##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936567_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1575560 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13047551346823 31.0 31.0 34.0 31.0 34.0 2 32.24672306989261 33.0 31.0 34.0 31.0 34.0 3 32.38593960242707 34.0 31.0 34.0 31.0 34.0 4 36.03514623372008 37.0 35.0 37.0 35.0 37.0 5 35.93016324354515 37.0 35.0 37.0 35.0 37.0 6 36.00954708167255 37.0 35.0 37.0 35.0 37.0 7 35.95691500165021 37.0 35.0 37.0 35.0 37.0 8 35.93206605905202 37.0 35.0 37.0 35.0 37.0 9 37.583535377897384 39.0 37.0 39.0 35.0 39.0 10-11 37.475751161491786 39.0 37.0 39.0 35.0 39.0 12-13 37.38777799639493 39.0 37.0 39.0 34.0 39.0 14-15 38.482852128766915 40.0 38.0 41.0 34.0 41.0 16-17 38.51042486480998 40.0 38.0 41.0 34.0 41.0 18-19 38.671359072329835 40.0 38.0 41.0 34.0 41.0 20-21 38.6702712051588 40.0 38.0 41.0 34.0 41.0 22-23 38.57529005559928 40.0 38.0 41.0 34.0 41.0 24-25 38.43560480083272 40.0 38.0 41.0 34.0 41.0 26-27 38.23221140419914 40.0 38.0 41.0 34.0 41.0 28-29 38.30384371271167 40.0 38.0 41.0 34.0 41.0 30-31 38.25962451445835 40.0 38.0 41.0 34.0 41.0 32-33 38.09017999949225 40.0 37.0 41.0 33.5 41.0 34-35 37.87125752113534 40.0 37.0 41.0 33.0 41.0 36-37 37.693673360582906 40.0 37.0 41.0 33.0 41.0 38-39 37.495711683464926 40.0 37.0 41.0 33.0 41.0 40-41 37.372700817487114 40.0 37.0 41.0 32.5 41.0 42-43 37.122361573028 39.0 36.0 41.0 31.5 41.0 44-45 36.85875371296555 39.0 36.0 41.0 31.0 41.0 46-47 36.531670326740965 39.0 36.0 40.0 30.5 41.0 48-49 36.14149477011348 39.0 35.0 40.0 30.0 41.0 50-51 35.844819302343296 39.0 35.0 40.0 29.0 41.0 52-53 35.661149369113204 38.0 35.0 40.0 28.5 41.0 54-55 35.3751218614334 38.0 34.5 40.0 28.0 41.0 56-57 34.96326925029831 38.0 34.0 40.0 26.5 41.0 58-59 34.601743189723024 38.0 34.0 40.0 26.0 41.0 60-61 34.11599336109066 37.0 33.0 39.5 26.0 41.0 62-63 33.859671481885805 37.0 33.0 39.0 26.0 40.0 64-65 33.56961175708955 36.0 32.5 39.0 25.0 40.0 66-67 33.07440465612227 36.0 32.0 39.0 23.5 40.0 68-69 32.567351290969555 35.0 31.5 38.0 22.5 40.0 70-71 31.998577331234607 35.0 31.0 37.5 21.5 39.5 72-73 31.397277793292545 34.5 30.5 37.0 19.5 39.0 74-75 30.896880156896597 34.0 30.0 36.0 18.0 39.0 76-77 29.549649965726474 32.5 28.5 35.0 18.0 37.0 78-79 30.340599850211987 34.0 30.0 35.0 18.0 37.0 80-81 30.107334217674985 34.0 30.0 35.0 16.5 37.0 82-83 29.67931878189342 34.0 29.0 35.0 11.5 36.5 84-85 29.229378125872707 34.0 29.0 35.0 7.0 36.0 86-87 28.91487724999365 33.0 29.0 35.0 4.5 35.5 88-89 28.41294999873061 33.0 28.5 35.0 2.0 35.0 90-91 27.783725786387063 33.0 27.0 34.5 2.0 35.0 92-93 27.21892343039935 32.0 26.0 34.0 2.0 35.0 94-95 26.449508111401663 32.0 25.0 34.0 2.0 35.0 96-97 25.5824465586839 32.0 23.5 34.0 2.0 35.0 98-99 24.486925601056132 31.0 10.5 34.0 2.0 35.0 100-101 20.601865051156416 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17552415538344945 1101 2 0.3598244427834132 1101 3 0.19791957519365866 1101 4 0.13457988000976684 1101 5 0.18535633627208625 1101 6 0.14395005630359492 1101 7 0.16070399165676008 1101 8 0.1318139823490725 1101 9 0.2117610415450173 1101 10-11 0.1934539191487019 1101 12-13 0.23718454457652172 1101 14-15 0.26221488501503387 1101 16-17 0.2521621654333117 1101 18-19 0.18864344789302123 1101 20-21 0.0511428515008987 1101 22-23 0.1215244716383026 1101 24-25 0.26355832449431915 1101 26-27 0.18480164952830336 1101 28-29 0.12086659847398096 1101 30-31 0.24722976592084223 1101 32-33 0.30022908966115835 1101 34-35 0.3694305515873637 1101 36-37 0.315379219719226 1101 38-39 0.2113059802638091 1101 40-41 0.28563714763576087 1101 42-43 0.3169545491123458 1101 44-45 0.37345426156969097 1101 46-47 0.34536573204950116 1101 48-49 0.4142944491808933 1101 50-51 0.46535924268830087 1101 52-53 0.36713823140349433 1101 54-55 0.33806419506740326 1101 56-57 0.410362843390061 1101 58-59 0.42827556056186467 1101 60-61 0.37377522138276476 1101 62-63 0.25460263355981283 1101 64-65 0.3083777752894079 1101 66-67 0.32345210398292323 1101 68-69 0.3629419471671582 1101 70-71 0.12232546482025342 1101 72-73 0.23358500173935326 1101 74-75 0.3132520768414615 1101 76-77 0.31279650397247494 1101 78-79 0.4347821129079108 1101 80-81 0.551547389568551 1101 82-83 0.22043509643826908 1101 84-85 0.27262588095954143 1101 86-87 0.42062208483229924 1101 88-89 0.14066762548591072 1101 90-91 0.21943833097594379 1101 92-93 0.028720355049827617 1101 94-95 -0.1228874411031633 1101 96-97 0.1681389030624274 1101 98-99 -0.41952193296438267 1101 100-101 -0.01574783320370088 1102 1 0.1842925522570198 1102 2 0.28128347769243334 1102 3 0.17532710578582567 1102 4 0.11337064069103775 1102 5 0.026298301238341537 1102 6 0.012130480782872155 1102 7 0.1635211211901506 1102 8 0.026164479168592436 1102 9 0.09458794116945768 1102 10-11 0.17174902959923344 1102 12-13 0.20802822831659284 1102 14-15 0.18720215271553542 1102 16-17 0.33628238952179146 1102 18-19 0.18859277853784562 1102 20-21 0.09665475923243605 1102 22-23 0.06013465020230768 1102 24-25 0.13311050267029856 1102 26-27 0.19437382694206207 1102 28-29 0.1232092982758104 1102 30-31 0.08031105169254005 1102 32-33 0.09343575826797945 1102 34-35 0.17753249486540312 1102 36-37 0.15365281518202778 1102 38-39 0.16056365255693805 1102 40-41 0.25350866227704216 1102 42-43 0.11709534062830329 1102 44-45 0.08911126554701809 1102 46-47 -0.1519070007350436 1102 48-49 -0.053209387831813615 1102 50-51 -0.010489230821583817 1102 52-53 0.03568334531859563 1102 54-55 -0.026664000653155995 1102 56-57 -0.06561057122210912 1102 58-59 -0.11896906588805933 1102 60-61 -0.137270698045171 1102 62-63 -0.22291229874246454 1102 64-65 -0.22406511991423628 1102 66-67 -0.21573652806574728 1102 68-69 -0.2299685439011796 1102 70-71 -0.4134884582524023 1102 72-73 -0.47072531471458845 1102 74-75 -0.1918256854612288 1102 76-77 -0.3021088179554887 1102 78-79 -0.3103681368876785 1102 80-81 -0.18026209538827942 1102 82-83 -0.2684085509238052 1102 84-85 -0.3333165950309791 1102 86-87 -0.17254673333314585 1102 88-89 -0.3011340728469385 1102 90-91 -0.504124824694177 1102 92-93 -0.42384641590043515 1102 94-95 -0.34485472862100863 1102 96-97 -0.4101199160038007 1102 98-99 -0.5526814314330437 1102 100-101 -0.6166790233532318 1103 1 0.13026366015625968 1103 2 0.16560814880328678 1103 3 0.1980691269055228 1103 4 0.1028882207748083 1103 5 0.06220141904849896 1103 6 0.09747021000238476 1103 7 0.04386556989523882 1103 8 0.09739552748433056 1103 9 0.09177652886339871 1103 10-11 0.15938342432520614 1103 12-13 0.0451663396374542 1103 14-15 0.2213280892384475 1103 16-17 0.23170706948167208 1103 18-19 0.08542592997434184 1103 20-21 0.1600894386023839 1103 22-23 0.15781048791099295 1103 24-25 0.17643298364486526 1103 26-27 0.16740823494158974 1103 28-29 0.0755581641244305 1103 30-31 0.2475467605002848 1103 32-33 0.2828161783021983 1103 34-35 0.13322371514739473 1103 36-37 0.18709940387886803 1103 38-39 0.21733851097237533 1103 40-41 0.17966622672751953 1103 42-43 0.28040521172734856 1103 44-45 0.2426696839294138 1103 46-47 0.40589655805204927 1103 48-49 0.4371368675513452 1103 50-51 0.4557996311924555 1103 52-53 0.3995934759893558 1103 54-55 0.401204377318642 1103 56-57 0.272847521220811 1103 58-59 0.26755311569414175 1103 60-61 0.2354986076005119 1103 62-63 0.3495649887331993 1103 64-65 0.2988677085324625 1103 66-67 0.4574612183798905 1103 68-69 0.39154807016289084 1103 70-71 0.15433847673667955 1103 72-73 0.4250192210060142 1103 74-75 0.39080771374825574 1103 76-77 0.10299357275892262 1103 78-79 0.12631977975440734 1103 80-81 0.3719555097677585 1103 82-83 0.4970901659660534 1103 84-85 0.5486492550663584 1103 86-87 0.26632302361334936 1103 88-89 0.23039245951103737 1103 90-91 0.2947089461748007 1103 92-93 0.4766249795924544 1103 94-95 0.1833515718922385 1103 96-97 0.5136281298342027 1103 98-99 0.46416307626561704 1103 100-101 0.3186621807055232 1104 1 0.10716807055275979 1104 2 0.20562918963968713 1104 3 0.10527321074987839 1104 4 0.042885797704727224 1104 5 -0.003133802984464751 1104 6 -0.030663422823771214 1104 7 -0.07158656271314356 1104 8 0.026678431651397716 1104 9 0.06843837665370245 1104 10-11 0.09069667207747756 1104 12-13 0.017760726907440016 1104 14-15 -0.029292247374037572 1104 16-17 -0.09702660546705744 1104 18-19 -0.0555628819851961 1104 20-21 -0.015896249948248453 1104 22-23 -0.09314033705746994 1104 24-25 -0.1933898766411133 1104 26-27 -0.14142559357048157 1104 28-29 -0.07757999278826588 1104 30-31 -0.10831047466429311 1104 32-33 -0.14864020439156178 1104 34-35 -0.0505076185226514 1104 36-37 -0.08339739194322959 1104 38-39 0.04847966451730912 1104 40-41 0.02809718661088567 1104 42-43 -0.07495386272122317 1104 44-45 0.02052949450054342 1104 46-47 0.009346314620415797 1104 48-49 0.1834595775456478 1104 50-51 0.09596311685545089 1104 52-53 0.10920859124365734 1104 54-55 -0.049337388434885554 1104 56-57 0.27279806298656695 1104 58-59 0.24205528725568826 1104 60-61 0.0788542836166144 1104 62-63 -0.09607172149420506 1104 64-65 0.005779618177506052 1104 66-67 0.06732099214489295 1104 68-69 0.030545645048022152 1104 70-71 -0.030131663269738596 1104 72-73 0.13357820487062 1104 74-75 0.11933248836184518 1104 76-77 -0.002537135820379177 1104 78-79 0.0979961864130452 1104 80-81 0.08807623591176394 1104 82-83 0.025434973950531514 1104 84-85 0.07388880161486355 1104 86-87 0.1527419969677588 1104 88-89 -0.07631364253600026 1104 90-91 0.09961998269670502 1104 92-93 0.08873082174732971 1104 94-95 0.11094970111855318 1104 96-97 0.05244274759234102 1104 98-99 0.12868467839546582 1104 100-101 0.11815696548169186 1105 1 0.042204338263694297 1105 2 0.1782267675721485 1105 3 9.222314234591522E-4 1105 4 0.0773049249658655 1105 5 0.09056360446902545 1105 6 0.09625692136476971 1105 7 0.06251310746132788 1105 8 0.12262442626853698 1105 9 0.15835730636877798 1105 10-11 0.060959031803399455 1105 12-13 0.0760624637848224 1105 14-15 0.14884764361546132 1105 16-17 0.20033042466253903 1105 18-19 0.19523480574358842 1105 20-21 0.06227169039938474 1105 22-23 0.015253242368054032 1105 24-25 -0.01310167221477343 1105 26-27 0.10953103142597342 1105 28-29 0.12734703522397695 1105 30-31 0.07415905849397575 1105 32-33 0.1743248255896006 1105 34-35 -0.13034760621148678 1105 36-37 0.06288533139841235 1105 38-39 0.0739526017980765 1105 40-41 0.12809833259633052 1105 42-43 0.1788996497787494 1105 44-45 -0.17205718019894078 1105 46-47 0.010375927975836419 1105 48-49 0.03055592327090295 1105 50-51 0.1674934821956029 1105 52-53 0.1397683396507361 1105 54-55 0.10904885122668162 1105 56-57 0.25542369573425106 1105 58-59 0.25129984273338124 1105 60-61 0.20678827922039744 1105 62-63 0.26159947781776793 1105 64-65 0.15005222847042177 1105 66-67 0.2552943932769054 1105 68-69 0.14065165415915715 1105 70-71 -0.07496056764216164 1105 72-73 -0.05532112521902732 1105 74-75 0.1753947786737946 1105 76-77 0.14274858018041314 1105 78-79 0.04599777878711109 1105 80-81 0.3035579263385273 1105 82-83 0.22714178285036368 1105 84-85 0.41448809197701664 1105 86-87 0.4076200750301062 1105 88-89 0.34333149488402626 1105 90-91 0.562454856016199 1105 92-93 0.30286516071018 1105 94-95 0.2532386651695333 1105 96-97 0.08728329735976104 1105 98-99 0.0158977454672673 1105 100-101 0.24536557958145977 1106 1 0.13040512626619005 1106 2 0.2551692221766402 1106 3 0.192327480979138 1106 4 0.10569118847327985 1106 5 0.12602565694906076 1106 6 0.07534969662884095 1106 7 0.14193520878406218 1106 8 0.1272121607484351 1106 9 0.11730343711096225 1106 10-11 0.058631533465550945 1106 12-13 0.11622640245204252 1106 14-15 0.18412310817733868 1106 16-17 0.20133547708824295 1106 18-19 0.22320407597463543 1106 20-21 0.09415623484029823 1106 22-23 0.19070635673707415 1106 24-25 0.2526320225235139 1106 26-27 0.31195660968683114 1106 28-29 0.3795359669709555 1106 30-31 0.29502514981017214 1106 32-33 0.23346980019812236 1106 34-35 0.3079900991645701 1106 36-37 0.4256638227591054 1106 38-39 0.41170567580977746 1106 40-41 0.3468844698577769 1106 42-43 0.3940833790535905 1106 44-45 0.23625539239896653 1106 46-47 0.25435515301185774 1106 48-49 0.34960024388391986 1106 50-51 0.405542962595149 1106 52-53 0.27990087851405576 1106 54-55 0.34704012395678063 1106 56-57 0.3527784481964318 1106 58-59 0.3471617301623411 1106 60-61 0.22558870969844946 1106 62-63 0.25584581138134155 1106 64-65 0.3436562727392882 1106 66-67 0.16219009418779962 1106 68-69 0.2128715265162029 1106 70-71 0.3825289161722232 1106 72-73 0.5196910983915508 1106 74-75 0.30918979583858786 1106 76-77 0.34337796677506205 1106 78-79 0.26366519697946345 1106 80-81 0.3086438214560694 1106 82-83 0.2666185476422278 1106 84-85 0.6475449712180925 1106 86-87 0.7879541611826291 1106 88-89 0.808874494829702 1106 90-91 0.901930715355423 1106 92-93 0.6839900627272684 1106 94-95 0.7530118169466817 1106 96-97 0.8668481044854168 1106 98-99 1.2177960592557575 1106 100-101 0.9597669007662084 1107 1 0.03494853751832494 1107 2 0.12777448157712 1107 3 0.06759373570946536 1107 4 0.055098681546915884 1107 5 -0.006704585607948843 1107 6 0.10850897766712109 1107 7 0.06147616061482353 1107 8 0.02708175846597527 1107 9 0.03462671024841768 1107 10-11 0.13006740829835195 1107 12-13 0.12102656487975594 1107 14-15 0.31564153338810996 1107 16-17 0.26782840794071205 1107 18-19 0.15385478372927963 1107 20-21 0.02742795178664892 1107 22-23 0.10533065684271747 1107 24-25 0.1354724551917812 1107 26-27 0.07645176734724402 1107 28-29 0.11823440539358643 1107 30-31 0.1902006670463834 1107 32-33 0.2243905732342526 1107 34-35 0.11532209621837808 1107 36-37 0.33147985219766696 1107 38-39 0.3442132740889505 1107 40-41 0.26153652289948326 1107 42-43 0.3208784371320874 1107 44-45 0.21935020263644134 1107 46-47 0.239049408774882 1107 48-49 0.1701963185232529 1107 50-51 0.171786555227321 1107 52-53 0.21817122789796173 1107 54-55 0.1676237828089242 1107 56-57 0.2710641274258023 1107 58-59 0.11207975492745703 1107 60-61 0.09270527823779418 1107 62-63 -0.08510594634720547 1107 64-65 -0.05730682717602775 1107 66-67 -0.09895426161361343 1107 68-69 -0.0941780113791566 1107 70-71 -0.20735882685311324 1107 72-73 -0.1115386597671737 1107 74-75 -0.05980885996986629 1107 76-77 -0.35796465454087567 1107 78-79 -0.0015498937525499912 1107 80-81 -0.017230423417579743 1107 82-83 0.06225341248725158 1107 84-85 -0.026618761610233577 1107 86-87 0.06978084482727454 1107 88-89 0.1876688415566221 1107 90-91 0.13044382624410744 1107 92-93 0.4334851236175794 1107 94-95 0.4956959056406802 1107 96-97 0.6912906995456112 1107 98-99 0.9280282016107897 1107 100-101 0.8970976671964017 1108 1 -0.03690676532618653 1108 2 0.18670789304881197 1108 3 -0.01266674847229865 1108 4 0.06339159208380352 1108 5 0.0499658924267834 1108 6 0.034141914257212136 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-1.9078273035812607 2316 84-85 -1.7980771717129471 2316 86-87 -1.8733404955301936 2316 88-89 -1.9769313783210265 2316 90-91 -2.36918354769713 2316 92-93 -2.4338784893716507 2316 94-95 -2.6340140725151215 2316 96-97 -2.2124471335501106 2316 98-99 -1.6538361990485804 2316 100-101 -1.182747852872545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 14.0 5 27.0 6 77.0 7 152.0 8 369.0 9 757.0 10 1322.0 11 2229.0 12 3334.0 13 4590.0 14 6088.0 15 8316.0 16 10511.0 17 13454.0 18 12314.0 19 9089.0 20 8678.0 21 9271.0 22 9816.0 23 10963.0 24 12654.0 25 15308.0 26 18774.0 27 22863.0 28 28729.0 29 36301.0 30 45773.0 31 57570.0 32 73273.0 33 96606.0 34 131404.0 35 183316.0 36 262158.0 37 315358.0 38 157977.0 39 6123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67553522041363 19.123398242581626 14.964399043023931 21.236667493980814 2 15.654036239639153 22.034947270601407 37.99347722041739 24.31753926934205 3 23.083983245624 23.35063400168458 29.29343579594394 24.271946956747485 4 13.134927057449596 16.40336262459895 38.78290507154622 31.678805246405233 5 13.281146361542353 38.43778027012566 34.222707997326545 14.058365371005447 6 31.41606793774912 37.39629084262104 16.280687501586737 14.906953718043109 7 28.515294014601878 32.97436896679321 20.219783428146727 18.290553590458195 8 25.78210139946469 32.52782712621966 19.32402472563218 22.36604674868346 9 24.650043952765852 19.313575216352334 21.016403743442787 35.01997708743903 10-11 25.631458021274977 25.651863464419 26.825763538043617 21.89091497626241 12-13 28.52154154713245 23.211207443702556 26.23784559140877 22.029405417756227 14-15 23.25805384909787 25.680730106483384 27.335643213072757 23.725572831345985 16-17 21.92237689999508 28.464056591648035 27.302582200225643 22.31098430813124 18-19 21.416473478014574 29.14757391548572 28.34038650424539 21.095566102254317 20-21 23.37658175023436 25.69986715741624 27.78456906132402 23.138982031025375 22-23 22.183428120068204 26.148222736820593 28.328534493729034 23.33981464938217 24-25 24.005714815233244 26.037381137047667 28.239368501492173 21.717535546226916 26-27 23.30928261017201 25.016391007889897 30.23799806925669 21.436328312681404 28-29 21.517467287017723 28.806655667399422 28.482802458428257 21.193074587154594 30-31 22.55144390755996 26.017818528248217 27.994809434921496 23.43592812927033 32-33 22.62205118733208 26.594042425708043 28.37556418346602 22.40834220349386 34-35 24.25256117399227 25.814875722526843 28.155332719366672 21.777230384114215 36-37 23.55200714928123 25.222351912959535 29.631942765129228 21.593698172630006 38-39 22.496449489123137 26.84116402804864 29.32025370086212 21.342132781966104 40-41 22.710345118670542 27.22347204278861 28.11855090207637 21.947631936464475 42-43 21.968217921197912 27.62328773209473 26.853425678274522 23.55506866843283 44-45 23.28363419402341 25.393753014954175 27.518502297712445 23.804110493309977 46-47 24.682462108710553 27.252849780395543 27.09947574195841 20.96521236893549 48-49 24.19005033226914 26.741001440785006 27.25838289337556 21.81056533357029 50-51 21.458026032846984 27.34356554037072 29.45073714034736 21.74767128643494 52-53 23.36321806907858 28.645901265809126 26.079914138110826 21.910966527001463 54-55 22.213343530781962 26.85220945601795 27.081271232563715 23.85317578063638 56-57 23.664012385587093 26.216444989921477 27.13774680957826 22.98179581491317 58-59 25.755891853054507 25.992416486461956 26.038813778027997 22.21287788245554 60-61 23.221176320497207 26.960907056075968 28.892212670844984 20.925703952581838 62-63 22.392377052327795 26.999886072145213 28.316530297034355 22.29120657849264 64-65 21.883753123593706 29.168827344051984 26.984168291009446 21.96325124134486 66-67 23.192328515330214 26.548714130756963 26.27985546625335 23.979101887659475 68-69 22.485074150837953 27.01644548832034 26.85922939997975 23.63925096086195 70-71 24.41821422360225 26.75478294427018 26.51590548742172 22.311097344705853 72-73 23.79639890779056 25.547564553208396 28.626766240014422 22.02927029898662 74-75 22.21931652500335 29.198628454491388 26.720636466175673 21.861418554329592 76-77 22.987117795074784 26.66524070552072 26.435542483539827 23.912099015864662 78-79 23.207581301909606 27.216423192030238 26.78292937595806 22.793066130102098 80-81 24.50290920377248 26.616723272424224 26.530846699845196 22.349520823958105 82-83 23.64095258436159 26.341926133170258 27.85164559484794 22.165475687620216 84-85 22.822455342181964 27.710886961643528 27.449610799118524 22.017046897055987 86-87 22.72839354550639 27.9957805304612 26.48837376832967 22.78745215570274 88-89 22.221263104779528 28.243043180913 25.347876656791634 24.18781705751584 90-91 23.31360559891891 26.11357284630965 26.13064736547786 24.442174189293578 92-93 24.383804744482116 27.303066163853494 26.51012941314239 21.802999678522 94-95 23.730605234730135 26.895936286537943 26.76039666047625 22.613061818255673 96-97 21.408485567806952 27.173111739812732 28.84439317511421 22.57400951726611 98-99 22.717871984589532 28.503937507636213 25.627613170617924 23.15057733715633 100-101 21.781119012498635 27.516800674311913 26.145517831437253 24.556562481752195 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 99.0 1 79.0 2 68.0 3 77.0 4 73.0 5 82.0 6 135.0 7 283.5 8 470.0 9 622.0 10 681.5 11 660.5 12 722.0 13 801.5 14 904.5 15 1091.5 16 1245.0 17 1353.0 18 1493.5 19 1735.5 20 1984.5 21 2275.5 22 2690.5 23 3179.0 24 3908.5 25 4874.0 26 6189.5 27 8219.5 28 10523.0 29 13112.0 30 16326.0 31 19618.0 32 23073.5 33 26045.5 34 28309.5 35 30646.5 36 34109.5 37 37313.5 38 38528.5 39 39721.5 40 41661.5 41 43846.5 42 45341.0 43 46483.0 44 48369.0 45 50828.0 46 54303.0 47 77675.5 48 121050.5 49 114096.0 50 70654.0 51 52997.5 52 49050.5 53 48232.0 54 47379.5 55 44242.0 56 39829.0 57 36318.0 58 34487.5 59 31051.5 60 25359.5 61 20692.5 62 16901.0 63 13406.5 64 9625.0 65 6612.0 66 4949.5 67 3873.0 68 2964.5 69 2185.0 70 1668.0 71 1287.5 72 1041.5 73 962.5 74 793.0 75 646.5 76 508.0 77 310.5 78 198.5 79 136.5 80 89.0 81 62.5 82 48.0 83 33.5 84 17.0 85 7.0 86 4.5 87 1.5 88 0.5 89 2.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011487978877351545 2 0.01002818045647262 3 0.005775724186955749 4 3.173474827997664E-4 5 0.004506334255756683 6 0.0 7 5.712254690395796E-4 8 0.0018406154002386453 9 3.173474827997664E-4 10-11 0.0 12-13 0.0 14-15 5.712254690395796E-4 16-17 0.00396684353499708 18-19 0.0017454111553987153 20-21 6.981644621594862E-4 22-23 3.490822310797431E-4 24-25 3.808169793597197E-4 26-27 6.346949655995328E-5 28-29 3.173474827997664E-5 30-31 5.712254690395796E-4 32-33 0.0033638833176775247 34-35 8.251034552793927E-4 36-37 0.0010789814415192059 38-39 0.005236233466196147 40-41 3.173474827997664E-5 42-43 0.0011424509380791592 44-45 0.005077559724796263 46-47 0.0 48-49 0.0019040848967985986 50-51 7.616339587194394E-4 52-53 4.1255172763969637E-4 54-55 1.586737413998832E-4 56-57 0.011456244129071569 58-59 0.0023483713727182717 60-61 0.0028878620934778745 62-63 9.520424483992992E-5 64-65 0.0032369443245576176 66-67 6.981644621594862E-4 68-69 0.0013645941760389957 70-71 0.004188986772956917 72-73 0.0024118408692782247 74-75 0.007045114118154815 76-77 9.203077001193226E-4 78-79 0.006664297138795096 80-81 0.0027291883520779913 82-83 0.0022531671278783415 84-85 0.0015232679174388788 86-87 4.1255172763969637E-4 88-89 0.002380106120998248 90-91 0.0071720531112747215 92-93 0.0015550026657188552 94-95 3.808169793597197E-4 96-97 1.586737413998832E-4 98-99 4.7602122419964965E-4 100-101 0.0017771459036786921 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1575560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.332396962207675 #Duplication Level Percentage of deduplicated Percentage of total 1 78.26076549239723 42.5209497729917 2 12.75778971249972 13.863225900398083 3 3.959355606769862 6.453638416246883 4 1.7582961738148244 3.8212978277135186 5 0.9077077715137429 2.465896948378279 6 0.5387525909900829 1.7563031782854657 7 0.36056928014303 1.3713415282776 8 0.23491964111433433 1.0210997756194702 9 0.18333185814444738 0.8964773363270935 >10 0.9437838181245338 9.254446078776379 >50 0.0587788443295331 2.1969609160333707 >100 0.03048132959958391 2.9969207767566135 >500 0.0018614061477815848 0.6754530953086466 >1k 0.00244309556896333 3.2054629767258174 >5k 8.143651896544435E-4 3.6758023443275047 >10k+ 3.4901365270904714E-4 3.824723127833698 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35101 2.2278427987509204 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13349 0.8472543095788164 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12059 0.7653786590164767 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9756 0.6192084084389042 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9475 0.6013734799055575 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8609 0.5464088958846379 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8370 0.5312396862068091 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7904 0.5016629008098707 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7419 0.4708801949782934 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6620 0.42016806722689076 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4517 0.286691715961309 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4346 0.275838432049557 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3894 0.24715021960445813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3469 0.22017568356647796 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3086 0.19586686638401585 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2970 0.18850440478306124 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2720 0.17263703064307295 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2716 0.17238315265683313 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2649 0.16813069638731626 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2636 0.16730559293203687 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2424 0.15385005966132675 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2061 0.13081063241006372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1971 0.12509837771966792 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1881 0.11938612302927214 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1804 0.11449897179415572 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1626 0.10320140140648404 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1604 0.10180507248216508 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.346949655995328E-5 0.0 2 1.9040848967985985E-4 0.0 0.0 6.346949655995328E-5 0.0 3 1.9040848967985985E-4 0.0 0.0 6.346949655995328E-5 0.0 4 1.9040848967985985E-4 0.0 0.0 6.346949655995328E-5 0.0 5 1.9040848967985985E-4 0.0 0.0 6.346949655995328E-5 0.0 6 1.9040848967985985E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 7 2.5387798623981313E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 8 2.5387798623981313E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 9 2.5387798623981313E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 10-11 2.5387798623981313E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 12-13 2.5387798623981313E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 14-15 2.5387798623981313E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 16-17 3.173474827997664E-4 0.0 0.0 6.346949655995328E-5 6.346949655995328E-5 18-19 4.1255172763969637E-4 0.0 0.0 9.520424483992992E-5 6.346949655995328E-5 20-21 4.44286475919673E-4 0.0 0.0 1.586737413998832E-4 9.520424483992992E-5 22-23 4.44286475919673E-4 0.0 0.0 1.9040848967985985E-4 2.221432379598365E-4 24-25 4.44286475919673E-4 0.0 0.0 4.1255172763969637E-4 3.173474827997664E-4 26-27 4.44286475919673E-4 0.0 0.0 5.712254690395796E-4 3.808169793597197E-4 28-29 6.664297138795095E-4 0.0 0.0 0.0011741856863591359 3.808169793597197E-4 30-31 8.88572951839346E-4 0.0 0.0 0.0024118408692782247 3.808169793597197E-4 32-33 8.88572951839346E-4 0.0 0.0 0.004665007997156566 3.808169793597197E-4 34-35 0.0010155119449592525 0.0 0.0 0.007965421818274138 4.44286475919673E-4 36-37 0.0012693899311990657 0.0 0.0 0.011964000101551193 4.44286475919673E-4 38-39 0.0013963289243189723 0.0 0.0 0.01843788875066643 4.44286475919673E-4 40-41 0.0013963289243189723 0.0 0.0 0.02494351214806164 4.44286475919673E-4 42-43 0.0015550026657188557 0.0 0.0 0.03195689151793648 4.44286475919673E-4 44-45 0.001777145903678692 0.0 0.0 0.03785955469801214 4.44286475919673E-4 46-47 0.0024435756175582015 0.0 0.0 0.045824976516286274 4.44286475919673E-4 48-49 0.002665718855518038 0.0 0.0 0.051949782934321766 5.077559724796263E-4 50-51 0.002665718855518038 0.0 0.0 0.05804285460407728 5.077559724796263E-4 52-53 0.002697453603798015 0.0 0.0 0.07073675391606793 5.712254690395796E-4 54-55 0.002856127345197898 0.0 0.0 0.09326842519485136 6.346949655995328E-4 56-57 0.002856127345197898 0.0 0.0 0.129096956002945 6.346949655995328E-4 58-59 0.002856127345197898 0.0 0.0 0.17301784762243266 6.346949655995328E-4 60-61 0.002856127345197898 0.0 0.0 0.22382517961867526 6.981644621594862E-4 62-63 0.002856127345197898 0.0 0.0 0.2548617634364924 6.981644621594862E-4 64-65 0.002856127345197898 0.0 0.0 0.2914201934550255 6.981644621594862E-4 66-67 0.0029195968417578514 0.0 0.0 0.37548554164868364 6.981644621594862E-4 68-69 0.0029195968417578514 0.0 0.0 0.4935070705019168 6.981644621594862E-4 70-71 0.0030465358348777576 0.0 0.0 0.6120046205793496 6.981644621594862E-4 72-73 0.0031734748279976643 0.0 0.0 0.7431008657239331 7.616339587194394E-4 74-75 0.0032369443245576176 0.0 0.0 0.8718804742440782 7.616339587194394E-4 76-77 0.0032369443245576176 0.0 0.0 0.9817144380410775 7.933687069994161E-4 78-79 0.0032369443245576176 0.0 0.0 1.0837733885094822 8.251034552793927E-4 80-81 0.003459087562517454 0.0 0.0 1.1551448373911497 8.568382035593694E-4 82-83 0.0035225570590774074 0.0 0.0 1.2601233847013127 8.88572951839346E-4 84-85 0.003617761303917337 0.0 0.0 1.5064167661022112 8.88572951839346E-4 86-87 0.0036494960521973136 0.0 0.0 1.860037066185991 8.88572951839346E-4 88-89 0.0037129655487572674 0.0 0.0 2.273001345553327 9.837771966792759E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 320 0.0 28.943022 94-95 GTAAGGT 475 0.0 25.999594 4 GGACCGT 170 1.123899E-7 25.145864 6 TAAGGTG 590 0.0 23.34709 5 GAGTACT 5110 0.0 23.00673 12-13 GTACATG 5175 0.0 21.394285 1 AGTACTT 5435 0.0 20.800701 12-13 GTACTTT 5590 0.0 20.776272 14-15 TACATGG 5250 0.0 20.26892 2 ACATGGG 5240 0.0 19.399782 3 AGAGTAC 8455 0.0 19.298061 10-11 TACTTTT 6250 0.0 17.974287 14-15 GGTAAGG 665 0.0 17.857996 3 ATACCGT 160 0.00119166 17.811655 6 ACTTTTT 6710 0.0 17.732302 16-17 AGGTAAG 620 0.0 17.622929 2 CGTATAG 110 0.009919664 17.279037 1 GTATAGG 400 4.292815E-10 16.631073 1 GTGTAGC 775 0.0 16.554432 1 TATACTG 685 0.0 15.948655 5 >>END_MODULE