##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936567_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1575560 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.571929980451394 31.0 31.0 34.0 30.0 34.0 2 31.663652288710047 33.0 31.0 34.0 30.0 34.0 3 31.704020157912108 33.0 31.0 34.0 30.0 34.0 4 35.289522455507885 37.0 35.0 37.0 33.0 37.0 5 35.30050966005738 37.0 35.0 37.0 33.0 37.0 6 35.29488055040748 37.0 35.0 37.0 33.0 37.0 7 35.21893422021377 37.0 35.0 37.0 32.0 37.0 8 35.185758714361874 37.0 35.0 37.0 32.0 37.0 9 36.715558912386705 39.0 37.0 39.0 32.0 39.0 10-11 36.589031518952 39.0 37.0 39.0 32.0 39.0 12-13 36.52343515956231 39.0 37.0 39.0 32.0 39.0 14-15 37.619740282820075 40.0 37.0 41.0 32.0 41.0 16-17 37.54410400111706 40.0 37.0 41.0 32.0 41.0 18-19 37.50721203889411 40.0 37.0 41.0 32.0 41.0 20-21 37.335775851125945 40.0 37.0 41.0 32.0 41.0 22-23 37.47211277260149 40.0 37.0 41.0 32.0 41.0 24-25 37.49573675391606 40.0 37.0 41.0 32.0 41.0 26-27 37.39131705552312 40.0 37.0 41.0 32.0 41.0 28-29 37.28208002234126 40.0 37.0 41.0 31.0 41.0 30-31 37.128581583690874 39.0 36.5 41.0 31.0 41.0 32-33 36.932470994440074 39.0 36.0 41.0 30.5 41.0 34-35 36.69976167204042 39.0 36.0 41.0 30.0 41.0 36-37 36.47846733859707 39.0 35.5 40.5 30.0 41.0 38-39 36.26401565157785 39.0 35.0 40.0 30.0 41.0 40-41 36.12658610271903 39.0 35.0 40.0 29.5 41.0 42-43 35.890733136154765 38.0 35.0 40.0 29.0 41.0 44-45 35.62326664804895 38.0 35.0 40.0 27.5 41.0 46-47 35.48057611262027 38.0 35.0 40.0 27.0 41.0 48-49 35.31415623651273 38.0 35.0 40.0 26.5 41.0 50-51 34.35982095255021 37.5 33.5 39.5 25.0 40.5 52-53 34.519753294066874 38.0 33.5 39.5 26.0 40.5 54-55 34.965461486709486 38.0 34.0 40.0 26.0 41.0 56-57 34.754310530858874 38.0 34.0 40.0 25.5 41.0 58-59 34.49223165096855 38.0 34.0 40.0 25.0 41.0 60-61 34.086491469699666 37.5 33.0 40.0 23.0 41.0 62-63 33.78588819213486 37.0 33.0 40.0 23.0 41.0 64-65 33.433960623524335 36.5 32.5 39.5 22.0 41.0 66-67 32.862074119678084 36.0 32.0 39.0 20.5 41.0 68-69 32.408958402091955 35.5 31.5 39.0 19.5 40.0 70-71 31.903421323212065 35.0 31.0 38.0 18.0 40.0 72-73 31.517895859250046 35.0 31.0 37.0 17.0 39.5 74-75 31.080539300312267 35.0 30.5 37.0 13.5 39.0 76-77 30.61436568585138 34.5 30.0 36.0 9.0 39.0 78-79 30.055596422859175 34.0 29.5 36.0 7.0 38.5 80-81 29.476579438421894 34.0 29.0 35.0 2.0 37.0 82-83 29.003344207773743 34.0 28.5 35.0 2.0 37.0 84-85 28.60075941252634 34.0 28.0 35.0 2.0 36.0 86-87 28.319070679631366 34.0 27.5 35.0 2.0 36.0 88-89 27.847669400086318 33.5 26.5 35.0 2.0 35.5 90-91 27.35070165528447 33.0 26.0 35.0 2.0 35.0 92-93 26.797630366345935 33.0 25.0 35.0 2.0 35.0 94-95 26.24078264236208 33.0 24.5 35.0 2.0 35.0 96-97 25.61469763131839 32.0 21.5 35.0 2.0 35.0 98-99 24.829023331386935 32.0 10.5 34.5 2.0 35.0 100-101 21.86344061793902 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2858157139288551 1101 2 0.3371559859736841 1101 3 0.29149815400471013 1101 4 0.4066667982424761 1101 5 0.2740121990430211 1101 6 0.4210812528745791 1101 7 0.4870112169462715 1101 8 0.41654070778285046 1101 9 0.3256261921928143 1101 10-11 0.4878464173839916 1101 12-13 0.5789631062003124 1101 14-15 0.6382439558789699 1101 16-17 0.7268384480994854 1101 18-19 0.7572225768924667 1101 20-21 0.6583218491883684 1101 22-23 0.5820363483880016 1101 24-25 0.6382867901223079 1101 26-27 0.44253888278203135 1101 28-29 0.39544815066199845 1101 30-31 0.499874151235133 1101 32-33 0.4079174298866661 1101 34-35 0.3691889518577298 1101 36-37 0.35473422628754747 1101 38-39 0.3783891848934786 1101 40-41 0.3859635814427733 1101 42-43 0.38357606133666877 1101 44-45 0.31611972711925773 1101 46-47 0.43477293856532384 1101 48-49 0.5377685094017366 1101 50-51 0.27992963406895655 1101 52-53 0.4997110310872799 1101 54-55 0.4889766775789113 1101 56-57 0.5151294308227747 1101 58-59 0.43240828798823117 1101 60-61 0.48774639406367726 1101 62-63 0.4467118726797352 1101 64-65 0.3695861292565752 1101 66-67 0.6135676316638978 1101 68-69 0.4137176441840822 1101 70-71 0.43220970407951853 1101 72-73 0.45713780291773176 1101 74-75 0.3999322046274578 1101 76-77 0.4197801683404343 1101 78-79 0.4041414402427286 1101 80-81 0.5378199786258513 1101 82-83 0.1712755321124817 1101 84-85 0.17311294765511676 1101 86-87 0.40222284234199535 1101 88-89 0.5118120169413451 1101 90-91 0.27787942108883 1101 92-93 0.2112240335478539 1101 94-95 0.057787654367803754 1101 96-97 0.0028199428023718554 1101 98-99 -0.21030319081591387 1101 100-101 -0.0076609606736148805 1102 1 0.18744873960825714 1102 2 0.22543308931491524 1102 3 0.2035625707787787 1102 4 0.13165692421429043 1102 5 0.24802271748146865 1102 6 0.2222165928876052 1102 7 0.24552496782973776 1102 8 0.233344571472756 1102 9 0.23539533048464278 1102 10-11 0.2551103761417295 1102 12-13 0.40671588132191516 1102 14-15 0.24625413132486074 1102 16-17 0.18659593677034536 1102 18-19 0.14978015806737943 1102 20-21 0.14019846021401605 1102 22-23 0.1112571922882779 1102 24-25 0.10946913570636951 1102 26-27 -0.09848099173120062 1102 28-29 -0.2600565334383873 1102 30-31 -0.049368017101450334 1102 32-33 -0.06357261293543104 1102 34-35 -0.10486638947183025 1102 36-37 -0.29445021362986523 1102 38-39 -0.33811837133651323 1102 40-41 -0.28266987904032703 1102 42-43 -0.19669177600863463 1102 44-45 -0.25453479102816345 1102 46-47 -0.13239275628910008 1102 48-49 -0.03618904440305215 1102 50-51 0.014177527609611218 1102 52-53 0.052666570243872 1102 54-55 0.030474013323583904 1102 56-57 0.030345178338670564 1102 58-59 0.03973269485362607 1102 60-61 0.0737662858997652 1102 62-63 0.08600496155291637 1102 64-65 -0.07475553290615267 1102 66-67 0.012972071858463607 1102 68-69 -0.028580787058039903 1102 70-71 0.10051727832528456 1102 72-73 -0.16916920660291268 1102 74-75 -0.2795616041836375 1102 76-77 -0.1695011842974914 1102 78-79 -0.17736303251881225 1102 80-81 0.0839128516562404 1102 82-83 0.12288716406414224 1102 84-85 -0.2892351742007868 1102 86-87 -0.09247376514979422 1102 88-89 -0.08988332771420815 1102 90-91 -0.06402547816143667 1102 92-93 -0.010234395061189616 1102 94-95 -0.2350558838602801 1102 96-97 -0.18556265673476346 1102 98-99 -0.016269658962649203 1102 100-101 -0.06462197731741526 1103 1 0.17529975134797837 1103 2 0.06841064226164661 1103 3 0.27423502373140707 1103 4 0.47332924443138324 1103 5 0.3454653462349384 1103 6 0.309047753291118 1103 7 0.35079346231383823 1103 8 0.3942864038130267 1103 9 0.13100436534983828 1103 10-11 0.31007026051433684 1103 12-13 0.08174242842827795 1103 14-15 0.2918801268413489 1103 16-17 0.3478170667921674 1103 18-19 0.38092065125361785 1103 20-21 0.4465456152491143 1103 22-23 0.1729174556945594 1103 24-25 0.3234618573747099 1103 26-27 0.09456163849710464 1103 28-29 0.13990143364319607 1103 30-31 0.0611499833115019 1103 32-33 0.1325367611427879 1103 34-35 0.3087292317304602 1103 36-37 0.3653228086107134 1103 38-39 0.23140370700694746 1103 40-41 0.20654075012609496 1103 42-43 0.3604905067475457 1103 44-45 0.29053537444879396 1103 46-47 0.37076985211158586 1103 48-49 0.39678780799503954 1103 50-51 0.38119311666039835 1103 52-53 0.544660036706027 1103 54-55 0.531028921588586 1103 56-57 0.26715181953978373 1103 58-59 0.22556336118599773 1103 60-61 0.38524956859694726 1103 62-63 0.47481314396103613 1103 64-65 0.4915274297509242 1103 66-67 0.5252279859638307 1103 68-69 0.3953175080277944 1103 70-71 0.4720163786733451 1103 72-73 0.4393863358429577 1103 74-75 0.34170520898372203 1103 76-77 0.276771343871939 1103 78-79 0.24446126988222616 1103 80-81 0.23381827758981544 1103 82-83 0.28037602902785963 1103 84-85 0.39615304035882914 1103 86-87 0.4834067138779936 1103 88-89 0.242319602791369 1103 90-91 0.33787282429859644 1103 92-93 0.3051028269316127 1103 94-95 0.5463572702309243 1103 96-97 0.5667528408759992 1103 98-99 0.41573564308443167 1103 100-101 0.3233086757413979 1104 1 0.137360812714018 1104 2 0.16085025465222458 1104 3 0.08777390224869208 1104 4 0.16247235688023665 1104 5 0.20761591805902668 1104 6 0.2884455232487326 1104 7 0.24138366902113972 1104 8 0.26523568496890704 1104 9 0.10676701898986352 1104 10-11 0.38428087131202915 1104 12-13 0.28173759762400863 1104 14-15 0.05897925698985773 1104 16-17 0.06052351338545492 1104 18-19 0.1215890198629026 1104 20-21 -0.04779799858098244 1104 22-23 0.10493014767430253 1104 24-25 0.13275154511561027 1104 26-27 -0.17286210125084978 1104 28-29 -0.10182817831797308 1104 30-31 0.05258386825963157 1104 32-33 -0.030251119008276817 1104 34-35 -0.09187147494720449 1104 36-37 -0.16025118806446415 1104 38-39 -0.1392764293393114 1104 40-41 -0.10066760220669835 1104 42-43 0.09393158320408901 1104 44-45 0.038426690042939526 1104 46-47 -0.12305004119154006 1104 48-49 -0.15141224455593516 1104 50-51 -0.1897923571878195 1104 52-53 -0.011819988812888482 1104 54-55 -0.10119747597076412 1104 56-57 -0.09772176674707111 1104 58-59 0.04057589060533218 1104 60-61 -0.12046419934858932 1104 62-63 -0.0805561799717438 1104 64-65 -0.20233542959446282 1104 66-67 -0.2012327061140624 1104 68-69 -0.05452404670393918 1104 70-71 -0.0778954144189612 1104 72-73 -0.20337913857780165 1104 74-75 -0.08066657789243692 1104 76-77 -0.4078529217946958 1104 78-79 -0.22160999802753523 1104 80-81 0.010138109549391316 1104 82-83 -0.05575861698920548 1104 84-85 -0.24872546363566173 1104 86-87 -0.265269415712293 1104 88-89 -0.28048984472820493 1104 90-91 -0.5715642924320399 1104 92-93 -0.7429310868479853 1104 94-95 -0.580949886107188 1104 96-97 -0.35293260986615493 1104 98-99 -0.5509010078442103 1104 100-101 -0.4660415478558271 1105 1 0.18508319391898453 1105 2 0.15795009290456363 1105 3 0.11703909637862608 1105 4 0.2793323130926808 1105 5 0.17970134963714912 1105 6 0.25660950176095554 1105 7 0.26039720474641825 1105 8 0.28250541283556174 1105 9 0.3510807483321159 1105 10-11 0.29367939812044597 1105 12-13 0.17902128468484335 1105 14-15 0.18814082129274112 1105 16-17 0.17271262390428888 1105 18-19 0.020883502106677554 1105 20-21 0.0543667406472963 1105 22-23 -0.24446321674251692 1105 24-25 -0.07455831407006741 1105 26-27 -0.2004582494300493 1105 28-29 -0.32036023644236167 1105 30-31 -0.3144931430539657 1105 32-33 -0.3248178109957678 1105 34-35 -0.2351180189825115 1105 36-37 -0.39693620141246555 1105 38-39 -0.3956924471599237 1105 40-41 -0.3512165838076271 1105 42-43 -0.2932865316607547 1105 44-45 -0.19799883945690766 1105 46-47 -0.3103134770523681 1105 48-49 -0.3987699295343603 1105 50-51 -0.3393377283888839 1105 52-53 -0.26076036155284044 1105 54-55 -0.20720822422286034 1105 56-57 -0.2075171319453446 1105 58-59 -0.23738547222239248 1105 60-61 -0.21836778268138346 1105 62-63 -0.24921372350281956 1105 64-65 -0.26002774778114457 1105 66-67 -0.11104392411105124 1105 68-69 -0.18539954138766035 1105 70-71 -0.1810987711740566 1105 72-73 -0.31401066387352117 1105 74-75 -0.22397915251791645 1105 76-77 -0.21324508347250415 1105 78-79 -0.007270063818012318 1105 80-81 0.13203753677494845 1105 82-83 0.008347126908148539 1105 84-85 -0.0739223002792535 1105 86-87 -0.29663885741001295 1105 88-89 -0.1919845699208942 1105 90-91 -0.22825870286072458 1105 92-93 -0.4101985773144783 1105 94-95 -0.17538686345596943 1105 96-97 -0.2390688598961681 1105 98-99 0.07809534355640224 1105 100-101 0.24201729467704425 1106 1 0.30156870538330693 1106 2 0.2470747659714867 1106 3 0.33936911058129837 1106 4 0.2850314906829894 1106 5 0.2587260219279699 1106 6 0.3113124227511648 1106 7 0.5391614947292638 1106 8 0.5400216398402975 1106 9 0.6010432367229157 1106 10-11 0.5477657382032817 1106 12-13 0.41867002080231686 1106 14-15 0.46001623359707366 1106 16-17 0.5934594865109091 1106 18-19 0.45400531523677046 1106 20-21 0.3843811050066108 1106 22-23 0.4647269011474151 1106 24-25 0.32828791161529125 1106 26-27 0.41773339350083205 1106 28-29 0.44107854073092767 1106 30-31 0.5027659806748375 1106 32-33 0.4497970805956868 1106 34-35 0.4142874866869235 1106 36-37 0.31366723102912175 1106 38-39 0.3077257335528927 1106 40-41 0.3566734011101147 1106 42-43 0.40360737774741295 1106 44-45 0.2654907232612089 1106 46-47 0.3457049049296401 1106 48-49 0.31695351418658646 1106 50-51 -0.26979605557807673 1106 52-53 0.05864306804303965 1106 54-55 0.3074905058151458 1106 56-57 0.2087897789865636 1106 58-59 0.21678261729842063 1106 60-61 0.5318994875341474 1106 62-63 0.6265715949707413 1106 64-65 0.47675118854484566 1106 66-67 0.631623526418899 1106 68-69 0.6379335526474819 1106 70-71 0.48032758836308176 1106 72-73 0.26179350741741914 1106 74-75 0.5980890565226389 1106 76-77 0.587729151225993 1106 78-79 0.5253588920398755 1106 80-81 0.6864706005420977 1106 82-83 0.7619280303946603 1106 84-85 0.7473620454794734 1106 86-87 0.6769167516103671 1106 88-89 0.6616764984614782 1106 90-91 0.6713149582982005 1106 92-93 0.6935358974065764 1106 94-95 0.8042331762804658 1106 96-97 0.9175857642504432 1106 98-99 0.8891834841964794 1106 100-101 0.887858874096878 1107 1 0.34883378134418663 1107 2 0.39828773360389746 1107 3 0.3758580136063294 1107 4 0.35842047839098967 1107 5 0.45789022034831817 1107 6 0.353852561347864 1107 7 0.459042420026762 1107 8 0.58289298781866 1107 9 0.6742795832825905 1107 10-11 0.6710873702658091 1107 12-13 0.4313113005924478 1107 14-15 0.29920659281135187 1107 16-17 0.4464350212022623 1107 18-19 0.4535085011642863 1107 20-21 0.5256917520188367 1107 22-23 0.49618211539460333 1107 24-25 0.5647073227491148 1107 26-27 0.4388378581083501 1107 28-29 0.6608757690159877 1107 30-31 0.6045064184189783 1107 32-33 0.45181501118339895 1107 34-35 0.49542721213004626 1107 36-37 0.5687346067589658 1107 38-39 0.5590743984358255 1107 40-41 0.6958911480902543 1107 42-43 0.7040188515415267 1107 44-45 0.6544424620009153 1107 46-47 0.7431979749119577 1107 48-49 0.7096938938686677 1107 50-51 0.7223390909957743 1107 52-53 0.7297539779047852 1107 54-55 0.8935857736108943 1107 56-57 0.7653210490595157 1107 58-59 0.8522520016104878 1107 60-61 0.8949922455150272 1107 62-63 0.9174433621645122 1107 64-65 0.8374212247587707 1107 66-67 0.8024289713988821 1107 68-69 0.9399700338819272 1107 70-71 0.8906333855497337 1107 72-73 0.7282182975084588 1107 74-75 0.935727624923949 1107 76-77 0.887521113756609 1107 78-79 0.7091351829257029 1107 80-81 0.8490756006717355 1107 82-83 0.6758190687159882 1107 84-85 0.7205919650434325 1107 86-87 0.7398330228281225 1107 88-89 0.5249722910798518 1107 90-91 0.5324387184996873 1107 92-93 0.6540016574042973 1107 94-95 0.7460330783418136 1107 96-97 0.7371058648621407 1107 98-99 0.8225287829404024 1107 100-101 0.6243164455177812 1108 1 0.414197682061765 1108 2 0.36648085849043 1108 3 0.4979620639546134 1108 4 0.44324903052763887 1108 5 0.3303816447701635 1108 6 0.2242418941885873 1108 7 0.4258341505258798 1108 8 0.2788190706346967 1108 9 0.4332887178260094 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2316 88-89 -3.227766717104 2316 90-91 -3.64429893056705 2316 92-93 -4.311120282461296 2316 94-95 -3.964848472687507 2316 96-97 -4.221646844997249 2316 98-99 -5.223546766394808 2316 100-101 -6.053874945493337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3464.0 3 1317.0 4 1325.0 5 1463.0 6 1660.0 7 1944.0 8 2462.0 9 3159.0 10 3722.0 11 4662.0 12 5834.0 13 6895.0 14 7890.0 15 9335.0 16 11108.0 17 13225.0 18 11413.0 19 9979.0 20 10579.0 21 11922.0 22 12963.0 23 14964.0 24 17052.0 25 19226.0 26 22688.0 27 26452.0 28 31289.0 29 37472.0 30 45844.0 31 56363.0 32 70093.0 33 89891.0 34 118989.0 35 162096.0 36 221095.0 37 276119.0 38 202095.0 39 27508.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.73059456961529 18.360334603309216 14.660794562251883 22.248276264823616 2 15.153119390213869 20.663373476232916 38.873865982129935 25.309641151423286 3 21.95039427360595 23.038947452615435 29.57229788319954 25.438360390579078 4 12.340390414219964 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23.329923940821917 26.252967615425604 27.707698171336414 22.70941027241607 50-51 21.077405558972444 26.93202886435922 29.542585339195572 22.447980237472763 52-53 22.671936934506714 27.93393229472455 26.525526735871992 22.868604034896748 54-55 21.76579593538844 26.127682640775664 27.827517579602105 24.27900384423379 56-57 23.200801752278092 25.731475187379125 27.235744492872012 23.83197856747077 58-59 24.71630651971351 25.87110623636913 26.73256454590001 22.680022698017353 60-61 23.051839759418883 26.236473897323325 29.116033864407175 21.595652478850614 62-63 21.953568668768575 26.885838459841864 28.48448401433783 22.676108857051734 64-65 21.460691772005678 28.383108607290925 27.388692696128587 22.767506924574814 66-67 22.500863336388868 26.500332638432184 26.44913552096003 24.54966850421892 68-69 21.936871581099574 26.565336516634545 27.582109169875896 23.915682732389985 70-71 23.609856899181683 26.209885857188763 26.838007892327013 23.342249351302538 72-73 22.860594334190942 25.344551449906017 29.037287287763863 22.757566928139177 74-75 21.77689906770385 28.338399884193123 27.24420267219289 22.640498375910138 76-77 22.1728753096174 26.292092174008552 27.095137256968393 24.439895259405656 78-79 22.558422951814958 26.451984519616573 27.66013521838035 23.329457310188122 80-81 23.739736278088262 26.245145257947676 26.88262990127262 23.132488562691442 82-83 22.83722423257573 25.9084453580202 28.52839265807681 22.725937751327262 84-85 22.461704491350577 27.018314553245514 27.666560863355024 22.853420092048882 86-87 22.22911358614764 27.415686684033325 27.356330668237643 22.998869061581388 88-89 21.889208567325674 27.321301376211437 26.040982888456917 24.748507168005972 90-91 22.44189269288172 25.78291006562599 26.938563771442105 24.836633470050185 92-93 23.66981015585396 26.53818452151384 27.47608894458692 22.315916378045287 94-95 22.784798073828533 26.087708596105124 27.657547229097222 23.469946100969118 96-97 20.736568331015185 26.460132385251622 29.43464247919855 23.368656804534645 98-99 21.89306351498995 27.541652153675038 26.625849307678706 23.93943502365631 100-101 21.40438360566921 26.061198481105443 27.50743203353331 25.02698587969204 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 51.0 1 39.0 2 38.0 3 63.0 4 70.0 5 72.0 6 120.5 7 234.0 8 416.0 9 539.5 10 521.5 11 527.0 12 600.0 13 678.5 14 776.0 15 899.0 16 994.0 17 1093.5 18 1261.0 19 1447.0 20 1639.5 21 1921.0 22 2297.5 23 2865.0 24 3611.0 25 4598.0 26 6130.0 27 8187.0 28 10185.0 29 12643.0 30 15801.0 31 19185.0 32 22910.5 33 25896.0 34 28047.0 35 30460.0 36 34155.0 37 37589.5 38 38881.5 39 39953.0 40 41853.5 41 44016.5 42 45480.0 43 46780.5 44 49475.5 45 53123.5 46 56976.5 47 76720.5 48 114564.0 49 111572.0 50 74254.5 51 55703.5 52 50602.5 53 49262.0 54 47736.0 55 44320.0 56 40065.0 57 36456.0 58 33987.5 59 30079.5 60 24981.5 61 21063.5 62 17446.5 63 13673.5 64 9789.0 65 6937.5 66 5010.0 67 3778.5 68 2908.0 69 2158.5 70 1703.0 71 1325.0 72 1023.5 73 912.5 74 717.0 75 524.0 76 404.5 77 258.5 78 169.0 79 112.5 80 72.5 81 55.0 82 35.5 83 24.0 84 19.5 85 16.0 86 11.5 87 5.0 88 3.5 89 4.5 90 4.5 91 4.0 92 2.5 93 1.0 94 1.0 95 1.5 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012820838305110563 2 0.011932265353271219 3 0.01637513011246795 4 0.019675543933585518 5 0.03198862626621646 6 0.05166417019980198 7 0.006156541166315469 8 0.025641676610221126 9 0.035415979080453935 10-11 0.04036659981213029 12-13 0.031100053314377114 14-15 0.028116986976059303 16-17 0.031004849069537183 18-19 0.024435756175582014 20-21 0.0551549925105994 22-23 0.012884307801670518 24-25 0.002951331590037828 26-27 0.0013963289243189723 28-29 1.2693899311990657E-4 30-31 0.0030782705831577345 32-33 0.0019992891416385286 34-35 0.0 36-37 3.490822310797431E-4 38-39 6.346949655995328E-5 40-41 0.0012376551829190892 42-43 0.0038716392901571505 44-45 0.0018088806519586688 46-47 6.981644621594862E-4 48-49 6.664297138795095E-4 50-51 0.0033638833176775247 52-53 0.0034908223107974305 54-55 0.0052997029627560996 56-57 6.981644621594862E-4 58-59 0.005839193683515702 60-61 0.002633984107238061 62-63 4.7602122419964965E-4 64-65 7.616339587194394E-4 66-67 0.01751758105054711 68-69 0.04284191017796847 70-71 0.05496458402091955 72-73 0.015930843636548273 74-75 0.03325801619741552 76-77 0.027323618269059893 78-79 0.05851887582827693 80-81 0.08416055243849806 82-83 0.050489984513442845 84-85 0.019675543933585518 86-87 0.020278504150905076 88-89 0.0232298357409429 90-91 0.041286907512249615 92-93 0.02535606387570134 94-95 0.013138185787910331 96-97 0.019612074437025565 98-99 0.05214019142400163 100-101 0.07143491837822742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1575560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.39634344284234 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1603116837991 46.0090877988862 2 11.849107984692955 13.601909427615247 3 3.5667896017026157 6.141620429030464 4 1.5420513405065992 3.540324333848487 5 0.8063551635826562 2.3140918952949727 6 0.45819940331357933 1.5779382190734978 7 0.31019024473122525 1.2462650073448904 8 0.2226312604373076 1.0222576228138067 9 0.16031060721462034 0.8281118402298855 >10 0.8443050244005638 8.769037753229343 >50 0.05163141162762008 2.030750081847538 >100 0.023485482208287507 2.454432992407157 >500 0.001323083366524071 0.45880505665568766 >1k 0.0022051389442067845 3.1518958140404387 >5k 6.615416832620355E-4 2.595760801917655 >10k+ 4.41027788841357E-4 4.2577109257647825 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24308 1.5428165223793444 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17754 1.1268374419254106 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13674 0.8678818959608012 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11544 0.7326918682881007 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8455 0.536634593414405 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7920 0.50267841275483 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6796 0.4313386986214425 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6391 0.4056335525146615 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6188 0.392749244712991 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5268 0.3343573078778339 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4944 0.31379319099240904 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3886 0.24664246363197848 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3683 0.23375815583030796 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3630 0.23039427251263042 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3475 0.2205565005458377 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2953 0.18742542334154205 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2909 0.1846327654929041 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2849 0.18082459569930692 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2845 0.1805707177130671 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2715 0.17231968316027318 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.14763004899845136 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2173 0.1379192160247785 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2073 0.13157226636878316 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1853 0.11760897712559346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.346949655995328E-5 7 0.0 0.0 0.0 0.0 6.346949655995328E-5 8 0.0 0.0 0.0 0.0 1.2693899311990657E-4 9 0.0 0.0 0.0 0.0 1.2693899311990657E-4 10-11 0.0 0.0 0.0 0.0 1.2693899311990657E-4 12-13 0.0 0.0 0.0 0.0 1.2693899311990657E-4 14-15 1.2693899311990657E-4 0.0 0.0 0.0 1.9040848967985985E-4 16-17 1.9040848967985985E-4 0.0 0.0 0.0 2.5387798623981313E-4 18-19 2.221432379598365E-4 0.0 0.0 3.173474827997664E-5 2.5387798623981313E-4 20-21 2.5387798623981313E-4 0.0 0.0 9.520424483992992E-5 2.5387798623981313E-4 22-23 2.5387798623981313E-4 0.0 0.0 1.2693899311990657E-4 3.808169793597197E-4 24-25 5.077559724796263E-4 0.0 0.0 2.856127345197898E-4 5.712254690395796E-4 26-27 5.077559724796263E-4 0.0 0.0 4.44286475919673E-4 6.346949655995328E-4 28-29 6.346949655995328E-4 0.0 0.0 0.0010472466932392292 6.664297138795095E-4 30-31 6.981644621594862E-4 0.0 0.0 0.0022849018761583184 7.298992104394627E-4 32-33 6.981644621594862E-4 0.0 0.0 0.004474599507476707 7.616339587194394E-4 34-35 8.568382035593694E-4 0.0 0.0 0.007743278580314301 7.616339587194394E-4 36-37 0.0010155119449592525 0.0 0.0 0.011392774632511614 7.616339587194394E-4 38-39 0.0011424509380791592 6.346949655995328E-5 0.0 0.01723196831602732 7.616339587194394E-4 40-41 0.0011424509380791592 6.346949655995328E-5 0.0 0.02373759171342253 7.616339587194394E-4 42-43 0.0013963289243189723 6.346949655995328E-5 0.0 0.03062403209017746 7.616339587194394E-4 44-45 0.0017136764071187388 6.346949655995328E-5 0.0 0.03674883850821295 7.616339587194394E-4 46-47 0.0020627586381984815 6.346949655995328E-5 0.0 0.04480946457132702 7.616339587194394E-4 48-49 0.0021579628830384117 6.346949655995328E-5 0.0 0.05099774048592247 7.616339587194394E-4 50-51 0.0021579628830384117 6.346949655995328E-5 0.0 0.05721775114879789 7.616339587194394E-4 52-53 0.0021579628830384117 6.346949655995328E-5 0.0 0.07000685470562848 7.933687069994161E-4 54-55 0.0021579628830384117 6.346949655995328E-5 0.0 0.09247505648785194 8.568382035593694E-4 56-57 0.0021579628830384117 6.346949655995328E-5 0.0 0.1282718525476656 8.88572951839346E-4 58-59 0.0021579628830384117 9.520424483992992E-5 0.0 0.17178019243951356 9.203077001193226E-4 60-61 0.0021579628830384117 1.2693899311990657E-4 0.0 0.2225875244357562 9.520424483992993E-4 62-63 0.0021579628830384117 1.2693899311990657E-4 0.0 0.25378278199497323 9.520424483992993E-4 64-65 0.0021579628830384117 1.2693899311990657E-4 0.0 0.29043641625834626 9.520424483992993E-4 66-67 0.0021579628830384117 1.2693899311990657E-4 0.0 0.37399400847952474 9.520424483992993E-4 68-69 0.0021579628830384117 1.2693899311990657E-4 0.0 0.4914125771154383 9.520424483992993E-4 70-71 0.002221432379598365 1.2693899311990657E-4 0.0 0.6089263499961919 0.0010155119449592525 72-73 0.002316636624438295 1.2693899311990657E-4 0.0 0.7397687171545355 0.0012693899311990657 74-75 0.0023483713727182717 1.2693899311990657E-4 0.0 0.8679453654573612 0.0012693899311990657 76-77 0.0023483713727182717 1.2693899311990657E-4 0.0 0.9776841250095205 0.0012693899311990657 78-79 0.0023483713727182717 1.2693899311990657E-4 0.0 1.0801873619538451 0.0012693899311990657 80-81 0.002570514610678108 1.2693899311990657E-4 0.0 1.1519396278148721 0.0012693899311990657 82-83 0.0026022493589580847 1.2693899311990657E-4 0.0 1.256981644621595 0.0013645941760389957 84-85 0.002633984107238061 1.2693899311990657E-4 0.0 1.5031480870293736 0.0013963289243189723 86-87 0.002665718855518038 1.2693899311990657E-4 0.0 1.8564827743786336 0.0013963289243189723 88-89 0.002665718855518038 1.2693899311990657E-4 0.0 2.2690979715148902 0.0013963289243189723 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5145 0.0 25.302528 1 GTATAGA 365 0.0 24.732014 1 TACATGG 5140 0.0 24.588444 2 GTAAGCG 280 0.0 23.75386 94-95 ACATGGG 5140 0.0 23.387497 3 GAGTACT 3965 0.0 20.790352 12-13 GTATAGC 655 0.0 19.584892 1 GTGTAGG 570 0.0 19.171305 1 CATGGGA 2365 0.0 19.087381 4 TAGCCCT 975 0.0 19.007013 4 GAGTACG 350 6.366463E-11 19.0046 1 GTGTAAG 655 0.0 18.859526 1 GTACTTT 4310 0.0 18.796345 14-15 AGTACTT 4335 0.0 18.24865 12-13 AGAGTAC 6990 0.0 18.21556 10-11 TACGCAG 265 3.9928273E-7 17.931145 4 TTTGCGT 375 1.70985E-10 17.73875 3 GTGTAGC 900 0.0 16.892977 1 CTTTGCG 370 2.73576E-9 16.69376 2 GTATATA 975 0.0 16.080816 1 >>END_MODULE