##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936571_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1831279 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.834243717096083 31.0 31.0 34.0 30.0 34.0 2 31.941149873940564 31.0 31.0 34.0 30.0 34.0 3 32.06138387433045 33.0 31.0 34.0 30.0 34.0 4 35.81808178873891 37.0 35.0 37.0 35.0 37.0 5 35.67130404487792 37.0 35.0 37.0 33.0 37.0 6 35.71974723676731 37.0 35.0 37.0 33.0 37.0 7 35.6364355185638 37.0 35.0 37.0 33.0 37.0 8 35.6038937813408 37.0 35.0 37.0 33.0 37.0 9 37.19161198266348 39.0 37.0 39.0 33.0 39.0 10-11 37.089303432191386 39.0 37.0 39.0 33.0 39.0 12-13 36.96137153322896 39.0 37.0 39.0 33.0 39.0 14-15 38.0334132592576 40.0 37.0 41.0 33.0 41.0 16-17 38.00156420731085 40.0 37.0 41.0 33.0 41.0 18-19 38.14329493212121 40.0 37.0 41.0 33.0 41.0 20-21 38.152834985821386 40.0 37.0 41.0 33.0 41.0 22-23 38.05696401258355 40.0 37.0 41.0 33.0 41.0 24-25 37.94293332692615 40.0 37.0 41.0 33.0 41.0 26-27 37.73516296533734 40.0 37.0 41.0 32.5 41.0 28-29 37.74352215036595 40.0 37.0 41.0 32.5 41.0 30-31 37.61964179133818 39.5 37.0 41.0 32.0 41.0 32-33 37.42450795318463 39.0 36.0 41.0 32.0 41.0 34-35 37.2465167787104 39.0 36.0 41.0 31.0 41.0 36-37 37.067893805367724 39.0 36.0 41.0 31.0 41.0 38-39 36.867611379806135 39.0 35.5 41.0 31.0 41.0 40-41 36.64255828849673 39.0 35.0 40.0 30.0 41.0 42-43 36.372852525475366 39.0 35.0 40.0 30.0 41.0 44-45 36.091378211621496 39.0 35.0 40.0 30.0 41.0 46-47 35.70723248614766 38.0 35.0 40.0 28.5 41.0 48-49 35.31616127307745 38.0 34.5 40.0 27.0 41.0 50-51 34.9988491649825 38.0 34.0 40.0 26.0 41.0 52-53 34.72330895510733 38.0 34.0 40.0 26.0 41.0 54-55 34.38280021777129 38.0 33.0 40.0 25.0 41.0 56-57 34.028744118181876 37.5 33.0 40.0 24.0 41.0 58-59 33.64850877446855 37.0 33.0 40.0 23.0 41.0 60-61 33.266645333671164 36.5 32.0 39.0 22.5 40.0 62-63 32.92742094459665 36.0 32.0 39.0 22.0 40.0 64-65 32.58748284668802 35.5 31.0 39.0 21.5 40.0 66-67 32.12576073880605 35.0 31.0 38.0 19.5 40.0 68-69 31.66862040136975 35.0 30.5 38.0 18.5 40.0 70-71 31.15610210131826 34.5 30.0 37.0 17.0 39.0 72-73 30.587701819329553 34.0 29.0 36.5 12.5 39.0 74-75 30.036421812296215 34.0 29.0 36.0 8.0 39.0 76-77 28.671308959475866 32.0 27.0 34.5 8.0 36.5 78-79 29.443011960493187 33.5 29.0 35.0 7.0 37.0 80-81 29.287746159924296 34.0 29.0 35.0 6.0 37.0 82-83 28.879134746808106 33.5 29.0 35.0 2.0 36.0 84-85 28.411797164713843 33.0 27.5 35.0 2.0 36.0 86-87 27.965898697030873 33.0 27.0 35.0 2.0 35.5 88-89 27.44830716673975 32.5 26.0 35.0 2.0 35.0 90-91 26.8440608995134 32.0 25.0 34.0 2.0 35.0 92-93 26.23292218171016 32.0 24.5 34.0 2.0 35.0 94-95 25.459296207732407 31.5 22.0 34.0 2.0 35.0 96-97 24.580297704500516 31.0 18.5 34.0 2.0 35.0 98-99 23.395903354977587 31.0 2.0 34.0 2.0 35.0 100-101 19.639612806131673 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3514624362081875 1101 2 0.4856383651227425 1101 3 0.41230978694979825 1101 4 0.26913872728550103 1101 5 0.18114765633306007 1101 6 0.2529428653465118 1101 7 0.20888356478504022 1101 8 0.171373529845944 1101 9 0.3299167519940269 1101 10-11 0.38779894397156056 1101 12-13 0.34978564907567744 1101 14-15 0.4862387392074794 1101 16-17 0.5155781049543364 1101 18-19 0.42306251834318687 1101 20-21 0.272821186577211 1101 22-23 0.5228402410113873 1101 24-25 0.42601431088959885 1101 26-27 0.3047218565271024 1101 28-29 0.48047755896281075 1101 30-31 0.49776645822962706 1101 32-33 0.4520025164544563 1101 34-35 0.630298404651839 1101 36-37 0.5350302691522444 1101 38-39 0.6676135087857986 1101 40-41 0.7180477618910501 1101 42-43 0.6632087889504348 1101 44-45 0.6828004624244954 1101 46-47 0.7618905113610239 1101 48-49 0.5892735575808956 1101 50-51 0.623309456838804 1101 52-53 0.7192860093743718 1101 54-55 0.7646919424197378 1101 56-57 0.7373963898713995 1101 58-59 0.7651848389698941 1101 60-61 0.7423807256025157 1101 62-63 0.7316591635351699 1101 64-65 0.736116876121848 1101 66-67 0.8183365639211075 1101 68-69 0.7036716411828685 1101 70-71 0.4803448303489617 1101 72-73 0.690510456229795 1101 74-75 0.6777509419871244 1101 76-77 0.6982432995184951 1101 78-79 0.7042705427356175 1101 80-81 0.8161192031419446 1101 82-83 0.7930415314716939 1101 84-85 0.6258787927975504 1101 86-87 0.9117510216313356 1101 88-89 0.7344364119298312 1101 90-91 0.7309617232513403 1101 92-93 0.8494910334454318 1101 94-95 0.8052054332664333 1101 96-97 0.9208348809812463 1101 98-99 0.08488889708306502 1101 100-101 0.41607003789915 1102 1 0.24897057828221847 1102 2 0.4068108532658776 1102 3 0.2981625279345508 1102 4 0.20061116019815017 1102 5 0.10988141395181827 1102 6 0.21668483874546496 1102 7 0.3098813988066311 1102 8 0.2642103719201714 1102 9 0.2623829896580716 1102 10-11 0.4473841109504093 1102 12-13 0.4089656778505599 1102 14-15 0.46220831920028616 1102 16-17 0.5557767086648084 1102 18-19 0.45947852181819115 1102 20-21 0.47647082089636683 1102 22-23 0.4252321533816428 1102 24-25 0.39416772092975094 1102 26-27 0.5276387729757701 1102 28-29 0.5946549146608859 1102 30-31 0.6920454596388979 1102 32-33 0.5503826761314841 1102 34-35 0.6154424363199382 1102 36-37 0.6497626322504146 1102 38-39 0.6664385097194057 1102 40-41 0.6722655478758739 1102 42-43 0.7240777944282755 1102 44-45 0.7796766478652728 1102 46-47 0.7468507689984634 1102 48-49 0.7930554944609014 1102 50-51 0.7006312746489627 1102 52-53 0.697722411766776 1102 54-55 0.8540849869166394 1102 56-57 0.9667053503786533 1102 58-59 0.8779596892481081 1102 60-61 0.9153661396444264 1102 62-63 0.8154308847411187 1102 64-65 0.6971037131840561 1102 66-67 0.732008590172299 1102 68-69 0.6757974448257222 1102 70-71 0.41462706500186286 1102 72-73 0.2287710792532387 1102 74-75 0.46800138569821925 1102 76-77 0.4786819789399317 1102 78-79 0.5860176048079957 1102 80-81 0.5731534735590422 1102 82-83 0.5785500892484805 1102 84-85 0.42123486235258056 1102 86-87 0.5534555405069916 1102 88-89 0.5720178818076 1102 90-91 0.6778319876304266 1102 92-93 1.0290259770454746 1102 94-95 1.1356566918728568 1102 96-97 0.9393351276512014 1102 98-99 1.0470714937806314 1102 100-101 1.165448326422414 1103 1 0.2266269930910454 1103 2 0.26782049582679335 1103 3 0.23234583371678497 1103 4 0.1380981697416459 1103 5 0.26009406893889064 1103 6 0.14324918108356854 1103 7 0.15917000741461607 1103 8 0.15810675129783647 1103 9 0.07839854712590721 1103 10-11 0.30080599144403664 1103 12-13 0.30968957407453956 1103 14-15 0.34183097921842887 1103 16-17 0.2349126757666511 1103 18-19 0.2710452728540247 1103 20-21 0.2431364839389829 1103 22-23 0.1533780777291014 1103 24-25 0.19574796747161827 1103 26-27 0.2434600320642275 1103 28-29 0.3362748561528477 1103 30-31 0.41583739574624445 1103 32-33 0.46674149730704784 1103 34-35 0.4557863264833202 1103 36-37 0.4127745776429066 1103 38-39 0.33183700836257657 1103 40-41 0.3783761372103882 1103 42-43 0.4838893526808832 1103 44-45 0.4532934920438976 1103 46-47 0.4564373374006223 1103 48-49 0.418094951454961 1103 50-51 0.49179997381621376 1103 52-53 0.702354420057361 1103 54-55 0.6064628474880109 1103 56-57 0.6198322382440651 1103 58-59 0.6828284637842827 1103 60-61 0.4058051636556428 1103 62-63 0.4937616863518386 1103 64-65 0.656325820367222 1103 66-67 0.6934377394940725 1103 68-69 0.60969471775255 1103 70-71 0.3415953255530262 1103 72-73 0.5015306018648893 1103 74-75 0.5777034841789899 1103 76-77 0.6123185686124444 1103 78-79 0.5669779661903576 1103 80-81 0.8558945230131023 1103 82-83 0.6321898694742352 1103 84-85 0.7079876561505749 1103 86-87 0.5254689981343112 1103 88-89 0.5417903035783418 1103 90-91 0.7302156999775313 1103 92-93 0.7367950955980795 1103 94-95 0.7197629776026844 1103 96-97 0.9459172826975184 1103 98-99 0.8326626736734468 1103 100-101 0.8150528580763918 1104 1 0.1935987782169306 1104 2 0.24151497852201587 1104 3 0.21954707857699418 1104 4 0.15713157410029055 1104 5 0.08185678984214206 1104 6 0.14383998912125406 1104 7 0.10416084567857098 1104 8 0.16483787179235065 1104 9 0.2289761432038091 1104 10-11 0.21107392228749688 1104 12-13 0.16137533832023365 1104 14-15 0.2505460836410407 1104 16-17 0.21251058264567746 1104 18-19 0.20318729973710958 1104 20-21 0.25203686865007313 1104 22-23 0.15169156745290024 1104 24-25 0.10732963398827877 1104 26-27 0.18305658838736605 1104 28-29 0.20959815256809122 1104 30-31 0.349239732891391 1104 32-33 0.43094851740606543 1104 34-35 0.26793463927514694 1104 36-37 0.29839293827213 1104 38-39 0.49116109027576016 1104 40-41 0.30310177847174913 1104 42-43 0.3342412865604345 1104 44-45 0.2971826357596967 1104 46-47 0.17424828652951163 1104 48-49 0.3500724906420203 1104 50-51 0.26427330081871503 1104 52-53 0.3633656138842696 1104 54-55 0.29485251896023357 1104 56-57 0.4936818471556137 1104 58-59 0.4092579906132343 1104 60-61 0.31295937971474785 1104 62-63 0.3183535474958248 1104 64-65 0.2834011689680267 1104 66-67 0.45081574064248287 1104 68-69 0.505667500634079 1104 70-71 0.39648861190408624 1104 72-73 0.4986319123276566 1104 74-75 0.5608579947998145 1104 76-77 0.4957863157879956 1104 78-79 0.6137511916845781 1104 80-81 0.5399885026431015 1104 82-83 0.46536192384494157 1104 84-85 0.46102388002325867 1104 86-87 0.5456820933282849 1104 88-89 0.48574476106254494 1104 90-91 0.48493157545878063 1104 92-93 0.4210737779445459 1104 94-95 0.6888378468187213 1104 96-97 0.8586342392010522 1104 98-99 0.7479938654981062 1104 100-101 0.532055687697941 1105 1 0.14429193887081482 1105 2 0.23738297317366985 1105 3 0.2381240995213929 1105 4 0.2170988788194208 1105 5 0.1996603411785074 1105 6 0.14859026745499193 1105 7 0.19139105663064981 1105 8 0.22040506394588277 1105 9 0.18007343606018367 1105 10-11 0.1546100002041797 1105 12-13 0.18864588363749846 1105 14-15 0.26341313548184075 1105 16-17 0.2188456013417266 1105 18-19 0.21526789552654435 1105 20-21 0.18314735987276265 1105 22-23 0.034206639859093 1105 24-25 0.19449971795413745 1105 26-27 0.23465096385279338 1105 28-29 0.18904458016263703 1105 30-31 0.18799936245002158 1105 32-33 0.16431921653999382 1105 34-35 -0.03873590771778623 1105 36-37 0.17719024083753254 1105 38-39 0.20414750896953393 1105 40-41 0.24384232116215543 1105 42-43 0.3957520559443637 1105 44-45 0.3436685584058381 1105 46-47 0.3849481547922764 1105 48-49 0.24832863554608764 1105 50-51 0.3081451780965807 1105 52-53 0.4141071225492112 1105 54-55 0.3303573811141902 1105 56-57 0.40420820963559123 1105 58-59 0.4992901437655348 1105 60-61 0.48692215223907453 1105 62-63 0.5439046581447613 1105 64-65 0.3415344773963014 1105 66-67 0.31667280401090636 1105 68-69 0.37054466662251784 1105 70-71 0.2572171390541307 1105 72-73 0.3528158388071354 1105 74-75 0.47016099489000496 1105 76-77 0.29696103446023514 1105 78-79 0.406510719717307 1105 80-81 0.4164291476972366 1105 82-83 0.46673437158990794 1105 84-85 0.3920269312213911 1105 86-87 0.4403403527308889 1105 88-89 0.42121482841955427 1105 90-91 0.5310266726944661 1105 92-93 0.40023460235452646 1105 94-95 0.48181176205642373 1105 96-97 0.45830667033717276 1105 98-99 0.5393651747730921 1105 100-101 0.20772567235570705 1106 1 0.07969687861650243 1106 2 0.17085488217331957 1106 3 0.1356153818188588 1106 4 0.09465722095767859 1106 5 0.09687781583787114 1106 6 -0.006013807979783792 1106 7 0.06957787686160799 1106 8 0.09021797656055952 1106 9 0.1677275264264253 1106 10-11 0.08364110756671295 1106 12-13 0.09572823806587394 1106 14-15 0.12103508287852804 1106 16-17 0.26359203883566806 1106 18-19 0.11296171870771587 1106 20-21 -0.06100051365267234 1106 22-23 0.04975558706873073 1106 24-25 0.1921384469441989 1106 26-27 0.038432093798057565 1106 28-29 0.22372334110885816 1106 30-31 0.20122567879302977 1106 32-33 0.20684077505307386 1106 34-35 0.19702155296458557 1106 36-37 0.3430929756640495 1106 38-39 0.28707143164523785 1106 40-41 0.11183415708843114 1106 42-43 0.36408006111754077 1106 44-45 0.4350349685548309 1106 46-47 0.25891808150139184 1106 48-49 0.3736285124861851 1106 50-51 0.31400594102701973 1106 52-53 0.23686734408261856 1106 54-55 0.2675135382163134 1106 56-57 0.3177703369725151 1106 58-59 0.15155469926442322 1106 60-61 0.1208629632163607 1106 62-63 0.1503441787715616 1106 64-65 0.2994927219873844 1106 66-67 -0.015280189103222597 1106 68-69 0.061495554488562476 1106 70-71 0.18595985276419214 1106 72-73 0.1709355326646076 1106 74-75 0.10460463952044918 1106 76-77 0.10033631495313244 1106 78-79 0.19463056455282057 1106 80-81 0.4058164148232031 1106 82-83 0.3561910630263547 1106 84-85 0.38410531799796743 1106 86-87 0.4108133976483934 1106 88-89 0.2854125093456261 1106 90-91 0.4420134728632519 1106 92-93 0.341815311761966 1106 94-95 0.2912046691079446 1106 96-97 0.3332356914646333 1106 98-99 0.5140281162911045 1106 100-101 0.3158683020810287 1107 1 0.05264039433538059 1107 2 0.13060817179612982 1107 3 0.08847474420993251 1107 4 0.0905322251578724 1107 5 0.05672204311052553 1107 6 0.050312865659662975 1107 7 -0.008423425600220469 1107 8 -0.04315848770894348 1107 9 0.12331128480215625 1107 10-11 0.07447227235267206 1107 12-13 0.16376826769928243 1107 14-15 0.20325298548830517 1107 16-17 0.3498371992726135 1107 18-19 0.3116914184107955 1107 20-21 0.25593879706661227 1107 22-23 0.07515172587211794 1107 24-25 0.1238336047410229 1107 26-27 0.14038653323544992 1107 28-29 0.2311663610708692 1107 30-31 0.16057068236920458 1107 32-33 0.2255107279201667 1107 34-35 0.03344938873918579 1107 36-37 0.07256440124105268 1107 38-39 0.2408585714081326 1107 40-41 0.3699941444158341 1107 42-43 0.3912229494999764 1107 44-45 0.2161093768867488 1107 46-47 0.31046986182590075 1107 48-49 0.33446452016868733 1107 50-51 0.365108900047467 1107 52-53 0.36213422341715784 1107 54-55 0.34432745098337136 1107 56-57 0.4783460923016989 1107 58-59 0.2788068728342594 1107 60-61 0.19201500723337972 1107 62-63 -0.17781206437810937 1107 64-65 -0.008790711199573309 1107 66-67 0.09736635785287717 1107 68-69 0.06661606420875898 1107 70-71 0.06373018426259591 1107 72-73 0.1320699545118309 1107 74-75 0.1787204242163618 1107 76-77 -0.026092886219618805 1107 78-79 0.12066932052577073 1107 80-81 0.2009870922580248 1107 82-83 0.2255165936782113 1107 84-85 0.14092270826342101 1107 86-87 0.39960579899437576 1107 88-89 0.40474084013039757 1107 90-91 0.4375208596138762 1107 92-93 0.33637825368294116 1107 94-95 0.3879959301331368 1107 96-97 0.5873580204711821 1107 98-99 0.7492072794620519 1107 100-101 0.389341256263922 1108 1 0.042262037577032174 1108 2 0.16409691493262457 1108 3 -0.03407557351393109 1108 4 0.047795811245492814 1108 5 0.0028380983020639405 1108 6 0.10107231523864613 1108 7 0.1828146920328777 1108 8 0.06784377915711559 1108 9 0.07899037216605365 1108 10-11 0.08976784949149419 1108 12-13 0.02578062503121714 1108 14-15 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121.0 7 305.0 8 775.0 9 1466.0 10 2471.0 11 3835.0 12 5436.0 13 7611.0 14 9686.0 15 12549.0 16 15761.0 17 19559.0 18 17930.0 19 13355.0 20 12865.0 21 13523.0 22 14723.0 23 16424.0 24 19259.0 25 23202.0 26 27713.0 27 34333.0 28 41575.0 29 51369.0 30 62066.0 31 76580.0 32 94095.0 33 118302.0 34 152356.0 35 201974.0 36 273487.0 37 324405.0 38 156353.0 39 5716.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.494310098407965 19.285153717728235 14.426851027087723 22.793685156776082 2 16.097646223581457 20.61285566053192 38.93528480148545 24.35421331440118 3 19.92198464594922 24.39052893820262 29.762371544437517 25.925114871410642 4 13.355955119744573 16.670043188536063 36.17155934696793 33.80244234475144 5 13.661963139594762 37.53865678887755 33.870419003276034 14.928961068251656 6 32.42405990567248 35.86864699480527 16.853521500546886 14.85377159897536 7 29.037942458606665 32.727397826539246 20.04332505311879 18.191334661735308 8 27.143311947130993 34.617797856428666 19.02565634380582 19.213233852634524 9 26.622173149024892 16.492962837974353 20.132279206106336 36.752584806894426 10-11 25.52087912327941 26.578964756326044 26.526979231455172 21.37317688893937 12-13 26.40837360118256 23.829847882272446 27.408849225049813 22.35292929149518 14-15 22.976872931085136 25.312951529918514 26.012246119368264 25.697929419628085 16-17 22.255785556777585 28.713388721481785 26.65479101659132 22.376034705149305 18-19 22.158052121945516 26.914381698998056 29.23294219667068 21.694623982385743 20-21 22.924403787755924 25.86867358099766 29.452254147260724 21.754668483985697 22-23 22.548045786703682 25.925967386639027 29.45263789034301 22.073348936314282 24-25 22.516866032006757 26.09069828334934 29.562415581103707 21.830020103540193 26-27 22.3343267200083 26.232097538467 29.540771401385097 21.8928043401396 28-29 22.01962837418141 26.50876232575347 29.556388914296168 21.91522038576895 30-31 22.241962615572085 26.107202622441633 29.51663885810425 22.13419590388203 32-33 22.088500900769052 26.318729716313722 29.462492894054375 22.130276488862847 34-35 22.68839740026277 26.254517376541425 29.19513974515935 21.861945478036457 36-37 22.610718028276843 26.068217846706975 29.197171769462514 22.12389235555366 38-39 22.40962111862296 26.26301073624657 29.358036894243057 21.969331250887407 40-41 22.495983679157398 26.212213547042012 29.26704738439494 22.02475538940565 42-43 22.377487767138636 26.36089132173333 28.89180555968907 22.36981535143896 44-45 22.519396725322164 26.429842082053984 28.69778789272993 22.35297329989392 46-47 22.858149959672993 26.777214176540003 28.163567648621534 22.201068215165467 48-49 22.94324033573063 26.819815096956688 27.93493990378052 22.302004663532163 50-51 22.714045364418688 27.00191507591225 27.921555607371758 22.3624839522973 52-53 23.024493997318793 27.099382121102078 27.599773926766467 22.27634995481266 54-55 22.718745401095465 26.954420095031967 27.691637290559573 22.635197213312992 56-57 22.816414814216092 27.221855253129938 27.526732386143475 22.434997546510488 58-59 23.145514832604864 26.865652488264757 27.540142286412017 22.44869039271836 60-61 22.844794279634 26.929139534128087 28.055006207639043 22.171059978598866 62-63 22.741038610846118 27.00154482701378 27.99517057260168 22.262245989538425 64-65 22.61870046297358 27.13160070467222 27.791439084406672 22.45825974794753 66-67 22.6948445282003 27.22937383976014 27.48196107737426 22.5938205546653 68-69 22.610450462651116 27.06967057012477 27.73613172935599 22.58374723786812 70-71 22.76555647360204 26.99514466877423 27.653353051005823 22.585945806617907 72-73 22.840032372073516 26.89628922547302 27.658108745702293 22.60556965675117 74-75 22.648146226646933 27.390900700660247 27.347566803012345 22.613386269680472 76-77 22.795375625323206 27.2416482048084 27.37448075170389 22.588495418164502 78-79 22.809475505046713 27.19771945656878 27.331351874581035 22.661453163803472 80-81 22.74573406563102 27.367400739123728 27.263153439846878 22.623711755398375 82-83 22.733020297797875 27.39810895373192 27.32646227887212 22.542408469598083 84-85 22.714256147913616 27.302726963446993 27.21677385321677 22.76624303542262 86-87 22.686200725947064 27.362863772161617 27.107930428343717 22.8430050735476 88-89 22.74344179666211 27.351740753968123 26.989850471107207 22.914966978262555 90-91 22.73440070557428 27.2678246931885 27.151885513473218 22.845889087763993 92-93 22.76118710190387 27.175112056688018 27.281216415758706 22.782484425649404 94-95 22.591194858652816 27.13418713014076 27.347645433144518 22.926972578061903 96-97 22.61032388028364 26.99785969201298 27.345649501154522 23.046166926548857 98-99 22.493294929975942 27.120755696818073 27.01042203920954 23.375527333996445 100-101 22.339110583598114 27.30775332159808 27.036406314974954 23.316729779828854 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 355.0 1 278.5 2 306.5 3 424.0 4 351.5 5 314.0 6 547.0 7 1068.0 8 1640.0 9 2007.5 10 1958.0 11 1753.5 12 1806.5 13 1947.0 14 2105.0 15 2349.0 16 2535.0 17 2660.5 18 2899.0 19 3154.5 20 3440.5 21 3937.5 22 4577.5 23 5332.5 24 6390.5 25 7873.0 26 9772.0 27 12133.5 28 14395.5 29 17022.0 30 20510.0 31 24256.0 32 28299.0 33 31577.0 34 35231.5 35 38546.5 36 41644.0 37 45002.5 38 47112.5 39 48799.0 40 50282.0 41 53279.5 42 56140.5 43 59178.5 44 62291.0 45 63958.5 46 65317.0 47 68239.0 48 75351.0 49 75395.5 50 69271.0 51 65822.0 52 63176.0 53 62406.5 54 61252.0 55 57861.5 56 53299.0 57 48660.0 58 45010.0 59 40534.0 60 34044.5 61 27742.0 62 22817.0 63 18034.0 64 13611.0 65 10519.5 66 8029.5 67 6390.0 68 4727.5 69 3107.5 70 2200.5 71 1719.0 72 1402.0 73 1153.0 74 928.0 75 725.5 76 498.5 77 286.0 78 165.0 79 112.0 80 74.5 81 45.5 82 31.5 83 22.0 84 17.0 85 9.0 86 2.5 87 2.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012450314779998025 2 0.009774589235173888 3 0.0055152710209640365 4 3.2763986263152693E-4 5 0.005460664377192116 6 0.0 7 3.2763986263152693E-4 8 0.0019658391757891615 9 5.460664377192115E-5 10-11 0.0 12-13 0.0 14-15 2.730332188596058E-4 16-17 0.004423138145525613 18-19 0.0019112325320172405 20-21 5.460664377192116E-4 22-23 2.730332188596058E-4 24-25 5.18763115833251E-4 26-27 8.190996565788173E-5 28-29 8.190996565788173E-5 30-31 4.641564720613298E-4 32-33 0.003604038488946796 34-35 9.283129441226596E-4 36-37 0.0015289860256137923 38-39 0.005406057733420194 40-41 5.460664377192115E-5 42-43 0.0023753890040785703 44-45 0.005815607561709603 46-47 0.0 48-49 0.0026757255448241367 50-51 0.0010921328754384231 52-53 7.644930128068962E-4 54-55 2.7303321885960576E-5 56-57 0.011658518445305166 58-59 0.0023480856821926094 60-61 0.003685948454604678 62-63 3.2763986263152693E-4 64-65 0.003986284995350245 66-67 0.001665502635043595 68-69 0.002156962428990886 70-71 0.005242237802104431 72-73 0.0038770717078064018 74-75 0.008136389922016252 76-77 0.0014197727380699501 78-79 0.007453806874867237 80-81 0.003085275373113545 82-83 0.0038497683859204414 84-85 0.0026211189010522155 86-87 8.737063003507384E-4 88-89 0.00333100527008719 90-91 0.00799987331258645 92-93 0.0034402185576310326 94-95 4.368531501753692E-4 96-97 2.7303321885960576E-5 98-99 8.46402978464778E-4 100-101 0.0024299956478504915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1831279.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.169868112441485 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87872931240506 47.2641387840442 2 11.951924652977821 14.143876108130712 3 3.4290312927756057 6.086859880409019 4 1.5035583714766165 3.558614021585148 5 0.8093750750500377 2.394530822210408 6 0.48777874508044966 1.731708240867745 7 0.33984426264583983 1.4075978139666985 8 0.2418082815190071 1.1446211300780615 9 0.18283907386993817 0.9736707498016669 >10 1.0512405056619913 11.61989050819274 >50 0.08241664668657386 3.318951450405777 >100 0.0384076942353838 4.011592322638224 >500 0.002307640617939682 0.8923344478373327 >1k 5.538337483055238E-4 0.714501116820173 >5k 1.8461124943517458E-4 0.7371126030121583 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6944 0.3791885343522205 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6552 0.3577827299936274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3898 0.21285669742294866 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3565 0.1946726850468989 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1948 0.10637374206770242 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.460664377192115E-5 0.0 0.0 0.0 0.0 7 5.460664377192115E-5 0.0 0.0 0.0 0.0 8 5.460664377192115E-5 0.0 0.0 0.0 0.0 9 5.460664377192115E-5 0.0 0.0 0.0 0.0 10-11 5.460664377192115E-5 0.0 0.0 1.092132875438423E-4 0.0 12-13 5.460664377192115E-5 0.0 0.0 1.365166094298029E-4 0.0 14-15 5.460664377192115E-5 0.0 0.0 1.6381993131576347E-4 0.0 16-17 1.092132875438423E-4 0.0 0.0 2.730332188596058E-4 0.0 18-19 1.092132875438423E-4 0.0 0.0 2.730332188596058E-4 0.0 20-21 1.092132875438423E-4 0.0 0.0 3.822465064034481E-4 0.0 22-23 1.6381993131576347E-4 0.0 0.0 4.368531501753692E-4 0.0 24-25 1.6381993131576347E-4 0.0 0.0 6.006730814911327E-4 0.0 26-27 1.6381993131576347E-4 0.0 0.0 6.825830471490145E-4 0.0 28-29 1.6381993131576347E-4 0.0 0.0 0.001365166094298029 0.0 30-31 1.6381993131576347E-4 0.0 0.0 0.0031944886606573874 0.0 32-33 1.6381993131576347E-4 0.0 0.0 0.005979427493025366 0.0 34-35 1.6381993131576347E-4 0.0 0.0 0.00892818625670911 0.0 36-37 1.6381993131576347E-4 0.0 0.0 0.012013461629822654 0.0 38-39 2.184265750876846E-4 0.0 0.0 0.01561750011876945 0.0 40-41 2.184265750876846E-4 0.0 0.0 0.020122548229952943 0.0 42-43 2.184265750876846E-4 0.0 0.0 0.026320402298065995 0.0 44-45 2.184265750876846E-4 0.0 0.0 0.03260016633183693 0.0 46-47 3.003365407455664E-4 0.0 0.0 0.03997206324104628 0.0 48-49 3.2763986263152693E-4 0.0 0.0 0.04630643391858914 0.0 50-51 3.2763986263152693E-4 0.0 0.0 0.055726079969245536 0.0 52-53 3.2763986263152693E-4 0.0 0.0 0.08092704606998716 0.0 54-55 3.2763986263152693E-4 0.0 0.0 0.11825068708809526 0.0 56-57 3.2763986263152693E-4 0.0 0.0 0.14470760599559107 0.0 58-59 3.2763986263152693E-4 0.0 0.0 0.18184012376049746 0.0 60-61 3.2763986263152693E-4 0.0 0.0 0.22686330155044643 0.0 62-63 3.2763986263152693E-4 0.0 0.0 0.2531837038485124 0.0 64-65 3.2763986263152693E-4 0.0 0.0 0.2754086078636844 0.0 66-67 3.2763986263152693E-4 0.0 0.0 0.3451958986041996 0.0 68-69 3.2763986263152693E-4 0.0 0.0 0.4212902567003717 0.0 70-71 3.549431845174875E-4 0.0 0.0 0.4515969439937879 0.0 72-73 3.822465064034481E-4 0.0 0.0 0.515295593953734 0.0 74-75 3.822465064034481E-4 0.0 0.0 0.6061883525120968 0.0 76-77 3.822465064034481E-4 0.0 0.0 0.695333698469758 0.0 78-79 3.822465064034481E-4 0.0 0.0 0.7874278031911031 0.0 80-81 4.095498282894087E-4 0.0 0.0 0.9060061301418298 0.0 82-83 4.368531501753692E-4 0.0 0.0 1.0441336355629045 0.0 84-85 4.368531501753692E-4 0.0 0.0 1.1742885709932784 0.0 86-87 4.368531501753692E-4 0.0 0.0 1.3896844773516215 0.0 88-89 4.368531501753692E-4 0.0 0.0 1.6642466822368411 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8190 0.0 27.613197 1 TACATGG 8590 0.0 25.826757 2 TACCTGG 1850 0.0 25.165209 2 GGTATCA 9605 0.0 25.128119 1 ACATGGG 8520 0.0 24.473717 3 GTACCTG 1975 0.0 24.296732 1 GTATCAA 12730 0.0 23.811453 1 GAGTACT 7725 0.0 22.105135 12-13 ACCGGTC 130 2.9443702E-4 21.92094 8 CATGGGG 3860 0.0 21.533464 4 GTACTTT 8215 0.0 20.55563 14-15 CGAATAC 140 4.854651E-4 20.356272 3 AGTACTT 7995 0.0 20.051558 12-13 TCAACGC 14975 0.0 19.981905 4 ATCAACG 15030 0.0 19.909327 3 TATCAAC 15100 0.0 19.82028 2 CAACGCA 15210 0.0 19.641949 5 AACGCAG 15530 0.0 19.206114 6 CATGGGA 4895 0.0 18.921043 4 TATAACG 180 1.232684E-4 18.474459 2 >>END_MODULE