##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936563_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 546201 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.736007440484364 31.0 31.0 34.0 30.0 34.0 2 31.759850311515358 33.0 31.0 34.0 30.0 34.0 3 31.748843374508652 33.0 31.0 34.0 30.0 34.0 4 35.397040649870654 37.0 35.0 37.0 33.0 37.0 5 35.447315182506074 37.0 35.0 37.0 33.0 37.0 6 35.49048244144555 37.0 35.0 37.0 33.0 37.0 7 35.44384027125545 37.0 35.0 37.0 33.0 37.0 8 35.42706988819134 37.0 35.0 37.0 33.0 37.0 9 36.92948749636123 39.0 37.0 39.0 33.0 39.0 10-11 36.81010012797486 39.0 37.0 39.0 33.0 39.0 12-13 36.7485751582293 39.0 37.0 39.0 32.0 39.0 14-15 37.73699608752089 40.0 37.0 41.0 32.5 41.0 16-17 37.71603402410468 40.0 37.0 41.0 32.5 41.0 18-19 37.830459849029936 40.0 37.5 41.0 33.0 41.0 20-21 37.75445211561312 40.0 37.0 41.0 32.5 41.0 22-23 37.839711022132875 40.0 37.5 41.0 33.0 41.0 24-25 37.842357483783445 40.0 37.0 41.0 33.0 41.0 26-27 37.722652466765894 40.0 37.0 41.0 33.0 41.0 28-29 37.60238172394412 40.0 37.0 41.0 32.5 41.0 30-31 37.34619856060315 39.0 36.5 41.0 32.0 41.0 32-33 37.02892616454382 39.0 35.5 41.0 31.5 41.0 34-35 36.74578314576502 39.0 35.0 41.0 31.0 41.0 36-37 36.47251011990092 39.0 35.0 40.0 30.5 41.0 38-39 36.203444336425605 38.0 35.0 40.0 30.0 41.0 40-41 35.99300257597478 38.0 35.0 40.0 30.0 41.0 42-43 35.622908965747044 38.0 35.0 40.0 29.0 41.0 44-45 35.11840512924729 38.0 35.0 40.0 26.5 41.0 46-47 34.51513728462599 38.0 34.0 40.0 23.5 41.0 48-49 34.09052619823105 38.0 34.0 40.0 20.0 41.0 50-51 33.05039811351499 37.5 32.5 39.5 16.5 40.5 52-53 33.1521829875815 37.5 33.0 39.5 14.5 40.5 54-55 33.56713920333357 38.0 34.0 40.0 13.5 41.0 56-57 33.351543662497875 38.0 33.0 40.0 9.0 41.0 58-59 33.10249065820092 38.0 33.0 40.0 8.0 41.0 60-61 32.70064866230564 37.0 32.5 40.0 6.5 41.0 62-63 32.40119571366584 36.5 32.0 40.0 2.0 41.0 64-65 32.04819288137517 36.0 31.5 39.0 2.0 41.0 66-67 31.495311249887862 35.5 31.0 39.0 2.0 41.0 68-69 31.067783654735162 35.0 30.5 39.0 2.0 40.0 70-71 30.548880357231127 35.0 30.0 38.0 2.0 40.0 72-73 30.1715970860544 35.0 29.5 37.0 2.0 39.5 74-75 29.762647816463172 35.0 29.0 37.0 2.0 39.0 76-77 29.320183412333556 34.0 29.0 36.0 2.0 39.0 78-79 28.782907757400665 34.0 28.5 36.0 2.0 38.5 80-81 28.230525026501233 34.0 27.0 35.0 2.0 37.0 82-83 27.77516060937274 34.0 26.0 35.0 2.0 37.0 84-85 27.394059146724373 34.0 26.0 35.0 2.0 36.0 86-87 27.1284810902946 34.0 26.0 35.0 2.0 36.0 88-89 26.65249880538483 33.0 25.0 35.0 2.0 36.0 90-91 26.163919509484604 33.0 23.5 35.0 2.0 35.0 92-93 25.64765443490583 33.0 20.5 35.0 2.0 35.0 94-95 25.138725487503685 32.0 18.5 35.0 2.0 35.0 96-97 24.549277646873588 32.0 6.0 35.0 2.0 35.0 98-99 23.867294274452078 32.0 2.0 35.0 2.0 35.0 100-101 21.15663189924588 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.368275591806551 1101 2 0.42897501183981745 1101 3 0.42421423183521156 1101 4 0.38418729020735043 1101 5 0.22428339459565905 1101 6 0.4362979724144651 1101 7 0.41673708836483314 1101 8 0.451299768645562 1101 9 0.359354238673518 1101 10-11 0.7988315153544292 1101 12-13 0.7115665116071668 1101 14-15 0.617930413757179 1101 16-17 0.6686610088160378 1101 18-19 0.7146673581685761 1101 20-21 0.7759430537153875 1101 22-23 0.6928198265963772 1101 24-25 0.7374923494866934 1101 26-27 0.671203319749857 1101 28-29 0.7292069639375711 1101 30-31 0.5636588138367955 1101 32-33 0.35360967455053327 1101 34-35 0.34121591366802306 1101 36-37 0.37839909918539405 1101 38-39 0.27865156480145004 1101 40-41 0.47773892710035426 1101 42-43 0.5727375752338517 1101 44-45 0.39155886080952484 1101 46-47 0.688253387094619 1101 48-49 0.6947216878510503 1101 50-51 0.6286593248612391 1101 52-53 0.5596729395649405 1101 54-55 0.5495980043475086 1101 56-57 0.39376923855554935 1101 58-59 0.3471011365827579 1101 60-61 0.42034367103525483 1101 62-63 0.4643831950574011 1101 64-65 0.5354992969142245 1101 66-67 0.5437976095752894 1101 68-69 0.5135458287311785 1101 70-71 0.6124684621494616 1101 72-73 0.3758224605476457 1101 74-75 0.2613980441821937 1101 76-77 0.5217400493655084 1101 78-79 0.48753777248746033 1101 80-81 0.3442457969720003 1101 82-83 0.34754530309406917 1101 84-85 0.36129146716331917 1101 86-87 0.6457844987777577 1101 88-89 0.6334526129286253 1101 90-91 0.5819736156114281 1101 92-93 0.4282584362749695 1101 94-95 0.36365725663642934 1101 96-97 0.5241697532003613 1101 98-99 0.5939791848799665 1101 100-101 0.9655691145385852 1102 1 0.15842603542234457 1102 2 0.13417410317752498 1102 3 0.11356888158590195 1102 4 0.19954025407516696 1102 5 0.15267623387958906 1102 6 0.3207721341163463 1102 7 0.2880099299347023 1102 8 0.2797969040733861 1102 9 0.16679212531932563 1102 10-11 0.2237668660323564 1102 12-13 0.2543493613207133 1102 14-15 0.3217222215093827 1102 16-17 0.3963980682362589 1102 18-19 0.2892105267711571 1102 20-21 0.3597371474104776 1102 22-23 0.11409052509479523 1102 24-25 0.09110628230855156 1102 26-27 -0.24218801938405932 1102 28-29 -0.15316698774331883 1102 30-31 0.2926031368405191 1102 32-33 0.12855859801430825 1102 34-35 0.07858250747026574 1102 36-37 -0.3359437636723257 1102 38-39 -0.21324762511754614 1102 40-41 -0.21186574765282984 1102 42-43 -0.3536264897440802 1102 44-45 -0.2240384132035942 1102 46-47 -0.1720540722227355 1102 48-49 0.1667546054285225 1102 50-51 0.24450662387685895 1102 52-53 0.39188473217276965 1102 54-55 0.4523311348034156 1102 56-57 0.4301443046335862 1102 58-59 0.44013186822736117 1102 60-61 0.48226414879732005 1102 62-63 0.7136652661216587 1102 64-65 0.6394525493823267 1102 66-67 0.5947669922278429 1102 68-69 0.562557872792727 1102 70-71 0.38618154774373536 1102 72-73 0.12874060950539956 1102 74-75 0.5538344306779877 1102 76-77 0.5153036914440001 1102 78-79 0.35012403111332446 1102 80-81 0.42545391778407904 1102 82-83 0.39221405568361334 1102 84-85 -0.08008124153612073 1102 86-87 -0.03031168226891623 1102 88-89 0.012776259579002414 1102 90-91 -0.010592783885662982 1102 92-93 -0.17820935336113664 1102 94-95 -0.16068803503559792 1102 96-97 -0.1341889398117999 1102 98-99 -0.13732966029026628 1102 100-101 -0.38435873538926657 1103 1 0.29143630873112514 1103 2 0.09091636588993879 1103 3 0.22802474081788304 1103 4 0.19990854250315238 1103 5 0.28120458778107604 1103 6 0.3699655609217132 1103 7 0.27721757138115066 1103 8 0.3296041449254048 1103 9 0.2500274637785438 1103 10-11 0.4793504201233745 1103 12-13 0.24456171944351723 1103 14-15 0.3075280408972887 1103 16-17 0.4859750749774463 1103 18-19 0.5508999153262124 1103 20-21 0.47600321964669945 1103 22-23 0.24983496307804387 1103 24-25 0.5096154679819094 1103 26-27 0.17885072628155285 1103 28-29 0.22590733759966497 1103 30-31 0.18304702131425898 1103 32-33 0.17924563899985912 1103 34-35 0.18421344369821213 1103 36-37 0.30596142897913836 1103 38-39 0.4075122120601975 1103 40-41 0.19238141480328608 1103 42-43 0.10313192596956355 1103 44-45 0.5394234980195094 1103 46-47 0.4926623887623762 1103 48-49 0.5821839522011558 1103 50-51 0.44219243723431134 1103 52-53 0.8119909112341688 1103 54-55 0.9914935478464244 1103 56-57 1.0062098642812387 1103 58-59 0.8786470843545118 1103 60-61 0.7222309813014647 1103 62-63 0.7170315515774917 1103 64-65 0.31529304387953516 1103 66-67 0.6369112492974729 1103 68-69 0.5196458097314078 1103 70-71 0.7547155013523188 1103 72-73 0.7545617204011386 1103 74-75 0.9011487250072072 1103 76-77 1.103132943452355 1103 78-79 0.7519913180552358 1103 80-81 0.857812686710016 1103 82-83 0.7513674230459344 1103 84-85 0.6291765819008077 1103 86-87 0.5015646503536821 1103 88-89 0.5751623809203501 1103 90-91 0.3390636956215616 1103 92-93 -0.12354759720684427 1103 94-95 0.45382382126730647 1103 96-97 0.5740124773448407 1103 98-99 0.6548544408490606 1103 100-101 0.4564006710417807 1104 1 0.17865039310800057 1104 2 0.2082630689073568 1104 3 0.09682470385101283 1104 4 0.2692399042701723 1104 5 0.4974977350478369 1104 6 0.5044510283215544 1104 7 0.4430879100900853 1104 8 0.26952836284509374 1104 9 0.2883482437794669 1104 10-11 0.42950290845570294 1104 12-13 0.490100280508166 1104 14-15 0.43691439088112105 1104 16-17 0.29273539104417523 1104 18-19 0.2732325247250387 1104 20-21 0.10790100625545307 1104 22-23 0.40858455875292066 1104 24-25 0.3629702112932236 1104 26-27 0.27571547375163163 1104 28-29 0.3171554314955074 1104 30-31 0.3912421139624698 1104 32-33 0.4141377419525867 1104 34-35 0.387124584588193 1104 36-37 0.0049563922433080165 1104 38-39 -0.06970695526108273 1104 40-41 -0.3745563422319691 1104 42-43 -0.32611117739904927 1104 44-45 0.024946348165073573 1104 46-47 0.2891736405529599 1104 48-49 0.4319228736927627 1104 50-51 0.4435324862154104 1104 52-53 0.6765558574011692 1104 54-55 0.3128927438170379 1104 56-57 0.4759986910452483 1104 58-59 0.3411511276057482 1104 60-61 0.594665249588509 1104 62-63 0.5936292134230641 1104 64-65 0.4903219242455066 1104 66-67 0.6565937963147057 1104 68-69 0.7630356715677422 1104 70-71 0.5961784651709934 1104 72-73 0.4844147770990723 1104 74-75 0.5229707295356469 1104 76-77 0.44936232287105327 1104 78-79 0.5412870992113774 1104 80-81 0.6342624810818087 1104 82-83 0.19681356693947194 1104 84-85 0.2295855278829677 1104 86-87 0.14947602228658852 1104 88-89 0.06790070170034923 1104 90-91 0.38028637479535377 1104 92-93 -0.029582438480350248 1104 94-95 -0.20911458268815153 1104 96-97 0.06969197459542897 1104 98-99 0.5779333189046021 1104 100-101 0.7877716797752043 1105 1 0.33651646351277265 1105 2 0.2733836590537493 1105 3 0.25640598275633764 1105 4 0.3538011166693593 1105 5 0.46857329977665785 1105 6 0.4367934584710014 1105 7 0.5224174976756046 1105 8 0.5099155508587003 1105 9 0.5935598020870003 1105 10-11 0.5841965597953802 1105 12-13 0.32915495695963415 1105 14-15 0.44293434272150733 1105 16-17 0.4412012588727876 1105 18-19 0.3372805123411382 1105 20-21 0.33933471034136886 1105 22-23 0.19596081529842024 1105 24-25 0.248483627185891 1105 26-27 0.3246610266316594 1105 28-29 0.35468935344636066 1105 30-31 0.2442525676566234 1105 32-33 0.25488134933705453 1105 34-35 0.23486758042200506 1105 36-37 0.515266754204859 1105 38-39 0.31516146579154736 1105 40-41 0.0820856712985929 1105 42-43 0.32127731015972216 1105 44-45 0.37277295444111047 1105 46-47 0.6581727996708011 1105 48-49 0.4808779820519007 1105 50-51 0.568159822530049 1105 52-53 0.7645498756712428 1105 54-55 0.7509923501312983 1105 56-57 0.5809680179572965 1105 58-59 0.7289626362247859 1105 60-61 1.0532518031183074 1105 62-63 0.7762654745551956 1105 64-65 0.739129878226322 1105 66-67 0.9543449143057607 1105 68-69 1.0146983249331782 1105 70-71 1.0135164042452622 1105 72-73 0.8817268208249445 1105 74-75 1.2069829796598697 1105 76-77 0.9157365918769003 1105 78-79 0.9226749369975629 1105 80-81 1.097261277091441 1105 82-83 0.9913803215165444 1105 84-85 0.8107617118068333 1105 86-87 0.6661168891596532 1105 88-89 0.9164559132586554 1105 90-91 0.6700730009467861 1105 92-93 0.5177482384297853 1105 94-95 0.35192820674730996 1105 96-97 0.7242566269671009 1105 98-99 0.8294813065207016 1105 100-101 0.49070138967085697 1106 1 0.3239785157289212 1106 2 0.39258017797704525 1106 3 0.30607710196297333 1106 4 0.19330222980435252 1106 5 0.17680037931521042 1106 6 0.33421806887375993 1106 7 0.3048906812416803 1106 8 0.5057969513849088 1106 9 0.29656526656787463 1106 10-11 0.4461475818556906 1106 12-13 0.49791381144253677 1106 14-15 0.4627886232315248 1106 16-17 0.34813558390820987 1106 18-19 0.16740346316080945 1106 20-21 0.13012604812724504 1106 22-23 0.5029179090041538 1106 24-25 0.3725956490438165 1106 26-27 0.4652221478621783 1106 28-29 0.4363428648812899 1106 30-31 0.4272848485714107 1106 32-33 0.5355583378009356 1106 34-35 0.4747076937593846 1106 36-37 0.5799398972933005 1106 38-39 0.4276614657915445 1106 40-41 0.669259320357483 1106 42-43 0.49497190639529975 1106 44-45 0.27486514225734027 1106 46-47 0.3317864406341684 1106 48-49 0.6400992935273067 1106 50-51 0.6957402718318093 1106 52-53 0.6546505637910585 1106 54-55 0.726837320785009 1106 56-57 0.9131097908777619 1106 58-59 0.9199487726344202 1106 60-61 0.8627741670122546 1106 62-63 0.8981056890866697 1106 64-65 0.8172137681271465 1106 66-67 0.922218320498839 1106 68-69 1.1656697881997218 1106 70-71 0.9964576104788172 1106 72-73 0.856139895101407 1106 74-75 1.2257266550292272 1106 76-77 1.1177402348641508 1106 78-79 0.9347170337391155 1106 80-81 1.214724847218946 1106 82-83 1.3970977876165271 1106 84-85 1.3749314142962064 1106 86-87 1.1911168891596517 1106 88-89 1.0571662957723191 1106 90-91 1.0875466904833218 1106 92-93 1.0875240935198498 1106 94-95 1.236781981286878 1106 96-97 0.8215901626886151 1106 98-99 0.5450550770125027 1106 100-101 0.6296206367868287 1107 1 0.19732299372719808 1107 2 0.21851848686401354 1107 3 0.2222117007550395 1107 4 0.18516482687078906 1107 5 0.21150702297353519 1107 6 0.36958260726892433 1107 7 0.4205453404306425 1107 8 0.37866148978007885 1107 9 0.47294576699928115 1107 10-11 0.23281173198511596 1107 12-13 0.23937466131311425 1107 14-15 0.14149062927121037 1107 16-17 0.23120288330424188 1107 18-19 0.27889235444639837 1107 20-21 0.5430790248313002 1107 22-23 0.5941495742327945 1107 24-25 0.4699748552560692 1107 26-27 0.5765088174048856 1107 28-29 0.34245535409613126 1107 30-31 0.3324185845507017 1107 32-33 0.2673691661011901 1107 34-35 0.4604604979008826 1107 36-37 0.6316978783114209 1107 38-39 0.5372009394757526 1107 40-41 0.7402849890373844 1107 42-43 0.7821995811148881 1107 44-45 0.7701088868647616 1107 46-47 0.8605261947325289 1107 48-49 1.010483245209791 1107 50-51 0.6435131363702027 1107 52-53 0.8782296190110728 1107 54-55 0.824599400163784 1107 56-57 0.9490540316025218 1107 58-59 0.7280996570175162 1107 60-61 0.8148664879786054 1107 62-63 0.8829093991815782 1107 64-65 0.9264674350814204 1107 66-67 0.9634823412063476 1107 68-69 1.0607814146017986 1107 70-71 1.038999672601335 1107 72-73 0.8459182600712083 1107 74-75 0.9217226645201677 1107 76-77 0.7651517965552621 1107 78-79 0.8017360156200475 1107 80-81 0.8169250197814968 1107 82-83 0.7343038920168681 1107 84-85 0.0742546455300257 1107 86-87 0.25739978389649565 1107 88-89 0.4677168781709362 1107 90-91 0.5836532478603687 1107 92-93 0.7340108277735169 1107 94-95 0.7759860365068967 1107 96-97 0.7539025009112201 1107 98-99 0.6913234117838591 1107 100-101 0.8116687385987333 1108 1 0.2850779423905969 1108 2 0.31436415331484824 1108 3 0.42242444703197535 1108 4 0.5756843209046423 1108 5 0.35181550507510195 1108 6 0.286386094457896 1108 7 0.29262296969712764 1108 8 0.20682262196026358 1108 9 0.4053641371778056 1108 10-11 0.40569409716872684 1108 12-13 0.16982599560225964 1108 14-15 0.5235826535982895 1108 16-17 0.4229173408012272 1108 18-19 0.41186311339562565 1108 20-21 0.433809530797447 1108 22-23 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-2.9268837036407866 2316 92-93 -3.0391313413039107 2316 94-95 -3.155269281348943 2316 96-97 -3.357134329038942 2316 98-99 -4.709779663456203 2316 100-101 -5.274780605025917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 820.0 3 294.0 4 268.0 5 309.0 6 387.0 7 464.0 8 566.0 9 844.0 10 1096.0 11 1665.0 12 2524.0 13 3460.0 14 4238.0 15 5936.0 16 8064.0 17 11663.0 18 8057.0 19 4494.0 20 4218.0 21 4381.0 22 4779.0 23 5061.0 24 5901.0 25 6503.0 26 7435.0 27 8835.0 28 10086.0 29 11980.0 30 14728.0 31 18433.0 32 23026.0 33 29656.0 34 39487.0 35 54221.0 36 72198.0 37 89718.0 38 69850.0 39 10555.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.27258078987218 19.21653644951194 14.981900212217036 18.52898254839885 2 14.863825626490007 22.70763067210119 39.06283759782326 23.36570610358554 3 21.53145267907171 28.68937709888999 30.325641523322627 19.453528698715672 4 11.199598601698252 16.772845551382463 40.18780752658439 31.839748320334888 5 10.998805930787938 41.231283148240394 32.99788290796144 14.772028013010226 6 26.82170883432616 37.94613090658636 17.879307439239735 17.35285281984774 7 25.351581452831052 36.79589875040051 20.903556552387055 16.94896324438138 8 26.473717656409384 29.886951951374506 20.20031095996528 23.439019432250834 9 27.331285703034997 17.60302712285965 22.445544572589228 32.62014260151613 10-11 24.751158695228128 26.966164542506554 25.826654981267644 22.456021780997673 12-13 28.67116090053165 25.785437876465338 26.326613959229572 19.216787263773437 14-15 24.133807401655698 25.954508120541302 29.67319136311735 20.23849311468565 16-17 19.3313676653725 30.067577466852246 29.055746831733938 21.545308036041316 18-19 18.921552020144215 28.693876616687653 32.92287970699325 19.46169165617489 20-21 22.05835614706914 25.147987796444998 31.780625436773512 21.01303061971235 22-23 20.418856316261696 25.75055389742369 32.75895848973688 21.07163129657774 24-25 21.62095854319889 25.86861912582413 32.45880959312172 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28.285533026290853 31.65741602791029 20.8581992883279 52-53 20.704425592140254 29.588291675477933 28.296582325644415 21.410700406737394 54-55 19.609945082157058 27.766054932490142 29.638974000305762 22.98502598504704 56-57 21.59234047850955 27.53031878666266 28.350720254267696 22.52662048056009 58-59 22.862491817986243 27.123547263431092 28.446584610032637 21.56737630855003 60-61 21.23114301329132 27.789012300810988 30.968621351615344 20.011223334282345 62-63 20.52078470143447 28.339878614780435 30.307582456814874 20.83175422697022 64-65 19.918325947394294 30.021576523424304 29.274225071425892 20.78587245775551 66-67 20.781909205437714 28.28751245165827 27.95790973280206 22.972668610101955 68-69 20.218473142346387 28.2819050828707 29.16226319712952 22.33735857765339 70-71 22.022902895788434 28.221137597806244 28.17964750825909 21.576311998146227 72-73 21.02581098798075 27.19400722180311 30.393280646886055 21.386901143330086 74-75 19.620163911908794 30.65097751934435 28.582940341559453 21.145918227187398 76-77 20.106912601054656 28.326620010676614 28.788387950997475 22.778079437271256 78-79 20.350818702148494 28.35593690722292 29.238891397513623 22.05435299311497 80-81 21.462187445317532 28.552516699052433 28.07822788034033 21.90706797528971 82-83 20.672952317280348 27.734647244952626 29.75167253561436 21.84072790215266 84-85 20.107376553128415 28.734580871628808 29.059603691893017 22.098438883349765 86-87 19.962973882120707 29.612928743950295 28.287166659647205 22.136930714281792 88-89 19.63051607256 29.00303248938812 27.155979094867845 24.21047234318404 90-91 20.005311428270534 27.448087656881732 28.4190716905452 24.12752922430253 92-93 21.418571539753973 28.293053641653426 28.787855203702772 21.50051961488983 94-95 20.150605167267866 27.658616080420412 29.156153296834088 23.034625455477634 96-97 18.223501998251244 27.96901680545319 30.829194153112283 22.978287043183286 98-99 19.374228386581528 29.11524103646228 28.197364516589918 23.31316606036627 100-101 18.911783727137987 27.620097136649026 29.19962112676314 24.268498009449846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 250.0 1 218.5 2 260.5 3 337.0 4 308.5 5 337.5 6 506.0 7 923.0 8 1457.5 9 1783.5 10 1647.0 11 1357.5 12 1371.0 13 1471.5 14 1565.5 15 1647.5 16 1689.5 17 1734.5 18 1831.0 19 1912.5 20 2062.0 21 2307.0 22 2530.0 23 2836.0 24 3115.5 25 3522.5 26 4199.0 27 4882.0 28 5507.0 29 6498.0 30 7726.5 31 8864.0 32 10483.5 33 11984.5 34 13029.5 35 14175.0 36 16169.5 37 17934.0 38 17370.0 39 16260.5 40 15364.0 41 14472.5 42 13789.5 43 14039.0 44 14722.5 45 15115.5 46 16067.0 47 23554.5 48 40326.0 49 39236.5 50 22442.0 51 14565.5 52 11779.5 53 10839.5 54 10242.5 55 9675.5 56 9222.5 57 8371.0 58 8118.5 59 7824.0 60 7036.5 61 6455.5 62 5512.0 63 3803.5 64 2380.5 65 1821.5 66 1377.0 67 948.0 68 676.5 69 488.0 70 351.0 71 300.5 72 265.5 73 276.0 74 243.0 75 184.5 76 137.0 77 83.5 78 50.5 79 27.5 80 20.5 81 20.0 82 13.0 83 6.0 84 5.0 85 2.0 86 0.5 87 2.0 88 3.0 89 1.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011351132641646573 2 0.01153421542618926 3 0.014463539978872248 4 0.020139106299695536 5 0.030940990587714046 6 0.052178593594665694 7 0.004760152398109853 8 0.027462417681403 9 0.03734888804670808 10-11 0.04036975399166241 12-13 0.030757907803171364 14-15 0.027004710720046284 16-17 0.029476328311372552 18-19 0.025356965659162102 20-21 0.05391788004782122 22-23 0.012998877702530753 24-25 0.00329549012176836 26-27 0.0011900380995274633 28-29 0.0 30-31 0.0021054520222408965 32-33 0.0015562036686128367 34-35 0.0 36-37 9.154139227134333E-5 38-39 0.0 40-41 7.323311381707467E-4 42-43 0.0034785729063110467 44-45 0.0012815794917988067 46-47 0.00109849670725612 48-49 9.154139227134333E-4 50-51 0.004119362652210449 52-53 0.00466861100583851 54-55 0.003936279867667764 56-57 9.154139227134333E-4 58-59 0.006133273282180003 60-61 0.0032039487294970165 62-63 0.00109849670725612 64-65 0.0010069553149847767 66-67 0.016294367824299112 68-69 0.04210904044481793 70-71 0.05263630055602241 72-73 0.014097374409786874 74-75 0.032222570079512854 76-77 0.027004710720046284 78-79 0.056114873462333466 80-81 0.07991563545288273 82-83 0.04549607195885764 84-85 0.01565357807839971 86-87 0.01830827845426867 88-89 0.021054520222408966 90-91 0.03817276057715017 92-93 0.0226107238910218 94-95 0.012998877702530753 96-97 0.018033654277454635 98-99 0.04888310347289734 100-101 0.06810679584987944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 546201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.56663538736258 #Duplication Level Percentage of deduplicated Percentage of total 1 79.79681084344664 38.75462617308013 2 11.287912062879153 10.964318188849271 3 3.6187658244682153 5.272538410475891 4 1.6180270691648506 3.143285228600487 5 0.8298247982382729 2.015089920571496 6 0.5316234642720922 1.549149777160157 7 0.3615342705232906 1.2290952167578533 8 0.27452919037101275 1.066636727354946 9 0.19694724304487365 0.8608558449155992 >10 1.2931439174626758 11.97598705764312 >50 0.11612262972338958 3.8970963446043823 >100 0.061856172000608514 5.313284013272434 >500 0.0034153714601562984 1.1441557859931066 >1k 0.00796920007369803 7.8398999395255755 >5k 0.001517942871180577 4.973981371195548 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9480 1.7356247974646695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6872 1.2581448953773429 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5558 1.0175741164882524 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5078 0.9296943799077629 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4431 0.8112398183086447 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4161 0.7618074664821192 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3115 0.570302873850469 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3036 0.5558393338715968 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2699 0.49414043548071135 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2638 0.48297238562360745 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2480 0.4540453056658629 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2465 0.4512990638977227 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2005 0.3670809830080867 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1947 0.3564621815046109 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1561 0.2857922266711339 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1504 0.27535650795220074 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1395 0.2554004844370479 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1367 0.2502741664698527 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1256 0.22995197738561443 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1189 0.21768543082125447 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 0.2019403113505834 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1100 0.20139106299695533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1035 0.1894906820016807 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1028 0.18820910250988188 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1023 0.18729368858716847 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 855 0.1565357807839971 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 841 0.15397262180039947 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 838 0.15342337344677143 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 743 0.1360305089152162 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 678 0.12413012791994155 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 0.12284854842814276 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 551 0.10087861428302036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8308278454268667E-4 0.0 18-19 0.0 0.0 0.0 1.8308278454268667E-4 0.0 20-21 0.0 0.0 0.0 2.7462417681403E-4 0.0 22-23 0.0 0.0 0.0 5.4924835362806E-4 0.0 24-25 0.0 0.0 0.0 0.0014646622763414933 0.0 26-27 0.0 0.0 0.0 0.0023800761990549267 0.0 28-29 0.0 0.0 0.0 0.00576710771309463 0.0 30-31 0.0 0.0 0.0 0.013273501879344785 0.0 32-33 0.0 0.0 0.0 0.02224455832193643 0.0 34-35 0.0 0.0 0.0 0.030940990587714046 0.0 36-37 0.0 0.0 0.0 0.0371658052621654 0.0 38-39 0.0 0.0 0.0 0.04274983019071733 0.0 40-41 0.0 0.0 0.0 0.05098855549513824 0.0 42-43 0.0 0.0 0.0 0.06014269472227257 0.0 44-45 0.0 0.0 0.0 0.07057841344120572 0.0 46-47 0.0 0.0 0.0 0.08467578785099258 0.0 48-49 0.0 0.0 0.0 0.09913932782986483 0.0 50-51 0.0 0.0 0.0 0.1216585103286153 0.0 52-53 0.0 0.0 0.0 0.1666968753261162 0.0 54-55 0.0 0.0 0.0 0.2150307304453855 0.0 56-57 0.0 0.0 0.0 0.28670764059384735 0.0 58-59 0.0 0.0 0.0 0.3787982812188187 0.0 60-61 0.0 0.0 0.0 0.5175750319021752 0.0 62-63 0.0 0.0 0.0 0.6104895450575887 0.0 64-65 0.0 0.0 0.0 0.6923275497481696 0.0 66-67 0.0 0.0 0.0 0.882001314534393 0.0 68-69 0.0 0.0 0.0 1.120375100008971 0.0 70-71 0.0 0.0 0.0 1.2990638977226332 0.0 72-73 0.0 0.0 0.0 1.4420515524504716 0.0 74-75 0.0 0.0 0.0 1.7760860928486033 0.0 76-77 0.0 0.0 0.0 1.9920322372167023 0.0 78-79 0.0 0.0 0.0 2.140786999657635 0.0 80-81 0.0 0.0 0.0 2.308216206121922 0.0 82-83 0.0 0.0 0.0 2.594008432793056 0.0 84-85 0.0 0.0 0.0 2.945161213545929 0.0 86-87 0.0 0.0 0.0 3.3680824458395353 0.0 88-89 0.0 0.0 0.0 4.044298710547948 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGACGT 25 0.0050099096 57.0286 7 GTATATG 150 9.458745E-10 31.68546 1 GTACATG 6055 0.0 28.65035 1 TACATGG 6050 0.0 28.44096 2 GAGCGGT 85 6.384079E-4 27.955196 7 AGGTAAG 140 5.762122E-7 27.161453 2 GCGGTCG 70 0.0083635375 27.158964 9 ACATGGG 6160 0.0 26.384985 3 GTGTAAG 145 7.834169E-7 26.222448 1 GGTAAGG 145 7.8404264E-7 26.220043 3 TACCTGG 310 0.0 26.066235 2 ATGGGAG 1580 0.0 25.867403 5 CATGGGA 4695 0.0 25.811665 4 GAGAGTC 115 1.276194E-4 24.795042 7 TAAGGCG 155 1.4022298E-6 24.528429 5 GAGTACT 4710 0.0 24.218184 12-13 TATATGG 120 1.7012923E-4 23.766273 2 CGAACAT 70 1.7724804E-5 23.761915 64-65 CTTGCAC 100 0.0016477185 23.761915 3 GTAAGCG 90 1.9457184E-7 23.756472 94-95 >>END_MODULE