##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936571_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1831279 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.169268582231325 31.0 31.0 34.0 28.0 34.0 2 31.235338252663848 31.0 31.0 34.0 28.0 34.0 3 31.23959702481162 31.0 31.0 34.0 27.0 34.0 4 34.87478805796386 37.0 35.0 37.0 32.0 37.0 5 34.88540686591175 37.0 35.0 37.0 32.0 37.0 6 34.86263152692735 37.0 35.0 37.0 32.0 37.0 7 34.762970579578536 37.0 35.0 37.0 32.0 37.0 8 34.71108935339727 37.0 35.0 37.0 31.0 37.0 9 36.17773971087966 39.0 35.0 39.0 32.0 39.0 10-11 36.029115170326314 39.0 35.0 39.0 30.0 39.0 12-13 35.91426292771337 39.0 35.0 39.0 30.0 39.0 14-15 36.95763097813058 39.0 36.0 41.0 31.0 41.0 16-17 36.837692945749936 39.0 36.0 41.0 30.5 41.0 18-19 36.80044138550161 39.0 36.0 41.0 30.0 41.0 20-21 36.66781003877618 39.0 36.0 41.0 30.0 41.0 22-23 36.77217234512054 39.0 36.0 41.0 30.0 41.0 24-25 36.812175261115314 39.0 36.0 41.0 30.0 41.0 26-27 36.70985797358021 39.0 36.0 41.0 30.0 41.0 28-29 36.561068247929455 39.0 36.0 41.0 30.0 41.0 30-31 36.32984487890704 39.0 35.5 41.0 29.5 41.0 32-33 36.13416005971783 39.0 35.0 40.0 29.0 41.0 34-35 35.94118837162442 38.5 35.0 40.0 28.5 41.0 36-37 35.72139635740922 38.0 35.0 40.0 27.0 41.0 38-39 35.51038618364542 38.0 35.0 40.0 27.0 41.0 40-41 35.29381568837954 38.0 34.0 40.0 26.0 41.0 42-43 35.04193435298499 38.0 34.0 40.0 25.0 41.0 44-45 34.75461685521431 38.0 33.5 40.0 24.5 41.0 46-47 34.553536899620426 38.0 33.0 40.0 24.0 41.0 48-49 34.370326968200914 38.0 33.0 40.0 23.0 41.0 50-51 33.40413121102792 37.0 32.0 39.5 22.0 40.5 52-53 33.50074674585358 37.5 33.0 39.5 22.0 40.5 54-55 33.923899635173015 38.0 33.0 40.0 22.0 41.0 56-57 33.8085166159826 38.0 33.0 40.0 21.5 41.0 58-59 33.55684524313335 37.5 33.0 40.0 20.5 41.0 60-61 33.248388694458896 37.0 32.5 40.0 19.5 41.0 62-63 32.86848508610649 36.5 32.0 40.0 18.0 41.0 64-65 32.49764672668665 36.0 31.5 39.0 15.5 41.0 66-67 32.037356951070805 35.5 31.0 39.0 13.0 40.5 68-69 31.638747836894325 35.0 31.0 38.5 9.5 40.0 70-71 31.2411443040629 35.0 30.5 38.0 8.0 40.0 72-73 30.836745247447276 35.0 30.0 37.0 7.0 39.5 74-75 30.395157428223662 35.0 29.5 37.0 4.0 39.0 76-77 29.919878674958866 34.0 29.0 36.0 2.0 39.0 78-79 29.40901877867873 34.0 29.0 36.0 2.0 38.0 80-81 28.937551569149214 34.0 28.5 35.0 2.0 37.0 82-83 28.5145510323659 34.0 27.5 35.0 2.0 37.0 84-85 28.12632837486806 34.0 27.0 35.0 2.0 36.0 86-87 27.73991838491022 33.5 26.0 35.0 2.0 36.0 88-89 27.318573521566076 33.0 26.0 35.0 2.0 35.5 90-91 26.881904941846656 33.0 25.0 35.0 2.0 35.0 92-93 26.40170339964582 33.0 24.5 35.0 2.0 35.0 94-95 25.870765186517183 32.5 23.5 35.0 2.0 35.0 96-97 25.23460188207258 32.0 19.0 35.0 2.0 35.0 98-99 24.41656214044938 32.0 8.0 34.5 2.0 35.0 100-101 21.511328421283704 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5438993705554545 1101 2 0.5228050749600932 1101 3 0.5227161425754261 1101 4 0.5916556715062029 1101 5 0.47825363901546325 1101 6 0.694947048325794 1101 7 0.644579880455801 1101 8 0.693195464008248 1101 9 0.71395611277417 1101 10-11 0.8414422922375806 1101 12-13 0.8644718914167342 1101 14-15 1.0085766640804366 1101 16-17 1.021320667541879 1101 18-19 0.8813246875443994 1101 20-21 0.9451430958774694 1101 22-23 0.932948986946883 1101 24-25 0.9992627414846496 1101 26-27 0.8193802571478912 1101 28-29 1.0224456978764422 1101 30-31 0.9599780204969548 1101 32-33 0.8443591805514501 1101 34-35 0.7733031132371977 1101 36-37 0.6592823422671756 1101 38-39 0.6862678366135313 1101 40-41 0.7772821990121344 1101 42-43 0.7682507958652351 1101 44-45 0.6455529262581621 1101 46-47 0.7990468033541944 1101 48-49 0.9627432803695655 1101 50-51 0.8455352383768329 1101 52-53 0.942783434778903 1101 54-55 0.8457533783313451 1101 56-57 0.6988915417613626 1101 58-59 0.8952752698520854 1101 60-61 0.9199057895681619 1101 62-63 0.8679467424791127 1101 64-65 0.5857840726582637 1101 66-67 0.8527688283818442 1101 68-69 0.7772497716002782 1101 70-71 0.7579300645178648 1101 72-73 0.84090458466477 1101 74-75 0.7374770063676728 1101 76-77 0.7226601307722973 1101 78-79 0.7268763812402241 1101 80-81 0.5858533111477335 1101 82-83 0.46589090276980727 1101 84-85 0.4579331277679408 1101 86-87 0.46350150467636553 1101 88-89 0.4378379580530378 1101 90-91 0.4452070122459659 1101 92-93 0.3647121621556799 1101 94-95 0.45174355517046294 1101 96-97 0.3863698810430378 1101 98-99 0.32220546928270366 1101 100-101 0.5927917683952373 1102 1 0.387981355393606 1102 2 0.5728957826768344 1102 3 0.5709035115012995 1102 4 0.6171639000621312 1102 5 0.5365667604812572 1102 6 0.5484774260747898 1102 7 0.5189428135286391 1102 8 0.6561804751231364 1102 9 0.6935426998250733 1102 10-11 0.7631961991555443 1102 12-13 0.7550070620317584 1102 14-15 0.8089192011841106 1102 16-17 0.6853507828969327 1102 18-19 0.786969867063128 1102 20-21 0.7156445876901927 1102 22-23 0.5868542096672513 1102 24-25 0.7500101219676552 1102 26-27 0.5040478521846197 1102 28-29 0.45189968754186083 1102 30-31 0.7705439708052424 1102 32-33 0.5138220542662637 1102 34-35 0.5594628649555276 1102 36-37 0.3463966133525389 1102 38-39 0.3920662512431292 1102 40-41 0.25442358413764055 1102 42-43 0.418090878062344 1102 44-45 0.5619111735845905 1102 46-47 0.6464551472848399 1102 48-49 0.7051435608460821 1102 50-51 0.7306592711063047 1102 52-53 0.8832311898857554 1102 54-55 0.9466525689394132 1102 56-57 0.863239699205586 1102 58-59 0.903033457623934 1102 60-61 0.8255875764807001 1102 62-63 0.8690366109283545 1102 64-65 1.0643058513550159 1102 66-67 0.9303026742514398 1102 68-69 1.2000548512324123 1102 70-71 1.0704309244230892 1102 72-73 0.6286035667335348 1102 74-75 0.7533251824841223 1102 76-77 0.9175981881137254 1102 78-79 0.9570493008100982 1102 80-81 1.118598133535535 1102 82-83 1.1090177048854706 1102 84-85 0.6611335230295197 1102 86-87 0.7793293378921007 1102 88-89 0.7404560500658484 1102 90-91 0.7613178771239006 1102 92-93 0.7703252794939246 1102 94-95 0.8000895643257486 1102 96-97 0.8880264270346991 1102 98-99 1.0009604788448492 1102 100-101 1.119062364353784 1103 1 0.34676834298687 1103 2 0.33111497805524337 1103 3 0.2914234308487771 1103 4 0.4061930589021472 1103 5 0.46279966406924444 1103 6 0.3894905068574559 1103 7 0.42981177065964715 1103 8 0.36680336629856214 1103 9 0.09171184673815702 1103 10-11 0.4192435116122013 1103 12-13 0.10981637184273296 1103 14-15 0.5589986007967838 1103 16-17 0.544763934775041 1103 18-19 0.6798606394198217 1103 20-21 0.741352701116547 1103 22-23 0.514693437835021 1103 24-25 0.501546152394944 1103 26-27 0.48892526112516776 1103 28-29 0.4899904983600223 1103 30-31 0.5429466807844392 1103 32-33 0.5308726961647778 1103 34-35 0.6857563259239399 1103 36-37 0.6873197321887545 1103 38-39 0.7895849040817993 1103 40-41 0.5765226816529889 1103 42-43 0.6143440176250081 1103 44-45 0.7602431504067084 1103 46-47 0.9859953288307182 1103 48-49 0.991939453822205 1103 50-51 1.087691891478059 1103 52-53 1.1590820769275538 1103 54-55 1.266676835597785 1103 56-57 1.2862875128267746 1103 58-59 1.1019682159091744 1103 60-61 1.2518589658136605 1103 62-63 1.3220378677246671 1103 64-65 1.2539453916822438 1103 66-67 1.2360218987673228 1103 68-69 1.1364244387040792 1103 70-71 1.2441616475568829 1103 72-73 1.0796125488450699 1103 74-75 1.0198744170427183 1103 76-77 1.1266362435953603 1103 78-79 1.1207665672878235 1103 80-81 1.1474397393210722 1103 82-83 0.672566289678425 1103 84-85 0.7934784728256545 1103 86-87 0.9898205034104244 1103 88-89 0.7972962174314873 1103 90-91 1.0418603828688404 1103 92-93 0.8802422668346672 1103 94-95 0.8030743341688549 1103 96-97 1.0293852967727162 1103 98-99 1.0870297803807851 1103 100-101 1.1449221140434673 1104 1 0.36555772317027646 1104 2 0.4121016443240286 1104 3 0.3907932516522479 1104 4 0.5484338842910574 1104 5 0.38117598400178565 1104 6 0.46173898988863016 1104 7 0.40586009996040673 1104 8 0.49692263973222595 1104 9 0.5445793233212228 1104 10-11 0.6318221396187624 1104 12-13 0.6555482831295976 1104 14-15 0.4441143534477163 1104 16-17 0.5540497284769046 1104 18-19 0.6123875939836765 1104 20-21 0.31684172411979006 1104 22-23 0.5026232657233791 1104 24-25 0.595394625414869 1104 26-27 0.30308713434521906 1104 28-29 0.47434323025157 1104 30-31 0.6901078180814935 1104 32-33 0.593833941564057 1104 34-35 0.5248776126773791 1104 36-37 0.2798914100787613 1104 38-39 0.33047844648977787 1104 40-41 0.14304309425118333 1104 42-43 0.30120083704182576 1104 44-45 0.3673419676836147 1104 46-47 0.18097894744452248 1104 48-49 0.37773626013775896 1104 50-51 0.43447972473109076 1104 52-53 0.5532920618962365 1104 54-55 0.3774493718560805 1104 56-57 0.33114620742738765 1104 58-59 0.39719968511279546 1104 60-61 0.3397532730232484 1104 62-63 0.5166111695546576 1104 64-65 0.5727267580539817 1104 66-67 0.5543246023337822 1104 68-69 0.505220172179623 1104 70-71 0.5617047235995329 1104 72-73 0.25928665865016853 1104 74-75 0.31304263055953996 1104 76-77 0.18396411630618914 1104 78-79 0.39449178162720244 1104 80-81 0.5984332353789021 1104 82-83 0.2733647589200707 1104 84-85 0.2696577184492206 1104 86-87 0.27676285161607694 1104 88-89 0.2718551329052694 1104 90-91 0.23015403299400106 1104 92-93 0.012162963309503283 1104 94-95 0.21545942742026014 1104 96-97 0.20649318193022026 1104 98-99 0.306253374390014 1104 100-101 0.3413435259856321 1105 1 0.3962786703773027 1105 2 0.27225908831598744 1105 3 0.3541596531851887 1105 4 0.31226666424255 1105 5 0.32782732978665763 1105 6 0.4250975059151827 1105 7 0.5294972010708321 1105 8 0.5870697487823691 1105 9 0.6192097271725032 1105 10-11 0.6409578684046551 1105 12-13 0.37909918579830304 1105 14-15 0.3418690294297875 1105 16-17 0.4301663285028283 1105 18-19 0.38492067491508664 1105 20-21 0.34053705476647167 1105 22-23 0.2716120579545773 1105 24-25 0.3738592088950341 1105 26-27 0.15475878777326812 1105 28-29 0.18198918974274392 1105 30-31 -0.10201335356676822 1105 32-33 -0.2505181137685426 1105 34-35 -0.009992712634264933 1105 36-37 0.1325700673150294 1105 38-39 0.21577192778806875 1105 40-41 0.11615277819515768 1105 42-43 0.012965125456197768 1105 44-45 0.22706583203516573 1105 46-47 0.45659476853025893 1105 48-49 0.36220341429299907 1105 50-51 0.45254307886080625 1105 52-53 0.5255949599676839 1105 54-55 0.35533200496465867 1105 56-57 0.2766503352435663 1105 58-59 0.4714473954820875 1105 60-61 0.620187384935349 1105 62-63 0.246957776988026 1105 64-65 0.28553501358776145 1105 66-67 0.5057633129746328 1105 68-69 0.38899852735170626 1105 70-71 0.3157129018553455 1105 72-73 0.2824472533947784 1105 74-75 0.3946427131602199 1105 76-77 0.1747045604277666 1105 78-79 0.19773570646058403 1105 80-81 0.41495277985291423 1105 82-83 0.30693372925528806 1105 84-85 0.3692144544212752 1105 86-87 0.10604755240299824 1105 88-89 0.1751076085846428 1105 90-91 -0.037437053229574246 1105 92-93 -0.21833938287507237 1105 94-95 0.014110291203561331 1105 96-97 0.20872404097235986 1105 98-99 0.6523828721473279 1105 100-101 0.7573352836600797 1106 1 0.20902827994552453 1106 2 0.07807343189544014 1106 3 0.31258848866083255 1106 4 0.25945787128130604 1106 5 0.15040688945791914 1106 6 0.33695422109868645 1106 7 0.4533359432138937 1106 8 0.4152331930060882 1106 9 0.5130537381933848 1106 10-11 0.45473156971839757 1106 12-13 0.48660335752635575 1106 14-15 0.5397664426641171 1106 16-17 0.4749290153285628 1106 18-19 0.3575635813908491 1106 20-21 0.18151827898345374 1106 22-23 0.4235521386075618 1106 24-25 0.34703463284265723 1106 26-27 0.2920866248234333 1106 28-29 0.3445238347255497 1106 30-31 0.4386893033653152 1106 32-33 0.4184882434306658 1106 34-35 0.4083288056009451 1106 36-37 0.30761746546433955 1106 38-39 0.23776597413526446 1106 40-41 0.3953752822146299 1106 42-43 0.5094171414352999 1106 44-45 0.3665623791161323 1106 46-47 0.1857120101460552 1106 48-49 0.45093346631900744 1106 50-51 0.12914171243274097 1106 52-53 0.0989476423741138 1106 54-55 0.233463423930516 1106 56-57 0.2624714498044227 1106 58-59 0.379034246881055 1106 60-61 0.3914260932490947 1106 62-63 0.4221516750184833 1106 64-65 0.38690499858975613 1106 66-67 0.6337795152217502 1106 68-69 0.6203919548936305 1106 70-71 0.23743129812050867 1106 72-73 0.15961202904012595 1106 74-75 0.3806305433063386 1106 76-77 0.4070488937483887 1106 78-79 0.19625049194509359 1106 80-81 0.37340144719019364 1106 82-83 0.31037807580568355 1106 84-85 0.4131384454209375 1106 86-87 0.23501824295865958 1106 88-89 0.11550505956347834 1106 90-91 -0.24213154915866753 1106 92-93 -0.04974987861275437 1106 94-95 0.20054680762319066 1106 96-97 0.043369049369552215 1106 98-99 0.2864598192586989 1106 100-101 0.33036502659053113 1107 1 0.26730277937509683 1107 2 0.3402136081491385 1107 3 0.24490314252973633 1107 4 0.30363756834425715 1107 5 0.3240759329485101 1107 6 0.28841725054382295 1107 7 0.3897105231272917 1107 8 0.4095753244459317 1107 9 0.5050464247795787 1107 10-11 0.5669911649738566 1107 12-13 0.4878750178878377 1107 14-15 0.2473553628848606 1107 16-17 0.5090694358605177 1107 18-19 0.4414246286618848 1107 20-21 0.5226400708978431 1107 22-23 0.4801962939834681 1107 24-25 0.45875060229401043 1107 26-27 0.353440603758969 1107 28-29 0.391936885265018 1107 30-31 0.5754888634511062 1107 32-33 0.304695242056205 1107 34-35 0.4028261444079604 1107 36-37 0.4299207374596534 1107 38-39 0.2385170764542508 1107 40-41 0.20408513564234454 1107 42-43 0.2820188809312043 1107 44-45 0.1838894415740242 1107 46-47 0.24619821455788582 1107 48-49 0.4627206924860161 1107 50-51 0.17912555166564914 1107 52-53 0.2109937608298722 1107 54-55 0.37498787495779595 1107 56-57 0.2472968628506962 1107 58-59 0.2693027716270322 1107 60-61 0.32191159362750454 1107 62-63 0.35748033861904815 1107 64-65 0.12236964197816036 1107 66-67 0.11887694117956826 1107 68-69 0.32727230814773023 1107 70-71 0.12914922499810544 1107 72-73 0.06172591604596178 1107 74-75 0.17456089520037565 1107 76-77 0.10231509571744013 1107 78-79 0.21611890169894465 1107 80-81 0.32421297904423696 1107 82-83 0.40028914358437007 1107 84-85 0.3019353003116514 1107 86-87 0.2853466318795199 1107 88-89 0.05593036508464877 1107 90-91 0.1589321546651412 1107 92-93 0.13381150110073392 1107 94-95 0.10277243326622099 1107 96-97 0.032223053639622634 1107 98-99 0.05113931604547517 1107 100-101 -0.12239273097769043 1108 1 0.4205711651093331 1108 2 0.4051044348684911 1108 3 0.4542481687750559 1108 4 0.4530831586139854 1108 5 0.33868090159367625 1108 6 0.31491694781885826 1108 7 0.3193662517714415 1108 8 0.1819585665588761 1108 9 0.4922396753864007 1108 10-11 0.4933287313288588 1108 12-13 0.2118121016410086 1108 14-15 0.4508230746027948 1108 16-17 0.5126655996594494 1108 18-19 0.5506745520191814 1108 20-21 0.5764361100905617 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2315 86-87 -1.1850729214914182 2315 88-89 -1.2434034680022314 2315 90-91 -0.9446280759060848 2315 92-93 -0.837990361451066 2315 94-95 -1.1077210790655947 2315 96-97 -0.9822315760407179 2315 98-99 -1.3695410676009452 2315 100-101 -1.2323892851884004 2316 1 -4.188962359615253 2316 2 -2.761174372934331 2316 3 -2.2765962391979286 2316 4 -1.187844908565097 2316 5 -1.1582695082697256 2316 6 -4.073440741540807 2316 7 -5.405211582000092 2316 8 -6.137204388283784 2316 9 -5.713293008592743 2316 10-11 -5.775935415031334 2316 12-13 -3.8440807939341255 2316 14-15 -2.6103886686476443 2316 16-17 -2.7302478476108334 2316 18-19 -3.1061166070642017 2316 20-21 -3.522713956678224 2316 22-23 -4.101394879791364 2316 24-25 -3.2773429211637577 2316 26-27 -3.5423262461775877 2316 28-29 -2.7308345127250107 2316 30-31 -2.3997976068650004 2316 32-33 -2.482150254353847 2316 34-35 -2.179147651263264 2316 36-37 -2.561196791336215 2316 38-39 -2.152187009848383 2316 40-41 -2.2191306494643044 2316 42-43 -2.3049807804763205 2316 44-45 -2.695171342596211 2316 46-47 -2.379565323869876 2316 48-49 -2.5022470792158416 2316 50-51 -2.5928832691023516 2316 52-53 -2.3502576794284025 2316 54-55 -2.298815795979934 2316 56-57 -2.5199192620886848 2316 58-59 -2.2425650185547816 2316 60-61 -2.9220253686398934 2316 62-63 -2.655145563829116 2316 64-65 -2.595078660656533 2316 66-67 -2.593984859760301 2316 68-69 -2.4555017068024263 2316 70-71 -2.5229618928655633 2316 72-73 -2.141463364587665 2316 74-75 -2.0241510270994745 2316 76-77 -2.245722308208496 2316 78-79 -3.019552931500236 2316 80-81 -3.2300362324569925 2316 82-83 -2.9764396314426413 2316 84-85 -2.8118206080029644 2316 86-87 -2.661675118655964 2316 88-89 -3.0214231316577944 2316 90-91 -3.1524510637989422 2316 92-93 -4.016320153078528 2316 94-95 -3.916470595511658 2316 96-97 -4.255845344520317 2316 98-99 -5.208969467638585 2316 100-101 -5.576763984600817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5526.0 3 2312.0 4 2401.0 5 2621.0 6 2936.0 7 3451.0 8 4242.0 9 5379.0 10 6396.0 11 7909.0 12 9365.0 13 11366.0 14 12494.0 15 14143.0 16 16534.0 17 20076.0 18 17317.0 19 13901.0 20 14467.0 21 15400.0 22 17208.0 23 19095.0 24 21686.0 25 24985.0 26 28321.0 27 33530.0 28 39109.0 29 46250.0 30 55648.0 31 66950.0 32 82388.0 33 103171.0 34 133475.0 35 176746.0 36 240741.0 37 302187.0 38 221461.0 39 30083.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.87887224869774 18.20744690728012 13.807220151148908 23.106460692873238 2 15.728007724521204 19.7100355424095 39.920603019466405 24.64135371360289 3 19.71737832735026 24.102143146992432 29.390840737761188 26.789637787896115 4 12.743769027174109 15.96491189742503 36.70208756842136 34.589231506979495 5 13.808855588286864 37.125006349922515 33.916943559703746 15.149194502086871 6 32.46415877327454 35.18875851300839 17.390957554328644 14.956125159388428 7 29.170666815204605 32.440849680698044 20.235945962771588 18.152537541325767 8 27.002628296020852 34.125572686695314 19.42983673103771 19.441962286246124 9 26.76813390485357 16.47541641789659 20.202952684613802 36.55349699263604 10-11 25.39269618420693 26.226571249078145 26.847614105050393 21.53311846166453 12-13 26.23620179727249 23.61919650672673 27.53189526407255 22.612706431928228 14-15 23.005710065861845 25.02990492691181 26.079117239330085 25.885267767896252 16-17 22.284009893310436 28.43856992722037 26.696589123913277 22.58083105555592 18-19 22.1521746017354 26.85430774659196 29.087571784852457 21.905945866820186 20-21 22.954950003332748 25.894159606888845 29.096874757556318 22.05401563222209 22-23 22.563940403300638 25.86897289574619 29.158604313065627 22.408482387887545 24-25 22.5021713601083 26.070737874718326 29.220732236417636 22.206358528755743 26-27 22.29567808149423 26.193625858937992 29.25843074585046 22.252265313717317 28-29 21.954521886782253 26.506046465402683 29.285637641284644 22.253794006530416 30-31 22.134266695827417 26.117275916140446 29.238541230654725 22.50991615737741 32-33 21.978501446697717 26.346608403761497 29.180317484414246 22.494572665126544 34-35 22.664132554351358 26.238273905833026 28.874054690738006 22.223538849077613 36-37 22.545731153757206 26.09814973022872 28.913458311934292 22.442660804079782 38-39 22.28024784863475 26.326654758777877 29.090078573499724 22.303018819087644 40-41 22.436141244620813 26.193577769483493 29.060528923300144 22.309752062595546 42-43 22.27252621231979 26.26485364788117 28.70243556138052 22.760184578418524 44-45 22.433312136362506 26.38695220415277 28.545794057730937 22.633941601753786 46-47 22.624404985209612 26.64314730264512 28.114786728460288 22.617660983684974 48-49 22.707317466077235 26.747742191081475 27.91653048960126 22.62840985324003 50-51 22.469862905229586 26.929961801317702 27.967251251218457 22.632924042234254 52-53 22.758897310444688 26.967663792343167 27.633943284057267 22.639495613154885 54-55 22.46667034371882 26.832796611141358 27.718811452961674 22.981721592178143 56-57 22.647029913829822 27.208888961698502 27.49074407784804 22.653337046623637 58-59 22.86660512176961 26.79848851849091 27.54322546047406 22.791680899265423 60-61 22.66415323899606 26.738871283852227 28.143500191947386 22.453475285204323 62-63 22.506624510072943 26.889272047912172 28.05676942336387 22.547334018651018 64-65 22.38867056688394 27.064617337027318 27.79220162232074 22.754510473768004 66-67 22.369575891235733 27.202699772634055 27.47911078778505 22.948613548345165 68-69 22.287534050846897 26.974635004147697 27.843889312110754 22.893941632894656 70-71 22.460113041390326 26.92680773484798 27.697817652671574 22.91526157109012 72-73 22.533515161080853 26.883290725419528 27.705147411002617 22.878046702497002 74-75 22.251872632325536 27.371497972037577 27.439365275482537 22.93726412015435 76-77 22.542759201544214 27.08115536416737 27.470677297887192 22.905408136401224 78-79 22.54917360372589 27.101722015170516 27.482555707957022 22.866548673146568 80-81 22.458364642393605 27.261370312179796 27.357305826900745 22.922959218525857 82-83 22.481658390702368 27.254902067907523 27.48255710087571 22.780882440514404 84-85 22.44615459817653 27.195740812399183 27.333592031937354 23.024512557486933 86-87 22.394608554642918 27.242878572593103 27.365714302879923 22.996798569884056 88-89 22.445737618558788 27.206686564033827 27.1143546969229 23.233221120484483 90-91 22.423795972403514 27.076516903925523 27.41394808410706 23.0857390395639 92-93 22.492849548715242 27.06900182076038 27.492116274134908 22.94603235638947 94-95 22.341011751097586 26.970426468122614 27.50541625274499 23.183145528034807 96-97 22.258308262289226 26.945976804016027 27.566142088577955 23.22957284511679 98-99 22.159684587414148 27.021120656568677 27.299835633831403 23.519359122185772 100-101 22.273256447757433 26.837758065349004 27.346464044003515 23.542521442890045 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 190.0 1 158.5 2 242.0 3 371.0 4 322.5 5 288.5 6 494.0 7 990.5 8 1648.0 9 2059.5 10 1954.0 11 1794.0 12 1791.0 13 1867.0 14 2056.5 15 2211.0 16 2289.0 17 2414.0 18 2619.5 19 2875.0 20 3189.5 21 3641.0 22 4312.0 23 5147.0 24 6186.0 25 7650.0 26 9641.0 27 11868.0 28 13996.0 29 16589.0 30 20133.0 31 24070.5 32 28126.0 33 31270.5 34 34691.0 35 38205.5 36 41713.5 37 45241.5 38 47108.5 39 48339.0 40 49940.5 41 53179.0 42 56265.0 43 59553.5 44 62668.5 45 64601.0 46 65634.5 47 68167.5 48 74881.0 49 75120.5 50 69552.0 51 66293.0 52 63652.5 53 62581.0 54 61780.0 55 58404.0 56 53761.5 57 49095.5 58 45250.0 59 40699.5 60 34223.5 61 28138.0 62 23152.5 63 18283.5 64 13921.0 65 10687.0 66 8049.5 67 6408.5 68 4800.0 69 3227.0 70 2361.0 71 1796.5 72 1411.0 73 1131.5 74 904.0 75 723.0 76 518.0 77 296.5 78 171.0 79 111.0 80 67.5 81 44.5 82 30.5 83 18.0 84 12.5 85 10.0 86 9.5 87 6.5 88 4.0 89 7.0 90 6.0 91 2.0 92 1.5 93 2.0 94 2.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012941774573945313 2 0.012395708136226102 3 0.015289860256137924 4 0.0188392921013128 5 0.029924440787012792 6 0.04614261398727337 7 0.005296844445876352 8 0.0237538900407857 9 0.033091626125784215 10-11 0.03980824330973052 12-13 0.030607023834161806 14-15 0.02604736907920639 16-17 0.029869834143240872 18-19 0.02517366277885565 20-21 0.05225855808972855 22-23 0.012996381217717236 24-25 0.002921455441797782 26-27 0.0011194361973243837 28-29 1.365166094298029E-4 30-31 0.0027576355104820182 32-33 0.002156962428990886 34-35 0.0 36-37 3.0033654074556633E-4 38-39 0.0 40-41 0.001665502635043595 42-43 0.004313924857981772 44-45 0.002320782360306649 46-47 0.0012013461629822653 48-49 0.0012832561286401471 50-51 0.003494825201402954 52-53 0.003576735167060836 54-55 0.005569877664735958 56-57 0.001037526231666502 58-59 0.00521493448021847 60-61 0.0026211189010522155 62-63 6.279764033770933E-4 64-65 7.371896909209357E-4 66-67 0.016928059569295557 68-69 0.040654646288195304 70-71 0.04846339634758002 72-73 0.01321480779280492 74-75 0.027330625207846536 76-77 0.024299956478504914 78-79 0.052012828192754906 80-81 0.0766677278557773 82-83 0.047944633231746775 84-85 0.01744682268512881 86-87 0.02066861466767216 88-89 0.020941647886531762 90-91 0.03732364101810811 92-93 0.02460029301925048 94-95 0.01179503505473497 96-97 0.017228396110041126 98-99 0.049937775729421895 100-101 0.06790336153038395 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1831279.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.760567451813856 #Duplication Level Percentage of deduplicated Percentage of total 1 81.34794360865169 50.240951583084836 2 11.21368709613096 13.851273565682614 3 3.1694649558700148 5.872438625795108 4 1.3383839737888168 3.3063741475844357 5 0.7367767064904008 2.2751873739062827 6 0.4589246177204932 1.7006066884814646 7 0.2944030568585443 1.2727749895792622 8 0.21624266042636853 1.068421553217793 9 0.15053687121856912 0.8367518329991509 >10 0.9650134934864524 11.001748564219675 >50 0.07392440894764979 3.137160109757138 >100 0.03239173816209383 3.5279248678890456 >500 0.0015970238638805623 0.5947575934061317 >1k 5.323412879601871E-4 0.6405123926040418 >5k 1.7744709598672905E-4 0.673116111792972 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6585 0.3595847492381008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5699 0.31120326285617866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3069 0.16758778973602603 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2902 0.15846848022611518 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1887 0.10304273679761522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.460664377192115E-5 2 0.0 0.0 0.0 0.0 5.460664377192115E-5 3 0.0 0.0 0.0 0.0 5.460664377192115E-5 4 0.0 0.0 0.0 0.0 5.460664377192115E-5 5 0.0 0.0 0.0 0.0 5.460664377192115E-5 6 0.0 0.0 0.0 0.0 5.460664377192115E-5 7 0.0 0.0 0.0 0.0 5.460664377192115E-5 8 0.0 0.0 0.0 0.0 5.460664377192115E-5 9 0.0 0.0 0.0 0.0 5.460664377192115E-5 10-11 0.0 0.0 0.0 1.6381993131576347E-4 5.460664377192115E-5 12-13 0.0 0.0 0.0 1.9112325320172404E-4 5.460664377192115E-5 14-15 0.0 0.0 0.0 2.457298969736452E-4 5.460664377192115E-5 16-17 2.7303321885960576E-5 0.0 0.0 2.730332188596058E-4 5.460664377192115E-5 18-19 5.460664377192115E-5 0.0 0.0 2.730332188596058E-4 5.460664377192115E-5 20-21 5.460664377192115E-5 0.0 0.0 3.2763986263152693E-4 5.460664377192115E-5 22-23 5.460664377192115E-5 0.0 0.0 3.549431845174875E-4 5.460664377192115E-5 24-25 5.460664377192115E-5 0.0 0.0 4.368531501753692E-4 5.460664377192115E-5 26-27 5.460664377192115E-5 0.0 0.0 5.18763115833251E-4 5.460664377192115E-5 28-29 8.190996565788173E-5 0.0 0.0 0.0012013461629822655 5.460664377192115E-5 30-31 1.092132875438423E-4 0.0 0.0 0.002921455441797782 5.460664377192115E-5 32-33 1.092132875438423E-4 0.0 0.0 0.0056790909522798 5.460664377192115E-5 34-35 1.092132875438423E-4 0.0 0.0 0.008600546394077582 5.460664377192115E-5 36-37 1.092132875438423E-4 0.0 0.0 0.011713125089077087 5.460664377192115E-5 38-39 1.092132875438423E-4 0.0 0.0 0.015262556934251964 5.460664377192115E-5 40-41 1.092132875438423E-4 0.0 0.0 0.0197403017235495 5.460664377192115E-5 42-43 1.092132875438423E-4 0.0 0.0 0.025828942504118706 5.460664377192115E-5 44-45 1.092132875438423E-4 0.0 0.0 0.03202679657223176 5.460664377192115E-5 46-47 1.092132875438423E-4 0.0 0.0 0.039262176872011306 5.460664377192115E-5 48-49 1.092132875438423E-4 0.0 0.0 0.04551463758389628 5.460664377192115E-5 50-51 1.092132875438423E-4 0.0 0.0 0.055098103565868445 5.460664377192115E-5 52-53 1.092132875438423E-4 0.0 0.0 0.08029906966661006 5.460664377192115E-5 54-55 1.092132875438423E-4 0.0 0.0 0.1176773173284901 5.460664377192115E-5 56-57 1.6381993131576347E-4 0.0 0.0 0.14361547312015266 5.460664377192115E-5 58-59 1.6381993131576347E-4 0.0 0.0 0.180775294206945 5.460664377192115E-5 60-61 1.6381993131576347E-4 0.0 0.0 0.22609880853763953 5.460664377192115E-5 62-63 1.6381993131576347E-4 0.0 0.0 0.25239190751381957 5.460664377192115E-5 64-65 1.6381993131576347E-4 0.0 0.0 0.27494445139162305 5.460664377192115E-5 66-67 1.6381993131576347E-4 0.0 0.0 0.3450320786728838 5.460664377192115E-5 68-69 1.6381993131576347E-4 0.0 0.0 0.42159059324111725 5.460664377192115E-5 70-71 1.6381993131576347E-4 0.0 0.0 0.4517061572813318 5.460664377192115E-5 72-73 1.6381993131576347E-4 0.0 0.0 0.5155413238507076 5.460664377192115E-5 74-75 1.6381993131576347E-4 0.0 0.0 0.6059426226151231 5.460664377192115E-5 76-77 1.6381993131576347E-4 5.460664377192115E-5 0.0 0.6948422386758107 5.460664377192115E-5 78-79 1.6381993131576347E-4 5.460664377192115E-5 0.0 0.7869909500409277 5.460664377192115E-5 80-81 1.6381993131576347E-4 5.460664377192115E-5 0.0 0.9060061301418298 5.460664377192115E-5 82-83 1.6381993131576347E-4 5.460664377192115E-5 0.0 1.0454988016572024 5.460664377192115E-5 84-85 1.6381993131576347E-4 5.460664377192115E-5 0.0 1.1775922729414798 5.460664377192115E-5 86-87 1.6381993131576347E-4 5.460664377192115E-5 0.0 1.3950632317631557 5.460664377192115E-5 88-89 1.6381993131576347E-4 5.460664377192115E-5 0.0 1.6729291385965765 5.460664377192115E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1705 0.0 31.485012 2 GTACCTG 1860 0.0 29.37208 1 GGTATCA 7840 0.0 28.297667 1 GTACATG 8265 0.0 26.325245 1 GTATCAA 10780 0.0 25.956514 1 TACATGG 8335 0.0 25.4772 2 TATCACG 95 0.0012259074 25.003235 2 ACATGGG 8420 0.0 24.090939 3 ATCAACG 12585 0.0 22.195353 3 TCAACGC 12680 0.0 22.029665 4 GAGTACT 6495 0.0 21.90682 12-13 CAACGCA 12820 0.0 21.714977 5 TATCAAC 12885 0.0 21.605434 2 TAGCGCG 110 0.0028749385 21.594883 9 AACGCAG 13025 0.0 21.483213 6 GTACTTT 6930 0.0 20.394888 14-15 CATGGGA 5000 0.0 19.857569 4 ACGCAGA 14490 0.0 19.11393 7 CATGGGG 3845 0.0 19.027168 4 CGCAGAG 14575 0.0 19.002459 8 >>END_MODULE