##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936570_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1883327 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.415342104690264 31.0 31.0 34.0 30.0 34.0 2 31.61778013058805 31.0 31.0 34.0 30.0 34.0 3 31.89357557131608 31.0 31.0 34.0 30.0 34.0 4 35.72130437252798 37.0 35.0 37.0 33.0 37.0 5 35.51736262475927 37.0 35.0 37.0 33.0 37.0 6 35.61895942658922 37.0 35.0 37.0 33.0 37.0 7 35.54837157859469 37.0 35.0 37.0 33.0 37.0 8 35.523827248268624 37.0 35.0 37.0 33.0 37.0 9 37.137485949067795 39.0 37.0 39.0 33.0 39.0 10-11 37.02167547112106 39.0 37.0 39.0 33.0 39.0 12-13 36.88846785502464 39.0 37.0 39.0 33.0 39.0 14-15 37.98678986708097 40.0 37.0 41.0 33.0 41.0 16-17 37.89917682909022 40.0 37.0 41.0 33.0 41.0 18-19 37.959317208323355 40.0 37.0 41.0 33.0 41.0 20-21 38.03339303264914 40.0 37.0 41.0 33.0 41.0 22-23 37.947195574639984 40.0 37.0 41.0 33.0 41.0 24-25 37.8464801917033 40.0 37.0 41.0 32.5 41.0 26-27 37.636058687630985 39.0 37.0 41.0 32.0 41.0 28-29 37.550589993134494 39.0 36.5 41.0 32.0 41.0 30-31 37.54276766594436 39.0 36.5 41.0 32.0 41.0 32-33 37.37238806643775 39.0 36.0 41.0 32.0 41.0 34-35 37.20476475938592 39.0 36.0 41.0 31.0 41.0 36-37 36.97719647198814 39.0 36.0 41.0 31.0 41.0 38-39 36.82726499434246 39.0 35.0 41.0 31.0 41.0 40-41 36.597263778409165 39.0 35.0 40.5 30.0 41.0 42-43 36.35979890905828 39.0 35.0 40.0 30.0 41.0 44-45 36.086099227590324 39.0 35.0 40.0 29.5 41.0 46-47 35.701613421354864 38.0 35.0 40.0 28.5 41.0 48-49 35.2599957415786 38.0 34.0 40.0 27.0 41.0 50-51 35.054068942886715 38.0 34.0 40.0 26.5 41.0 52-53 34.77031073201839 38.0 34.0 40.0 26.0 41.0 54-55 34.49925557271786 38.0 33.5 40.0 25.5 41.0 56-57 34.211865756716705 38.0 33.0 40.0 24.5 41.0 58-59 33.837658303629695 37.0 33.0 40.0 23.5 41.0 60-61 33.45713755497585 36.5 32.0 39.5 23.0 41.0 62-63 33.1567553059028 36.0 32.0 39.0 22.0 40.0 64-65 32.71248752871912 36.0 31.0 39.0 21.5 40.0 66-67 32.29526922302925 35.0 31.0 38.5 20.0 40.0 68-69 31.840843093100666 35.0 31.0 38.0 19.5 40.0 70-71 31.365696716502235 35.0 30.0 37.0 18.0 39.5 72-73 30.94430441447502 34.0 30.0 37.0 17.5 39.0 74-75 30.56670243669846 34.0 29.5 36.0 14.0 39.0 76-77 29.151731749186414 32.5 28.0 35.0 11.5 37.0 78-79 29.956471712028765 34.0 29.0 35.0 11.5 37.0 80-81 29.798195162072226 34.0 29.0 35.0 7.5 37.0 82-83 29.460129334948206 34.0 29.0 35.0 7.0 36.5 84-85 29.00619515357662 34.0 29.0 35.0 2.0 36.0 86-87 28.61034833568467 33.5 29.0 35.0 2.0 36.0 88-89 28.18966249621016 33.0 27.5 35.0 2.0 35.0 90-91 27.71337399187714 33.0 26.5 35.0 2.0 35.0 92-93 27.22780563332868 33.0 26.0 34.5 2.0 35.0 94-95 26.66907764822572 32.0 25.0 34.0 2.0 35.0 96-97 25.949117174022355 32.0 24.5 34.0 2.0 35.0 98-99 25.03770587901092 32.0 20.0 34.0 2.0 35.0 100-101 22.21033283120775 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3691520466072582 1101 2 0.42159954236507247 1101 3 0.3290217779510023 1101 4 0.2109860526077867 1101 5 0.24916481107859312 1101 6 0.2736703940723686 1101 7 0.21137885281005708 1101 8 0.28557385513195044 1101 9 0.35700595914963174 1101 10-11 0.3672340410572019 1101 12-13 0.2629081511316187 1101 14-15 0.42792400720803414 1101 16-17 0.35540294124312055 1101 18-19 0.33592540702448304 1101 20-21 0.20908215622605297 1101 22-23 0.0803659711033049 1101 24-25 0.08179641940615312 1101 26-27 0.1469127277524933 1101 28-29 0.23642932575140208 1101 30-31 -0.007163702825700113 1101 32-33 0.08352168414462824 1101 34-35 0.2874480912245616 1101 36-37 0.13046442602591668 1101 38-39 0.17933862700341763 1101 40-41 0.18172920767165834 1101 42-43 0.2417737843292045 1101 44-45 0.24628518512962927 1101 46-47 0.03578789029617724 1101 48-49 0.045054744468586705 1101 50-51 -0.015351447655334027 1101 52-53 -0.12535744466960352 1101 54-55 -0.18162443219636515 1101 56-57 -0.2857010537996487 1101 58-59 -0.1878869835383128 1101 60-61 -0.18971428597883744 1101 62-63 -0.23851518590284115 1101 64-65 -0.12602054076547375 1101 66-67 -0.2887061876285557 1101 68-69 -0.6039681830382229 1101 70-71 -0.5671777045425017 1101 72-73 -0.42027636790891165 1101 74-75 -0.26869151082741993 1101 76-77 -0.11864343486060491 1101 78-79 -0.1364321376355484 1101 80-81 -0.13058615868900247 1101 82-83 -0.08868563631487447 1101 84-85 -0.03553255287316048 1101 86-87 -0.2320495222488752 1101 88-89 -0.1381973909587444 1101 90-91 -0.1927609747487402 1101 92-93 -0.01673759062764546 1101 94-95 -0.20962782894125453 1101 96-97 -0.35345258539958024 1101 98-99 -0.4092924850431956 1101 100-101 -0.5116912009080039 1102 1 0.3961194565697852 1102 2 0.46752050515038945 1102 3 0.2888949300545498 1102 4 0.1314156680707228 1102 5 0.22067344193053628 1102 6 0.17062374686652504 1102 7 0.16654557571978756 1102 8 0.03848884080256454 1102 9 0.11518668670223065 1102 10-11 0.24645367993595357 1102 12-13 0.21996039578151994 1102 14-15 0.34539468586394406 1102 16-17 0.4357231933271706 1102 18-19 0.21284363728430833 1102 20-21 0.2131928166731214 1102 22-23 0.11373093966115988 1102 24-25 0.20254476155489698 1102 26-27 -0.017562617998443386 1102 28-29 0.05888196465438256 1102 30-31 0.16889255750700727 1102 32-33 0.2883213880830624 1102 34-35 0.38385865647814654 1102 36-37 0.30850790131481887 1102 38-39 0.27379123808466943 1102 40-41 0.3737891980347925 1102 42-43 0.31553542358548015 1102 44-45 0.12759840910917575 1102 46-47 0.33664930858556374 1102 48-49 0.37498351364728677 1102 50-51 0.34771455963635844 1102 52-53 0.1502260141613192 1102 54-55 0.5040639025604321 1102 56-57 0.4477335650174794 1102 58-59 0.2502855797759125 1102 60-61 0.3399340833440547 1102 62-63 0.35363577671698465 1102 64-65 0.30695175766669536 1102 66-67 0.44485554770862024 1102 68-69 0.4610738625496005 1102 70-71 0.23233591887073146 1102 72-73 0.20935310182572664 1102 74-75 -0.38584281522279795 1102 76-77 0.14784123932416549 1102 78-79 0.41331886988279365 1102 80-81 0.46086902086548065 1102 82-83 0.37593963497696237 1102 84-85 0.5257111071903999 1102 86-87 0.43599467976278206 1102 88-89 0.31878457984139175 1102 90-91 0.20020871419713515 1102 92-93 0.3907450873480727 1102 94-95 0.26359279760887944 1102 96-97 0.18795873895549065 1102 98-99 -0.30637451333358 1102 100-101 -0.24840986302821833 1103 1 0.5052151510943368 1103 2 0.5126792483873288 1103 3 0.24805058814322578 1103 4 0.12686896515127444 1103 5 -0.0028698096509458537 1103 6 -0.012746892042962088 1103 7 0.07199287644392882 1103 8 0.1873676137008644 1103 9 0.09665611443926991 1103 10-11 6.991918233865135E-4 1103 12-13 0.1535674559190241 1103 14-15 0.16305292559003703 1103 16-17 0.08211450822285116 1103 18-19 0.25315929280965577 1103 20-21 0.012729014525426408 1103 22-23 0.05201721761745404 1103 24-25 -0.07099471369524935 1103 26-27 0.10045769179804864 1103 28-29 0.2050088219227888 1103 30-31 0.1517258130250312 1103 32-33 0.109756073571873 1103 34-35 0.15045219963818113 1103 36-37 0.18386459297393998 1103 38-39 0.16056606803532247 1103 40-41 0.28825300475985927 1103 42-43 0.19432102409081864 1103 44-45 0.1987863742845306 1103 46-47 0.25441763409158824 1103 48-49 0.2516010422329771 1103 50-51 -0.03451024880065745 1103 52-53 0.059266665795888684 1103 54-55 0.19450211720252497 1103 56-57 0.21686285744743117 1103 58-59 0.2309206671579247 1103 60-61 0.11367713718309602 1103 62-63 -0.12427680464578827 1103 64-65 0.02454454098310066 1103 66-67 0.05534745491615212 1103 68-69 -0.12548617425246889 1103 70-71 -0.22749467261719047 1103 72-73 -0.3174010612934808 1103 74-75 -0.19521257985436336 1103 76-77 -0.23681850177971597 1103 78-79 0.07002357450502572 1103 80-81 0.004629148299496677 1103 82-83 0.0166084346986608 1103 84-85 0.07665896635383973 1103 86-87 0.04157129122635084 1103 88-89 0.15047520904685285 1103 90-91 -0.04666711545141666 1103 92-93 0.2673887069502072 1103 94-95 0.05926924154065816 1103 96-97 -0.16363967910498545 1103 98-99 -0.23650757751747875 1103 100-101 0.023790616680294363 1104 1 0.25812700954517 1104 2 0.34435542112110085 1104 3 0.13149987783694073 1104 4 0.009777027061375065 1104 5 -0.01108887888042176 1104 6 0.036825555666311516 1104 7 0.0863418138539842 1104 8 -0.05309092895724632 1104 9 0.047147015985260055 1104 10-11 -7.046453315098233E-4 1104 12-13 -0.01132955187713236 1104 14-15 0.11052634980490694 1104 16-17 -0.021003180125298115 1104 18-19 0.10615723561432588 1104 20-21 -0.14823360720668433 1104 22-23 0.042600692953179475 1104 24-25 0.14781747673216472 1104 26-27 0.14155425195555438 1104 28-29 -0.006095397534920721 1104 30-31 -0.12155029260708972 1104 32-33 0.059083953424604374 1104 34-35 0.06265002148330012 1104 36-37 0.03703569776728699 1104 38-39 -0.03357644350880662 1104 40-41 0.0011053987878213434 1104 42-43 0.008066819201225428 1104 44-45 0.10667810224031626 1104 46-47 0.22634762323171742 1104 48-49 0.30357115992573114 1104 50-51 0.14765141761584744 1104 52-53 0.19281406958007352 1104 54-55 0.13312099307329817 1104 56-57 0.17257047083597143 1104 58-59 0.22049905248032076 1104 60-61 -0.11797078222428325 1104 62-63 -0.25968065062984635 1104 64-65 -0.17020972129606804 1104 66-67 -0.02730599596279859 1104 68-69 -0.017559681461481347 1104 70-71 -0.09530464250973836 1104 72-73 0.05445410067754608 1104 74-75 -0.039326286249327325 1104 76-77 0.047793037055367193 1104 78-79 0.039333177987249 1104 80-81 0.05376918767757033 1104 82-83 0.14799103771293787 1104 84-85 0.08644664702061888 1104 86-87 0.16828613570061535 1104 88-89 -0.1248518011331754 1104 90-91 -0.27290529540744757 1104 92-93 -0.0057570581410395505 1104 94-95 0.0430268898044055 1104 96-97 -0.012064556158872364 1104 98-99 0.24341777189670566 1104 100-101 0.05329172474043986 1105 1 0.3370870056992814 1105 2 0.3603696614899512 1105 3 0.1329500244172941 1105 4 0.07282863296199338 1105 5 -0.10136535497130694 1105 6 -0.0345112474926097 1105 7 0.1392803233167612 1105 8 -0.004147599785881084 1105 9 -0.012885435895938713 1105 10-11 -0.01084651976945139 1105 12-13 0.0039231189638471164 1105 14-15 0.0759819441378653 1105 16-17 0.040679541210593584 1105 18-19 0.07178803394030808 1105 20-21 0.04003526708175542 1105 22-23 -0.04032757992136027 1105 24-25 -0.1270821089969445 1105 26-27 -0.13089600620846653 1105 28-29 -0.04404200232631439 1105 30-31 0.007827657137916333 1105 32-33 -0.006603981911553092 1105 34-35 -0.1592380623076508 1105 36-37 -0.009004140687792983 1105 38-39 -0.05193504341168875 1105 40-41 -0.08084298869593454 1105 42-43 0.14104025479165472 1105 44-45 -0.03734410070345717 1105 46-47 0.18402146991752488 1105 48-49 0.2501906159708085 1105 50-51 0.023441710013564432 1105 52-53 0.19420544729430134 1105 54-55 0.3642131854364834 1105 56-57 0.2357876880629064 1105 58-59 0.1629896924633485 1105 60-61 0.13362049784321073 1105 62-63 -0.060469520666636356 1105 64-65 0.19584853507909372 1105 66-67 0.29652501079970506 1105 68-69 0.06350805511462809 1105 70-71 -0.007322798450772439 1105 72-73 0.01635202852922646 1105 74-75 0.051842307845031854 1105 76-77 0.07380493107999442 1105 78-79 0.16235681299487936 1105 80-81 0.04016788889665435 1105 82-83 0.15988418025731832 1105 84-85 0.34282940897649894 1105 86-87 0.23628436006493203 1105 88-89 0.2059930988980092 1105 90-91 -0.1889147725211089 1105 92-93 0.048244032115146496 1105 94-95 0.05927146443469056 1105 96-97 0.05097006961907269 1105 98-99 0.06317365947859344 1105 100-101 0.17180823978416626 1106 1 0.2841100298005337 1106 2 0.3722075502741191 1106 3 0.11254295112615154 1106 4 -0.013733011488440638 1106 5 -0.044738632651842636 1106 6 -0.03329945268986734 1106 7 0.04397400851939892 1106 8 0.10686896140975932 1106 9 -0.0745478388401537 1106 10-11 -0.0747769986528013 1106 12-13 -0.05431214875303425 1106 14-15 0.008836842930556088 1106 16-17 0.08699546060380214 1106 18-19 -0.12269874852008655 1106 20-21 -0.12819416606757272 1106 22-23 -0.036527256776082595 1106 24-25 -0.1599592240276877 1106 26-27 -0.08724446453619095 1106 28-29 -0.012915706379097003 1106 30-31 -0.055408059391858444 1106 32-33 -0.08826279435264439 1106 34-35 -0.31305858203297987 1106 36-37 -0.2029570602156454 1106 38-39 -0.079065558200071 1106 40-41 0.004784900537039505 1106 42-43 -0.15919424994332587 1106 44-45 -0.13612377845580426 1106 46-47 0.021944588269477094 1106 48-49 -0.13938503144177616 1106 50-51 -0.06486289929481615 1106 52-53 0.06931383560721827 1106 54-55 -0.08348305799962219 1106 56-57 -0.1824632881051329 1106 58-59 -0.2319387218807023 1106 60-61 0.04786851184616836 1106 62-63 -0.5852246932351903 1106 64-65 -0.45025105650118036 1106 66-67 -0.37288480025006976 1106 68-69 -0.3211802443333305 1106 70-71 -0.408945705860674 1106 72-73 -0.18537275940668607 1106 74-75 -0.2347386355596619 1106 76-77 -0.0996419519144851 1106 78-79 -0.1584580068067467 1106 80-81 -0.32662422097273947 1106 82-83 -0.2697302894702531 1106 84-85 -0.08888236338560418 1106 86-87 -0.12452933770446961 1106 88-89 -0.07409127792529446 1106 90-91 -0.23976459972814723 1106 92-93 0.1603648974263976 1106 94-95 0.25353249401145916 1106 96-97 0.06087514569285801 1106 98-99 -0.24995321992538777 1106 100-101 0.03620301560212624 1107 1 0.6190860397061222 1107 2 0.6278088503824577 1107 3 0.3506211639931003 1107 4 0.14977315370786926 1107 5 -0.009893665412512576 1107 6 0.11141498779922188 1107 7 0.046855915344970356 1107 8 0.1083609905074212 1107 9 0.14609396067488944 1107 10-11 0.2089873825059385 1107 12-13 0.2327677666367336 1107 14-15 0.248780608085589 1107 16-17 0.36826022869955466 1107 18-19 0.42984626515168856 1107 20-21 0.2690616766812255 1107 22-23 0.39000501377027064 1107 24-25 0.394818416172491 1107 26-27 0.40124802888588107 1107 28-29 0.4832110541842809 1107 30-31 0.5074725576436876 1107 32-33 0.5255183540763824 1107 34-35 0.37786000642082485 1107 36-37 0.44965063725121013 1107 38-39 0.37104716944786276 1107 40-41 0.5668947390553356 1107 42-43 0.45919944556305126 1107 44-45 0.3804954239380507 1107 46-47 0.6274677271024842 1107 48-49 0.6879665137266642 1107 50-51 0.5624855503775805 1107 52-53 0.5640917337653946 1107 54-55 0.4892564560074888 1107 56-57 0.6111135575843889 1107 58-59 0.566503211772492 1107 60-61 0.442055170258179 1107 62-63 0.3306686153341758 1107 64-65 0.5031635336035478 1107 66-67 0.5744742257005058 1107 68-69 0.5417573814212027 1107 70-71 0.4447446413507983 1107 72-73 0.5939230152412023 1107 74-75 0.7550503547927043 1107 76-77 0.613083632360393 1107 78-79 0.6905191897056646 1107 80-81 0.700127829793395 1107 82-83 0.6597476035287677 1107 84-85 0.777918957359379 1107 86-87 1.003751630153964 1107 88-89 0.8688783346217832 1107 90-91 0.981981518003888 1107 92-93 1.0249320438949532 1107 94-95 1.0434803864945614 1107 96-97 1.0478670974031168 1107 98-99 1.0968991765044755 1107 100-101 0.9096003225205607 1108 1 0.36065309376862587 1108 2 0.34292109732685816 1108 3 0.09274337462543869 1108 4 0.1106556229725868 1108 5 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2316 70-71 -1.3609825136513791 2316 72-73 -1.275130125091259 2316 74-75 -1.3845347897232365 2316 76-77 -1.4198334184491088 2316 78-79 -1.2206206708324956 2316 80-81 -1.0611529822172727 2316 82-83 -1.0362830665602907 2316 84-85 -1.1115331845459586 2316 86-87 -1.0262971020025624 2316 88-89 -1.2297057039383361 2316 90-91 -1.230674478410986 2316 92-93 -1.394446937186128 2316 94-95 -1.6028725567436801 2316 96-97 -1.745480800498445 2316 98-99 -2.072664287021201 2316 100-101 -2.4253229396673284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 15.0 4 36.0 5 67.0 6 152.0 7 391.0 8 887.0 9 1618.0 10 2616.0 11 4026.0 12 5672.0 13 7819.0 14 9864.0 15 12663.0 16 15832.0 17 19586.0 18 17531.0 19 12528.0 20 11899.0 21 12401.0 22 13484.0 23 14979.0 24 17707.0 25 21436.0 26 26350.0 27 32839.0 28 40332.0 29 50160.0 30 61535.0 31 75257.0 32 92627.0 33 116196.0 34 148941.0 35 197092.0 36 273409.0 37 346469.0 38 207027.0 39 11883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27158748746746 19.322886017036208 14.52160156897498 22.883924926521352 2 16.019512599231795 20.686732611435136 38.99269870654153 24.30105608279154 3 19.916802912429066 24.39085001956735 29.79982891178443 25.89251815621915 4 13.325983761907873 16.71342459759238 36.183257102771336 33.77733453772842 5 13.669054816290533 37.470126005733476 33.89491044306167 14.96590873491433 6 32.37754250855003 35.91946592386771 16.882570047580693 14.820421520001572 7 29.02448698500048 32.77609252137308 19.936792707798485 18.262627785827952 8 27.121418638398957 34.632541242174085 19.044064041985273 19.201976077441678 9 26.550838840199546 16.493039975999004 20.086180575652264 36.869940608149186 10-11 25.507758883888865 26.59594069000305 26.504214620418676 21.392085805689405 12-13 26.39123293141024 23.84941915090926 27.38978840829269 22.369559509387805 14-15 22.90182976867249 25.283866224930318 26.05253313885465 25.761770867542545 16-17 22.21021628214325 28.62880423845673 26.73083325412953 22.43014622527049 18-19 22.130410704035995 26.83814334950861 29.259363881046678 21.772082065408714 20-21 22.943678925645944 25.793396473368674 29.48208144416769 21.780843156817696 22-23 22.553084501653885 25.87136531222281 29.485669925760416 22.08988026036289 24-25 22.533235067516156 26.04029995853083 29.59422341420263 21.83224155975038 26-27 22.356712350006134 26.17607032660818 29.56350649674751 21.90371082663818 28-29 22.038206854146942 26.475009384987313 29.550046274491898 21.936737486373847 30-31 22.251393411765456 26.096370943548308 29.470904415430777 22.18133122925546 32-33 22.111792205364786 26.298504070635566 29.48361975399895 22.1060839700007 34-35 22.661326441944304 26.23252650311299 29.221110737361457 21.885036317581246 36-37 22.701467790773773 26.001627486197563 29.191288037179962 22.1056166858487 38-39 22.377936692850838 26.23340303271977 29.40476051411174 21.98389976031765 40-41 22.524038978496407 26.161088568737924 29.265095034179062 22.0497774185866 42-43 22.373519779552765 26.2841056757516 28.952711009244958 22.38966353545068 44-45 22.532404141591915 26.36695285977617 28.736880308375973 22.36376269025594 46-47 22.80025497749035 26.706237204996437 28.235597714335476 22.257910103177743 48-49 22.974158107974276 26.808158462172845 27.953834172939708 22.26384925691318 50-51 22.732955400751813 26.914860035474653 27.99078115653954 22.361403407233993 52-53 23.02532123459006 27.031255558782785 27.632918295251745 22.310504911375414 54-55 22.686222066922294 26.91354576204221 27.73169376258069 22.668538408454808 56-57 22.869053889875364 27.201037781534882 27.576559221090374 22.353349107499383 58-59 23.196483632249016 26.829215842133014 27.490977307470317 22.483323218147653 60-61 22.872433070799243 26.867741017340848 28.092156687822328 22.167669224037585 62-63 22.73128055060179 26.947638614872123 28.032678640126356 22.28840219439973 64-65 22.654895314915613 27.070400274465424 27.778869455259425 22.495834955359538 66-67 22.659106374290758 27.161484032612588 27.496435039124968 22.68297455397169 68-69 22.633339413011093 26.99799052048148 27.7531980034232 22.615472063084223 70-71 22.720048977649967 27.015575784736217 27.659681793949797 22.60469344366402 72-73 22.888383931304528 26.818345093110512 27.629569275428345 22.66370170015661 74-75 22.62899645149249 27.360251299960126 27.3958531895948 22.614899058952588 76-77 22.84503723243831 27.17314889152256 27.33839040324876 22.64342347279037 78-79 22.775987142474417 27.180188832294842 27.347280374722633 22.69654365050811 80-81 22.743276144682483 27.363111156075338 27.290380638971712 22.60323206027047 82-83 22.820696443540587 27.360300917904755 27.33714685068839 22.48185578786626 84-85 22.74286199796351 27.28449918155424 27.190055983548778 22.782582836933475 86-87 22.704046952465514 27.250522543725864 27.198958523622803 22.84647198018582 88-89 22.750684524046648 27.343488987860486 27.010535429264966 22.895291058827905 90-91 22.790527836497095 27.22418445065276 27.19250257597807 22.792785136872073 92-93 22.78882414348629 27.14828006970839 27.29812549846703 22.764770288338287 94-95 22.629426049350528 27.09890018856793 27.35849307088529 22.913180691196253 96-97 22.63808123132903 27.00308294259981 27.369871066566443 22.98896475950472 98-99 22.46470204639979 27.07066812807735 27.053409462634193 23.411220362888667 100-101 22.31584682738448 27.208462644685326 27.159605788900148 23.316084739030043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 331.0 1 269.5 2 324.0 3 453.0 4 389.5 5 338.0 6 553.0 7 1120.0 8 1795.5 9 2204.5 10 2122.5 11 1859.5 12 1845.5 13 1999.5 14 2218.0 15 2389.5 16 2556.5 17 2773.0 18 3026.5 19 3261.5 20 3538.0 21 4059.0 22 4702.0 23 5561.0 24 6657.0 25 8010.5 26 9923.5 27 12432.5 28 14794.5 29 17462.5 30 21041.0 31 24891.5 32 28988.5 33 32299.5 34 36166.0 35 39523.0 36 42583.5 37 46304.5 38 48492.0 39 49962.5 40 51176.0 41 54262.0 42 57657.5 43 60816.5 44 64194.5 45 66307.5 46 67195.5 47 70218.5 48 77527.5 49 77481.0 50 71311.0 51 67498.0 52 64683.5 53 63691.5 54 62973.0 55 60059.5 56 55273.5 57 50183.0 58 46375.0 59 41890.5 60 35392.0 61 28756.5 62 23350.5 63 18607.0 64 14148.5 65 10885.0 66 8239.0 67 6510.5 68 4902.0 69 3209.0 70 2277.0 71 1735.5 72 1350.0 73 1156.0 74 945.5 75 721.0 76 489.5 77 285.5 78 178.0 79 127.5 80 87.5 81 53.0 82 34.5 83 21.5 84 10.5 85 7.5 86 5.5 87 4.0 88 3.5 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0184105043893068 2 0.01253101559102588 3 0.004672582084789312 4 0.01189384530673643 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0038230217057367093 10-11 1.0619504738157526E-4 12-13 3.451339039901196E-4 14-15 7.964628553618145E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001725669519950598 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004407094466335373 34-35 5.575239987532702E-4 36-37 0.0028938150411479263 38-39 5.309752369078763E-5 40-41 0.004433643228180767 42-43 0.013061990827933757 44-45 0.0022035472331676867 46-47 0.0012477918067335093 48-49 0.010752248547384496 50-51 5.575239987532702E-4 52-53 0.002442486089776231 54-55 0.012345174258108124 56-57 0.004194704371572223 58-59 0.0010619504738157526 60-61 0.0075929458877826315 62-63 0.0041681556097268296 64-65 0.02092042433417033 66-67 0.0035309853254373775 68-69 1.8584133291775672E-4 70-71 0.001008852950124965 72-73 2.6548761845393815E-5 74-75 0.0 76-77 0.0012212430448881155 78-79 0.012079686639654186 80-81 0.018504487006239488 82-83 0.01531863558479223 84-85 0.009796493120950319 86-87 0.012265527972571941 88-89 0.033557634972577785 90-91 0.029044345458860834 92-93 0.0028141687556117443 94-95 0.0098761394064865 96-97 0.011867296544891036 98-99 0.01141596759351934 100-101 0.0146018190149666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1883327.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.22363525151732 #Duplication Level Percentage of deduplicated Percentage of total 1 79.75125362212442 47.23159155367947 2 12.028166395820481 14.247034787412598 3 3.5078848790351564 6.232490837408733 4 1.500461314032812 3.5545109428516666 5 0.8081643033812098 2.393121396337268 6 0.4821286040701214 1.713204515506325 7 0.32667637135453004 1.3542873583672939 8 0.24977925231499215 1.1834268265999848 9 0.17700019184793325 0.9434335321305531 >10 1.0443748337605683 11.520112457579131 >50 0.08314675651992219 3.3478563833935144 >100 0.038083261411104255 3.981686856627359 >500 0.0020701540473433587 0.7765507123418028 >1k 6.300468839740654E-4 0.7615719403643145 >5k 1.8001339542116155E-4 0.7591198994000282 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7327 0.389045556082401 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6914 0.3671162787981057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.2164786040873412 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3418 0.18148733597511213 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2054 0.10906231366087779 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.309752369078763E-5 0.0 6 0.0 0.0 0.0 5.309752369078763E-5 0.0 7 0.0 0.0 0.0 1.0619504738157526E-4 0.0 8 0.0 0.0 0.0 1.0619504738157526E-4 0.0 9 0.0 0.0 0.0 1.0619504738157526E-4 0.0 10-11 0.0 0.0 0.0 1.592925710723629E-4 0.0 12-13 0.0 0.0 0.0 1.592925710723629E-4 0.0 14-15 0.0 0.0 0.0 1.592925710723629E-4 0.0 16-17 0.0 0.0 0.0 1.8584133291775672E-4 0.0 18-19 0.0 0.0 0.0 2.1239009476315052E-4 0.0 20-21 0.0 0.0 0.0 3.7168266583551343E-4 0.0 22-23 0.0 0.0 0.0 5.575239987532702E-4 0.0 24-25 0.0 0.0 0.0 7.964628553618145E-4 0.0 26-27 0.0 0.0 0.0 0.0010884992356611466 0.0 28-29 0.0 0.0 0.0 0.0016991207581052042 0.0 30-31 0.0 0.0 0.0 0.003371692754365015 0.0 32-33 2.6548761845393815E-5 0.0 0.0 0.005893825129677427 0.0 34-35 5.309752369078763E-5 0.0 0.0 0.009159322836660867 0.0 36-37 5.309752369078763E-5 0.0 0.0 0.012079686639654186 0.0 38-39 5.309752369078763E-5 0.0 0.0 0.014787660347884354 0.0 40-41 5.309752369078763E-5 0.0 0.0 0.019008913481301973 0.0 42-43 5.309752369078763E-5 0.0 0.0 0.02455760470698928 0.0 44-45 5.309752369078763E-5 0.0 0.0 0.030424881074821313 0.0 46-47 1.0619504738157526E-4 0.0 0.0 0.03756649801123225 0.0 48-49 1.0619504738157526E-4 0.0 0.0 0.04343377437906428 0.0 50-51 1.0619504738157526E-4 0.0 0.0 0.05301787740525145 0.0 52-53 1.0619504738157526E-4 0.0 0.0 0.07611530021074407 0.0 54-55 1.0619504738157526E-4 0.0 0.0 0.10919505747010477 0.0 56-57 1.0619504738157526E-4 0.0 0.0 0.1317880538005349 0.0 58-59 1.0619504738157526E-4 0.0 0.0 0.16712445581675406 0.0 60-61 1.0619504738157526E-4 0.0 0.0 0.2092042433417033 0.0 62-63 1.0619504738157526E-4 0.0 0.0 0.2340538844289919 0.0 64-65 1.0619504738157526E-4 0.0 0.0 0.2550805038105438 0.0 66-67 1.0619504738157526E-4 0.0 0.0 0.32421347965594927 0.0 68-69 1.0619504738157526E-4 0.0 0.0 0.39132874960110486 0.0 70-71 1.0619504738157526E-4 0.0 0.0 0.42183327696146233 0.0 72-73 1.3274380922696907E-4 0.0 0.0 0.48047949187793726 0.0 74-75 1.592925710723629E-4 0.0 0.0 0.5640284454053917 0.0 76-77 1.592925710723629E-4 0.0 0.0 0.6443649987495533 0.0 78-79 1.592925710723629E-4 5.309752369078763E-5 0.0 0.7255245637109222 5.309752369078763E-5 80-81 2.6548761845393814E-4 5.309752369078763E-5 0.0 0.8395780445987341 5.309752369078763E-5 82-83 2.6548761845393814E-4 5.309752369078763E-5 0.0 0.973596194394282 5.309752369078763E-5 84-85 2.6548761845393814E-4 5.309752369078763E-5 0.0 1.0997559106835935 5.309752369078763E-5 86-87 2.6548761845393814E-4 5.309752369078763E-5 0.0 1.302721194991629 5.309752369078763E-5 88-89 2.6548761845393814E-4 5.309752369078763E-5 0.0 1.5660583637360905 5.309752369078763E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 125 2.1530104E-7 30.403442 2 GTACCTG 2110 0.0 27.067442 1 GTATCAA 12230 0.0 26.684875 1 TACCTGG 2050 0.0 26.649359 2 GGTATCA 8865 0.0 25.769793 1 GTACATG 8745 0.0 24.64162 1 TACATGG 8850 0.0 23.725851 2 ACATGGG 8680 0.0 23.42058 3 GAGTACT 8230 0.0 22.158854 12-13 TCAACGC 14605 0.0 22.081001 4 TATCAAC 14655 0.0 22.075169 2 ATCAACG 14625 0.0 22.051973 3 CAACGCA 14760 0.0 21.816362 5 AACGCAG 15070 0.0 21.493647 6 GTACTTT 8720 0.0 20.831995 14-15 CATGGGG 3850 0.0 20.60185 4 AGTACTT 8560 0.0 20.52787 12-13 ACGCAGA 16975 0.0 19.109526 7 TAACGCA 150 7.728713E-4 18.998116 4 CGCAGAG 17180 0.0 18.909147 8 >>END_MODULE