FastQCFastQC Report
Thu 26 May 2016
SRR936571_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936571_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1831279
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69440.3791885343522205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65520.3577827299936274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.21285669742294866No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35650.1946726850468989No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.10637374206770242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81900.027.6131971
TACATGG85900.025.8267572
TACCTGG18500.025.1652092
GGTATCA96050.025.1281191
ACATGGG85200.024.4737173
GTACCTG19750.024.2967321
GTATCAA127300.023.8114531
GAGTACT77250.022.10513512-13
ACCGGTC1302.9443702E-421.920948
CATGGGG38600.021.5334644
GTACTTT82150.020.5556314-15
CGAATAC1404.854651E-420.3562723
AGTACTT79950.020.05155812-13
TCAACGC149750.019.9819054
ATCAACG150300.019.9093273
TATCAAC151000.019.820282
CAACGCA152100.019.6419495
AACGCAG155300.019.2061146
CATGGGA48950.018.9210434
TATAACG1801.232684E-418.4744592
Produced by FastQC (version 0.11.5)