FastQCFastQC Report
Thu 26 May 2016
SRR936565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936565_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884869
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT362421.9227861458806954No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT141840.752519140587489No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT140270.7441896492541391No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC106540.5652382207994295No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT100810.5348382301369485No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC92610.4913338804977959No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT90030.4776459265869405No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC87370.4635335399966788No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG75920.4027866127566425No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA71090.3771614897374831No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC56560.3000739043403017No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA49840.2644215592701668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44590.23656816468412395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.20966974362674543No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT37020.19640622239529643No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC36520.19375351814900665No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA31450.16685509709162813No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG30050.1594275252020167No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC29080.15428127896421448No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG28740.15247744007673744No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT27890.14796784285804476No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC24380.1293458590490904No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC23080.12244882800873695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.11846977163930225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG4300.025.4135071
GTACATG61450.022.8089521
TACATGG62650.022.0616932
GAGTACT62900.022.05127312-13
ACATGGG61050.021.7835043
GTACTTT64800.021.14814614-15
GTAAGGT6650.020.7113654
AGTACTT66700.019.08580212-13
CATGGGG29500.018.3533444
AGAGTAC103300.017.70361310-11
ACTTTTT79850.017.63667516-17
TATACTG8400.017.5272885
GTAAGCG3050.017.13009694-95
TACTTTT72900.017.0390614-15
CCGATCG3401.7538696E-816.7627879
GCGTTCG1002.7609267E-416.62618428-29
GGACCGT2002.76448E-416.6226566
AAGGTGT14500.016.3776
GGTGTGC13750.016.2340748
GGTAAGG7400.016.0458873
Produced by FastQC (version 0.11.5)