FastQCFastQC Report
Thu 26 May 2016
SRR936568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936568_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919658
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73800.38444347899469594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71820.37412914175337486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.21509039631017607No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36110.1881064231232855No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.10366429853651016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18750.028.1196772
GGTATCA94350.027.0395971
GTACCTG20450.026.4837421
GTATCAA127450.024.6766381
GTACATG86850.024.0685921
TACATGG88150.023.1165812
ACATGGG87750.021.6493533
GAGTACT82400.021.15184412-13
ATCAACG152100.020.3899423
TCAACGC152650.020.2837814
TATCAAC154350.020.1261022
CAACGCA154400.020.0538795
GTACTTT87300.019.82811414-15
AACGCAG157650.019.760446
AGTACTT86600.019.57761212-13
AGAGTAC136800.018.72900810-11
GTACACG2803.4850018E-818.6639021
ATAACGC2101.9592993E-518.0926723
CTACACT8350.017.6308544
ACGCAGA176450.017.6285977
Produced by FastQC (version 0.11.5)