##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936558_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 250811 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61107367699184 31.0 31.0 34.0 30.0 34.0 2 31.828416616496085 31.0 31.0 34.0 30.0 34.0 3 32.06966999055065 33.0 31.0 34.0 30.0 34.0 4 35.854639549302064 37.0 35.0 37.0 35.0 37.0 5 35.72648328821304 37.0 35.0 37.0 33.0 37.0 6 35.86548835577387 37.0 35.0 37.0 35.0 37.0 7 35.82212901348027 37.0 35.0 37.0 35.0 37.0 8 35.80789917507605 37.0 35.0 37.0 35.0 37.0 9 37.453760002551725 39.0 37.0 39.0 35.0 39.0 10-11 37.33059754157513 39.0 37.0 39.0 34.0 39.0 12-13 37.226262006052366 39.0 37.0 39.0 33.5 39.0 14-15 38.21700403889781 40.0 37.5 41.0 33.0 41.0 16-17 38.21251061556311 40.0 37.5 41.0 33.5 41.0 18-19 38.37610790595309 40.0 38.0 41.0 33.5 41.0 20-21 38.47546957669321 40.0 38.0 41.0 34.0 41.0 22-23 38.388906387678375 40.0 38.0 41.0 34.0 41.0 24-25 38.24564512720734 40.0 37.5 41.0 33.5 41.0 26-27 37.962220556514666 40.0 37.0 41.0 33.0 41.0 28-29 37.86965882676597 40.0 37.0 41.0 33.0 41.0 30-31 37.791015944276765 40.0 37.0 41.0 33.0 41.0 32-33 37.501816108543885 39.0 36.0 41.0 33.0 41.0 34-35 37.24541786444773 39.0 35.0 41.0 33.0 41.0 36-37 36.93103372659094 39.0 35.0 41.0 31.5 41.0 38-39 36.69126752813872 39.0 35.0 41.0 31.0 41.0 40-41 36.4051676361882 39.0 35.0 41.0 30.5 41.0 42-43 36.06422963905092 39.0 35.0 40.0 30.0 41.0 44-45 35.56287403662519 39.0 35.0 40.0 28.5 41.0 46-47 34.874519060168815 38.0 35.0 40.0 25.0 41.0 48-49 34.08791879144057 38.0 34.0 40.0 20.5 41.0 50-51 33.83682932566754 38.0 34.0 40.0 19.5 41.0 52-53 33.592342441121005 38.0 33.0 40.0 16.5 41.0 54-55 33.34244909513538 38.0 33.0 40.0 13.5 41.0 56-57 33.000448544920275 37.5 33.0 40.0 8.5 41.0 58-59 32.58463743615711 37.0 32.5 40.0 7.5 41.0 60-61 32.11384269429969 36.0 31.0 39.0 3.5 41.0 62-63 31.86891723249778 36.0 31.0 39.0 2.0 40.0 64-65 31.434994876620244 35.5 31.0 39.0 2.0 40.0 66-67 30.99315620128304 35.0 30.5 38.5 2.0 40.0 68-69 30.466688063920643 35.0 30.0 38.0 2.0 40.0 70-71 29.9205397689894 34.5 29.0 37.0 2.0 39.0 72-73 29.4827379979347 34.0 29.0 36.5 2.0 39.0 74-75 29.1220879467009 34.0 29.0 36.0 2.0 39.0 76-77 27.75745880364099 32.5 26.5 34.5 2.0 36.5 78-79 28.500009967664894 34.0 28.5 35.0 2.0 37.0 80-81 28.265422967892157 34.0 28.0 35.0 2.0 37.0 82-83 27.910560142896443 34.0 27.5 35.0 2.0 36.5 84-85 27.472329762251256 33.0 26.0 35.0 2.0 36.0 86-87 27.152628871939427 33.0 26.0 35.0 2.0 36.0 88-89 26.738855153880813 33.0 25.0 35.0 2.0 35.0 90-91 26.23654464915813 33.0 24.5 35.0 2.0 35.0 92-93 25.79460031657304 32.5 23.5 35.0 2.0 35.0 94-95 25.234449445997186 32.0 19.5 34.0 2.0 35.0 96-97 24.534557894191245 32.0 12.5 34.0 2.0 35.0 98-99 23.794935230113513 31.0 2.0 34.0 2.0 35.0 100-101 21.271886001810127 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43170316922497776 1101 2 0.37420793607809344 1101 3 0.20159539966541118 1101 4 0.18410172512154332 1101 5 0.332089555060854 1101 6 0.08485921516052741 1101 7 0.10482824324409279 1101 8 0.09701604603177572 1101 9 0.2454673868268742 1101 10-11 0.2778132784801173 1101 12-13 0.22791949340177808 1101 14-15 0.22664042218169556 1101 16-17 0.28655946907505125 1101 18-19 0.3413350372337689 1101 20-21 0.06796774792894666 1101 22-23 0.04040209140519124 1101 24-25 -0.2492680933171627 1101 26-27 0.0533059213083078 1101 28-29 0.09961659859570915 1101 30-31 0.12226018905702318 1101 32-33 0.1690998763792919 1101 34-35 0.29411405306231586 1101 36-37 0.21321995622768952 1101 38-39 0.416864881347891 1101 40-41 0.7621855640993971 1101 42-43 0.7557927747630586 1101 44-45 0.568053731569087 1101 46-47 0.7413622995956501 1101 48-49 0.9301733750844008 1101 50-51 1.0866624723406488 1101 52-53 1.3615314199087791 1101 54-55 1.3796609160975493 1101 56-57 1.458483694015058 1101 58-59 1.2346234507112115 1101 60-61 1.5482718262074826 1101 62-63 1.4388660839091365 1101 64-65 1.2408312798848051 1101 66-67 1.1962803369463195 1101 68-69 1.0669699417699725 1101 70-71 1.0590995277454347 1101 72-73 1.1904052860693888 1101 74-75 1.2349145598926263 1101 76-77 1.6811628827341139 1101 78-79 1.3668286461897878 1101 80-81 1.4295633051948116 1101 82-83 1.6879775863388247 1101 84-85 1.8528479252751922 1101 86-87 1.4054902128267912 1101 88-89 1.4194795237839024 1101 90-91 1.5053375315082889 1101 92-93 1.5051759245219607 1101 94-95 1.168465820162968 1101 96-97 1.663831795387182 1101 98-99 1.3908764644192964 1101 100-101 0.5330167809838748 1102 1 0.40245646707269955 1102 2 0.30778330443418156 1102 3 0.3248114761909058 1102 4 0.17997941547099572 1102 5 0.17955445516113855 1102 6 -0.13632318860403814 1102 7 -0.09712153689939385 1102 8 0.12194276052687769 1102 9 -0.07850275897271075 1102 10-11 0.12065685249847746 1102 12-13 0.22101040599917354 1102 14-15 0.2766794756415436 1102 16-17 0.49423950070353584 1102 18-19 0.47206528871876685 1102 20-21 0.22381757050088424 1102 22-23 -0.04252865379267945 1102 24-25 0.07572660312656154 1102 26-27 0.13782050126665268 1102 28-29 -0.01190513634218604 1102 30-31 0.1894875862644625 1102 32-33 0.22720033537797946 1102 34-35 0.5765621746391147 1102 36-37 0.29771042911204404 1102 38-39 0.32857513360431057 1102 40-41 0.6487473167801028 1102 42-43 0.5634376100635237 1102 44-45 0.607256655275016 1102 46-47 0.7301838493912172 1102 48-49 0.5902009535770318 1102 50-51 0.9087088736366411 1102 52-53 0.6652709092245246 1102 54-55 0.6331323347506412 1102 56-57 1.2304235420440648 1102 58-59 1.3415551987633592 1102 60-61 1.2432618940930098 1102 62-63 0.9712876877045566 1102 64-65 1.07051239151021 1102 66-67 1.151405018732941 1102 68-69 1.1296073520916607 1102 70-71 0.773939360345917 1102 72-73 0.9898440733871361 1102 74-75 0.32626831673903567 1102 76-77 0.7142860023823445 1102 78-79 1.1606962887108097 1102 80-81 1.081625906444522 1102 82-83 1.1499028736951473 1102 84-85 1.1211355515962609 1102 86-87 0.9289198780277488 1102 88-89 1.0729138175430641 1102 90-91 1.2780946093452101 1102 92-93 1.2859727115311408 1102 94-95 1.0351392368103483 1102 96-97 0.6104466800589385 1102 98-99 0.018400475064975552 1102 100-101 0.008818100122962136 1103 1 0.42837992626630594 1103 2 0.40382647604474897 1103 3 0.15542096779737946 1103 4 0.14206763720366666 1103 5 -0.1787630815979142 1103 6 -0.16146108842814044 1103 7 -0.10251798822032754 1103 8 0.13534850194123038 1103 9 0.0890775473954406 1103 10-11 0.014802727457912113 1103 12-13 8.915474510828858E-4 1103 14-15 0.021594686461767765 1103 16-17 -0.025049397293294362 1103 18-19 -0.07424855932672614 1103 20-21 -0.5358781106303212 1103 22-23 -0.18515891149030494 1103 24-25 -0.5651105299232952 1103 26-27 -0.4535434688820885 1103 28-29 -0.028188415881778894 1103 30-31 0.0018584385963791306 1103 32-33 -0.1577322943673991 1103 34-35 -0.09444214593742117 1103 36-37 0.18125979127199798 1103 38-39 -0.05799692439045856 1103 40-41 0.35817573695189964 1103 42-43 0.21301836044349187 1103 44-45 0.6406002350349098 1103 46-47 0.5890990943661976 1103 48-49 0.6505438673191932 1103 50-51 0.5860318239243938 1103 52-53 1.1782924096429142 1103 54-55 1.1158414899327695 1103 56-57 0.7116725814415759 1103 58-59 0.5950774078929157 1103 60-61 0.5180966704647432 1103 62-63 0.26912569170065836 1103 64-65 0.6757346535796422 1103 66-67 0.668858098637994 1103 68-69 1.1054257854130398 1103 70-71 0.9013195198486237 1103 72-73 1.0335109279049597 1103 74-75 0.8158596550931172 1103 76-77 0.8932910743634892 1103 78-79 0.8269360209375307 1103 80-81 0.9624941203215975 1103 82-83 0.9285288278936221 1103 84-85 1.0587518912311467 1103 86-87 1.384698008737999 1103 88-89 1.1473711838698897 1103 90-91 0.8670335918750496 1103 92-93 1.0339362342525895 1103 94-95 1.260535005036182 1103 96-97 1.2595834186213644 1103 98-99 0.7666030810727982 1103 100-101 0.9306378654797918 1104 1 0.1560135140525638 1104 2 0.46128921686626967 1104 3 0.17770377404965032 1104 4 0.0850992620673523 1104 5 0.10857970284411067 1104 6 0.21477300826397538 1104 7 0.16702036147069776 1104 8 0.11083378002192745 1104 9 0.28347231293524544 1104 10-11 0.2064893868544999 1104 12-13 0.05219289241654934 1104 14-15 0.28376480641814794 1104 16-17 0.2103340996169223 1104 18-19 -0.02484107114060663 1104 20-21 0.09112661492402196 1104 22-23 0.16216933278450085 1104 24-25 0.02150161111632798 1104 26-27 0.333170453328016 1104 28-29 0.378452805492266 1104 30-31 0.11289442551022688 1104 32-33 0.10913066455663767 1104 34-35 0.3373899151312827 1104 36-37 0.48489483307497494 1104 38-39 0.5345003493281908 1104 40-41 0.44208704193190584 1104 42-43 0.3103247944674905 1104 44-45 0.5760709729484006 1104 46-47 0.901676339004517 1104 48-49 0.7918851977444987 1104 50-51 1.0651230634736493 1104 52-53 1.1879107302536056 1104 54-55 0.7018284037822795 1104 56-57 0.9897460092859944 1104 58-59 0.9050483275584966 1104 60-61 0.7824405454193055 1104 62-63 0.86241903957416 1104 64-65 1.080812806978411 1104 66-67 0.9290943763551844 1104 68-69 1.1597224048241621 1104 70-71 1.1680588873513464 1104 72-73 0.9457501136555955 1104 74-75 1.0517556928975544 1104 76-77 1.1366062324878072 1104 78-79 0.9588298777168802 1104 80-81 0.7796987731751059 1104 82-83 0.8989504927427703 1104 84-85 1.0055203390682976 1104 86-87 1.028587503467186 1104 88-89 1.1406063710745435 1104 90-91 1.032086299981195 1104 92-93 0.9038889787091513 1104 94-95 0.956187495039817 1104 96-97 0.641411844648264 1104 98-99 0.2211905038281614 1104 100-101 0.3293837760577638 1105 1 0.20290540594445616 1105 2 0.2722158577929079 1105 3 -0.033493137147260654 1105 4 0.07826528523337828 1105 5 -0.14699944773504114 1105 6 -0.11265942416846286 1105 7 -0.07574025628991876 1105 8 -0.17406969488154544 1105 9 -0.40772459826166596 1105 10-11 -0.11771910735399871 1105 12-13 -0.4414654087417418 1105 14-15 0.00888063653397353 1105 16-17 0.34239008417291217 1105 18-19 0.11693499063544976 1105 20-21 -0.10896025766284367 1105 22-23 0.08277744089261319 1105 24-25 0.08306531768003111 1105 26-27 0.02622064637821353 1105 28-29 -0.02972287018341291 1105 30-31 -0.08431599920019295 1105 32-33 -0.06706624664027316 1105 34-35 0.10894396918534 1105 36-37 0.18706664774678927 1105 38-39 0.031787994115831 1105 40-41 -0.24323149088662888 1105 42-43 -0.19096864182594686 1105 44-45 -0.7366992973218771 1105 46-47 -0.21212674979857127 1105 48-49 0.184134232493534 1105 50-51 0.3983470016975872 1105 52-53 0.0877080275508959 1105 54-55 -0.0569071174532354 1105 56-57 0.0695915691315534 1105 58-59 0.07755798006814985 1105 60-61 0.1612436342223944 1105 62-63 0.03360629826141803 1105 64-65 -0.0073339111683132785 1105 66-67 -0.5241006043397967 1105 68-69 -0.5864841588824028 1105 70-71 -0.47605308176062167 1105 72-73 -0.1884390755335943 1105 74-75 -0.29086013721827797 1105 76-77 -0.5317914509098749 1105 78-79 -8.61241974234872E-4 1105 80-81 -0.22409466311832915 1105 82-83 -0.07081784702556959 1105 84-85 -0.19974993120197482 1105 86-87 -0.5558623034826198 1105 88-89 -0.6468357138675422 1105 90-91 -0.8186955533006604 1105 92-93 -0.28591797109779904 1105 94-95 0.07212378847610879 1105 96-97 -0.08736228662586498 1105 98-99 -0.08626123362357774 1105 100-101 0.11007875675274548 1106 1 0.30524428328333286 1106 2 0.4922782278552802 1106 3 0.3128686092144868 1106 4 0.029824536792631307 1106 5 0.039019263283670114 1106 6 -0.1629192994283386 1106 7 -0.07308952863919416 1106 8 -0.0194958903077449 1106 9 -0.2613628518999178 1106 10-11 -0.04136775600264997 1106 12-13 -0.07231260208893531 1106 14-15 0.16475381740715989 1106 16-17 -0.036534032251203996 1106 18-19 0.19224684094729838 1106 20-21 0.08519254898996564 1106 22-23 -0.010907590292418945 1106 24-25 0.21139172100644288 1106 26-27 0.04113748629504954 1106 28-29 -0.12616257912312534 1106 30-31 -0.29655612394032005 1106 32-33 0.11391088433685326 1106 34-35 -0.32684085409948693 1106 36-37 -0.3494458262656863 1106 38-39 -0.0815436067157691 1106 40-41 0.07186726171212854 1106 42-43 -0.3087960846533875 1106 44-45 -0.5212367193592939 1106 46-47 -0.34310388077570764 1106 48-49 -0.45102689016759 1106 50-51 -0.19619561784503503 1106 52-53 -0.09324311590024337 1106 54-55 -0.7044902775364008 1106 56-57 -0.894649595109609 1106 58-59 -0.7766000240898521 1106 60-61 -0.05777923480047065 1106 62-63 -0.16148205932693926 1106 64-65 0.08663698280258458 1106 66-67 0.15316031042111788 1106 68-69 0.11692020702196615 1106 70-71 -0.06435869506623604 1106 72-73 -0.21848065557517415 1106 74-75 -0.6842333180914544 1106 76-77 -0.020097064215487848 1106 78-79 0.30069800958501247 1106 80-81 0.30519327866961277 1106 82-83 0.6274669762592495 1106 84-85 0.8685972621452187 1106 86-87 0.3273237672034526 1106 88-89 0.28687010733828444 1106 90-91 0.45367970657460077 1106 92-93 0.5155373303575033 1106 94-95 0.6582204620727872 1106 96-97 1.1259173391537622 1106 98-99 0.846794899432556 1106 100-101 1.0857024573764456 1107 1 0.8891385140525649 1107 2 0.6681195740091255 1107 3 0.606498416906792 1107 4 0.5071081906387818 1107 5 0.10067791712982199 1107 6 0.33539800826397226 1107 7 0.37907393289926716 1107 8 0.4760569943076405 1107 9 0.3182044557923902 1107 10-11 0.17113224399735572 1107 12-13 0.3976393209879845 1107 14-15 0.5866889135610052 1107 16-17 0.5057135639026384 1107 18-19 0.07692232171653046 1107 20-21 0.2184257220668826 1107 22-23 0.7949818327845009 1107 24-25 0.25261768254490136 1107 26-27 0.006161524756585379 1107 28-29 0.1576715554922643 1107 30-31 -0.24721718163262807 1107 32-33 -0.6106684425862241 1107 34-35 -0.7146413348687162 1107 36-37 -0.9038328454964528 1107 38-39 -1.1180219721003866 1107 40-41 -1.0402120652109517 1107 42-43 -1.5253225269610837 1107 44-45 -1.1148665270516034 1107 46-47 -1.9966495538526274 1107 48-49 -1.7116639093983608 1107 50-51 -1.7842965793834935 1107 52-53 -1.6861517697463952 1107 54-55 -1.7761849890748636 1107 56-57 -1.7229548835711483 1107 58-59 -2.030866851012931 1107 60-61 -2.0737648117235494 1107 62-63 -1.6307729247115539 1107 64-65 -1.7350577287358782 1107 66-67 -1.635481516501958 1107 68-69 -1.767755273747266 1107 70-71 -2.0800437912200813 1107 72-73 -2.217999886344405 1107 74-75 -1.8764809142453025 1107 76-77 -1.4131259103693345 1107 78-79 -1.1110808365688314 1107 80-81 -1.695167298253466 1107 82-83 -1.829263792971517 1107 84-85 -2.0154171609317046 1107 86-87 -1.5329526751042408 1107 88-89 -1.3911347003540264 1107 90-91 -1.907377985733092 1107 92-93 -1.97785209271942 1107 94-95 -2.0622723263887544 1107 96-97 -1.6893470839231632 1107 98-99 -1.2143228890289812 1107 100-101 -0.48557158108509313 1108 1 0.40290093127110893 1108 2 0.2649221402342903 1108 3 0.06691853280083038 1108 4 0.14757797540699613 1108 5 -0.1472008080357412 1108 6 0.0689451936944323 1108 7 0.07288601899198 1108 8 0.2584969777513493 1108 9 0.02381289950100296 1108 10-11 0.07469184664636686 1108 12-13 0.46665836072308053 1108 14-15 0.42415269667358757 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-2.050189037387266 2316 62-63 -1.9509817189593441 2316 64-65 -2.0018926291783536 2316 66-67 -1.6480990496877972 2316 68-69 -2.163185306933105 2316 70-71 -2.0118399748483995 2316 72-73 -2.153010948291307 2316 74-75 -1.6956526509709668 2316 76-77 -2.0498792289534045 2316 78-79 -1.7547478719670622 2316 80-81 -1.9527032495808996 2316 82-83 -2.3353478637679785 2316 84-85 -2.6142141742060403 2316 86-87 -2.60060339413079 2316 88-89 -2.0133000764602222 2316 90-91 -2.709645684848134 2316 92-93 -2.7393979997990634 2316 94-95 -2.5941791294861005 2316 96-97 -2.6617613538346667 2316 98-99 -2.757153364692698 2316 100-101 -2.915812178430226 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 13.0 6 29.0 7 54.0 8 106.0 9 219.0 10 407.0 11 704.0 12 1107.0 13 1470.0 14 1910.0 15 2827.0 16 3921.0 17 5285.0 18 4318.0 19 2398.0 20 1937.0 21 1958.0 22 2034.0 23 2181.0 24 2528.0 25 2733.0 26 3242.0 27 3874.0 28 4819.0 29 5677.0 30 6960.0 31 8665.0 32 10719.0 33 13825.0 34 18640.0 35 25783.0 36 36030.0 37 44938.0 38 27742.0 39 1756.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.49074346639138 19.71071064209011 15.386290410384415 18.41225548113409 2 15.884250527348346 24.019570704553338 37.79004158974093 22.306137178357385 3 21.97740856369342 27.661949817187192 30.257213829180674 20.103427789938717 4 12.234831568202118 18.168623197652163 39.22060418527498 30.375941048870743 5 12.023396103041732 40.32359027315388 33.18355255550993 14.469461068294454 6 28.453696209496393 39.16534761234555 16.45900698135249 15.921949196805564 7 26.534721363895525 36.43101777832711 20.178939520196483 16.855321337580886 8 26.7803246269103 31.619426580173915 19.36318582518311 22.237062967732676 9 27.07597349261158 18.74018134115902 20.692350018740182 33.49149514748922 10-11 25.09309836130936 27.100594075196366 25.94733064869822 21.85897691479606 12-13 29.01912001291812 25.676157896100843 26.030608766379398 19.27411332460164 14-15 23.928575700427814 26.606687904438004 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40-41 20.059131135154317 27.62456713257854 32.638153736964135 19.678147995303004 42-43 20.101162753604697 28.350293479647824 30.606617647058826 20.941926119688656 44-45 21.268388179732185 26.400631562579118 30.79485579520467 21.536124462484025 46-47 22.09210993225921 28.326335976779145 29.902236363128914 19.679317727832732 48-49 22.433249330100804 28.25658734209519 29.046310769427073 20.26385255837693 50-51 20.642119532714005 28.533950001993542 30.688369682229578 20.135560783062875 52-53 21.507987296878646 30.09714737973202 27.67274909341738 20.722116229971952 54-55 20.085053442511168 28.555108290617206 28.825859755526178 22.533978511345442 56-57 22.137360446570973 27.8761961722488 28.540669856459328 21.44577352472089 58-59 23.628737405754975 27.336738314813264 27.686606142522795 21.347918136908962 60-61 21.116460819203127 28.238484005701924 30.598203694289104 20.046851480805845 62-63 21.036850373607447 28.32379046084897 29.926275329149355 20.71308383639423 64-65 20.676894119148358 29.99046908834238 28.850747518573318 20.481889273935945 66-67 21.328165961934495 27.92493755096101 28.28537765470715 22.461518832397346 68-69 20.808497234969757 28.758507401987956 28.49496234216203 21.938033020880265 70-71 22.41371404876629 28.700183606217493 28.1756798513607 20.710422493655518 72-73 21.747650621384228 27.486832714673596 29.60914792413411 21.156368739808062 74-75 20.46002767023775 30.535343346184973 27.81257600344483 21.192052980132452 76-77 21.04586395116564 28.342709732981934 28.164285685806217 22.44714063004621 78-79 21.081289077453107 28.029898239122115 28.571400089319894 22.317412594104887 80-81 21.735186038118822 28.535681315635735 27.986968060183 21.742164586062444 82-83 21.47966935030445 28.225648878095853 28.491420732995987 21.80326103860371 84-85 20.38287165705035 28.792132031309187 28.30606605552875 22.51893025611171 86-87 20.500275139364067 29.0612962652822 27.28664736703591 23.151781228317823 88-89 20.25237448576904 28.922961571517735 26.63930748568728 24.185356457025943 90-91 20.97932357439545 27.14137299123211 27.715862162480132 24.163441271892307 92-93 21.69846114587715 28.143147922576837 27.688010733502523 22.47038019804349 94-95 21.243241303411647 28.12534890026636 27.405418122079205 23.22599167424279 96-97 19.701337799310167 28.163765775465038 28.915206252367565 23.219690172857227 98-99 20.820515069891517 29.67324144723594 25.674342006886476 23.83190147598607 100-101 19.743400590158704 28.80393173299306 26.293763458011004 25.15890421883723 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 434.0 1 296.5 2 197.0 3 245.5 4 234.0 5 213.0 6 286.5 7 540.5 8 832.5 9 947.0 10 835.5 11 710.0 12 697.0 13 718.5 14 756.0 15 761.0 16 764.0 17 801.5 18 859.0 19 903.0 20 918.5 21 979.5 22 1058.5 23 1131.0 24 1272.5 25 1461.0 26 1658.0 27 1942.5 28 2297.5 29 2608.5 30 3102.5 31 3713.5 32 4305.5 33 4819.5 34 5195.0 35 5672.5 36 6523.5 37 7013.5 38 7166.5 39 7174.5 40 6984.5 41 7147.0 42 7301.0 43 7482.5 44 7497.5 45 7552.0 46 7747.0 47 11098.5 48 18312.0 49 16660.0 50 9540.5 51 7162.0 52 6240.5 53 6012.0 54 5666.5 55 5183.0 56 4754.0 57 4272.5 58 4017.0 59 3688.5 60 3092.5 61 2662.5 62 2193.0 63 1627.0 64 1177.5 65 865.5 66 671.5 67 564.5 68 402.5 69 252.0 70 202.5 71 162.0 72 148.5 73 137.0 74 99.5 75 87.5 76 80.0 77 63.0 78 42.0 79 33.5 80 29.5 81 19.5 82 15.0 83 13.0 84 10.5 85 7.0 86 3.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1259474265482774 2 0.011163784682490003 3 0.0031896527664257153 4 0.010765078086686789 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005183185745441786 10-11 3.987065958032144E-4 12-13 1.993532979016072E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.96766489508036E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004983832447540179 34-35 9.96766489508036E-4 36-37 0.002192886276917679 38-39 0.0 40-41 0.004983832447540179 42-43 0.010765078086686789 44-45 0.0017941796811144647 46-47 7.974131916064288E-4 48-49 0.010765078086686789 50-51 3.987065958032144E-4 52-53 0.002591592872720893 54-55 0.010964431384588396 56-57 0.004385772553835358 58-59 7.974131916064288E-4 60-61 0.007376072022359466 62-63 0.005183185745441786 64-65 0.01913791659855429 66-67 0.002591592872720893 68-69 0.0 70-71 9.96766489508036E-4 72-73 0.0 74-75 0.0 76-77 0.0015948263832128576 78-79 0.010765078086686789 80-81 0.01694503032163661 82-83 0.012758611065702861 84-85 0.008771545107670716 86-87 0.011562491278293216 88-89 0.02890622819573304 90-91 0.026513988620913757 92-93 0.0029902994685241076 94-95 0.009170251703473931 96-97 0.010366371490883573 98-99 0.011761844576194823 100-101 0.012359904469899647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 250811.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.526831221006006 #Duplication Level Percentage of deduplicated Percentage of total 1 75.77227349149149 34.49671506479041 2 13.320231141503902 12.128558300080726 3 4.125705374000773 5.63490876689192 4 1.948464597676088 3.548296755140185 5 1.0765551334799404 2.450607193102442 6 0.6944368296475022 1.89693050022074 7 0.4934632387778144 1.572607230992598 8 0.3836602969338149 1.3973470067765472 9 0.30158111489351686 1.2357029265479968 >10 1.6819174365661276 14.289004710113135 >50 0.11865373237001486 3.7120251647387734 >100 0.06328199059734126 5.618455745950113 >500 0.009887811030834573 3.315883745266287 >1k 0.008899029927751115 6.389581031809623 >5k 9.887811030834571E-4 2.3133758575785297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5139 2.0489531958327185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2612 1.041421628237996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2281 0.909449745027132 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1766 0.7041158481884766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 0.570947845190203 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1340 0.5342668383763073 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1337 0.5330707185888977 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1200 0.47844791496385725 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1142 0.45532293240727084 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1084 0.4321979498506844 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 995 0.39671306282419827 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 939 0.3743854934592183 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 923 0.36800618792636686 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 880 0.3508618043068287 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 779 0.31059243813070403 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 682 0.27191789833779223 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 596 0.23762913109871575 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 541 0.21570026832953898 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.2109157891799004 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 502 0.20015071109321364 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 461 0.18380374066528182 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 401 0.15988134491708897 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 390 0.15549557236325362 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 382 0.1523059195968279 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 370 0.14752144044718932 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 347 0.1383511887437154 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 318 0.12678869746542218 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 0.11881456554935788 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 292 0.11642232597453861 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 277 0.11044172703749038 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 268 0.10685336767526145 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 257 0.10246759512142609 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9935329790160718E-4 0.0 22-23 0.0 0.0 0.0 3.9870659580321436E-4 0.0 24-25 0.0 0.0 0.0 0.0011961197874096432 0.0 26-27 0.0 0.0 0.0 0.002591592872720893 0.0 28-29 0.0 0.0 0.0 0.007176718724457859 0.0 30-31 0.0 0.0 0.0 0.014951497342620539 0.0 32-33 0.0 0.0 0.0 0.02551722213140572 0.0 34-35 0.0 0.0 0.0 0.03528553372858447 0.0 36-37 0.0 0.0 0.0 0.043060312346747155 0.0 38-39 0.0 0.0 0.0 0.05442345032713876 0.0 40-41 0.0 0.0 0.0 0.07077042075507055 0.0 42-43 0.0 0.0 0.0 0.10286630171722931 0.0 44-45 0.0 0.0 0.0 0.15350203938423754 0.0 46-47 0.0 0.0 0.0 0.20035006439111522 0.0 48-49 0.0 0.0 0.0 0.28627133578670794 0.0 50-51 0.0 0.0 0.0 0.3749835533529231 0.0 52-53 0.0 0.0 0.0 0.4605061181527126 0.0 54-55 0.0 0.0 0.0 0.6132107443453436 0.0 56-57 0.0 0.0 0.0 0.77648109532676 0.0 58-59 0.0 0.0 0.0 0.9537061771612888 0.0 60-61 0.0 0.0 0.0 1.174988337832073 0.0 62-63 0.0 0.0 0.0 1.501728393092807 0.0 64-65 0.0 0.0 0.0 1.7224124938698862 0.0 66-67 0.0 0.0 0.0 2.022439207211805 0.0 68-69 0.0 0.0 0.0 2.412772964503152 0.0 70-71 0.0 0.0 0.0 2.6513988620913755 0.0 72-73 0.0 0.0 0.0 3.020003109911447 0.0 74-75 0.0 0.0 0.0 3.47432927582921 0.0 76-77 0.0 0.0 0.0 3.8070499300269924 0.0 78-79 0.0 0.0 0.0 4.107475349964714 0.0 80-81 0.0 0.0 0.0 4.5233263293874675 0.0 82-83 0.0 0.0 0.0 4.9780512019010335 0.0 84-85 0.0 0.0 0.0 5.576509802201658 0.0 86-87 0.0 0.0 0.0 6.508685823189573 0.0 88-89 0.0 0.0 0.0 7.473755138331253 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 75 2.3756002E-9 50.66114 2 GGGTAAC 45 9.615826E-4 42.217613 7 GGTAAGG 105 1.020453E-9 40.709843 3 ACCGGGG 25 0.0016150224 37.999638 90-91 GGTGTGC 210 0.0 36.186527 8 AAGGTAA 80 9.953748E-6 36.023365 1 ATGGGTA 125 5.6206773E-9 34.196266 5 TTAGTAC 70 2.0500869E-4 33.92487 3 CCCTGTA 100 1.2949331E-6 33.24637 2 GCTGTAC 75 3.0736043E-4 31.663214 8 ATGGGCG 60 0.0039472226 31.663212 5 ATACAAT 105 1.8954561E-6 31.663212 6 TAAGGTG 185 2.8740033E-10 28.24016 5 ACAGTCC 70 0.008383745 27.139894 8 CGCGGTA 35 0.008391606 27.139894 76-77 ATTACGT 35 0.008391606 27.139894 36-37 ATATGGG 70 0.008383745 27.139894 3 ATATAGG 105 6.889798E-5 27.139894 2 GTAAGGT 160 6.207665E-8 26.715834 4 TGGGAGT 90 8.933664E-4 26.386007 6 >>END_MODULE