FastQCFastQC Report
Thu 26 May 2016
SRR936570_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936570_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883327
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67100.356284383965185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.30961166064098267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.17580590094019785No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27960.14846067623944223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18200.029.5017452
GTACCTG19350.028.4858511
GTATCAA115550.026.8942831
GGTATCA84150.026.5396941
GTACATG85500.024.6756741
TACATGG86900.023.5119882
ATCAACG133750.022.8787733
TCAACGC134050.022.8630164
ACATGGG86550.022.8384673
GAGTACT68700.022.61532412-13
TATCAAC136450.022.6001762
CAACGCA134850.022.5882385
AACGCAG137850.022.199486
GTGTAGC8950.021.7677231
GTACTTT73250.021.11297414-15
ATAACGC2151.0760432E-619.8904553
CATGGGA49600.019.8303034
ACGCAGA153800.019.740687
CGCAGAG154550.019.5833958
GTATAGG5100.019.5659661
Produced by FastQC (version 0.11.5)