##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936557_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 278648 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.989068645746606 31.0 31.0 34.0 31.0 34.0 2 32.101159886308174 33.0 31.0 34.0 30.0 34.0 3 32.18019149608108 33.0 31.0 34.0 30.0 34.0 4 35.90882762481697 37.0 35.0 37.0 35.0 37.0 5 35.73798484108983 37.0 35.0 37.0 33.0 37.0 6 35.89441158737906 37.0 35.0 37.0 35.0 37.0 7 35.85115629755103 37.0 35.0 37.0 35.0 37.0 8 35.843257443082315 37.0 35.0 37.0 35.0 37.0 9 37.471752892538255 39.0 37.0 39.0 35.0 39.0 10-11 37.331574244207744 39.0 37.0 39.0 34.0 39.0 12-13 37.251656211420865 39.0 37.0 39.0 34.0 39.0 14-15 38.227222158422094 40.0 37.5 41.0 33.0 41.0 16-17 38.231749375556255 40.0 37.5 41.0 33.5 41.0 18-19 38.47023484826735 40.0 38.0 41.0 34.0 41.0 20-21 38.53913001349373 40.0 38.0 41.0 34.0 41.0 22-23 38.437117797364415 40.0 38.0 41.0 34.0 41.0 24-25 38.27403928971319 40.0 37.5 41.0 34.0 41.0 26-27 37.987003316011595 40.0 37.0 41.0 33.0 41.0 28-29 37.903193276104616 40.0 37.0 41.0 33.0 41.0 30-31 37.809485444001034 40.0 37.0 41.0 33.0 41.0 32-33 37.54635777037696 39.5 36.0 41.0 33.0 41.0 34-35 37.27083811834285 39.0 35.0 41.0 33.0 41.0 36-37 36.94856054951049 39.0 35.0 41.0 31.5 41.0 38-39 36.69897146220321 39.0 35.0 41.0 31.0 41.0 40-41 36.42088405443427 39.0 35.0 40.5 30.5 41.0 42-43 36.06919841520485 39.0 35.0 40.0 30.0 41.0 44-45 35.60236570870776 39.0 35.0 40.0 29.0 41.0 46-47 34.955578364100944 38.0 35.0 40.0 25.0 41.0 48-49 34.32105918578278 38.0 34.0 40.0 22.5 41.0 50-51 33.891827682237086 38.0 34.0 40.0 19.5 41.0 52-53 33.63801642215268 38.0 33.0 40.0 18.0 41.0 54-55 33.38275530418305 38.0 33.0 40.0 14.5 41.0 56-57 33.00390815652723 37.5 33.0 40.0 10.0 41.0 58-59 32.638292756452586 37.0 32.0 40.0 7.5 41.0 60-61 32.177900074646146 36.0 31.5 39.0 4.5 41.0 62-63 31.816569650598606 36.0 31.0 39.0 2.0 40.0 64-65 31.40301383824754 35.0 31.0 39.0 2.0 40.0 66-67 30.89677298957825 35.0 30.5 38.0 2.0 40.0 68-69 30.568150498119493 35.0 30.0 38.0 2.0 40.0 70-71 30.05881972955126 34.5 29.5 37.0 2.0 39.5 72-73 29.57864761275875 34.0 29.0 36.5 2.0 39.0 74-75 29.05600973270937 34.0 28.5 36.0 2.0 39.0 76-77 27.69718067238954 32.5 26.5 34.5 2.0 36.5 78-79 28.426502253739486 34.0 28.0 35.0 2.0 37.0 80-81 28.159423358502483 34.0 28.0 35.0 2.0 37.0 82-83 27.795613103270075 33.5 26.5 35.0 2.0 36.0 84-85 27.356525078234903 33.0 26.0 35.0 2.0 36.0 86-87 27.055582670609514 33.0 26.0 35.0 2.0 35.5 88-89 26.648181576756336 33.0 25.0 35.0 2.0 35.0 90-91 26.114048907582326 33.0 24.0 35.0 2.0 35.0 92-93 25.652026930033593 32.0 22.5 34.0 2.0 35.0 94-95 25.056164049266457 32.0 19.5 34.0 2.0 35.0 96-97 24.27323002497775 31.5 8.5 34.0 2.0 35.0 98-99 23.430561138066665 31.0 2.0 34.0 2.0 35.0 100-101 20.503764606241567 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2936030734060786 1101 2 0.2622727448602049 1101 3 0.36118555194900637 1101 4 0.102555153727387 1101 5 -0.00847477968806487 1101 6 0.17700843704492542 1101 7 0.2328918626783718 1101 8 0.2776244235408285 1101 9 0.33544503235273737 1101 10-11 0.34730122960711896 1101 12-13 0.17647307526110012 1101 14-15 0.42506218720620126 1101 16-17 0.3273137414671865 1101 18-19 0.1405014032294858 1101 20-21 0.12727520231337763 1101 22-23 0.17204619165418933 1101 24-25 0.25479383111939313 1101 26-27 0.150726594527697 1101 28-29 0.32214161337756053 1101 30-31 0.38755241372897586 1101 32-33 0.24028328023204892 1101 34-35 0.09573661779143094 1101 36-37 0.1780318613825429 1101 38-39 0.20063580985814866 1101 40-41 0.18307587400506264 1101 42-43 0.39260332031966527 1101 44-45 0.634293658844534 1101 46-47 0.5428537591597049 1101 48-49 0.4133280007878781 1101 50-51 0.6356550045851677 1101 52-53 0.7140550684080722 1101 54-55 0.9921143112972288 1101 56-57 0.9588742690070831 1101 58-59 0.6207800715066369 1101 60-61 0.5758313045221826 1101 62-63 0.72416873218868 1101 64-65 0.5378350244851227 1101 66-67 0.606085122118305 1101 68-69 0.3818054280799288 1101 70-71 0.726493803717581 1101 72-73 0.7756978073542236 1101 74-75 0.6728271619152366 1101 76-77 0.5422444573845269 1101 78-79 0.26715152336992176 1101 80-81 0.4910794333390385 1101 82-83 0.6726986809280966 1101 84-85 0.5948371166125952 1101 86-87 0.6639161450824282 1101 88-89 0.34610027387823905 1101 90-91 0.48777199392148773 1101 92-93 0.8681689727230619 1101 94-95 1.2240803878617648 1101 96-97 1.1521088378155113 1101 98-99 0.9957356197520291 1101 100-101 1.671334379998875 1102 1 0.33098580196528005 1102 2 0.388310062073991 1102 3 0.42700169129455645 1102 4 0.14888283240800604 1102 5 0.159238667437144 1102 6 0.27067272188568126 1102 7 0.40527432917848216 1102 8 0.2500732783426258 1102 9 0.30922165318818173 1102 10-11 0.312004054604067 1102 12-13 0.23417848590768386 1102 14-15 0.3376676659881497 1102 16-17 0.3975062675420631 1102 18-19 0.4230054651453159 1102 20-21 0.2343787505934003 1102 22-23 0.22502798728304185 1102 24-25 0.34070990232957854 1102 26-27 -0.1083821425222169 1102 28-29 -0.2331572213330375 1102 30-31 0.17273821362066144 1102 32-33 0.5027441897517804 1102 34-35 0.4322991014127382 1102 36-37 0.5646039538369934 1102 38-39 0.40103457643338203 1102 40-41 0.5306186333125282 1102 42-43 0.6873258871010535 1102 44-45 0.7915443969776206 1102 46-47 0.8922527703234238 1102 48-49 0.790524929455394 1102 50-51 0.9558933910000249 1102 52-53 0.9607902489667808 1102 54-55 1.0634380590915669 1102 56-57 0.9876789301646838 1102 58-59 1.338352443490777 1102 60-61 1.1504002173263927 1102 62-63 1.0522077440512163 1102 64-65 1.0677315984993463 1102 66-67 0.7899719824757518 1102 68-69 1.1293748300610957 1102 70-71 1.0883165993639068 1102 72-73 0.7793644507267494 1102 74-75 0.8520690249806293 1102 76-77 0.7393543824356286 1102 78-79 0.8586691075345634 1102 80-81 1.162221486834035 1102 82-83 1.1737425496190248 1102 84-85 0.983942446894396 1102 86-87 1.349700749984681 1102 88-89 0.952756137887043 1102 90-91 1.0307475001322892 1102 92-93 0.9963158655758342 1102 94-95 1.011528631192359 1102 96-97 1.1382131548021803 1102 98-99 0.8332458837328822 1102 100-101 1.12402616357301 1103 1 1.3546214632853548E-4 1103 2 0.07674875948129767 1103 3 0.10794479376232147 1103 4 -0.04700018410281359 1103 5 -0.18565192857452217 1103 6 0.13145882706641032 1103 7 -0.03503593624388657 1103 8 -0.0014952960959035977 1103 9 -0.03074180112504621 1103 10-11 -0.11375785770493252 1103 12-13 0.0015003956062784596 1103 14-15 0.024211488182196206 1103 16-17 -0.0013311694119622075 1103 18-19 0.03891682320914214 1103 20-21 -0.0881975338662997 1103 22-23 -0.11585843207871704 1103 24-25 -0.04106666554564242 1103 26-27 0.08522842216458315 1103 28-29 0.08692286219996248 1103 30-31 -0.07555569947146523 1103 32-33 0.131623672891358 1103 34-35 0.13313722825982666 1103 36-37 0.31895773845983655 1103 38-39 0.4890868404946147 1103 40-41 0.28843819830697726 1103 42-43 0.1514164868418746 1103 44-45 0.08423619191157172 1103 46-47 0.24943325194336552 1103 48-49 0.11379809914112826 1103 50-51 -0.09351087701411132 1103 52-53 -0.3701999340413309 1103 54-55 -0.33824518676208015 1103 56-57 -0.11877203615175347 1103 58-59 0.1120045882191576 1103 60-61 0.2211095952106632 1103 62-63 0.07344992448622634 1103 64-65 -0.1410472907728142 1103 66-67 -0.28785541373729373 1103 68-69 0.1721971453049349 1103 70-71 0.2551471933245466 1103 72-73 0.013182263019899665 1103 74-75 0.13854012871630772 1103 76-77 -0.4170812011300029 1103 78-79 -0.5358075526371628 1103 80-81 -0.266579684119602 1103 82-83 -0.11208294472664804 1103 84-85 -0.17861127619744366 1103 86-87 -0.13468109650478866 1103 88-89 -0.0884486578020578 1103 90-91 0.011253359561916199 1103 92-93 0.21159513242460903 1103 94-95 -0.05299501629092873 1103 96-97 0.02902126197799859 1103 98-99 0.35435754385799356 1103 100-101 0.4113153499653386 1104 1 0.09782156080104087 1104 2 0.21906266082658732 1104 3 -0.02196850967564501 1104 4 -0.08872215719698318 1104 5 -0.1437744995760184 1104 6 0.05417930539226745 1104 7 0.037418473322631485 1104 8 0.136537588806938 1104 9 0.22342262338915475 1104 10-11 0.1957129943130056 1104 12-13 0.030400246129445918 1104 14-15 0.1177092460297331 1104 16-17 0.04783474987055314 1104 18-19 0.04405882619867185 1104 20-21 -0.03336345314880873 1104 22-23 -0.04977173551667846 1104 24-25 -0.20808011846044394 1104 26-27 0.2444660903260214 1104 28-29 0.30372704755122726 1104 30-31 0.11442636330880873 1104 32-33 0.085022776030371 1104 34-35 -0.22161912450549437 1104 36-37 -0.008399511166466311 1104 38-39 -0.12500284560403685 1104 40-41 0.14602265271654602 1104 42-43 0.1262475780227419 1104 44-45 -0.01151866608543628 1104 46-47 -0.16629470022405002 1104 48-49 0.2863392052696767 1104 50-51 0.7199211110277517 1104 52-53 0.7219794381559765 1104 54-55 0.6907921824456906 1104 56-57 0.8378826723684227 1104 58-59 0.7499089230472578 1104 60-61 0.7613607162868945 1104 62-63 0.5815216733651454 1104 64-65 0.43388544465319256 1104 66-67 0.589334421838192 1104 68-69 0.8513152320014967 1104 70-71 0.7871800782273866 1104 72-73 0.6124288997912011 1104 74-75 0.8732277221393261 1104 76-77 0.6322192473004904 1104 78-79 0.7764764533419104 1104 80-81 1.0642783128908633 1104 82-83 1.2064521823286576 1104 84-85 1.3147698897218376 1104 86-87 1.0750618033457364 1104 88-89 1.4623943915252973 1104 90-91 1.6406823580671492 1104 92-93 1.2599000651600356 1104 94-95 1.0912680629317926 1104 96-97 1.404677465266488 1104 98-99 1.3148896813766768 1104 100-101 1.9069506264974727 1105 1 0.030631529648388778 1105 2 0.24562963901973234 1105 3 0.07338351524647635 1105 4 -0.03386857464246873 1105 5 -0.014005029171038075 1105 6 0.08470890040783985 1105 7 0.07589822410144365 1105 8 -0.056284841099603966 1105 9 -0.271979245769721 1105 10-11 -0.05202532344400623 1105 12-13 -0.41837233954033337 1105 14-15 -0.10409760755282349 1105 16-17 0.16955437603876788 1105 18-19 -0.03525581554587376 1105 20-21 0.025963649654933363 1105 22-23 0.12995412118113592 1105 24-25 -0.1162171901115272 1105 26-27 0.2023477102637159 1105 28-29 -9.849762010816221E-5 1105 30-31 0.5420525315331091 1105 32-33 0.6980570439431446 1105 34-35 0.4556020592951313 1105 36-37 0.11206154802355428 1105 38-39 0.2920812665455017 1105 40-41 0.37245255925860477 1105 42-43 0.26765567771121823 1105 44-45 0.9216277513600417 1105 46-47 0.6896180723616254 1105 48-49 0.985342320534464 1105 50-51 1.3955472792520425 1105 52-53 1.44437196152046 1105 54-55 1.6992532416357164 1105 56-57 1.1520010524307267 1105 58-59 0.9627687361313733 1105 60-61 1.2552797194021537 1105 62-63 1.7027054739882033 1105 64-65 1.078994478921107 1105 66-67 1.2122004654518967 1105 68-69 1.1933090014719028 1105 70-71 1.2540959660778555 1105 72-73 1.0975877782958712 1105 74-75 0.6442432984633157 1105 76-77 0.6291413656805531 1105 78-79 0.8727007524073329 1105 80-81 1.0085275340746591 1105 82-83 1.0042714969704 1105 84-85 1.2320658398776025 1105 86-87 0.9825010556821816 1105 88-89 1.0708803728337095 1105 90-91 1.0740406135188643 1105 92-93 1.3610090994279496 1105 94-95 0.7407758510937867 1105 96-97 1.063942262774276 1105 98-99 1.2243476253019132 1105 100-101 1.493492682572242 1106 1 -0.07863722056096734 1106 2 0.05157878985884423 1106 3 -0.01769610824194956 1106 4 -0.13104473784214576 1106 5 -0.23358095118892663 1106 6 -0.39313252256472 1106 7 -0.14950625786016758 1106 8 -0.10612907785973391 1106 9 -0.025236874818730826 1106 10-11 -0.009935751206704424 1106 12-13 -0.07370011229349416 1106 14-15 0.055702077570948916 1106 16-17 0.05366987531857603 1106 18-19 -0.10303794799487065 1106 20-21 -0.4209763563746165 1106 22-23 -0.28630220146650487 1106 24-25 0.008091924550313934 1106 26-27 -0.18198552257720735 1106 28-29 -0.23667438614052827 1106 30-31 -0.17757363669119286 1106 32-33 -0.21026037809149756 1106 34-35 -0.1986370456524469 1106 36-37 -0.7516539914532103 1106 38-39 -0.5514687954248245 1106 40-41 -0.2600131895773572 1106 42-43 -0.3081323502926665 1106 44-45 -0.07080182020730064 1106 46-47 0.19769096285839538 1106 48-49 0.15773705473203847 1106 50-51 0.11857344077684928 1106 52-53 0.46718373923123835 1106 54-55 0.4824911071768696 1106 56-57 0.49511564727881563 1106 58-59 0.5688049803949298 1106 60-61 0.4450596410180765 1106 62-63 0.45822418232572204 1106 64-65 0.3887958389184263 1106 66-67 0.6379365723758248 1106 68-69 0.42737257967174713 1106 70-71 0.233846744894052 1106 72-73 0.23022101448654197 1106 74-75 0.439908725723555 1106 76-77 -0.10366605735900691 1106 78-79 0.4589567400802643 1106 80-81 0.7302137967618307 1106 82-83 0.8440865909308073 1106 84-85 0.8031139757433436 1106 86-87 0.4729542764640122 1106 88-89 0.9286166137475185 1106 90-91 0.9460300283180452 1106 92-93 0.7496635060202514 1106 94-95 1.3321569518206822 1106 96-97 0.948677465266492 1106 98-99 1.5484524053910143 1106 100-101 1.3468072573218457 1107 1 -0.1293565213907435 1107 2 -0.019786654241904955 1107 3 -0.26826074711856407 1107 4 -0.11127010240246449 1107 5 -0.0682767826810462 1107 6 -0.4528983201785124 1107 7 -0.24281896959974603 1107 8 -0.3173162924716024 1107 9 0.17439979233892444 1107 10-11 -0.07362947144041954 1107 12-13 -0.37603354382488874 1107 14-15 -0.3083181512305373 1107 16-17 -0.03454881177329128 1107 18-19 -0.43226537471456794 1107 20-21 -0.26902098739538616 1107 22-23 -0.2643242469322118 1107 24-25 -0.3239157348987902 1107 26-27 -0.652931169947955 1107 28-29 -0.36952409400127806 1107 30-31 -0.7152448695679041 1107 32-33 -0.29625119657237065 1107 34-35 -0.04133601948265664 1107 36-37 0.1703767445412936 1107 38-39 0.13995605850555393 1107 40-41 0.035182470068143346 1107 42-43 -0.31981178270785193 1107 44-45 -0.4844182094644296 1107 46-47 -0.16448191483592467 1107 48-49 -0.023272666876444248 1107 50-51 0.02066996947522881 1107 52-53 0.0575136847313118 1107 54-55 0.18051364363290645 1107 56-57 -0.29922691667267287 1107 58-59 5.573248737462677E-4 1107 60-61 -0.15372147549393134 1107 62-63 0.2075490706254257 1107 64-65 0.6313511980778514 1107 66-67 0.07579560905280047 1107 68-69 0.2733243644215868 1107 70-71 0.18247688188035482 1107 72-73 0.2318535573254472 1107 74-75 -0.16595492626250063 1107 76-77 -0.5944108896858111 1107 78-79 -0.03296646903251954 1107 80-81 0.20802717133834747 1107 82-83 0.19743391748847117 1107 84-85 -0.03384654863432601 1107 86-87 0.33913486270646587 1107 88-89 0.4187231586485858 1107 90-91 0.6261252804415776 1107 92-93 -0.05067527730571797 1107 94-95 0.9255739990048504 1107 96-97 0.6376044059057584 1107 98-99 0.6131956174497368 1107 100-101 0.7250784803787518 1108 1 -0.04895394940304243 1108 2 0.02787898735719807 1108 3 -0.20934265933550833 1108 4 -0.32643644291127316 1108 5 -0.1438289213447277 1108 6 -0.10636491229480782 1108 7 -0.2206495538882578 1108 8 -0.05851003024068291 1108 9 -0.06887669633873372 1108 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-2.2129888237288533 2316 94-95 -1.6945097148459887 2316 96-97 -1.2802114236223971 2316 98-99 -2.022443651956653 2316 100-101 -2.1286049290580813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6.0 5 13.0 6 21.0 7 59.0 8 130.0 9 220.0 10 413.0 11 788.0 12 1108.0 13 1637.0 14 2279.0 15 3094.0 16 4209.0 17 5796.0 18 4734.0 19 2657.0 20 2252.0 21 2223.0 22 2258.0 23 2392.0 24 2730.0 25 3179.0 26 3719.0 27 4458.0 28 5392.0 29 6406.0 30 7804.0 31 9652.0 32 12117.0 33 15432.0 34 20941.0 35 29204.0 36 40205.0 37 49926.0 38 29498.0 39 1696.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.44731646024053 19.614072877400826 15.275534015437083 18.66307664692156 2 16.219174738987967 23.76761763935297 37.631672450982855 22.38153517067621 3 21.956366481361187 27.457749991925034 30.26460761056421 20.32127591614957 4 12.307013199543507 18.149910639305787 38.903483272682905 30.639592888467803 5 11.846973747958872 40.32729817509735 33.29146404923828 14.534264027705504 6 28.43274824419427 38.94389675826404 16.525209315011466 16.098145682530227 7 26.59484367373891 36.42875599322443 20.15517785880394 16.821222474232727 8 26.804785966524076 31.49959805920014 19.442809566191034 22.25280640808475 9 26.920109243727637 18.752310303865517 20.58225040643404 33.74533004597281 10-11 25.10640664924923 27.066944675719906 25.993188538945194 21.83346013608567 12-13 29.041739414417243 25.299529330278077 26.11615834456325 19.542572910741427 14-15 23.880915723703808 26.535028692628202 28.83402777030107 20.750027813366927 16-17 20.13450999673415 29.65859295652829 28.819879342953836 21.387017703783723 18-19 19.524274353305962 28.62033820447303 32.64459102523614 19.21079641698487 20-21 21.98634119031897 25.211916109213057 32.37058941747294 20.431153282995034 22-23 20.77665268828202 25.90110337724233 32.52154627132421 20.800697663151436 24-25 22.475990870070774 26.097996009244774 32.563952570304764 18.8620605503797 26-27 21.49763308725097 25.160336356425837 34.37765806634533 18.96437248997786 28-29 19.398166757587067 28.119526831089036 33.485744638088946 18.996561773234948 30-31 20.202875717538717 26.496580756414556 32.53458271949642 20.765960806550304 32-33 20.48993976238711 27.01505312286356 32.44002705937451 20.05498005537482 34-35 21.885901945301846 25.85851697652717 32.546658531333 19.708922546837986 36-37 21.915681721109358 25.289381969843262 33.4595547196629 19.335381589384482 38-39 20.69297815869803 26.67362946772257 33.4359808128173 19.197411560762102 40-41 20.256741989373594 27.46518425741668 32.556061375475295 19.722012377734433 42-43 20.189343506300087 28.264973245719492 30.56519539058378 20.980487857396636 44-45 21.206052873823097 26.32673532045312 30.860648604225098 21.606563201498684 46-47 22.25388303522724 28.168693118199307 29.885913410467683 19.69151043610577 48-49 22.4916605507715 27.988788622102277 29.06488710565934 20.454663721466883 50-51 20.63876861460171 28.47524901712032 30.624933831696545 20.261048536581427 52-53 21.486826215141015 29.91776141659235 27.817743651766346 20.777668716500294 54-55 20.0369296363023 28.32833281892635 28.839749065095212 22.79498847967614 56-57 22.17237765751281 27.79245201762823 28.558842106774236 21.476328218084724 58-59 23.491043246861143 27.24223876660045 27.817164882134765 21.44955310440364 60-61 21.15981610614451 28.208003904693886 30.555844659218558 20.076335329943046 62-63 21.242687434949577 28.042565409324194 30.052040340236157 20.662706815490075 64-65 20.718720838417916 29.76634843155552 28.941389706410163 20.573541023616396 66-67 21.44933569721287 27.948090353792377 28.09021023693825 22.512363712056505 68-69 20.909746696430496 28.510468780281222 28.660125323896956 21.919659199391326 70-71 22.398151072320633 28.597370158766616 28.13065229249806 20.87382647641469 72-73 21.76540675557342 27.400195231191056 29.520283946541102 21.31411406669442 74-75 20.52894329776658 30.244839949610775 27.94968973301607 21.276527019606576 76-77 21.066146519331465 28.235817670893166 28.131740352212002 22.56629545756337 78-79 21.069065016724327 28.14334831105815 28.423103977949726 22.36448269426779 80-81 21.819483623678373 28.467272479309774 27.928752413560442 21.78449148345141 82-83 21.560401028591322 28.130142283680893 28.41636055965991 21.893096128067874 84-85 20.57097123251044 28.696068807973283 28.281720773569297 22.45123918594698 86-87 20.67826963900867 28.89097046140926 27.2879801748162 23.14277972476587 88-89 20.350696475807293 28.905450851886282 26.641410969179912 24.102441703126516 90-91 21.022416277688347 27.16531264524998 27.625805548526728 24.18646552853495 92-93 21.711036304388394 28.18183775713814 27.636877162257218 22.47024877621625 94-95 21.259151593453918 27.96063020384726 27.40974016652311 23.370478036175708 96-97 19.866855676577725 28.13472339356529 28.881013475928153 23.117407453928834 98-99 20.854874164460995 29.381544120945673 25.85456013637791 23.909021578215423 100-101 19.817288177565313 28.603139226695806 26.267830193927193 25.31174240181169 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 472.0 1 327.5 2 213.5 3 241.5 4 233.5 5 242.5 6 334.0 7 563.0 8 827.0 9 993.5 10 938.0 11 783.0 12 757.0 13 795.5 14 850.5 15 866.5 16 849.0 17 882.0 18 934.0 19 973.5 20 1005.0 21 1079.5 22 1173.0 23 1255.0 24 1388.0 25 1585.5 26 1819.5 27 2117.0 28 2435.0 29 2881.0 30 3438.0 31 3974.5 32 4706.5 33 5336.5 34 5765.5 35 6288.5 36 7176.0 37 7780.5 38 7931.0 39 7947.5 40 7746.5 41 7892.5 42 8083.0 43 8231.5 44 8300.0 45 8307.5 46 8468.0 47 12274.0 48 20119.0 49 18491.5 50 10889.5 51 8086.0 52 7081.0 53 6867.5 54 6512.0 55 5924.5 56 5281.5 57 4740.0 58 4511.5 59 4150.5 60 3534.0 61 3025.5 62 2467.0 63 1875.5 64 1348.0 65 993.5 66 767.5 67 665.0 68 475.5 69 287.5 70 239.5 71 187.5 72 161.5 73 156.5 74 135.0 75 113.5 76 91.5 77 64.0 78 44.5 79 31.0 80 29.5 81 29.0 82 20.5 83 11.5 84 6.5 85 3.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035169819987941775 2 0.007536389997416095 3 0.0032298814274640407 4 7.177514283253424E-4 5 0.0010766271424880135 6 3.588757141626712E-4 7 0.0 8 0.0 9 0.001794378570813356 10-11 0.0 12-13 8.97189285406678E-4 14-15 0.001794378570813356 16-17 0.002512129999138698 18-19 0.0 20-21 0.0 22-23 0.001256064999569349 24-25 0.0014355028566506848 26-27 0.005383135712440068 28-29 0.005742011426602739 30-31 0.0016149407137320204 32-33 5.383135712440067E-4 34-35 0.010048519996554792 36-37 0.013278401424018834 38-39 0.008792454996985444 40-41 8.97189285406678E-4 42-43 3.588757141626712E-4 44-45 0.001256064999569349 46-47 0.0 48-49 8.97189285406678E-4 50-51 8.97189285406678E-4 52-53 0.002691567856220034 54-55 0.003588757141626712 56-57 0.0014355028566506848 58-59 0.001256064999569349 60-61 0.0032298814274640407 62-63 0.006459762854928081 64-65 0.010048519996554792 66-67 0.003588757141626712 68-69 0.003588757141626712 70-71 0.0 72-73 0.0014355028566506848 74-75 0.00610088714076541 76-77 0.0032298814274640407 78-79 0.004306508569952054 80-81 0.005024259998277396 82-83 0.005203697855358732 84-85 0.006280324997846745 86-87 0.00448594642703339 88-89 0.011125147139042807 90-91 0.012022336424449484 92-93 0.0014355028566506848 94-95 0.0028710057133013696 96-97 0.0010766271424880135 98-99 0.0037681949987080476 100-101 0.00448594642703339 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 278648.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.010890341493266 #Duplication Level Percentage of deduplicated Percentage of total 1 79.76010405025772 39.88873817283532 2 11.086771917692147 11.089186692336977 3 3.5646257774943146 5.34810326600185 4 1.604142328588466 3.208983443487417 5 0.9084522328936661 2.2716252499864917 6 0.5938457238741948 1.7819252025862227 7 0.3828297477231224 1.3401959572989823 8 0.3065963423395855 1.2266524844678348 9 0.2196917292962219 0.9888281084489741 >10 1.4092571989086664 13.362117768471512 >50 0.09691753673597515 3.4718396562287204 >100 0.052407260605379154 5.321213603409086 >500 0.004307446077154452 1.4393593776443576 >1k 0.009332799833834645 7.264294908425614 >5k 7.179076795257418E-4 1.9969361083706454 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5562 1.9960667221727773 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2934 1.0529413453532772 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2568 0.9215928339697396 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1927 0.6915535011914674 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1593 0.5716890126611353 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1492 0.5354425655307053 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1477 0.5300594298182654 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1337 0.4798168298354914 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1288 0.4622319198415205 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1166 0.4184490827136746 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1161 0.41665470414286127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1108 0.39763429129223965 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1095 0.3929689070081249 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1087 0.3900979012948236 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 779 0.2795641813327209 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 739 0.26520915276621404 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 716 0.25695501134047255 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 635 0.2278860784932962 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 614 0.22034968849588013 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 526 0.18876862564956504 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 497 0.17836122993884757 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 454 0.1629295742298527 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 450 0.16149407137320204 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 448 0.16077631994487668 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 445 0.15969969280238866 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 410 0.14713904280669518 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 391 0.14032040423760445 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 331 0.11878786138784417 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 323 0.1159168556745428 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 320 0.11484022853205478 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 318 0.11412247710372943 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.10227957853636128 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 282 0.10120295139387327 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.97189285406678E-4 0.0 24-25 0.0 0.0 0.0 0.002153254284976027 0.0 26-27 0.0 0.0 0.0 0.003947632855789383 0.0 28-29 0.0 0.0 0.0 0.008433579282822772 0.0 30-31 0.0 0.0 0.0 0.014534466423588181 0.0 32-33 0.0 0.0 0.0 0.021353104992678937 0.0 34-35 0.0 0.0 0.0 0.02799230570468835 0.0 36-37 0.0 0.0 0.0 0.03804082570124315 0.0 38-39 0.0 0.0 0.0 0.05024259998277397 0.0 40-41 0.0 0.0 0.0 0.06764807211966352 0.0 42-43 0.0 0.0 0.0 0.09564037782435186 0.0 44-45 0.0 0.0 0.0 0.13924377709511643 0.0 46-47 0.0 0.0 0.0 0.19522838850449314 0.0 48-49 0.0 0.0 0.0 0.28656225775889294 0.0 50-51 0.0 0.0 0.0 0.3676681691596566 0.0 52-53 0.0 0.0 0.0 0.43639286842180813 0.0 54-55 0.0 0.0 0.0 0.5889150469409434 0.0 56-57 0.0 0.0 0.0 0.746461485458356 0.0 58-59 0.0 0.0 0.0 0.9203367689701702 0.0 60-61 0.0 0.0 0.0 1.136738824610261 0.0 62-63 0.0 0.0 0.0 1.4550615830725504 0.0 64-65 0.0 0.0 0.0 1.667157130142689 0.0 66-67 0.0 0.0 0.0 1.971483735752634 0.0 68-69 0.0 0.0 0.0 2.3495593006230084 0.0 70-71 0.0 0.0 0.0 2.5898265912549165 0.0 72-73 0.0 0.0 0.0 2.95570038184376 0.0 74-75 0.0 0.0 0.0 3.407524905974563 0.0 76-77 0.0 0.0 0.0 3.728359794435991 0.0 78-79 0.0 0.0 0.0 4.002899715770434 0.0 80-81 0.0 0.0 0.0 4.428526312767362 0.0 82-83 0.0 0.0 0.0 4.886990037610175 0.0 84-85 0.0 0.0 0.0 5.455090293129683 0.0 86-87 0.0 0.0 0.0 6.3203396399758835 0.0 88-89 0.0 0.0 0.0 7.2243475639516515 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACT 40 7.533823E-6 59.37063 4 GTAAGCG 45 5.694237E-7 36.941723 94-95 TAAGGTG 140 4.5292836E-10 33.92607 5 GTAAGGT 100 1.2952605E-6 33.24755 4 TATAGGG 60 0.003947283 31.664337 2 TAAGGGT 60 0.003947283 31.664337 4 CTAGATC 75 3.0738363E-4 31.664333 4 TGGGGCG 95 3.4832083E-5 29.99779 5 TTAGAAC 80 4.4869373E-4 29.685314 3 TAGGGGC 65 0.0058386754 29.228615 4 GGTAAGG 115 3.8529724E-6 28.910913 3 GGTGTGC 215 1.8189894E-12 28.718815 8 ATAAGAC 85 6.397662E-4 27.93912 3 AGGTAAG 120 5.365575E-6 27.706295 2 CTAGACA 70 0.008383865 27.140858 4 GACGAAT 35 0.008390939 27.140858 72-73 ACGAGTC 35 0.008390939 27.140858 66-67 TAAGCCT 70 0.008383865 27.140858 4 ATACACT 70 0.008383865 27.140858 4 GGACCAT 90 8.934318E-4 26.386944 6 >>END_MODULE