FastQCFastQC Report
Thu 26 May 2016
SRR936571_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936571_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831279
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65850.3595847492381008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56990.31120326285617866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30690.16758778973602603No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29020.15846848022611518No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18870.10304273679761522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17050.031.4850122
GTACCTG18600.029.372081
GGTATCA78400.028.2976671
GTACATG82650.026.3252451
GTATCAA107800.025.9565141
TACATGG83350.025.47722
TATCACG950.001225907425.0032352
ACATGGG84200.024.0909393
ATCAACG125850.022.1953533
TCAACGC126800.022.0296654
GAGTACT64950.021.9068212-13
CAACGCA128200.021.7149775
TATCAAC128850.021.6054342
TAGCGCG1100.002874938521.5948839
AACGCAG130250.021.4832136
GTACTTT69300.020.39488814-15
CATGGGA50000.019.8575694
ACGCAGA144900.019.113937
CATGGGG38450.019.0271684
CGCAGAG145750.019.0024598
Produced by FastQC (version 0.11.5)