FastQCFastQC Report
Thu 26 May 2016
SRR936569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936569_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904679
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73650.3866793302178477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69650.36567841615306307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41450.21762197199633113No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35250.1850705551959149No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.10337699948390253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19600.030.0503062
GTACCTG20150.029.4743121
GGTATCA90750.027.0154271
GTACATG88400.026.1211781
TACATGG89000.025.403892
GTATCAA120100.024.5672931
ACATGGG89650.023.9998423
GAGTACT79750.021.44262112-13
ATCAACG146750.020.0342463
TCAACGC147100.020.0183414
TATCAAC147950.019.8727952
GTACTTT85800.019.65382814-15
CAACGCA149950.019.6378675
AACGCAG151950.019.4419026
CATGGGG40850.019.4166154
CATGGGA53300.018.9802324
AGTACTT84250.018.63406812-13
AGAGTAC133800.018.17690710-11
ACCTGGG32350.017.912083
TAAGGTG8250.017.8466555
Produced by FastQC (version 0.11.5)