 0.18798114349535666 1104 36-37 0.19633755306693246 1104 38-39 0.1776072961252524 1104 40-41 0.3547956460499364 1104 42-43 0.3734596171206235 1104 44-45 0.38431106619529487 1104 46-47 0.49165707248272383 1104 48-49 0.5025562649438342 1104 50-51 0.3358324393003542 1104 52-53 0.3340089346126689 1104 54-55 0.4417316944024279 1104 56-57 0.6437350015251937 1104 58-59 0.4862659961723921 1104 60-61 0.44712540873305073 1104 62-63 0.36245248070199665 1104 64-65 0.3506689552194615 1104 66-67 0.42874515320488626 1104 68-69 0.3357763240300571 1104 70-71 0.2905028928672344 1104 72-73 0.41246651511780286 1104 74-75 0.29189679012582204 1104 76-77 0.33085009047250935 1104 78-79 0.24836045160814635 1104 80-81 0.5972053370643877 1104 82-83 0.4090046655073927 1104 84-85 0.25755097510264946 1104 86-87 0.17832094415389577 1104 88-89 0.16471275572276411 1104 90-91 0.32135418888878675 1104 92-93 0.27942860708982664 1104 94-95 0.45322460128925357 1104 96-97 0.31537384460182594 1104 98-99 0.04482996356914626 1104 100-101 0.7283589534565991 1105 1 0.23807072118569295 1105 2 0.16941197602526614 1105 3 0.2955596357232011 1105 4 0.3838158964432523 1105 5 0.3434735611765163 1105 6 0.33629981102505013 1105 7 0.3124395375471707 1105 8 0.173403162520394 1105 9 0.21246668639204103 1105 10-11 0.4185759872013861 1105 12-13 0.27264381284143013 1105 14-15 0.5011717664761335 1105 16-17 0.5469254195466036 1105 18-19 0.38178547847866184 1105 20-21 0.29769649188334313 1105 22-23 0.2173288848763022 1105 24-25 0.13853643182989828 1105 26-27 0.2073700868120696 1105 28-29 0.3260496646251667 1105 30-31 0.2061196825385494 1105 32-33 0.31108729774739174 1105 34-35 0.19455253927130656 1105 36-37 0.22069571805009502 1105 38-39 0.10233755324186689 1105 40-41 0.2026874428053702 1105 42-43 -0.03461736482000077 1105 44-45 0.164044958145098 1105 46-47 0.37696852029417016 1105 48-49 0.4917139401810573 1105 50-51 0.46300377385804836 1105 52-53 0.5491767108599959 1105 54-55 0.4778839365205769 1105 56-57 0.41170351239142633 1105 58-59 0.4006656747766115 1105 60-61 0.2725147184973622 1105 62-63 0.40168476567291833 1105 64-65 0.47343066320843974 1105 66-67 0.4419453368596109 1105 68-69 0.43634641891833326 1105 70-71 0.4724358588911066 1105 72-73 0.3513200122099356 1105 74-75 0.3863115819837972 1105 76-77 0.41762694997663985 1105 78-79 0.3163509627804757 1105 80-81 0.3834914940891814 1105 82-83 0.36603711117620463 1105 84-85 0.4805766867647243 1105 86-87 0.4529660696512927 1105 88-89 0.46573433515343154 1105 90-91 0.45618258650127785 1105 92-93 0.5595211996824183 1105 94-95 0.5646418036155474 1105 96-97 0.8418029079627054 1105 98-99 0.9770881515091538 1105 100-101 1.3061874275918868 1106 1 0.23473412526304926 1106 2 0.1599595997517227 1106 3 0.08334058791220045 1106 4 0.3502948907427452 1106 5 0.39560077360677326 1106 6 0.20213716275988958 1106 7 0.4610965642491749 1106 8 0.4342997699313855 1106 9 0.4071309462167605 1106 10-11 0.5146406095640188 1106 12-13 0.5431497850174551 1106 14-15 0.32149694207650015 1106 16-17 0.15453649034772354 1106 18-19 0.4020979837273515 1106 20-21 0.11988400972891355 1106 22-23 0.03839134477626516 1106 24-25 -0.07621747361967124 1106 26-27 -0.1768188049321182 1106 28-29 0.04786479800082333 1106 30-31 0.0979053791286546 1106 32-33 0.22703788484550813 1106 34-35 0.17405480754768377 1106 36-37 -0.05629743219193273 1106 38-39 0.03088170359139042 1106 40-41 -0.05144663995774579 1106 42-43 0.026523852810250048 1106 44-45 -0.04700840681478269 1106 46-47 0.07224288851915617 1106 48-49 -0.05162742785581287 1106 50-51 -0.08403304343358542 1106 52-53 0.06503517284936322 1106 54-55 0.20738583738825156 1106 56-57 0.3043763435463376 1106 58-59 0.27437565032188616 1106 60-61 0.24076236582195065 1106 62-63 0.1842583292938329 1106 64-65 0.011253395016048273 1106 66-67 -0.034348613619570756 1106 68-69 0.060681094238205446 1106 70-71 -0.16905932238625 1106 72-73 0.019814852112119752 1106 74-75 0.17639550443074725 1106 76-77 0.16109757239638256 1106 78-79 0.15553806157016936 1106 80-81 0.32902698493041527 1106 82-83 0.1735136893960494 1106 84-85 0.3095754371552317 1106 86-87 0.4074200394288816 1106 88-89 0.4392017579682097 1106 90-91 0.7290861629206091 1106 92-93 0.8066585609908827 1106 94-95 0.8533351890831256 1106 96-97 0.855455710514331 1106 98-99 0.9098181367624179 1106 100-101 1.5132674248541704 1107 1 0.20929594352324088 1107 2 0.18318914496109784 1107 3 0.3180181021877111 1107 4 0.3365905791331443 1107 5 0.4523670206198389 1107 6 0.29953716271521813 1107 7 0.5282941464298148 1107 8 0.31507784249679105 1107 9 0.4687217318092749 1107 10-11 0.5763032556888064 1107 12-13 0.3725465719638734 1107 14-15 0.27473980074766047 1107 16-17 0.2627043471707253 1107 18-19 0.5793359284111617 1107 20-21 0.24559577517970155 1107 22-23 0.41969304443698974 1107 24-25 0.2994215379278202 1107 26-27 0.26729162421428754 1107 28-29 0.41024919858124775 1107 30-31 0.23204984124447492 1107 32-33 0.43530578805680165 1107 34-35 0.2432211003013478 1107 36-37 0.32968128815680586 1107 38-39 0.32042783313957557 1107 40-41 0.29460180129964897 1107 42-43 0.06935172500369191 1107 44-45 0.13238083114643473 1107 46-47 0.22265803803708195 1107 48-49 0.1741319542709121 1107 50-51 0.1901122070821657 1107 52-53 0.21643727401335155 1107 54-55 0.3296005019397157 1107 56-57 0.529712323640247 1107 58-59 0.53908076082854 1107 60-61 0.4300518298730367 1107 62-63 0.4780788483736629 1107 64-65 0.4431136414122392 1107 66-67 0.40771705674482916 1107 68-69 0.42532069831403874 1107 70-71 0.3961143835602279 1107 72-73 0.4286701802774502 1107 74-75 0.4640350685282222 1107 76-77 0.48866981651984176 1107 78-79 0.5990478103698678 1107 80-81 0.45570593619322963 1107 82-83 0.717278707800606 1107 84-85 0.5632155332551498 1107 86-87 0.7274451409214144 1107 88-89 0.6744369591095811 1107 90-91 0.6583640086814384 1107 92-93 0.7301169320226109 1107 94-95 0.798703372969257 1107 96-97 1.0684593735588983 1107 98-99 1.0584698835372954 1107 100-101 1.3735228664329533 1108 1 0.09825805558775969 1108 2 0.019566023851062653 1108 3 0.19037201372808354 1108 4 0.22354655163587722 1108 5 0.13596937716149426 1108 6 0.1477818707850247 1108 7 0.31564828742656204 1108 8 0.20127858277734134 1108 9 -0.26510615814443383 1108 10-11 -0.03662826416607601 1108 12-13 -0.01770259298762511 1108 14-15 0.3302482588057458 1108 16-17 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-5.699940515322332 2316 92-93 -4.950919617889269 2316 94-95 -4.3585396756062025 2316 96-97 -5.856164602714536 2316 98-99 -7.2627693157812026 2316 100-101 -7.901732586305474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6518.0 3 2633.0 4 2612.0 5 2854.0 6 3096.0 7 3448.0 8 4552.0 9 5596.0 10 6942.0 11 8251.0 12 9778.0 13 11201.0 14 12268.0 15 13943.0 16 16288.0 17 18452.0 18 16501.0 19 15030.0 20 15590.0 21 17508.0 22 19733.0 23 22014.0 24 25477.0 25 29457.0 26 34457.0 27 40174.0 28 48020.0 29 57478.0 30 69619.0 31 83873.0 32 103622.0 33 131222.0 34 170783.0 35 230369.0 36 318308.0 37 406829.0 38 300071.0 39 39079.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18985953781075 17.875228368418014 14.199368966702355 23.73554312706888 2 15.63722179245279 19.141729636139548 40.14027846476314 25.080770106644522 3 20.2749868614232 22.50809297592961 29.03545844701807 28.18146171562912 4 13.088834183966656 15.572594527049452 36.76027292007347 34.578298368910424 5 14.42815559808563 36.505870269497755 34.12694108034999 14.939033052066625 6 33.345688642758496 34.64824821515286 17.17138320917754 14.834679932911099 7 29.61248116200952 31.416750403032857 20.14053065526273 18.830237779694897 8 26.393659055834206 34.24448306509398 19.422521771482117 19.939336107589703 9 25.713070014582247 17.084869005280787 20.256700898621105 36.945360081515865 10-11 25.582695236358383 25.59681974606457 27.017560253629686 21.802924763947363 12-13 26.398710123229296 22.831744487294433 27.34190022183211 23.427645167644158 14-15 23.025850005090913 24.607197756295243 25.67002566552672 26.696926573087126 16-17 22.767596106565673 27.621665517030547 26.34961683101727 23.261121545386516 18-19 22.611867345135277 26.91504955580714 27.922010123640334 22.551072975417245 20-21 23.468645471093797 25.795716378252436 27.81091424362986 22.92472390702391 22-23 23.016413933001818 25.679538031425587 27.93968997076004 23.364358064812556 24-25 23.20364674915471 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27.27919243979599 23.254108530534623 50-51 22.852318528756292 26.412703003198217 27.553155297971138 23.18182317007436 52-53 23.243843532642984 26.623972415858816 26.831275224280375 23.30090882721782 54-55 22.925482252202293 26.190145738103492 27.13871619038028 23.745655819313928 56-57 23.27019570055272 26.26309336241463 26.951937961810142 23.514772975222513 58-59 23.59814054455112 26.183177837164017 26.91941133167799 23.29927028660687 60-61 23.204001284239574 26.248862733553857 27.539751604653905 23.00738437755266 62-63 22.943421967158788 26.346582142834084 27.4319215185025 23.278074371504623 64-65 22.83137427382535 26.65643563205975 27.088493141606158 23.42369695250875 66-67 22.991243955635355 26.39188226119918 26.786455114621294 23.83041866854417 68-69 22.884049235666225 26.306987189267154 27.17322280750719 23.635740767559426 70-71 23.29900789402617 26.131647438180316 26.95735160102298 23.611993066770527 72-73 23.06843183965975 26.097409639552527 27.324440606841527 23.5097179139462 74-75 22.816917291695855 26.829672471147077 26.875168268811727 23.47824196834534 76-77 23.05816821560938 26.341467614255155 26.819387109410307 23.780977060725156 78-79 23.000992584455766 26.388025626412905 26.975010631758916 23.635971157372417 80-81 23.279854865679734 26.326535004185743 26.793573114857118 23.60003701527741 82-83 23.248013179764516 26.275326416940885 27.099405998204478 23.377254405090117 84-85 23.08845532045195 26.50298428872103 26.860642743725162 23.54791764710186 86-87 23.03039757964127 26.551150197590186 26.914935623148768 23.503516599619775 88-89 22.980047176165236 26.40787199000185 26.607408835568126 24.004671998264783 90-91 22.972609186564693 26.211360321957777 26.868398123634492 23.947632367843042 92-93 23.191132269434636 26.31766623175035 27.061952010288216 23.429249488526796 94-95 22.963103244202937 26.20648840587507 27.09379673965675 23.73661161026524 96-97 22.51901571291304 26.2554459344734 27.419167752535877 23.806370600077685 98-99 22.666899971288895 26.460123203780395 26.795401746685414 24.077575078245292 100-101 22.67550221893173 26.144611504009575 26.927328371764688 24.252557905294015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 85.0 1 63.0 2 66.5 3 100.5 4 97.0 5 96.5 6 159.5 7 316.5 8 548.5 9 718.5 10 719.0 11 712.0 12 772.5 13 823.5 14 915.0 15 1051.5 16 1205.5 17 1354.5 18 1530.0 19 1754.5 20 2053.5 21 2429.5 22 2844.0 23 3516.0 24 4471.0 25 5847.0 26 7751.5 27 10166.5 28 12952.0 29 16425.5 30 20602.5 31 25211.5 32 30369.0 33 34576.0 34 38321.5 35 42338.0 36 47461.5 37 52436.5 38 55763.5 39 59521.5 40 63603.5 41 67837.0 42 71343.0 43 75126.5 44 78828.5 45 82682.0 46 86734.0 47 94654.0 48 108405.0 49 108392.0 50 96107.5 51 89946.5 52 88666.0 53 87818.0 54 85822.5 55 80576.0 56 73649.0 57 67275.5 58 61923.5 59 54964.5 60 46144.0 61 38623.0 62 31221.0 63 24167.5 64 17576.5 65 12641.0 66 9654.0 67 7566.5 68 6033.0 69 4739.5 70 3604.5 71 2589.5 72 1888.0 73 1422.0 74 1058.0 75 752.0 76 534.5 77 336.0 78 200.0 79 127.5 80 87.5 81 59.5 82 39.5 83 26.5 84 25.0 85 20.0 86 12.5 87 11.0 88 9.0 89 6.5 90 5.5 91 2.5 92 3.0 93 4.5 94 3.5 95 1.5 96 1.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07539835896676705 2 0.0304261642634157 3 0.014933350777093703 4 0.020958333799552257 5 0.02564921343846642 6 0.03313740662352205 7 0.0812081640241378 8 0.05827019294577774 9 0.0712669420370812 10-11 0.06255223445102506 12-13 0.041120509128279636 14-15 0.038409266768173286 16-17 0.05095414213279235 18-19 0.04624174469736941 20-21 0.018806554148674205 22-23 0.012760053329706868 24-25 0.021216547357657625 26-27 0.023540469380605923 28-29 0.005723733871335627 30-31 9.683008428951247E-4 32-33 0.008305869452389293 34-35 0.01011336435912686 36-37 0.003313740662352205 38-39 0.00703631945837124 40-41 0.0035504364239487916 42-43 0.0014201745695795165 44-45 2.5821355810536664E-4 46-47 3.8732033715805E-4 48-49 7.316050812985387E-4 50-51 7.961584708248804E-4 52-53 1.7214237207024443E-4 54-55 1.5062457556146386E-4 56-57 0.002453028802000983 58-59 0.0032061516798083025 60-61 0.004475701673826355 62-63 0.0022808864299307383 64-65 3.8732033715805E-4 66-67 2.5821355810536664E-4 68-69 0.004239005912229769 70-71 0.0028188313426502523 72-73 0.019301463468376155 74-75 0.015772544840936147 76-77 0.024444216833974707 78-79 0.018505304997551274 80-81 0.012609428754145405 82-83 0.004131416929685866 84-85 0.00312008049377318 86-87 0.0015277635521234192 88-89 0.0011619610114741497 90-91 0.005314895737668797 92-93 0.009575419446407345 94-95 0.012179072823969793 96-97 0.010780416050899057 98-99 0.022163330404043967 100-101 0.0201406575322186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2323658.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.47898930375754 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68029835944722 50.5802480727956 2 12.189570619042453 15.47563245887186 3 3.7187404719849897 7.081856599337562 4 1.5773286582210346 4.005089160948931 5 0.8082313767415519 2.56528554595691 6 0.47888055014580194 1.823931199228973 7 0.31947972167697136 1.4196174884569928 8 0.21758441513147486 1.1049631008636187 9 0.1513092070080775 0.864445997990321 >10 0.7975715428081004 8.954793097567622 >50 0.04183697519023128 1.8127971499507856 >100 0.01706446344722654 1.9212021489704898 >500 9.500305860975577E-4 0.43003185389073023 >1k 0.0010178899136743906 1.4108818301579935 >5k 1.357186551565854E-4 0.5492242950115842 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7361 0.31678500020226724 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5389 0.23191881077163679 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3947 0.16986148564031367 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3713 0.15979115687420437 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3388 0.145804589143497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3209 0.13810121799335356 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2459 0.10582452323018275 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2350 0.1011336435912686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3035593017561106E-5 2 0.0 0.0 0.0 0.0 8.607118603512221E-5 3 0.0 0.0 0.0 0.0 8.607118603512221E-5 4 0.0 0.0 0.0 0.0 8.607118603512221E-5 5 0.0 0.0 0.0 0.0 8.607118603512221E-5 6 2.1517796508780553E-4 0.0 0.0 0.0 8.607118603512221E-5 7 2.1517796508780553E-4 0.0 0.0 0.0 8.607118603512221E-5 8 2.1517796508780553E-4 0.0 0.0 0.0 8.607118603512221E-5 9 2.1517796508780553E-4 0.0 0.0 0.0 8.607118603512221E-5 10-11 2.1517796508780553E-4 0.0 0.0 0.0 8.607118603512221E-5 12-13 2.1517796508780553E-4 0.0 0.0 0.0 1.7214237207024443E-4 14-15 2.1517796508780553E-4 0.0 0.0 0.0 1.93660168579025E-4 16-17 2.5821355810536664E-4 0.0 0.0 0.0 3.012491511229277E-4 18-19 2.5821355810536664E-4 0.0 0.0 0.0 3.012491511229277E-4 20-21 2.5821355810536664E-4 2.1517796508780553E-5 0.0 4.3035593017561106E-5 3.012491511229277E-4 22-23 2.5821355810536664E-4 4.3035593017561106E-5 0.0 8.607118603512221E-5 3.227669476317083E-4 24-25 2.5821355810536664E-4 4.3035593017561106E-5 0.0 1.5062457556146386E-4 3.4428474414048885E-4 26-27 2.5821355810536664E-4 4.3035593017561106E-5 0.0 3.012491511229277E-4 3.4428474414048885E-4 28-29 2.5821355810536664E-4 4.3035593017561106E-5 0.0 8.391940638424416E-4 3.4428474414048885E-4 30-31 2.5821355810536664E-4 4.3035593017561106E-5 0.0 0.0018074949067375665 3.4428474414048885E-4 32-33 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.003442847441404888 4.3035593017561106E-4 34-35 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.005207306755124893 4.3035593017561106E-4 36-37 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.007100872847897581 4.3035593017561106E-4 38-39 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.009532383853389784 4.7339152319317214E-4 40-41 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.013211927056391258 4.7339152319317214E-4 42-43 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.018139502456902004 5.164271162107333E-4 44-45 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.02306707785741275 5.594627092282943E-4 46-47 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.03302981764097815 6.024983022458554E-4 48-49 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.0442621074185616 7.100872847897583E-4 50-51 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.050351643830546494 7.316050812985387E-4 52-53 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.059991616666480174 7.746406743160999E-4 54-55 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.07778683437924169 7.746406743160999E-4 56-57 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.10416765289900665 7.746406743160999E-4 58-59 2.5821355810536664E-4 1.2910677905268332E-4 0.0 0.13472292394147506 7.746406743160999E-4 60-61 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.17089433987273514 7.746406743160999E-4 62-63 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.20525826089725768 8.17676267333661E-4 64-65 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.24386118783401 9.467830463863443E-4 66-67 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.3003884392625765 9.467830463863443E-4 68-69 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.37247305756699134 9.467830463863443E-4 70-71 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.41221642771870903 9.898186394039054E-4 72-73 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.46910948168792477 0.001011336435912686 74-75 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.5296390432671245 0.0011619610114741497 76-77 3.4428474414048885E-4 1.2910677905268332E-4 0.0 0.592643151444834 0.0012049966044917109 78-79 3.4428474414048885E-4 1.7214237207024443E-4 0.0 0.6701932900624791 0.0012049966044917109 80-81 3.4428474414048885E-4 1.7214237207024443E-4 0.0 0.7838072556288405 0.0012049966044917109 82-83 3.4428474414048885E-4 1.7214237207024443E-4 0.0 0.9126773389199272 0.0012695499940180526 84-85 3.4428474414048885E-4 1.7214237207024443E-4 0.0 1.1016035922670204 0.0012910677905268331 86-87 3.4428474414048885E-4 1.7214237207024443E-4 0.0 1.3571489436052981 0.0013341033835443943 88-89 3.4428474414048885E-4 1.7214237207024443E-4 0.0 1.6440026888638517 0.0013771389765619554 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6885 0.0 23.540674 1 TACATGG 6825 0.0 22.77167 2 ACATGGG 6970 0.0 21.755705 3 GAGTACT 5230 0.0 20.35902 12-13 GTGTAGC 1385 0.0 19.90429 1 AGAGTAC 8555 0.0 18.726555 10-11 GTATAGG 610 0.0 18.700356 1 GTACTTT 5640 0.0 18.622967 14-15 GAGTACA 5590 0.0 18.53588 1 AGTACTT 5660 0.0 18.140442 12-13 AGTACAT 5340 0.0 17.800758 2 CATGGGG 4780 0.0 17.503252 4 GTGTATA 1270 0.0 16.467112 1 TATAGGG 855 0.0 16.120617 2 TACTTTT 6310 0.0 16.043015 14-15 ACTTTTT 6895 0.0 15.541629 16-17 GTATCAA 15320 0.0 15.419351 1 GTATATA 1340 0.0 15.252188 1 TACACTG 1945 0.0 14.909174 5 CGTACAC 290 1.8527608E-5 14.7523 3 >>END_MODULE