FastQCFastQC Report
Thu 26 May 2016
SRR936572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936572_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323658
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101200.4355202013377184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44980.19357409739298986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42180.18152413134807274No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38280.16474025007122392No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36330.15634830943279948No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28550.12286661806513695No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27250.11727199097285401No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25660.11042933168306178No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25650.11038629609004423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23470.10100453681221591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74000.023.3038221
TACATGG74450.022.0753482
GAGTACT67750.020.36377112-13
ACATGGG76800.020.2835243
GTACTTT70800.019.4525614-15
GTATAGC8100.018.7679671
CATGGGG51000.018.3445954
AGAGTAC105850.018.0134710-11
ACGGTCC3251.006083E-817.534798
AGTACTT73100.017.41160412-13
TTACGCG1002.7597576E-416.62724580-81
TACGCCC2002.765815E-416.6218784
GAGTACA61550.016.5944421
ACTTTTT83350.016.52376716-17
GTACCTG18250.016.1391661
TACTTTT80600.015.87946214-15
CGGTCCG1800.002623349415.830029
GTGTAGC12700.015.7107831
AGTACAT58300.015.6433162
TACACTG18900.015.0765335
Produced by FastQC (version 0.11.5)