FastQCFastQC Report
Thu 26 May 2016
SRR936567_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936567_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575560
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT243081.5428165223793444No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT177541.1268374419254106No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT136740.8678818959608012No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC115440.7326918682881007No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT84550.536634593414405No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT79200.50267841275483No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC67960.4313386986214425No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC63910.4056335525146615No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC61880.392749244712991No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG52680.3343573078778339No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA49440.31379319099240904No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA38860.24664246363197848No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT36830.23375815583030796No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36300.23039427251263042No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC34750.2205565005458377No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG29530.18742542334154205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.1846327654929041No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28490.18082459569930692No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC28450.1805707177130671No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC27150.17231968316027318No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.14763004899845136No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT21730.1379192160247785No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG20730.13157226636878316No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG18530.11760897712559346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG51450.025.3025281
GTATAGA3650.024.7320141
TACATGG51400.024.5884442
GTAAGCG2800.023.7538694-95
ACATGGG51400.023.3874973
GAGTACT39650.020.79035212-13
GTATAGC6550.019.5848921
GTGTAGG5700.019.1713051
CATGGGA23650.019.0873814
TAGCCCT9750.019.0070134
GAGTACG3506.366463E-1119.00461
GTGTAAG6550.018.8595261
GTACTTT43100.018.79634514-15
AGTACTT43350.018.2486512-13
AGAGTAC69900.018.2155610-11
TACGCAG2653.9928273E-717.9311454
TTTGCGT3751.70985E-1017.738753
GTGTAGC9000.016.8929771
CTTTGCG3702.73576E-916.693762
GTATATA9750.016.0808161
Produced by FastQC (version 0.11.5)