##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936560_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 662284 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937214246456204 31.0 31.0 34.0 30.0 34.0 2 32.04721690392641 31.0 31.0 34.0 30.0 34.0 3 32.09734494567285 33.0 31.0 34.0 30.0 34.0 4 35.826965471006396 37.0 35.0 37.0 35.0 37.0 5 35.70635407166714 37.0 35.0 37.0 33.0 37.0 6 35.84954943800545 37.0 35.0 37.0 35.0 37.0 7 35.79808511152315 37.0 35.0 37.0 35.0 37.0 8 35.78646773891563 37.0 35.0 37.0 33.0 37.0 9 37.374152176407705 39.0 37.0 39.0 34.0 39.0 10-11 37.26670355919817 39.0 37.0 39.0 34.0 39.0 12-13 37.174906837550054 39.0 37.0 39.0 33.0 39.0 14-15 38.11769875159297 40.0 37.0 41.0 33.0 41.0 16-17 38.1025926943728 40.0 37.0 41.0 33.0 41.0 18-19 38.4102990559941 40.0 38.0 41.0 34.0 41.0 20-21 38.487525744242646 40.0 38.0 41.0 34.0 41.0 22-23 38.39109279402794 40.0 38.0 41.0 34.0 41.0 24-25 38.218069589481246 40.0 37.5 41.0 33.5 41.0 26-27 37.97209882769326 40.0 37.0 41.0 33.0 41.0 28-29 37.86267824679443 40.0 37.0 41.0 33.0 41.0 30-31 37.7170669984478 39.0 37.0 41.0 33.0 41.0 32-33 37.37771258251747 39.0 35.5 41.0 33.0 41.0 34-35 37.077367866353406 39.0 35.0 41.0 32.0 41.0 36-37 36.775748017466825 39.0 35.0 41.0 31.5 41.0 38-39 36.5389915806512 39.0 35.0 40.5 31.0 41.0 40-41 36.31716151983137 39.0 35.0 40.0 30.5 41.0 42-43 35.85495497399907 38.5 35.0 40.0 30.0 41.0 44-45 35.2839476719957 38.0 35.0 40.0 27.0 41.0 46-47 34.499624028362454 38.0 34.5 40.0 23.0 41.0 48-49 33.77692500498276 38.0 33.5 40.0 16.5 41.0 50-51 33.359274873015195 38.0 33.0 40.0 8.5 41.0 52-53 33.02289576677075 38.0 33.0 40.0 7.0 41.0 54-55 32.858985722137334 38.0 33.0 40.0 3.5 41.0 56-57 32.49868409926859 37.0 32.5 40.0 2.0 41.0 58-59 32.13933901468252 37.0 32.0 40.0 2.0 41.0 60-61 31.630750554142935 36.0 31.0 39.0 2.0 40.5 62-63 31.36927284971402 36.0 31.0 39.0 2.0 40.0 64-65 30.978910105030472 35.0 30.5 39.0 2.0 40.0 66-67 30.489610952401087 35.0 29.5 38.0 2.0 40.0 68-69 30.111732277995543 35.0 29.0 38.0 2.0 40.0 70-71 29.705775769911398 34.5 29.0 37.0 2.0 39.0 72-73 29.228181565612335 34.0 28.5 36.5 2.0 39.0 74-75 28.785795821732066 34.0 28.0 36.0 2.0 39.0 76-77 27.360155160021982 32.0 26.0 34.5 2.0 36.5 78-79 28.071975164732955 33.5 27.0 35.0 2.0 37.0 80-81 27.767611024877546 34.0 26.0 35.0 2.0 37.0 82-83 27.449375947478725 33.5 26.0 35.0 2.0 36.0 84-85 27.065889406961364 33.0 26.0 35.0 2.0 36.0 86-87 26.78758961412325 33.0 25.0 35.0 2.0 35.5 88-89 26.301491505154885 33.0 24.5 35.0 2.0 35.0 90-91 25.770965326053478 32.0 23.0 35.0 2.0 35.0 92-93 25.212187671754112 32.0 19.5 34.0 2.0 35.0 94-95 24.68629017158802 32.0 17.5 34.0 2.0 35.0 96-97 23.98490904204239 31.0 4.5 34.0 2.0 35.0 98-99 23.2561461548218 31.0 2.0 34.0 2.0 35.0 100-101 20.319376581647752 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25200339564344176 1101 2 0.2510545457616047 1101 3 0.14039202384635274 1101 4 0.20918902529771088 1101 5 0.23594837158542958 1101 6 0.288291392112626 1101 7 0.33734882900785124 1101 8 0.20969038840852505 1101 9 0.30356060247890326 1101 10-11 0.39411938078800546 1101 12-13 0.17873352023438827 1101 14-15 0.44283260119618006 1101 16-17 0.4983658925159844 1101 18-19 0.12884844412972285 1101 20-21 0.19862119434222336 1101 22-23 0.1515167460301612 1101 24-25 0.17513078301499974 1101 26-27 0.2708342380512505 1101 28-29 0.2991468051015076 1101 30-31 0.35070455597620054 1101 32-33 0.09929272149562252 1101 34-35 0.19405332898805483 1101 36-37 0.03777647671880402 1101 38-39 0.04498316657611667 1101 40-41 0.14550684097802957 1101 42-43 0.3201491518848414 1101 44-45 0.3168270836718037 1101 46-47 0.36961697949874406 1101 48-49 0.25121504008195217 1101 50-51 0.48180868589123094 1101 52-53 0.5631412237061681 1101 54-55 0.5056621959069858 1101 56-57 0.36620483712423635 1101 58-59 0.22087713672666354 1101 60-61 0.21558190191097637 1101 62-63 0.6353672378815638 1101 64-65 0.6922121071661067 1101 66-67 0.41662370113171576 1101 68-69 0.43346848407687943 1101 70-71 0.374264517385015 1101 72-73 0.5933582902177648 1101 74-75 0.371974941822117 1101 76-77 0.10656026416247855 1101 78-79 0.09741561377790475 1101 80-81 0.4622312536951867 1101 82-83 0.1939826669932181 1101 84-85 -0.34666960258084956 1101 86-87 -0.2234510406465553 1101 88-89 0.1090749197315084 1101 90-91 0.20932604712080405 1101 92-93 0.19136441513225577 1101 94-95 0.2857716000909569 1101 96-97 0.21856520541284397 1101 98-99 -0.03452281158829251 1101 100-101 0.5527801698179324 1102 1 0.23628929231549733 1102 2 0.17437780731577845 1102 3 0.08273463456499286 1102 4 0.13411880655569064 1102 5 0.20207669456327437 1102 6 0.14553810814519608 1102 7 0.13408684926489656 1102 8 0.0764024490094215 1102 9 -0.012033632491444735 1102 10-11 0.18052673460535829 1102 12-13 0.18253703542716693 1102 14-15 0.25345123540618886 1102 16-17 0.4535588876645136 1102 18-19 0.3073246264998559 1102 20-21 0.38266522645843537 1102 22-23 0.3041556230977065 1102 24-25 0.3042570207089881 1102 26-27 0.31395847557501355 1102 28-29 0.45657155046990994 1102 30-31 0.39859161019358424 1102 32-33 0.35840519915328883 1102 34-35 0.34475409784781874 1102 36-37 0.48875457414553836 1102 38-39 0.38361482317100126 1102 40-41 0.5058353795770572 1102 42-43 0.5056738064653672 1102 44-45 0.391182702589731 1102 46-47 0.2680586908500615 1102 48-49 0.3347345594736737 1102 50-51 0.36770677650887507 1102 52-53 0.40688679070478884 1102 54-55 0.4042900933166962 1102 56-57 0.40801690623650444 1102 58-59 0.5169821243851871 1102 60-61 0.6769864186074308 1102 62-63 0.6614031416464208 1102 64-65 0.7038655812206365 1102 66-67 0.5343110220384624 1102 68-69 0.6897509336470584 1102 70-71 0.7721228457533869 1102 72-73 0.6404491773854666 1102 74-75 0.49033607894017095 1102 76-77 0.40846184443881484 1102 78-79 0.5221024665597049 1102 80-81 0.6591292166423877 1102 82-83 0.6696330769571865 1102 84-85 0.6256666955438099 1102 86-87 0.9202650912228236 1102 88-89 0.8141526568438842 1102 90-91 0.7820077647137929 1102 92-93 0.7309812619560994 1102 94-95 0.7829096543934462 1102 96-97 0.9437262120233356 1102 98-99 0.7557518926418503 1102 100-101 0.9866309230735268 1103 1 0.08317929484223896 1103 2 0.09666998284007633 1103 3 0.17724177962801946 1103 4 0.1503320635252834 1103 5 0.14676562462868503 1103 6 0.16423950346791827 1103 7 0.13628721985362802 1103 8 0.07796060617273781 1103 9 0.14395755198874838 1103 10-11 0.13603503073944267 1103 12-13 0.1881853551441779 1103 14-15 0.2824631391654151 1103 16-17 0.043842514380592945 1103 18-19 0.11101086137450267 1103 20-21 0.09171797920275537 1103 22-23 0.10605734689681157 1103 24-25 0.10803274433963139 1103 26-27 0.18563510883254253 1103 28-29 0.1552646636959949 1103 30-31 0.4324815284956145 1103 32-33 0.30870735530590565 1103 34-35 0.35817390188498877 1103 36-37 0.5351448433838684 1103 38-39 0.5240454921959596 1103 40-41 0.5139630642397393 1103 42-43 0.4827524021586811 1103 44-45 0.6330743194234429 1103 46-47 0.5989600356379583 1103 48-49 0.48805687340724546 1103 50-51 0.7322874778199733 1103 52-53 0.7626028411273111 1103 54-55 0.5345102707500828 1103 56-57 0.4665530807501028 1103 58-59 0.623952772073217 1103 60-61 0.4362757024166406 1103 62-63 0.7529571577614185 1103 64-65 0.7075644498020104 1103 66-67 0.5156096267157402 1103 68-69 0.7534616638753064 1103 70-71 0.5125475488612778 1103 72-73 0.5322537620172625 1103 74-75 0.6867375781400362 1103 76-77 0.7472803121105649 1103 78-79 0.4667054172320988 1103 80-81 0.5109130258453405 1103 82-83 0.6906190675801689 1103 84-85 0.642015062988154 1103 86-87 0.7918978574431002 1103 88-89 0.5709713491148669 1103 90-91 0.5412356346708407 1103 92-93 0.5478732297261111 1103 94-95 0.5717688306757687 1103 96-97 0.7306727854813211 1103 98-99 0.6577827609986286 1103 100-101 0.911202429853617 1104 1 0.16835393527833276 1104 2 0.05392268534393452 1104 3 0.0734088267041173 1104 4 0.16980138381861565 1104 5 0.06350900054277986 1104 6 0.15508059042369382 1104 7 0.1595857288996143 1104 8 0.1389949319925563 1104 9 0.15115567400948748 1104 10-11 0.1539550523663209 1104 12-13 0.0611385870273935 1104 14-15 0.15093957374240574 1104 16-17 0.33464955269643326 1104 18-19 0.18113908273514312 1104 20-21 0.2787425387091673 1104 22-23 0.2639660417901979 1104 24-25 0.29333434000604797 1104 26-27 0.15995405048446543 1104 28-29 0.17429939330872912 1104 30-31 0.2475272631762877 1104 32-33 0.25297195601289246 1104 34-35 0.19259228609737278 1104 36-37 0.08723926248669756 1104 38-39 -0.1933345419553376 1104 40-41 0.14045889456458838 1104 42-43 0.3419005359852392 1104 44-45 0.17531773126826522 1104 46-47 0.1012493361522786 1104 48-49 0.2629457448060464 1104 50-51 0.1723122529107357 1104 52-53 0.21983158981412743 1104 54-55 -0.09763267194211522 1104 56-57 -0.23686957750763327 1104 58-59 -0.2787493895876736 1104 60-61 0.10479508276560878 1104 62-63 -0.013327160358961976 1104 64-65 -0.11417723223502207 1104 66-67 -0.32188938946677226 1104 68-69 0.023585436669929294 1104 70-71 -0.07902447171162663 1104 72-73 -0.05794091407580382 1104 74-75 -0.24277917071103516 1104 76-77 -0.1470508459880513 1104 78-79 -0.11975674086993848 1104 80-81 0.24064724113540592 1104 82-83 0.025342007466353067 1104 84-85 0.44881897361988266 1104 86-87 0.6118678125125818 1104 88-89 0.48719800697564963 1104 90-91 0.559459356478051 1104 92-93 0.6073094937519272 1104 94-95 0.6121754980496483 1104 96-97 1.0434950926445374 1104 98-99 1.2275234438192903 1104 100-101 2.0278386979858034 1105 1 -0.018099061266404703 1105 2 0.11029108758390294 1105 3 -0.026698405513208456 1105 4 0.10772584467885338 1105 5 0.17624580502270248 1105 6 0.016167210225908946 1105 7 -0.02955998776903357 1105 8 0.09169360946187766 1105 9 0.12319189466956715 1105 10-11 0.21127662987138507 1105 12-13 0.06881164791145977 1105 14-15 0.053203364997024494 1105 16-17 -0.04903327823410564 1105 18-19 0.09346483038199693 1105 20-21 -0.04629022669056582 1105 22-23 0.17426629199869836 1105 24-25 0.08572085257604556 1105 26-27 0.07324488808723117 1105 28-29 0.06821098630289413 1105 30-31 0.048706817566859684 1105 32-33 0.09619845428525764 1105 34-35 0.25031896309010193 1105 36-37 0.28904433814498276 1105 38-39 0.282895649871179 1105 40-41 0.03298242607894508 1105 42-43 0.19655322274801534 1105 44-45 0.15042198481287983 1105 46-47 0.18229902041297663 1105 48-49 0.22119071084917863 1105 50-51 0.2591465195615186 1105 52-53 0.3072079748969401 1105 54-55 -0.11232944306090786 1105 56-57 -0.12139239414054259 1105 58-59 -0.13381909052893448 1105 60-61 0.16860420941877052 1105 62-63 0.2971994689750055 1105 64-65 0.49499112234617826 1105 66-67 0.05848711476294355 1105 68-69 -0.2218274073667672 1105 70-71 -0.057375478503001176 1105 72-73 0.16678802670818627 1105 74-75 0.2527051614705442 1105 76-77 0.07368667337332013 1105 78-79 0.03483637243876814 1105 80-81 0.1686408072023653 1105 82-83 0.2925349063106317 1105 84-85 0.32410790487211827 1105 86-87 0.38581634104826534 1105 88-89 0.46195673448667307 1105 90-91 0.43707165243896995 1105 92-93 0.47562237353269765 1105 94-95 0.4943570779376465 1105 96-97 0.5619569060604839 1105 98-99 0.5212682311420629 1105 100-101 0.922685594208847 1106 1 -0.037572691084861276 1106 2 -0.07105515238302829 1106 3 -0.07726508021781342 1106 4 0.08457658015028358 1106 5 0.1439815175860062 1106 6 -0.037289677713737035 1106 7 -0.2747113880928467 1106 8 -0.31424527923563517 1106 9 -0.1770389090095179 1106 10-11 -0.19129665890880432 1106 12-13 -0.08933021023039345 1106 14-15 -0.11463224766617941 1106 16-17 -0.08740008384229014 1106 18-19 -0.09518100653971118 1106 20-21 -0.1426335385511166 1106 22-23 -0.22890639738088225 1106 24-25 -0.11737777293637208 1106 26-27 -0.19019856501139287 1106 28-29 -0.21728971988746792 1106 30-31 -0.3446808292690662 1106 32-33 -0.3505823511162376 1106 34-35 -0.3863288718335909 1106 36-37 -0.6094278792927597 1106 38-39 -0.26168907574802347 1106 40-41 -0.27374877375570605 1106 42-43 -0.21669043015080547 1106 44-45 -0.735059774806814 1106 46-47 -0.8042403023332483 1106 48-49 -1.01133193891485 1106 50-51 -0.590715687197239 1106 52-53 -0.7924871575568204 1106 54-55 -0.9426784044443508 1106 56-57 -1.101545000155216 1106 58-59 -1.1818124764533522 1106 60-61 -1.147515877390969 1106 62-63 -0.911763981367752 1106 64-65 -0.7811034727139372 1106 66-67 -0.4711901045694482 1106 68-69 -0.6272840197199336 1106 70-71 -0.6883513991340919 1106 72-73 -0.6265978977122195 1106 74-75 -0.607539766015762 1106 76-77 -0.8435667628456329 1106 78-79 -1.0749159442100975 1106 80-81 -0.914619036402307 1106 82-83 -0.9220143375489585 1106 84-85 -0.558428524216037 1106 86-87 -0.26108847827034865 1106 88-89 -0.5559202161488983 1106 90-91 -0.715901236740816 1106 92-93 -0.5643290543497663 1106 94-95 -0.6612269931636661 1106 96-97 -0.3684867881763161 1106 98-99 -0.38684779076706377 1106 100-101 0.04843570616585424 1107 1 -0.018786609012774846 1107 2 -0.036496112144476456 1107 3 -0.20934843522206137 1107 4 0.0875238244768326 1107 5 -0.03777677370575816 1107 6 -0.1455592985594194 1107 7 -0.23113031286877117 1107 8 -0.09608331949152671 1107 9 -0.2410131813002181 1107 10-11 -0.04253883570878969 1107 12-13 0.06250488278116251 1107 14-15 0.13851870325353843 1107 16-17 -0.016120112968003752 1107 18-19 -0.08833213082673552 1107 20-21 -0.08858714545807089 1107 22-23 -0.27760960987719585 1107 24-25 -0.1595554397590675 1107 26-27 -0.20307715588187136 1107 28-29 -0.27413686192731035 1107 30-31 -0.23663398542100111 1107 32-33 -0.11004040489477518 1107 34-35 -0.22628743704570553 1107 36-37 -0.09328738242204082 1107 38-39 -0.2902730115327756 1107 40-41 -0.5548751411903865 1107 42-43 -0.4553608762249084 1107 44-45 -0.4572386552006975 1107 46-47 -0.5028932383086939 1107 48-49 -0.11815654320395197 1107 50-51 -0.13362149673591261 1107 52-53 0.1505616800153433 1107 54-55 -0.12249411499028184 1107 56-57 -0.2995521428885226 1107 58-59 -0.3587320860301624 1107 60-61 -0.3989834316982801 1107 62-63 -0.20572473100801503 1107 64-65 -0.16672265407348164 1107 66-67 -0.0674020287732624 1107 68-69 -0.11010938682521854 1107 70-71 -0.13031495732483123 1107 72-73 -0.10339701700626946 1107 74-75 0.019320898674514808 1107 76-77 -0.11737096180196005 1107 78-79 0.10920187621604072 1107 80-81 0.14589655798752688 1107 82-83 0.07379710816106666 1107 84-85 0.25469104721996416 1107 86-87 0.2697353632025816 1107 88-89 0.04203058220757683 1107 90-91 0.005682126931798592 1107 92-93 0.1351716477878533 1107 94-95 0.191738454730789 1107 96-97 0.2584215525251281 1107 98-99 0.05765733223704572 1107 100-101 0.7107638558165199 1108 1 0.0982068550395141 1108 2 0.1270659793587896 1108 3 -0.006243860058660289 1108 4 -0.02636506441205455 1108 5 -0.02552187174497078 1108 6 0.024211943270643133 1108 7 0.11486641915737295 1108 8 0.09934151710978512 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-1.1022859004273755 2316 64-65 -1.316535525280056 2316 66-67 -1.3352457084304632 2316 68-69 -1.8657218946222507 2316 70-71 -1.6302078328706244 2316 72-73 -1.502991571287179 2316 74-75 -2.0040254523140106 2316 76-77 -1.7125449373396187 2316 78-79 -2.0627952490517742 2316 80-81 -1.635306490313397 2316 82-83 -1.9561421427813563 2316 84-85 -1.4928722104482475 2316 86-87 -1.3876114759480167 2316 88-89 -1.8899643734510327 2316 90-91 -1.6897915258764904 2316 92-93 -1.9553335999544004 2316 94-95 -2.403126351495988 2316 96-97 -2.045889189752419 2316 98-99 -2.928836850498289 2316 100-101 -3.608060215997547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 9.0 5 29.0 6 56.0 7 119.0 8 321.0 9 667.0 10 1145.0 11 2158.0 12 2996.0 13 4560.0 14 5740.0 15 8321.0 16 11884.0 17 16162.0 18 13175.0 19 6373.0 20 5052.0 21 4995.0 22 5124.0 23 5562.0 24 6286.0 25 7190.0 26 8468.0 27 10437.0 28 12477.0 29 15657.0 30 18892.0 31 23454.0 32 29495.0 33 37323.0 34 50141.0 35 68021.0 36 93114.0 37 114513.0 38 68226.0 39 4139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.03683623122962 19.82278859528171 15.764928331624247 17.37544684186442 2 15.729839927514346 23.999244941105406 38.01147689519782 22.259438236182422 3 22.121988197418414 28.503174133380043 30.653037926514738 18.72179974268681 4 11.741035567822868 17.873299068073514 39.5612758272886 30.824389536815023 5 10.872739407281912 41.41582524374463 32.784313370206455 14.927121978767 6 27.01197673505626 39.3532079893218 16.62323112139203 17.011584154229908 7 25.335505613905816 37.67205609678023 20.18997892142948 16.802459367884474 8 27.077054556655455 30.409612794511116 19.163984031019925 23.349348617813508 9 27.41361711893991 18.060227938467484 21.557972108642215 32.96818283395039 10-11 25.136648326095752 27.651204015195894 24.900571356094968 22.311576302613382 12-13 29.649860028568064 26.158501414813717 25.761009727033056 18.430628829585157 14-15 24.34399217856428 26.461064575917376 29.694805464435493 19.500137781082845 16-17 19.294290666843832 30.03960536567394 29.31748313412373 21.3486208333585 18-19 18.89300269225949 28.11402919357542 34.11740546515087 18.87556264901422 20-21 22.0579253446598 24.246942585765762 33.66821006411907 20.026922005455365 22-23 20.50774290183667 25.134911910902268 34.19492241998901 20.162422767272044 24-25 21.622974433928647 25.31934940297516 34.2932941155154 18.764382047580796 26-27 20.684221930464968 24.078076858322998 36.5913927919181 18.646308419293927 28-29 18.982905479578232 27.317862044909923 35.02904452789618 18.67018794761566 30-31 19.798768177981522 25.270168858367647 34.39226540192761 20.53879756172322 32-33 19.72472769500028 25.917712773909457 34.69942632097938 19.658133210110883 34-35 21.477952639983087 24.84034019486084 34.441973975772626 19.239733189383443 36-37 20.946506562581398 24.286647213592488 35.52461015208881 19.242236071737302 38-39 19.938620663621606 25.93205239515307 35.15624174247858 18.973085198746745 40-41 19.905085892284717 26.503171604887942 34.19723894468075 19.394503558146592 42-43 19.361675140317313 27.05749650056097 32.302804534246185 21.27802382487554 44-45 20.80058948366576 25.084689203645922 32.429000361632525 21.685720951055792 46-47 22.359684334578773 27.080925313706288 31.43963272526041 19.119757626454533 48-49 21.850996782332572 26.996471286357306 30.720721264840755 20.431810666469367 50-51 19.85608871132129 27.87124235409938 32.101156152279316 20.171512782300013 52-53 21.340407846488702 29.25431480627254 28.66264975648646 20.7426275907523 54-55 20.13867393053922 27.591567223788378 29.71893765863926 22.550821187033144 56-57 22.142435918636092 27.27430606680454 28.83821829381646 21.745039720742902 58-59 23.664817953063217 26.6029736497896 28.47834558257134 21.25386281457584 60-61 21.551589384219806 27.772143582618153 31.149363226870957 19.526903806291084 62-63 21.103158428857387 27.89307447138658 30.480284454163176 20.523482645592857 64-65 20.596523764145143 29.707409012720625 29.503000061919227 20.193067161215005 66-67 21.743118711939445 27.728771024375366 28.303312712244455 22.224797551440734 68-69 21.226126996038687 28.03487344108727 29.09220488901606 21.64679467385798 70-71 22.975961200905655 28.109487855276477 28.455185732394057 20.45936521142381 72-73 22.177419354838708 26.942746616255263 30.303540475083196 20.57629355382283 74-75 20.574872144098546 30.68541634894582 28.397071280703155 20.342640226252477 76-77 21.138242686290187 28.251164750142127 28.685129022832957 21.925463540734736 78-79 21.143852066953734 28.396367664147103 28.940221392184 21.519558876715163 80-81 22.24481674649422 28.509045822248968 28.066552242558974 21.17958518869784 82-83 21.671544685979395 27.7817546975477 29.393464360254562 21.153236256218342 84-85 20.70424130743437 28.67821647584811 29.050463704195995 21.567078512521523 86-87 20.715667997822433 29.38743428220653 27.779401138466625 22.11749658150441 88-89 20.189284217125465 29.245242402383802 26.96272853274519 23.602744847745548 90-91 21.022337574440954 27.30370399465808 28.05967060160076 23.614287829300203 92-93 21.95570659869725 28.608552817898612 28.21138487680544 21.224355706598697 94-95 21.054491846463378 27.772021194965895 28.596890841092133 22.576596117478598 96-97 19.208539781126937 28.220075677170165 30.33432267119712 22.23706187050577 98-99 20.42160369554789 29.250956559182022 27.390718070942132 22.936721674327952 100-101 19.50346840809814 28.44714133970757 28.07756058570844 23.971829666485856 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 811.0 1 586.5 2 444.5 3 539.5 4 484.0 5 438.0 6 674.0 7 1242.0 8 1954.0 9 2363.0 10 2210.5 11 1873.0 12 1802.5 13 1943.5 14 2099.0 15 2244.0 16 2339.5 17 2451.0 18 2526.0 19 2636.5 20 2865.5 21 3084.0 22 3340.5 23 3711.5 24 4042.5 25 4479.5 26 5303.5 27 6105.5 28 6811.5 29 7990.5 30 9452.5 31 10751.0 32 12508.5 33 14380.0 34 15532.5 35 16598.0 36 18842.5 37 20776.0 38 20555.0 39 19411.5 40 18215.0 41 17189.5 42 16344.0 43 16594.0 44 17396.5 45 17721.5 46 18511.0 47 28331.0 48 49272.0 49 46603.0 50 25659.5 51 16765.5 52 13860.5 53 13168.0 54 12543.0 55 11884.0 56 11422.0 57 10788.5 58 10700.0 59 10237.0 60 8851.0 61 7726.5 62 6550.5 63 4718.0 64 3158.0 65 2361.5 66 1797.5 67 1378.5 68 976.5 69 631.5 70 513.0 71 432.0 72 369.0 73 390.5 74 348.5 75 318.5 76 256.5 77 148.0 78 103.0 79 80.5 80 50.0 81 38.5 82 36.0 83 24.5 84 12.5 85 6.5 86 2.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020685989696263233 2 0.012683380543694245 3 0.008455587029129496 4 0.0 5 0.0019629041317622046 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.059557531210175E-4 14-15 2.2648893828025439E-4 16-17 0.0 18-19 0.002264889382802544 20-21 7.54963127600848E-5 22-23 0.0 24-25 0.0 26-27 0.0020384004445222897 28-29 0.0037748156380042397 30-31 0.0019629041317622046 32-33 0.010116505909851362 34-35 0.002491378321082798 36-37 9.814520658811023E-4 38-39 0.0013589336296815264 40-41 1.509926255201696E-4 42-43 0.005284741893205935 44-45 0.0014344299424416112 46-47 7.54963127600848E-5 48-49 4.5297787656050877E-4 50-51 1.509926255201696E-4 52-53 7.54963127600848E-5 54-55 0.0016609188807218656 56-57 0.014872773613736704 58-59 0.03050051035507426 60-61 0.023630345893906543 62-63 0.015627736741337554 64-65 0.019780033943142216 66-67 0.0025668746338428834 68-69 5.284741893205935E-4 70-71 7.54963127600848E-5 72-73 0.0 74-75 0.002264889382802544 76-77 0.0026423709466029677 78-79 0.009890016971571108 80-81 0.019251559753821625 82-83 0.01962904131762205 84-85 0.033822348116517985 86-87 0.011701928477813144 88-89 0.047789165977133674 90-91 0.05284741893205936 92-93 0.03344486655271756 94-95 0.036238230124840703 96-97 0.059415598142186735 98-99 0.061378502273948934 100-101 0.06839965936063683 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 662284.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.04110751456191 #Duplication Level Percentage of deduplicated Percentage of total 1 77.19189218029358 33.9961642276478 2 12.208369028621302 10.75340185933917 3 4.248866793616874 5.613743978181981 4 1.9321961282411926 3.4038422969236244 5 1.0542403920666679 2.321495722660101 6 0.6836111409293754 1.8064195053493775 7 0.42289438517344746 1.3037315959309848 8 0.30911561467951615 1.089103521642437 9 0.23828723584503608 0.9444990395879104 >10 1.4652227895313024 12.512434204408867 >50 0.15322930443476854 4.730983301848111 >100 0.0776453413587042 6.422316940833089 >500 0.004809888402751583 1.369499131381323 >1k 0.007901959518806172 7.546090009971201 >5k 0.0013742538293575952 4.086860185461223 >10k+ 3.435634573393988E-4 2.09941447883282 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13875 2.095022679092353 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8489 1.2817763980407197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7117 1.074614515827047 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5805 0.8765121911445845 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5599 0.8454077102874294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4316 0.651684171745052 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4009 0.6053294357103599 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3681 0.5558038545397442 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3640 0.5496131568934174 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3588 0.5417615403663685 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3554 0.5366277910986827 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2910 0.4393885402636935 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2815 0.4250442408392774 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2315 0.3495479280791926 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2188 0.33037186463813106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1847 0.27888337933575325 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1577 0.23811537044530745 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1472 0.22226114476568964 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1468 0.22165717426360898 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1341 0.2024811108225474 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1293 0.19523346479757928 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1249 0.1885897892746918 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1166 0.17605740135651773 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1131 0.1707726594633118 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1101 0.16624288069770674 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1096 0.16548791757010586 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1085 0.163826998689384 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1030 0.15552240428577468 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 970 0.1464628467545645 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 751 0.11339546176564737 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 742 0.11203652813596583 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 728 0.10992263137868347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.509926255201696E-4 0.0 20-21 0.0 0.0 0.0 1.509926255201696E-4 0.0 22-23 0.0 0.0 0.0 6.039705020806784E-4 0.0 24-25 0.0 0.0 0.0 9.814520658811025E-4 0.0 26-27 0.0 0.0 0.0 0.0015099262552016961 0.0 28-29 0.0 0.0 0.0 0.003321837761443731 0.0 30-31 0.0 0.0 0.0 0.010493987473651788 0.0 32-33 0.0 0.0 0.0 0.017892626124140096 0.0 34-35 0.0 0.0 0.0 0.026121724214989338 0.0 36-37 0.0 0.0 0.0 0.030349517729554087 0.0 38-39 0.0 0.0 0.0 0.036313726437600787 0.0 40-41 0.0 0.0 0.0 0.0446938171539702 0.0 42-43 0.0 0.0 0.0 0.05360238205966021 0.0 44-45 0.0 0.0 0.0 0.06273743590363047 0.0 46-47 0.0 0.0 0.0 0.07338241600280243 0.0 48-49 0.0 0.0 0.0 0.08666976704857735 0.0 50-51 0.0 0.0 0.0 0.1047888821109977 0.0 52-53 0.0 0.0 0.0 0.14661383938008468 0.0 54-55 0.0 0.0 0.0 0.19236460491269608 0.0 56-57 0.0 0.0 0.0 0.2573669301991291 0.0 58-59 0.0 0.0 0.0 0.33837447379070007 0.0 60-61 0.0 0.0 0.0 0.4577341442643941 0.0 62-63 0.0 0.0 0.0 0.5349668722179609 0.0 64-65 0.0 0.0 0.0 0.6131055559246485 0.0 66-67 0.0 0.0 0.0 0.7802543923754763 0.0 68-69 0.0 0.0 0.0 0.9895301713464315 0.0 70-71 0.0 0.0 0.0 1.13961684111348 0.0 72-73 0.0 0.0 0.0 1.2656956834228215 0.0 74-75 0.0 0.0 0.0 1.5498638046517808 0.0 76-77 0.0 0.0 0.0 1.726298687572099 0.0 78-79 0.0 0.0 0.0 1.8523020335686806 0.0 80-81 0.0 0.0 0.0 2.005559548471653 0.0 82-83 0.0 0.0 0.0 2.260435100349699 0.0 84-85 0.0 0.0 0.0 2.5582680541882334 0.0 86-87 0.0 0.0 0.0 2.9465455907133498 0.0 88-89 0.0 0.0 0.0 3.535416830242011 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTAC 20 0.0020722037 71.262856 1 CCGTACA 25 0.0050212536 56.99737 2 CTTTGCG 110 2.732124E-6 30.22588 2 GATAGTC 85 6.4116745E-4 27.935669 7 GTAAGCG 105 4.1836756E-11 27.158012 94-95 ACCGGTG 90 8.953824E-4 26.383688 7 CATATAG 95 0.0012263351 24.998846 2 TTGCGTT 160 1.8607625E-6 23.745317 4 GAGTACT 7155 0.0 23.662352 12-13 GTATAGG 305 0.0 23.364872 1 GTACATG 7165 0.0 23.008339 1 TCTATAC 105 0.0021967979 22.616297 3 ATGGGCG 105 0.002197762 22.614588 5 GTACTTT 7565 0.0 22.25437 14-15 TTTGCGT 150 3.0335563E-5 22.16397 3 TACATGG 7415 0.0 21.97134 2 AGTACTT 7345 0.0 21.8541 12-13 ACATGGG 7225 0.0 21.429918 3 ATGGGAG 2035 0.0 20.769861 5 TGGGATA 460 0.0 20.648102 6 >>END_MODULE