FastQCFastQC Report
Thu 26 May 2016
SRR936564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936564_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974260
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT370811.8782227264899254No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT151320.7664643967866441No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT144460.7317172003687458No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC109850.5564110096947716No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT102210.5177129658707567No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC96950.49107007182437973No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT93310.47263278392916835No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC90470.4582476472197177No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG76960.38981694407018325No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA72610.36778337199760924No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC60850.30821674956692635No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA51800.262376789278008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46230.23416368664714882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41000.207672748270238No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC38310.19404738990811748No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT37870.19181870675594906No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA33540.16988643846301907No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT31150.15778063679555884No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG30350.1537284856097981No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC30320.15357652994033208No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG29990.15190501757620578No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC25690.13012470495274178No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC24120.12217235825068634No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.11325762564201271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT68450.022.44327712-13
GTACATG69150.021.9890921
TACATGG68950.021.5617282
ACATGGG67100.021.020953
GTACTTT71650.020.9769814-15
AGTACTT72150.020.04178612-13
TAAGGTG7250.018.995755
AGAGTAC112050.018.6044210-11
CAATTCG2051.8189894E-1218.54042278-79
AATTCGC2051.8189894E-1218.54042278-79
GTAAGCG3150.018.10655494-95
ACTTTTT85300.017.89853116-17
TACTTTT80800.017.66113314-15
ACCGTAT1350.00941895917.5873228
TAGACTG7100.017.3904745
CATGGGG32750.017.1106764
GTGTAGC11150.017.0464761
GTATAGG5300.017.0344161
CCTACAC9500.016.9970593
GTTATAT4559.094947E-1216.7092951
Produced by FastQC (version 0.11.5)