##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936569_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1904679 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77489907748235 31.0 31.0 34.0 30.0 34.0 2 31.907123982571342 31.0 31.0 34.0 30.0 34.0 3 32.01443550330528 31.0 31.0 34.0 30.0 34.0 4 35.78612196595857 37.0 35.0 37.0 35.0 37.0 5 35.54870715747903 37.0 35.0 37.0 33.0 37.0 6 35.64884371592274 37.0 35.0 37.0 33.0 37.0 7 35.587262735610565 37.0 35.0 37.0 33.0 37.0 8 35.558402754479886 37.0 35.0 37.0 33.0 37.0 9 37.16871976852793 39.0 37.0 39.0 33.0 39.0 10-11 37.03548340691529 39.0 37.0 39.0 33.0 39.0 12-13 36.933960525631875 39.0 37.0 39.0 33.0 39.0 14-15 38.00404110088892 40.0 37.0 41.0 33.0 41.0 16-17 37.922380621616554 40.0 37.0 41.0 33.0 41.0 18-19 38.071638580569214 40.0 37.0 41.0 33.0 41.0 20-21 38.109405574377625 40.0 37.0 41.0 33.0 41.0 22-23 38.01553490115657 40.0 37.0 41.0 33.0 41.0 24-25 37.89749558849549 40.0 37.0 41.0 33.0 41.0 26-27 37.66958080600458 39.5 37.0 41.0 32.0 41.0 28-29 37.57982473687167 39.0 36.5 41.0 32.0 41.0 30-31 37.557302831605746 39.0 37.0 41.0 32.0 41.0 32-33 37.40524886345678 39.0 36.0 41.0 32.0 41.0 34-35 37.23500232847635 39.0 36.0 41.0 31.0 41.0 36-37 36.97805561987086 39.0 36.0 41.0 31.0 41.0 38-39 36.81465144520415 39.0 35.0 41.0 31.0 41.0 40-41 36.60162972343372 39.0 35.0 40.0 30.0 41.0 42-43 36.35046824163022 39.0 35.0 40.0 30.0 41.0 44-45 36.104710557527014 39.0 35.0 40.0 30.0 41.0 46-47 35.748542405308186 38.0 35.0 40.0 28.5 41.0 48-49 35.389964660711854 38.0 34.5 40.0 27.0 41.0 50-51 35.06283289730185 38.0 34.0 40.0 26.5 41.0 52-53 34.78506299486685 38.0 34.0 40.0 26.0 41.0 54-55 34.50438446583388 38.0 33.5 40.0 25.5 41.0 56-57 34.17107370848316 37.5 33.0 40.0 24.5 41.0 58-59 33.82569110070516 37.0 33.0 40.0 23.5 41.0 60-61 33.45502969266737 36.5 32.0 39.5 23.0 41.0 62-63 33.049041072012656 36.0 32.0 39.0 22.0 40.0 64-65 32.62237757648402 35.5 31.0 39.0 21.5 40.0 66-67 32.15237396957703 35.0 31.0 38.0 20.0 40.0 68-69 31.85599384463209 35.0 31.0 38.0 19.5 40.0 70-71 31.417146406297334 35.0 30.5 37.5 18.0 39.5 72-73 30.95806721237542 34.0 30.0 37.0 16.5 39.0 74-75 30.429247658004314 34.0 29.0 36.0 12.5 39.0 76-77 29.013232413440797 32.5 28.0 35.0 10.5 37.0 78-79 29.79876189111131 34.0 29.0 35.0 9.0 37.0 80-81 29.608550312152335 34.0 29.0 35.0 7.0 37.0 82-83 29.263155628848743 34.0 29.0 35.0 4.5 36.5 84-85 28.828382105331137 33.5 29.0 35.0 2.0 36.0 86-87 28.432086719074448 33.0 28.0 35.0 2.0 36.0 88-89 28.014150678408278 33.0 27.0 35.0 2.0 35.0 90-91 27.505628244969362 33.0 26.0 35.0 2.0 35.0 92-93 26.98058071727572 32.5 25.5 34.0 2.0 35.0 94-95 26.347101795105633 32.0 25.0 34.0 2.0 35.0 96-97 25.538203812820953 32.0 23.5 34.0 2.0 35.0 98-99 24.52982654820051 31.5 10.5 34.0 2.0 35.0 100-101 21.277636546630692 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3112928696424184 1101 2 0.37732151326554586 1101 3 0.32278797109763246 1101 4 0.16035161367410922 1101 5 0.09487519486842189 1101 6 0.21668406085455416 1101 7 0.18062138312042464 1101 8 0.19662755405773424 1101 9 0.3603915172966836 1101 10-11 0.2811675028740055 1101 12-13 0.2294219201768115 1101 14-15 0.4812983067915084 1101 16-17 0.44888447192524694 1101 18-19 0.45894884067006103 1101 20-21 0.30708724307545765 1101 22-23 0.28298635484499357 1101 24-25 0.3022806534635478 1101 26-27 0.4021163696809609 1101 28-29 0.28280484205380674 1101 30-31 0.2832051772845148 1101 32-33 0.36917684251318406 1101 34-35 0.3005092062753718 1101 36-37 0.3346859782406 1101 38-39 0.5177351068157918 1101 40-41 0.3624040199148695 1101 42-43 0.4182048822354716 1101 44-45 0.3510904440891309 1101 46-47 0.39870327913046566 1101 48-49 0.49358573522020066 1101 50-51 0.4350446900798275 1101 52-53 0.47451297684625615 1101 54-55 0.5628018974033964 1101 56-57 0.3842745968418697 1101 58-59 0.5341741623688776 1101 60-61 0.6018300337089144 1101 62-63 0.5445590551777499 1101 64-65 0.5060848602427086 1101 66-67 0.5559160131525402 1101 68-69 0.5607515936043832 1101 70-71 0.5166709978010893 1101 72-73 0.4198525029054814 1101 74-75 0.3453773175802475 1101 76-77 0.3016101492276455 1101 78-79 0.19483878621780804 1101 80-81 0.37638134192438955 1101 82-83 0.5434672965719543 1101 84-85 0.6211079142126437 1101 86-87 0.48787835537797264 1101 88-89 0.1330616811224523 1101 90-91 0.23323420797591865 1101 92-93 0.2127053955634537 1101 94-95 0.3991549636651186 1101 96-97 0.6760953331066801 1101 98-99 0.8183691807625628 1101 100-101 1.0237186765901463 1102 1 0.17125010190170542 1102 2 0.32555433181429905 1102 3 0.24615041868746346 1102 4 0.16663988513352024 1102 5 0.03478689864774509 1102 6 0.1814744367847041 1102 7 0.17415736356945644 1102 8 0.20580701207054375 1102 9 0.24179001672580114 1102 10-11 0.3221631762927828 1102 12-13 0.18743105243662228 1102 14-15 0.41428717228421164 1102 16-17 0.31606611291434916 1102 18-19 0.3164275742022795 1102 20-21 0.1675807323320555 1102 22-23 0.16537993000707019 1102 24-25 0.17281965222596085 1102 26-27 0.01775410756346929 1102 28-29 0.05388299137185015 1102 30-31 0.14772130555168417 1102 32-33 0.05984122722172458 1102 34-35 0.1063252347859347 1102 36-37 0.2834013332161689 1102 38-39 0.30074052725693434 1102 40-41 0.2560050342730804 1102 42-43 0.24869620369509704 1102 44-45 0.33459229952411107 1102 46-47 0.21848025219333778 1102 48-49 0.12778505679936814 1102 50-51 0.0381817816552541 1102 52-53 -0.08965476225534985 1102 54-55 -0.08567298390141787 1102 56-57 0.005601022951061907 1102 58-59 -0.20305646988310144 1102 60-61 0.0763714640090285 1102 62-63 0.09168990235945529 1102 64-65 0.06396575094637313 1102 66-67 0.03266781368106564 1102 68-69 -0.02636348605622274 1102 70-71 -0.02874067253628354 1102 72-73 0.01321897301573216 1102 74-75 -0.16485520592042846 1102 76-77 -0.45478046406915595 1102 78-79 -0.24135149322877325 1102 80-81 0.2232228095561446 1102 82-83 0.09905101029752217 1102 84-85 0.13253628370201298 1102 86-87 0.014693221247313204 1102 88-89 0.07563996491980518 1102 90-91 0.23436400019470938 1102 92-93 0.2718210292693328 1102 94-95 0.3407654470743573 1102 96-97 0.354746114296276 1102 98-99 0.36910938618623135 1102 100-101 0.6495133971269631 1103 1 0.19632203231940437 1103 2 0.20557564384546723 1103 3 0.1804295350706724 1103 4 0.1417330674573023 1103 5 -0.08520797684476378 1103 6 0.12454222227133727 1103 7 0.18123454891983926 1103 8 0.14464014136399328 1103 9 0.13549371751746264 1103 10-11 0.10709330862810162 1103 12-13 0.058253572889555016 1103 14-15 0.16455542267652845 1103 16-17 0.38926516996797744 1103 18-19 0.2512457351238382 1103 20-21 0.21739806768991343 1103 22-23 0.12837976026932552 1103 24-25 0.09393264622871556 1103 26-27 0.11313492830792171 1103 28-29 0.20869526465979504 1103 30-31 0.12437901203857393 1103 32-33 0.08009320684024601 1103 34-35 0.08423030559710298 1103 36-37 0.15280605784558077 1103 38-39 0.1770868089133728 1103 40-41 0.24445592988251263 1103 42-43 0.28869071843707417 1103 44-45 0.1516109600389015 1103 46-47 0.15438216782488468 1103 48-49 0.23153101165282663 1103 50-51 0.27565391020824137 1103 52-53 0.14217462679513204 1103 54-55 0.01560518296790292 1103 56-57 -0.01961728893071779 1103 58-59 0.07669989863558868 1103 60-61 -0.016166133827205442 1103 62-63 -0.17758423520397315 1103 64-65 -0.18039948768779368 1103 66-67 0.13766529536488292 1103 68-69 0.10869943022329309 1103 70-71 0.02284931228906828 1103 72-73 -0.06479668170716124 1103 74-75 -0.10370706649127115 1103 76-77 -0.30515802649633983 1103 78-79 -0.33370397516927497 1103 80-81 -0.16504986141763567 1103 82-83 0.10501692214766933 1103 84-85 -0.022598468182561504 1103 86-87 -0.3086087422068111 1103 88-89 -0.30890853498237547 1103 90-91 -0.2530059157087905 1103 92-93 -0.08411717879030078 1103 94-95 0.14179892796082072 1103 96-97 -0.09703876831411407 1103 98-99 0.2669004367056438 1103 100-101 0.5712956296084819 1104 1 0.18685621749204273 1104 2 0.2684876603390727 1104 3 0.12833401713039194 1104 4 0.07727657642153218 1104 5 0.08421201241908705 1104 6 0.09901877887187283 1104 7 0.1698598607913837 1104 8 0.08567731611447016 1104 9 0.18143902169139636 1104 10-11 0.250665519340167 1104 12-13 0.2326074628294279 1104 14-15 0.23834166983071725 1104 16-17 0.2187441067518776 1104 18-19 0.14700146866483976 1104 20-21 0.19485362353306357 1104 22-23 0.1284011425312741 1104 24-25 0.1303778114179437 1104 26-27 0.32583948059149037 1104 28-29 0.1295843841269999 1104 30-31 0.012156460954670933 1104 32-33 0.11147730316395155 1104 34-35 0.24257282017361348 1104 36-37 0.1913139360837235 1104 38-39 0.22994691152571534 1104 40-41 0.13509635113558716 1104 42-43 0.16712389380425918 1104 44-45 0.12363249535282961 1104 46-47 0.15202865601343518 1104 48-49 0.1765022975257935 1104 50-51 0.07373704669347347 1104 52-53 0.08472566569171391 1104 54-55 -0.08336036570656091 1104 56-57 -0.008266346366852417 1104 58-59 0.09192575721780116 1104 60-61 0.14646791326102715 1104 62-63 0.11963832150684794 1104 64-65 0.15977035499503245 1104 66-67 -0.15515634168360037 1104 68-69 0.15501948667246168 1104 70-71 0.18007263425979048 1104 72-73 0.027663482576134157 1104 74-75 0.15281986896918553 1104 76-77 0.18218513742570508 1104 78-79 0.28018256604728364 1104 80-81 0.37525412682436965 1104 82-83 0.3535644476009985 1104 84-85 0.3468815039529751 1104 86-87 0.23551929477775246 1104 88-89 0.27041132902644094 1104 90-91 0.45936676136165744 1104 92-93 0.568804257152383 1104 94-95 0.5656192179268018 1104 96-97 0.9268198971851547 1104 98-99 1.219348424353445 1104 100-101 1.3307066945914237 1105 1 0.027131750471340865 1105 2 0.1458776835383766 1105 3 0.17748245969836773 1105 4 0.05909425847011107 1105 5 -0.07630628864411904 1105 6 -0.023181086989964683 1105 7 0.09791892556252435 1105 8 0.1003313605999665 1105 9 -0.10978042614315342 1105 10-11 -0.03177117734266233 1105 12-13 -0.07548810511375592 1105 14-15 0.0954339502894328 1105 16-17 0.13523925248968993 1105 18-19 0.043038345664541566 1105 20-21 0.19087235889605836 1105 22-23 0.10311664868293491 1105 24-25 0.00669747805162757 1105 26-27 0.030015355513150155 1105 28-29 0.12227825225379263 1105 30-31 0.17238229684431872 1105 32-33 0.168293061099547 1105 34-35 0.16783752314554334 1105 36-37 -0.0033711141081340656 1105 38-39 0.04177086068516189 1105 40-41 0.1065133778862517 1105 42-43 0.092731638633893 1105 44-45 0.10043319023248642 1105 46-47 0.06610436711061851 1105 48-49 0.008623439679261935 1105 50-51 0.2435548606812219 1105 52-53 0.02735961144272636 1105 54-55 0.13498632646834352 1105 56-57 0.07047990161282769 1105 58-59 0.14686468037422884 1105 60-61 0.21875353958729704 1105 62-63 0.32867065759990766 1105 64-65 0.20970108692760903 1105 66-67 0.562869298496274 1105 68-69 0.48481465110166155 1105 70-71 0.36126800319471286 1105 72-73 0.3918997196708318 1105 74-75 0.014481642891407631 1105 76-77 -0.1563542958955324 1105 78-79 0.0841341443696777 1105 80-81 0.1658265229497573 1105 82-83 0.051254962273734606 1105 84-85 -0.11612440032515181 1105 86-87 0.05394954843081479 1105 88-89 0.008384523383842435 1105 90-91 0.1456670769161903 1105 92-93 0.29611126862009485 1105 94-95 0.3877036370096043 1105 96-97 0.6649893510669145 1105 98-99 0.6523277208977412 1105 100-101 0.8025492251848227 1106 1 0.17613942780234737 1106 2 0.21182264048170296 1106 3 0.1890792227208422 1106 4 0.09415978538137892 1106 5 0.077694473900074 1106 6 0.0716830199647589 1106 7 0.15912979159163143 1106 8 0.05958487947067681 1106 9 0.08359559161645791 1106 10-11 0.1705243513960042 1106 12-13 0.1731022033556755 1106 14-15 0.31976926217677004 1106 16-17 0.24414263272625902 1106 18-19 0.1902173833698697 1106 20-21 0.09077625125550526 1106 22-23 0.1963759159636993 1106 24-25 -0.007701183660763888 1106 26-27 0.14388937328629225 1106 28-29 0.22215033433784015 1106 30-31 0.18877113121205014 1106 32-33 0.13006917993010347 1106 34-35 0.23848306869176383 1106 36-37 0.2702976484270323 1106 38-39 0.41975860132086495 1106 40-41 0.3648307496806922 1106 42-43 0.31968543251684167 1106 44-45 0.2938502680638848 1106 46-47 0.24039850612377478 1106 48-49 0.17170400540582875 1106 50-51 0.26171830232741655 1106 52-53 0.16019480939964836 1106 54-55 0.13790215557722973 1106 56-57 0.28696608444746374 1106 58-59 0.22158216677590303 1106 60-61 0.21881704733569052 1106 62-63 0.11434272298919979 1106 64-65 0.3712583691118283 1106 66-67 0.3361275731159665 1106 68-69 0.2977912130200231 1106 70-71 0.3539401340093953 1106 72-73 0.22761081468880207 1106 74-75 0.24828716705029663 1106 76-77 -0.12447566224838269 1106 78-79 0.09926094554192133 1106 80-81 0.15546911984137424 1106 82-83 0.39598280393526863 1106 84-85 0.24842507818937776 1106 86-87 0.30551908095512914 1106 88-89 0.2809521089388163 1106 90-91 0.3870709704036557 1106 92-93 0.4190063195278171 1106 94-95 0.5641920082106999 1106 96-97 0.6622115920895375 1106 98-99 0.9547908739954067 1106 100-101 0.9912860188444235 1107 1 -0.004183267043245564 1107 2 0.05990524334952241 1107 3 -0.07096175519134817 1107 4 0.011378438396874913 1107 5 -0.047920742645985115 1107 6 0.026589468642846725 1107 7 -0.031820772617152215 1107 8 -0.19352297368028104 1107 9 -0.046711367692779504 1107 10-11 0.04251118821883182 1107 12-13 0.009857253355825435 1107 14-15 0.004571332173583187 1107 16-17 0.09040158520719643 1107 18-19 0.019212882306113954 1107 20-21 -0.004755285673752496 1107 22-23 -0.106657416488531 1107 24-25 -0.11710811999362392 1107 26-27 -0.08373673862194408 1107 28-29 -0.029794693176192766 1107 30-31 -0.13173180951456231 1107 32-33 -0.16730591120158067 1107 34-35 -0.16729924953567377 1107 36-37 -0.12283117489599249 1107 38-39 -0.06775124609752226 1107 40-41 -0.06951614196912459 1107 42-43 -0.40289435049316324 1107 44-45 -0.2925702464666884 1107 46-47 -0.05475553607909234 1107 48-49 -0.2420831032927282 1107 50-51 -0.06653605623541381 1107 52-53 -0.06553456603398189 1107 54-55 0.010821828431694769 1107 56-57 -0.32624132844735954 1107 58-59 -0.2450597387309017 1107 60-61 -0.13479705405448072 1107 62-63 -0.07914766748931612 1107 64-65 0.006579915139006687 1107 66-67 0.1686948015853318 1107 68-69 0.14958550471416743 1107 70-71 -0.023870093840717033 1107 72-73 -0.0881253768414787 1107 74-75 0.021199429630645028 1107 76-77 -0.06630681779703806 1107 78-79 -0.04014325486352277 1107 80-81 0.35262635343241655 1107 82-83 0.19156598686037185 1107 84-85 0.20643762911524632 1107 86-87 0.08047007261021122 1107 88-89 0.21080279417454406 1107 90-91 0.13882678741235566 1107 92-93 0.09549080867187953 1107 94-95 0.4199980144637969 1107 96-97 0.4983673813548961 1107 98-99 0.5138916507864302 1107 100-101 0.7704525075967332 1108 1 -0.026743379272470946 1108 2 0.052997611500487096 1108 3 0.027700704255998687 1108 4 -0.037182867438097844 1108 5 -0.03669988497073717 1108 6 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-0.9900953758283393 2316 98-99 -1.0985930179196721 2316 100-101 -1.3325990086163237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 18.0 4 30.0 5 74.0 6 158.0 7 370.0 8 877.0 9 1605.0 10 2579.0 11 4065.0 12 5707.0 13 7777.0 14 9963.0 15 12857.0 16 16047.0 17 19913.0 18 18081.0 19 13174.0 20 12251.0 21 12884.0 22 14080.0 23 15599.0 24 18585.0 25 22173.0 26 27490.0 27 33863.0 28 41903.0 29 51476.0 30 62607.0 31 76149.0 32 94050.0 33 118106.0 34 152800.0 35 201311.0 36 278862.0 37 349218.0 38 197758.0 39 10217.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.386891691250504 19.255746629909524 14.45051519698492 22.906846481855055 2 16.12150882133965 20.628210015194902 38.85694843918836 24.393332724277087 3 19.939472823413222 24.28159418334253 29.7597026985733 26.019230294670955 4 13.395044908112547 16.624638322604422 36.084260398274346 33.89605637100869 5 13.68547280304852 37.48182092150733 33.86049598301431 14.972210292429839 6 32.452674266225365 35.8395566270728 16.87602904857109 14.831740058130743 7 29.081278262636378 32.756963246825315 19.987829970299455 18.173928520238842 8 27.205844134365947 34.609138862769 19.002887100661056 19.182129902203993 9 26.55254246186963 16.53605649331898 20.104953666027882 36.8064473787835 10-11 25.52169142161109 26.53606661521418 26.522730971769388 21.419510991405335 12-13 26.374798454948152 23.77451945516814 27.467696351214204 22.382985738669507 14-15 23.006356096833606 25.217391532624745 25.999040232528348 25.7772121380133 16-17 22.256895285743873 28.616154485693052 26.684610786475915 22.44233944208716 18-19 22.14808899557353 26.838931914511576 29.292022435276493 21.720956654638393 20-21 22.930740560482896 25.79922916144925 29.42949966897309 21.84053060909476 22-23 22.523924255128353 25.87100984586726 29.436192448299092 22.1688734507053 24-25 22.571601980614922 26.014419363865798 29.53181694800282 21.882161707516456 26-27 22.414997989081183 26.182635532252934 29.460328194551934 21.942038284113952 28-29 22.042677236173397 26.470723357180002 29.56003588157819 21.926563525068406 30-31 22.266873073305423 26.067798647996266 29.47090929089764 22.194418987800674 32-33 22.12709366537266 26.2592706153389 29.492973600731677 22.120662118556773 34-35 22.692161583671407 26.177524032750227 29.178857953103027 21.951456430475343 36-37 22.68570478938112 26.04402375538496 29.133767516787263 22.13650393844666 38-39 22.42255395081898 26.226027291168897 29.32985496988636 22.021563788125768 40-41 22.566426159102512 26.123137947735874 29.212625400171422 22.09781049299019 42-43 22.390358844691043 26.304978085419613 28.900863562573637 22.40379950731571 44-45 22.5657639475537 26.372415342847226 28.696513223143533 22.36530748645554 46-47 22.83006515015407 26.687674603095434 28.187141441829773 22.29511880492073 48-49 22.99747435701897 26.746351783399007 27.948217624793042 22.307956234788982 50-51 22.72792424042754 26.88880465091615 27.944217777794112 22.439053330862198 52-53 23.04518862612243 26.9947010635938 27.62939209897998 22.330718211303786 54-55 22.759894550535044 26.86711550769738 27.69177295707413 22.681216984693446 56-57 22.858018911140636 27.185466894215693 27.530491910695144 22.426022283948527 58-59 23.14461710508527 26.818486691246633 27.504774455383945 22.53212174828415 60-61 22.906784339699765 26.843911278985424 28.060413751027447 22.188890630287364 62-63 22.75982106291151 26.94657089752071 27.971652542817836 22.32195549674994 64-65 22.66643563368671 27.061811823637825 27.709413018708425 22.562339523967047 66-67 22.696403917958204 27.14643829499355 27.493148876058648 22.6640089109896 68-69 22.623484208890403 26.98470452836687 27.746931664965537 22.64487959777719 70-71 22.77349621642282 26.98903594778963 27.594124784281238 22.643343051506317 72-73 22.8737494825196 26.84477005494152 27.645568517030455 22.63591194550843 74-75 22.671849333715226 27.390278088801388 27.306688943103985 22.631183634379397 76-77 22.831664932285634 27.205875403886765 27.302797726368226 22.659661937459376 78-79 22.752026142967253 27.15026325682399 27.318539706941706 22.77917089326705 80-81 22.7892225312135 27.293932088599902 27.261851600703462 22.65499377948314 82-83 22.82167338601174 27.30794867412712 27.292459658333314 22.577918281527825 84-85 22.7463441706514 27.250558002605324 27.224672772360663 22.778425054382613 86-87 22.767115167479652 27.226938716148158 27.12329515130307 22.882650965069125 88-89 22.732146410799395 27.303217628776828 27.016157191711947 22.94847876871183 90-91 22.801692394333656 27.207798771530705 27.140349794751895 22.850159039383744 92-93 22.792007684333303 27.115311464625215 27.273319124282118 22.819361726759368 94-95 22.638808966771535 27.147606466761033 27.256367634272653 22.957216932194775 96-97 22.67729359357596 27.049573453718867 27.271686761695076 23.001446191010093 98-99 22.48984829408287 27.08274047230766 27.013303538894657 23.41410769471481 100-101 22.362430992021604 27.149548155092422 27.110379424760467 23.37764142812551 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 346.0 1 280.0 2 319.5 3 428.0 4 359.0 5 320.0 6 548.5 7 1094.5 8 1723.5 9 2150.0 10 2046.5 11 1816.0 12 1887.5 13 2012.5 14 2217.5 15 2439.0 16 2613.0 17 2792.5 18 3003.0 19 3264.0 20 3612.0 21 4110.0 22 4686.5 23 5467.5 24 6483.0 25 8000.0 26 9998.5 27 12454.5 28 14799.5 29 17365.5 30 21106.5 31 25241.5 32 29252.5 33 32463.0 34 36168.5 35 39482.5 36 42929.0 37 46791.5 38 48791.0 39 50002.0 40 51446.0 41 54872.5 42 58406.5 43 61751.5 44 65053.0 45 66963.0 46 67883.5 47 70705.5 48 78658.0 49 78821.0 50 72158.5 51 68497.0 52 65577.0 53 64760.0 54 63946.5 55 60659.0 56 55866.0 57 51231.0 58 47569.5 59 42482.0 60 35545.0 61 29091.5 62 23902.0 63 18987.0 64 14475.0 65 11163.0 66 8464.5 67 6819.0 68 5069.5 69 3307.5 70 2366.5 71 1802.0 72 1447.5 73 1178.0 74 933.5 75 750.5 76 516.5 77 296.5 78 189.5 79 129.0 80 82.0 81 57.0 82 40.0 83 29.0 84 18.0 85 9.0 86 4.5 87 1.0 88 1.0 89 1.0 90 2.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034126485355275085 2 0.004882712520062436 3 0.004200182812956934 4 6.3002742194354E-4 5 8.925388477533484E-4 6 3.675159961337317E-4 7 0.0 8 0.0 9 0.0015225662696968885 10-11 4.725205664576551E-4 12-13 9.450411329153102E-4 14-15 0.0026776165432600453 16-17 0.0032813928226226043 18-19 0.0 20-21 0.0 22-23 0.0011550502735631568 24-25 9.450411329153102E-4 26-27 0.004042675957471049 28-29 0.004226433955537915 30-31 0.0012863059864680608 32-33 2.6251142580980836E-4 34-35 0.0074815756355795385 36-37 0.009686671612381929 38-39 0.0075340779207415005 40-41 0.0013913105567919845 42-43 5.775251367815784E-4 44-45 0.0013650594142110037 46-47 1.0500457032392334E-4 48-49 8.137854200104059E-4 50-51 0.0011550502735631568 52-53 0.0027038676858410263 54-55 0.003675159961337317 56-57 0.0018113288380876777 58-59 6.037762793625592E-4 60-61 0.004173931670375953 62-63 0.005197726231034206 64-65 0.009922931895610755 66-67 0.003386397392946528 68-69 0.0033076439652035853 70-71 0.0 72-73 9.187899903343292E-4 74-75 0.006589036787826191 76-77 0.003202639394879662 78-79 0.003937671387147126 80-81 0.0049614659478053776 82-83 0.005433986514263033 84-85 0.006195269649111478 86-87 0.003911420244566145 88-89 0.010605461602716259 90-91 0.014516881847282405 92-93 0.0012863059864680608 94-95 0.0024676074026121985 96-97 0.0010762968458202142 98-99 0.0038326668168232026 100-101 0.005171475088453225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1904679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.72850874694772 #Duplication Level Percentage of deduplicated Percentage of total 1 79.4974975457557 46.68769479976369 2 12.153199220095672 14.27478533401574 3 3.5808661144112666 6.308967807655523 4 1.5196422236082292 3.5698528648562786 5 0.786085532298701 2.3082815529726664 6 0.49237888839992167 1.7350006712524317 7 0.3430835522274888 1.4104149778548178 8 0.24063595240034433 1.1305752508298972 9 0.17630620505870445 0.9318780455338153 >10 1.0856983720352191 11.885257196861904 >50 0.08174304703208186 3.29707595824317 >100 0.03982176879598275 4.093666062933979 >500 0.002236454324008335 0.8456953476502956 >1k 6.262072107223336E-4 0.7679924577220612 >5k 1.7891634592066675E-4 0.7528616718536829 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7365 0.3866793302178477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6965 0.36567841615306307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4145 0.21762197199633113 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3525 0.1850705551959149 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.10337699948390253 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.6251142580980836E-4 0.0 12-13 0.0 0.0 0.0 2.887625683907892E-4 0.0 14-15 0.0 0.0 0.0 3.412648535527509E-4 0.0 16-17 0.0 0.0 0.0 3.675159961337317E-4 0.0 18-19 0.0 0.0 0.0 3.675159961337317E-4 0.0 20-21 0.0 0.0 0.0 4.7252056645765507E-4 0.0 22-23 0.0 0.0 0.0 6.562785645245209E-4 0.0 24-25 0.0 0.0 0.0 8.662877051723676E-4 0.0 26-27 0.0 0.0 0.0 0.0011025479884011953 0.0 28-29 5.250228516196168E-5 0.0 0.0 0.001601319697439831 0.0 30-31 5.250228516196168E-5 0.0 0.0 0.003045132539393777 0.0 32-33 5.250228516196168E-5 0.0 0.0 0.006247771934273439 0.0 34-35 7.875342774294252E-5 0.0 0.0 0.0087678816220476 0.0 36-37 1.0500457032392335E-4 0.0 0.0 0.0119180187317653 0.0 38-39 1.0500457032392335E-4 0.0 0.0 0.015435671837616731 0.0 40-41 1.0500457032392335E-4 0.0 0.0 0.019872114933802493 0.0 42-43 1.0500457032392335E-4 0.0 0.0 0.025463608303551412 0.0 44-45 1.0500457032392335E-4 0.0 0.0 0.032052645091377605 0.0 46-47 1.0500457032392335E-4 0.0 0.0 0.03950796958437616 0.0 48-49 1.5750685548588503E-4 0.0 0.0 0.04580824380381156 0.0 50-51 1.5750685548588503E-4 0.0 0.0 0.05502239484973583 0.0 52-53 1.5750685548588503E-4 0.0 0.0 0.07854341860229466 0.0 54-55 1.5750685548588503E-4 0.0 0.0 0.11140984911368268 0.0 56-57 1.5750685548588503E-4 0.0 0.0 0.1350621285791464 0.0 58-59 1.5750685548588503E-4 0.0 0.0 0.17107869620025212 0.0 60-61 1.5750685548588503E-4 0.0 0.0 0.21733320942794035 0.0 62-63 1.5750685548588503E-4 0.0 0.0 0.2434268451534353 0.0 64-65 1.5750685548588503E-4 0.0 0.0 0.26595032548791686 2.625114258098084E-5 66-67 1.5750685548588503E-4 0.0 0.0 0.33199820022166465 5.250228516196168E-5 68-69 1.5750685548588503E-4 0.0 0.0 0.40374257289548526 5.250228516196168E-5 70-71 1.8375799806686586E-4 0.0 0.0 0.43422014943200404 5.250228516196168E-5 72-73 2.100091406478467E-4 0.0 0.0 0.4924714348192005 5.250228516196168E-5 74-75 2.100091406478467E-4 0.0 0.0 0.5762125796525294 5.250228516196168E-5 76-77 2.6251142580980836E-4 0.0 0.0 0.6591661902084288 5.250228516196168E-5 78-79 2.6251142580980836E-4 0.0 0.0 0.7455849515850177 5.250228516196168E-5 80-81 2.6251142580980836E-4 0.0 0.0 0.8626125452110303 5.250228516196168E-5 82-83 2.6251142580980836E-4 0.0 0.0 0.9948395503914309 5.250228516196168E-5 84-85 2.6251142580980836E-4 0.0 0.0 1.1241001764601806 5.250228516196168E-5 86-87 2.6251142580980836E-4 0.0 0.0 1.3363931665125726 5.250228516196168E-5 88-89 2.6251142580980836E-4 0.0 0.0 1.5991146014630286 5.250228516196168E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1960 0.0 30.050306 2 GTACCTG 2015 0.0 29.474312 1 GGTATCA 9075 0.0 27.015427 1 GTACATG 8840 0.0 26.121178 1 TACATGG 8900 0.0 25.40389 2 GTATCAA 12010 0.0 24.567293 1 ACATGGG 8965 0.0 23.999842 3 GAGTACT 7975 0.0 21.442621 12-13 ATCAACG 14675 0.0 20.034246 3 TCAACGC 14710 0.0 20.018341 4 TATCAAC 14795 0.0 19.872795 2 GTACTTT 8580 0.0 19.653828 14-15 CAACGCA 14995 0.0 19.637867 5 AACGCAG 15195 0.0 19.441902 6 CATGGGG 4085 0.0 19.416615 4 CATGGGA 5330 0.0 18.980232 4 AGTACTT 8425 0.0 18.634068 12-13 AGAGTAC 13380 0.0 18.176907 10-11 ACCTGGG 3235 0.0 17.91208 3 TAAGGTG 825 0.0 17.846655 5 >>END_MODULE