##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936565_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884869 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01295421591633 31.0 31.0 34.0 31.0 34.0 2 32.15052398866977 33.0 31.0 34.0 30.0 34.0 3 32.27464348981282 34.0 31.0 34.0 30.0 34.0 4 35.96599551480766 37.0 35.0 37.0 35.0 37.0 5 35.77747525159573 37.0 35.0 37.0 35.0 37.0 6 35.8960813722333 37.0 35.0 37.0 35.0 37.0 7 35.8529266490138 37.0 35.0 37.0 35.0 37.0 8 35.829439605617154 37.0 35.0 37.0 35.0 37.0 9 37.48996349348416 39.0 37.0 39.0 35.0 39.0 10-11 37.34990707577025 39.0 37.0 39.0 34.0 39.0 12-13 37.28543336433461 39.0 37.0 39.0 34.0 39.0 14-15 38.3708188738846 40.0 38.0 41.0 34.0 41.0 16-17 38.334485049093594 40.0 38.0 41.0 33.5 41.0 18-19 38.505211502762265 40.0 38.0 41.0 34.0 41.0 20-21 38.52352258963355 40.0 38.0 41.0 34.0 41.0 22-23 38.432992956009144 40.0 38.0 41.0 34.0 41.0 24-25 38.2927863952349 40.0 38.0 41.0 34.0 41.0 26-27 38.06984782496821 40.0 37.5 41.0 33.0 41.0 28-29 38.02908849368312 40.0 37.5 41.0 33.5 41.0 30-31 38.07261300387454 40.0 37.0 41.0 33.0 41.0 32-33 37.94760298991601 40.0 37.0 41.0 33.0 41.0 34-35 37.75217561538759 40.0 37.0 41.0 33.0 41.0 36-37 37.49974401404023 40.0 37.0 41.0 32.5 41.0 38-39 37.33830839172377 39.0 36.0 41.0 32.0 41.0 40-41 37.20195010899962 39.0 36.0 41.0 31.5 41.0 42-43 36.96811661712299 39.0 36.0 41.0 31.0 41.0 44-45 36.737081197685356 39.0 36.0 41.0 31.0 41.0 46-47 36.43982499579546 39.0 35.5 40.0 30.0 41.0 48-49 36.09510209993374 39.0 35.0 40.0 29.5 41.0 50-51 35.78558854753301 38.5 35.0 40.0 28.5 41.0 52-53 35.573078818740186 38.0 35.0 40.0 28.0 41.0 54-55 35.34206196823227 38.0 34.0 40.0 28.0 41.0 56-57 34.959536975779216 38.0 34.0 40.0 26.5 41.0 58-59 34.61783577532444 38.0 34.0 40.0 26.0 41.0 60-61 34.161513346550876 37.0 33.0 40.0 26.0 41.0 62-63 33.822743384288245 36.5 33.0 39.0 25.0 40.5 64-65 33.440688451027626 36.0 32.5 39.0 24.5 40.0 66-67 32.91785052436005 35.5 32.0 38.5 23.0 40.0 68-69 32.58735302028947 35.0 31.5 38.0 22.5 40.0 70-71 32.08758566245187 35.0 31.0 37.5 21.5 39.5 72-73 31.60040618207419 34.5 31.0 37.0 20.5 39.0 74-75 31.104744945139423 34.0 30.0 36.0 19.5 39.0 76-77 29.719781586943178 32.5 28.5 35.0 18.0 37.0 78-79 30.507342950624157 34.0 30.0 35.0 18.5 37.0 80-81 30.24676489453644 34.0 30.0 35.0 17.5 37.0 82-83 29.882366095468704 34.0 29.5 35.0 13.5 36.5 84-85 29.451338262765212 34.0 29.0 35.0 8.5 36.0 86-87 29.170873678754333 34.0 29.0 35.0 7.0 36.0 88-89 28.75130844636948 33.5 29.0 35.0 2.0 35.0 90-91 28.215144129379812 33.0 27.5 35.0 2.0 35.0 92-93 27.727782408220413 33.0 27.0 35.0 2.0 35.0 94-95 27.104395583990186 32.5 26.0 34.0 2.0 35.0 96-97 26.297119322350785 32.0 25.0 34.0 2.0 35.0 98-99 25.385270806618394 32.0 22.0 34.0 2.0 35.0 100-101 22.078570447070856 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16803547886487635 1101 2 0.22818365065310786 1101 3 0.21998903494620237 1101 4 0.15790637589746126 1101 5 0.06341197053068726 1101 6 0.09301278029057158 1101 7 0.14111621227731064 1101 8 0.15211587742971489 1101 9 0.08160267738991678 1101 10-11 0.1842280255185571 1101 12-13 0.16026561440489928 1101 14-15 0.29586332571249585 1101 16-17 0.395731658989142 1101 18-19 0.17917652718957555 1101 20-21 0.1863709641323581 1101 22-23 0.15073096740810144 1101 24-25 0.11552907874321505 1101 26-27 0.0788899674801371 1101 28-29 0.040347082918067656 1101 30-31 0.20287628608208053 1101 32-33 0.14068311360872343 1101 34-35 0.14210735480633474 1101 36-37 0.09503212445659415 1101 38-39 0.14702531677466624 1101 40-41 0.06775761903305266 1101 42-43 0.1965601462189568 1101 44-45 0.20117030233962652 1101 46-47 0.14926505490603859 1101 48-49 0.13933156940667857 1101 50-51 0.21804527039082444 1101 52-53 0.20209488556177746 1101 54-55 0.29549169885470405 1101 56-57 0.3374805321387484 1101 58-59 0.1520053527274925 1101 60-61 0.09414263821409463 1101 62-63 0.14482629012695014 1101 64-65 0.1110331693806188 1101 66-67 0.06847799248829034 1101 68-69 -0.03521796777121722 1101 70-71 0.0024081912068254496 1101 72-73 0.05903039517214026 1101 74-75 0.032946766337470734 1101 76-77 -0.07794007429853522 1101 78-79 -0.0019546412849500427 1101 80-81 -2.960126458297907E-4 1101 82-83 0.2568774690187716 1101 84-85 0.208256925493707 1101 86-87 0.10764452862239793 1101 88-89 0.07597241396636178 1101 90-91 -0.0014747663351606377 1101 92-93 0.021424234582568147 1101 94-95 -0.04490936508359411 1101 96-97 0.1236778654503361 1101 98-99 0.3048914188597003 1101 100-101 0.5388314657829518 1102 1 0.16510511650726656 1102 2 0.19752212648850076 1102 3 0.13344390186254174 1102 4 0.08519817492329906 1102 5 0.036690569864155975 1102 6 0.10908902056566205 1102 7 0.09400923541647899 1102 8 0.15914606507322304 1102 9 0.17230911311072816 1102 10-11 0.1855048561155357 1102 12-13 0.15311290745089678 1102 14-15 0.31822532037912055 1102 16-17 0.21318345935249994 1102 18-19 0.13773704807167064 1102 20-21 0.05389136486019197 1102 22-23 -0.036895644587261245 1102 24-25 0.10979531433697076 1102 26-27 -0.21192663983389082 1102 28-29 -0.19249281194694845 1102 30-31 -0.010462662066558437 1102 32-33 -0.02459735198903701 1102 34-35 0.07389361843236486 1102 36-37 0.21553243515232623 1102 38-39 0.2345270060956537 1102 40-41 0.12840493896885619 1102 42-43 0.12204036618597058 1102 44-45 0.20033835529444133 1102 46-47 0.22382097247553645 1102 48-49 0.0766219716949621 1102 50-51 -0.017704000937108333 1102 52-53 0.03811511004292356 1102 54-55 0.03601626262673818 1102 56-57 0.2927287647955197 1102 58-59 0.05639344235276411 1102 60-61 0.19183824765144664 1102 62-63 -0.0214608017888267 1102 64-65 0.09634191107682 1102 66-67 0.14947962747235266 1102 68-69 0.08172849921422198 1102 70-71 0.13433832576733806 1102 72-73 0.21141785852954698 1102 74-75 0.14343201595293564 1102 76-77 -0.21785521117184814 1102 78-79 -0.16694313300344632 1102 80-81 0.19809018076017182 1102 82-83 0.04583521370211585 1102 84-85 0.006435489159862584 1102 86-87 0.1466645343626034 1102 88-89 5.843751948795273E-4 1102 90-91 0.22664800445776834 1102 92-93 0.2988088243527365 1102 94-95 0.4609941151266206 1102 96-97 0.46393236426885665 1102 98-99 0.6147114069055775 1102 100-101 0.9286512518069365 1103 1 0.047962814606979265 1103 2 0.07522741050184578 1103 3 0.08449893046429935 1103 4 0.05929052259723733 1103 5 -0.10767842403630112 1103 6 7.799847376190883E-4 1103 7 0.04903424841728565 1103 8 0.023654181094542537 1103 9 0.08951567418199602 1103 10-11 0.01362316950527287 1103 12-13 0.03854748971888711 1103 14-15 0.016632535959502093 1103 16-17 0.1755222697249721 1103 18-19 0.007984560991218359 1103 20-21 0.05065496085486387 1103 22-23 0.07470455773771789 1103 24-25 -0.10341123183754775 1103 26-27 0.050526198403638034 1103 28-29 -0.025379440216106275 1103 30-31 0.006240321075729582 1103 32-33 0.06170537235604456 1103 34-35 -0.03855248241051612 1103 36-37 -0.02221684844933236 1103 38-39 0.03098718233776765 1103 40-41 0.06352247434187319 1103 42-43 -0.0322755952774898 1103 44-45 0.13496277432111015 1103 46-47 0.005377286937843451 1103 48-49 0.010102811086312613 1103 50-51 0.11784306873635586 1103 52-53 0.07013703264482274 1103 54-55 -0.06132360067524445 1103 56-57 -0.17220216505973696 1103 58-59 0.1173135402379657 1103 60-61 0.045064359355407646 1103 62-63 -0.061053913936994775 1103 64-65 -0.12451894174868983 1103 66-67 0.06802122529796861 1103 68-69 -0.002067254128064633 1103 70-71 0.13811626056290294 1103 72-73 -0.014240764116760118 1103 74-75 -0.02816857910459447 1103 76-77 -0.023779987991421336 1103 78-79 0.10495983226274674 1103 80-81 0.22349523698864004 1103 82-83 0.1581100865380236 1103 84-85 0.0022489940134917674 1103 86-87 -0.25992995302661726 1103 88-89 -0.4106410553212534 1103 90-91 -0.2777895348154864 1103 92-93 0.050954464479712414 1103 94-95 0.18271745603472667 1103 96-97 0.034372163785658216 1103 98-99 0.2867293649400722 1103 100-101 0.5599050944918211 1104 1 0.16448677873244932 1104 2 0.20197506064350534 1104 3 0.1418806384825686 1104 4 0.08834295340431453 1104 5 0.051925205107330896 1104 6 0.1121657888312626 1104 7 0.14588784412178768 1104 8 0.11472880158221699 1104 9 0.10570906869589436 1104 10-11 0.16123787438113624 1104 12-13 0.0928706037089384 1104 14-15 0.1570058902769702 1104 16-17 0.11421020541068572 1104 18-19 0.06573071201792402 1104 20-21 0.017126812062052466 1104 22-23 0.1414823117751638 1104 24-25 0.04932000620905086 1104 26-27 0.1822185827954641 1104 28-29 0.18619066989848676 1104 30-31 0.05872662840645404 1104 32-33 0.10381582394922617 1104 34-35 0.11121318316698847 1104 36-37 0.18662011281166713 1104 38-39 0.1746549416386216 1104 40-41 0.18562443152936936 1104 42-43 0.16062332476913355 1104 44-45 0.09437045889204398 1104 46-47 0.18617228687703857 1104 48-49 0.23982133184374987 1104 50-51 0.34447850239400424 1104 52-53 0.3954199623635972 1104 54-55 0.23809942246462867 1104 56-57 0.22125644772912523 1104 58-59 0.04766558037186286 1104 60-61 0.1875690451753158 1104 62-63 0.15263997130688267 1104 64-65 0.33899975723493014 1104 66-67 0.15510938124761253 1104 68-69 0.20177451020689574 1104 70-71 0.24087129684217956 1104 72-73 0.10997801981091726 1104 74-75 0.22026655681458251 1104 76-77 0.10624330648304792 1104 78-79 0.1390403779892253 1104 80-81 0.3949186839802472 1104 82-83 0.37795494638033134 1104 84-85 0.21719810574879972 1104 86-87 0.23346951160000273 1104 88-89 0.19281681051577593 1104 90-91 0.39088365291435423 1104 92-93 0.3640871804486707 1104 94-95 0.11937550669890484 1104 96-97 0.1383476846108529 1104 98-99 0.33159754531330776 1104 100-101 0.6595325739810427 1105 1 -0.05674763940288585 1105 2 0.09038387284105909 1105 3 0.013339443027362563 1105 4 -0.01660923103281675 1105 5 -0.07195026468695431 1105 6 -0.06219728444058603 1105 7 -0.015798861432223532 1105 8 -0.0035420153354763784 1105 9 -0.05040283918379629 1105 10-11 -0.13941270448856358 1105 12-13 -0.1789665871810655 1105 14-15 -0.017377873783793518 1105 16-17 0.04363175141887865 1105 18-19 -0.13881011308160396 1105 20-21 0.0022592047675615845 1105 22-23 0.004946630084525339 1105 24-25 -0.006917841495400978 1105 26-27 -0.07189217622580912 1105 28-29 -0.04228269000219598 1105 30-31 0.062419509074459256 1105 32-33 -0.019263097711103683 1105 34-35 0.007214381154220462 1105 36-37 -0.03676028970448897 1105 38-39 0.12066700465199887 1105 40-41 0.16903759918522354 1105 42-43 -0.0044502456296271475 1105 44-45 0.03252824174646207 1105 46-47 0.039787128313790276 1105 48-49 -0.10354419975836038 1105 50-51 0.14030803296818561 1105 52-53 0.022511101982019 1105 54-55 0.0030610358548770478 1105 56-57 -0.01972510818041684 1105 58-59 -0.007420763518496187 1105 60-61 -0.12391651567733675 1105 62-63 0.037180456283856245 1105 64-65 -0.0028865246775353626 1105 66-67 0.37904682893015007 1105 68-69 0.3546890770891622 1105 70-71 0.3911145789492707 1105 72-73 0.2846625088995971 1105 74-75 -0.013643851124637507 1105 76-77 -0.17645579275904666 1105 78-79 0.06510765423109888 1105 80-81 0.40238446859575205 1105 82-83 0.3011595244299521 1105 84-85 0.15098984870684617 1105 86-87 0.04836867470955397 1105 88-89 0.1756929624447423 1105 90-91 0.3209191201388215 1105 92-93 0.1702657901410305 1105 94-95 0.2128426026758241 1105 96-97 0.24480358866605556 1105 98-99 0.2657775948578234 1105 100-101 0.4789275904297483 1106 1 0.05396716666740886 1106 2 0.10016012834208254 1106 3 0.05675794808855272 1106 4 0.021990670131735612 1106 5 -0.08516516141720842 1106 6 0.005349431190325049 1106 7 -0.05648812606941078 1106 8 -0.056774112782171926 1106 9 -0.057257376183898145 1106 10-11 -0.027035119579871036 1106 12-13 0.0021158005169894523 1106 14-15 0.133069385613787 1106 16-17 0.08305005992497172 1106 18-19 -0.03591459477375736 1106 20-21 -0.04339704569513003 1106 22-23 -0.019721463251677562 1106 24-25 -0.11878665770802144 1106 26-27 0.001184797194831333 1106 28-29 0.05288988687155438 1106 30-31 0.04498908496393028 1106 32-33 2.572263215512294E-4 1106 34-35 -0.057937516664601674 1106 36-37 -0.055524649116208025 1106 38-39 0.15412611035635848 1106 40-41 0.13657779159034789 1106 42-43 0.08617592181857958 1106 44-45 0.12046872846923407 1106 46-47 -0.02867873987284497 1106 48-49 0.10602794744080768 1106 50-51 0.019689725577912043 1106 52-53 -0.16608050567342048 1106 54-55 -0.21287243668352573 1106 56-57 -0.16006533732159767 1106 58-59 -0.14192206183354728 1106 60-61 -0.1618196762446047 1106 62-63 -0.07859639446152045 1106 64-65 -0.02640189535019033 1106 66-67 -0.08108231377421049 1106 68-69 -0.17394316339642302 1106 70-71 -0.2878900325851177 1106 72-73 -0.293302484981659 1106 74-75 -0.1702010556305531 1106 76-77 -0.4198185714472409 1106 78-79 -0.24935098991212712 1106 80-81 -0.1382675664468458 1106 82-83 0.07520828683025016 1106 84-85 -0.1255408509026843 1106 86-87 -0.15691530984842572 1106 88-89 -0.16408051853951378 1106 90-91 0.05699777307552978 1106 92-93 -0.024093722428087716 1106 94-95 0.2298438671945604 1106 96-97 0.29972603845492074 1106 98-99 0.7261806486716509 1106 100-101 0.7129311494502453 1107 1 -5.09297396636299E-4 1107 2 0.05677705992063409 1107 3 0.07632763227826445 1107 4 0.032418507388989326 1107 5 -0.008972672407992377 1107 6 0.029681429400689296 1107 7 -0.0053619037719983 1107 8 -0.06786478723205391 1107 9 0.0012128917243288129 1107 10-11 0.06313590965005034 1107 12-13 0.012691942777316001 1107 14-15 0.14754057509927776 1107 16-17 0.09568693875320378 1107 18-19 0.020235176045119374 1107 20-21 0.06276802049448094 1107 22-23 0.010198277436799685 1107 24-25 0.10682867766423954 1107 26-27 0.042268684536544754 1107 28-29 0.024333690500306204 1107 30-31 -0.06791286071153735 1107 32-33 -0.01769527571310192 1107 34-35 0.013241514912799346 1107 36-37 0.07114528449349677 1107 38-39 0.17126214119291916 1107 40-41 0.06392432709250073 1107 42-43 -0.09816424904057186 1107 44-45 0.2116438778116887 1107 46-47 0.15073244391174967 1107 48-49 -0.005400132229368637 1107 50-51 0.27505234605020945 1107 52-53 0.1391119155327516 1107 54-55 0.28891255235573254 1107 56-57 0.11175448978742253 1107 58-59 0.25137668362857823 1107 60-61 0.3240524580843527 1107 62-63 0.4452117741508417 1107 64-65 0.37016228137223095 1107 66-67 0.3220424318226165 1107 68-69 0.2560898696154936 1107 70-71 0.25996327517201934 1107 72-73 0.3730664756429114 1107 74-75 0.19598089102454352 1107 76-77 0.21200450748707667 1107 78-79 0.35968323384837575 1107 80-81 0.5861850259540873 1107 82-83 0.6302014373614178 1107 84-85 0.5379215357511384 1107 86-87 0.5715509284269196 1107 88-89 0.4967294988965243 1107 90-91 0.4693626686917085 1107 92-93 0.3695299787976509 1107 94-95 0.6647411455709893 1107 96-97 0.912638642135196 1107 98-99 1.1530907728918294 1107 100-101 1.465513731413136 1108 1 -0.023801745171724065 1108 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2316 76-77 -0.7433830769751033 2316 78-79 -1.066510851383164 2316 80-81 -1.4591242722571884 2316 82-83 -1.1781041032584803 2316 84-85 -1.152219184992802 2316 86-87 -1.3610101866349176 2316 88-89 -1.198124161936562 2316 90-91 -1.305424363963283 2316 92-93 -1.3255517949638822 2316 94-95 -1.57409324537214 2316 96-97 -1.3902694647474014 2316 98-99 -1.5991226813418749 2316 100-101 -1.8426646668345619 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 16.0 5 53.0 6 91.0 7 198.0 8 525.0 9 1054.0 10 1780.0 11 2813.0 12 4189.0 13 5836.0 14 7691.0 15 10108.0 16 12749.0 17 15818.0 18 14126.0 19 10504.0 20 10091.0 21 10543.0 22 11476.0 23 12883.0 24 14904.0 25 18124.0 26 22460.0 27 27932.0 28 34794.0 29 43391.0 30 54255.0 31 67945.0 32 86552.0 33 113106.0 34 151823.0 35 209456.0 36 299717.0 37 377871.0 38 218178.0 39 11810.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33015626343461 19.184598057667056 15.018687709845308 21.466557969053024 2 15.730976745568 21.813823411905712 38.02926528007029 24.425934562455996 3 22.560317576537027 23.317225301282807 29.467299527688727 24.655157594491435 4 13.125034485393048 16.522429299638805 38.34292911500932 32.00960709995883 5 13.439059794234492 38.231670826117956 34.138218990866186 14.191050388781365 6 31.596622783794707 37.00082658395875 16.399786510022462 15.002764122224074 7 28.60941529623544 32.773099881211905 20.244377725985203 18.373107096567452 8 25.967481029185585 32.672297119852885 19.338320063622458 22.02190178733907 9 24.697797358697983 18.952445528186125 21.058579350476542 35.29117776263935 10-11 25.642719978481164 25.642773032805344 26.851642336395713 21.86286465231777 12-13 28.23187521553439 23.132503912778205 26.51508077565854 22.12054009602886 14-15 23.20574188064842 25.563316988695988 27.12683664948021 24.10410448117537 16-17 22.0659962171153 28.329110969039238 27.21546791312629 22.389424900719174 18-19 21.602986732764982 28.76298034505316 28.301887292963062 21.332145629218793 20-21 23.417197693845036 25.671677978681807 27.836152008441967 23.07497231903119 22-23 22.34572790791384 25.969609678469325 28.365511513290098 23.319150900326736 24-25 23.924552266501205 25.98550710412926 28.182520189224153 21.90742044014538 26-27 23.25175451278861 25.070744237067146 29.98022318225047 21.697278067893773 28-29 21.683304373201047 28.441458529532703 28.38107996975766 21.49415712750859 30-31 22.604770523631778 25.927005688264636 27.97221310712204 23.496010680981545 32-33 22.701735537738163 26.399591482137293 28.428174468959938 22.470498511164607 34-35 24.230120993019828 25.808864084410455 28.045883273982454 21.915131648587266 36-37 23.550732423623796 25.30910624818436 29.39608710820344 21.744074219988406 38-39 22.636020826734114 26.71628748024214 29.046799664454635 21.60089202856911 40-41 22.793134375632516 27.027287999853566 28.04490780249459 22.13466982201933 42-43 22.19280942416821 27.345590326263654 26.887226398196994 23.57437385137114 44-45 23.330532926969347 25.453359439805546 27.456096534172165 23.760011099052946 46-47 24.527884460813414 27.105917818074165 27.160377894037087 21.20581982707533 48-49 24.08333992091716 26.62008139600893 27.252436389141042 22.04414229393286 50-51 21.63716379861474 27.22088553700702 29.15162785268815 21.990322811690085 52-53 23.404406583984855 28.30085270922997 26.11353044541218 22.181210261372993 54-55 22.428180480201018 26.638030469112273 27.12296410111662 23.810824949570087 56-57 23.75165232726465 26.11548504211097 27.052248159858532 23.080614470765845 58-59 25.51490021383602 25.97623531352934 26.221904013331525 22.286960459303113 60-61 23.295788106230205 26.792245410438863 28.730707103987786 21.18125937934315 62-63 22.52365560704474 26.905845537401696 28.16383464367233 22.406664211881232 64-65 22.14680116529159 28.65642601642667 26.977704685441044 22.219068132840693 66-67 23.24811347132418 26.51241954763634 26.239527464557455 23.999939516482023 68-69 22.67855650658358 26.770895093783036 26.95178937846273 23.598759021170654 70-71 24.30190034628438 26.617925827127326 26.512772519228143 22.56740130736015 72-73 23.767116746690718 25.569753561291346 28.409024590816244 22.254105101201688 74-75 22.39530123981796 28.886259601724895 26.66798425788001 22.050454900577133 76-77 23.09022988017878 26.611888319659084 26.430437476920726 23.86744432324141 78-79 23.28643422173446 27.031810697263168 26.802023885413263 22.879731195589113 80-81 24.44485887789402 26.585599239376084 26.489460111414214 22.48008177131568 82-83 23.683835636934337 26.298058671989345 27.734125155556406 22.283980535519916 84-85 22.936923467097632 27.46058114622673 27.303027762715427 22.29946762396021 86-87 22.873650882919783 27.720113168656052 26.495047876496013 22.91118807192815 88-89 22.473598184086725 27.94953921678128 25.42270629125553 24.154156307876466 90-91 23.426636506564492 26.104486394790644 26.166780483378783 24.302096615266084 92-93 24.309187227596965 27.157060660160386 26.53825646140664 21.995495650836013 94-95 23.673949916888922 26.781211219915672 26.785084254416986 22.75975460877842 96-97 21.675105008411816 26.9810472881382 28.586931897462343 22.75691580598764 98-99 22.753224345700158 28.116018614166695 25.770757761694735 23.359999278438416 100-101 21.959441983062757 27.09765031118992 26.344300387074043 24.59860731867328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 123.0 1 93.5 2 75.5 3 95.0 4 90.5 5 91.5 6 151.5 7 315.0 8 545.5 9 726.0 10 762.5 11 747.5 12 814.0 13 907.5 14 1050.0 15 1231.0 16 1396.0 17 1530.0 18 1687.0 19 1931.5 20 2216.0 21 2588.0 22 3071.5 23 3779.5 24 4703.5 25 5773.5 26 7339.0 27 9600.5 28 12063.5 29 15168.5 30 18982.0 31 22983.0 32 27466.0 33 30707.0 34 33078.5 35 36048.0 36 40670.5 37 44519.5 38 45829.5 39 47544.5 40 49788.5 41 51968.5 42 53375.0 43 55030.0 44 57968.0 45 61125.5 46 64905.5 47 91179.5 48 139122.5 49 131840.0 50 84168.0 51 64276.5 52 60026.5 53 58859.0 54 57750.0 55 54360.0 56 49300.5 57 44985.5 58 42970.0 59 38674.5 60 31569.0 61 25850.5 62 21387.0 63 16991.5 64 12254.5 65 8725.5 66 6435.5 67 4957.0 68 3777.5 69 2882.5 70 2172.0 71 1653.0 72 1325.5 73 1170.0 74 995.5 75 809.5 76 611.5 77 370.0 78 240.5 79 181.0 80 122.5 81 94.5 82 73.0 83 52.0 84 33.5 85 12.5 86 5.0 87 3.0 88 1.5 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03941918509986636 2 0.00583594934183755 3 0.005040138067950611 4 6.897031040353468E-4 5 2.6527042462897956E-4 6 1.591622547773877E-4 7 0.0 8 0.0 9 0.002175217481957632 10-11 4.5095972186926517E-4 12-13 0.0010080276135901222 14-15 0.002705758331215591 16-17 0.0033954614352509375 18-19 0.0 20-21 0.0 22-23 0.0013528791656077955 24-25 7.692842314240406E-4 26-27 0.004589178346081345 28-29 0.004987083983024815 30-31 0.0012467709957562037 32-33 3.448515520176734E-4 34-35 0.009204883734625588 36-37 0.010690398112547874 38-39 0.009337518946940078 40-41 0.0015650955053109793 42-43 6.897031040353468E-4 44-45 0.0012467709957562037 46-47 1.061081698515918E-4 48-49 5.305408492579591E-4 50-51 0.0012732980382191017 52-53 0.002811866501067183 54-55 0.003979056369434693 56-57 0.0014589873354593874 58-59 7.162301464982446E-4 60-61 0.004005583411897591 62-63 0.006101219766466529 64-65 0.01010680317836412 66-67 0.0030771369256961624 68-69 0.0035280966475654276 70-71 1.061081698515918E-4 72-73 0.0010080276135901222 74-75 0.006180800893855223 76-77 0.00323629918047355 78-79 0.004164745666674978 80-81 0.005331935535042488 82-83 0.005438043704894081 84-85 0.0058890034267633454 86-87 0.0037933670721944073 88-89 0.01047818177284469 90-91 0.013794062080706935 92-93 0.0012732980382191017 94-95 0.0022282715668834278 96-97 0.0010610816985159182 98-99 0.0036076777749541214 100-101 0.004642232431007142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1884869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.140174703374576 #Duplication Level Percentage of deduplicated Percentage of total 1 76.97906496712179 40.90680960855273 2 13.25585456186812 14.088368545203933 3 4.3573541030415255 6.946516748402781 4 1.8530325787139035 3.938818998556061 5 0.9937606402396427 2.640430701783599 6 0.5884144372318326 1.8761067595492449 7 0.39166914076714443 1.4569356596400664 8 0.25725724316346454 1.0936555876332017 9 0.1925511548942933 0.9208981809377358 >10 1.0246754963388098 9.91931910726978 >50 0.06809827750833654 2.483091265315449 >100 0.032914597721806306 3.141074329178442 >500 0.001982519033064328 0.6684546822994042 >1k 0.0022798968880239777 2.93756597854932 >5k 5.947557099192986E-4 2.4946159845811806 >10k+ 4.95629758266082E-4 4.487337862547017 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36242 1.9227861458806954 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14184 0.752519140587489 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14027 0.7441896492541391 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10654 0.5652382207994295 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10081 0.5348382301369485 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9261 0.4913338804977959 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9003 0.4776459265869405 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8737 0.4635335399966788 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7592 0.4027866127566425 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7109 0.3771614897374831 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5656 0.3000739043403017 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4984 0.2644215592701668 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4459 0.23656816468412395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3952 0.20966974362674543 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3702 0.19640622239529643 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3652 0.19375351814900665 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3145 0.16685509709162813 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3005 0.1594275252020167 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2908 0.15428127896421448 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2874 0.15247744007673744 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2789 0.14796784285804476 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2438 0.1293458590490904 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2308 0.12244882800873695 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2233 0.11846977163930225 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.591622547773877E-4 0.0 0.0 0.0 0.0 3 1.591622547773877E-4 0.0 0.0 0.0 0.0 4 1.591622547773877E-4 0.0 0.0 0.0 0.0 5 1.591622547773877E-4 0.0 0.0 0.0 0.0 6 3.183245095547754E-4 0.0 0.0 0.0 0.0 7 3.183245095547754E-4 0.0 0.0 0.0 0.0 8 3.183245095547754E-4 0.0 0.0 0.0 0.0 9 3.183245095547754E-4 0.0 0.0 0.0 0.0 10-11 3.183245095547754E-4 0.0 0.0 0.0 0.0 12-13 4.2443267940636724E-4 0.0 0.0 0.0 0.0 14-15 5.30540849257959E-4 0.0 0.0 0.0 1.3263521231448975E-4 16-17 5.835949341837549E-4 0.0 0.0 0.0 1.591622547773877E-4 18-19 7.162301464982447E-4 0.0 0.0 0.0 1.591622547773877E-4 20-21 7.958112738869385E-4 0.0 0.0 1.0610816985159181E-4 1.591622547773877E-4 22-23 7.958112738869385E-4 0.0 0.0 3.183245095547754E-4 2.3874338216608158E-4 24-25 8.488653588127345E-4 0.0 0.0 5.30540849257959E-4 3.183245095547754E-4 26-27 8.488653588127345E-4 0.0 0.0 7.958112738869385E-4 3.183245095547754E-4 28-29 0.0010080276135901222 0.0 0.0 0.0015120414203851833 3.183245095547754E-4 30-31 0.001061081698515918 0.0 0.0 0.0029445017133816727 3.448515520176734E-4 32-33 0.001061081698515918 0.0 0.0 0.004376962006378162 4.2443267940636724E-4 34-35 0.0011406628259046118 0.0 0.0 0.0062603820212439164 4.2443267940636724E-4 36-37 0.0012202439532933058 0.0 0.0 0.009231410777088486 4.2443267940636724E-4 38-39 0.0012732980382191017 0.0 0.0 0.015863171392812975 4.509597218692652E-4 40-41 0.0013263521231448978 0.0 0.0 0.021964391159279506 4.7748676433216316E-4 42-43 0.0015385684628480812 0.0 0.0 0.028198246138060525 4.7748676433216316E-4 44-45 0.001697730717625469 0.0 0.0 0.03347712758817721 4.7748676433216316E-4 46-47 0.0019099470573286526 0.0 0.0 0.04109038877502892 4.7748676433216316E-4 48-49 0.0019630011422544485 0.0 0.0 0.04695286515932938 4.7748676433216316E-4 50-51 0.0019630011422544485 0.0 0.0 0.052258273651908965 4.7748676433216316E-4 52-53 0.0019630011422544485 0.0 0.0 0.06459334839715651 4.7748676433216316E-4 54-55 0.0019630011422544485 0.0 0.0 0.0841968327772381 5.040138067950611E-4 56-57 0.002122163397031836 0.0 0.0 0.11560485105330928 5.30540849257959E-4 58-59 0.002122163397031836 0.0 0.0 0.1567217668708011 5.30540849257959E-4 60-61 0.002148690439494734 0.0 0.0 0.20383379428490786 5.30540849257959E-4 62-63 0.002175217481957632 0.0 0.0 0.23144844548878463 5.83594934183755E-4 64-65 0.002175217481957632 0.0 0.0 0.2633870046141138 5.83594934183755E-4 66-67 0.002281325651809224 0.0 0.0 0.33864422408135525 5.83594934183755E-4 68-69 0.002281325651809224 0.0 0.0 0.44408921787137456 5.83594934183755E-4 70-71 0.002281325651809224 0.0 0.0 0.5425045454087261 5.83594934183755E-4 72-73 0.002307852694272122 0.0 0.0 0.6454559972072329 6.366490191095508E-4 74-75 0.002360906779197918 0.0 0.0 0.7453833661649696 6.366490191095508E-4 76-77 0.0023874338216608158 0.0 0.0 0.833930633906123 6.366490191095508E-4 78-79 0.0023874338216608158 0.0 0.0 0.9153686542672197 6.366490191095508E-4 80-81 0.0029710287558445706 0.0 0.0 0.9747361752991852 6.366490191095508E-4 82-83 0.0031036639681590603 0.0 0.0 1.0720904211380207 6.366490191095508E-4 84-85 0.003183245095547754 0.0 0.0 1.2795053661554197 6.366490191095508E-4 86-87 0.00323629918047355 0.0 0.0 1.582868623761121 6.366490191095508E-4 88-89 0.00323629918047355 0.0 0.0 1.93427235526713 6.897031040353468E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 430 0.0 25.413507 1 GTACATG 6145 0.0 22.808952 1 TACATGG 6265 0.0 22.061693 2 GAGTACT 6290 0.0 22.051273 12-13 ACATGGG 6105 0.0 21.783504 3 GTACTTT 6480 0.0 21.148146 14-15 GTAAGGT 665 0.0 20.711365 4 AGTACTT 6670 0.0 19.085802 12-13 CATGGGG 2950 0.0 18.353344 4 AGAGTAC 10330 0.0 17.703613 10-11 ACTTTTT 7985 0.0 17.636675 16-17 TATACTG 840 0.0 17.527288 5 GTAAGCG 305 0.0 17.130096 94-95 TACTTTT 7290 0.0 17.03906 14-15 CCGATCG 340 1.7538696E-8 16.762787 9 GCGTTCG 100 2.7609267E-4 16.626184 28-29 GGACCGT 200 2.76448E-4 16.622656 6 AAGGTGT 1450 0.0 16.377 6 GGTGTGC 1375 0.0 16.234074 8 GGTAAGG 740 0.0 16.045887 3 >>END_MODULE