FastQCFastQC Report
Thu 26 May 2016
SRR936569_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936569_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904679
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66830.35087277173738984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.3023606602477373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.16979239021378406No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29760.15624680064199795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19600.029.576622
GGTATCA73750.027.9622751
GTATCAA103950.027.7922571
GTACCTG21150.027.6337281
GTACATG78400.026.6068361
TACATGG79400.025.792982
CGTATAG1309.998548E-625.5858251
ACATGGG78950.024.5557273
ATCAACG124650.023.0244583
TCAACGC125450.022.954594
CAACGCA126200.022.7787255
TATCAAC128550.022.4737852
AACGCAG128950.022.366056
GAGTACT67500.022.34919512-13
TATCACG1100.002872246321.5984252
GTACTTT70650.021.35273614-15
CATGGGA45950.020.889764
ACGCAGA143250.020.0348957
AGTACTT71050.019.92847812-13
CGCAGAG144600.019.7816078
Produced by FastQC (version 0.11.5)