FastQCFastQC Report
Thu 26 May 2016
SRR936568_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936568_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919658
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65380.34058149941291627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54820.285571700792537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.16596706288307606No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29550.15393366943486808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG19200.029.4541781
TACCTGG18950.029.08892
GTATCAA110450.028.31141
GTACATG87550.026.7060781
GGTATCA77900.026.1100371
TACATGG89200.025.6246972
ACATGGG86150.025.2651963
ATCAACG129250.024.0837023
TCAACGC130350.023.9181694
TATCAAC131700.023.705992
CAACGCA132450.023.5036755
GTATAGG4900.023.2764261
AACGCAG134050.023.1876836
GAGTACT66050.022.23532312-13
CATGGGA49100.020.810964
ACGCAGA148050.020.802387
CGCAGAG149000.020.5448348
GTACTTT71300.020.29413814-15
CATGGGG39950.019.8671154
GCAGAGT155250.019.352869
Produced by FastQC (version 0.11.5)