##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936566_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1777241 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.617735580036697 31.0 31.0 34.0 30.0 34.0 2 31.85580627500716 31.0 31.0 34.0 30.0 34.0 3 32.16230494344886 33.0 31.0 34.0 30.0 34.0 4 35.90805748910812 37.0 35.0 37.0 35.0 37.0 5 35.75982885832591 37.0 35.0 37.0 35.0 37.0 6 35.8813469867058 37.0 35.0 37.0 35.0 37.0 7 35.82979742195909 37.0 35.0 37.0 35.0 37.0 8 35.8132594285187 37.0 35.0 37.0 35.0 37.0 9 37.47570194475595 39.0 37.0 39.0 35.0 39.0 10-11 37.36126248494155 39.0 37.0 39.0 34.0 39.0 12-13 37.266292810035324 39.0 37.0 39.0 34.0 39.0 14-15 38.380203641487 40.0 38.0 41.0 34.0 41.0 16-17 38.335105368377164 40.0 38.0 41.0 33.5 41.0 18-19 38.40062208783164 40.0 38.0 41.0 33.5 41.0 20-21 38.472548742686 40.0 38.0 41.0 34.0 41.0 22-23 38.39185512825779 40.0 38.0 41.0 34.0 41.0 24-25 38.266906682886564 40.0 38.0 41.0 33.5 41.0 26-27 38.06035000317908 40.0 37.5 41.0 33.0 41.0 28-29 38.01958878959016 40.0 37.5 41.0 33.0 41.0 30-31 38.087277977494324 40.0 37.5 41.0 33.5 41.0 32-33 37.941644661585 40.0 37.0 41.0 33.0 41.0 34-35 37.749226188232214 40.0 37.0 41.0 33.0 41.0 36-37 37.52573089412184 40.0 37.0 41.0 32.5 41.0 38-39 37.372083752287956 39.0 37.0 41.0 32.0 41.0 40-41 37.223344217244595 39.0 36.0 41.0 31.5 41.0 42-43 37.00158335307367 39.0 36.0 41.0 31.0 41.0 44-45 36.758947154606496 39.0 36.0 41.0 31.0 41.0 46-47 36.42789666679983 39.0 35.5 40.0 30.0 41.0 48-49 35.969512294618454 39.0 35.0 40.0 29.0 41.0 50-51 35.79751114227052 39.0 35.0 40.0 28.5 41.0 52-53 35.59653445987348 38.0 35.0 40.0 28.0 41.0 54-55 35.36785641339582 38.0 34.5 40.0 28.0 41.0 56-57 35.028135182566686 38.0 34.0 40.0 27.0 41.0 58-59 34.6502393316382 38.0 34.0 40.0 26.0 41.0 60-61 34.17755779885789 37.0 33.0 40.0 26.0 41.0 62-63 33.95333666058796 37.0 33.0 39.0 26.0 41.0 64-65 33.55317652473694 36.0 32.5 39.0 25.0 40.0 66-67 33.09907941579111 36.0 32.0 39.0 23.5 40.0 68-69 32.59808658476819 35.0 31.5 38.0 22.5 40.0 70-71 32.06328517066622 35.0 31.0 37.5 21.5 39.5 72-73 31.616232407422515 34.5 31.0 37.0 20.5 39.0 74-75 31.265043682877 34.0 30.5 36.5 20.0 39.0 76-77 29.871034654275924 33.0 28.5 35.0 19.0 37.0 78-79 30.68332966660121 34.0 30.0 35.0 20.0 37.0 80-81 30.461239640543965 34.0 30.0 35.0 18.0 37.0 82-83 30.099054095646004 34.0 30.0 35.0 16.5 36.5 84-85 29.64740066203739 34.0 29.0 35.0 11.5 36.0 86-87 29.36177507721238 34.0 29.0 35.0 7.0 36.0 88-89 28.94607034161377 34.0 29.0 35.0 4.5 35.0 90-91 28.43685915416086 33.0 29.0 35.0 2.0 35.0 92-93 28.000555355182556 33.0 27.0 35.0 2.0 35.0 94-95 27.447615433134843 33.0 26.5 34.5 2.0 35.0 96-97 26.719272175242413 32.5 25.0 34.0 2.0 35.0 98-99 25.919129707225977 32.0 24.5 34.0 2.0 35.0 100-101 23.04970907153279 29.5 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2822045612847397 1101 2 0.24981278238912452 1101 3 0.08732309558819651 1101 4 0.005948363891789654 1101 5 0.12550233142977163 1101 6 0.04366586992122734 1101 7 0.0974120546259627 1101 8 0.011392056797227212 1101 9 0.10387528121705714 1101 10-11 0.1302796868432381 1101 12-13 0.08740935729109367 1101 14-15 0.19418150185590832 1101 16-17 0.15272688654182787 1101 18-19 0.11682050239692643 1101 20-21 0.04614423890065922 1101 22-23 0.0059291020733738264 1101 24-25 -0.0057465394194125 1101 26-27 0.0533540679275859 1101 28-29 0.021435594615020648 1101 30-31 -0.01924952988010631 1101 32-33 0.1492210791460522 1101 34-35 0.09830090834998373 1101 36-37 0.09058266416083427 1101 38-39 0.14161161376103593 1101 40-41 0.19276527195441417 1101 42-43 0.19499667956658584 1101 44-45 0.20022584313815628 1101 46-47 0.1896045993814255 1101 48-49 0.33411720181756266 1101 50-51 0.1325435843503726 1101 52-53 0.22322031699690825 1101 54-55 0.09782893776961998 1101 56-57 0.03847206170053141 1101 58-59 0.16492199914238626 1101 60-61 0.12576402092194172 1101 62-63 -0.04901129496246881 1101 64-65 -0.1247693904358016 1101 66-67 -0.07763827804448908 1101 68-69 -0.18885808276588278 1101 70-71 -0.23135122831338606 1101 72-73 -0.13979796163852498 1101 74-75 -0.10200098211910102 1101 76-77 0.29264085702685705 1101 78-79 0.21179203400063074 1101 80-81 -0.009286126274609785 1101 82-83 0.1625885094325774 1101 84-85 0.3151908510686603 1101 86-87 0.09714888807189226 1101 88-89 0.03772788091867341 1101 90-91 0.023197188019498327 1101 92-93 0.044856394578189906 1101 94-95 -0.19050788686048392 1101 96-97 -0.07288706923900534 1101 98-99 -0.10031983802317157 1101 100-101 -0.13453685598848608 1102 1 0.3426101703797251 1102 2 0.40085356535155015 1102 3 0.19237937366982294 1102 4 0.09047195786971685 1102 5 -0.007740624462925894 1102 6 0.11256547808320505 1102 7 0.05521383689490733 1102 8 -0.09718878963817446 1102 9 0.011911395753855913 1102 10-11 0.1432061091302117 1102 12-13 0.03640655107345481 1102 14-15 0.049335521184765696 1102 16-17 0.1331977108744269 1102 18-19 0.09123915074365385 1102 20-21 0.1134327296884905 1102 22-23 0.005560911744034058 1102 24-25 0.03753869383296404 1102 26-27 -0.21841243335709493 1102 28-29 -0.12365369533562642 1102 30-31 0.008999778244707102 1102 32-33 0.1044588143276215 1102 34-35 0.2527352104793792 1102 36-37 0.2738894460369039 1102 38-39 0.19257418474681032 1102 40-41 0.2639959614154819 1102 42-43 0.2586937014382258 1102 44-45 0.13320191797872383 1102 46-47 0.2813421616653713 1102 48-49 0.23202447630953316 1102 50-51 0.29808727051253214 1102 52-53 0.12303297644324829 1102 54-55 0.2772614653734564 1102 56-57 0.325954501489079 1102 58-59 0.32130818448940346 1102 60-61 0.5305286505091829 1102 62-63 0.37069398575988544 1102 64-65 0.2831301710381311 1102 66-67 0.4084097757279963 1102 68-69 0.37066263591127324 1102 70-71 0.2324675241135381 1102 72-73 0.1839358526956154 1102 74-75 -0.16595829937048734 1102 76-77 0.23232188653256713 1102 78-79 0.3436722112026658 1102 80-81 0.35758830222377824 1102 82-83 0.4146796027566815 1102 84-85 0.6672129751320988 1102 86-87 0.3609769021936984 1102 88-89 0.17113955908084932 1102 90-91 0.2121746703340257 1102 92-93 0.5367786973088364 1102 94-95 0.1556504266304266 1102 96-97 0.16553960793437383 1102 98-99 -0.30250624329748277 1102 100-101 -0.17114180712355775 1103 1 0.38221311059218976 1103 2 0.3855129335830405 1103 3 0.1507435368651855 1103 4 0.09492010862587108 1103 5 0.021018278230435783 1103 6 -0.05005932879161179 1103 7 0.05703258248909293 1103 8 0.10313159102003056 1103 9 0.027544995314947585 1103 10-11 0.037240677407375244 1103 12-13 0.1465482156111051 1103 14-15 0.057128003100636704 1103 16-17 0.04908029461198993 1103 18-19 0.07267923738830717 1103 20-21 0.1312422494885226 1103 22-23 0.06620601375121993 1103 24-25 -0.033561359858580886 1103 26-27 -0.031098938403196996 1103 28-29 0.07034182979280246 1103 30-31 0.2345854591170493 1103 32-33 0.006313354280713668 1103 34-35 0.0924952844672049 1103 36-37 0.1632794943977416 1103 38-39 0.21194869850965858 1103 40-41 0.23918030140267632 1103 42-43 0.14413464391792985 1103 44-45 0.26645545411343363 1103 46-47 0.14258077335944108 1103 48-49 0.31117460826998666 1103 50-51 0.04151044153517347 1103 52-53 0.15166173530749205 1103 54-55 0.19694224966592344 1103 56-57 0.2743361331774281 1103 58-59 0.007303804966930727 1103 60-61 -0.010472999453128295 1103 62-63 -0.11057606806814846 1103 64-65 -0.09510768536036807 1103 66-67 -0.11010534661969018 1103 68-69 -0.2024002443196835 1103 70-71 -0.06237543534560075 1103 72-73 -0.14260674095747916 1103 74-75 -0.06475820794300446 1103 76-77 -0.2534556228836351 1103 78-79 0.022112472974388453 1103 80-81 0.07361629333554731 1103 82-83 0.08150467195686417 1103 84-85 0.13366143461940183 1103 86-87 0.0574958142835591 1103 88-89 0.19907126990318602 1103 90-91 -0.14631996349179488 1103 92-93 0.04043954518146364 1103 94-95 0.08516687406144996 1103 96-97 -0.3470082180004681 1103 98-99 -0.3403877093744434 1103 100-101 -0.08788945324075925 1104 1 0.20702813090855798 1104 2 0.3175499832282149 1104 3 0.037329462496359156 1104 4 -0.05247304500445438 1104 5 -0.011007530575874114 1104 6 -0.017808303888664057 1104 7 -0.00584244074629936 1104 8 0.004663240170884819 1104 9 0.009004998593354685 1104 10-11 8.186623742076904E-4 1104 12-13 -0.022733127411569853 1104 14-15 -0.023049027133623667 1104 16-17 -0.06278122623956506 1104 18-19 -0.038761600559332976 1104 20-21 -0.08483981702089949 1104 22-23 0.026564586528074585 1104 24-25 -0.026531143565271975 1104 26-27 -0.0966184538371877 1104 28-29 -0.08055724763980976 1104 30-31 -0.154670315489561 1104 32-33 -0.17893606955141905 1104 34-35 -0.1445245914523099 1104 36-37 -0.16451923987980877 1104 38-39 -0.10942049556212652 1104 40-41 -0.11885299504020708 1104 42-43 -0.09036981977326519 1104 44-45 -0.09371420069297898 1104 46-47 -0.04464568042742911 1104 48-49 0.041470645644679394 1104 50-51 -0.07364706098603335 1104 52-53 -0.06991716130533376 1104 54-55 -0.05739460772696248 1104 56-57 -0.054340704285436914 1104 58-59 0.05153071577476709 1104 60-61 -0.01107926601115139 1104 62-63 -0.33233535718391494 1104 64-65 -0.32426975569526917 1104 66-67 -0.09056645261841112 1104 68-69 -0.2551258682211852 1104 70-71 -0.09411922488195756 1104 72-73 -0.11850232933752736 1104 74-75 -0.20360343237344125 1104 76-77 -0.023993863398853676 1104 78-79 0.07480465388281132 1104 80-81 0.16763852304278615 1104 82-83 0.13645570243031813 1104 84-85 0.12750612695684183 1104 86-87 0.2557871069460873 1104 88-89 0.13347947767222124 1104 90-91 0.1291391888371436 1104 92-93 0.22020272087218373 1104 94-95 0.23850778913761062 1104 96-97 -0.16184115377814123 1104 98-99 -0.25014324009677225 1104 100-101 -0.15488415407372358 1105 1 0.35966451457047555 1105 2 0.34883625547872654 1105 3 0.07795332402425714 1105 4 0.057922952657179394 1105 5 -0.1366247413086441 1105 6 -0.08000848858590359 1105 7 -0.03764849169900941 1105 8 -0.018293864318614794 1105 9 -0.07914938457716403 1105 10-11 -0.003002155885404534 1105 12-13 -0.04406396422562864 1105 14-15 0.038002836359865455 1105 16-17 -0.005527623372529433 1105 18-19 -0.10935460155059928 1105 20-21 -0.10650870061927975 1105 22-23 -0.11894210239263714 1105 24-25 -0.1751638121015091 1105 26-27 -0.11835543395257986 1105 28-29 -0.048059535174438395 1105 30-31 -0.03694255752766651 1105 32-33 -0.09278635040983119 1105 34-35 -0.09428175270655004 1105 36-37 0.050061145781583605 1105 38-39 -0.12121824361136646 1105 40-41 -0.21523392462649582 1105 42-43 -0.09587705939675573 1105 44-45 -0.22508869129290332 1105 46-47 -0.2149015877738023 1105 48-49 -0.18862949753804514 1105 50-51 -0.2099645326672004 1105 52-53 -0.4041669558508403 1105 54-55 -0.35590199138041356 1105 56-57 -0.3813407085216056 1105 58-59 -0.28087930786309556 1105 60-61 -0.17770434755752973 1105 62-63 -0.28913897233586994 1105 64-65 -0.2921888828737238 1105 66-67 -0.3146895643432899 1105 68-69 -0.5022217582839588 1105 70-71 -0.4331291036426208 1105 72-73 -0.5514127511758566 1105 74-75 -0.5207481867176398 1105 76-77 -0.4016339537141107 1105 78-79 -0.43679473526072243 1105 80-81 -0.5316476814289182 1105 82-83 -0.26195594366265595 1105 84-85 -0.3113710536143621 1105 86-87 -0.3290775684358067 1105 88-89 -0.21635340521447688 1105 90-91 -0.43482271523890503 1105 92-93 -0.3143009264747043 1105 94-95 -0.18726584236597432 1105 96-97 -0.21339222934303237 1105 98-99 -0.16006605698386167 1105 100-101 -0.11876292321064241 1106 1 0.20757637602924106 1106 2 0.324107552790327 1106 3 0.08907462322643767 1106 4 -0.01420432075757816 1106 5 -0.12837900455211582 1106 6 -0.07262201753058406 1106 7 -0.059581036032966495 1106 8 -0.06687026239779215 1106 9 -0.15472884538557707 1106 10-11 -0.14664510215530413 1106 12-13 -0.00957895647428586 1106 14-15 -0.011070309932719624 1106 16-17 0.047662719577530765 1106 18-19 -0.05560512145833485 1106 20-21 -0.12525562843718063 1106 22-23 -0.048642068362319435 1106 24-25 -0.020261082113229634 1106 26-27 -0.0799403963301657 1106 28-29 -0.10844114745560063 1106 30-31 -0.20694009978198835 1106 32-33 -0.2145435440423782 1106 34-35 -0.37541430082164595 1106 36-37 -0.22241162995857167 1106 38-39 -0.04152111350209253 1106 40-41 0.0541042800848075 1106 42-43 -0.12128447800755993 1106 44-45 -0.020180289583827005 1106 46-47 0.03814034514864062 1106 48-49 -0.06983908652734527 1106 50-51 -0.09588495842437794 1106 52-53 0.006498242470335924 1106 54-55 -0.05190248292954891 1106 56-57 -0.014405422855944039 1106 58-59 -7.506543296145196E-4 1106 60-61 0.1487069656348936 1106 62-63 -0.21910125539254466 1106 64-65 -0.027324720586882734 1106 66-67 -0.13094660672995673 1106 68-69 -0.12754031993556936 1106 70-71 0.051740635836331705 1106 72-73 0.1690545986180716 1106 74-75 0.12755360554613304 1106 76-77 0.030803090185010262 1106 78-79 0.10426332425453566 1106 80-81 0.057938093895316456 1106 82-83 0.2581446348528651 1106 84-85 0.4199136856755388 1106 86-87 0.47161863529240833 1106 88-89 0.2131801902783934 1106 90-91 0.018457902600548692 1106 92-93 0.3191593903854333 1106 94-95 0.4638680291506425 1106 96-97 0.34461056870589246 1106 98-99 0.15082270166551126 1106 100-101 0.16986716715877392 1107 1 0.48263073403704126 1107 2 0.5291238176145932 1107 3 0.27362786568870234 1107 4 0.11311831317885179 1107 5 0.02990072771695651 1107 6 0.07039773301867314 1107 7 0.06340714997077157 1107 8 0.08961409441782564 1107 9 0.08103020813222628 1107 10-11 0.18782979665081 1107 12-13 0.19597024390948548 1107 14-15 0.24363314797459168 1107 16-17 0.28170445744419226 1107 18-19 0.13475718956598115 1107 20-21 0.16801291999112067 1107 22-23 0.17438330249270706 1107 24-25 0.040199782959696506 1107 26-27 0.24818540200872263 1107 28-29 0.2304554843348896 1107 30-31 0.2534537757878539 1107 32-33 0.25490526859706364 1107 34-35 0.1758936976114711 1107 36-37 0.459570606468958 1107 38-39 0.33933550057592043 1107 40-41 0.3514418731178992 1107 42-43 0.09877391072458153 1107 44-45 -0.04425393262061306 1107 46-47 0.07765279781602175 1107 48-49 -0.025015728763662537 1107 50-51 -0.07925538199105375 1107 52-53 -0.23653172681668622 1107 54-55 0.015296362353083737 1107 56-57 0.06091235150297791 1107 58-59 -0.22436573150947225 1107 60-61 0.0035760522500467573 1107 62-63 -0.2573888787980039 1107 64-65 -0.12425299640197096 1107 66-67 -0.202576410246337 1107 68-69 -0.14899470178708896 1107 70-71 -0.2620242877786012 1107 72-73 -0.24545572514560732 1107 74-75 -0.0932754463253147 1107 76-77 -0.06542477631285948 1107 78-79 0.03647548228164155 1107 80-81 0.005075286126803746 1107 82-83 0.02655894222113986 1107 84-85 -0.10476527564653892 1107 86-87 0.11165082431634943 1107 88-89 0.0682756503202917 1107 90-91 0.17044814370598615 1107 92-93 0.2689477773576776 1107 94-95 0.28603644530306127 1107 96-97 0.6014762831375897 1107 98-99 0.7708375188352612 1107 100-101 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sequence quality scores pass #Quality Count 3 9.0 4 15.0 5 33.0 6 94.0 7 223.0 8 530.0 9 1002.0 10 1645.0 11 2737.0 12 3947.0 13 5289.0 14 7457.0 15 9390.0 16 11816.0 17 14619.0 18 13182.0 19 9661.0 20 9129.0 21 9661.0 22 10517.0 23 11647.0 24 13543.0 25 16381.0 26 20132.0 27 25342.0 28 31482.0 29 40013.0 30 50180.0 31 63200.0 32 80619.0 33 105532.0 34 140953.0 35 194952.0 36 281879.0 37 359446.0 38 217599.0 39 13385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.425147917370126 19.209471266933413 15.028382027961237 21.33699878773523 2 15.574423640529966 21.90216632827166 38.09667442499111 24.42673560620726 3 22.694377780091102 23.330679263650406 29.468895334933276 24.506047621325216 4 13.150970004535727 16.517707914791316 38.528150221552934 31.80317185912002 5 13.361440569962094 38.25963951990754 34.22827855085495 14.150641359275415 6 31.57860976648637 37.08247784065301 16.332900265073786 15.006012127786834 7 28.573446144895374 32.80646800293264 20.19776721333798 18.422318638834014 8 25.87600668676899 32.60677645856696 19.402939725113253 22.114277129550803 9 24.682667494955428 19.027289638850345 21.07167736352086 35.21836550267337 10-11 25.622041223058194 25.629102759991863 26.855531363774087 21.89332465317585 12-13 28.289847749582 23.18571557583923 26.446452118218367 22.077984556360395 14-15 23.258401582904273 25.53794803682903 27.22944769120875 23.974202689057947 16-17 21.986663598240195 28.36486441624968 27.265238647994277 22.383233337515847 18-19 21.557459005278407 28.79063672287551 28.371222586019567 21.280681685826515 20-21 23.39038430916235 25.65389274724137 27.856379635626233 23.09934330797005 22-23 22.295254737118114 25.978996907801506 28.385712585622052 23.34003576945833 24-25 23.892764121466925 25.999090725455922 28.235619142254762 21.872526010822394 26-27 23.181802580516656 25.042439376539257 30.122054352786144 21.653703690157947 28-29 21.62205350878131 28.53656313353113 28.447351822290845 21.394031535396717 30-31 22.54427580285279 25.92839854819874 28.04862369499232 23.478701953956147 32-33 22.656610041881972 26.396389248549774 28.50492111750529 22.442079592062964 34-35 24.225628456394116 25.77115024126007 28.129537429423234 21.873683872922577 36-37 23.487590540591867 25.271604191489573 29.52918188864615 21.711623379272414 38-39 22.576886157059437 26.69662138666853 29.168912661106727 21.557579795165307 40-41 22.780348720349597 27.068649043455554 28.067677812377962 22.083324423816883 42-43 22.088857287483282 27.403699108973544 26.909297181675473 23.598146421867696 44-45 23.24401001465511 25.439322798195246 27.544324752116466 23.772342435033174 46-47 24.578617505868028 27.10841763421487 27.17759888994891 21.13536596996819 48-49 24.11249916644462 26.618905232983302 27.297538894327122 21.971056706244955 50-51 21.622876994012863 27.26219758708302 29.217435144029192 21.897490274874926 52-53 23.318078820422702 28.329251020859463 26.20515434854902 22.147515810168812 54-55 22.37798324151243 26.68474009712945 27.127281219576705 23.80999544178142 56-57 23.716332233922202 26.088342462581526 27.108226114254542 23.087099189241734 58-59 25.646362426605673 25.953444012120087 26.19646469335449 22.20372886791975 60-61 23.327852450584835 26.773915274781302 28.784988458633965 21.1132438159999 62-63 22.43778644961441 26.955425696833966 28.26271913659268 22.344068716958944 64-65 22.031505497464778 28.762857269082705 27.035601195251523 22.170036038200998 66-67 23.230688030754205 26.52625946157638 26.284613670585216 23.958438837084202 68-69 22.578251996495688 26.872215835911774 26.963312193801737 23.586219973790808 70-71 24.330071220682836 26.625816407343482 26.517838931253685 22.526273440719997 72-73 23.719440334608624 25.582131399773978 28.523010813674343 22.175417451943055 74-75 22.33900748407222 28.95915635527202 26.715256962899236 21.986579197756523 76-77 23.010258741762396 26.636351462062013 26.431564874500342 23.92182492167525 78-79 23.23511337660481 27.058724883201897 26.88736847199603 22.81879326819726 80-81 24.448460151223063 26.5676146504764 26.49904061063255 22.484884587667985 82-83 23.632606467257542 26.298554305891532 27.82266857591294 22.246170650937987 84-85 22.889998086727516 27.54918235737679 27.38903019594161 22.17178935995408 86-87 22.847210400660664 27.778576563623293 26.5366537188327 22.837559316883347 88-89 22.367374944030413 28.034860293436832 25.440010311631344 24.157754450901407 90-91 23.360142149859023 26.078865142525554 26.216759961423218 24.344232746192205 92-93 24.348084922970965 27.192439512555517 26.61093064488993 21.84854491958359 94-95 23.645213887681358 26.750148069052937 26.87493405475671 22.729703988508998 96-97 21.541953558727723 27.010017059473494 28.759098456206278 22.6889309255925 98-99 22.727249705966766 28.249053184844204 25.788459265845436 23.235237843343594 100-101 21.822942708648416 27.200610474465503 26.43701091487601 24.53943590201007 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 107.0 1 81.0 2 68.5 3 86.5 4 81.0 5 79.5 6 148.5 7 327.0 8 558.5 9 721.5 10 751.0 11 743.5 12 779.5 13 877.5 14 1020.0 15 1180.5 16 1377.5 17 1522.0 18 1693.0 19 1960.5 20 2210.5 21 2505.5 22 2922.0 23 3550.5 24 4375.0 25 5492.5 26 7074.0 27 9261.0 28 11713.5 29 14615.5 30 18074.5 31 21841.5 32 26179.5 33 29343.5 34 31727.0 35 34388.5 36 38399.5 37 41947.0 38 43084.0 39 44663.0 40 46974.5 41 49235.5 42 50757.5 43 52153.0 44 54492.5 45 57573.5 46 61549.5 47 86644.0 48 132560.5 49 124998.5 50 78872.5 51 60179.5 52 56122.0 53 55247.5 54 54165.5 55 50547.5 56 45851.5 57 41856.0 58 39727.0 59 35797.0 60 29521.5 61 24168.0 62 19765.5 63 15784.5 64 11468.5 65 8097.5 66 5961.0 67 4602.5 68 3445.5 69 2555.5 70 2024.5 71 1562.0 72 1222.0 73 1064.0 74 858.0 75 715.0 76 546.5 77 333.0 78 218.0 79 168.0 80 121.5 81 87.0 82 71.0 83 44.5 84 22.0 85 12.0 86 6.5 87 2.5 88 2.5 89 4.0 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1829571791332747 2 0.012660072550655762 3 0.005401630954946459 4 0.013447810398252122 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004670160096464126 10-11 4.220024183551921E-4 12-13 2.532014510131153E-4 14-15 8.440048367103842E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014910752115216788 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.8133494557012808E-5 32-33 0.005851766867858664 34-35 5.345363965832434E-4 36-37 0.003854288754310755 38-39 1.6880096734207684E-4 40-41 0.006470703748112946 42-43 0.01381354582749329 44-45 0.0025601480046881656 46-47 0.0014348082224076531 48-49 0.012069269164958495 50-51 8.158713421533714E-4 52-53 0.003488553325069588 54-55 0.012997674485339917 56-57 0.0043606916563369855 58-59 0.001462941716964666 60-61 0.008721383312673971 62-63 0.005401630954946459 64-65 0.021184521401430643 66-67 0.0029821504230433575 68-69 1.1253397822805123E-4 70-71 0.001040939298609474 72-73 2.8133494557012808E-5 74-75 0.0 76-77 0.0014066747278506403 78-79 0.013672878354708224 80-81 0.01853997291307144 82-83 0.015445288511800031 84-85 0.010184325029638636 86-87 0.013419676903695109 88-89 0.03412592889765653 90-91 0.029512035790306434 92-93 0.0030384174121573836 94-95 0.011168997339134085 96-97 0.0125194050778707 98-99 0.012997674485339917 100-101 0.014770084642431723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1777241.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.07856857992995 #Duplication Level Percentage of deduplicated Percentage of total 1 77.7655956882181 42.05452099584407 2 12.951627961364451 14.008110018607717 3 4.103409681699823 6.657195656500573 4 1.7977722802511722 3.888838061946403 5 0.9282804576334713 2.5100039194770223 6 0.5578113928143568 1.8099384996586463 7 0.37380702298234564 1.4150464109607164 8 0.26248494021250823 1.1355847872384752 9 0.19089209256134113 0.9290854007050342 >10 0.9672707212035453 9.484819637402545 >50 0.06388627198426183 2.356422709025425 >100 0.03197176448970144 3.102351492846589 >500 0.0018683008505606538 0.6636317926585317 >1k 0.002179684325654096 2.858393420396098 >5k 7.265614418846987E-4 3.0682095111478183 >10k+ 4.1517796679125645E-4 4.057847685584305 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35086 1.9741835800547027 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13530 0.7612923627127666 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13507 0.7599982219631439 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10170 0.5722352792896405 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9635 0.5421324401136368 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8863 0.49869432451760903 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8474 0.4768064657522531 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8406 0.47298031049249933 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7302 0.41086155451061507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6580 0.37023678837028856 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5402 0.3039542751939664 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4670 0.26276683916249965 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4306 0.2422856551249943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3887 0.21870978668621757 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3351 0.18855068052109986 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3327 0.18720027278236323 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2882 0.16216146262662184 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2819 0.15861664231243822 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2809 0.15805397242129796 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2735 0.15389021522686008 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2685 0.15107686577115878 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2281 0.12834500216909242 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2217 0.12474391486579478 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2109 0.11866708004148004 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1809 0.10178698330727234 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.6266989114025615E-5 2 0.0 0.0 0.0 0.0 5.6266989114025615E-5 3 0.0 0.0 0.0 0.0 5.6266989114025615E-5 4 0.0 0.0 0.0 0.0 5.6266989114025615E-5 5 0.0 0.0 0.0 0.0 5.6266989114025615E-5 6 0.0 0.0 0.0 0.0 5.6266989114025615E-5 7 1.6880096734207684E-4 0.0 0.0 0.0 5.6266989114025615E-5 8 1.6880096734207684E-4 0.0 0.0 0.0 5.6266989114025615E-5 9 1.6880096734207684E-4 0.0 0.0 0.0 5.6266989114025615E-5 10-11 2.2506795645610246E-4 0.0 0.0 0.0 5.6266989114025615E-5 12-13 2.2506795645610246E-4 0.0 0.0 0.0 5.6266989114025615E-5 14-15 2.8133494557012806E-4 0.0 0.0 2.8133494557012808E-5 5.6266989114025615E-5 16-17 2.8133494557012806E-4 0.0 0.0 5.6266989114025615E-5 1.6880096734207684E-4 18-19 3.657354292411665E-4 0.0 0.0 5.6266989114025615E-5 2.2506795645610246E-4 20-21 4.501359129122049E-4 0.0 0.0 1.1253397822805123E-4 2.5320145101311525E-4 22-23 5.064029020262305E-4 0.0 0.0 2.2506795645610243E-4 2.8133494557012806E-4 24-25 5.064029020262305E-4 0.0 0.0 4.220024183551921E-4 3.376019346841537E-4 26-27 5.064029020262305E-4 0.0 0.0 5.908033856972689E-4 3.938689237981793E-4 28-29 7.31470858482333E-4 0.0 0.0 0.0010690727931664866 3.938689237981793E-4 30-31 7.877378475963586E-4 0.0 0.0 0.0021100120917759606 4.220024183551921E-4 32-33 8.440048367103842E-4 0.0 0.0 0.003601087303297639 4.501359129122049E-4 34-35 0.0010409392986094738 0.0 0.0 0.005879900362415677 4.501359129122049E-4 36-37 0.001294140749622589 0.0 0.0 0.009171519225586174 4.501359129122049E-4 38-39 0.0013504077387366147 0.0 0.0 0.014713817653317699 4.501359129122049E-4 40-41 0.0013504077387366147 0.0 0.0 0.020087315113707142 5.626698911402561E-4 42-43 0.0015192087060786916 0.0 0.0 0.025545213057767628 5.626698911402561E-4 44-45 0.0018005436516488197 0.0 0.0 0.031003111001828114 5.908033856972689E-4 46-47 0.0021100120917759606 0.0 0.0 0.037670749211840146 6.189368802542817E-4 48-49 0.0022506795645610245 0.0 0.0 0.04293171269400155 7.877378475963586E-4 50-51 0.0022506795645610245 0.0 0.0 0.04867094558363216 7.877378475963586E-4 52-53 0.002278813059118037 0.0 0.0 0.05823633373301651 8.440048367103842E-4 54-55 0.00230694655367505 0.0 0.0 0.07593230180937757 9.002718258244098E-4 56-57 0.00230694655367505 0.0 0.0 0.10718861426221879 9.002718258244098E-4 58-59 0.00230694655367505 0.0 0.0 0.14643483916925165 9.002718258244098E-4 60-61 0.00230694655367505 0.0 0.0 0.1897604207870514 9.002718258244098E-4 62-63 0.00230694655367505 0.0 0.0 0.2139833596006394 9.565388149384355E-4 64-65 0.00230694655367505 0.0 0.0 0.2453522060317087 9.565388149384355E-4 66-67 0.002475747521017127 5.6266989114025615E-5 0.0 0.3220441121941256 9.565388149384355E-4 68-69 0.002475747521017127 5.6266989114025615E-5 0.0 0.4216366829259509 9.565388149384355E-4 70-71 0.0025038810155741395 5.6266989114025615E-5 0.0 0.5175437658708076 9.565388149384355E-4 72-73 0.0025320145101311526 5.6266989114025615E-5 0.0 0.6261953218499912 9.565388149384355E-4 74-75 0.0025320145101311526 5.6266989114025615E-5 0.0 0.7283761740810616 9.565388149384355E-4 76-77 0.0025320145101311526 5.6266989114025615E-5 0.0 0.8188816260709718 9.565388149384355E-4 78-79 0.0025320145101311526 5.6266989114025615E-5 0.0 0.9017066340468175 9.565388149384355E-4 80-81 0.00320721837949946 5.6266989114025615E-5 0.0 0.961940445893382 9.565388149384355E-4 82-83 0.0032634853686134857 5.6266989114025615E-5 0.0 1.0529241672907614 0.001012805804052461 84-85 0.0033197523577275113 5.6266989114025615E-5 0.0 1.2665980584512737 0.001012805804052461 86-87 0.0034041528413985495 5.6266989114025615E-5 0.0 1.578992382012344 0.001012805804052461 88-89 0.0034604198305125755 5.6266989114025615E-5 0.0 1.9450372796936377 0.001012805804052461 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 6000 0.0 22.476803 12-13 GTACTTT 6450 0.0 20.79822 14-15 TGAACCG 230 9.0525646E-8 20.646755 5 TACATGG 5830 0.0 20.539059 2 GTACATG 5890 0.0 20.495535 1 TAGCCCT 965 0.0 19.690048 4 AGTACTT 6410 0.0 19.520458 12-13 GTGTAGC 1035 0.0 19.516851 1 ACATGGG 5850 0.0 19.490839 3 ATACCGT 220 1.3489826E-6 19.42672 6 GTATAGC 605 0.0 19.07905 1 AGAGTAC 9895 0.0 18.716139 10-11 CGATACA 155 8.8548206E-4 18.617458 1 ACGACGG 130 0.0075761685 18.264437 6 ACTTTTT 7495 0.0 18.152851 16-17 TAAGGTG 635 0.0 17.948044 5 GTAAGGT 585 0.0 17.864088 4 TCGCGTA 135 0.009416583 17.587976 9 ACCGGTC 165 0.0014668141 17.268196 8 TACTTTT 7195 0.0 17.027714 14-15 >>END_MODULE