FastQCFastQC Report
Thu 26 May 2016
SRR936566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936566_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1777241
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT350861.9741835800547027No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT135300.7612923627127666No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT135070.7599982219631439No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC101700.5722352792896405No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT96350.5421324401136368No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC88630.49869432451760903No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT84740.4768064657522531No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC84060.47298031049249933No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG73020.41086155451061507No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA65800.37023678837028856No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC54020.3039542751939664No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA46700.26276683916249965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.2422856551249943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.21870978668621757No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT33510.18855068052109986No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC33270.18720027278236323No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA28820.16216146262662184No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG28190.15861664231243822No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC28090.15805397242129796No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG27350.15389021522686008No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT26850.15107686577115878No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC22810.12834500216909242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22170.12474391486579478No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC21090.11866708004148004No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG18090.10178698330727234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT60000.022.47680312-13
GTACTTT64500.020.7982214-15
TGAACCG2309.0525646E-820.6467555
TACATGG58300.020.5390592
GTACATG58900.020.4955351
TAGCCCT9650.019.6900484
AGTACTT64100.019.52045812-13
GTGTAGC10350.019.5168511
ACATGGG58500.019.4908393
ATACCGT2201.3489826E-619.426726
GTATAGC6050.019.079051
AGAGTAC98950.018.71613910-11
CGATACA1558.8548206E-418.6174581
ACGACGG1300.007576168518.2644376
ACTTTTT74950.018.15285116-17
TAAGGTG6350.017.9480445
GTAAGGT5850.017.8640884
TCGCGTA1350.00941658317.5879769
ACCGGTC1650.001466814117.2681968
TACTTTT71950.017.02771414-15
Produced by FastQC (version 0.11.5)