##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936562_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609635 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55565379284326 31.0 31.0 34.0 30.0 34.0 2 31.742437688124863 31.0 31.0 34.0 30.0 34.0 3 31.989377250321915 33.0 31.0 34.0 30.0 34.0 4 35.803203556226265 37.0 35.0 37.0 35.0 37.0 5 35.67967062258565 37.0 35.0 37.0 33.0 37.0 6 35.84082606805712 37.0 35.0 37.0 35.0 37.0 7 35.78534040860515 37.0 35.0 37.0 35.0 37.0 8 35.79040901523042 37.0 35.0 37.0 33.0 37.0 9 37.39314179796108 39.0 37.0 39.0 34.0 39.0 10-11 37.278323094966666 39.0 37.0 39.0 34.0 39.0 12-13 37.16630196757076 39.0 37.0 39.0 33.0 39.0 14-15 38.10277953201506 40.0 37.0 41.0 33.0 41.0 16-17 38.103127281078024 40.0 37.5 41.0 33.0 41.0 18-19 38.336579264641955 40.0 38.0 41.0 33.5 41.0 20-21 38.47074643024105 40.0 38.0 41.0 34.0 41.0 22-23 38.381389683991245 40.0 38.0 41.0 34.0 41.0 24-25 38.23775783870677 40.0 37.5 41.0 33.5 41.0 26-27 37.98285285457692 40.0 37.0 41.0 33.0 41.0 28-29 37.87359485593839 40.0 37.0 41.0 33.0 41.0 30-31 37.72956113084059 39.0 37.0 41.0 33.0 41.0 32-33 37.37760955325727 39.0 35.5 41.0 33.0 41.0 34-35 37.08775414797378 39.0 35.0 41.0 32.0 41.0 36-37 36.766206008513294 39.0 35.0 41.0 31.5 41.0 38-39 36.53170503662027 39.0 35.0 41.0 31.0 41.0 40-41 36.26385870233828 39.0 35.0 40.5 30.5 41.0 42-43 35.827057993717546 38.5 35.0 40.0 30.0 41.0 44-45 35.25074593814331 38.0 35.0 40.0 27.0 41.0 46-47 34.43949248320716 38.0 34.5 40.0 22.5 41.0 48-49 33.61762940119908 38.0 33.0 40.0 13.5 41.0 50-51 33.31219500192738 38.0 33.0 40.0 7.5 41.0 52-53 33.081115749587866 38.0 33.0 40.0 4.0 41.0 54-55 32.82966775201555 38.0 33.0 40.0 2.0 41.0 56-57 32.47989534721596 37.5 32.5 40.0 2.0 41.0 58-59 32.11693882405046 37.0 31.5 40.0 2.0 41.0 60-61 31.64009120211274 36.0 31.0 39.0 2.0 41.0 62-63 31.42828905820696 36.0 31.0 39.0 2.0 40.0 64-65 31.033397852813568 35.0 30.5 39.0 2.0 40.0 66-67 30.575380350537614 35.0 30.0 38.0 2.0 40.0 68-69 30.101690355704644 35.0 29.0 38.0 2.0 40.0 70-71 29.563466664479563 34.0 29.0 37.0 2.0 39.0 72-73 29.130398517145505 34.0 28.5 36.5 2.0 39.0 74-75 28.80493902088955 34.0 28.0 36.0 2.0 39.0 76-77 27.452147596512667 32.5 26.0 34.5 2.0 36.5 78-79 28.188992593929154 34.0 28.0 35.0 2.0 37.0 80-81 27.94746938742034 34.0 27.0 35.0 2.0 37.0 82-83 27.600125484921307 33.5 26.5 35.0 2.0 36.5 84-85 27.146489292773545 33.0 26.0 35.0 2.0 36.0 86-87 26.858308660099894 33.0 25.5 35.0 2.0 36.0 88-89 26.43695489924299 33.0 25.0 35.0 2.0 35.0 90-91 25.924173480853298 33.0 23.5 35.0 2.0 35.0 92-93 25.52458766310989 32.0 21.0 34.5 2.0 35.0 94-95 24.994613990338483 32.0 18.5 34.0 2.0 35.0 96-97 24.309153837952216 32.0 6.0 34.0 2.0 35.0 98-99 23.599540708784765 31.0 2.0 34.0 2.0 35.0 100-101 21.025751474242785 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27698259043333806 1101 2 0.28800304332440874 1101 3 0.1911183223120254 1101 4 0.09912142358378162 1101 5 0.15638152595618493 1101 6 0.053505341183949895 1101 7 0.21799574252057852 1101 8 0.09223212015553628 1101 9 0.3660893047062146 1101 10-11 0.22658182625477963 1101 12-13 0.1341316153488421 1101 14-15 0.2663978724927034 1101 16-17 0.28698932160050816 1101 18-19 0.28846864956876317 1101 20-21 -0.06373922637701668 1101 22-23 0.019587393748800253 1101 24-25 -0.138616878718544 1101 26-27 0.1937764032038345 1101 28-29 0.06054990673041516 1101 30-31 -0.07158358395514597 1101 32-33 0.07794727005056501 1101 34-35 0.21107921766867577 1101 36-37 0.14398077689170918 1101 38-39 0.26691568654326403 1101 40-41 0.3386913045125013 1101 42-43 0.3898084324387199 1101 44-45 0.31847510256064737 1101 46-47 0.5302054563486109 1101 48-49 0.4583160846956744 1101 50-51 0.19611802734798545 1101 52-53 0.0660863709856514 1101 54-55 0.27373862998597076 1101 56-57 0.41977648557863034 1101 58-59 0.004761366281620383 1101 60-61 0.23647864485548453 1101 62-63 0.1764916121700928 1101 64-65 -0.053915473233313804 1101 66-67 -0.05576201548511506 1101 68-69 -0.2877236773129326 1101 70-71 -0.3114884749858575 1101 72-73 -0.22430631293845238 1101 74-75 -0.0681672216065543 1101 76-77 0.087383086945092 1101 78-79 0.032364093193692156 1101 80-81 -0.06647698129207669 1101 82-83 -0.13906675014411007 1101 84-85 0.2758022603468788 1101 86-87 0.3320805327344125 1101 88-89 0.40851737882175954 1101 90-91 0.3451656689845919 1101 92-93 0.4331076734871644 1101 94-95 0.4568595124190651 1101 96-97 0.6466904402228053 1101 98-99 0.23846138360901037 1101 100-101 0.5325187273282204 1102 1 0.3665070045243404 1102 2 0.45808994464653807 1102 3 0.14760381686883406 1102 4 0.08565208347929598 1102 5 0.2048923101840714 1102 6 0.18669019073011128 1102 7 0.190615624025412 1102 8 0.05678572024893214 1102 9 0.14193533395059177 1102 10-11 0.1558221875774919 1102 12-13 0.12551627226738304 1102 14-15 0.2687373129970396 1102 16-17 0.3251992711087226 1102 18-19 0.14014447334368185 1102 20-21 0.10351663271296019 1102 22-23 0.13223806648615977 1102 24-25 0.2091872394263916 1102 26-27 0.03234497571645534 1102 28-29 0.16072903582010412 1102 30-31 0.2981638476766406 1102 32-33 0.35793497544705843 1102 34-35 0.3619544329154465 1102 36-37 0.2080376677068756 1102 38-39 0.31913491720024467 1102 40-41 0.4579608153551007 1102 42-43 0.3204342168125365 1102 44-45 0.43103912935340105 1102 46-47 0.563370787755666 1102 48-49 0.6362284829094946 1102 50-51 0.17705865572261104 1102 52-53 0.06818776315795816 1102 54-55 0.1602242339902915 1102 56-57 0.14311872064268982 1102 58-59 0.1886578070486351 1102 60-61 0.4142724749345774 1102 62-63 0.2507435384462511 1102 64-65 0.33532455974686926 1102 66-67 0.2854798124342146 1102 68-69 0.4531766743783976 1102 70-71 0.3760926985832995 1102 72-73 0.16305487113766404 1102 74-75 0.08770883273778196 1102 76-77 0.3533742800688664 1102 78-79 0.35074689680107696 1102 80-81 0.18917871873419045 1102 82-83 0.4826115179585102 1102 84-85 0.6446868807829169 1102 86-87 0.763388539914164 1102 88-89 0.8070750430659857 1102 90-91 0.7787574808516204 1102 92-93 0.9328440874030477 1102 94-95 1.0166798725743362 1102 96-97 1.1654132607580792 1102 98-99 0.7520164575955235 1102 100-101 -0.07355962249520687 1103 1 0.5585340549588196 1103 2 0.4209298794021592 1103 3 0.300623173663201 1103 4 0.03670436558905266 1103 5 -0.007040049295127915 1103 6 -0.045412713510778246 1103 7 0.06893558129900157 1103 8 0.26206012193488704 1103 9 0.10047015019816996 1103 10-11 0.03021052153632553 1103 12-13 0.12791782831692444 1103 14-15 0.15239716893976407 1103 16-17 0.22610035081657287 1103 18-19 0.3110149156197295 1103 20-21 0.19808669824603697 1103 22-23 -0.01644549828187536 1103 24-25 -0.036181059312028196 1103 26-27 -0.11538951430923561 1103 28-29 0.012126665659302205 1103 30-31 -0.06619153931490018 1103 32-33 -0.007244987889073684 1103 34-35 -0.0889228297076059 1103 36-37 0.00572145621116249 1103 38-39 0.10861534388693173 1103 40-41 0.30957947495843285 1103 42-43 0.28312709911283207 1103 44-45 0.5059970885900924 1103 46-47 0.46531386492302573 1103 48-49 0.1520109102313043 1103 50-51 0.014172349824640662 1103 52-53 0.17260763671089308 1103 54-55 0.382115563127968 1103 56-57 0.6195361980525433 1103 58-59 0.27579676169512624 1103 60-61 0.4408682196154281 1103 62-63 0.13683021098298553 1103 64-65 0.19000335388065182 1103 66-67 0.13994389193997492 1103 68-69 0.21745339281715204 1103 70-71 0.312397333532342 1103 72-73 0.48352006210738097 1103 74-75 0.10599656477392827 1103 76-77 0.20659157127242267 1103 78-79 0.17541706626324682 1103 80-81 0.14427900309575037 1103 82-83 0.23843407345953693 1103 84-85 0.3531847083760944 1103 86-87 0.23835443817912605 1103 88-89 0.12379806480448963 1103 90-91 -0.1679905849124026 1103 92-93 -0.19196375202649563 1103 94-95 0.07725826686978365 1103 96-97 -0.10464361155347746 1103 98-99 0.18215481320625315 1103 100-101 0.1213236737893304 1104 1 0.19464098957321951 1104 2 0.2757797088788543 1104 3 0.10627978006607819 1104 4 -0.05577977391001099 1104 5 -0.07585812109827827 1104 6 -0.05932044756776378 1104 7 -0.19525269109822574 1104 8 -0.06776862132668526 1104 9 -0.13380503407701383 1104 10-11 -0.12223272904125082 1104 12-13 0.047159458006312605 1104 14-15 0.22725873335355828 1104 16-17 -0.01835305781594343 1104 18-19 -0.03479442832394142 1104 20-21 -0.0714042988568977 1104 22-23 -0.10838873052362175 1104 24-25 -0.022662961838705087 1104 26-27 0.1473392252851724 1104 28-29 -0.10415739316207606 1104 30-31 0.010969339338515738 1104 32-33 -0.12207868182724013 1104 34-35 -0.0374202076826009 1104 36-37 -0.2485645462091668 1104 38-39 -0.4452195968883146 1104 40-41 -0.18174950666905687 1104 42-43 -0.2942904812898277 1104 44-45 3.569844425257429E-4 1104 46-47 -0.10975672805802361 1104 48-49 -0.030656220943292567 1104 50-51 -0.12069508205771484 1104 52-53 -0.10442580785986166 1104 54-55 -0.15014451241569304 1104 56-57 -0.45810324600481067 1104 58-59 -0.2659676961141031 1104 60-61 -0.35575340200619365 1104 62-63 -0.6435311884082608 1104 64-65 -0.7105962280622187 1104 66-67 -0.4895309696244432 1104 68-69 -0.5584212267141844 1104 70-71 -0.4650624193005406 1104 72-73 -0.3833690794086273 1104 74-75 -0.4743419148182859 1104 76-77 -0.31262988899381483 1104 78-79 -0.06489427628689981 1104 80-81 -0.4171359736177358 1104 82-83 -0.23815472700986362 1104 84-85 -0.1787856609077103 1104 86-87 -0.03182782006283347 1104 88-89 -0.1367899655596574 1104 90-91 -0.33070557262739086 1104 92-93 -0.10565145601270842 1104 94-95 0.03643260402919424 1104 96-97 -0.20475637696475246 1104 98-99 0.04311047714698901 1104 100-101 0.13428252816609643 1105 1 0.25475611174757873 1105 2 0.3233800595821634 1105 3 0.1124685499360858 1105 4 0.042003552434671576 1105 5 -0.024007398725693463 1105 6 0.04963582887793194 1105 7 0.01721061847369043 1105 8 0.24468897025643344 1105 9 0.21151002710546862 1105 10-11 0.10008553421494781 1105 12-13 0.081520758658165 1105 14-15 0.13776077276956755 1105 16-17 0.14832631549248276 1105 18-19 0.13149898074097877 1105 20-21 0.10069763382330876 1105 22-23 -0.03804222040277949 1105 24-25 0.052161686504980764 1105 26-27 0.022250596458881944 1105 28-29 0.07390649583270914 1105 30-31 0.21694273163066669 1105 32-33 0.22987496434361532 1105 34-35 0.06616211229604829 1105 36-37 0.17041631758834086 1105 38-39 0.13774556149997608 1105 40-41 -0.1745124388537036 1105 42-43 0.07889215127222116 1105 44-45 -0.022511158735909476 1105 46-47 -0.106871049713952 1105 48-49 -0.22769620874492347 1105 50-51 -0.19529958783288492 1105 52-53 -0.3136956206910284 1105 54-55 -0.19619719415243253 1105 56-57 -0.2703607079801813 1105 58-59 -0.15233696383034 1105 60-61 -0.25870864921391146 1105 62-63 -0.18870425286905856 1105 64-65 -0.18920523198856998 1105 66-67 -0.21561834049319373 1105 68-69 -0.4644355979260162 1105 70-71 -0.3157333299855587 1105 72-73 -0.3112881126873184 1105 74-75 0.14812787514092918 1105 76-77 -0.01683642274900521 1105 78-79 0.07492389166948854 1105 80-81 0.15909720596523513 1105 82-83 0.14789338033584798 1105 84-85 0.32188524977730637 1105 86-87 0.03650976999549371 1105 88-89 0.08001996849286996 1105 90-91 -0.13792262748196293 1105 92-93 0.25989766151102245 1105 94-95 0.21731832049319166 1105 96-97 -0.025971068383956464 1105 98-99 0.22540758384466386 1105 100-101 0.5771264651919772 1106 1 0.24959334076834594 1106 2 0.20151787479042937 1106 3 0.048038880206679124 1106 4 -0.06073212510513315 1106 5 -0.22397560274176698 1106 6 -0.14024530305909622 1106 7 -0.05463403776280984 1106 8 -0.0542707303721528 1106 9 -0.0896244372952637 1106 10-11 -0.17481826255164634 1106 12-13 -0.1250909716140569 1106 14-15 -0.10188515041459567 1106 16-17 -0.06156896158098135 1106 18-19 -0.07556930724911126 1106 20-21 -0.06296811701032112 1106 22-23 -0.018512148739525003 1106 24-25 -0.2884222182049001 1106 26-27 -0.013928545375662793 1106 28-29 -0.12117817116550356 1106 30-31 -0.23091083563414827 1106 32-33 -0.2006640591120359 1106 34-35 -0.39915274947293966 1106 36-37 -0.13217648184422615 1106 38-39 0.02489835343195068 1106 40-41 -0.14525911454281726 1106 42-43 0.08852470383749989 1106 44-45 0.26409702506118293 1106 46-47 0.27569476389636094 1106 48-49 0.06256749410123064 1106 50-51 0.315514104006958 1106 52-53 0.42415566378518577 1106 54-55 0.1575300696808597 1106 56-57 0.23196080648698825 1106 58-59 0.1921052612363141 1106 60-61 0.22699538724548418 1106 62-63 -0.25477161959089045 1106 64-65 -0.001246126515582091 1106 66-67 0.1149019314847628 1106 68-69 0.29362142287181925 1106 70-71 0.3243962590309657 1106 72-73 0.6547187193728128 1106 74-75 0.6374374946489887 1106 76-77 0.5293635495314177 1106 78-79 0.4484747285560928 1106 80-81 0.31365218448256016 1106 82-83 0.5782255260755917 1106 84-85 0.3371024815700281 1106 86-87 0.6220582914822401 1106 88-89 0.5609760085068913 1106 90-91 0.12628161687424821 1106 92-93 0.5449343899488603 1106 94-95 0.6027855176364412 1106 96-97 0.17317675848283187 1106 98-99 0.05916656213371141 1106 100-101 -0.2621203776298735 1107 1 0.5981195150029635 1107 2 0.572818398600468 1107 3 0.3406974059471146 1107 4 0.08698935544448716 1107 5 0.20295126990623658 1107 6 0.2169459689425537 1107 7 0.11200099786277917 1107 8 0.08191445762468419 1107 9 0.19001475193789474 1107 10-11 0.3734457355396543 1107 12-13 0.2691208189920644 1107 14-15 0.44126410833454344 1107 16-17 0.46042590876789546 1107 18-19 0.41350307180282897 1107 20-21 0.24552942661340893 1107 22-23 0.25481800341481886 1107 24-25 0.24199780587083808 1107 26-27 0.3564488039069502 1107 28-29 0.481946252697675 1107 30-31 0.42492400695171284 1107 32-33 0.3772418394445012 1107 34-35 0.37709604402876806 1107 36-37 0.6233042738303851 1107 38-39 0.49693470563184405 1107 40-41 0.7198503107844232 1107 42-43 0.6136198680839371 1107 44-45 0.31802343137727007 1107 46-47 0.6164437688741842 1107 48-49 0.7914293730935213 1107 50-51 0.5939644179676336 1107 52-53 0.5615812404642568 1107 54-55 0.43995233865676653 1107 56-57 0.7786672017412499 1107 58-59 0.745004110589413 1107 60-61 0.9371158743996695 1107 62-63 0.7930884049186488 1107 64-65 0.6302865872108434 1107 66-67 0.8757319480775045 1107 68-69 0.7127833268074397 1107 70-71 0.8082224039842814 1107 72-73 0.8200333849694488 1107 74-75 1.0190027431605202 1107 76-77 0.6976307468765803 1107 78-79 0.8423340589902146 1107 80-81 1.1431563543575187 1107 82-83 1.1867425905703826 1107 84-85 1.0225073769706938 1107 86-87 0.8754486872136766 1107 88-89 0.8213396931797554 1107 90-91 0.7228502815382178 1107 92-93 1.0271080389419112 1107 94-95 1.2523940157223166 1107 96-97 1.081424597630857 1107 98-99 1.0615375345984397 1107 100-101 1.2093357709022676 1108 1 0.3421354591317467 1108 2 0.4173397742823326 1108 3 0.16626342919563442 1108 4 0.04957273162042952 1108 5 -0.18085956382213908 1108 6 -0.07266564329729874 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-0.4899359281709401 2316 88-89 -0.7934716472413399 2316 90-91 -1.0355050149268337 2316 92-93 -1.34405771941897 2316 94-95 -1.771841741745149 2316 96-97 -1.0689670161753924 2316 98-99 -1.8535433195068052 2316 100-101 -3.171246214862645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 1.0 5 27.0 6 49.0 7 129.0 8 322.0 9 620.0 10 1045.0 11 1997.0 12 2964.0 13 4063.0 14 5548.0 15 7943.0 16 11208.0 17 15197.0 18 12260.0 19 5895.0 20 4446.0 21 4428.0 22 4417.0 23 4814.0 24 5417.0 25 6427.0 26 7698.0 27 9171.0 28 11392.0 29 13779.0 30 17079.0 31 21196.0 32 26638.0 33 33490.0 34 44880.0 35 61779.0 36 85150.0 37 106752.0 38 66786.0 39 4624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.16216261063783 19.792347695646836 15.909777280707285 17.135712413008054 2 15.607640996531892 24.205402638650554 38.13854063790903 22.048415726908527 3 22.324492621439493 28.470777750802164 30.707147590895072 18.497582036863275 4 11.8577749486909 17.879683237058703 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21.54174218999893 25.210658836844996 34.60956145890574 18.638037514250332 26-27 20.682703584931968 23.943261131660748 36.92151861359666 18.452516669810628 28-29 18.794196527430348 27.297809344935903 35.38658377553782 18.521410352095927 30-31 19.59731609677602 25.28605254459844 34.72351056247637 20.393120796149166 32-33 19.493212402467503 25.936980452079993 34.93510956694053 19.634697578511968 34-35 21.411441656605952 24.70506797543436 34.80854023410845 19.07495013385124 36-37 20.889499477126865 24.16961594455494 35.85343148311427 19.08745309520392 38-39 19.86961039770551 25.86558011398633 35.51882316371531 18.74598632459285 40-41 19.742062271521945 26.553035615714784 34.47866608596229 19.226236026800983 42-43 19.23609772502635 27.100526192751417 32.51731760633564 21.14605847588659 44-45 20.73451095893894 25.026717616731176 32.687107386365035 21.55166403796485 46-47 22.26899026947581 27.102675730117383 31.638802679666945 18.98953132073986 48-49 21.847296135649273 27.018383390313204 30.868871929813047 20.265448544224473 50-51 19.681183930265956 27.967104664218734 32.3736263195493 19.978085085966008 52-53 21.110130539908337 29.482695834467915 28.83016032885952 20.577013296764225 54-55 19.955902907652025 27.712102420746632 29.897762991252762 22.434231680348578 56-57 22.036456214485376 27.23799556436267 29.04907743992861 21.676470781223344 58-59 23.762684006259576 26.689806537122763 28.630832674459423 20.91667678215824 60-61 21.374085247321563 27.8409622189304 31.42967990012976 19.355272633618277 62-63 20.996689675988502 27.86479180063254 30.81939541475834 20.31912310862062 64-65 20.38495834020349 29.87184188226647 29.73140361973059 20.01179615779945 66-67 21.668073295620914 27.770792477528794 28.475747338272527 22.085386888577762 68-69 21.133892520846505 28.142436164619948 29.14254360604592 21.581127708487628 70-71 23.015087988661865 28.278250200123356 28.436954581840613 20.26970722937417 72-73 22.182471628492152 26.943357044261766 30.485069332526294 20.389101994719788 74-75 20.418365087306338 30.789652825051057 28.589483871496878 20.202498216145727 76-77 21.113026675371458 28.36747448435598 28.714326488699626 21.80517235157293 78-79 21.0441353310683 28.50988504766576 29.081201593944456 21.36477802732148 80-81 22.089124951295037 28.536964501773888 28.375694686545128 20.998215860385947 82-83 21.564115940887735 27.80599522272095 29.625938394099272 21.003950442292044 84-85 20.57718618454078 28.828140123167174 29.197001860303356 21.39767183198869 86-87 20.545630526436668 29.58191840149614 27.836835802286856 22.035615269780337 88-89 20.111526969442888 29.41895056026017 27.019898301580607 23.449624168716333 90-91 20.915234826210227 27.4398987976478 28.11384280163814 23.53102357450383 92-93 21.92650783444004 28.605876486181465 28.495151045904304 20.972464633474186 94-95 21.043721194235033 27.833822879251812 28.730508047773995 22.391947878739163 96-97 19.114454100653333 28.309088000918685 30.522870723914906 22.053587174513076 98-99 20.311233746846156 29.344591468796246 27.59952032074859 22.744654463609013 100-101 19.257144169580005 28.640905712874844 28.276048186129838 23.825901931415316 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 771.0 1 564.5 2 407.5 3 464.5 4 422.5 5 426.5 6 646.0 7 1204.0 8 1930.0 9 2307.5 10 2136.0 11 1849.0 12 1832.5 13 1951.0 14 2072.0 15 2151.0 16 2251.0 17 2368.5 18 2488.5 19 2621.0 20 2734.5 21 2912.0 22 3254.0 23 3552.5 24 3845.5 25 4261.0 26 4923.0 27 5846.5 28 6557.5 29 7477.0 30 8789.5 31 9980.0 32 11609.5 33 13250.5 34 14202.0 35 15305.0 36 17550.5 37 19189.0 38 18811.5 39 17792.5 40 16846.5 41 15976.0 42 14992.0 43 15298.5 44 16128.5 45 16330.0 46 16979.0 47 26252.0 48 46006.5 49 43189.0 50 23295.0 51 15178.0 52 12582.5 53 11789.0 54 11195.5 55 10601.0 56 10112.0 57 9544.0 58 9533.5 59 9101.5 60 7910.0 61 6807.5 62 5701.5 63 4162.0 64 2740.5 65 2051.5 66 1535.5 67 1165.5 68 875.5 69 589.5 70 463.5 71 402.0 72 361.5 73 400.5 74 331.0 75 258.0 76 218.0 77 141.5 78 86.0 79 58.0 80 43.0 81 35.0 82 25.0 83 10.0 84 5.5 85 3.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.147079810050276 2 0.013286638726451073 3 0.0050850098829627564 4 0.015419062225758036 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003280651537395327 10-11 2.460488653046495E-4 12-13 2.460488653046495E-4 14-15 8.201628843488318E-5 16-17 0.0 18-19 0.0 20-21 8.201628843488318E-5 22-23 0.0017223420571325465 24-25 0.0 26-27 0.0 28-29 0.0 30-31 8.201628843488318E-5 32-33 0.005002993594527873 34-35 4.92097730609299E-4 36-37 0.003690732979569743 38-39 8.201628843488318E-5 40-41 0.005002993594527873 42-43 0.011072198938709227 44-45 0.0030346026720906773 46-47 0.0011482280380883643 48-49 0.01033405234279528 50-51 7.381465959139486E-4 52-53 0.0021324234993069626 54-55 0.01164631295775341 56-57 0.004428879575483691 58-59 0.0014762931918278971 60-61 0.007217433382269719 62-63 0.004428879575483691 64-65 0.018781730051588244 66-67 0.003526700402699976 68-69 8.201628843488318E-5 70-71 0.0018043583455674298 72-73 8.201628843488318E-5 74-75 0.0 76-77 0.0014762931918278971 78-79 0.012794540995841774 80-81 0.015993176244802218 82-83 0.013614703880190607 84-85 0.00934985688157668 86-87 0.010662117496534812 88-89 0.03051005929777654 90-91 0.026737310029771914 92-93 0.00311661896052556 94-95 0.009267840593141797 96-97 0.011072198938709227 98-99 0.010005987189055746 100-101 0.013450671303320842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 609635.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.22114766392175 #Duplication Level Percentage of deduplicated Percentage of total 1 78.09442036540858 35.315193150725186 2 11.952222666232279 10.809864522035248 3 3.9709892220761134 5.3871806995003695 4 1.800994012669438 3.257720647550544 5 1.0072002611642459 2.277337586862445 6 0.6109602647191507 1.657699460859206 7 0.4170689437951421 1.32022354053772 8 0.2896871169934736 1.0479987115118117 9 0.2164211775474497 0.8808132624725742 >10 1.4036896535601404 12.05417046815384 >50 0.15013653154354614 4.707280072348807 >100 0.07159795644463615 6.089927946648663 >500 0.00511413974604544 1.5442045908227553 >1k 0.00767120961906816 7.337780051812878 >5k 0.0014611827845844114 4.128451126855189 >10k+ 3.6529569614610286E-4 2.184154161302789 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13222 2.1688387313720505 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7840 1.286015402658968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6707 1.1001664930655228 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5330 0.8742936347158546 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5115 0.8390266306888547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4246 0.6964823213890279 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3549 0.5821516153108007 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3450 0.5659123902006938 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3373 0.5532818817817219 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3293 0.5401592756321405 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3275 0.5372066892484847 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2718 0.4458405439320249 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2691 0.4414116643565412 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2146 0.3520139099625186 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1992 0.32675289312457456 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1756 0.2880412049833097 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1447 0.23735513873055192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1365 0.22390446742723108 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1316 0.2158668711606125 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1271 0.20848540520147305 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1123 0.1842085838247476 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1111 0.18224019290231042 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1107 0.18158406259483134 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1096 0.1797797042492639 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1068 0.17518679209691043 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1027 0.16846145644525 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 944 0.15484675256505942 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 908 0.14894157979774783 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 843 0.13827946230121302 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 740 0.1213841068836271 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 728 0.1194157159611899 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 690 0.11318247804013876 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 8.201628843488316E-5 0.0 18-19 0.0 0.0 0.0 1.6403257686976633E-4 0.0 20-21 0.0 0.0 0.0 1.6403257686976633E-4 0.0 22-23 0.0 0.0 0.0 4.92097730609299E-4 0.0 24-25 0.0 0.0 0.0 0.0013942769033930139 0.0 26-27 0.0 0.0 0.0 0.002050407210872079 0.0 28-29 0.0 0.0 0.0 0.005331058748267406 0.0 30-31 0.0 0.0 0.0 0.012056394399927825 0.0 32-33 0.0 0.0 0.0 0.021324234993069624 0.0 34-35 0.0 0.0 0.0 0.027557472914120744 0.0 36-37 0.0 0.0 0.0 0.0316582873358649 0.0 38-39 0.0 0.0 0.0 0.03657926464195789 0.0 40-41 0.0 0.0 0.0 0.044124763177967145 0.0 42-43 0.0 0.0 0.0 0.053392603771108944 0.0 44-45 0.0 0.0 0.0 0.06208633034520655 0.0 46-47 0.0 0.0 0.0 0.07135417093834837 0.0 48-49 0.0 8.201628843488316E-5 0.0 0.08373863049201571 0.0 50-51 0.0 1.6403257686976633E-4 0.0 0.10252036054360396 0.0 52-53 0.0 1.6403257686976633E-4 0.0 0.13458872932164329 0.0 54-55 0.0 1.6403257686976633E-4 0.0 0.1837164860941383 0.0 56-57 0.0 1.6403257686976633E-4 0.0 0.24161998572916582 0.0 58-59 0.0 1.6403257686976633E-4 0.0 0.3232261927218746 0.0 60-61 0.0 1.6403257686976633E-4 0.0 0.44436425074019703 0.0 62-63 0.0 1.6403257686976633E-4 0.0 0.5252323111369919 0.0 64-65 0.0 1.6403257686976633E-4 0.0 0.5999491499011704 0.0 66-67 0.0 1.6403257686976633E-4 0.0 0.7742337628252971 0.0 68-69 0.0 1.6403257686976633E-4 0.0 0.9817349725655515 0.0 70-71 0.0 1.6403257686976633E-4 0.0 1.1270678356721646 0.0 72-73 0.0 1.6403257686976633E-4 0.0 1.2555873596496263 0.0 74-75 0.0 1.6403257686976633E-4 0.0 1.568233451163401 0.0 76-77 0.0 1.6403257686976633E-4 0.0 1.7469469436630116 0.0 78-79 0.0 1.6403257686976633E-4 0.0 1.8662806433357666 0.0 80-81 0.0 1.6403257686976633E-4 0.0 2.008988985212463 0.0 82-83 0.0 1.6403257686976633E-4 0.0 2.2790686230285333 0.0 84-85 0.0 1.6403257686976633E-4 0.0 2.567191844300278 0.0 86-87 0.0 1.6403257686976633E-4 0.0 2.928063513413764 0.0 88-89 0.0 1.6403257686976633E-4 0.0 3.5375265527733806 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTAC 55 0.0025804376 34.540653 5 TAGGCCC 65 0.005848261 29.224306 4 CTGGTCG 245 0.0 29.077589 9 GGGTACC 140 5.805632E-7 27.139084 7 ATAGACC 70 0.008397583 27.136858 3 CATGGGT 390 0.0 25.571268 4 GTATAGG 310 0.0 24.793402 1 GAGTACT 6635 0.0 24.512163 12-13 GTCGGGA 235 1.7644197E-10 24.25195 2 CGCGCGT 110 2.1700544E-9 23.748646 32-33 ACACGCT 140 1.7796165E-5 23.746698 9 CATATAG 140 1.7807468E-5 23.74475 3 ATAGGGG 140 1.7807468E-5 23.74475 3 GTACTTT 6945 0.0 23.349653 14-15 CGGGTCG 165 2.4297042E-6 23.027102 5 TACCTGG 335 0.0 22.683413 2 GTACATG 6695 0.0 22.601526 1 AGTACTT 6815 0.0 22.43634 12-13 TACATGG 6865 0.0 21.999853 2 TCGAGGT 55 0.00288253 21.584366 14-15 >>END_MODULE