##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936557_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 278648 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.664824438000633 31.0 31.0 34.0 30.0 34.0 2 31.691022365134508 33.0 31.0 34.0 30.0 34.0 3 31.724559300623007 33.0 31.0 34.0 30.0 34.0 4 35.36111150986191 37.0 35.0 37.0 33.0 37.0 5 35.35400218196434 37.0 35.0 37.0 33.0 37.0 6 35.51852875312222 37.0 35.0 37.0 33.0 37.0 7 35.39766659010652 37.0 35.0 37.0 33.0 37.0 8 35.34371680399644 37.0 35.0 37.0 33.0 37.0 9 36.86019278803365 39.0 37.0 39.0 33.0 39.0 10-11 36.77012933880738 39.0 37.0 39.0 33.0 39.0 12-13 36.68781042749275 39.0 37.0 39.0 32.0 39.0 14-15 37.69476364445465 40.0 37.0 41.0 32.0 41.0 16-17 37.62693613447791 40.0 37.0 41.0 32.0 41.0 18-19 37.691323820734404 40.0 37.0 41.0 32.0 41.0 20-21 37.65573770491804 40.0 37.0 41.0 32.0 41.0 22-23 37.508525092589935 40.0 37.0 41.0 32.0 41.0 24-25 37.58929007206224 40.0 37.0 41.0 32.0 41.0 26-27 37.54405558267061 40.0 37.0 41.0 32.0 41.0 28-29 37.44065092877035 40.0 37.0 41.0 32.0 41.0 30-31 37.20589417472941 39.0 36.0 41.0 32.0 41.0 32-33 36.92884750653154 39.0 35.5 41.0 31.0 41.0 34-35 36.629787402026935 39.0 35.0 40.5 30.5 41.0 36-37 36.3804836209124 38.5 35.0 40.0 30.0 41.0 38-39 36.12195135080819 38.0 35.0 40.0 30.0 41.0 40-41 35.912988788722686 38.0 35.0 40.0 30.0 41.0 42-43 35.58232967758606 38.0 35.0 40.0 28.5 41.0 44-45 35.143343932129426 38.0 35.0 40.0 26.5 41.0 46-47 34.60478273664265 38.0 34.0 40.0 23.5 41.0 48-49 34.29747028509087 38.0 34.0 40.0 22.5 41.0 50-51 33.3050551233097 37.5 33.0 39.5 20.5 40.5 52-53 33.43066162326663 37.5 33.0 39.5 20.0 40.5 54-55 33.827865263701874 38.0 34.0 40.0 19.0 41.0 56-57 33.64854762998478 38.0 33.5 40.0 16.5 41.0 58-59 33.36819212770233 38.0 33.0 40.0 13.5 41.0 60-61 32.95778365536447 37.0 33.0 40.0 8.5 41.0 62-63 32.646597140478306 37.0 32.0 40.0 7.5 41.0 64-65 32.267873804943875 36.0 32.0 39.5 3.5 41.0 66-67 31.80166913094657 36.0 31.0 39.0 2.0 41.0 68-69 31.349535973701588 35.0 31.0 39.0 2.0 40.0 70-71 30.792390399356893 35.0 30.5 38.0 2.0 40.0 72-73 30.390142760759094 35.0 30.0 37.0 2.0 39.5 74-75 29.97654388332233 35.0 29.5 37.0 2.0 39.0 76-77 29.518031710258104 34.5 29.0 36.0 2.0 39.0 78-79 29.014555998966436 34.0 29.0 36.0 2.0 38.5 80-81 28.479003976342913 34.0 28.0 35.0 2.0 37.0 82-83 27.98936112945365 34.0 26.5 35.0 2.0 37.0 84-85 27.611924363354483 34.0 26.0 35.0 2.0 36.0 86-87 27.33815423042692 34.0 26.0 35.0 2.0 36.0 88-89 26.87464830180012 33.5 25.0 35.0 2.0 35.5 90-91 26.398000344520685 33.0 24.5 35.0 2.0 35.0 92-93 26.00577789899802 33.0 23.5 35.0 2.0 35.0 94-95 25.523902916941804 33.0 20.0 35.0 2.0 35.0 96-97 24.92187455140536 32.5 14.0 35.0 2.0 35.0 98-99 24.237100212454422 32.0 2.0 35.0 2.0 35.0 100-101 21.970896973959977 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3617900964740386 1101 2 0.4967203618758127 1101 3 0.34737986210437555 1101 4 0.6100689791201077 1101 5 0.4335506450218105 1101 6 0.32815477150273864 1101 7 0.32255944964698813 1101 8 0.5126810580766872 1101 9 0.666369204081505 1101 10-11 0.6356947146516347 1101 12-13 0.7503698776843564 1101 14-15 0.879013013181968 1101 16-17 0.6983157178960013 1101 18-19 0.7464123680039236 1101 20-21 0.850446573655006 1101 22-23 0.7969499141296907 1101 24-25 0.8139491431349839 1101 26-27 0.6240306214588642 1101 28-29 0.46456443517735124 1101 30-31 0.3313230711869508 1101 32-33 0.3888032618393993 1101 34-35 0.726154039515805 1101 36-37 0.8830793420436578 1101 38-39 0.817021727836611 1101 40-41 0.7817577590424918 1101 42-43 1.1174050618356262 1101 44-45 1.0709883303594765 1101 46-47 1.268005800876196 1101 48-49 0.9476351789459372 1101 50-51 0.9015004668309032 1101 52-53 0.6790722517049588 1101 54-55 0.7714591146050509 1101 56-57 0.7048961909482472 1101 58-59 0.9928255459925026 1101 60-61 1.0932705688946385 1101 62-63 0.8896267581709978 1101 64-65 0.9922970536775573 1101 66-67 0.9887183647821587 1101 68-69 1.0747025757491038 1101 70-71 1.2652221793265284 1101 72-73 0.9616236253488601 1101 74-75 0.8540345864900942 1101 76-77 1.1368801304824636 1101 78-79 1.0567337582053185 1101 80-81 1.1271250116781601 1101 82-83 1.3387386969005775 1101 84-85 1.615166098434333 1101 86-87 1.3089947731012224 1101 88-89 1.4027928196154065 1101 90-91 1.2021740591103551 1101 92-93 1.3498587587606679 1101 94-95 1.3792878806062028 1101 96-97 1.3355079465556905 1101 98-99 1.1560181653041504 1101 100-101 1.6230271487725467 1102 1 0.1381532404842396 1102 2 0.25691446030520737 1102 3 0.3317960818147121 1102 4 0.3479138052002284 1102 5 0.3997537582837509 1102 6 0.24989082559173426 1102 7 0.20984783693537423 1102 8 0.18062599072749919 1102 9 0.35728361558415145 1102 10-11 0.44457502353194656 1102 12-13 0.15273976534836464 1102 14-15 0.32945327244575395 1102 16-17 0.42940597104507816 1102 18-19 0.44904518828381157 1102 20-21 0.5253863000653212 1102 22-23 0.39499495335120116 1102 24-25 0.14563296011292692 1102 26-27 0.374091768578829 1102 28-29 0.4891237391484111 1102 30-31 0.5104426400124069 1102 32-33 0.7558694230231993 1102 34-35 0.37215807522571964 1102 36-37 0.7548931403280434 1102 38-39 0.7793201607232803 1102 40-41 0.6832999767611767 1102 42-43 0.7128295102012459 1102 44-45 0.8435538014543624 1102 46-47 0.9362739397325726 1102 48-49 1.2938283339626224 1102 50-51 1.7440924932464554 1102 52-53 1.9054236030563132 1102 54-55 1.952050581961224 1102 56-57 2.252667378425315 1102 58-59 2.1391309496508484 1102 60-61 1.8655674294267968 1102 62-63 1.8138982339130578 1102 64-65 1.6243857519427216 1102 66-67 1.7256328811084387 1102 68-69 1.8553001578172719 1102 70-71 1.8436673827717271 1102 72-73 1.7617948372847785 1102 74-75 1.9142230069726338 1102 76-77 2.1677725290219207 1102 78-79 2.071343552226878 1102 80-81 2.070928624599418 1102 82-83 2.2450591252798304 1102 84-85 1.8035822133210395 1102 86-87 1.7504682439864574 1102 88-89 1.8468082636308516 1102 90-91 1.8389645341361245 1102 92-93 1.454245627853421 1102 94-95 1.4047818619825385 1102 96-97 1.0066718383078204 1102 98-99 0.998562147554015 1102 100-101 1.073059032627956 1103 1 -0.04310683987898045 1103 2 0.3601280567923446 1103 3 0.26436369599667486 1103 4 0.07549461125637436 1103 5 0.1320659255734924 1103 6 0.04983582060398817 1103 7 0.03620454894230818 1103 8 0.28916295128239966 1103 9 0.3726943350307437 1103 10-11 0.3421328953050633 1103 12-13 0.16238894540113336 1103 14-15 0.12123142521044628 1103 16-17 0.4518438107474836 1103 18-19 0.2009004412601385 1103 20-21 0.4437738148653878 1103 22-23 0.18605757513130783 1103 24-25 0.35377090143472145 1103 26-27 0.30412822103681236 1103 28-29 -0.2313507021780481 1103 30-31 -0.1494820311230285 1103 32-33 0.34316886458273643 1103 34-35 -0.007854137746136303 1103 36-37 0.2753335531451384 1103 38-39 0.17196953655651015 1103 40-41 -0.12373739866184508 1103 42-43 -0.3733908080818509 1103 44-45 -0.35193532340696265 1103 46-47 -0.06522704383118594 1103 48-49 -0.11427837571359589 1103 50-51 -0.5440613901989053 1103 52-53 -0.3607259545730699 1103 54-55 -0.2525027122781651 1103 56-57 -0.41139898182938595 1103 58-59 -0.3328188373213621 1103 60-61 -0.5599777259389889 1103 62-63 -0.5429909077294468 1103 64-65 -0.01938003868405147 1103 66-67 -0.17660740678207532 1103 68-69 0.06953526218690698 1103 70-71 0.38541756689859596 1103 72-73 0.1997534064092683 1103 74-75 0.3390036862376178 1103 76-77 0.36859434083027764 1103 78-79 0.28618697446940544 1103 80-81 0.29793840429978147 1103 82-83 0.29806435541510723 1103 84-85 0.6706169783378648 1103 86-87 0.7493704749514656 1103 88-89 0.7998139599100895 1103 90-91 0.6253191018808266 1103 92-93 0.5051042650349267 1103 94-95 0.6762035078436419 1103 96-97 0.7402635707784739 1103 98-99 0.8475384954092888 1103 100-101 1.356677335304557 1104 1 0.22125788358888343 1104 2 0.10739263078337657 1104 3 0.15488686490549597 1104 4 0.23607458136100234 1104 5 0.3236234741534645 1104 6 0.18049650819741458 1104 7 0.3410356401231738 1104 8 0.41431691241842117 1104 9 0.4523355906361246 1104 10-11 0.23264709560401542 1104 12-13 0.1356864042950079 1104 14-15 0.2726992876917649 1104 16-17 0.38119807083717916 1104 18-19 0.15568967892829733 1104 20-21 0.10764825432727321 1104 22-23 0.18621602057226738 1104 24-25 0.3152656697456351 1104 26-27 -0.2747536922666285 1104 28-29 0.06316947719415111 1104 30-31 0.26262559219535575 1104 32-33 0.13123463438841299 1104 34-35 -0.03625772519008308 1104 36-37 0.1715667370016405 1104 38-39 0.4487976382007517 1104 40-41 0.8274524369136387 1104 42-43 0.6559036612753957 1104 44-45 0.2531199830205466 1104 46-47 0.11273409219272423 1104 48-49 0.5674166915509744 1104 50-51 0.424186741340705 1104 52-53 0.5274055850382879 1104 54-55 0.760358274268917 1104 56-57 0.540484426242358 1104 58-59 0.2927387112586146 1104 60-61 0.26156188542125136 1104 62-63 0.5685427245575454 1104 64-65 0.4552298267868018 1104 66-67 0.6492281687037256 1104 68-69 0.6775233040404167 1104 70-71 0.6556507507551004 1104 72-73 0.6154275469174912 1104 74-75 0.40949397024359513 1104 76-77 0.9100622033115933 1104 78-79 0.9275292764126029 1104 80-81 0.7983659080367005 1104 82-83 1.0547639069846113 1104 84-85 1.1007963505351768 1104 86-87 1.0627426722608853 1104 88-89 1.219745200567786 1104 90-91 0.6400844232560132 1104 92-93 0.6265422321500225 1104 94-95 0.4064755556762272 1104 96-97 0.5574743331103136 1104 98-99 0.8874159244077937 1104 100-101 1.2040943756633027 1105 1 0.15820492408732534 1105 2 0.27474465570549356 1105 3 0.3547622543135951 1105 4 0.4387163259092901 1105 5 0.4707636610693484 1105 6 0.23156192875816117 1105 7 0.19372099837863033 1105 8 0.4138433921691984 1105 9 0.5005598897015489 1105 10-11 0.36088385572862336 1105 12-13 0.1514994884071541 1105 14-15 -0.18022283068830092 1105 16-17 0.16159682473125514 1105 18-19 0.5144560340684805 1105 20-21 0.2148507465391134 1105 22-23 -0.045572141421494905 1105 24-25 0.3209977569730498 1105 26-27 0.285520451035552 1105 28-29 -0.01270591221394568 1105 30-31 -0.08721864456476425 1105 32-33 0.1214402418650451 1105 34-35 0.09354912839247476 1105 36-37 0.3219717214253137 1105 38-39 0.33779452293595824 1105 40-41 0.35529044314417035 1105 42-43 0.42776658962431213 1105 44-45 -0.1322008892536033 1105 46-47 0.40493346913977035 1105 48-49 1.230855943887427 1105 50-51 1.5404733457020825 1105 52-53 1.3827326878420294 1105 54-55 1.2533925421816932 1105 56-57 1.555936139638007 1105 58-59 1.6063461878941254 1105 60-61 1.575455966418133 1105 62-63 1.6877202946510081 1105 64-65 1.778669079123251 1105 66-67 2.397963371195935 1105 68-69 1.9532366996790493 1105 70-71 1.8565105638392083 1105 72-73 1.658343434767957 1105 74-75 1.6235998892467123 1105 76-77 1.5274204587633058 1105 78-79 1.8796414259453122 1105 80-81 1.8356743192516554 1105 82-83 1.2572935020001879 1105 84-85 1.5311390296629028 1105 86-87 1.5868423607344049 1105 88-89 1.3616268205054816 1105 90-91 1.6112619933494692 1105 92-93 1.874741609097061 1105 94-95 1.360157798666883 1105 96-97 1.2288886633283802 1105 98-99 1.1508053325074812 1105 100-101 1.4768420392147057 1106 1 0.2651288513308181 1106 2 0.30552883150022225 1106 3 0.27617718748614095 1106 4 0.4896659792104643 1106 5 0.4059460547986262 1106 6 0.1833925655450841 1106 7 0.23884209173608184 1106 8 0.40339218123562404 1106 9 0.47570476626336955 1106 10-11 0.4252492461416537 1106 12-13 0.458353070961671 1106 14-15 0.5884268862580697 1106 16-17 0.8195206514823354 1106 18-19 0.5579692488207755 1106 20-21 0.5459564980548706 1106 22-23 0.5948325080274657 1106 24-25 0.33913663748757017 1106 26-27 0.1659344797763822 1106 28-29 0.3167752119611791 1106 30-31 0.3515144810842443 1106 32-33 0.20200165947801452 1106 34-35 0.7061508769604643 1106 36-37 0.6060470237399969 1106 38-39 0.4754356310322976 1106 40-41 0.62732340465557 1106 42-43 0.4284197903076574 1106 44-45 1.095012456138825 1106 46-47 1.1855082857411148 1106 48-49 1.0886926772140555 1106 50-51 1.008315773598774 1106 52-53 1.0630328251816579 1106 54-55 1.1640715359176639 1106 56-57 1.2180471502567016 1106 58-59 1.0745738367066409 1106 60-61 0.9900206667832592 1106 62-63 1.261531971869374 1106 64-65 1.8730649522348308 1106 66-67 1.5759521830406413 1106 68-69 1.8115591463343286 1106 70-71 1.4930127579235588 1106 72-73 1.6721443927956265 1106 74-75 0.9563470168385777 1106 76-77 1.300843565318761 1106 78-79 1.2328052620756864 1106 80-81 1.2092046177141214 1106 82-83 0.8762262725760124 1106 84-85 0.9730544150513047 1106 86-87 1.0407498407196663 1106 88-89 1.4719172435785417 1106 90-91 1.159984064833072 1106 92-93 1.3864992213973366 1106 94-95 1.571593835246123 1106 96-97 1.13767504995619 1106 98-99 1.2664553509310927 1106 100-101 2.525413372079072 1107 1 0.06436290641993381 1107 2 -0.048634766476897795 1107 3 0.03726129412924095 1107 4 -0.25377929991753234 1107 5 -0.16633999616617245 1107 6 0.08203988719284894 1107 7 0.36463381363915914 1107 8 0.0737780996330315 1107 9 0.03781504269092295 1107 10-11 0.10854207277296979 1107 12-13 0.32479142712605125 1107 14-15 0.07814677627624178 1107 16-17 -0.005048504505289486 1107 18-19 0.21708693920226807 1107 20-21 0.5430044187108365 1107 22-23 0.05867720778687868 1107 24-25 0.04248028161777739 1107 26-27 -0.12727880642187728 1107 28-29 -0.3327620296551572 1107 30-31 0.024269427811795197 1107 32-33 0.20296522799571903 1107 34-35 0.23955049398800554 1107 36-37 0.07759413426191486 1107 38-39 -0.22151286408234938 1107 40-41 -0.7549493895703847 1107 42-43 0.014017816526539661 1107 44-45 0.03938938941323755 1107 46-47 0.07046925201007781 1107 48-49 0.2284714860715269 1107 50-51 0.23590820252792355 1107 52-53 0.4718804708830433 1107 54-55 0.4457281372826145 1107 56-57 0.7662378508998984 1107 58-59 0.3054190765554168 1107 60-61 0.5190504698961433 1107 62-63 0.6932413546945355 1107 64-65 1.1101887308963931 1107 66-67 0.7192783970142216 1107 68-69 1.2298338063235228 1107 70-71 1.0917877370564675 1107 72-73 0.9463133916663473 1107 74-75 0.794160636910263 1107 76-77 0.9881809247727809 1107 78-79 0.5546616965039277 1107 80-81 0.5176079171691228 1107 82-83 0.7545036330120105 1107 84-85 0.4493579943707928 1107 86-87 0.3516878777403356 1107 88-89 0.8157086708874246 1107 90-91 1.0254588524797548 1107 92-93 0.8199577572641807 1107 94-95 0.5259869712013447 1107 96-97 0.479214059137707 1107 98-99 0.8279273399329092 1107 100-101 0.6301354715537109 1108 1 -0.07452442933628589 1108 2 -0.22158696105335807 1108 3 -0.10688184257749711 1108 4 0.12135349292562836 1108 5 0.2190248346976844 1108 6 -0.3151225394216297 1108 7 -0.09172626463873002 1108 8 -0.07183274744552648 1108 9 -0.398276654261835 1108 10-11 -0.10716242820550548 1108 12-13 0.14202654034942697 1108 14-15 0.13789656660333094 1108 16-17 0.0536878667555456 1108 18-19 0.053975393214010126 1108 20-21 0.14087274412319317 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0.4132863569637024 2312 24-25 -0.33127867276201073 2312 26-27 0.09367444228994515 2312 28-29 0.8234997524235084 2312 30-31 0.38923109678251677 2312 32-33 0.24093494019881234 2312 34-35 -0.377799009593744 2312 36-37 -0.19889197859469476 2312 38-39 -0.49702499176866155 2312 40-41 0.007366810002324087 2312 42-43 -1.3669831888775121 2312 44-45 -1.3036261943494836 2312 46-47 -2.5794616264617076 2312 48-49 -3.059592482760948 2312 50-51 -3.3457337479559293 2312 52-53 -3.4872121519647656 2312 54-55 -3.2226142027953024 2312 56-57 -3.786671537060389 2312 58-59 -3.7858056312490334 2312 60-61 -3.6814962185542868 2312 62-63 -3.406466449754376 2312 64-65 -3.507192191561824 2312 66-67 -2.6549675499507117 2312 68-69 -2.6987825063571336 2312 70-71 -2.678257506125636 2312 72-73 -3.201355327700245 2312 74-75 -2.831986152080564 2312 76-77 -2.9453995703887124 2312 78-79 -2.716703139489539 2312 80-81 -1.887224306030575 2312 82-83 -1.7989241664098827 2312 84-85 -1.4353504384556501 2312 86-87 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1.2262694083078998 2313 46-47 1.1519524829973236 2313 48-49 1.396244277757873 2313 50-51 1.4347154769728974 2313 52-53 1.274761906877373 2313 54-55 0.8098755156482227 2313 56-57 1.5177258055527076 2313 58-59 1.3639571020632033 2313 60-61 1.212366483122402 2313 62-63 1.2907496211092706 2313 64-65 0.8440574129937026 2313 66-67 0.6902396629565999 2313 68-69 0.7130175569139858 2313 70-71 0.9426852335137177 2313 72-73 0.7863700756531209 2313 74-75 0.37174684380681455 2313 76-77 0.3880392148058469 2313 78-79 0.3656442189413376 2313 80-81 0.4658601609102675 2313 82-83 0.9681432173294411 2313 84-85 0.2201066953627624 2313 86-87 0.0925357757091625 2313 88-89 0.29983715459077587 2313 90-91 0.4510729290031428 2313 92-93 0.6160134965178408 2313 94-95 0.4697399234923232 2313 96-97 1.2256122641447966 2313 98-99 1.1190940853273368 2313 100-101 1.32988747911158 2314 1 -0.026935784939979612 2314 2 -0.06981407312723675 2314 3 -0.25569041628630984 2314 4 -0.03048780225165615 2314 5 0.11189907936761756 2314 6 0.09636987877469494 2314 7 -0.02420830773530014 2314 8 0.08183274109626382 2314 9 0.06976575823389197 2314 10-11 0.042717859104946854 2314 12-13 -0.03885363294894262 2314 14-15 -0.5612597439655858 2314 16-17 -1.2054388006712031 2314 18-19 -1.2279080864348302 2314 20-21 -1.2913014663431142 2314 22-23 -0.7063128434873249 2314 24-25 -0.4026486691742903 2314 26-27 -0.3502546233653234 2314 28-29 -0.44042828816897384 2314 30-31 -0.44240234076553264 2314 32-33 -0.5374208590938991 2314 34-35 -0.26345325591633184 2314 36-37 -0.3411706931659566 2314 38-39 -0.650092492153064 2314 40-41 -0.9356909522856114 2314 42-43 -0.6854445696370846 2314 44-45 -0.43111837824574906 2314 46-47 -1.2529791294087573 2314 48-49 -1.345790012359636 2314 50-51 -1.0171242456239113 2314 52-53 -1.1302852901944824 2314 54-55 -1.1740774799210314 2314 56-57 -1.1604094285062416 2314 58-59 -0.9018683650914809 2314 60-61 -0.8190340177444853 2314 62-63 -0.983166772649156 2314 64-65 -0.8063567653919712 2314 66-67 -0.8983174551324034 2314 68-69 -1.206111705270569 2314 70-71 -1.1347179643287006 2314 72-73 -1.0071571830638852 2314 74-75 -1.0619846144863843 2314 76-77 -0.6979750406362655 2314 78-79 -0.7556811518927979 2314 80-81 -1.140978598480988 2314 82-83 -1.4564763164790762 2314 84-85 -1.3524997388502982 2314 86-87 -1.0307545344430267 2314 88-89 -1.0644857119089366 2314 90-91 -0.8519900646397041 2314 92-93 -0.7701356077010004 2314 94-95 -0.545844742275353 2314 96-97 -0.21360946577236817 2314 98-99 -0.5532619154432297 2314 100-101 -1.0224531103702184 2315 1 0.020788871863590686 2315 2 -0.16557219333722628 2315 3 -0.25027226407272707 2315 4 -0.16403262131905905 2315 5 0.1290238091618363 2315 6 -0.8581232572967252 2315 7 -0.46602633642623914 2315 8 -0.10538828021139324 2315 9 -0.37118199730357304 2315 10-11 -0.4716276112636564 2315 12-13 -0.4260690395910913 2315 14-15 -0.12682500041543676 2315 16-17 -0.0637667532834243 2315 18-19 -0.4371947431822534 2315 20-21 0.027386947794610705 2315 22-23 -0.54699670974599 2315 24-25 -0.015756105798757858 2315 26-27 0.024636592490487885 2315 28-29 0.42063681387756446 2315 30-31 0.5882537328988704 2315 32-33 -0.1692812785094091 2315 34-35 -0.257403453833291 2315 36-37 -0.10571969515915924 2315 38-39 -0.397306214395563 2315 40-41 -0.07179044415838831 2315 42-43 -0.5414564727279583 2315 44-45 0.03098262958670972 2315 46-47 -0.7219861758139743 2315 48-49 -0.61240240392641 2315 50-51 -1.1501918181232753 2315 52-53 -0.5187920699030784 2315 54-55 -0.18227489156023324 2315 56-57 0.16731859709663865 2315 58-59 0.017154121643869757 2315 60-61 -0.2580026036909828 2315 62-63 0.08846232254749964 2315 64-65 -0.30830786165541113 2315 66-67 -0.0261353153833781 2315 68-69 -0.1509055066798517 2315 70-71 0.7619020070365039 2315 72-73 0.8441243643379259 2315 74-75 0.7175274710811195 2315 76-77 1.1795831413350406 2315 78-79 0.8491306164461037 2315 80-81 0.5788215194269881 2315 82-83 0.21520611803988743 2315 84-85 0.3051179585753765 2315 86-87 0.28929543607997843 2315 88-89 0.23516898616242798 2315 90-91 1.114181575684821 2315 92-93 1.2360966710109942 2315 94-95 0.7684923060949878 2315 96-97 0.9889115190399629 2315 98-99 1.2444276497009277 2315 100-101 0.9964963857135523 2316 1 -5.360964338633337 2316 2 -5.079274035883291 2316 3 -5.622890912872283 2316 4 -4.5159254186389965 2316 5 -2.199932081402089 2316 6 -3.431947936247031 2316 7 -6.3958532487657145 2316 8 -6.6799053098038 2316 9 -7.8809977426972075 2316 10-11 -6.8922417932848745 2316 12-13 -8.741646929038328 2316 14-15 -10.59752293453046 2316 16-17 -8.633024151385047 2316 18-19 -8.00697698773837 2316 20-21 -6.864796190117172 2316 22-23 -6.3044506460943985 2316 24-25 -7.77584544136548 2316 26-27 -6.344531470044405 2316 28-29 -5.648386078361405 2316 30-31 -4.810707741137975 2316 32-33 -3.279432032278251 2316 34-35 -3.974924391856746 2316 36-37 -5.3795443741094715 2316 38-39 -4.527646806243691 2316 40-41 -3.6609920075308082 2316 42-43 -2.8116518942801605 2316 44-45 -3.2682133503127915 2316 46-47 -3.291265907807272 2316 48-49 -2.7708055306712467 2316 50-51 -2.5322577031037383 2316 52-53 -3.465927748295041 2316 54-55 -3.85341950350886 2316 56-57 -2.968404462646525 2316 58-59 -2.714816844296948 2316 60-61 -2.1428825590231924 2316 62-63 -2.3554572754424505 2316 64-65 -2.83410350654653 2316 66-67 -2.482327386851832 2316 68-69 -3.3984766959595802 2316 70-71 -2.9416825825782347 2316 72-73 -2.5974613419713997 2316 74-75 -2.404728251978625 2316 76-77 -2.5046044633550757 2316 78-79 -3.164470723587396 2316 80-81 -3.078967425296632 2316 82-83 -2.47545831523761 2316 84-85 -2.454759205020377 2316 86-87 -3.016812883294673 2316 88-89 -3.178477021654434 2316 90-91 -3.1141377989662082 2316 92-93 -3.0614577678499764 2316 94-95 -3.374857777657102 2316 96-97 -4.769636778000798 2316 98-99 -5.291250742258871 2316 100-101 -5.970572291003364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 545.0 3 201.0 4 155.0 5 186.0 6 208.0 7 275.0 8 341.0 9 401.0 10 562.0 11 814.0 12 1208.0 13 1685.0 14 1969.0 15 2717.0 16 3590.0 17 4988.0 18 3871.0 19 2366.0 20 2239.0 21 2407.0 22 2591.0 23 2910.0 24 3161.0 25 3463.0 26 3893.0 27 4409.0 28 5161.0 29 6205.0 30 7386.0 31 9116.0 32 11387.0 33 15029.0 34 19725.0 35 27704.0 36 37504.0 37 46670.0 38 36060.0 39 5546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.151333149046316 18.944466183930412 14.849704362935334 20.05449630408794 2 14.929680601322392 21.714300072509026 39.34727516817069 24.00874415799789 3 20.534486781413214 27.06139957284132 30.639481666277796 21.764631979467666 4 11.515591112132485 16.64284790945521 39.4377576375623 32.403803340850004 5 11.729323848272543 39.01491347455588 34.45684141730078 14.798921259870795 6 28.51351691005124 36.880392670815034 18.108242999177744 16.49784741995598 7 25.723262536131706 35.40889423508501 21.48153467746279 17.38630855132049 8 25.977746900615028 30.62440085737982 21.098796149689612 22.299056092315535 9 26.41503337198907 17.483762794989353 22.40931758860003 33.691886244421546 10-11 24.446283632759975 25.96852115495441 27.041573834534095 22.543621377751528 12-13 27.565519135590815 24.69199409030771 27.338968963344467 20.403517810757005 14-15 23.536465401489203 25.441950469953827 29.156524423956515 21.86505970460045 16-17 20.07256289562217 29.20037197011314 29.056215599047814 21.67084953521688 18-19 19.55621381446075 27.986715138458777 32.36443606660384 20.09263498047664 20-21 21.54879556666601 25.62716134902276 31.318138014080287 21.505905070230945 22-23 20.35474625215592 25.653326441571682 32.49196409822914 21.499963208043262 24-25 21.52564058852136 25.847672813995597 31.960966225879794 20.665720371603253 26-27 20.688108133329024 25.273402029294484 33.733880316848456 20.304609520528032 28-29 19.509608465570487 27.3359903385977 33.06141199313078 20.092989202701027 30-31 19.69969959191877 26.447396283840774 32.42271759845812 21.430186525782336 32-33 19.704095823426496 26.746220447714325 32.49616437127092 21.053519357588264 34-35 21.35632942966762 26.193620862299777 31.705290585621636 20.744759122410965 36-37 20.814580327468757 25.641909109833392 33.01031505049853 20.533195512199324 38-39 20.45082989525141 26.501734444286924 32.41015076116352 20.637284899298148 40-41 19.90891603623261 27.461384418828327 32.42291239000302 20.20678715493605 42-43 19.763640213463106 27.75074198885312 30.327695294696085 22.157922502987695 44-45 20.00200974727428 26.811823056107837 30.783406665183282 22.402760531434602 46-47 21.242441098889266 27.753593281774304 30.207073516481547 20.796892102854887 48-49 21.135788202488126 27.447796198368167 29.63499956037015 21.781416038773557 50-51 19.754814352260574 28.12403551459558 30.738463857367414 21.382686275776432 52-53 20.799814814482513 28.97453519691214 28.390985484828963 21.834664503776384 54-55 19.643430745349974 27.40361234982189 29.928129850786444 23.024827054041687 56-57 21.16825524446032 27.51347344882602 28.621140796867234 22.69713050984643 58-59 21.905624994392053 26.98633455661334 28.777108812102398 22.33093163689221 60-61 20.97472911230668 27.20613306247555 30.849971825526435 20.969165999691334 62-63 20.556895425540315 27.611310895296338 30.287581558603762 21.544212120559585 64-65 19.997631420286528 28.754737159426945 29.785248772645055 21.46238264764147 66-67 20.500590386568614 27.581101887442532 28.750928647566138 23.167379078422716 68-69 19.902551079924265 27.713719120985616 29.848442701650168 22.535287097439944 70-71 21.338269416086295 27.40010444014162 28.805201190832957 22.456424952939127 72-73 20.71192474850972 26.728490986545317 30.21723448619899 22.342349778745977 74-75 19.82435771593252 28.898252011147218 29.136377584254642 22.14101268866562 76-77 20.10117432562413 27.38723315675381 29.279720631092353 23.23187188652971 78-79 20.192801180063814 27.165513998901762 29.985751662605114 22.65593315842931 80-81 20.897236648947818 27.704397888094874 28.861028459034284 22.53733700392303 82-83 20.312099041917673 27.16402965228018 29.925223280377704 22.598648025424446 84-85 20.01539811669188 27.399494984844164 29.011810606753997 23.573296291709962 86-87 20.119760693944492 27.75616478669533 29.177537961304772 22.946536558055406 88-89 20.102895760170693 27.057601168568524 28.35662873723897 24.48287433402182 90-91 19.75677945419831 26.46266096268967 29.211049541337086 24.569510041774933 92-93 20.71209491727402 27.057919852979445 29.575144337501193 22.65484089224535 94-95 19.933242409015865 26.56431699088364 29.30478788313832 24.19765271696217 96-97 18.545143929755174 26.916595932533095 30.566118320110686 23.972141817601045 98-99 19.68433842625081 27.861602824227045 28.146251025254003 24.307807724268145 100-101 19.21552507153294 26.507935938077782 29.24342022596152 25.033118764427755 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 160.0 1 130.5 2 143.5 3 196.0 4 180.0 5 186.5 6 298.5 7 568.5 8 884.5 9 1024.5 10 894.5 11 718.0 12 681.5 13 697.5 14 753.0 15 739.0 16 710.5 17 747.0 18 756.0 19 770.5 20 835.5 21 959.5 22 1072.5 23 1226.0 24 1381.0 25 1600.0 26 1903.5 27 2179.5 28 2505.5 29 2908.0 30 3419.5 31 3966.5 32 4777.5 33 5540.0 34 6050.5 35 6611.0 36 7463.0 37 8048.5 38 8029.5 39 7892.0 40 7850.0 41 8091.0 42 8119.5 43 8354.5 44 8438.0 45 8599.0 46 9210.5 47 12027.5 48 19414.5 49 19093.5 50 11490.5 51 8104.5 52 7028.0 53 6810.0 54 6772.0 55 6145.0 56 5333.0 57 4697.5 58 4228.5 59 3754.0 60 3205.0 61 2884.0 62 2479.0 63 1826.5 64 1254.5 65 972.0 66 733.0 67 564.5 68 407.0 69 245.0 70 194.0 71 168.0 72 136.0 73 125.5 74 96.5 75 78.0 76 61.0 77 36.0 78 28.0 79 19.0 80 12.0 81 11.0 82 7.0 83 3.5 84 1.5 85 1.0 86 2.0 87 1.5 88 1.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03481094427377911 2 0.022250294278085614 3 0.022609169992248282 4 0.0559846114093767 5 0.06280324997846745 6 0.052036978553587326 7 0.054907984266888685 8 0.044859464270333896 9 0.04342396141368322 10-11 0.05149866498234332 12-13 0.04396227498492722 14-15 0.039476328557893826 16-17 0.047012718555309925 18-19 0.048627659269041945 20-21 0.011304584996124141 22-23 0.01955872642186558 24-25 0.006280324997846745 26-27 0.010407395710717464 28-29 0.004844822141196061 30-31 0.009689644282392123 32-33 0.0037681949987080476 34-35 0.007536389997416095 36-37 0.009330768568229451 38-39 0.010227957853636128 40-41 0.0014355028566506848 42-43 0.001794378570813356 44-45 0.002153254284976027 46-47 0.0010766271424880135 48-49 0.0016149407137320204 50-51 7.177514283253424E-4 52-53 0.003050443570382705 54-55 0.007356952140334759 56-57 0.015969969280238867 58-59 0.009151330711148115 60-61 0.009689644282392123 62-63 0.005024259998277396 64-65 0.0 66-67 0.00610088714076541 68-69 0.014534466423588185 70-71 0.006998076426172088 72-73 0.0032298814274640407 74-75 0.004844822141196061 76-77 0.007177514283253424 78-79 0.006818638569090753 80-81 0.013278401424018834 82-83 0.006639200712009417 84-85 0.015252217851913526 86-87 0.0032298814274640407 88-89 0.005921449283684075 90-91 0.004306508569952054 92-93 0.017046596422726882 94-95 0.010048519996554792 96-97 0.008074703568660102 98-99 0.020994229278516265 100-101 0.03768194998708048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 278648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.52258022755463 #Duplication Level Percentage of deduplicated Percentage of total 1 81.55119934642303 43.648306096722266 2 10.228736377170101 10.949367267471862 3 3.1770937218301403 5.101387608513413 4 1.4405824769853945 3.084147647954406 5 0.7839881369758455 2.0980533979370386 6 0.4834161077915573 1.552420644753949 7 0.355312150107913 1.331205614198299 8 0.282430164546457 1.2093112912495356 9 0.2191591648890714 1.0556967586841275 >10 1.3402909376334275 12.939000877312095 >50 0.08401915588236213 3.0802117009046692 >100 0.037640581835298226 3.7677011380021725 >500 0.010082298705883454 3.5543671577830103 >1k 0.006049379223530073 6.6288227985131325 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3042 1.0916999224828459 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2742 0.9840372082340444 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2654 0.9524561453877293 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2301 0.8257730182883064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2072 0.7435904797450547 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1820 0.6531537997760616 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1357 0.4869943441187448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1286 0.46151416841319515 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1152 0.4134248227153972 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 947 0.3398553013120496 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 849 0.30468548132410783 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 826 0.2964313398983664 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 764 0.2741810456202808 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 678 0.24331773420229108 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 671 0.24080560420315236 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 657 0.23578134420487498 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 654 0.23470471706238696 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 604 0.2167609313542534 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 598 0.21460767706927736 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 547 0.19630501564698116 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 528 0.1894863770778904 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 527 0.18912750136372772 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 515 0.18482099279377565 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 515 0.18482099279377565 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 488 0.17513134851138354 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 478 0.17154259136975683 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 426 0.15288105423329792 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 378 0.13565501995348972 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 360 0.12919525709856164 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 348 0.12488874852860958 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 312 0.1119692228187534 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 302 0.1083804656771267 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 298 0.10694496282047602 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.794378570813356E-4 0.0 22-23 0.0 0.0 0.0 0.001256064999569349 0.0 24-25 0.0 0.0 0.0 0.002512129999138698 0.0 26-27 0.0 0.0 0.0 0.004306508569952054 0.0 28-29 0.0 0.0 0.0 0.008792454996985444 0.0 30-31 0.0 0.0 0.0 0.015252217851913524 0.0 32-33 0.0 0.0 0.0 0.022070856421004276 0.0 34-35 0.0 0.0 0.0 0.028710057133013694 0.0 36-37 0.0 0.0 0.0 0.03822026355832448 0.0 38-39 0.0 0.0 0.0 0.04952484855444862 0.0 40-41 0.0 0.0 0.0 0.06639200712009417 0.0 42-43 0.0 0.0 0.0 0.09492262639602653 0.0 44-45 0.0 0.0 0.0 0.13870546352387242 0.0 46-47 0.0 0.0 0.0 0.19271625850535443 0.0 48-49 0.0 0.0 0.0 0.28387068990267295 0.0 50-51 0.0 0.0 0.0 0.36497660130343657 0.0 52-53 0.0 0.0 0.0 0.4349573655651575 0.0 54-55 0.0 0.0 0.0 0.588197295512618 0.0 56-57 0.0 0.0 0.0 0.745384858315868 0.0 58-59 0.0 0.0 0.0 0.9185423903993568 0.0 60-61 0.0 0.0 0.0 1.134585570325285 0.0 62-63 0.0 0.0 0.0 1.4534466423588182 0.0 64-65 0.0 0.0 0.0 1.6651833137147944 0.0 66-67 0.0 0.0 0.0 1.9661006000401942 0.0 68-69 0.0 0.0 0.0 2.3384341534839654 0.0 70-71 0.0 0.0 0.0 2.576189314116735 0.0 72-73 0.0 0.0 0.0 2.939192098992277 0.0 74-75 0.0 0.0 0.0 3.3895811202664294 0.0 76-77 0.0 0.0 0.0 3.7071861273003934 0.0 78-79 0.0 0.0 0.0 3.9783167293502917 0.0 80-81 0.0 0.0 0.0 4.401251758490999 0.0 82-83 0.0 0.0 0.0 4.8566650397634294 0.0 84-85 0.0 0.0 0.0 5.425483046711262 0.0 86-87 0.0 0.0 0.0 6.288399701415406 0.0 88-89 0.0 0.0 0.0 7.194740317533232 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 60 8.2581755E-5 39.596653 1 GTATATG 50 0.0016110479 38.012787 3 TGCACCG 75 6.887496E-6 38.012787 5 CGCTTAC 25 0.0016124331 38.012787 92-93 GATGTGC 55 0.0025720359 34.55708 8 GTGTAAG 105 1.8898681E-6 31.677322 1 AGGGGTC 95 3.473463E-5 30.010096 5 ATATGTG 80 4.4761662E-4 29.697489 5 GTGTTAC 65 0.0058269803 29.240606 1 TAATGTA 115 3.840676E-6 28.922773 7 GGCTTGC 100 4.9285027E-5 28.50959 6 GTACATG 2150 0.0 28.288586 1 GTGTGCA 185 2.8740033E-10 28.25275 9 CATGGGT 175 4.9094524E-9 27.15199 4 CACCGTC 70 0.008367101 27.151989 7 AGTAGGT 70 0.008367101 27.151989 8 TACATGG 2205 0.0 26.721008 2 ATGTATC 125 7.345394E-6 26.608952 9 ACATGGG 2110 0.0 25.897337 3 ACCGAAA 75 1.060338E-6 25.328213 36-37 >>END_MODULE