FastQCFastQC Report
Thu 26 May 2016
SRR936566_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936566_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777241
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT267231.5036227500941066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT182161.0249594737010905No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT139680.7859373039447097No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC118460.6665387530447474No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT87170.4904793441069613No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT84110.4732616454380695No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC72580.40838580698959787No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC67900.38205285608423395No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC67010.37704509405308567No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG56180.3161079448425959No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA52550.29568302779420463No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA41400.23294533493206604No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA39250.22084793227255053No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT39240.2207916652834365No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC36040.20278622876694832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33230.18697520482590713No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC31790.17887275839348743No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG31180.17544047205753188No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC28700.16148625875725353No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC27740.15608462780230706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27320.153721414259518No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT23240.13076448270099553No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG22160.12468764787668077No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG18630.10482540071942972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC600.003941651431.6828583
GTACATG59850.025.5722241
TACATGG62250.023.742392
ACATGGG61350.023.0068663
GAGTACT45150.021.52418512-13
GTACTTT46100.020.6141414-15
CGATCGT1452.4519977E-919.65028224-25
CATGGGT11600.019.2555314
TATACTG8400.019.2398135
ATGGGCG2803.463174E-818.6739375
GTGTAAG7700.018.5185641
CATGGGG30500.018.3864444
TAACGCT1300.0075424418.2785724
AGTACTT47500.018.2082812-13
AGAGTAC80300.018.19890210-11
GTATTAG5550.017.9846951
CTCGCGC1851.4370016E-1017.96799760-61
ATTCGCG1608.28004E-917.81383380-81
GTGTAGC10850.017.5229421
TAAGGTG7400.017.3433635
Produced by FastQC (version 0.11.5)