FastQCFastQC Report
Thu 26 May 2016
SRR936564_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936564_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974260
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT287091.4541651049000637No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT194440.9848753456991481No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT142040.7194594430318195No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC122990.6229675929208919No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT92930.470708012115932No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT91620.4640726145492488No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC78850.399390151246543No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC73820.37391225066607237No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC70080.35496844387264087No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG58770.2976811564839484No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA56650.28694295584168245No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45220.22904784577512585No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT42290.21420684205727714No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA40120.20321538196590114No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC37770.19131218785772897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33990.17216577350500947No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC33230.16831622987853678No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG32370.16396016735384397No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC30550.15474152340623828No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC27840.1410148612644738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27140.13746922897693312No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT25460.12895971148683558No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG24140.12227366203033035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG66200.023.4746991
TACATGG68100.021.7713172
ACATGGG65800.021.5234383
GAGTACT47000.021.13663112-13
GTGTAGC11300.019.7664931
GTACTTT51050.019.36347814-15
TAGCCCT11900.019.1697084
GTGTACG3003.6980055E-919.0094781
AATACGG1559.590756E-418.3976695
AGTACTT52750.018.15682212-13
CATGGGG33450.018.0438354
AGAGTAC86050.018.00935410-11
GCACCGT4559.094947E-1216.7129026
ACTTTTT62650.015.96563516-17
GTAAGGT6850.015.9572684
GTAGCCC10850.015.7686323
GGACCAT8850.015.5739216
TACTTTT61150.015.46579814-15
CGCGGAA1552.0725201E-615.317800582-83
CATGGGA31200.015.0800194
Produced by FastQC (version 0.11.5)