##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936563_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 546201 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07091162410907 31.0 31.0 34.0 31.0 34.0 2 32.14512239999561 33.0 31.0 34.0 30.0 34.0 3 32.22875644680255 34.0 31.0 34.0 30.0 34.0 4 35.95033513303711 37.0 35.0 37.0 35.0 37.0 5 35.87145391531689 37.0 35.0 37.0 35.0 37.0 6 35.989853552080646 37.0 35.0 37.0 35.0 37.0 7 35.931047361685536 37.0 35.0 37.0 35.0 37.0 8 35.92183463596734 37.0 35.0 37.0 35.0 37.0 9 37.51188664978643 39.0 37.0 39.0 35.0 39.0 10-11 37.42010267282557 39.0 37.0 39.0 34.0 39.0 12-13 37.32234562001901 39.0 37.0 39.0 34.0 39.0 14-15 38.25588016133255 40.0 37.5 41.0 33.5 41.0 16-17 38.31127735027948 40.0 37.5 41.0 33.5 41.0 18-19 38.624205191861606 40.0 38.0 41.0 34.0 41.0 20-21 38.69566423349646 40.0 38.0 41.0 34.5 41.0 22-23 38.5915313227182 40.0 38.0 41.0 34.0 41.0 24-25 38.43215318170417 40.0 38.0 41.0 34.0 41.0 26-27 38.18924260482862 40.0 37.5 41.0 34.0 41.0 28-29 38.16193397668624 40.0 37.0 41.0 34.0 41.0 30-31 37.943950120926175 40.0 37.0 41.0 33.5 41.0 32-33 37.579415819451086 40.0 35.5 41.0 33.0 41.0 34-35 37.2587115365955 39.0 35.0 41.0 33.0 41.0 36-37 36.983492340731715 39.0 35.0 41.0 32.5 41.0 38-39 36.720127755167056 39.0 35.0 41.0 31.5 41.0 40-41 36.470141944082854 39.0 35.0 41.0 31.0 41.0 42-43 36.02016290706169 39.0 35.0 40.0 30.0 41.0 44-45 35.420304979302486 39.0 35.0 40.0 28.0 41.0 46-47 34.60824037304948 38.0 34.5 40.0 23.5 41.0 48-49 33.83784540855839 38.0 34.0 40.0 15.5 41.0 50-51 33.408342350160474 38.0 33.0 40.0 8.5 41.0 52-53 33.19210418875103 38.0 33.0 40.0 6.0 41.0 54-55 32.89733266691199 38.0 33.0 40.0 2.0 41.0 56-57 32.485694826629754 37.5 32.5 40.0 2.0 41.0 58-59 32.137837535998656 37.0 32.0 40.0 2.0 41.0 60-61 31.650368637186677 36.0 31.0 39.0 2.0 40.5 62-63 31.41374329230448 36.0 31.0 39.0 2.0 40.0 64-65 31.13581538664338 35.5 31.0 39.0 2.0 40.0 66-67 30.64472236411138 35.0 30.0 38.5 2.0 40.0 68-69 30.15696510991375 35.0 29.5 38.0 2.0 40.0 70-71 29.599093557133727 34.5 29.0 37.0 2.0 39.0 72-73 29.017490813821283 34.0 28.5 36.5 2.0 39.0 74-75 28.53582014679578 34.0 27.5 36.0 2.0 38.5 76-77 27.21918121717097 31.5 26.0 34.5 2.0 36.5 78-79 27.93278024024123 33.5 27.0 35.0 2.0 37.0 80-81 27.70282277037208 34.0 26.0 35.0 2.0 37.0 82-83 27.281080591210927 33.0 26.0 35.0 2.0 36.0 84-85 26.83342121306991 33.0 25.0 35.0 2.0 36.0 86-87 26.545365167767912 33.0 25.0 35.0 2.0 35.5 88-89 26.055396273533006 32.5 24.0 35.0 2.0 35.0 90-91 25.43712479471843 32.0 21.0 34.0 2.0 35.0 92-93 24.937450682074914 32.0 19.0 34.0 2.0 35.0 94-95 24.21014333551202 31.0 11.0 34.0 2.0 35.0 96-97 23.402305195340176 31.0 2.0 34.0 2.0 35.0 98-99 22.46955882541409 31.0 2.0 34.0 2.0 35.0 100-101 19.009869992914695 24.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19424645364612303 1101 2 0.28201580118478375 1101 3 0.30866394147053455 1101 4 0.33155458884549915 1101 5 0.0662770758007909 1101 6 0.11926347084254019 1101 7 0.12275660212143436 1101 8 0.22886024415143424 1101 9 0.2026517760390334 1101 10-11 0.18689310856188257 1101 12-13 0.2781108280061986 1101 14-15 0.30498894635904605 1101 16-17 0.4715468893765902 1101 18-19 0.3405546104758912 1101 20-21 0.25213743230456487 1101 22-23 0.21755676134485924 1101 24-25 0.2408442460071214 1101 26-27 0.20755262035658006 1101 28-29 0.2734639803712611 1101 30-31 0.4047077119180855 1101 32-33 0.4751123120956464 1101 34-35 0.17142372013304907 1101 36-37 0.33549699088132456 1101 38-39 0.5002189797395786 1101 40-41 0.34510206765564533 1101 42-43 0.45973005607005035 1101 44-45 0.6178861005299083 1101 46-47 0.47876567092416167 1101 48-49 0.7989041683226858 1101 50-51 0.7981732117835918 1101 52-53 0.7844799649579457 1101 54-55 0.727346579337663 1101 56-57 0.6643779276340211 1101 58-59 0.6950125144556765 1101 60-61 0.5054536795102003 1101 62-63 0.39199483919563605 1101 64-65 0.7095355828875078 1101 66-67 0.8858438391152674 1101 68-69 0.6263825314854685 1101 70-71 0.5622405967361104 1101 72-73 0.6764816858338172 1101 74-75 0.5583971714710856 1101 76-77 0.6801246713954328 1101 78-79 0.34256929148063264 1101 80-81 0.4781437131771398 1101 82-83 0.39796878889497833 1101 84-85 0.3648915271170807 1101 86-87 0.6930339747110565 1101 88-89 0.2645785951430284 1101 90-91 0.5320027097910227 1101 92-93 0.7635742863376294 1101 94-95 0.6438447294660037 1101 96-97 0.39921580402209145 1101 98-99 0.0852928031359923 1101 100-101 0.980382468758421 1102 1 0.09642238552502391 1102 2 0.19579289317969284 1102 3 0.23785686076246293 1102 4 0.12890575253329928 1102 5 -0.025246362257298927 1102 6 0.029754519947452707 1102 7 0.14768766665645927 1102 8 0.0423415922862489 1102 9 0.061909130345895846 1102 10-11 0.21908918531241284 1102 12-13 0.2516939005991716 1102 14-15 0.3500434518095972 1102 16-17 0.3317757694074501 1102 18-19 0.11918247326217113 1102 20-21 0.07701992055339701 1102 22-23 0.08669367503622993 1102 24-25 0.11865345549949069 1102 26-27 0.10085623643246322 1102 28-29 0.34092072604582313 1102 30-31 0.5321133096207191 1102 32-33 0.6303135465048015 1102 34-35 0.6925544046300729 1102 36-37 0.547439613715035 1102 38-39 0.44532777633352794 1102 40-41 0.44297614077724035 1102 42-43 0.7174915465048102 1102 44-45 0.8684325837496587 1102 46-47 0.7739309017329532 1102 48-49 1.25369964787064 1102 50-51 1.6882550771129843 1102 52-53 1.747854873877408 1102 54-55 1.6596826700896017 1102 56-57 1.8082065962151717 1102 58-59 1.7192577961266977 1102 60-61 1.716067598044912 1102 62-63 1.5587329415773006 1102 64-65 1.5801797901653778 1102 66-67 1.6857309706855688 1102 68-69 1.6047803712694453 1102 70-71 1.506899348325554 1102 72-73 1.4191702404035595 1102 74-75 1.4176688129209474 1102 76-77 1.1722238813164232 1102 78-79 1.2029624736559938 1102 80-81 1.608192432334775 1102 82-83 1.455710277329537 1102 84-85 1.266517403990484 1102 86-87 1.510851470746374 1102 88-89 1.479435795148742 1102 90-91 1.2690978478762602 1102 92-93 1.4780528770538446 1102 94-95 1.5962756868474592 1102 96-97 1.807506633104996 1102 98-99 1.4436143495763503 1102 100-101 1.6762663428601172 1103 1 0.13824358257010516 1103 2 0.20420752995254077 1103 3 0.1151069738334769 1103 4 0.0590472528240511 1103 5 0.16214568185115752 1103 6 0.18279236104499574 1103 7 0.14773074132639863 1103 8 0.1506593110517329 1103 9 0.1384958934998224 1103 10-11 0.15875234139348038 1103 12-13 0.054725280496221274 1103 14-15 0.13523868775109804 1103 16-17 0.1921363043948574 1103 18-19 0.00934960298154408 1103 20-21 0.2109659910666295 1103 22-23 0.10572729481611276 1103 24-25 0.16444829084657187 1103 26-27 0.11109185487704565 1103 28-29 -0.031209962502771305 1103 30-31 0.09130759791948151 1103 32-33 0.01738468432220941 1103 34-35 -0.16697586609422643 1103 36-37 -0.08625985431279304 1103 38-39 -0.20174960731102232 1103 40-41 -0.19998438128775575 1103 42-43 0.014288944794749625 1103 44-45 0.2681805969305131 1103 46-47 0.43791617019583384 1103 48-49 0.28066439125329623 1103 50-51 0.05564539823464543 1103 52-53 0.1501938284551727 1103 54-55 0.2038955881831157 1103 56-57 0.027481434146721995 1103 58-59 0.10905017621758617 1103 60-61 -0.04498859286202972 1103 62-63 0.0928297734353265 1103 64-65 -0.11006056649289064 1103 66-67 -0.2613410286252389 1103 68-69 -0.006655953599715048 1103 70-71 -0.199113875598119 1103 72-73 0.06275857578294008 1103 74-75 -0.1832249864803117 1103 76-77 -0.12612097449111914 1103 78-79 -0.08186124325727562 1103 80-81 0.04926089952264334 1103 82-83 -0.08432870524675806 1103 84-85 0.09528482231014124 1103 86-87 0.021089242924446694 1103 88-89 0.12029858078764732 1103 90-91 -0.06145609237917071 1103 92-93 0.14816336358223836 1103 94-95 0.1428620192546859 1103 96-97 0.17304151281898328 1103 98-99 0.3781203907988093 1103 100-101 0.3466040482724466 1104 1 0.04405662263295085 1104 2 0.06353935380541031 1104 3 0.09225220741787155 1104 4 0.06612178727255014 1104 5 -0.008354757130405233 1104 6 0.009708862130899831 1104 7 -0.07661194154407269 1104 8 -0.010160429256018233 1104 9 0.05906333493044258 1104 10-11 0.035248344350826244 1104 12-13 0.06916315305294773 1104 14-15 0.19475996680475305 1104 16-17 -0.028181610673762236 1104 18-19 0.11382259406327933 1104 20-21 -0.0704789514036861 1104 22-23 0.10627024569638621 1104 24-25 -0.13541308339676306 1104 26-27 -0.19253320865976065 1104 28-29 0.020157811629658795 1104 30-31 0.2816777286897789 1104 32-33 0.393159833344896 1104 34-35 0.20196965398339728 1104 36-37 0.13303685101506346 1104 38-39 0.17288418964845675 1104 40-41 0.14695198948993493 1104 42-43 0.2923098310754142 1104 44-45 0.6917308025587161 1104 46-47 0.7221336277994581 1104 48-49 0.7399777316719494 1104 50-51 0.8287935891685265 1104 52-53 0.9652901733560526 1104 54-55 0.5438706355469094 1104 56-57 0.9816124816953575 1104 58-59 1.0297007931022009 1104 60-61 0.7213738672830665 1104 62-63 0.9620987536118442 1104 64-65 0.8064774862773163 1104 66-67 0.9761942054212511 1104 68-69 0.9318681441776526 1104 70-71 0.6592175323774825 1104 72-73 0.9109656826633987 1104 74-75 0.8443315035155265 1104 76-77 0.631143015158159 1104 78-79 0.5834915592934387 1104 80-81 0.6650134063384101 1104 82-83 0.6088717013887965 1104 84-85 0.16585688459931447 1104 86-87 0.28039565205526173 1104 88-89 0.5671171196721723 1104 90-91 0.49758461738470317 1104 92-93 0.5913776327934563 1104 94-95 0.7505026088762392 1104 96-97 0.7794876105039314 1104 98-99 0.4460100446965036 1104 100-101 0.8648471978496417 1105 1 0.023751229520534878 1105 2 0.14639013544360324 1105 3 0.17163912787806623 1105 4 0.132513256140804 1105 5 -0.03867429235189945 1105 6 0.07310468829761874 1105 7 0.1523373379727957 1105 8 0.04420546915012835 1105 9 0.10834571844915075 1105 10-11 0.1736546815445763 1105 12-13 0.038791062696333256 1105 14-15 0.19670450680398233 1105 16-17 0.2720515430165662 1105 18-19 0.09388986463623183 1105 20-21 0.0010318653986729487 1105 22-23 -0.18203528119272505 1105 24-25 -0.02971515120244561 1105 26-27 0.27517882742172617 1105 28-29 0.09077041339551783 1105 30-31 0.07983256567330699 1105 32-33 0.08795544164669877 1105 34-35 -0.16411747287513379 1105 36-37 -0.16800851839975905 1105 38-39 -0.4152722717665185 1105 40-41 -0.46196613916503537 1105 42-43 -0.5046004047538091 1105 44-45 -0.4981198453027673 1105 46-47 -0.27091237227698883 1105 48-49 -0.2607604290894372 1105 50-51 -0.019587234895993788 1105 52-53 -0.13439019586765966 1105 54-55 -0.25496980706287786 1105 56-57 0.08387999688856951 1105 58-59 -0.14556102702545815 1105 60-61 -0.41905345374280856 1105 62-63 -0.18462323927888136 1105 64-65 -0.3373085856563307 1105 66-67 -0.32320762158635574 1105 68-69 -0.1659727953170531 1105 70-71 -0.5540774834846118 1105 72-73 -0.028180248613598025 1105 74-75 0.0843054170575499 1105 76-77 0.011689970782516212 1105 78-79 0.23128137697489493 1105 80-81 0.03621271452172081 1105 82-83 -0.17198532953273826 1105 84-85 -0.03730454566479935 1105 86-87 0.34620901443725316 1105 88-89 0.27861809433104057 1105 90-91 0.2590870253654387 1105 92-93 0.5971706420049436 1105 94-95 0.7023262720647097 1105 96-97 0.7808612106262451 1105 98-99 0.6857880709167361 1105 100-101 0.4767012474616834 1106 1 0.1470764673266487 1106 2 0.2924385062187511 1106 3 0.3365652561921735 1106 4 0.09007245468360736 1106 5 0.13860763438358248 1106 6 0.1302844090019306 1106 7 0.1488562612676816 1106 8 0.11422671990908384 1106 9 0.2216375622054727 1106 10-11 0.25103274266580655 1106 12-13 0.10073702505213333 1106 14-15 0.3821679757374028 1106 16-17 0.32604870958203236 1106 18-19 0.18758547281271376 1106 20-21 0.08427159419851193 1106 22-23 0.204295927064706 1106 24-25 0.2926841606777444 1106 26-27 0.32813369485747046 1106 28-29 0.3986407837658845 1106 30-31 0.48056622282772565 1106 32-33 0.6208713493576852 1106 34-35 0.324166781324422 1106 36-37 0.45991093110438896 1106 38-39 0.5249447895570967 1106 40-41 0.5476422397056311 1106 42-43 0.8679835863411114 1106 44-45 0.902252042978958 1106 46-47 0.8301476358185411 1106 48-49 0.6557316777715201 1106 50-51 0.8960295430841683 1106 52-53 0.9868833317583281 1106 54-55 1.1072721480069418 1106 56-57 0.929402158394339 1106 58-59 1.113723530756367 1106 60-61 0.8032497237516232 1106 62-63 1.0028615816075792 1106 64-65 0.9829049338741278 1106 66-67 0.8552481565961969 1106 68-69 0.8092726003518429 1106 70-71 0.855730753714333 1106 72-73 1.0129298505566133 1106 74-75 0.7639122780168712 1106 76-77 0.7127828669573795 1106 78-79 0.7018187175942039 1106 80-81 0.5237531921578267 1106 82-83 0.551357617239173 1106 84-85 0.7161436429609864 1106 86-87 0.7440889982461982 1106 88-89 0.5770394665229084 1106 90-91 0.9973156228153499 1106 92-93 0.8896240927234196 1106 94-95 0.8471071868592865 1106 96-97 1.142566837017661 1106 98-99 0.5988446384131976 1106 100-101 0.19572320657927378 1107 1 0.14109609631715614 1107 2 0.14567944668466737 1107 3 0.13269338388845853 1107 4 -0.015147562572650486 1107 5 0.15495793636804933 1107 6 0.0022591717284186075 1107 7 0.1440730429760464 1107 8 -0.04199092461205822 1107 9 0.03642401604498957 1107 10-11 0.1642634372300833 1107 12-13 0.0945598248795747 1107 14-15 0.28285013708338624 1107 16-17 0.34275492183397915 1107 18-19 0.19755904917164457 1107 20-21 0.20428885045390643 1107 22-23 0.22406242835892698 1107 24-25 0.08686826335246423 1107 26-27 0.054225692269028514 1107 28-29 0.19469341534482965 1107 30-31 0.14700280608916216 1107 32-33 0.19690402839133725 1107 34-35 0.28875370971096004 1107 36-37 0.3237779500862672 1107 38-39 0.3733485859332859 1107 40-41 0.5556690947530925 1107 42-43 0.30309736977510937 1107 44-45 0.12091036912217845 1107 46-47 0.16437433987376693 1107 48-49 -0.12583109185747077 1107 50-51 0.06278159226449986 1107 52-53 -0.1148665603900838 1107 54-55 0.2919356510267903 1107 56-57 0.12944529903281676 1107 58-59 0.3526013348978623 1107 60-61 0.037066591741268695 1107 62-63 -0.19178089035100143 1107 64-65 0.22883623240734607 1107 66-67 0.3729627812726406 1107 68-69 0.5389695373665084 1107 70-71 0.37235607726912434 1107 72-73 0.3612365804962181 1107 74-75 0.46594528060531104 1107 76-77 0.23181058481760175 1107 78-79 0.7247938038754818 1107 80-81 0.4335892577315974 1107 82-83 0.37435157754978476 1107 84-85 0.2979679527107706 1107 86-87 0.3144513796094408 1107 88-89 0.4182684354616448 1107 90-91 0.5977200663011111 1107 92-93 0.85153630152411 1107 94-95 0.7803497450991479 1107 96-97 1.0781524711850459 1107 98-99 0.9243865926841188 1107 100-101 0.4279276932056746 1108 1 0.13734124015213922 1108 2 0.12511879087114863 1108 3 -0.03875814695975066 1108 4 -0.07418465442354005 1108 5 -0.07792986309546279 1108 6 0.09389327125668245 1108 7 0.15392123000777502 1108 8 -0.018632192764862054 1108 9 -0.06395407108848161 1108 10-11 0.023453440467527287 1108 12-13 0.11173417276598485 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-1.8012179894253784 2316 56-57 -1.6585393855423618 2316 58-59 -1.8838818150960535 2316 60-61 -1.551327567733626 2316 62-63 -1.5001918390564875 2316 64-65 -1.2519188271526396 2316 66-67 -1.4341547889230526 2316 68-69 -1.9377648433311947 2316 70-71 -1.538436891006068 2316 72-73 -1.3723160510827306 2316 74-75 -1.523058153210176 2316 76-77 -1.460788859086616 2316 78-79 -1.5135543941310807 2316 80-81 -1.5225308346471564 2316 82-83 -1.6341873795427162 2316 84-85 -1.8478229643914545 2316 86-87 -2.0577377537319954 2316 88-89 -2.2760821024014852 2316 90-91 -2.556235658750545 2316 92-93 -2.5103247960813384 2316 94-95 -2.363826822908628 2316 96-97 -2.6946345410916024 2316 98-99 -1.9790734322338324 2316 100-101 -2.633470750941644 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 7.0 5 10.0 6 29.0 7 84.0 8 222.0 9 403.0 10 899.0 11 1490.0 12 2387.0 13 3561.0 14 4560.0 15 6827.0 16 9788.0 17 14000.0 18 11446.0 19 5605.0 20 4133.0 21 4117.0 22 4287.0 23 4596.0 24 5030.0 25 5763.0 26 6850.0 27 8235.0 28 10305.0 29 12427.0 30 15265.0 31 19025.0 32 23712.0 33 31373.0 34 42316.0 35 58899.0 36 80663.0 37 95828.0 38 49897.0 39 2160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.62078272110388 19.646028717595662 15.735352817744705 16.997835743555754 2 15.763406090989488 24.31122528385007 37.978324675776484 21.947043949383964 3 22.64789351300877 28.465679184136803 30.654375011443324 18.2320522914111 4 11.876235811058221 18.02068839253021 39.676858293665326 30.426217502746244 5 10.77415740211803 41.41271550145743 32.96311757554452 14.850009520880022 6 26.79141927605405 39.83240601902963 16.482760009593537 16.893414695322782 7 25.359804759803662 37.87062956907647 20.043610478964627 16.72595519215524 8 26.95457466499937 30.27299873854114 19.170879158099794 23.601547438359702 9 27.297643175314583 18.472869877572542 21.390294049260252 32.83919289785262 10-11 25.108247696360863 27.601285973478628 24.92662957409452 22.363836756065993 12-13 30.0090076729995 26.20399816184884 25.49235537833142 18.294638786820236 14-15 24.344057751845025 26.630037770116754 29.90549232056448 19.12041215747374 16-17 19.153571869950703 29.95409001652393 29.499567448311222 21.392770665214147 18-19 18.6456519629652 28.4247760390634 34.34873276466571 18.58083923330569 20-21 21.924120853720495 24.20800168437241 33.90467733741001 19.963200124497092 22-23 20.290261040867964 25.205969263893312 34.43558929179528 20.06818040344344 24-25 21.693526608964707 25.184479972903574 34.65761011355782 18.464383304573893 26-27 20.790003661662396 23.930977663859395 36.94049798608569 18.338520688392528 28-29 18.745863469550102 27.656053042792895 35.34379774460111 18.254285743055892 30-31 19.726598973990928 25.28616205963449 34.65947996499412 20.327759001380464 32-33 19.579699385093388 26.070410789107264 34.8679603171479 19.481929508651444 34-35 21.407268126834285 24.8201643008973 34.80656105076411 18.9660065215043 36-37 20.90178828892744 24.225524883626193 35.90638831547486 18.96629851197151 38-39 19.872310702080583 25.967030381593993 35.58931796813993 18.5713409481855 40-41 19.74412209491796 26.62953727402883 34.46634736853669 19.159993262516522 42-43 19.157457450859773 27.324548876585524 32.47447783929461 21.0435158332601 44-45 20.786483938004082 24.941546968406982 32.69176897091538 21.580200122673553 46-47 22.291061349210274 27.27430012028539 31.591758345371023 18.84288018513331 48-49 21.91444115598089 27.143433869994716 30.832748214663336 20.10937675936106 50-51 19.58518553779229 28.03901896022673 32.478725585687194 19.897069916293788 52-53 21.11562209640286 29.73558099405435 28.800388137990378 20.348408771552414 54-55 19.837330648114243 27.929146832662028 29.781948004393993 22.451574514829733 56-57 22.06309155196502 27.318462555412935 28.989593995535838 21.62885189708621 58-59 23.83591012992767 26.71303039152234 28.489889479547813 20.961169999002177 60-61 21.259768995357753 28.017529035784023 31.56022882587135 19.162473142986876 62-63 20.88072134749176 27.91083852068839 30.883559135847676 20.324880995972173 64-65 20.286425458250523 30.219313944749032 29.70931770724147 19.784942889758973 66-67 21.624216969931094 27.79069032974457 28.51460694934323 22.070485750981106 68-69 20.993224891315393 28.298002253792404 29.089575889730778 21.61919696516142 70-71 23.029812009941914 28.353954107905473 28.421332088305434 20.19490179384718 72-73 22.255635931546752 26.929069560934703 30.489506264383913 20.325788243134628 74-75 20.263279077836604 30.99905612097764 28.623886639305468 20.113778161880287 76-77 21.011548126347396 28.293596122246278 28.681279951665395 22.013575799740934 78-79 20.914239259995714 28.655116916502337 28.97178294232998 21.45886088117197 80-81 22.15921938322926 28.449138430582394 28.369220234591484 21.022421951596854 82-83 21.61776687386142 27.804590019865067 29.568369691588014 21.0092734146855 84-85 20.473698761056113 28.8865982778918 29.271903052228982 21.367799908823105 86-87 20.42728055825858 29.699092912192178 27.80966992769112 22.063956601858116 88-89 19.83036960750658 29.650074379219593 26.87181599725369 23.647740016020137 90-91 20.894339087168078 27.363718341320958 28.013073151975394 23.728869419535574 92-93 21.922579771067838 28.74065340139293 28.335664642943453 21.00110218459578 94-95 21.07537728922975 28.003896015920937 28.589579988246044 22.33114670660327 96-97 18.870010984987186 28.437843280849506 30.579183449285974 22.112962284877334 98-99 20.197089880491948 29.769176900297055 27.337547315760325 22.696185903450676 100-101 19.109886055821388 28.957035895116796 27.970471732744283 23.962606316317537 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 692.0 1 497.5 2 373.5 3 452.0 4 399.0 5 381.0 6 572.5 7 1072.5 8 1706.0 9 2054.5 10 1905.5 11 1634.5 12 1632.5 13 1706.5 14 1814.0 15 1962.0 16 2054.0 17 2115.5 18 2220.0 19 2318.0 20 2436.5 21 2623.5 22 2886.0 23 3205.0 24 3516.5 25 3908.0 26 4492.0 27 5238.5 28 5906.0 29 6800.5 30 7908.0 31 8888.5 32 10451.5 33 11822.0 34 12590.0 35 13660.0 36 15611.0 37 17088.5 38 16844.5 39 16086.0 40 15097.0 41 14266.0 42 13572.0 43 13726.5 44 14408.0 45 14553.5 46 15071.5 47 23769.0 48 42358.0 49 39804.5 50 21221.0 51 13616.0 52 11079.0 53 10299.5 54 9839.5 55 9340.0 56 8881.0 57 8398.0 58 8264.0 59 7894.5 60 6894.0 61 5910.0 62 5011.0 63 3609.0 64 2355.5 65 1771.5 66 1299.5 67 991.0 68 746.5 69 517.0 70 405.0 71 332.5 72 330.0 73 333.0 74 262.5 75 240.5 76 196.0 77 127.0 78 87.0 79 47.0 80 29.5 81 27.5 82 22.5 83 9.5 84 5.0 85 2.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010069553149847768 2 0.009154139227134334 3 0.005675566320823286 4 1.8308278454268667E-4 5 0.0060417318899086605 6 0.0 7 3.6616556908537334E-4 8 0.0014646622763414933 9 0.0 10-11 0.0 12-13 0.0 14-15 3.6616556908537334E-4 16-17 0.0043024454367531365 18-19 0.00219699341451224 20-21 6.407897458994033E-4 22-23 5.4924835362806E-4 24-25 6.407897458994033E-4 26-27 1.8308278454268667E-4 28-29 9.154139227134333E-5 30-31 0.0012815794917988067 32-33 0.003753197083125077 34-35 0.0014646622763414933 36-37 0.0015562036686128367 38-39 0.00576710771309463 40-41 5.4924835362806E-4 42-43 0.00164774506088418 44-45 0.00659098024353672 46-47 0.0 48-49 0.002837783160411643 50-51 9.154139227134333E-4 52-53 6.407897458994033E-4 54-55 1.8308278454268667E-4 56-57 0.013548126056158813 58-59 0.0022885348067835836 60-61 0.003936279867667764 62-63 1.8308278454268667E-4 64-65 0.003753197083125077 66-67 0.0010069553149847767 68-69 0.0019223692376982102 70-71 0.005217859359466569 72-73 0.00329549012176836 74-75 0.009245680619405677 76-77 0.0015562036686128367 78-79 0.007689476950792841 80-81 0.0032039487294970165 82-83 0.0029293245526829867 84-85 0.0018308278454268667 86-87 4.5770696135671667E-4 88-89 0.0024716175913262702 90-91 0.007964101127606869 92-93 0.0023800761990549267 94-95 3.6616556908537334E-4 96-97 1.8308278454268667E-4 98-99 6.407897458994033E-4 100-101 0.0022885348067835836 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 546201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.091368188384244 #Duplication Level Percentage of deduplicated Percentage of total 1 78.53685554598682 35.4133426978204 2 11.749493415734346 10.596014672717477 3 3.843801012290476 5.199667400642219 4 1.7511987472075625 3.1585578992549372 5 0.9706653251078387 2.188431378106763 6 0.5925760785415407 1.6032039682287311 7 0.4049273053450989 1.2781108350392303 8 0.2915247421860135 1.0516199588746662 9 0.20266189144236185 0.8224471768303707 >10 1.4237615446223664 12.178837882298224 >50 0.14523200594741803 4.505733121719528 >100 0.07180009282021373 6.107039842232038 >500 0.006119326092631851 1.9647969081864032 >1k 0.007751146384000344 8.06613422217635 >5k 0.0012238652185263702 3.438256624933252 >10k+ 4.079550728421234E-4 2.427805410939439 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13198 2.416326590394379 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7185 1.3154498069392038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6307 1.154703122110725 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5199 0.9518473968374279 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4394 0.8044657552805652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3853 0.7054179688429718 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3479 0.636945007424007 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3304 0.6049055201290368 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3283 0.6010607816536403 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3201 0.5860479933211401 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3192 0.5844002482602558 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2749 0.5032945747078457 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2683 0.49121111092802827 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1909 0.3495050356919889 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1872 0.34273097266390945 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1696 0.3105084025843966 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1390 0.2544850705143345 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1316 0.24093694445817565 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1211 0.22171325208119352 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1178 0.21567152019128488 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1079 0.1975463245215589 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1036 0.1896737647862234 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1024 0.18747677137171115 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 999 0.18289970175814396 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 976 0.1786887977136622 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 925 0.16935157570198517 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 912 0.16697149950293022 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 809 0.1481139726950335 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 789 0.14445231700417976 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 753 0.13786133676064308 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 661 0.1210177205827159 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.11424365755463649 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 595 0.10893425680289857 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 555 0.1016109454211911 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.8308278454268667E-4 0.0 18-19 0.0 0.0 0.0 1.8308278454268667E-4 0.0 20-21 0.0 0.0 0.0 2.7462417681403E-4 0.0 22-23 0.0 0.0 0.0 5.4924835362806E-4 0.0 24-25 0.0 0.0 0.0 0.0014646622763414933 0.0 26-27 0.0 0.0 0.0 0.0023800761990549267 0.0 28-29 0.0 0.0 0.0 0.005584024928551944 0.0 30-31 0.0 0.0 0.0 0.01318196048707344 0.0 32-33 0.0 0.0 0.0 0.022336099714207774 0.0 34-35 0.0 0.0 0.0 0.03130715615679942 0.0 36-37 0.0 0.0 0.0 0.03744042943897942 0.0 38-39 0.0 0.0 0.0 0.04329907854434539 0.0 40-41 0.0 0.0 0.0 0.0515378038487663 0.0 42-43 0.0 0.0 0.0 0.060966567252714654 0.0 44-45 0.0 0.0 0.0 0.07195153432527586 0.0 46-47 0.0 0.0 0.0 0.08604890873506274 0.0 48-49 0.0 0.0 0.0 0.10014628314484961 0.0 50-51 0.0 0.0 0.0 0.12312317260495678 0.0 52-53 0.0 0.0 0.0 0.16935157570198517 0.0 54-55 0.0 0.0 0.0 0.2188754689207819 0.0 56-57 0.0 0.0 0.0 0.2912847102074145 0.0 58-59 0.0 0.0 0.0 0.38456538893191333 0.0 60-61 0.0 0.0 0.0 0.5224267256925564 0.0 62-63 0.0 0.0 0.0 0.615890487201598 0.0 64-65 0.0 0.0 0.0 0.6984608230303497 0.0 66-67 0.0 0.0 0.0 0.8890500017392865 0.0 68-69 0.0 0.0 0.0 1.1269660802525077 0.0 70-71 0.0 0.0 0.0 1.3046479226511851 0.0 72-73 0.0 0.0 0.0 1.4487340740862797 0.0 74-75 0.0 0.0 0.0 1.7840501939762103 0.0 76-77 0.0 0.0 0.0 1.9999963383443091 0.0 78-79 0.0 0.0 0.0 2.151131176984297 0.0 80-81 0.0 0.0 0.0 2.3178280523104133 0.0 82-83 0.0 0.0 0.0 2.6041695273351753 0.0 84-85 0.0 0.0 0.0 2.956603887579847 0.0 86-87 0.0 0.0 0.0 3.3790674129120966 0.0 88-89 0.0 0.0 0.0 4.05189664610647 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 75 0.0 41.162663 94-95 GGTAAGG 195 1.6370905E-11 29.238632 1 GTATAGG 225 3.6379788E-12 27.451824 1 AGGTAAG 160 6.21294E-8 26.725937 2 TAAGGTG 270 0.0 26.393568 5 GTAAGGT 275 0.0 25.913685 4 GTATATG 225 1.0186341E-10 25.340145 1 CACGCAT 95 0.0012264265 24.997566 9 CTCCATA 115 1.2782132E-4 24.789274 2 AGTACTT 6400 0.0 24.712439 12-13 GAGTACT 6360 0.0 23.897045 12-13 ATGCACG 100 0.0016534746 23.747688 6 GTACATG 6075 0.0 22.680994 1 GTACTTT 6670 0.0 22.679577 14-15 AGTATGG 105 0.0021913284 22.625132 2 TACTTTT 7055 0.0 22.350765 14-15 GACAGGT 130 2.9407087E-4 21.920944 7 TACATGG 6240 0.0 21.85283 2 ACCCTTG 305 9.094947E-12 21.801157 7 ATGGGAT 1525 0.0 21.495615 5 >>END_MODULE