##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936572_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2323658 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912498310852975 31.0 31.0 34.0 30.0 34.0 2 32.08350626469127 33.0 31.0 34.0 30.0 34.0 3 32.17389349035013 33.0 31.0 34.0 30.0 34.0 4 35.86034691852243 37.0 35.0 37.0 35.0 37.0 5 35.68985926500371 37.0 35.0 37.0 33.0 37.0 6 35.76709094023303 37.0 35.0 37.0 33.0 37.0 7 35.710126447179405 37.0 35.0 37.0 33.0 37.0 8 35.673827645892814 37.0 35.0 37.0 33.0 37.0 9 37.31749336606334 39.0 37.0 39.0 34.0 39.0 10-11 37.20678645480531 39.0 37.0 39.0 33.0 39.0 12-13 37.1071439945121 39.0 37.0 39.0 33.0 39.0 14-15 38.25640305070712 40.0 38.0 41.0 33.0 41.0 16-17 38.149649389023686 40.0 37.0 41.0 33.0 41.0 18-19 38.26413374945883 40.0 38.0 41.0 33.5 41.0 20-21 38.267522587231 40.0 38.0 41.0 33.5 41.0 22-23 38.18298755668863 40.0 37.5 41.0 33.0 41.0 24-25 38.05275582723448 40.0 37.0 41.0 33.0 41.0 26-27 37.85587896325535 40.0 37.0 41.0 33.0 41.0 28-29 37.79680249847439 40.0 37.0 41.0 32.5 41.0 30-31 37.82734894722029 40.0 37.0 41.0 33.0 41.0 32-33 37.70466436971361 40.0 37.0 41.0 33.0 41.0 34-35 37.55659158964013 40.0 37.0 41.0 32.0 41.0 36-37 37.37952680644054 39.5 36.0 41.0 32.0 41.0 38-39 37.227760066240386 39.0 36.0 41.0 31.0 41.0 40-41 37.07563290294871 39.0 36.0 41.0 31.0 41.0 42-43 36.837897186246856 39.0 36.0 41.0 31.0 41.0 44-45 36.61019887608245 39.0 35.0 40.0 30.0 41.0 46-47 36.31554837243691 39.0 35.0 40.0 30.0 41.0 48-49 35.99709143944591 39.0 35.0 40.0 29.0 41.0 50-51 35.72731916658992 38.0 35.0 40.0 28.5 41.0 52-53 35.38117872767852 38.0 34.0 40.0 27.5 41.0 54-55 35.20472870792518 38.0 34.0 40.0 27.0 41.0 56-57 34.87634776718433 38.0 34.0 40.0 26.5 41.0 58-59 34.50888964727167 37.5 33.5 40.0 26.0 41.0 60-61 34.067267429199994 37.0 33.0 39.5 26.0 41.0 62-63 33.733530708908106 36.0 32.5 39.0 25.0 40.5 64-65 33.300367136644034 36.0 32.0 39.0 23.5 40.0 66-67 32.8623680851485 35.5 31.5 38.5 23.0 40.0 68-69 32.48441552070055 35.0 31.0 38.0 22.0 40.0 70-71 32.089965692025245 35.0 31.0 37.5 22.0 39.5 72-73 31.620639956482407 34.5 31.0 37.0 20.5 39.0 74-75 31.10958217603451 34.0 30.0 36.0 19.5 39.0 76-77 29.61507803644082 32.5 28.5 35.0 18.0 37.0 78-79 30.402571720967543 34.0 30.0 35.0 18.0 37.0 80-81 30.174877068828543 34.0 30.0 35.0 17.0 37.0 82-83 29.877086042782544 34.0 29.0 35.0 15.0 36.5 84-85 29.50910955915199 34.0 29.0 35.0 9.5 36.0 86-87 29.1399388378152 34.0 29.0 35.0 7.0 36.0 88-89 28.67535605497883 33.0 29.0 35.0 2.0 35.0 90-91 28.186071487284273 33.0 27.5 35.0 2.0 35.0 92-93 27.528910020321405 33.0 26.5 34.5 2.0 35.0 94-95 26.97557579471678 32.0 25.5 34.0 2.0 35.0 96-97 26.247850372128774 32.0 25.0 34.0 2.0 35.0 98-99 25.363919087920856 32.0 22.0 34.0 2.0 35.0 100-101 22.13509518182108 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2648127940891385 1101 2 0.32001804613441465 1101 3 0.22323210260118032 1101 4 0.17280146684396414 1101 5 0.14300766535443898 1101 6 0.1955280251411935 1101 7 0.22138828272167643 1101 8 0.21730890803451786 1101 9 0.15730481419708298 1101 10-11 0.2613805722030875 1101 12-13 0.26656163622891427 1101 14-15 0.3196702568883225 1101 16-17 0.36642202874177343 1101 18-19 0.34917027850626425 1101 20-21 0.19889312624774647 1101 22-23 0.22998085893669895 1101 24-25 0.16354717783259076 1101 26-27 0.18424064997643086 1101 28-29 0.11564319571724724 1101 30-31 0.26515846112430097 1101 32-33 0.22196085923705766 1101 34-35 0.221837719269935 1101 36-37 0.25007224589386823 1101 38-39 0.27560357624573584 1101 40-41 0.35337951602032547 1101 42-43 0.27564452098359027 1101 44-45 0.3983702742637547 1101 46-47 0.20999047137018323 1101 48-49 0.3116355809063762 1101 50-51 0.29392048062695864 1101 52-53 0.38552084873011694 1101 54-55 0.16796693989117983 1101 56-57 0.23214108936056022 1101 58-59 0.15162923898253666 1101 60-61 0.2914216349644647 1101 62-63 0.2821106278629202 1101 64-65 0.1861174570028581 1101 66-67 -0.037955479129152536 1101 68-69 0.06494737303835763 1101 70-71 -0.020436456778771372 1101 72-73 0.26045277819719104 1101 74-75 0.028651823735039272 1101 76-77 -0.0843254740475281 1101 78-79 0.15883493776363267 1101 80-81 0.14081909582931829 1101 82-83 0.2781155507062856 1101 84-85 0.18097363617323126 1101 86-87 0.1753698689161638 1101 88-89 0.16919912278714122 1101 90-91 0.22500579153633637 1101 92-93 0.29679981909611897 1101 94-95 0.18900846286032547 1101 96-97 0.24045238017326298 1101 98-99 0.13872355012951587 1101 100-101 0.8092514017127144 1102 1 0.12109649002706391 1102 2 0.14247688535269987 1102 3 0.12173539533512212 1102 4 0.1255535317951555 1102 5 0.08382985037325597 1102 6 0.03109910463656007 1102 7 0.04426350163562631 1102 8 0.04991150250346976 1102 9 0.17781599215021515 1102 10-11 0.13603209647610726 1102 12-13 0.1544831621130669 1102 14-15 0.13970384282961845 1102 16-17 0.21710200535175517 1102 18-19 0.028905309275010893 1102 20-21 0.11247459052644615 1102 22-23 0.1083223450328461 1102 24-25 0.14461177611574527 1102 26-27 0.09928692939008954 1102 28-29 0.010798044322392286 1102 30-31 0.03003533196245911 1102 32-33 0.10060390600844471 1102 34-35 0.07319999642688657 1102 36-37 0.04644686747334248 1102 38-39 0.08859912365598177 1102 40-41 0.06797791858462432 1102 42-43 0.164138350793138 1102 44-45 0.11230217537830356 1102 46-47 0.1541352432743892 1102 48-49 0.37958717651415697 1102 50-51 0.3441030979825612 1102 52-53 0.3804931993914451 1102 54-55 0.2793299996545713 1102 56-57 0.10683633671655457 1102 58-59 0.24168221004870105 1102 60-61 0.34085085583566155 1102 62-63 0.2865438820966588 1102 64-65 0.08469698956636051 1102 66-67 0.08677614289251068 1102 68-69 0.14237227267275188 1102 70-71 0.2603890515617735 1102 72-73 0.25365328766296 1102 74-75 0.31802937420994226 1102 76-77 0.0206582868687768 1102 78-79 0.29741648930478704 1102 80-81 0.2906776663527175 1102 82-83 0.3252732380050425 1102 84-85 0.13115793447929036 1102 86-87 0.2864576845237998 1102 88-89 0.19512856438642245 1102 90-91 0.17347354548707372 1102 92-93 0.21167391590674 1102 94-95 0.040269974844949985 1102 96-97 0.2783624057843319 1102 98-99 0.26252394396794543 1102 100-101 0.9979591715066398 1103 1 0.11593073157866485 1103 2 0.17029237347648518 1103 3 0.10899093696426831 1103 4 0.10698056234453901 1103 5 0.21057878730903212 1103 6 0.08783212650463668 1103 7 0.12065922624256586 1103 8 0.010714917099114984 1103 9 0.1659280678192374 1103 10-11 0.17173092286275704 1103 12-13 0.19204652583121629 1103 14-15 0.18834573285715095 1103 16-17 0.1342316938120831 1103 18-19 0.13808895846101166 1103 20-21 0.11151749045925641 1103 22-23 0.12226624508537043 1103 24-25 0.11112636710236501 1103 26-27 0.06002997680441524 1103 28-29 0.006715265756106703 1103 30-31 0.08622220082335019 1103 32-33 0.057225170490482924 1103 34-35 0.06615610195166255 1103 36-37 -0.024706100667025055 1103 38-39 0.03157382172130241 1103 40-41 0.059436605610379445 1103 42-43 0.12809441157085644 1103 44-45 0.10566082066157634 1103 46-47 0.16387563039800312 1103 48-49 0.13700124493414734 1103 50-51 0.1874446116662014 1103 52-53 0.29898725965590245 1103 54-55 0.22629173412037318 1103 56-57 0.12175093553161531 1103 58-59 0.24309978215416805 1103 60-61 0.21355631719115564 1103 62-63 0.28315863390623264 1103 64-65 0.27678462543961047 1103 66-67 0.04098102028510908 1103 68-69 0.055385185828455974 1103 70-71 0.020812658272070905 1103 72-73 0.03367541678312591 1103 74-75 -0.16649884799862136 1103 76-77 -0.09782778875632303 1103 78-79 -0.19618050067912662 1103 80-81 0.01139000460519668 1103 82-83 0.028679950082160843 1103 84-85 0.3978821015752807 1103 86-87 0.19061596341411402 1103 88-89 0.07253618812742957 1103 90-91 0.21116572927858712 1103 92-93 0.20666743677159616 1103 94-95 0.0348264538560521 1103 96-97 0.32688034554156076 1103 98-99 0.35223430737745787 1103 100-101 0.6208554510414217 1104 1 0.09132367165024036 1104 2 0.14737898113634174 1104 3 0.1279621863929563 1104 4 0.1739165001548244 1104 5 0.1149212440545 1104 6 0.0626003508385864 1104 7 0.029709471714348012 1104 8 0.07395046556917606 1104 9 0.1430277658222323 1104 10-11 0.1558336759290242 1104 12-13 0.12485184485541367 1104 14-15 0.2533140806859464 1104 16-17 0.283937314276244 1104 18-19 0.1294003128296879 1104 20-21 0.23534809807677703 1104 22-23 0.08284662633744233 1104 24-25 0.15856590990959774 1104 26-27 0.24095926861963335 1104 28-29 0.24368208842066963 1104 30-31 0.15878002323641027 1104 32-33 0.19896100012439888 1104 34-35 0.08325459675716473 1104 36-37 0.08623065821414144 1104 38-39 0.17947589927016594 1104 40-41 0.1325276506180444 1104 42-43 0.028275671045690842 1104 44-45 0.17738615295436944 1104 46-47 0.2962287476961123 1104 48-49 0.3632573817287934 1104 50-51 0.3566449560257823 1104 52-53 0.29955863920817904 1104 54-55 0.22771663189733005 1104 56-57 0.14348285477366574 1104 58-59 0.24511861943647517 1104 60-61 0.3255873730554484 1104 62-63 0.28138015760546153 1104 64-65 0.21452862456171573 1104 66-67 0.15361562452365263 1104 68-69 0.14336106506085144 1104 70-71 0.18172096654574688 1104 72-73 0.3748593352687237 1104 74-75 0.08183067103153618 1104 76-77 0.09273878079113729 1104 78-79 0.1898839377883199 1104 80-81 0.4312815608603877 1104 82-83 0.46374015034212945 1104 84-85 0.6379872643787614 1104 86-87 0.6304533033672577 1104 88-89 0.4193893392654644 1104 90-91 0.5243473148979234 1104 92-93 0.6141673121348212 1104 94-95 0.4747505521472597 1104 96-97 0.7187330830804619 1104 98-99 0.8966953793428694 1104 100-101 1.2975021117645156 1105 1 0.03711683051157877 1105 2 0.024904233661594333 1105 3 0.05091328832133257 1105 4 0.10604077318818383 1105 5 0.14921302550536808 1105 6 0.09055987027537782 1105 7 -0.013612000584394934 1105 8 0.07392176951775298 1105 9 0.03925327852501681 1105 10-11 0.1642416189960656 1105 12-13 0.05288693056095184 1105 14-15 0.18788230076553702 1105 16-17 0.06935780708310091 1105 18-19 0.1679630232674043 1105 20-21 0.10638326030511536 1105 22-23 0.12248065602828717 1105 24-25 0.11648793317252881 1105 26-27 -0.009552477630748513 1105 28-29 0.06147650990827458 1105 30-31 0.07517407741455173 1105 32-33 0.08832337386177613 1105 34-35 0.29248229035985673 1105 36-37 0.26751318040575 1105 38-39 0.20076358675103734 1105 40-41 0.22605286488190046 1105 42-43 0.2971241941555718 1105 44-45 0.24742364912822268 1105 46-47 0.30475625764407965 1105 48-49 0.42763219042178235 1105 50-51 0.34943746444329093 1105 52-53 0.4435506425751825 1105 54-55 0.3281910732808697 1105 56-57 0.1584908896680659 1105 58-59 0.14663855362686462 1105 60-61 0.34812812144846816 1105 62-63 0.2414496020499044 1105 64-65 0.44901362609217443 1105 66-67 0.24218120456650638 1105 68-69 0.32901016974404484 1105 70-71 0.44729813979337507 1105 72-73 0.49339315834800246 1105 74-75 0.33198849931436314 1105 76-77 0.2543023329796874 1105 78-79 0.34233649364996666 1105 80-81 0.46228286174805433 1105 82-83 0.5070951013675362 1105 84-85 0.6911180035890503 1105 86-87 0.8373422687789507 1105 88-89 0.6756068170738523 1105 90-91 0.6685067118982282 1105 92-93 0.5428097975954849 1105 94-95 0.9031969479232025 1105 96-97 0.9347043487676352 1105 98-99 0.7649374592326801 1105 100-101 1.100637633650038 1106 1 0.014573157163532358 1106 2 0.06898614018945892 1106 3 0.021682776518900937 1106 4 0.0792354737689891 1106 5 0.05207278791160519 1106 6 -0.04037252064412655 1106 7 -0.021769838524825502 1106 8 0.05074657309056363 1106 9 0.03438574301024744 1106 10-11 -0.07144088361568635 1106 12-13 -0.004076482670129167 1106 14-15 0.06134165108616685 1106 16-17 -0.08391933161347964 1106 18-19 -0.005374998201020276 1106 20-21 -0.09942550283341944 1106 22-23 -0.18415576345419993 1106 24-25 -0.1229314309038898 1106 26-27 -0.14767605418444418 1106 28-29 -0.24647754216886852 1106 30-31 -0.20500429636312845 1106 32-33 -0.1735721128476868 1106 34-35 -0.10927204422308279 1106 36-37 -0.34911787910857583 1106 38-39 -0.10691564361380301 1106 40-41 -0.11212254779156439 1106 42-43 6.000169831139601E-4 1106 44-45 -0.04354955984880604 1106 46-47 -0.10820824143718966 1106 48-49 -0.3627565237905088 1106 50-51 -0.14050703164831901 1106 52-53 -0.38357185652624537 1106 54-55 -0.3867778712358003 1106 56-57 -0.5173747462387226 1106 58-59 -0.3989830578151299 1106 60-61 -0.32749696672961903 1106 62-63 -0.2285360984032465 1106 64-65 -0.2571717309703061 1106 66-67 -0.22292095184966598 1106 68-69 -0.30975185816726025 1106 70-71 -0.2919646556872024 1106 72-73 -0.20723047069265377 1106 74-75 -0.45249999848370237 1106 76-77 -0.4767382755499021 1106 78-79 -0.4530363820978671 1106 80-81 -0.36093172089244874 1106 82-83 -0.216646670776921 1106 84-85 0.024451599357174558 1106 86-87 0.21993744652918323 1106 88-89 0.09270114747463509 1106 90-91 0.19872397851182555 1106 92-93 0.11058725625256116 1106 94-95 0.16693252240765233 1106 96-97 0.43394208067600815 1106 98-99 0.3553797836239134 1106 100-101 0.7556558697187405 1107 1 -0.015083522582827413 1107 2 0.04279384162843769 1107 3 -0.054806646079768484 1107 4 0.05369902121581305 1107 5 0.020857399059579507 1107 6 0.023439073659112353 1107 7 0.08163551316966533 1107 8 0.008805148302236887 1107 9 0.07633688320852627 1107 10-11 0.09651622028090401 1107 12-13 0.024814801445756984 1107 14-15 0.07718340872219898 1107 16-17 0.12478637923850044 1107 18-19 0.043181057369338305 1107 20-21 6.759394471131941E-4 1107 22-23 -0.005152182599765354 1107 24-25 0.15345579004888066 1107 26-27 0.08040296582326789 1107 28-29 0.08161927825663184 1107 30-31 0.11206508707461893 1107 32-33 0.19132249282169056 1107 34-35 0.27981839909664785 1107 36-37 0.21627548444005384 1107 38-39 0.05241613048929139 1107 40-41 0.07331631961050533 1107 42-43 0.18304296463402636 1107 44-45 0.19397741716453254 1107 46-47 -0.004583678826058701 1107 48-49 0.026474457959487552 1107 50-51 0.12189200904986564 1107 52-53 0.04354710230342107 1107 54-55 0.10382688064937895 1107 56-57 0.11393158778421508 1107 58-59 0.2757332912990833 1107 60-61 0.2729445148994287 1107 62-63 0.2247527688759945 1107 64-65 0.06925694239829028 1107 66-67 0.12580017635287533 1107 68-69 0.28137114941991825 1107 70-71 0.1499686282576036 1107 72-73 0.35743485130171493 1107 74-75 0.2350773758229039 1107 76-77 0.16836689990481446 1107 78-79 0.13449371054694126 1107 80-81 0.33025213642904916 1107 82-83 0.3178952409432796 1107 84-85 0.4953145823721776 1107 86-87 0.4825355972958185 1107 88-89 0.2208235071444733 1107 90-91 0.4676789666824739 1107 92-93 0.8134994001353242 1107 94-95 0.959597186880945 1107 96-97 0.9630717831856899 1107 98-99 0.8017152649934438 1107 100-101 1.5034207112358366 1108 1 0.011044896995670683 1108 2 0.07614125486555423 1108 3 -0.06596661467632003 1108 4 0.08733609228659134 1108 5 0.07226031508002961 1108 6 0.07235917300138794 1108 7 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scores pass #Quality Count 2 2.0 3 13.0 4 29.0 5 55.0 6 130.0 7 303.0 8 754.0 9 1388.0 10 2339.0 11 3507.0 12 5240.0 13 7011.0 14 9009.0 15 11657.0 16 14354.0 17 17669.0 18 16315.0 19 13348.0 20 13170.0 21 14168.0 22 15311.0 23 17244.0 24 20522.0 25 24810.0 26 30581.0 27 37702.0 28 47480.0 29 58871.0 30 72029.0 31 88706.0 32 111554.0 33 142580.0 34 186240.0 35 253129.0 36 359176.0 37 455905.0 38 258402.0 39 12955.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9667396984319 18.927380024879582 14.744295559123447 23.361584717565073 2 16.129316886371257 20.26438581925128 38.794334208364056 24.81196308601341 3 20.627348359967808 22.855820919933027 29.18000542301683 27.336825297082328 4 13.691946060909135 16.453497029253015 36.04532164371865 33.809235266119195 5 14.431382675403203 37.02540266615022 33.816941566336354 14.726273092110217 6 33.59655336542641 35.38958831291008 16.440801529312836 14.57305679235068 7 29.827539164541427 31.761730857122693 19.6846093530115 18.72612062532438 8 26.74993480107658 34.79931211908121 18.820454645218877 19.630298434623338 9 25.9721525284702 17.17989480379643 19.823399140493137 37.024553527240236 10-11 25.915861972803228 26.03511360105489 26.495078019226582 21.5539464069153 12-13 26.688722626402168 23.148644257811576 26.97682725557155 23.185805860214707 14-15 23.164475793523565 25.04543488598781 25.442783029322342 26.34730629116629 16-17 22.90221162302243 28.039634102387378 26.11404745669055 22.94410681789964 18-19 22.793476216646347 27.283709348469483 27.788266675159157 22.134547759725017 20-21 23.635513487785207 26.011056704558072 27.84211359847275 22.511316209183967 22-23 23.14144176246398 25.956337771167505 27.904582261746146 22.99763820462237 24-25 23.528828252694673 25.985644186881203 27.910454120184642 22.575073440239485 26-27 23.13617891339703 26.049297068763615 28.251328634070788 22.563195383768566 28-29 22.787304077967065 26.870607698023303 27.875407799934283 22.46668042407535 30-31 23.206670611652054 26.131561676260155 27.709892947442032 22.95187476464576 32-33 23.091933940348504 26.144835331837996 27.850082990971814 22.913147736841687 34-35 23.54267838176081 26.190818432681535 27.705744332530557 22.560758853027096 36-37 23.446390292459622 26.020845768938948 27.96818091638238 22.564583022219047 38-39 23.147712408174527 26.46742698450509 27.903281649192955 22.481578958127432 40-41 23.320210323912253 26.346542987897507 27.572091197742864 22.761155490447376 42-43 23.13135632155216 26.424738118637492 27.189331203235085 23.25457435657526 44-45 23.45127563777585 26.13280042967402 27.329229969418435 23.086693963131697 46-47 23.812014758673904 26.624163143295434 27.039952867266397 22.523869230764266 48-49 23.7660053127794 26.53564392973532 26.92716284884615 22.771187908639128 50-51 23.221653649456062 26.811042691587755 27.26926485843743 22.698038800518752 52-53 23.714589177218297 27.005655747370305 26.445654129229222 22.834100946182176 54-55 23.349471944638108 26.62266205317565 26.783036813248295 23.244829188937953 56-57 23.640132605740682 26.710966143526516 26.657937620895993 22.99096362983681 58-59 23.993977608671553 26.548134468504593 26.566989417767346 22.890898505056505 60-61 23.605202774818977 26.674089199096123 27.217822342626135 22.502885683458764 62-63 23.271401402286813 26.770188567740433 27.137471583774037 22.820938446198717 64-65 23.179405439061558 27.116213511973804 26.731481658840817 22.972899390123825 66-67 23.504130232510096 26.684176867082858 26.537550815588585 23.274142084818457 68-69 23.405124740169477 26.697193615162483 26.77788632441482 23.119795320253225 70-71 23.75225264480673 26.59391498101592 26.60848253238698 23.045349841790365 72-73 23.65079112330644 26.498176581923843 26.877707476745716 22.973324818023997 74-75 23.28276478232269 27.283218386550416 26.444588520929596 22.989428310197294 76-77 23.590398519538645 26.76512738853503 26.366500258220004 23.277973833706316 78-79 23.573226570968473 26.845951574309456 26.45863385175833 23.12218800296374 80-81 23.80811566692385 26.78955631671095 26.330409592710126 23.071918423655074 82-83 23.660769628099175 26.796078684573004 26.649513601928376 22.89363808539945 84-85 23.47789513380618 26.99497415615481 26.463847782948292 23.063282927090718 86-87 23.413552141507964 27.060876324501283 26.33955481563562 23.186016718355134 88-89 23.3802601624266 26.937112164773126 26.122804845888474 23.559822826911795 90-91 23.528527827072214 26.637016088943984 26.392171696349898 23.442284387633904 92-93 23.73191240681089 26.846018819168734 26.465158277539153 22.956910496481225 94-95 23.460875401365 26.76831458879581 26.54709969995968 23.223710309879504 96-97 23.05083716708377 26.802566014857327 26.895897958797015 23.250698859261888 98-99 23.113399393824693 27.0623234078963 26.2032485088376 23.62102868944141 100-101 23.032644560833244 26.80937357618227 26.281920432435786 23.876061430548702 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 116.0 1 88.5 2 85.0 3 117.0 4 106.0 5 103.0 6 170.5 7 373.0 8 649.5 9 827.5 10 819.0 11 759.0 12 824.0 13 993.5 14 1144.5 15 1279.0 16 1441.0 17 1606.0 18 1862.5 19 2101.5 20 2335.0 21 2729.0 22 3275.5 23 3945.5 24 4899.5 25 6345.0 26 8208.5 27 10562.5 28 13366.5 29 17091.0 30 21231.5 31 25479.5 32 30360.5 33 34389.5 34 38325.0 35 42337.5 36 47531.0 37 52620.5 38 55868.0 39 59297.0 40 63208.0 41 67525.5 42 70806.0 43 74480.0 44 78322.0 45 82235.5 46 86187.5 47 94813.0 48 109775.0 49 108809.5 50 95009.0 51 88857.5 52 87643.0 53 86850.0 54 85673.5 55 81404.5 56 73917.0 57 66611.0 58 61638.0 59 55090.0 60 45893.5 61 38135.5 62 31009.5 63 24129.0 64 17432.5 65 12328.5 66 9352.0 67 7530.0 68 5989.0 69 4750.5 70 3708.0 71 2547.5 72 1792.5 73 1407.0 74 1062.0 75 740.5 76 506.5 77 313.5 78 198.5 79 129.5 80 83.0 81 57.0 82 43.0 83 27.0 84 12.0 85 8.5 86 4.0 87 2.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0201406575322186 2 0.013384069428461503 3 0.009812115208003932 4 0.0 5 0.0035719542204575715 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.898186394039054E-4 14-15 1.2910677905268332E-4 16-17 1.0758898254390275E-4 18-19 0.0015923169416497609 20-21 0.0 22-23 8.607118603512221E-5 24-25 0.0 26-27 0.0013341033835443943 28-29 0.0026466889705800078 30-31 0.0014201745695795165 32-33 0.009145063516231735 34-35 0.001979637278807811 36-37 9.037474533687832E-4 38-39 0.0010543720289302471 40-41 1.7214237207024443E-4 42-43 0.005788287260861969 44-45 0.0015492813486322 46-47 3.227669476317083E-4 48-49 6.670516917721971E-4 50-51 1.5062457556146386E-4 52-53 1.7214237207024443E-4 54-55 0.0016353525346673218 56-57 0.01635352534667322 58-59 0.02842500918809911 60-61 0.023734129549184947 62-63 0.016418078736199562 64-65 0.02072163803795567 66-67 0.0025821355810536663 68-69 0.0012049966044917109 70-71 2.1517796508780553E-5 72-73 0.0 74-75 0.0020011550753165916 76-77 0.002496064395018544 78-79 0.010436131306758568 80-81 0.01874200075914786 82-83 0.019710301602042988 84-85 0.03290071086192546 86-87 0.012200590620478572 88-89 0.046069602325299164 90-91 0.05002887688291478 92-93 0.03272856848985522 94-95 0.03457909898961035 96-97 0.056032342108864555 98-99 0.059927063276953844 100-101 0.06696338273532508 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2323658.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.89152676120195 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84364809919701 47.40018581421842 2 13.21417400411622 16.09262459997644 3 4.0240383621472855 7.350895188503842 4 1.7608558162785954 4.2888479623818485 5 0.9002561220471871 2.740898487378609 6 0.5165320240276775 1.8871454138459482 7 0.3732698461378026 1.591027957767482 8 0.2360441315821417 1.1498470044046922 9 0.18251883238195224 1.0002465329768082 >10 0.8777073381787672 9.51840549859092 >50 0.04808221095156451 1.9871873831395503 >100 0.020194877886820204 2.162941222412846 >500 0.0014096500352614432 0.5899919784521387 >1k 0.001198202529970616 1.8054260971499962 >5k 0.0 0.0 >10k+ 7.048250176297742E-5 0.4343288588004365 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10120 0.4355202013377184 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4498 0.19357409739298986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4218 0.18152413134807274 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3828 0.16474025007122392 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3633 0.15634830943279948 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2855 0.12286661806513695 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2725 0.11727199097285401 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2566 0.11042933168306178 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2565 0.11038629609004423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2347 0.10100453681221591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.3035593017561106E-5 0.0 0.0 0.0 3 0.0 4.3035593017561106E-5 0.0 0.0 0.0 4 0.0 4.3035593017561106E-5 0.0 0.0 0.0 5 0.0 4.3035593017561106E-5 0.0 0.0 0.0 6 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 0.0 7 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 0.0 8 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 0.0 9 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 0.0 10-11 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 0.0 12-13 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 2.1517796508780553E-5 14-15 8.607118603512221E-5 4.3035593017561106E-5 0.0 0.0 4.3035593017561106E-5 16-17 1.7214237207024443E-4 4.3035593017561106E-5 0.0 0.0 4.3035593017561106E-5 18-19 1.7214237207024443E-4 4.3035593017561106E-5 0.0 0.0 1.2910677905268332E-4 20-21 1.7214237207024443E-4 4.3035593017561106E-5 0.0 4.3035593017561106E-5 1.7214237207024443E-4 22-23 1.7214237207024443E-4 4.3035593017561106E-5 0.0 8.607118603512221E-5 1.7214237207024443E-4 24-25 1.7214237207024443E-4 4.3035593017561106E-5 0.0 1.5062457556146386E-4 1.7214237207024443E-4 26-27 1.7214237207024443E-4 4.3035593017561106E-5 0.0 2.797313546141472E-4 2.1517796508780553E-4 28-29 1.7214237207024443E-4 6.455338952634166E-5 0.0 8.391940638424416E-4 2.1517796508780553E-4 30-31 1.7214237207024443E-4 8.607118603512221E-5 0.0 0.0017429415172112248 2.1517796508780553E-4 32-33 1.7214237207024443E-4 8.607118603512221E-5 0.0 0.003464365237913669 2.1517796508780553E-4 34-35 1.7214237207024443E-4 8.607118603512221E-5 0.0 0.005400966923703919 2.1517796508780553E-4 36-37 1.7214237207024443E-4 8.607118603512221E-5 0.0 0.00740212199902051 2.1517796508780553E-4 38-39 1.7214237207024443E-4 8.607118603512221E-5 0.0 0.009984257580074176 2.1517796508780553E-4 40-41 1.93660168579025E-4 8.607118603512221E-5 0.0 0.013835943155145894 2.1517796508780553E-4 42-43 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.018720482962639083 2.1517796508780553E-4 44-45 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.023561987177114704 2.1517796508780553E-4 46-47 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.03365383373973278 2.1517796508780553E-4 48-49 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.04477853453477233 2.1517796508780553E-4 50-51 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.050911106539774785 2.1517796508780553E-4 52-53 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.06065866835825238 3.012491511229277E-4 54-55 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.07841085047799633 3.012491511229277E-4 56-57 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.10481318679427007 3.4428474414048885E-4 58-59 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.13551908241229993 3.8732033715804993E-4 60-61 2.1517796508780553E-4 8.607118603512221E-5 0.0 0.1715829093610161 3.8732033715804993E-4 62-63 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.20586075919950353 3.8732033715804993E-4 64-65 2.5821355810536664E-4 8.607118603512221E-5 0.0 0.24427002596767683 4.30355930175611E-4 66-67 3.012491511229277E-4 8.607118603512221E-5 0.0 0.30064665282068187 4.7339152319317214E-4 68-69 3.012491511229277E-4 8.607118603512221E-5 0.0 0.3725160931600089 4.7339152319317214E-4 70-71 3.4428474414048885E-4 8.607118603512221E-5 0.0 0.4122809811082354 4.7339152319317214E-4 72-73 3.8732033715804993E-4 8.607118603512221E-5 0.0 0.4691525172809424 4.7339152319317214E-4 74-75 3.8732033715804993E-4 8.607118603512221E-5 0.0 0.5293162763194927 4.949093197019527E-4 76-77 4.30355930175611E-4 8.607118603512221E-5 0.0 0.5920406531425881 5.164271162107333E-4 78-79 4.7339152319317214E-4 8.607118603512221E-5 0.0 0.6689667756614786 5.164271162107333E-4 80-81 4.7339152319317214E-4 8.607118603512221E-5 0.0 0.7820427963151204 5.164271162107333E-4 82-83 4.949093197019527E-4 8.607118603512221E-5 0.0 0.9096002940191714 6.024983022458554E-4 84-85 5.164271162107333E-4 8.607118603512221E-5 0.0 1.0963317321223691 6.455338952634166E-4 86-87 5.164271162107333E-4 8.607118603512221E-5 0.0 1.3495531614376985 6.885694882809777E-4 88-89 5.164271162107333E-4 8.607118603512221E-5 0.0 1.632791916882777 7.316050812985387E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7400 0.0 23.303822 1 TACATGG 7445 0.0 22.075348 2 GAGTACT 6775 0.0 20.363771 12-13 ACATGGG 7680 0.0 20.283524 3 GTACTTT 7080 0.0 19.45256 14-15 GTATAGC 810 0.0 18.767967 1 CATGGGG 5100 0.0 18.344595 4 AGAGTAC 10585 0.0 18.01347 10-11 ACGGTCC 325 1.006083E-8 17.53479 8 AGTACTT 7310 0.0 17.411604 12-13 TTACGCG 100 2.7597576E-4 16.627245 80-81 TACGCCC 200 2.765815E-4 16.621878 4 GAGTACA 6155 0.0 16.594442 1 ACTTTTT 8335 0.0 16.523767 16-17 GTACCTG 1825 0.0 16.139166 1 TACTTTT 8060 0.0 15.879462 14-15 CGGTCCG 180 0.0026233494 15.83002 9 GTGTAGC 1270 0.0 15.710783 1 AGTACAT 5830 0.0 15.643316 2 TACACTG 1890 0.0 15.076533 5 >>END_MODULE