##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936570_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1883327 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.06339154060872 31.0 31.0 34.0 28.0 34.0 2 31.099689007803743 31.0 31.0 34.0 27.0 34.0 3 31.132362038031633 31.0 31.0 34.0 27.0 34.0 4 34.77907288537784 37.0 35.0 37.0 32.0 37.0 5 34.69151400686126 37.0 35.0 37.0 32.0 37.0 6 34.80606235666987 37.0 35.0 37.0 32.0 37.0 7 34.631487256328825 37.0 35.0 37.0 30.0 37.0 8 34.61035497287513 37.0 35.0 37.0 30.0 37.0 9 36.07178785202994 39.0 35.0 39.0 30.0 39.0 10-11 35.927823739584255 39.0 35.0 39.0 30.0 39.0 12-13 35.80713094433415 38.0 35.0 39.0 30.0 39.0 14-15 36.830695094372885 39.0 36.0 41.0 30.0 41.0 16-17 36.74961039691992 39.0 36.0 41.0 30.0 41.0 18-19 36.70474829915357 39.0 36.0 41.0 30.0 41.0 20-21 36.60704938653776 39.0 36.0 41.0 30.0 41.0 22-23 36.49039041016244 39.0 36.0 40.5 30.0 41.0 24-25 36.34151424580012 39.0 35.5 40.5 29.0 41.0 26-27 36.13038840307605 39.0 35.0 40.0 28.0 41.0 28-29 36.236684070265014 39.0 35.0 40.0 29.0 41.0 30-31 36.1621431116317 39.0 35.0 40.0 29.0 41.0 32-33 36.003168063751005 38.5 35.0 40.0 29.0 41.0 34-35 35.825518616788266 38.0 35.0 40.0 27.5 41.0 36-37 35.58517134836383 38.0 35.0 40.0 27.0 41.0 38-39 35.39266468329717 38.0 34.0 40.0 26.0 41.0 40-41 35.234451584881434 38.0 34.0 40.0 26.0 41.0 42-43 34.96930166667817 38.0 34.0 40.0 25.0 41.0 44-45 34.693616668799415 38.0 33.5 40.0 24.5 41.0 46-47 34.343865935124384 38.0 33.0 40.0 23.5 41.0 48-49 34.081530185676726 38.0 33.0 40.0 23.0 41.0 50-51 33.37398630190084 37.5 32.0 39.5 21.5 40.5 52-53 33.5642803931553 37.5 33.0 39.5 22.0 40.5 54-55 33.91550166274895 38.0 33.0 40.0 22.0 41.0 56-57 33.82634587620737 38.0 33.0 40.0 22.0 41.0 58-59 33.57950079832127 37.5 33.0 40.0 20.5 41.0 60-61 33.25663573027945 37.0 32.5 40.0 19.5 41.0 62-63 32.92337522904944 36.5 32.0 40.0 18.0 41.0 64-65 32.60343132127347 36.0 31.5 39.0 18.0 41.0 66-67 32.23436264652925 35.5 31.0 39.0 15.0 41.0 68-69 31.8470570962982 35.0 31.0 39.0 13.0 40.0 70-71 31.44551025923804 35.0 31.0 38.0 9.5 40.0 72-73 31.033512767565057 35.0 30.0 37.0 8.0 39.5 74-75 30.59933458183311 35.0 30.0 37.0 7.0 39.0 76-77 30.175256076082377 34.0 29.5 36.0 4.0 39.0 78-79 29.72182127692111 34.0 29.0 36.0 2.0 38.5 80-81 29.304978636211345 34.0 29.0 35.0 2.0 37.0 82-83 28.890482109585854 34.0 29.0 35.0 2.0 37.0 84-85 28.503019921659913 34.0 28.0 35.0 2.0 36.0 86-87 28.143609155499814 34.0 27.0 35.0 2.0 36.0 88-89 27.75739290096728 34.0 26.5 35.0 2.0 36.0 90-91 27.392590346763996 33.0 26.0 35.0 2.0 35.0 92-93 27.005095238373368 33.0 25.5 35.0 2.0 35.0 94-95 26.55659479208868 33.0 25.0 35.0 2.0 35.0 96-97 25.951713377443216 33.0 23.5 35.0 2.0 35.0 98-99 25.260393442031045 32.0 19.0 35.0 2.0 35.0 100-101 23.165076484328 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.46510207390548786 1101 2 0.5669229634524875 1101 3 0.5861883195576318 1101 4 0.5091290750191888 1101 5 0.7048342389244908 1101 6 0.5346338625988523 1101 7 0.5121205842247392 1101 8 0.5691676024789274 1101 9 0.7136635903987312 1101 10-11 0.5310342674921316 1101 12-13 0.8193697635517623 1101 14-15 0.8666640302939754 1101 16-17 1.0418453469070172 1101 18-19 1.0448598485461957 1101 20-21 0.8410619553751957 1101 22-23 0.800133680006553 1101 24-25 0.8554376425442314 1101 26-27 0.8486748038155341 1101 28-29 0.8718468887930797 1101 30-31 0.8457961818265147 1101 32-33 0.7520492092826814 1101 34-35 0.8845589920067667 1101 36-37 0.872571508776737 1101 38-39 0.9074470624249784 1101 40-41 0.7897831449699666 1101 42-43 0.6495088469230126 1101 44-45 0.7631624161448798 1101 46-47 1.047579940254181 1101 48-49 0.9161238714770903 1101 50-51 1.0422380742232988 1101 52-53 1.1084717395324475 1101 54-55 1.2939805983166082 1101 56-57 1.106430227343715 1101 58-59 1.2084396837196394 1101 60-61 1.4211943990982405 1101 62-63 1.2723618141216448 1101 64-65 1.2850810465188118 1101 66-67 1.2959645914523463 1101 68-69 1.3126195740500357 1101 70-71 1.1936755208893608 1101 72-73 1.2007566012270097 1101 74-75 1.0758316122328608 1101 76-77 1.1113764080893134 1101 78-79 1.1245120498696295 1101 80-81 1.1887064666799745 1101 82-83 1.2922989212933516 1101 84-85 1.324469550609436 1101 86-87 1.297154083999569 1101 88-89 1.2382793925969295 1101 90-91 1.2124111673913553 1101 92-93 1.0503216246283529 1101 94-95 0.8980340343887292 1101 96-97 1.0056119415892724 1101 98-99 0.8320316870975937 1101 100-101 1.1307404566976302 1102 1 0.11477302200045614 1102 2 0.2015361203001227 1102 3 0.19716979580255156 1102 4 0.2336656573548339 1102 5 0.2768621767371684 1102 6 0.3005353597676361 1102 7 0.13571321846997364 1102 8 0.18478043721810167 1102 9 0.38345684377337363 1102 10-11 0.4541450821579218 1102 12-13 0.5054544380243371 1102 14-15 0.4957303498605796 1102 16-17 0.3740990292687343 1102 18-19 0.3362542040450549 1102 20-21 0.10029535161920933 1102 22-23 0.04438123775042868 1102 24-25 0.09987467585571608 1102 26-27 0.19980480971806003 1102 28-29 0.28818347223201357 1102 30-31 0.44045385823726235 1102 32-33 0.3254251818139977 1102 34-35 0.1491911227938374 1102 36-37 0.070371169783364 1102 38-39 -0.2069136745918101 1102 40-41 -0.2573644922337337 1102 42-43 0.07619175572314418 1102 44-45 -0.022374865811315203 1102 46-47 0.18221036576113647 1102 48-49 0.2530270204411025 1102 50-51 0.3991703496593786 1102 52-53 0.18143590250733865 1102 54-55 0.0791973307840621 1102 56-57 0.2729869262284339 1102 58-59 0.19750106732779926 1102 60-61 -0.19717134774849399 1102 62-63 0.06376900119375506 1102 64-65 0.09097843758415536 1102 66-67 0.03529537813811601 1102 68-69 0.016426449998458992 1102 70-71 0.03158588839280796 1102 72-73 -0.0990918423031566 1102 74-75 -0.022204725343527798 1102 76-77 0.05998487793499763 1102 78-79 0.05247643709725125 1102 80-81 0.15168557903730928 1102 82-83 0.028535500007379255 1102 84-85 0.08601119098336696 1102 86-87 0.10808205138483018 1102 88-89 -0.20539036060274896 1102 90-91 -0.06503203011197911 1102 92-93 0.15175712990332713 1102 94-95 0.19808490498099474 1102 96-97 -0.019948312480678965 1102 98-99 -0.19614644775768397 1102 100-101 0.12251949556722508 1103 1 0.2959643744305822 1103 2 0.4945540269766795 1103 3 0.46622287500023063 1103 4 0.4125076739615423 1103 5 0.4992372831610581 1103 6 0.21043735824794396 1103 7 0.3641782885495317 1103 8 -0.07721433542386791 1103 9 0.37954067375149236 1103 10-11 0.5263240482212979 1103 12-13 0.5416201342882943 1103 14-15 0.5947182930645596 1103 16-17 0.6223773654858107 1103 18-19 0.4737474524223728 1103 20-21 0.5083244041948944 1103 22-23 0.450937292239459 1103 24-25 0.522823670207238 1103 26-27 0.5508267078623987 1103 28-29 0.6133657660443177 1103 30-31 0.3485230772461634 1103 32-33 0.47592091112657187 1103 34-35 0.3808656167654405 1103 36-37 0.6428919510491014 1103 38-39 0.5297080422886182 1103 40-41 0.5514997017369581 1103 42-43 0.5956380311161098 1103 44-45 0.584813895826656 1103 46-47 0.5701024176136045 1103 48-49 0.8328774319467769 1103 50-51 0.20031068728628298 1103 52-53 0.5088041199874098 1103 54-55 0.5368948672428289 1103 56-57 0.5731322577674334 1103 58-59 0.5411815485010649 1103 60-61 0.6686642971573562 1103 62-63 0.6646687979584627 1103 64-65 0.46489222737295677 1103 66-67 0.4688145527465224 1103 68-69 0.23521713138595501 1103 70-71 0.45576001617470396 1103 72-73 0.39187128239359126 1103 74-75 0.415502286273842 1103 76-77 0.32867977782788316 1103 78-79 0.4523218363813477 1103 80-81 0.6843854041817217 1103 82-83 0.7348288133833734 1103 84-85 0.4632868311287446 1103 86-87 0.5194453756549677 1103 88-89 0.4852490295082994 1103 90-91 0.5065632066754375 1103 92-93 0.40615804354663965 1103 94-95 0.5005075931796164 1103 96-97 0.7959554208233612 1103 98-99 0.8828846142840057 1103 100-101 0.820809847383984 1104 1 0.24372419466879336 1104 2 0.3104012736017303 1104 3 0.24865835588067497 1104 4 0.3494705296665046 1104 5 0.3121569995682876 1104 6 0.3294719332561158 1104 7 0.32666118204331696 1104 8 0.31710122774438787 1104 9 0.3174687778650025 1104 10-11 0.35910828109933846 1104 12-13 0.36127970377823004 1104 14-15 0.2829097270091694 1104 16-17 0.18097708206963148 1104 18-19 0.2363391653685767 1104 20-21 0.2712632136703519 1104 22-23 0.2768218641574123 1104 24-25 0.16280974220024547 1104 26-27 0.06652076848343569 1104 28-29 0.20488811265965978 1104 30-31 0.30778420861530265 1104 32-33 0.1356166581180318 1104 34-35 -0.02408842743336237 1104 36-37 0.13883042501630172 1104 38-39 0.0997294971583429 1104 40-41 -0.0677338605660367 1104 42-43 0.02769341646499157 1104 44-45 -0.17669580301054566 1104 46-47 0.15470203707661767 1104 48-49 -0.07424946963452328 1104 50-51 -0.06543049900025011 1104 52-53 -0.0746647042327595 1104 54-55 0.29538212234387373 1104 56-57 0.3485079387167431 1104 58-59 0.3372962746645314 1104 60-61 0.2754969668318239 1104 62-63 0.0457687573443053 1104 64-65 -0.08933937044093909 1104 66-67 0.08669096210190474 1104 68-69 0.23102758246796995 1104 70-71 0.2345270895715501 1104 72-73 0.07045106713855986 1104 74-75 -0.3159132053781839 1104 76-77 -0.25069838411664946 1104 78-79 -0.0548965574170488 1104 80-81 0.09071856387481958 1104 82-83 -0.043147608332837706 1104 84-85 0.008190637381559895 1104 86-87 0.0502975666121408 1104 88-89 -0.15114975294991595 1104 90-91 -0.3207808261337348 1104 92-93 -0.13443470241181288 1104 94-95 -0.03284358242363794 1104 96-97 0.17054453358062105 1104 98-99 0.21681156177696437 1104 100-101 0.37461963186407843 1105 1 0.10196663289091745 1105 2 0.19430817489693908 1105 3 0.26020550086049 1105 4 0.3228192347888097 1105 5 0.43640584966114915 1105 6 0.3994309207468376 1105 7 0.36045405189133817 1105 8 0.09338789102679357 1105 9 0.3961372451597427 1105 10-11 0.3937582061001166 1105 12-13 0.24846509187547383 1105 14-15 0.4746686780698042 1105 16-17 0.43381926792743997 1105 18-19 0.24482404445907946 1105 20-21 -0.14869171623260513 1105 22-23 -0.24243381547309184 1105 24-25 -0.1848522051684327 1105 26-27 -0.10930035276037131 1105 28-29 0.01834349264613877 1105 30-31 5.26793924990443E-4 1105 32-33 -0.16682623023171317 1105 34-35 -0.1435639886264184 1105 36-37 -0.27717122359924673 1105 38-39 -0.42401841726360345 1105 40-41 -0.5502939514560126 1105 42-43 -0.421130147711267 1105 44-45 -0.24954582177689133 1105 46-47 -0.09730128661701087 1105 48-49 0.0415440511917069 1105 50-51 -0.08175611512510983 1105 52-53 0.14851012002296926 1105 54-55 0.08386150604922449 1105 56-57 0.07845342149690993 1105 58-59 -0.1334845728155969 1105 60-61 -0.006514423522673951 1105 62-63 -0.1320179784895359 1105 64-65 -0.05774972663981259 1105 66-67 -0.2377385388544937 1105 68-69 -0.11047245480009238 1105 70-71 -0.12752848879568646 1105 72-73 -0.03590911345937897 1105 74-75 -0.08140878018179976 1105 76-77 -0.0784628252699413 1105 78-79 -0.1660972858422589 1105 80-81 -0.23969654058380385 1105 82-83 -0.017848217897192598 1105 84-85 -0.13748051189048027 1105 86-87 -0.03698412303797127 1105 88-89 0.03053182608681837 1105 90-91 -0.003432036331339816 1105 92-93 0.0731067213692711 1105 94-95 0.14543072550405256 1105 96-97 0.08443171588142206 1105 98-99 -0.18785320870249578 1105 100-101 -0.20007824323895917 1106 1 0.04007488655996383 1106 2 0.06132401799195364 1106 3 -0.11440156345439689 1106 4 0.11878799741167256 1106 5 0.32175300643149285 1106 6 0.21403574099727507 1106 7 0.49990605135276667 1106 8 0.4555574525366026 1106 9 0.3880267474262453 1106 10-11 0.43753698622668225 1106 12-13 0.3703106104787679 1106 14-15 0.24183868839250522 1106 16-17 0.23890252271851153 1106 18-19 0.20101160336576385 1106 20-21 0.29041110680046245 1106 22-23 0.4737158187443953 1106 24-25 0.217767515669955 1106 26-27 0.13339633948863394 1106 28-29 -0.10788535704642754 1106 30-31 0.05708552109162213 1106 32-33 -0.026891308100751132 1106 34-35 -0.04132665637023081 1106 36-37 0.09705412535277702 1106 38-39 0.06212493357343618 1106 40-41 -0.13561395504650875 1106 42-43 -0.07992351425676247 1106 44-45 -0.08298933867469316 1106 46-47 -0.14260872344659248 1106 48-49 -0.27945859680433927 1106 50-51 -0.24232857596618373 1106 52-53 -0.10265365180055852 1106 54-55 0.24230816463725802 1106 56-57 0.07626792712951413 1106 58-59 0.06376016485327085 1106 60-61 0.11951784612411132 1106 62-63 0.0676180342567605 1106 64-65 -0.10203050398374103 1106 66-67 -0.015206112123202331 1106 68-69 0.01951856894210735 1106 70-71 0.02856100449456278 1106 72-73 0.12782366334597128 1106 74-75 0.11643220541130717 1106 76-77 0.017520748178288414 1106 78-79 0.11201410951701618 1106 80-81 0.006299150812449028 1106 82-83 0.23711767232317627 1106 84-85 0.20532636392299253 1106 86-87 0.02393773863789761 1106 88-89 0.12815334217676266 1106 90-91 0.3370865669629488 1106 92-93 0.29346711811986737 1106 94-95 0.2568081322631741 1106 96-97 0.46049495361761217 1106 98-99 0.8167210922714254 1106 100-101 0.8652123631072506 1107 1 0.42969104504935984 1107 2 0.4185370397120174 1107 3 0.5188420004933931 1107 4 0.3695417507006411 1107 5 0.2852488533076212 1107 6 0.39674584779188393 1107 7 0.5200221324547627 1107 8 0.5666341692471235 1107 9 0.703020814392147 1107 10-11 0.6361540733648923 1107 12-13 0.7254094083944622 1107 14-15 0.8556087446273466 1107 16-17 0.8144027032891188 1107 18-19 0.9530213541783397 1107 20-21 0.9318262644128055 1107 22-23 0.8481140027200524 1107 24-25 0.8396083039896141 1107 26-27 0.9750836427956528 1107 28-29 0.764668685155506 1107 30-31 0.7934805579796205 1107 32-33 0.9085278736564604 1107 34-35 0.8464822483400809 1107 36-37 0.8905873761395569 1107 38-39 1.0742009795931509 1107 40-41 0.9200479651928788 1107 42-43 0.8677097210525346 1107 44-45 0.720269444795349 1107 46-47 0.7402543524446514 1107 48-49 0.8922991290378874 1107 50-51 0.7773791576147886 1107 52-53 0.741203852197792 1107 54-55 0.9497554122253504 1107 56-57 0.9190494882913569 1107 58-59 1.1009793775654444 1107 60-61 0.9733265960596214 1107 62-63 1.1218733094979498 1107 64-65 0.9073803199341341 1107 66-67 0.9592671376317412 1107 68-69 0.9639833060829055 1107 70-71 1.091260792968999 1107 72-73 1.0325809248637974 1107 74-75 0.860249725918969 1107 76-77 1.0321222030614337 1107 78-79 1.1725800693787498 1107 80-81 1.2071689852034133 1107 82-83 1.200836652873143 1107 84-85 1.3164706656306748 1107 86-87 1.2189823653726641 1107 88-89 1.414369872125686 1107 90-91 1.2247414707446111 1107 92-93 1.315790803823834 1107 94-95 1.3227267207169753 1107 96-97 1.609282808954763 1107 98-99 1.87853763253867 1107 100-101 1.9700299867981172 1108 1 0.21720444779500525 1108 2 0.16172978940082672 1108 3 0.106194290192132 1108 4 0.1600023569921163 1108 5 0.3283645197027809 1108 6 0.30603012716952804 1108 7 0.443786979111664 1108 8 0.2202691283134257 1108 9 0.5066001457617944 1108 10-11 0.40731612642464654 1108 12-13 0.4425896275664698 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-2.807016625265746 2316 86-87 -2.942807330273549 2316 88-89 -3.2942271975607973 2316 90-91 -3.4047154467368124 2316 92-93 -3.537551285775489 2316 94-95 -3.6272968442161506 2316 96-97 -3.5525614959986314 2316 98-99 -3.907669433436258 2316 100-101 -4.98140231049182 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5987.0 3 2495.0 4 2555.0 5 2685.0 6 2997.0 7 3489.0 8 4387.0 9 5397.0 10 6511.0 11 7765.0 12 9592.0 13 11033.0 14 12271.0 15 13856.0 16 16333.0 17 19324.0 18 17691.0 19 13535.0 20 14037.0 21 15182.0 22 16646.0 23 19245.0 24 21621.0 25 25137.0 26 29204.0 27 34283.0 28 40608.0 29 47970.0 30 57459.0 31 69037.0 32 85057.0 33 106598.0 34 136990.0 35 180616.0 36 245274.0 37 309033.0 38 235685.0 39 35723.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.91612641470958 18.147208391394486 13.801065492981682 23.13559970091425 2 15.802377095012115 19.743970841477616 39.841112815635746 24.612539247874523 3 19.719387606091228 24.053841215356897 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28.03205389787048 22.602628253955697 48-49 22.778611119223285 26.76129590642033 27.851687051820857 22.60840592253553 50-51 22.530122049289638 26.932482484834434 27.881562993400138 22.65583247247579 52-53 22.801391485896776 27.00922126224008 27.566829792538194 22.62255745932495 54-55 22.531875775737905 26.85439689903958 27.70161220737673 22.912115117845786 56-57 22.678386958118164 27.143161701631698 27.42044293313843 22.758008407111717 58-59 22.888723868984577 26.82851066055426 27.528328982312456 22.7544364881487 60-61 22.694921545004032 26.78378853390495 28.05121198911066 22.47007793198036 62-63 22.51103513348622 26.9374338595542 27.999450964608457 22.552080042351125 64-65 22.409220604372045 27.099835630811565 27.745285370770535 22.745658394045858 66-67 22.384474733821897 27.21498705581782 27.39953225186822 23.001005958492065 68-69 22.358709507496137 27.01983614622636 27.787411185248967 22.834043161028532 70-71 22.45456840951531 27.002174832533115 27.63447201167628 22.90878474627529 72-73 22.526575083804637 26.924214482524615 27.653194027980366 22.89601640569038 74-75 22.317381890782908 27.386576787418676 27.410126283175384 22.885915038623033 76-77 22.570351646058466 27.107589107571584 27.402235949516573 22.919823296853377 78-79 22.515356437460547 27.196185011840836 27.397918764186898 22.890539786511724 80-81 22.49598359980987 27.266245508934333 27.32644504104031 22.91132585021549 82-83 22.551596241077604 27.25842657848589 27.446399248109323 22.743577932327184 84-85 22.476594707500553 27.257219126533418 27.267786389418507 22.998399776547526 86-87 22.455197851100507 27.313002121658574 27.28584085622504 22.945959171015875 88-89 22.468778981179373 27.23865132526544 27.112591077069066 23.17997861648612 90-91 22.47643793890114 27.138083130477636 27.367623380871663 23.017855549749562 92-93 22.52743853462891 27.1109848399358 27.40093650138271 22.960640124052578 94-95 22.372541601120428 26.99052815335497 27.487259138307547 23.149671107217056 96-97 22.279581448744675 27.035408849982318 27.523578819258965 23.16143088201404 98-99 22.225898553095305 27.052003381988364 27.237274525257632 23.4848235396587 100-101 22.189181896026025 26.929937510225503 27.43616630226693 23.444714291481546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 179.0 2 247.0 3 364.0 4 317.0 5 308.5 6 551.0 7 1043.0 8 1671.0 9 2112.0 10 1996.5 11 1770.0 12 1866.0 13 1935.0 14 1993.0 15 2199.0 16 2388.0 17 2521.5 18 2602.0 19 2821.0 20 3180.5 21 3641.5 22 4391.5 23 5370.5 24 6448.0 25 7804.5 26 9748.0 27 12158.5 28 14521.5 29 17193.0 30 20873.5 31 24782.5 32 28729.5 33 32052.5 34 35603.5 35 38838.5 36 42588.5 37 46485.5 38 48254.5 39 49725.5 40 51108.0 41 54270.0 42 57537.0 43 60877.5 44 64440.5 45 66484.0 46 67722.0 47 70306.0 48 77091.5 49 77424.5 50 71387.5 51 67657.5 52 65176.0 53 64600.0 54 63618.5 55 60169.0 56 55851.0 57 51087.5 58 46908.0 59 42114.0 60 35447.0 61 29081.0 62 23841.0 63 18888.5 64 14398.5 65 11160.5 66 8385.0 67 6622.5 68 4993.0 69 3326.0 70 2427.0 71 1800.5 72 1438.0 73 1224.5 74 950.5 75 732.0 76 530.5 77 297.5 78 163.5 79 120.0 80 88.5 81 55.5 82 39.5 83 22.0 84 16.0 85 17.0 86 9.5 87 5.0 88 6.5 89 5.0 90 5.0 91 4.0 92 3.0 93 4.5 94 4.0 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013539868541150845 2 0.0055221424638419135 3 0.00849560379052602 4 0.0360532185860448 5 0.04603555303991288 6 0.05713293549128749 7 0.024477958421453103 8 0.022513350044893955 9 0.012743405685789033 10-11 0.04306209171322877 12-13 0.0408054469563703 14-15 0.017787670436413855 16-17 0.0206018391920256 18-19 0.028486821460107566 20-21 0.022938130234420255 22-23 0.013725709874068605 24-25 0.0026283274226939876 26-27 0.0025221323753124125 28-29 0.0010884992356611466 30-31 0.005469044940151126 32-33 0.001300889330424297 34-35 0.001592925710723629 36-37 0.005548691225687307 38-39 0.006106215224440578 40-41 0.0036371803728189526 42-43 9.292066645887835E-4 44-45 0.0015398281870328413 46-47 0.0032654977069834393 48-49 6.371702842894516E-4 50-51 0.0016194744725690228 52-53 8.761091408979959E-4 54-55 0.0020973521857861116 56-57 0.002150449709476899 58-59 0.0027876199937663508 60-61 9.557554264341773E-4 62-63 0.0012743405685789033 64-65 0.0035309853254373775 66-67 0.0025752298990032 68-69 0.006557544175812273 70-71 0.010274370834167407 72-73 0.004141606847881435 74-75 0.003159302659601864 76-77 0.002946912564838714 78-79 0.03438064658978499 80-81 0.022407154997512384 82-83 0.0039557655149636784 84-85 0.008097372362845115 86-87 0.006929226841647786 88-89 0.005336301130924157 90-91 0.01141596759351934 92-93 0.013513319779305451 94-95 0.0060796664625951844 96-97 0.0036637291346643466 98-99 0.02182308223691372 100-101 0.04136297095512357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1883327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.74906821906312 #Duplication Level Percentage of deduplicated Percentage of total 1 81.60227022079968 50.38864150694583 2 10.949487226916563 13.522412674772625 3 3.16422223694356 5.861633243079131 4 1.3499015041254585 3.334206402690354 5 0.7158301099966642 2.210092114772174 6 0.4471670225021424 1.6567288186680074 7 0.29698475709533423 1.2836972418131178 8 0.22288597668487942 1.1010401103509706 9 0.17242772758604652 0.9582526362211937 >10 0.9718070504677265 11.093278975591586 >50 0.07057667122251692 2.9850436789540327 >100 0.034108724930072463 3.694529084848581 >500 0.0016401719947614724 0.6172161109725055 >1k 5.179490509773063E-4 0.6247320857239621 >5k 1.7264968365910213E-4 0.6684953145959223 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6710 0.356284383965185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5831 0.30961166064098267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3311 0.17580590094019785 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2796 0.14846067623944223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.0619504738157526E-4 0.0 12-13 7.964628553618145E-5 0.0 0.0 1.0619504738157526E-4 0.0 14-15 1.0619504738157526E-4 0.0 0.0 1.0619504738157526E-4 0.0 16-17 1.0619504738157526E-4 0.0 0.0 1.3274380922696907E-4 0.0 18-19 1.0619504738157526E-4 0.0 0.0 2.1239009476315052E-4 0.0 20-21 1.0619504738157526E-4 0.0 0.0 2.1239009476315052E-4 0.0 22-23 1.0619504738157526E-4 0.0 0.0 3.451339039901196E-4 0.0 24-25 1.592925710723629E-4 0.0 0.0 5.84072760598664E-4 0.0 26-27 1.592925710723629E-4 0.0 0.0 8.495603790526021E-4 0.0 28-29 1.592925710723629E-4 0.0 0.0 0.0015132794251874475 0.0 30-31 1.592925710723629E-4 0.0 0.0 0.0032124001832926517 0.0 32-33 1.592925710723629E-4 0.0 0.0 0.005681435034914277 0.0 34-35 1.592925710723629E-4 0.0 0.0 0.008893835218206927 0.0 36-37 1.592925710723629E-4 0.0 0.0 0.011628357688282492 0.0 38-39 1.592925710723629E-4 0.0 0.0 0.01425668511097648 0.0 40-41 1.592925710723629E-4 0.0 0.0 0.0184513894825487 0.0 42-43 1.8584133291775672E-4 0.0 0.0 0.024079726993772192 0.0 44-45 2.1239009476315052E-4 0.0 0.0 0.02984080831422265 0.0 46-47 2.6548761845393814E-4 0.0 0.0 0.036849681441406615 0.0 48-49 2.6548761845393814E-4 0.0 0.0 0.0425045677144755 0.0 50-51 2.6548761845393814E-4 0.0 0.0 0.05198247569328109 0.0 52-53 2.6548761845393814E-4 0.0 0.0 0.07526573983169146 0.0 54-55 2.6548761845393814E-4 0.0 0.0 0.10815965575813441 0.0 56-57 2.6548761845393814E-4 0.0 0.0 0.13069955456487375 0.0 58-59 2.6548761845393814E-4 0.0 0.0 0.16592976153371136 0.0 60-61 2.6548761845393814E-4 0.0 0.0 0.2077971589638974 0.0 62-63 2.6548761845393814E-4 0.0 0.0 0.2326998975748768 0.0 64-65 2.6548761845393814E-4 0.0 0.0 0.25396545581303726 0.0 66-67 2.6548761845393814E-4 0.0 0.0 0.32312498042028814 0.0 68-69 2.6548761845393814E-4 0.0 0.0 0.3902667991272891 0.0 70-71 2.6548761845393814E-4 0.0 0.0 0.4203730950599657 0.0 72-73 2.6548761845393814E-4 0.0 0.0 0.4787538223579867 0.0 74-75 2.6548761845393814E-4 0.0 0.0 0.5611346303642437 0.0 76-77 2.6548761845393814E-4 0.0 0.0 0.6415508299939416 0.0 78-79 2.6548761845393814E-4 0.0 0.0 0.7225245536223928 0.0 80-81 2.6548761845393814E-4 0.0 0.0 0.8370293634615762 0.0 82-83 2.6548761845393814E-4 0.0 0.0 0.9709944157334334 0.0 84-85 2.6548761845393814E-4 0.0 0.0 1.0973134245938172 0.0 86-87 2.6548761845393814E-4 0.0 0.0 1.3002521601400074 0.0 88-89 2.6548761845393814E-4 0.0 0.0 1.5637751702173865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1820 0.0 29.501745 2 GTACCTG 1935 0.0 28.485851 1 GTATCAA 11555 0.0 26.894283 1 GGTATCA 8415 0.0 26.539694 1 GTACATG 8550 0.0 24.675674 1 TACATGG 8690 0.0 23.511988 2 ATCAACG 13375 0.0 22.878773 3 TCAACGC 13405 0.0 22.863016 4 ACATGGG 8655 0.0 22.838467 3 GAGTACT 6870 0.0 22.615324 12-13 TATCAAC 13645 0.0 22.600176 2 CAACGCA 13485 0.0 22.588238 5 AACGCAG 13785 0.0 22.19948 6 GTGTAGC 895 0.0 21.767723 1 GTACTTT 7325 0.0 21.112974 14-15 ATAACGC 215 1.0760432E-6 19.890455 3 CATGGGA 4960 0.0 19.830303 4 ACGCAGA 15380 0.0 19.74068 7 CGCAGAG 15455 0.0 19.583395 8 GTATAGG 510 0.0 19.565966 1 >>END_MODULE