FastQCFastQC Report
Thu 26 May 2016
SRR936570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936570_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883327
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.389045556082401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69140.3671162787981057No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.2164786040873412No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34180.18148733597511213No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.10906231366087779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1252.1530104E-730.4034422
GTACCTG21100.027.0674421
GTATCAA122300.026.6848751
TACCTGG20500.026.6493592
GGTATCA88650.025.7697931
GTACATG87450.024.641621
TACATGG88500.023.7258512
ACATGGG86800.023.420583
GAGTACT82300.022.15885412-13
TCAACGC146050.022.0810014
TATCAAC146550.022.0751692
ATCAACG146250.022.0519733
CAACGCA147600.021.8163625
AACGCAG150700.021.4936476
GTACTTT87200.020.83199514-15
CATGGGG38500.020.601854
AGTACTT85600.020.5278712-13
ACGCAGA169750.019.1095267
TAACGCA1507.728713E-418.9981164
CGCAGAG171800.018.9091478
Produced by FastQC (version 0.11.5)