##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936556_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302989 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.635561026967977 31.0 31.0 34.0 30.0 34.0 2 31.67907745825756 33.0 31.0 34.0 30.0 34.0 3 31.61403219258785 33.0 31.0 34.0 30.0 34.0 4 35.30821250936503 37.0 35.0 37.0 33.0 37.0 5 35.34658683978626 37.0 35.0 37.0 33.0 37.0 6 35.43459003462172 37.0 35.0 37.0 33.0 37.0 7 35.3260910462096 37.0 35.0 37.0 33.0 37.0 8 35.325800606622686 37.0 35.0 37.0 33.0 37.0 9 36.826706580106865 39.0 37.0 39.0 33.0 39.0 10-11 36.735642218034315 39.0 37.0 39.0 32.0 39.0 12-13 36.664514223288634 39.0 37.0 39.0 32.0 39.0 14-15 37.60818049500147 40.0 37.0 41.0 32.0 41.0 16-17 37.58734970576489 40.0 37.0 41.0 32.0 41.0 18-19 37.57158015637532 40.0 37.0 41.0 32.0 41.0 20-21 37.60446748891874 40.0 37.0 41.0 32.0 41.0 22-23 37.56194614325932 40.0 37.0 41.0 32.0 41.0 24-25 37.634006515088004 40.0 37.0 41.0 32.0 41.0 26-27 37.518074913610725 40.0 37.0 41.0 32.0 41.0 28-29 37.400271957067744 39.5 37.0 41.0 32.0 41.0 30-31 37.158441395562214 39.0 36.0 41.0 31.5 41.0 32-33 36.894968134156684 39.0 35.5 41.0 31.0 41.0 34-35 36.65305836185472 39.0 35.0 40.5 30.5 41.0 36-37 36.378817382809274 39.0 35.0 40.0 30.0 41.0 38-39 36.12722409064355 38.0 35.0 40.0 30.0 41.0 40-41 35.88468393241999 38.0 35.0 40.0 29.5 41.0 42-43 35.506944146487164 38.0 35.0 40.0 28.5 41.0 44-45 35.17870780787422 38.0 35.0 40.0 26.5 41.0 46-47 34.77149995544393 38.0 34.5 40.0 24.0 41.0 48-49 34.38019202017235 38.0 34.0 40.0 23.0 41.0 50-51 33.32882381868649 37.5 32.5 39.5 21.0 40.5 52-53 33.398646485515975 37.5 33.0 39.5 20.0 40.5 54-55 33.82830069738505 38.0 34.0 40.0 19.5 41.0 56-57 33.59066005696576 38.0 33.5 40.0 17.0 41.0 58-59 33.35740241394902 38.0 33.0 40.0 14.0 41.0 60-61 32.97891342589995 37.0 33.0 40.0 10.0 41.0 62-63 32.665512609368655 37.0 32.0 40.0 8.0 41.0 64-65 32.277543079121685 36.0 32.0 39.5 4.5 41.0 66-67 31.83375964143913 36.0 31.0 39.0 2.0 41.0 68-69 31.366262471574878 35.0 31.0 39.0 2.0 40.0 70-71 30.860376119265055 35.0 30.5 38.0 2.0 40.0 72-73 30.434177478390303 35.0 30.0 37.0 2.0 39.5 74-75 30.018682196383367 35.0 30.0 37.0 2.0 39.0 76-77 29.55846746911604 34.5 29.0 36.0 2.0 39.0 78-79 29.04915029918578 34.0 29.0 36.0 2.0 38.5 80-81 28.543245794401777 34.0 28.0 35.0 2.0 37.0 82-83 28.1164860770523 34.0 27.0 35.0 2.0 37.0 84-85 27.725975200419818 34.0 26.0 35.0 2.0 36.0 86-87 27.437689157032104 34.0 26.0 35.0 2.0 36.0 88-89 27.041597219701046 34.0 25.5 35.0 2.0 36.0 90-91 26.641680060992314 33.0 25.0 35.0 2.0 35.0 92-93 26.176633475142662 33.0 24.0 35.0 2.0 35.0 94-95 25.66728165048896 33.0 21.5 35.0 2.0 35.0 96-97 25.065198736587796 32.5 18.0 35.0 2.0 35.0 98-99 24.365637036328053 32.0 2.0 35.0 2.0 35.0 100-101 22.037945271940565 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35313702534822156 1101 2 0.35509575976649543 1101 3 0.4884053885897899 1101 4 0.4063757638136849 1101 5 0.18300445530145026 1101 6 0.34632363863040183 1101 7 0.3819939889996036 1101 8 0.1737370041573314 1101 9 0.4522989188203539 1101 10-11 0.5640484276042201 1101 12-13 0.6060324895529661 1101 14-15 0.5733800979887462 1101 16-17 0.8530433641531516 1101 18-19 0.9237663022264542 1101 20-21 0.758390247346135 1101 22-23 0.607480763307727 1101 24-25 0.6514681779716511 1101 26-27 0.5624208177639076 1101 28-29 0.5426227477223478 1101 30-31 0.4401823678214356 1101 32-33 0.6025184693683059 1101 34-35 0.6298319318333583 1101 36-37 0.7392725557481654 1101 38-39 0.882588532805066 1101 40-41 0.7704496647043939 1101 42-43 0.9853583398127199 1101 44-45 0.8059939568015082 1101 46-47 0.7706085749237914 1101 48-49 0.845888309444824 1101 50-51 1.2129984596317627 1101 52-53 1.1601285738547062 1101 54-55 1.2469766586745763 1101 56-57 1.221860675338057 1101 58-59 1.4013958840229321 1101 60-61 1.4501000564387994 1101 62-63 1.2123851496804576 1101 64-65 1.0024054545789518 1101 66-67 1.184213283269635 1101 68-69 1.4135680178400953 1101 70-71 1.2928332060535581 1101 72-73 1.6368429606742367 1101 74-75 1.4301194678607025 1101 76-77 1.3875938378729202 1101 78-79 1.5992396810535645 1101 80-81 1.5124296779720048 1101 82-83 1.8210814085112617 1101 84-85 1.646586840146746 1101 86-87 1.6025947208346096 1101 88-89 1.805663665723685 1101 90-91 1.7361428324400023 1101 92-93 1.8852181253861744 1101 94-95 1.871317509233549 1101 96-97 2.1693257144738887 1101 98-99 1.6092149827011788 1101 100-101 1.5841462140008105 1102 1 0.18591082965902928 1102 2 0.16844511172335075 1102 3 0.3936073242982232 1102 4 0.3796718201633098 1102 5 0.1082184424042083 1102 6 0.1822487702787896 1102 7 0.4108162833305826 1102 8 0.12004841667805266 1102 9 0.06041527091568355 1102 10-11 0.36283692563370096 1102 12-13 0.45333159243519816 1102 14-15 0.5828939627062368 1102 16-17 0.24775272718831332 1102 18-19 0.18624903916547453 1102 20-21 0.1686429149334927 1102 22-23 -0.06119571663717949 1102 24-25 0.21626687851695436 1102 26-27 0.0016155450917594294 1102 28-29 -0.11380210509719291 1102 30-31 -0.2472542407215812 1102 32-33 0.06125367179055985 1102 34-35 -0.20616243919238997 1102 36-37 -0.14584060385046627 1102 38-39 -0.25864482635268615 1102 40-41 0.14763959393052062 1102 42-43 0.14191980005247729 1102 44-45 0.42164991569203636 1102 46-47 0.7398943613895455 1102 48-49 1.0344552040400359 1102 50-51 0.9779812994941821 1102 52-53 0.7355489691554098 1102 54-55 0.8452909605358059 1102 56-57 0.6175375183218677 1102 58-59 0.7873492728226239 1102 60-61 0.7988363442350845 1102 62-63 0.5826436558315393 1102 64-65 0.617455101083852 1102 66-67 0.7932147765945814 1102 68-69 0.855534191597247 1102 70-71 0.5451040704978283 1102 72-73 0.5259720041603089 1102 74-75 0.39982069059068337 1102 76-77 0.25800205078587624 1102 78-79 0.7294733358821723 1102 80-81 1.0120516683982572 1102 82-83 0.8522522276692328 1102 84-85 1.0239882301740302 1102 86-87 0.9771329690409374 1102 88-89 1.2698014109152922 1102 90-91 0.918577363801699 1102 92-93 1.2549489152016235 1102 94-95 1.5354223189375418 1102 96-97 1.325423083751815 1102 98-99 1.3627527406178572 1102 100-101 1.8265588881752741 1103 1 0.20432108606928523 1103 2 0.41068968766023417 1103 3 0.46553631838106213 1103 4 0.6953207353506912 1103 5 0.43682988224642116 1103 6 0.4336215513438759 1103 7 0.48934882770928567 1103 8 0.16474269675695297 1103 9 0.2005493932036515 1103 10-11 0.32276000255678383 1103 12-13 0.14100023148845509 1103 14-15 0.8112036274005163 1103 16-17 0.5551610112119789 1103 18-19 0.737828920822281 1103 20-21 0.5594851240064713 1103 22-23 0.4154295299111439 1103 24-25 0.49182111914812054 1103 26-27 0.34783448000300154 1103 28-29 0.48199276669768665 1103 30-31 0.5817003943869068 1103 32-33 0.7575810879641267 1103 34-35 0.4572892373362052 1103 36-37 0.6548911515735938 1103 38-39 0.7198541874540183 1103 40-41 0.48868495882203433 1103 42-43 0.5765253227349163 1103 44-45 0.7941153989267562 1103 46-47 1.177213888016766 1103 48-49 1.079717531456211 1103 50-51 0.8976834691194284 1103 52-53 0.9862215529818386 1103 54-55 0.9837091064924124 1103 56-57 1.2314527056985938 1103 58-59 1.1244774779508333 1103 60-61 1.0766351608031286 1103 62-63 1.2993320187506683 1103 64-65 1.265896915679516 1103 66-67 1.2380254274821567 1103 68-69 0.969725513096261 1103 70-71 1.4427667923913177 1103 72-73 1.2894217083023172 1103 74-75 1.3901934716557705 1103 76-77 1.4717456405294662 1103 78-79 1.452816531148443 1103 80-81 1.5270122206665029 1103 82-83 1.5439011428566118 1103 84-85 1.4384616029550976 1103 86-87 1.6080501288042512 1103 88-89 1.5327718251164768 1103 90-91 1.8714274624210283 1103 92-93 1.6585426035645447 1103 94-95 1.6742605832373414 1103 96-97 1.9462574032784445 1103 98-99 2.117032819513323 1103 100-101 2.4439849236782294 1104 1 -0.0455376335775135 1104 2 0.12763891503330171 1104 3 0.31195574442962837 1104 4 0.4381772474919714 1104 5 0.4344590213192703 1104 6 0.42162596635491667 1104 7 0.12805964448632778 1104 8 0.3299965598916117 1104 9 0.35200193183499806 1104 10-11 0.4110624308128479 1104 12-13 0.4924527701198045 1104 14-15 0.5913647753033828 1104 16-17 0.6261897087837269 1104 18-19 0.3644757199392785 1104 20-21 0.49259108427137477 1104 22-23 0.47156418774778786 1104 24-25 0.7236665937618127 1104 26-27 0.23700225042242806 1104 28-29 0.16748504042836743 1104 30-31 0.10792555994981257 1104 32-33 0.18119698200386836 1104 34-35 0.7283046898748395 1104 36-37 0.5678955665846317 1104 38-39 0.4546864170345941 1104 40-41 0.5816430162171855 1104 42-43 0.7514590975693594 1104 44-45 0.48758118259120664 1104 46-47 1.1625141087673185 1104 48-49 1.214393028144947 1104 50-51 1.1645024536668913 1104 52-53 0.9555615088317353 1104 54-55 0.7339033669780619 1104 56-57 1.1891900125418573 1104 58-59 1.0498726214386878 1104 60-61 0.8603526000967321 1104 62-63 0.9070163454614857 1104 64-65 1.1156982401828266 1104 66-67 1.0508267519854684 1104 68-69 1.0244606124340088 1104 70-71 1.1401508983515853 1104 72-73 1.4588455493619215 1104 74-75 1.0972552818102947 1104 76-77 1.0541474065338825 1104 78-79 0.9063154273956826 1104 80-81 1.2219018453905655 1104 82-83 1.0178216726579379 1104 84-85 1.0466249583634841 1104 86-87 0.5735578550735667 1104 88-89 0.88386674785378 1104 90-91 0.9586128928846023 1104 92-93 1.0558097117323157 1104 94-95 0.8793621284912092 1104 96-97 0.8361536505190585 1104 98-99 0.2126619585861711 1104 100-101 1.1211041289762456 1105 1 0.12741723991543807 1105 2 0.24197814919869387 1105 3 0.41413247222721594 1105 4 0.2371668891968426 1105 5 0.16344526686180672 1105 6 0.03302539749772393 1105 7 0.4551949815554366 1105 8 0.217435004449257 1105 9 0.4447224701267345 1105 10-11 0.24865423332600756 1105 12-13 0.4553656161038404 1105 14-15 0.8401363197082077 1105 16-17 0.5425937035196711 1105 18-19 0.4429058438991973 1105 20-21 0.3668985855449307 1105 22-23 0.46660260683422194 1105 24-25 0.01699419607120234 1105 26-27 0.11080563384915365 1105 28-29 0.1666466128515296 1105 30-31 0.17030616361767414 1105 32-33 0.2334368571949028 1105 34-35 0.2492123142592888 1105 36-37 0.23939115157359225 1105 38-39 0.06645995668479543 1105 40-41 0.20363688189895868 1105 42-43 -0.35583044649585105 1105 44-45 0.08998078354213845 1105 46-47 0.4395985034013776 1105 48-49 0.867880992994678 1105 50-51 0.705298853734817 1105 52-53 0.8830773222126069 1105 54-55 0.9864398757231854 1105 56-57 0.7171738595447508 1105 58-59 0.8939197856431349 1105 60-61 0.707952468495435 1105 62-63 0.7135916341352839 1105 64-65 0.6243584541410563 1105 66-67 0.5931408120975448 1105 68-69 0.7311197438654915 1105 70-71 0.7754110231605509 1105 72-73 0.6147294006100132 1105 74-75 0.5871357793480776 1105 76-77 0.7467841020679273 1105 78-79 0.8490184542253658 1105 80-81 1.0366756822049616 1105 82-83 0.8311415274719991 1105 84-85 0.9748942952627893 1105 86-87 1.094828974958098 1105 88-89 1.165973748193398 1105 90-91 1.1037351547287209 1105 92-93 0.8026868343337732 1105 94-95 1.1170298140065746 1105 96-97 1.2651420186630595 1105 98-99 0.914003973359474 1105 100-101 1.074802231370537 1106 1 0.08232108606928534 1106 2 -0.055810312339765744 1106 3 0.12153631838106094 1106 4 0.3533207353506924 1106 5 0.3238298822464216 1106 6 0.09562155134387496 1106 7 0.3538488277092853 1106 8 0.3022426967569487 1106 9 0.4165493932036526 1106 10-11 0.6410100025567829 1106 12-13 0.4870002314884587 1106 14-15 0.6747036274005183 1106 16-17 0.4246610112119811 1106 18-19 0.47982892082227835 1106 20-21 0.2722351240064711 1106 22-23 -0.2340704700888594 1106 24-25 -0.31367888085187445 1106 26-27 -0.3349155199970042 1106 28-29 0.02549276669768119 1106 30-31 0.08795039438690111 1106 32-33 -0.06891891203586908 1106 34-35 0.033539237336206895 1106 36-37 -0.27435884842640945 1106 38-39 0.06285418745402183 1106 40-41 -0.2950650411779634 1106 42-43 -0.3334746772650803 1106 44-45 -0.3683846010732523 1106 46-47 0.03921388801676784 1106 48-49 -0.1642824685437887 1106 50-51 -0.2968165308805695 1106 52-53 -0.4205284470181567 1106 54-55 -0.006540893507590795 1106 56-57 0.053952705698591785 1106 58-59 0.009977477950826596 1106 60-61 0.10213516080312957 1106 62-63 -0.04241798124932927 1106 64-65 -0.20160308432048524 1106 66-67 0.26102542748215996 1106 68-69 0.5247255130962607 1106 70-71 0.19401679239131653 1106 72-73 0.2311717083023197 1106 74-75 0.46569347165577213 1106 76-77 0.3514956405294676 1106 78-79 0.39156653114844175 1106 80-81 0.7920122206665035 1106 82-83 0.9271511428566122 1106 84-85 0.7707116029550924 1106 86-87 0.8018001288042527 1106 88-89 0.9997718251164756 1106 90-91 0.9801774624210289 1106 92-93 1.2000426035645404 1106 94-95 0.8962605832373463 1106 96-97 1.1722574032784436 1106 98-99 1.2970328195133227 1106 100-101 2.1314849236782294 1107 1 -0.014965346091518228 1107 2 -0.06833543796790664 1107 3 -0.09162951076466541 1107 4 -0.023463184247297875 1107 5 0.04767410335194455 1107 6 -0.05292116222396004 1107 7 -0.06046021751684805 1107 8 0.0184110384654943 1107 9 0.04509210677149156 1107 10-11 -0.10044100246834375 1107 12-13 -0.09460780871255281 1107 14-15 -0.028600392699985377 1107 16-17 0.1345981971416279 1107 18-19 0.19633645851071435 1107 20-21 -0.24201738855634147 1107 22-23 -0.37649509320443286 1107 24-25 -0.06668139341469015 1107 26-27 -0.40257255517288115 1107 28-29 -0.5761780875736733 1107 30-31 -0.5814151835025498 1107 32-33 0.05096802263749112 1107 34-35 -0.13612784809092915 1107 36-37 -0.28479226551183245 1107 38-39 0.09049237840879698 1107 40-41 0.11496636585721376 1107 42-43 -0.18184151143593397 1107 44-45 -0.32588460107324835 1107 46-47 -0.08156500645559817 1107 48-49 0.48556677768736733 1107 50-51 0.5329950269083739 1107 52-53 0.49834718112253995 1107 54-55 0.5648849858893996 1107 56-57 0.6119300926332656 1107 58-59 0.32413576940811595 1107 60-61 0.44744923115488433 1107 62-63 0.810754129303433 1107 64-65 0.9816255588955975 1107 66-67 0.6604726636630573 1107 68-69 0.8302029000309368 1107 70-71 0.4450029732958427 1107 72-73 0.5543312560410136 1107 74-75 0.5088718636155711 1107 76-77 0.5864391078661484 1107 78-79 0.5601280889373861 1107 80-81 0.4951277985559486 1107 82-83 0.5420820473792247 1107 84-85 0.930592256221427 1107 86-87 1.1363164604625418 1107 88-89 0.9047215738601935 1107 90-91 1.067834497596909 1107 92-93 0.8369144628610243 1107 94-95 0.8077429952976445 1107 96-97 0.8849960967457804 1107 98-99 0.9690931210208582 1107 100-101 1.6713969839797365 1108 1 0.12785567726425384 1108 2 0.21341924740866247 1108 3 0.4503413498276032 1108 4 0.37734589258339213 1108 5 0.44235189482503756 1108 6 0.1241750104633752 1108 7 0.3327953685897853 1108 8 0.19983703637959138 1108 9 -0.22319903446930311 1108 10-11 0.09856503400332173 1108 12-13 0.16882413085952663 1108 14-15 0.7768577154508307 1108 16-17 0.48049434454530626 1108 18-19 0.5394830088725939 1108 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100-101 -1.4249606545530646 2314 1 -0.11382864655103475 2314 2 -0.5472675315911033 2314 3 -0.13760806664567582 2314 4 0.08465763374641 2314 5 0.11038068438545423 2314 6 0.7518782358358536 2314 7 0.3574985603296028 2314 8 0.03607959515267112 2314 9 0.646709821011143 2314 10-11 0.39697925389367583 2314 12-13 -0.3518767738591393 2314 14-15 -0.30629904639627625 2314 16-17 -0.3635341759538022 2314 18-19 -0.06372722891033789 2314 20-21 -0.2979319882929943 2314 22-23 -0.3193779567198689 2314 24-25 -0.24157995037059976 2314 26-27 0.4282997206447092 2314 28-29 0.3248376864837752 2314 30-31 -0.31391457887513496 2314 32-33 -0.007969714174905107 2314 34-35 -0.1104647733589772 2314 36-37 0.4676665526431165 2314 38-39 0.4157953639246088 2314 40-41 1.0966528732605383 2314 42-43 1.067728531291074 2314 44-45 0.9147571101566996 2314 46-47 1.0234117489793348 2314 48-49 0.63871218386263 2314 50-51 0.15089737286274385 2314 52-53 0.470547756190399 2314 54-55 0.5002973417865277 2314 56-57 0.8065596575702472 2314 58-59 0.31333309292408984 2314 60-61 0.1038864977015237 2314 62-63 -0.16056637697126064 2314 64-65 1.0698541349308535 2314 66-67 1.0878703472682574 2314 68-69 0.7948859409037468 2314 70-71 0.6501732095036168 2314 72-73 0.352902991724779 2314 74-75 0.30367475507823016 2314 76-77 0.7354461752888248 2314 78-79 0.17401973970459395 2314 80-81 0.6527608837681065 2314 82-83 0.9583075599689153 2314 84-85 1.0600765762171278 2314 86-87 1.2023014657026465 2314 88-89 1.4334884026565788 2314 90-91 1.6741279971803849 2314 92-93 1.108264528698232 2314 94-95 1.3120627222747778 2314 96-97 1.8639953711928818 2314 98-99 1.8458028729892568 2314 100-101 1.551284388918873 2315 1 -0.4403319751552033 2315 2 -0.5020858225438474 2315 3 -0.3054024571291407 2315 4 -0.4319853870982868 2315 5 -0.4711701177535801 2315 6 -0.1249906935540821 2315 7 -0.37212056004582195 2315 8 0.19290596206307242 2315 9 0.004916740142427045 2315 10-11 -0.3204440790758696 2315 12-13 -0.2576936460625703 2315 14-15 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-1.2185099994638016 2315 80-81 -1.5616612487212542 2315 82-83 -1.2272621224495097 2315 84-85 -0.7598241113306194 2315 86-87 0.11060114921241393 2315 88-89 -1.0316567463120947 2315 90-91 -1.593674578395298 2315 92-93 -1.4813349474558635 2315 94-95 -1.5525149269667367 2315 96-97 -1.132130351823598 2315 98-99 -1.432763098854025 2315 100-101 -1.974280382444217 2316 1 -5.2572497293813605 2316 2 -4.95386825225393 2316 3 -6.175201879043829 2316 4 -7.080842354778067 2316 5 -7.671813894577614 2316 6 -9.962404199729082 2316 7 -11.01410181606754 2316 8 -11.210965457749488 2316 9 -12.470317559586046 2316 10-11 -12.302729267829484 2316 12-13 -10.477935390829145 2316 14-15 -10.131744870453563 2316 16-17 -8.415070748444673 2316 18-19 -8.402381379606908 2316 20-21 -10.464476249384088 2316 22-23 -9.516033989402164 2316 24-25 -8.805234674843295 2316 26-27 -8.096487408409018 2316 28-29 -6.023123113130644 2316 30-31 -8.243707330934988 2316 32-33 -5.719337366971494 2316 34-35 -4.757431792706711 2316 36-37 -4.5564779471388555 2316 38-39 -3.396373280357139 2316 40-41 -3.347323624868949 2316 42-43 -3.0202772523723738 2316 44-45 -3.819446832832906 2316 46-47 -4.213339760051905 2316 48-49 -5.1495185200459375 2316 50-51 -4.813436702554391 2316 52-53 -4.024632524271379 2316 54-55 -3.809346687499005 2316 56-57 -4.709942144086817 2316 58-59 -4.666320805310974 2316 60-61 -5.078884152501594 2316 62-63 -4.246414762365209 2316 64-65 -4.716678191616619 2316 66-67 -4.539060409427712 2316 68-69 -4.182656461152664 2316 70-71 -4.004379130355467 2316 72-73 -3.2857327981354487 2316 74-75 -3.425089790146803 2316 76-77 -4.23809126934178 2316 78-79 -4.600681322928811 2316 80-81 -4.271764603367831 2316 82-83 -3.7370173120790113 2316 84-85 -3.8620620021951204 2316 86-87 -4.23154214587386 2316 88-89 -4.84273890449726 2316 90-91 -4.024731335862231 2316 92-93 -3.9226505294826666 2316 94-95 -3.446893923200424 2316 96-97 -5.312957188996236 2316 98-99 -6.039533704091827 2316 100-101 -7.076036535549237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 610.0 3 211.0 4 218.0 5 218.0 6 220.0 7 240.0 8 345.0 9 486.0 10 625.0 11 937.0 12 1382.0 13 1776.0 14 2223.0 15 2891.0 16 3975.0 17 5282.0 18 3857.0 19 2499.0 20 2537.0 21 2521.0 22 2760.0 23 3113.0 24 3389.0 25 3671.0 26 4322.0 27 4857.0 28 5597.0 29 6630.0 30 8020.0 31 10036.0 32 12551.0 33 16191.0 34 21592.0 35 29929.0 36 40695.0 37 50544.0 38 39880.0 39 6157.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.07920203454768 18.918651121313207 14.737589589457345 20.264557254681772 2 14.930832975667736 21.816170887120737 39.17890983525372 24.074086301957806 3 20.458304037393173 27.04172071973936 30.579349910709276 21.920625332158195 4 11.480572988712558 16.50726470190127 39.54038091402198 32.47178139536419 5 11.834077282691952 39.15407675444389 34.35494308127097 14.656902881593195 6 28.68179432069174 36.673831007398746 18.108944200680778 16.535430471228736 7 25.96989485992885 35.31788107908132 21.281367778845805 17.430856282144024 8 25.970337541734505 30.69083607710522 21.02989693104849 22.308929450111787 9 26.263136687606103 17.608941204446822 22.47191011235955 33.65601199558753 10-11 24.471271656065678 25.945006968249878 26.998665794809813 22.58505558087463 12-13 27.465359578553233 24.73011591106826 27.364983664150117 20.43954084622839 14-15 23.551484256089065 25.30383420441827 29.11905414373397 22.0256273957587 16-17 20.245174924466312 29.11720517096204 28.858822169756802 21.77879773481484 18-19 19.54055437241015 27.92234163736318 32.240437158469945 20.296666831756724 20-21 21.580818517320377 25.508849995708694 31.307066132343913 21.60326535462702 22-23 20.414694367973173 25.770929472579272 32.20986578466129 21.604510374786265 24-25 21.588806488724124 25.931953566140848 31.739156987444105 20.740082957690916 26-27 20.776367630771468 25.314972261973733 33.663614741015465 20.24504536623933 28-29 19.60184702317691 27.372315776271233 32.747529490979424 20.278307709572438 30-31 19.772070180997268 26.43421521644422 32.1971497221012 21.59656488045731 32-33 19.788125243429867 26.712095906417304 32.41050032677366 21.08927852337917 34-35 21.434064900299383 26.206029238472787 31.464861349960554 20.895044511267276 36-37 20.91517289863061 25.437238638726523 32.97368481851997 20.6739036441229 38-39 20.527813260190054 26.501896246125817 32.191294761475675 20.778995732208458 40-41 19.9860055383742 27.394323661532066 32.39552837344089 20.224142426652847 42-43 19.91227187138489 27.48996225842172 30.32792431221048 22.269841557982915 44-45 20.02465436038932 26.732818683186515 30.656723511414608 22.585803445009557 46-47 21.304273924587896 27.586855606730946 30.181343516338078 20.927526952343076 48-49 21.059909963430893 27.479636157207548 29.554470804124257 21.905983075237305 50-51 19.799529352744507 28.035196229541203 30.699336935248045 21.465937482466245 52-53 20.765110284531783 28.878276109033663 28.411427477565194 21.945186128869366 54-55 19.697414757631464 27.253134602246288 29.91445233985392 23.13499830026833 56-57 21.235865310352565 27.29769158156698 28.630461617675213 22.835981490405246 58-59 22.07692548884998 26.959896891394358 28.71181903845614 22.25135858129952 60-61 21.04424112801014 27.161062262357415 30.740718736797636 21.05397787283481 62-63 20.616498149251367 27.722613788448626 30.066290301021002 21.594597761279005 64-65 20.13558270363397 28.712970954343152 29.62686702630628 21.5245793157166 66-67 20.592860785145312 27.601377610041304 28.607026339283504 23.19873526552988 68-69 20.0114197260916 27.552399545191253 29.88568722162355 22.5504935070936 70-71 21.268109305200067 27.49594453878308 28.778179885902507 22.457766270114345 72-73 20.819806601430273 26.665665399678822 30.211470888712476 22.30305711017843 74-75 19.822351906284194 28.914191217609016 29.019981284565375 22.243475591541415 76-77 20.156769925526856 27.275623250409335 29.2151930491734 23.352413774890405 78-79 20.30414711557029 27.094312343309273 29.95027158350264 22.65126895761779 80-81 20.975724878657402 27.66000637038333 28.77267798702488 22.591590763934388 82-83 20.413629439907055 27.060679623667184 29.938295544373904 22.58739539205186 84-85 20.206846019031023 27.44959221596216 28.813053049881344 23.53050871512547 86-87 20.211064812904823 27.710714138372044 29.146912001320185 22.931309047402944 88-89 20.03313689929056 27.05690536345799 28.243595815641342 24.66636192161011 90-91 19.810609248724713 26.310725596301964 29.216279419021262 24.66238573595206 92-93 20.883718397074187 27.024493453723856 29.467072222763186 22.62471592643877 94-95 20.038587161929094 26.737536330192558 29.30082637262523 23.923050135253117 96-97 18.66773614831466 26.946646399329268 30.527429398532103 23.858188053823977 98-99 19.714278640223824 27.761849348419947 28.128120692927943 24.395751318428285 100-101 19.246475176860127 26.702825470499103 29.1095426890662 24.94115666357457 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 160.0 1 134.5 2 159.0 3 201.5 4 186.0 5 203.5 6 306.0 7 589.5 8 958.5 9 1109.0 10 932.5 11 714.0 12 705.5 13 751.0 14 769.0 15 779.5 16 781.0 17 790.0 18 782.0 19 815.0 20 885.5 21 990.5 22 1133.5 23 1278.5 24 1435.5 25 1637.5 26 1942.0 27 2305.5 28 2710.0 29 3198.5 30 3741.0 31 4372.0 32 5250.5 33 6032.5 34 6624.5 35 7114.5 36 7980.0 37 8721.5 38 8719.5 39 8651.0 40 8622.5 41 8761.5 42 8827.5 43 9011.0 44 9106.5 45 9324.0 46 9881.5 47 13139.5 48 21223.0 49 20735.5 50 12549.5 51 8911.5 52 7644.0 53 7513.5 54 7375.0 55 6570.5 56 5849.0 57 5235.0 58 4705.5 59 4170.5 60 3611.5 61 3207.0 62 2741.5 63 2047.0 64 1401.5 65 1053.0 66 805.0 67 637.5 68 451.0 69 285.5 70 221.5 71 171.0 72 148.5 73 148.5 74 113.0 75 92.5 76 73.0 77 47.0 78 32.5 79 22.0 80 16.0 81 12.0 82 7.0 83 5.5 84 5.0 85 2.0 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07227985174379267 2 0.03267445352801587 3 0.015182069316047776 4 0.028383868721306712 5 0.033334543498278815 6 0.03300449851314734 7 0.08251124628286835 8 0.06006818729392817 9 0.06963949186274089 10-11 0.06171841221958553 12-13 0.04208073560426286 14-15 0.036304948364462075 16-17 0.04752647785893217 18-19 0.040925578156302704 20-21 0.016832294241705147 22-23 0.014191934360653356 24-25 0.020957856555848562 26-27 0.021617946526111508 28-29 0.004950674776972101 30-31 3.300449851314734E-4 32-33 0.008911214598549783 34-35 0.010561439524207149 36-37 0.002640359881051787 38-39 0.007260989672892416 40-41 0.003960539821577681 42-43 0.0024753373884860504 44-45 0.0 46-47 1.650224925657367E-4 48-49 3.300449851314734E-4 50-51 6.600899702629468E-4 52-53 0.0 54-55 0.0 56-57 0.003630494836446208 58-59 0.0024753373884860504 60-61 0.004290584806709154 62-63 0.001155157447960157 64-65 1.650224925657367E-4 66-67 1.650224925657367E-4 68-69 0.0014852024330916304 70-71 8.251124628286836E-4 72-73 0.0130367769126932 74-75 0.01006637204650994 76-77 0.02013274409301988 78-79 0.014356956853219093 80-81 0.008086102135721099 82-83 0.004125562314143417 84-85 0.0033004498513147344 86-87 4.950674776972101E-4 88-89 0.001155157447960157 90-91 0.005775787239800784 92-93 0.01006637204650994 94-95 0.012706731927561728 96-97 0.012706731927561728 98-99 0.02359821643690035 100-101 0.021782969018677246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302989.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.85462859560517 #Duplication Level Percentage of deduplicated Percentage of total 1 81.09311558469776 42.86146505889681 2 10.41540234788593 11.0100444554261 3 3.2917979726309103 5.219602777655185 4 1.4707039921291336 3.109340531122368 5 0.7894162859070386 2.0862152299469297 6 0.5549575001138212 1.759924353291692 7 0.3511916922590103 1.299347452214844 8 0.3040621917014153 1.2856875369875205 9 0.22207549607599322 1.0563946078753257 >10 1.370828815206932 13.313129282148278 >50 0.08048552782572468 2.891080491258108 >100 0.04024276391286234 3.856214155657872 >500 0.00943189779207711 3.4042231833494427 >1k 0.0062879318613847405 6.847330884169538 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3390 1.118852499595695 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3068 1.0125780143833605 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2724 0.8990425394981336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2424 0.8000290439586916 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2012 0.6640505100845245 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1921 0.6340164164375603 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1413 0.46635356399077194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1346 0.4442405499869633 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1253 0.4135463663697362 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1052 0.34720732435831003 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 953 0.3145328708302942 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 867 0.28614900210898747 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 776 0.25611490846202334 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 726 0.23961265920544972 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 723 0.23862252425005526 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 722 0.2382924792649238 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 711 0.2346619844284776 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 708 0.23367184947308317 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 681 0.2247606348745334 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 622 0.20528798075177646 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 597 0.19703685612348965 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 562 0.18548528164388806 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 542 0.1788843819412586 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 531 0.17525388710481238 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 522 0.17228348223862913 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 488 0.16106195274415905 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 428 0.14125925363627062 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 395 0.13036776912693201 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.12442695939456547 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 359 0.11848614966219896 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 339 0.1118852499595695 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 322 0.10627448521233443 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 315 0.10396417031641413 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 314 0.10363412533128265 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 304 0.10033367547996792 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.650224925657367E-4 0.0 22-23 0.0 0.0 0.0 4.950674776972101E-4 0.0 24-25 0.0 0.0 0.0 9.901349553944202E-4 0.0 26-27 0.0 0.0 0.0 0.0019802699107888404 0.0 28-29 0.0 0.0 0.0 0.005610764747235048 0.0 30-31 0.0 0.0 0.0 0.011881619464733043 0.0 32-33 0.0 0.0 0.0 0.018977586645059722 0.0 34-35 0.0 0.0 0.0 0.024753373884860506 0.0 36-37 0.0 0.0 0.0 0.033169521005713076 0.0 38-39 0.0 0.0 0.0 0.042245758096828595 0.0 40-41 0.0 0.0 0.0 0.055117512516956055 0.0 42-43 0.0 0.0 0.0 0.08069599886464525 0.0 44-45 0.0 0.0 0.0 0.1186511721547647 0.0 46-47 0.0 0.0 0.0 0.1613919977292905 0.0 48-49 0.0 0.0 0.0 0.24885391878913096 0.0 50-51 0.0 0.0 0.0 0.3285597826983818 0.0 52-53 0.0 0.0 0.0 0.4021598143827003 0.0 54-55 0.0 0.0 0.0 0.5351679433906842 0.0 56-57 0.0 0.0 0.0 0.6770872869972178 0.0 58-59 0.0 0.0 0.0 0.8497008142209783 0.0 60-61 0.0 0.0 0.0 1.0483878952701253 0.0 62-63 0.0 0.0 0.0 1.340147662126348 0.0 64-65 0.0 0.0 0.0 1.541310080563981 0.0 66-67 0.0 0.0 0.0 1.8020456188178449 0.0 68-69 0.0 0.0 0.0 2.1452924033545773 0.0 70-71 0.0 0.0 0.0 2.3781391403648318 0.0 72-73 0.0 0.0 0.0 2.7057087881078195 0.0 74-75 0.0 0.0 0.0 3.1091887824310454 0.0 76-77 0.0 0.0 0.0 3.4039189541534514 0.0 78-79 0.0 0.0 0.0 3.6630042674816576 0.0 80-81 0.0 0.0 0.0 4.050147035040876 0.0 82-83 0.0 0.0 0.0 4.480360673159751 0.0 84-85 0.0 0.0 0.0 5.041932215360953 0.0 86-87 0.0 0.0 0.0 5.884537062401606 0.0 88-89 0.0 0.0 0.0 6.738033393951596 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAGAC 40 5.361086E-4 47.53726 3 GTACTAA 45 9.57679E-4 42.255344 1 GTCGGGA 85 3.6066194E-7 39.14833 2 ATATCGT 30 0.0039375396 31.686274 10-11 ATCGTAG 30 0.0039407257 31.681038 12-13 GTATAAA 140 1.6842023E-8 30.559666 1 GGTCGGG 110 2.7080787E-6 30.250984 1 GGTACCT 65 0.0058197347 29.248863 8 GTACATG 2365 0.0 28.944464 1 CGGGAGT 115 3.8287617E-6 28.935722 4 GTAAGGT 100 4.914881E-5 28.522354 4 ATGGGTA 170 3.588866E-9 27.963095 5 GGTAAGG 85 6.3669315E-4 27.963095 3 GGGTACC 85 6.3669315E-4 27.963095 7 TACATGG 2385 0.0 27.904472 2 GTCCTAT 70 0.0083499765 27.164148 1 ATATGGC 70 0.0083499765 27.164148 3 CTTTGCG 70 0.0083499765 27.164148 2 GTATAGG 70 0.0083499765 27.164148 1 TAGGATA 70 0.0083499765 27.164148 7 >>END_MODULE