##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936562_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609635 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.565256259893214 31.0 31.0 34.0 30.0 34.0 2 31.561972327704282 31.0 31.0 34.0 30.0 34.0 3 31.573843365292348 31.0 31.0 34.0 28.0 34.0 4 35.24386723203228 37.0 35.0 37.0 33.0 37.0 5 35.216014500479794 37.0 35.0 37.0 33.0 37.0 6 35.38296029591477 37.0 35.0 37.0 33.0 37.0 7 35.24654588401256 37.0 35.0 37.0 32.0 37.0 8 35.24387215300959 37.0 35.0 37.0 32.0 37.0 9 36.72630836484126 39.0 37.0 39.0 32.0 39.0 10-11 36.60563779966701 39.0 37.0 39.0 32.0 39.0 12-13 36.54143544908019 39.0 37.0 39.0 32.0 39.0 14-15 37.468753434432074 39.5 37.0 41.0 32.0 41.0 16-17 37.49308930753648 39.0 37.0 41.0 32.0 41.0 18-19 37.60375306535878 40.0 37.0 41.0 32.0 41.0 20-21 37.558970531547565 40.0 37.0 41.0 32.0 41.0 22-23 37.44384918844882 39.5 37.0 41.0 32.0 41.0 24-25 37.26749940538191 39.0 36.5 41.0 31.5 41.0 26-27 37.04861023399247 39.0 36.0 41.0 31.0 41.0 28-29 37.15601384434949 39.0 36.0 41.0 31.0 41.0 30-31 37.037737334634656 39.0 36.0 41.0 31.0 41.0 32-33 36.75101495156939 39.0 35.0 41.0 31.0 41.0 34-35 36.481502866469285 39.0 35.0 40.0 30.0 41.0 36-37 36.17491367785642 38.0 35.0 40.0 30.0 41.0 38-39 35.930001558309485 38.0 35.0 40.0 30.0 41.0 40-41 35.7766491425197 38.0 35.0 40.0 29.5 41.0 42-43 35.38477367605206 38.0 35.0 40.0 27.5 41.0 44-45 34.887082434571504 38.0 34.5 40.0 25.0 41.0 46-47 34.224474480631855 38.0 33.5 40.0 23.0 41.0 48-49 33.67042164573884 38.0 33.0 40.0 18.5 41.0 50-51 32.87191352202548 37.5 32.0 39.5 15.0 40.5 52-53 33.04782205746061 37.5 33.0 39.5 14.5 40.5 54-55 33.38599653891263 38.0 33.0 40.0 12.5 41.0 56-57 33.20819670786618 38.0 33.0 40.0 9.0 41.0 58-59 32.96861728739327 37.5 33.0 40.0 8.0 41.0 60-61 32.5314680095467 37.0 32.0 40.0 7.0 41.0 62-63 32.26505449982366 36.0 32.0 40.0 3.5 41.0 64-65 31.95936338956917 36.0 31.0 39.0 2.0 41.0 66-67 31.497314786716643 35.5 31.0 39.0 2.0 41.0 68-69 31.080131554126652 35.0 30.5 39.0 2.0 40.0 70-71 30.580884463654485 35.0 30.0 37.5 2.0 40.0 72-73 30.171819203293772 35.0 29.5 37.0 2.0 39.5 74-75 29.771555931007896 35.0 29.0 37.0 2.0 39.0 76-77 29.36485520024277 34.0 29.0 36.0 2.0 39.0 78-79 28.867206607232198 34.0 28.5 36.0 2.0 38.5 80-81 28.362308594486866 34.0 27.5 35.0 2.0 37.0 82-83 27.912743690897013 34.0 26.5 35.0 2.0 37.0 84-85 27.486695317690092 34.0 26.0 35.0 2.0 36.0 86-87 27.242628786076914 34.0 26.0 35.0 2.0 36.0 88-89 26.79804391152083 33.5 25.0 35.0 2.0 36.0 90-91 26.34150926373978 33.0 24.5 35.0 2.0 35.0 92-93 25.907173964749397 33.0 22.5 35.0 2.0 35.0 94-95 25.475721538297506 33.0 20.0 35.0 2.0 35.0 96-97 24.902594175203195 32.0 14.0 35.0 2.0 35.0 98-99 24.293907009932173 32.0 2.0 35.0 2.0 35.0 100-101 22.359404397713384 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07300321009575939 1101 2 0.24221033144452164 1101 3 0.23154977211725125 1101 4 0.11378754003156644 1101 5 0.23487624399508888 1101 6 0.3303635472977149 1101 7 0.35389675214072724 1101 8 0.1979529841616312 1101 9 0.5014756500425293 1101 10-11 0.32490881433412966 1101 12-13 0.6285722414349877 1101 14-15 0.716671306926493 1101 16-17 0.5822568892500826 1101 18-19 0.5479487406839638 1101 20-21 0.3417433715834832 1101 22-23 0.4626416972887313 1101 24-25 0.3704295400262936 1101 26-27 0.48305641007624445 1101 28-29 0.42638127759172306 1101 30-31 0.23735066325033216 1101 32-33 0.17757318039868153 1101 34-35 0.3076492313153594 1101 36-37 0.21971078963311896 1101 38-39 0.2163431253771435 1101 40-41 -0.10319890092916495 1101 42-43 -0.07084968302316241 1101 44-45 0.0866947258442039 1101 46-47 0.5149568067512007 1101 48-49 0.4204523174259336 1101 50-51 0.3625254263064974 1101 52-53 0.38245378173714784 1101 54-55 0.8272637044092548 1101 56-57 0.5445137785974552 1101 58-59 0.6776210893525771 1101 60-61 0.7515300743061673 1101 62-63 0.6341670067475036 1101 64-65 0.7000585363947387 1101 66-67 0.8794387240816093 1101 68-69 0.8078885930600919 1101 70-71 0.7928405404604391 1101 72-73 0.8954778179439415 1101 74-75 0.9267045919197088 1101 76-77 0.9059370860457534 1101 78-79 0.9981004161336209 1101 80-81 0.8280496643415063 1101 82-83 0.9831261922603503 1101 84-85 1.0777263341684389 1101 86-87 1.1975240054911112 1101 88-89 1.0390734549443827 1101 90-91 1.0233048780312899 1101 92-93 0.7749896881542071 1101 94-95 0.7308486336332081 1101 96-97 0.6560446286080186 1101 98-99 0.6357162493587118 1101 100-101 0.9851058532699781 1102 1 0.06826048470685464 1102 2 0.19939811418391784 1102 3 0.2787854928655804 1102 4 0.41663791799029326 1102 5 0.39562362455136935 1102 6 0.38136243822962257 1102 7 0.21884764669169954 1102 8 0.23208966221083926 1102 9 0.49495622675793527 1102 10-11 0.5528861514032783 1102 12-13 0.6612104006738164 1102 14-15 0.6614595989616348 1102 16-17 0.48456586686442193 1102 18-19 0.3226355383957866 1102 20-21 0.18637077578043204 1102 22-23 -0.11710084568765211 1102 24-25 0.3196477199871879 1102 26-27 0.29257396545668257 1102 28-29 0.2462580396966132 1102 30-31 0.49506853675818974 1102 32-33 0.4694222595803126 1102 34-35 0.34093495498228776 1102 36-37 0.041612330654047014 1102 38-39 0.07967054854498912 1102 40-41 -0.06927290933473529 1102 42-43 0.5347142635080857 1102 44-45 0.6622791755421318 1102 46-47 0.6183482202293007 1102 48-49 1.0162663150326807 1102 50-51 1.2657966299372347 1102 52-53 1.3349891332825408 1102 54-55 1.259278560084475 1102 56-57 1.2798961517113057 1102 58-59 1.2309384433496007 1102 60-61 0.9587835255509418 1102 62-63 0.9528599116303198 1102 64-65 0.857057339768641 1102 66-67 0.9023251102707945 1102 68-69 1.2584839875089173 1102 70-71 1.3600294541574272 1102 72-73 1.0931655569043386 1102 74-75 1.2752575134385467 1102 76-77 1.3627582196300843 1102 78-79 1.155874472696091 1102 80-81 1.2755376615980225 1102 82-83 1.2003858820131477 1102 84-85 1.1439958887900445 1102 86-87 1.1413236216951788 1102 88-89 0.9887905331336846 1102 90-91 1.0965389424739804 1102 92-93 1.4859853321433505 1102 94-95 0.9466389905780197 1102 96-97 0.5497501199543677 1102 98-99 0.6437943875836218 1102 100-101 0.7044423095055663 1103 1 0.10594921706056226 1103 2 0.28294195205452866 1103 3 0.3942640719238675 1103 4 0.19720137247801262 1103 5 0.3472623121578309 1103 6 0.03803916099875693 1103 7 0.09152548092479407 1103 8 0.023058656696825608 1103 9 0.35220888994105337 1103 10-11 0.0972238199271942 1103 12-13 0.3352474326320163 1103 14-15 0.5433981292309866 1103 16-17 0.45835805197369694 1103 18-19 0.32135537008838355 1103 20-21 0.12908798037640423 1103 22-23 -0.01872997037369828 1103 24-25 0.17424622167603587 1103 26-27 0.12588324112861926 1103 28-29 0.2954673846546143 1103 30-31 0.15735129607572418 1103 32-33 0.3176099959468317 1103 34-35 0.1675835663740699 1103 36-37 0.09205222499279841 1103 38-39 -0.012889499377870095 1103 40-41 -0.2520338507591404 1103 42-43 0.13613354524254362 1103 44-45 0.42589100036386185 1103 46-47 0.47203914664172686 1103 48-49 0.3243763932681958 1103 50-51 0.062282346903657526 1103 52-53 0.44825831686174666 1103 54-55 0.6270396097683602 1103 56-57 0.6600267487262315 1103 58-59 0.755006329258542 1103 60-61 0.9445474044158146 1103 62-63 0.6373087987228985 1103 64-65 0.7808210165491438 1103 66-67 0.8407498266309616 1103 68-69 0.7025427074488704 1103 70-71 0.7549789691549762 1103 72-73 0.5464299409800901 1103 74-75 0.6302556730274489 1103 76-77 0.5935800534012685 1103 78-79 0.7640238852080046 1103 80-81 0.9282259248273306 1103 82-83 0.6627341383138479 1103 84-85 0.38819047437276666 1103 86-87 0.5507420157019354 1103 88-89 0.6514855806235076 1103 90-91 0.9190087380800911 1103 92-93 1.0231372779270025 1103 94-95 0.6925143045305013 1103 96-97 0.5966400056323629 1103 98-99 0.7220001311097057 1103 100-101 1.1634155766322145 1104 1 0.08585680443083632 1104 2 0.10542169095217702 1104 3 -0.013530493333384186 1104 4 0.08071928659293803 1104 5 0.1397941248388932 1104 6 -0.15812864860188824 1104 7 0.1714569048860639 1104 8 0.26824549889859384 1104 9 0.2604105108292458 1104 10-11 0.1926719478380079 1104 12-13 0.028659365596190867 1104 14-15 0.2923933252411075 1104 16-17 -0.11199106240527357 1104 18-19 0.06256150159302365 1104 20-21 -0.01670211093607321 1104 22-23 0.1574930301030264 1104 24-25 0.14096025592738215 1104 26-27 0.030167409965024206 1104 28-29 0.014925377246932214 1104 30-31 0.09544950283064679 1104 32-33 0.2710323803393635 1104 34-35 0.23889714589660827 1104 36-37 0.11423308971097867 1104 38-39 0.17227405630807624 1104 40-41 -0.038942978339910894 1104 42-43 0.07889913987380481 1104 44-45 -0.32565929484315603 1104 46-47 0.05339709889519639 1104 48-49 0.04040375034032451 1104 50-51 -0.2936520474635671 1104 52-53 -0.08030341990895096 1104 54-55 0.08161898561639447 1104 56-57 0.23006507063022497 1104 58-59 0.2344390183927274 1104 60-61 0.22311647552218972 1104 62-63 0.17630729518408828 1104 64-65 0.09131553413321924 1104 66-67 -0.15876244004548568 1104 68-69 0.16675023247729115 1104 70-71 0.08051895962033839 1104 72-73 -0.017314604107738774 1104 74-75 -0.2687871961646735 1104 76-77 0.007748156375342319 1104 78-79 0.2006918965028781 1104 80-81 0.2959768507872127 1104 82-83 0.19335957379003332 1104 84-85 0.20626227751919757 1104 86-87 0.18671968264974836 1104 88-89 0.2882837763769288 1104 90-91 0.22414460664879599 1104 92-93 0.060586395973135865 1104 94-95 0.09727153801907562 1104 96-97 0.14521806129997117 1104 98-99 0.24137561242548244 1104 100-101 0.6166188477459329 1105 1 0.1390074162556374 1105 2 0.24073217774362377 1105 3 0.26427036817692695 1105 4 0.12832916727757748 1105 5 0.3137601218655419 1105 6 0.24236309843428927 1105 7 0.3707078483542148 1105 8 -0.10353813240548959 1105 9 0.35129794268987524 1105 10-11 0.36794069331126167 1105 12-13 0.217008010432842 1105 14-15 0.32845668055693267 1105 16-17 0.5773677604514376 1105 18-19 0.21614096257728477 1105 20-21 -0.16204004407372707 1105 22-23 -0.4246258659439377 1105 24-25 -0.1380785501565498 1105 26-27 -0.14833771716492805 1105 28-29 -0.1590082178432226 1105 30-31 -0.16794012092569233 1105 32-33 -0.29212757086712315 1105 34-35 -0.040276032923962646 1105 36-37 -0.022939555430639302 1105 38-39 -0.13893034273130667 1105 40-41 -0.0810149868787704 1105 42-43 -0.09640200753593575 1105 44-45 0.16572274304805745 1105 46-47 0.6381243508297842 1105 48-49 0.5953745885974868 1105 50-51 0.626203668619226 1105 52-53 0.6938150568188419 1105 54-55 0.9301027655881811 1105 56-57 1.039108069905943 1105 58-59 0.7339224934051103 1105 60-61 1.1145280563554962 1105 62-63 0.9652124909299005 1105 64-65 1.0772731066563743 1105 66-67 0.6728580379783793 1105 68-69 0.8838403479806658 1105 70-71 0.9152099976259045 1105 72-73 0.9146889791652377 1105 74-75 0.864232283117147 1105 76-77 1.2014488387220048 1105 78-79 0.933623699956545 1105 80-81 1.0536141778180514 1105 82-83 0.9017755002948142 1105 84-85 0.44016613906762814 1105 86-87 0.7397606996893558 1105 88-89 0.5558303779855898 1105 90-91 0.7164157917972709 1105 92-93 0.7789220800351941 1105 94-95 1.1957724719572447 1105 96-97 1.4701532574269862 1105 98-99 1.051120971324579 1105 100-101 0.7015576652658524 1106 1 -0.09488058035279323 1106 2 -0.15712322390487543 1106 3 -0.025505184787846247 1106 4 0.19279552468074002 1106 5 0.18381849603089506 1106 6 0.21211818782992253 1106 7 0.20534223530319196 1106 8 0.03957029189968608 1106 9 0.19608462424902484 1106 10-11 0.20708766413233803 1106 12-13 0.23210804548995156 1106 14-15 -0.09872134434176871 1106 16-17 0.07306205429524226 1106 18-19 0.06429169999951512 1106 20-21 0.28265736414594755 1106 22-23 0.28751958845329284 1106 24-25 0.07160781099690894 1106 26-27 0.03476434794346517 1106 28-29 -0.03976920172510745 1106 30-31 -0.0737037544728949 1106 32-33 -0.10578374214149733 1106 34-35 0.020068056691791014 1106 36-37 0.31863084006248243 1106 38-39 0.24460259872331847 1106 40-41 0.24535244425031522 1106 42-43 0.36850623251558545 1106 44-45 0.3387642327306608 1106 46-47 0.1923089876625781 1106 48-49 0.2291398853192348 1106 50-51 0.646634626493622 1106 52-53 0.3211416667962581 1106 54-55 0.2248270781019457 1106 56-57 0.38195540026621444 1106 58-59 0.434626489100431 1106 60-61 0.04264467424114571 1106 62-63 0.38169707803051267 1106 64-65 0.3632066448971649 1106 66-67 0.3213656649381136 1106 68-69 0.3684335632068638 1106 70-71 0.3409704515747194 1106 72-73 0.4497022682559546 1106 74-75 0.5686600776987873 1106 76-77 0.2210859161003853 1106 78-79 0.2957942242641636 1106 80-81 0.42962003154021744 1106 82-83 0.731459089490702 1106 84-85 1.018830626214715 1106 86-87 0.8623719244869612 1106 88-89 0.7685954464284812 1106 90-91 0.6610489965878692 1106 92-93 0.6139100953950987 1106 94-95 1.0309459638900336 1106 96-97 0.5573099219467466 1106 98-99 0.328838508847916 1106 100-101 0.45813189258267784 1107 1 0.27302276964558203 1107 2 0.37641200584493006 1107 3 0.4378335348599123 1107 4 0.4586996121342395 1107 5 0.44069418061700105 1107 6 0.2165835870859567 1107 7 0.34077641201045594 1107 8 0.2954753553967393 1107 9 0.22330539445583497 1107 10-11 0.2586688546885725 1107 12-13 0.517257813950728 1107 14-15 0.6467034515024537 1107 16-17 0.5999479873386591 1107 18-19 0.49397725130145886 1107 20-21 0.5355746498772689 1107 22-23 0.6153387275828663 1107 24-25 0.5925385227495568 1107 26-27 0.6069725922580176 1107 28-29 0.33744451927827157 1107 30-31 0.35228232063907683 1107 32-33 0.38287381840031287 1107 34-35 0.6722752707381474 1107 36-37 0.7991045465336555 1107 38-39 0.8962472321105182 1107 40-41 0.6856158675604078 1107 42-43 0.5761622096978556 1107 44-45 0.6679376322903607 1107 46-47 0.5031232791091824 1107 48-49 0.464157616777122 1107 50-51 0.3017171021738676 1107 52-53 0.5199780059822317 1107 54-55 0.556806602877181 1107 56-57 0.4671029997413214 1107 58-59 0.5034999179414328 1107 60-61 0.49314842116389457 1107 62-63 0.24818245364460978 1107 64-65 0.09210783675478851 1107 66-67 0.09676719841098347 1107 68-69 0.19236886236421213 1107 70-71 0.34002152034241107 1107 72-73 -0.017124451478558456 1107 74-75 0.10743611908303308 1107 76-77 0.017709618775828773 1107 78-79 0.3725898732789048 1107 80-81 0.5713279993090907 1107 82-83 0.4731057834142902 1107 84-85 0.7533440685034272 1107 86-87 0.7573157477580068 1107 88-89 0.6511380941092959 1107 90-91 0.7408907148583204 1107 92-93 0.7014869588249155 1107 94-95 0.6561465950648646 1107 96-97 0.9053684086454403 1107 98-99 0.969703283436079 1107 100-101 1.356307504507761 1108 1 0.24827865688827444 1108 2 0.0942550082552458 1108 3 0.14776122543164405 1108 4 0.2787139004238526 1108 5 0.324207898042701 1108 6 0.1428620217837988 1108 7 0.4200430355006688 1108 8 0.2966671589928467 1108 9 0.476698863238596 1108 10-11 0.4183788825307104 1108 12-13 0.1680793906034026 1108 14-15 0.3539687796991231 1108 16-17 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2316 86-87 -3.770203311773244 2316 88-89 -3.9283721652790113 2316 90-91 -4.064143681639489 2316 92-93 -4.286040588153849 2316 94-95 -4.06558238733485 2316 96-97 -4.020971342389437 2316 98-99 -4.632643049093179 2316 100-101 -4.664499770872684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 973.0 3 365.0 4 357.0 5 374.0 6 473.0 7 579.0 8 709.0 9 964.0 10 1309.0 11 2034.0 12 3054.0 13 4025.0 14 4838.0 15 6621.0 16 8812.0 17 11912.0 18 9656.0 19 5051.0 20 4809.0 21 5001.0 22 5290.0 23 5814.0 24 6730.0 25 7417.0 26 8622.0 27 10007.0 28 11952.0 29 14159.0 30 17113.0 31 20929.0 32 26116.0 33 33531.0 34 45109.0 35 60151.0 36 79228.0 37 95924.0 38 76762.0 39 12864.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.272479689202136 19.26260975705792 14.940576971033323 18.52433358270662 2 14.857649056325206 22.829629994176365 39.086919789692985 23.225801159805442 3 21.56924505427623 28.6311430900951 30.07954826950934 19.720063586119327 4 11.257302264522481 16.805382343288482 40.02215293731539 31.915162454873645 5 11.103379574849386 41.392365800862244 32.7867830116996 14.717471612588765 6 26.916599918589228 37.96089000354531 17.96125109970193 17.16125897816353 7 25.679520373305504 36.75152672632467 20.798426183889713 16.770526716480113 8 26.607730890583237 30.01688332783682 20.124631446305603 23.250754335274344 9 27.540288115600603 17.624563815423585 22.25964527755858 32.575502791417236 10-11 24.700434813647192 27.125101441474115 25.725639891587885 22.448823853290804 12-13 28.673037504574626 25.764557988246278 26.363239510354664 19.19916499682443 14-15 24.14648232095776 26.091332831288238 29.45019499365881 20.311989854095195 16-17 19.47687582445709 30.105645357117073 28.87224114508476 21.545237673341077 18-19 19.130057381393463 28.722775835164278 32.71067602402897 19.43649075941329 20-21 22.194092688545357 25.153490997273092 31.6864977144526 20.96591859972895 22-23 20.575658420905413 25.759356766640064 32.57435252987677 21.090632282577758 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27.518404545693137 29.977675056427483 21.325570180043044 50-51 19.395627964427284 28.320056034493362 31.386123753228425 20.89819224785093 52-53 20.769124146918987 29.520355429577194 28.185198034540708 21.525322388963115 54-55 19.738836451679575 27.735401513734832 29.503065854354695 23.0226961802309 56-57 21.647776530191273 27.5967957166865 28.24334443585077 22.512083317271454 58-59 23.05393887299546 27.053915907718956 28.324141754809585 21.568003464476 60-61 21.307203801653298 27.932129268678764 30.771886859149696 19.988780070518242 62-63 20.65924349967111 28.411903750350625 29.98000403528082 20.948848714697444 64-65 20.056593559816932 29.982037696232016 29.144863109200962 20.816505634750087 66-67 21.037958923558055 28.37573273164654 27.742058776007 22.8442495687884 68-69 20.40458772260076 28.371830930463176 29.010955841160396 22.212625505775666 70-71 22.11784063822143 28.302948476710593 28.109697179482552 21.46951370558542 72-73 21.180578338866944 27.318621146704114 30.142320966916447 21.3584795475125 74-75 19.710978913550395 30.830392242903038 28.435662501978726 21.022966341567837 76-77 20.213299082123346 28.371418940033315 28.717867960601268 22.697414017242075 78-79 20.470220464559112 28.538960772569517 29.003273738708064 21.98754502416331 80-81 21.5150453629239 28.729222845620033 27.985630220181847 21.77010157127422 82-83 20.79152699323252 27.84722603843627 29.56325104801212 21.797995920319092 84-85 20.267891563794446 28.85887708649469 28.880121396054626 21.993109953656237 86-87 20.15936941222789 29.727193687560494 28.030807591988054 22.082629308223563 88-89 19.843272864174462 29.15175579832167 27.01082440868479 23.994146928819077 90-91 20.306773326683487 27.49492238383825 28.18552739944023 24.01277689003803 92-93 21.53351073603528 28.491918442881143 28.50102372952541 21.473547091558167 94-95 20.37621977376616 27.898564692199106 28.88488255640467 22.840332977630066 96-97 18.4502992086718 28.171710345008595 30.53626264747182 22.84172779884778 98-99 19.560903296834915 29.197092268226033 27.997049936954298 23.24495449798475 100-101 18.995798733055437 27.794088686119405 29.140135228279778 24.069977352545376 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 313.0 1 258.0 2 286.5 3 368.5 4 331.5 5 348.0 6 560.0 7 1026.0 8 1655.5 9 2015.0 10 1838.5 11 1558.5 12 1508.0 13 1574.0 14 1685.5 15 1798.0 16 1846.0 17 1913.0 18 2033.5 19 2174.0 20 2319.0 21 2518.5 22 2786.0 23 3121.0 24 3532.0 25 3939.0 26 4622.5 27 5454.0 28 6118.5 29 7156.5 30 8469.5 31 9777.5 32 11712.0 33 13311.0 34 14444.0 35 15673.0 36 17818.0 37 19852.0 38 19463.0 39 18238.5 40 17090.5 41 16210.5 42 15483.5 43 15680.5 44 16361.0 45 17002.5 46 17935.5 47 25888.5 48 44030.0 49 42555.5 50 24578.5 51 16303.5 52 13376.0 53 12412.0 54 11736.5 55 10980.0 56 10394.0 57 9786.0 58 9481.5 59 9026.0 60 8212.0 61 7476.5 62 6413.5 63 4491.0 64 2810.5 65 2131.5 66 1588.5 67 1150.0 68 840.0 69 568.0 70 423.5 71 363.0 72 326.5 73 331.0 74 272.0 75 201.5 76 155.5 77 102.5 78 66.0 79 40.5 80 27.0 81 25.0 82 16.5 83 10.5 84 8.5 85 5.0 86 2.0 87 0.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017551485725065 2 0.008201628843488316 3 0.011154215227144111 4 0.038547655564395086 5 0.04888170790719037 6 0.062168346633641444 7 0.026409244876032377 8 0.025917147145423078 9 0.014434866764539438 10-11 0.048143561311276416 12-13 0.04855364275345084 14-15 0.020996169839330094 16-17 0.023456658492376586 18-19 0.03346264568143233 20-21 0.02509698426107425 22-23 0.014680915629844088 24-25 0.0026245212299162617 26-27 0.0021324234993069626 28-29 0.0010662117496534813 30-31 0.007135417093834835 32-33 0.0013942769033930139 34-35 0.0016403257686976635 36-37 0.007217433382269719 38-39 0.007463482247574369 40-41 0.004346863287048808 42-43 6.561303074790654E-4 44-45 0.0016403257686976635 46-47 0.0031986352489604434 48-49 4.92097730609299E-4 50-51 0.0017223420571325465 52-53 0.0010662117496534813 54-55 0.0027885538067860278 56-57 0.0022144397877418456 58-59 0.00311661896052556 60-61 9.021791727837149E-4 62-63 0.001968390922437196 64-65 0.004100814421744158 66-67 0.0025425049414813782 68-69 0.008447677708792966 70-71 0.010826150073404577 72-73 0.004428879575483691 74-75 0.004182830710179042 76-77 0.003526700402699976 78-79 0.03936781844874392 80-81 0.026491261164467265 82-83 0.00467492844078834 84-85 0.00984195461218598 86-87 0.007381465959139486 88-89 0.006643319363225536 90-91 0.015911159956367334 92-93 0.016239225110106868 94-95 0.007299449670704602 96-97 0.004428879575483691 98-99 0.02567109828011843 100-101 0.04838961017658107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 609635.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.268461095351 #Duplication Level Percentage of deduplicated Percentage of total 1 79.57808815427883 38.4111185211722 2 11.237571718702865 10.848405866208518 3 3.6974534798223746 5.354111683280294 4 1.6367276824230743 3.1600930585088864 5 0.9246170541518127 2.231492115321241 6 0.5595088198475385 1.6203977821989999 7 0.37283184594958385 1.259721361592777 8 0.2854193041924793 1.1021400464221471 9 0.2101467475708083 0.9129114098492498 >10 1.294929678924215 11.884590535481959 >50 0.12516045232912076 4.1391129769379305 >100 0.06632143444046172 5.892896436854497 >500 0.0027208793616599666 1.0102764840762914 >1k 0.007142308324357412 7.440323498749704 >5k 0.0010203297606224872 3.0321427788250084 >10k+ 3.401099202074958E-4 1.7002654445203362 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10357 1.6988853986401697 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6960 1.1416667350135736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6358 1.0429191237379742 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5152 0.8450958360330361 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4976 0.8162261025039573 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4359 0.7150180025753115 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3233 0.5303173210199545 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3187 0.5227718224839453 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2963 0.48602852526511764 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2828 0.4638841273876992 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2665 0.4371468173579273 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2557 0.4194312990559925 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2096 0.3438122811190302 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2034 0.3336422613531047 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1608 0.2637643836065843 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1550 0.25425049414813783 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1493 0.24490063726656117 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1457 0.23899546449924952 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1365 0.22390446742723108 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1259 0.2065170142790358 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1219 0.19995571120424516 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1165 0.1910979520532778 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1130 0.18535681186283595 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1095 0.17961567167239414 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1083 0.17764728074995695 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 937 0.15369852452697105 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 897 0.14713722145218042 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 894 0.1466451237215711 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 782 0.12827347511215728 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 726 0.11908765080745037 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 709 0.11629909700066433 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 638 0.10465278404291091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.6403257686976633E-4 0.0 5 0.0 0.0 0.0 1.6403257686976633E-4 0.0 6 0.0 0.0 0.0 1.6403257686976633E-4 0.0 7 0.0 0.0 0.0 1.6403257686976633E-4 0.0 8 0.0 0.0 0.0 1.6403257686976633E-4 0.0 9 0.0 0.0 0.0 1.6403257686976633E-4 0.0 10-11 0.0 0.0 0.0 1.6403257686976633E-4 0.0 12-13 0.0 0.0 0.0 1.6403257686976633E-4 0.0 14-15 0.0 0.0 0.0 1.6403257686976633E-4 0.0 16-17 0.0 0.0 0.0 2.460488653046495E-4 0.0 18-19 0.0 0.0 0.0 3.2806515373953266E-4 0.0 20-21 0.0 0.0 0.0 3.2806515373953266E-4 0.0 22-23 0.0 0.0 0.0 6.561303074790654E-4 0.0 24-25 0.0 0.0 0.0 0.0015583094802627802 0.0 26-27 0.0 0.0 0.0 0.002050407210872079 0.0 28-29 0.0 0.0 0.0 0.005331058748267406 0.0 30-31 0.0 0.0 0.0 0.012220426976797592 0.0 32-33 0.0 0.0 0.0 0.02124221870463474 0.0 34-35 0.0 0.0 0.0 0.027311424048816098 0.0 36-37 0.0 0.0 0.0 0.03149425475899514 0.0 38-39 0.0 0.0 0.0 0.036333215776653244 0.0 40-41 0.0 0.0 0.0 0.04428879575483691 0.0 42-43 0.0 0.0 0.0 0.053392603771108944 0.0 44-45 0.0 0.0 0.0 0.06175826519146703 0.0 46-47 0.0 0.0 0.0 0.07119013836147858 0.0 48-49 0.0 0.0 0.0 0.08365661420358084 0.0 50-51 0.0 0.0 0.0 0.10260237683203885 0.0 52-53 0.0 0.0 0.0 0.13426066416790375 0.0 54-55 0.0 0.0 0.0 0.18297833949822434 0.0 56-57 0.0 0.0 0.0 0.24145595315229607 0.0 58-59 0.0 0.0 0.0 0.3228161112797001 0.0 60-61 0.0 0.0 0.0 0.442969973836804 0.0 62-63 0.0 0.0 0.0 0.5220336758880313 0.0 64-65 0.0 0.0 0.0 0.5956022866141215 0.0 66-67 0.0 0.0 0.0 0.7694768180960738 0.0 68-69 0.0 0.0 0.0 0.975747783509805 0.0 70-71 0.0 0.0 0.0 1.1191942719824157 0.0 72-73 0.0 0.0 0.0 1.2464835516333546 0.0 74-75 0.0 0.0 0.0 1.5576533499553011 0.0 76-77 0.0 0.0 0.0 1.7357927284358672 0.0 78-79 0.0 0.0 0.0 1.8556185258392315 0.0 80-81 0.0 0.0 0.0 1.9982448514274935 0.0 82-83 0.0 0.0 0.0 2.263239479360601 0.0 84-85 0.0 0.0 0.0 2.5494763259983433 0.0 86-87 0.0 0.0 0.0 2.911742272015222 0.0 88-89 0.0 0.0 0.0 3.5167764317993555 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGT 385 0.0 30.85311 4 GATAGTC 85 6.3897704E-4 27.95158 7 GTACATG 7020 0.0 27.411802 1 TTACGCC 35 0.008389962 27.144053 38-39 GGCGCGA 35 0.008389962 27.144053 20-21 TACATGG 7075 0.0 26.59429 2 CGGTATA 55 9.474252E-5 25.91023 34-35 ACATGGG 6935 0.0 25.349823 3 CATATAG 170 1.1178781E-7 25.154358 3 GAGTACT 4855 0.0 24.564287 12-13 AGTACTT 4975 0.0 23.542007 12-13 CATGGGA 5375 0.0 23.425402 4 CATGGGG 1245 0.0 23.279848 4 CCTATAC 205 2.6559064E-8 23.177458 3 ATGGGAT 1895 0.0 23.067379 5 ACTTGAC 145 2.3253126E-5 22.939573 8 GGTACTC 125 2.2485375E-4 22.808489 8 GTACTTT 5325 0.0 22.260502 14-15 CTATACT 195 4.202957E-7 21.929441 4 GTATATG 155 3.8938193E-5 21.45784 1 >>END_MODULE