##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936556_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302989 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00080200931387 31.0 31.0 34.0 30.0 34.0 2 32.13504120611639 33.0 31.0 34.0 30.0 34.0 3 32.18380865311942 33.0 31.0 34.0 30.0 34.0 4 35.88073494417289 37.0 35.0 37.0 35.0 37.0 5 35.75914637165046 37.0 35.0 37.0 35.0 37.0 6 35.8939334431283 37.0 35.0 37.0 35.0 37.0 7 35.842469528596745 37.0 35.0 37.0 35.0 37.0 8 35.83577291584843 37.0 35.0 37.0 35.0 37.0 9 37.46113225232599 39.0 37.0 39.0 35.0 39.0 10-11 37.344746838994155 39.0 37.0 39.0 34.0 39.0 12-13 37.261940202449594 39.0 37.0 39.0 34.0 39.0 14-15 38.26146328744608 40.0 38.0 41.0 33.0 41.0 16-17 38.22877398189373 40.0 37.5 41.0 33.0 41.0 18-19 38.46816716118407 40.0 38.0 41.0 34.0 41.0 20-21 38.51739502094135 40.0 38.0 41.0 34.0 41.0 22-23 38.43409001646924 40.0 38.0 41.0 34.0 41.0 24-25 38.25494654921465 40.0 37.5 41.0 33.5 41.0 26-27 37.99419120826168 40.0 37.0 41.0 33.0 41.0 28-29 37.89729165085201 40.0 37.0 41.0 33.0 41.0 30-31 37.8094006713115 40.0 37.0 41.0 33.0 41.0 32-33 37.5399932010733 39.5 36.0 41.0 33.0 41.0 34-35 37.25398116763315 39.0 35.0 41.0 33.0 41.0 36-37 36.96478089963662 39.0 35.0 41.0 31.5 41.0 38-39 36.731246018832366 39.0 35.0 41.0 31.0 41.0 40-41 36.53068923294245 39.0 35.0 41.0 31.0 41.0 42-43 36.15949093861494 39.0 35.0 40.0 30.0 41.0 44-45 35.656723511414604 39.0 35.0 40.0 29.5 41.0 46-47 35.00162217110192 38.0 35.0 40.0 25.5 41.0 48-49 34.34242992319854 38.0 34.0 40.0 22.5 41.0 50-51 33.946227420797456 38.0 34.0 40.0 20.0 41.0 52-53 33.60219842964596 38.0 33.0 40.0 17.5 41.0 54-55 33.43750763229028 38.0 33.0 40.0 16.0 41.0 56-57 33.062144170250406 37.5 33.0 40.0 12.0 41.0 58-59 32.68851674483232 37.0 32.5 40.0 8.0 41.0 60-61 32.182500024753374 36.0 31.0 39.0 7.0 41.0 62-63 31.904339761509494 36.0 31.0 39.0 3.5 40.0 64-65 31.487662918455786 35.5 31.0 39.0 2.0 40.0 66-67 31.022342395268474 35.0 30.5 38.0 2.0 40.0 68-69 30.609127724108795 35.0 30.0 38.0 2.0 40.0 70-71 30.194000442260283 34.5 29.5 37.0 2.0 39.5 72-73 29.7125704233487 34.0 29.0 36.5 2.0 39.0 74-75 29.21421239714973 34.0 29.0 36.0 2.0 39.0 76-77 27.779361626989758 32.5 26.5 34.5 2.0 36.5 78-79 28.48582621811353 34.0 28.0 35.0 2.0 37.0 80-81 28.18102637389476 34.0 27.5 35.0 2.0 37.0 82-83 27.862280478829263 33.5 26.5 35.0 2.0 36.5 84-85 27.49681176544363 33.0 26.0 35.0 2.0 36.0 86-87 27.179235879850424 33.0 26.0 35.0 2.0 35.5 88-89 26.700201987530903 33.0 25.0 35.0 2.0 35.0 90-91 26.19363574255171 33.0 24.5 35.0 2.0 35.0 92-93 25.539004716342838 32.0 21.5 34.0 2.0 35.0 94-95 25.011132417348485 32.0 19.5 34.0 2.0 35.0 96-97 24.321198129305024 31.5 11.5 34.0 2.0 35.0 98-99 23.577659585001435 31.0 2.0 34.0 2.0 35.0 100-101 20.6898468261224 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14239052377793016 1101 2 0.1431202781657035 1101 3 0.08744878183090776 1101 4 0.20008930475396625 1101 5 0.1288749130828677 1101 6 0.03965424335361689 1101 7 -0.006278590833147746 1101 8 0.10770158619212111 1101 9 -0.0051611024715114695 1101 10-11 0.18038476333565967 1101 12-13 0.38217052098065807 1101 14-15 0.3036090082514562 1101 16-17 0.18773262716818806 1101 18-19 0.15453002350574963 1101 20-21 0.01819784519960166 1101 22-23 0.1615465095824007 1101 24-25 0.11601948425162334 1101 26-27 -0.009701567981444725 1101 28-29 0.004755658652086936 1101 30-31 0.06380132292794372 1101 32-33 0.14773506412089432 1101 34-35 0.045971417778943646 1101 36-37 0.21845417260299627 1101 38-39 0.3349227478694772 1101 40-41 -0.09480805867202946 1101 42-43 0.09647870636247546 1101 44-45 0.32717861312097796 1101 46-47 0.17059633799959784 1101 48-49 0.5984184550945173 1101 50-51 0.49492631612015003 1101 52-53 0.2611231349849348 1101 54-55 0.42987211976889483 1101 56-57 0.46816970010399217 1101 58-59 0.3063038759958374 1101 60-61 0.5783431893385398 1101 62-63 0.539800692216204 1101 64-65 0.8030587327936836 1101 66-67 0.5150322753304835 1101 68-69 0.5784002587008175 1101 70-71 0.8179883337485236 1101 72-73 0.3500245644381259 1101 74-75 0.1607711537880725 1101 76-77 0.06562453438814941 1101 78-79 0.32821836719244857 1101 80-81 0.1731359482397714 1101 82-83 0.21962894086086848 1101 84-85 0.3227131723560639 1101 86-87 0.4652037537337357 1101 88-89 0.5278135024278789 1101 90-91 0.7667121158865378 1101 92-93 0.562453728750107 1101 94-95 0.5556685375804449 1101 96-97 0.5960143525142385 1101 98-99 0.7497485448186083 1101 100-101 1.1995335290218065 1102 1 0.1819355649285761 1102 2 0.12139408434784116 1102 3 0.17025907716492128 1102 4 0.19719247891158886 1102 5 0.15771592075908814 1102 6 -0.029701156756416935 1102 7 0.15905755698731383 1102 8 0.26766700393012854 1102 9 0.3316080010469662 1102 10-11 0.3598981415061644 1102 12-13 0.2641379672452828 1102 14-15 0.37198195773665077 1102 16-17 0.3701821292060714 1102 18-19 0.21418816811499397 1102 20-21 0.15534233842350176 1102 22-23 0.1684367632979331 1102 24-25 0.2323959069336965 1102 26-27 0.3309243559899784 1102 28-29 0.24062240466334117 1102 30-31 0.37182485683091215 1102 32-33 0.4850749246690427 1102 34-35 0.5840549466667326 1102 36-37 0.40162226709791327 1102 38-39 0.6136299551272657 1102 40-41 0.6385754264265344 1102 42-43 0.8548594780259791 1102 44-45 0.830391694497834 1102 46-47 0.9214768008929042 1102 48-49 1.0739320428544872 1102 50-51 1.1980765206570183 1102 52-53 1.020774542789887 1102 54-55 0.7578702783757265 1102 56-57 0.8285718873197823 1102 58-59 0.7233195465511315 1102 60-61 0.48652616094291545 1102 62-63 0.29432090075772166 1102 64-65 0.3345907195146971 1102 66-67 0.3187086991353567 1102 68-69 0.41038623117723105 1102 70-71 0.5463173442991085 1102 72-73 0.9103245767889341 1102 74-75 0.37550866169446095 1102 76-77 0.6241074809166385 1102 78-79 0.9004705931448669 1102 80-81 1.2686563476574122 1102 82-83 1.3945631634523679 1102 84-85 1.099408695001081 1102 86-87 1.051217773771988 1102 88-89 1.1355350029387559 1102 90-91 0.6559665387856803 1102 92-93 0.5783357448510706 1102 94-95 0.5099705485165202 1102 96-97 1.1411883679115746 1102 98-99 1.1145128128086803 1102 100-101 1.70308906461646 1103 1 0.18792373060905376 1103 2 0.2918792914484314 1103 3 0.22220779511363986 1103 4 0.1915238398583341 1103 5 0.2517553684908407 1103 6 0.06181363614299329 1103 7 0.12445006191827446 1103 8 -0.02542175346632547 1103 9 0.20374212333493347 1103 10-11 0.1787206267132646 1103 12-13 0.2762957581723029 1103 14-15 0.25035473880174663 1103 16-17 0.11363775050784852 1103 18-19 0.30919605766134595 1103 20-21 0.14858494197379457 1103 22-23 0.03087857789360271 1103 24-25 0.09087716926111256 1103 26-27 0.1824312593430335 1103 28-29 0.23653364726688864 1103 30-31 0.16340473848771353 1103 32-33 0.3741695992252616 1103 34-35 0.1945539604734421 1103 36-37 0.3771429771570851 1103 38-39 0.41869504388466083 1103 40-41 0.6439205940793968 1103 42-43 0.7645261446926455 1103 44-45 0.6817270002177551 1103 46-47 0.726776603654244 1103 48-49 0.8531490053791444 1103 50-51 0.9890686311106691 1103 52-53 1.26295994712914 1103 54-55 1.2011187990857835 1103 56-57 1.1929742541836887 1103 58-59 1.0949566274189877 1103 60-61 1.2739048591677644 1103 62-63 1.1837134056886924 1103 64-65 1.2777228694160776 1103 66-67 0.9908349318769716 1103 68-69 1.1315558564237769 1103 70-71 1.0098479162912177 1103 72-73 0.8524192513451432 1103 74-75 0.6851753283611295 1103 76-77 1.0270818398909967 1103 78-79 0.9906244392987134 1103 80-81 1.0126938230025786 1103 82-83 1.0750463981663714 1103 84-85 1.552481673304829 1103 86-87 1.4387483457640968 1103 88-89 1.6180734644772166 1103 90-91 1.392112495393178 1103 92-93 1.5808446205907138 1103 94-95 1.4191125603508397 1103 96-97 1.4708530621916545 1103 98-99 1.7569743512702196 1103 100-101 2.4474007016973296 1104 1 0.14809150102847823 1104 2 0.18008238195616144 1104 3 0.14207092977147084 1104 4 0.160278806745751 1104 5 0.10128737732086535 1104 6 0.16326617477433558 1104 7 0.14726242394917932 1104 8 0.06350539885155371 1104 9 -0.0633395543692572 1104 10-11 0.035460141062053196 1104 12-13 -0.03663139414558003 1104 14-15 0.1069441427752551 1104 16-17 -0.03543068216323064 1104 18-19 0.21614749253993182 1104 20-21 -0.04158282844563388 1104 22-23 -0.07283444638896697 1104 24-25 0.06825023769378191 1104 26-27 0.10471382004943308 1104 28-29 0.2671451262955884 1104 30-31 0.1173804559270053 1104 32-33 0.10792456611267909 1104 34-35 0.29186742625710593 1104 36-37 0.34661096832706306 1104 38-39 0.3582712028250583 1104 40-41 0.35122743734650186 1104 42-43 0.591062568533701 1104 44-45 0.5405945498866274 1104 46-47 0.36127329239597117 1104 48-49 0.4335529788890753 1104 50-51 0.5493180792342898 1104 52-53 0.6065537661136915 1104 54-55 0.40788921851183346 1104 56-57 0.4936188457951687 1104 58-59 0.5757954795161169 1104 60-61 0.820088082125821 1104 62-63 0.76155446529134 1104 64-65 0.5186235316677319 1104 66-67 0.6403448656518087 1104 68-69 0.769288748256006 1104 70-71 0.7895609405737787 1104 72-73 0.7715848142590538 1104 74-75 0.5587316197518568 1104 76-77 0.34842400324640366 1104 78-79 0.7631807306894416 1104 80-81 0.9037909532454051 1104 82-83 0.983666707217143 1104 84-85 1.4508657792650972 1104 86-87 1.2922538645278934 1104 88-89 1.1743361576339488 1104 90-91 1.443869669786114 1104 92-93 1.5358379980741539 1104 94-95 1.4966732667526088 1104 96-97 1.5270120025890037 1104 98-99 1.6375218126388766 1104 100-101 2.345603792205054 1105 1 -0.06584550016017943 1105 2 -0.07775532393618434 1105 3 -0.04448451257866992 1105 4 -0.03320692937243308 1105 5 0.06698613772160655 1105 6 0.05406363614299181 1105 7 0.1399500619182774 1105 8 0.12325132345675627 1105 9 -0.25764249204967626 1105 10-11 -0.005856296363653257 1105 12-13 -0.060319626443082086 1105 14-15 0.1025662772632785 1105 16-17 -0.07582378795368783 1105 18-19 0.015994134584424557 1105 20-21 -0.004328519564673172 1105 22-23 -0.07465988364486975 1105 24-25 0.11130986156880596 1105 26-27 -0.04937643296465666 1105 28-29 0.04091826265150189 1105 30-31 0.009491276949255223 1105 32-33 0.1656407530714148 1105 34-35 0.3839193450888274 1105 36-37 0.26071990023400105 1105 38-39 -0.03439149457687307 1105 40-41 0.1367571325409358 1105 42-43 0.36167037546186975 1105 44-45 0.35365969252544716 1105 46-47 0.3035650651927071 1105 48-49 0.8092932361483705 1105 50-51 0.7328090157260547 1105 52-53 0.8974695625137556 1105 54-55 0.7341091837011717 1105 56-57 0.3444742541836874 1105 58-59 0.592648935111292 1105 60-61 0.7111933207062293 1105 62-63 0.7884345595348421 1105 64-65 0.5578094078776168 1105 66-67 0.4396714703385136 1105 68-69 0.8325077795007019 1105 70-71 0.7709536855219881 1105 72-73 0.6978615590374524 1105 74-75 0.7440984052842055 1105 76-77 0.7390914552756094 1105 78-79 0.8415763623756369 1105 80-81 1.2151553614641202 1105 82-83 1.2458637058586781 1105 84-85 1.380597057920216 1105 86-87 1.3191233457640976 1105 88-89 1.134333079861829 1105 90-91 1.07170864923933 1105 92-93 1.3170561590522532 1105 94-95 1.193679868043148 1105 96-97 1.2825838314224214 1105 98-99 1.305618582039454 1105 100-101 1.6791122401588687 1106 1 -0.007576269390948909 1106 2 0.10887929144843156 1106 3 0.12320779511363611 1106 4 0.11352383985833114 1106 5 0.15525536849084176 1106 6 -0.08718636385700762 1106 7 0.012450061918279687 1106 8 0.05757824653367294 1106 9 0.14274212333493352 1106 10-11 0.21847062671326256 1106 12-13 0.10179575817230813 1106 14-15 0.1678547388017506 1106 16-17 -0.1736122494921517 1106 18-19 0.24969605766134606 1106 20-21 0.15283494197379355 1106 22-23 -0.08562142210639934 1106 24-25 -0.11787283073888233 1106 26-27 -0.024568740656967236 1106 28-29 -0.3032163527331164 1106 30-31 -0.08459526151229113 1106 32-33 -0.12008040077473936 1106 34-35 0.12055396047344402 1106 36-37 -0.2923570228429213 1106 38-39 -0.18530495611533837 1106 40-41 0.17142059407940025 1106 42-43 -0.0022238553073563594 1106 44-45 0.001977000217756597 1106 46-47 0.08452660365425402 1106 48-49 -0.017100994620854237 1106 50-51 -0.32743136888932867 1106 52-53 -0.5710400528708561 1106 54-55 -0.46863120091420996 1106 56-57 -0.47677574581631177 1106 58-59 -0.7975433725810142 1106 60-61 -0.6790951408322385 1106 62-63 -0.4960365943113132 1106 64-65 -0.6162771305839243 1106 66-67 -0.5119150681230273 1106 68-69 -0.6536941435762209 1106 70-71 -0.46265208370877886 1106 72-73 -0.4023307486548511 1106 74-75 -0.29907467163887347 1106 76-77 -0.5919181601090067 1106 78-79 -0.6371255607012856 1106 80-81 -0.0883061769974205 1106 82-83 0.11129639816637038 1106 84-85 0.28348167330483065 1106 86-87 0.538498345764097 1106 88-89 0.3908234644772186 1106 90-91 0.22411249539317524 1106 92-93 0.5210946205907128 1106 94-95 -0.13588743964916006 1106 96-97 0.262103062191656 1106 98-99 0.5942243512702206 1106 100-101 1.304400701697329 1107 1 -0.21887023924019644 1107 2 -0.2571257336771957 1107 3 -0.2550082852883726 1107 4 -0.14305907471452883 1107 5 -0.27259639030312854 1107 6 -0.3597240522992209 1107 7 -0.2121228024033286 1107 8 -0.12405491929546741 1107 9 -0.15858953495651917 1107 10-11 -0.187364800422408 1107 12-13 -0.4169102719784519 1107 14-15 -0.10941913054499253 1107 16-17 -0.1296989329092355 1107 18-19 -0.24348233429845578 1107 20-21 -0.2864301334030941 1107 22-23 -0.04528222612650268 1107 24-25 -0.2973137855127561 1107 26-27 -0.13941798688811957 1107 28-29 -0.10964223213009916 1107 30-31 -0.5741178745776097 1107 32-33 -0.5798354259003631 1107 34-35 -0.48263699430042806 1107 36-37 -0.5697062690740751 1107 38-39 -0.50354616214549 1107 40-41 -0.31949649134774205 1107 42-43 -0.3352577749053438 1107 44-45 -0.034549381691796555 1107 46-47 -0.17603244157188414 1107 48-49 -0.16464496447010646 1107 50-51 -0.5176198111003885 1107 52-53 -0.32053754030804527 1107 54-55 -0.5651575828237654 1107 56-57 -0.5050860473238501 1107 58-59 -0.5215760358975992 1107 60-61 -0.3861805679679158 1107 62-63 -0.316809207376636 1107 64-65 -0.4673826582221139 1107 66-67 -0.421104766615489 1107 68-69 -0.44774565111390885 1107 70-71 -0.4276897721509947 1107 72-73 -0.6256913014186729 1107 74-75 -0.5445080887242959 1107 76-77 -0.6136518284507098 1107 78-79 -0.7533755607012864 1107 80-81 -0.13172326242455767 1107 82-83 -0.38492847620548787 1107 84-85 0.024562075314879195 1107 86-87 0.2945473407389727 1107 88-89 -0.4092079425579591 1107 90-91 -0.30779705234551713 1107 92-93 -0.2083714598112998 1107 94-95 0.05833366587847877 1107 96-97 -0.04035296795909815 1107 98-99 0.13036128594358942 1107 100-101 1.0805062293355192 1108 1 0.0682256174015059 1108 2 0.2174641971088107 1108 3 0.08317634857275635 1108 4 0.01943578954386993 1108 5 -0.12677293339595508 1108 6 0.028316780797077 1108 7 0.2621984895912348 1108 8 -0.12132112453550548 1108 9 -0.07150315968392817 1108 10-11 0.02846276507804646 1108 12-13 0.26769198458739396 1108 14-15 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-2.6839355199744155 2316 100-101 -3.5708568090322856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 7.0 6 21.0 7 68.0 8 127.0 9 265.0 10 450.0 11 835.0 12 1286.0 13 1798.0 14 2199.0 15 3170.0 16 4481.0 17 6315.0 18 5061.0 19 2892.0 20 2308.0 21 2332.0 22 2367.0 23 2614.0 24 3095.0 25 3487.0 26 4036.0 27 4741.0 28 5787.0 29 6972.0 30 8599.0 31 10603.0 32 13171.0 33 17172.0 34 23000.0 35 31626.0 36 43869.0 37 54147.0 38 32170.0 39 1911.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.39233110405429 19.6403224422159 15.237771425176108 18.729575028553697 2 16.048404666178133 23.81843628897559 37.71827322361081 22.414885821235465 3 21.9675540078887 27.444424273430922 30.231874969055834 20.356146749624543 4 12.132783698418095 18.191749535461682 39.02253877203463 30.652927994085594 5 12.004805486704049 40.24760138223753 33.26578368048794 14.481809450570488 6 28.799725402572367 38.755202334078135 16.43525012459198 16.009822138757514 7 26.531326219763752 36.48680315126952 20.24594952291997 16.735921106046757 8 26.85245999029668 31.412691549858245 19.460772503292198 22.274075956552878 9 27.112535438580277 18.658763189422718 20.67995867836786 33.548742693629144 10-11 25.097775826845197 27.01632732541445 25.972230014951037 21.913666832789307 12-13 29.022510978248135 25.395274691363117 26.149931267337877 19.432283063050875 14-15 23.902887403317287 26.464865828241006 28.773699331822826 20.85854743661888 16-17 20.198093000075907 29.69579753720432 28.69064553498642 21.41546392773335 18-19 19.645294215691614 28.500726937275463 32.57735157195029 19.276627275082635 20-21 21.973735020083236 25.272204601487182 32.31008386443072 20.44397651399886 22-23 20.790523748386907 25.977840779698276 32.328071316120386 20.903564155794434 24-25 22.43926347161118 26.05111076639746 32.49210367373073 19.01752208826063 26-27 21.454115185250586 25.287352528124657 34.2806408972165 18.977891389408263 28-29 19.525176128102768 28.030154253396727 33.42795044483795 19.01671917366256 30-31 20.32552927799699 26.404408160840443 32.437079906991634 20.832982654170937 32-33 20.58738101210673 27.106630315672735 32.309256300912296 19.99673237130824 34-35 22.013043593061017 25.751194782562774 32.49131967364613 19.74444195073007 36-37 21.92017505643788 25.41618809985874 33.24587112360886 19.417765720094526 38-39 20.790564580541677 26.56855432264197 33.283386031037736 19.357495065778615 40-41 20.203935469391528 27.39415422392966 32.46267178898174 19.93923851769707 42-43 20.31097608269812 28.081747565445415 30.53503266701102 21.072243684845443 44-45 21.33721908860776 26.29782795961543 30.663856865146027 21.70109608663078 46-47 22.3414051335197 28.134189690054757 29.741343745152466 19.783061431273083 48-49 22.607128653397737 27.910135203151277 28.975687196048693 20.507048947402296 50-51 20.697616085072397 28.452188033228932 30.56992168032503 20.280274201373647 52-53 21.71022992621832 29.753274464212335 27.707157202336063 20.829338407233276 54-55 20.13739908774663 28.354280395793864 28.853973450918524 22.654347065540982 56-57 22.329992936314603 27.910469438007908 28.327028829078614 21.432508796598867 58-59 23.702323339693525 27.16026356271829 27.640743944065548 21.496669153522642 60-61 21.301496813762302 28.166638333550647 30.45023246909077 20.081632383596283 62-63 21.202208879749403 28.16915820292515 29.880115791245682 20.748517126079765 64-65 20.791191887716703 29.80594029338368 28.920474800955944 20.48239301794367 66-67 21.428618578477483 28.014753205604237 28.085713814215225 22.470914401703055 68-69 20.987760261528077 28.600590451452778 28.380615105853852 22.03103418116529 70-71 22.520289515460956 28.47578624966583 28.082372627389113 20.9215516074841 72-73 21.84782285825558 27.389443181105584 29.434896976457892 21.327836984180944 74-75 20.697917732443965 30.362853851815423 27.77759441824237 21.161633997498242 76-77 21.226228274573742 28.27622398394645 28.022251853220943 22.475295888258863 78-79 21.347292888251495 28.042169122076245 28.445673753715294 22.164864235956966 80-81 22.046178475351123 28.487729200706376 27.84340908715816 21.62268323678434 82-83 21.69664180520919 28.005862440604 28.435972806990033 21.861522947196775 84-85 20.81954305126601 28.581497884618877 28.129864427427485 22.469094636687625 86-87 20.859368553085442 29.010579128913534 27.177303559934646 22.95274875806638 88-89 20.413385501791225 28.919485579383554 26.631337394548726 24.035791524276494 90-91 21.129916079320974 27.140241476264297 27.65205360489094 24.077788839523784 92-93 21.9724694164236 28.13078265233188 27.48188755676388 22.414860374480643 94-95 21.40779551787436 27.964753113320917 27.377415052229836 23.250036316574885 96-97 19.86183530530633 28.206368652729115 28.803904539555585 23.127891502408964 98-99 21.004322685678996 29.20669306289854 26.032540675844807 23.756443575577652 100-101 19.94322929223209 28.540078006056863 26.503730221897836 25.012962479813204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 478.0 1 332.5 2 242.5 3 301.0 4 272.0 5 254.5 6 359.0 7 567.0 8 875.5 9 1110.5 10 1010.0 11 842.0 12 821.5 13 841.5 14 849.0 15 884.0 16 945.0 17 959.5 18 993.5 19 1030.5 20 1045.5 21 1089.5 22 1191.0 23 1303.5 24 1411.5 25 1631.0 26 1945.5 27 2277.0 28 2636.0 29 3119.0 30 3718.0 31 4381.0 32 5208.5 33 5796.0 34 6214.0 35 6867.0 36 7896.0 37 8435.0 38 8689.0 39 8749.0 40 8390.5 41 8522.0 42 8723.5 43 9005.0 44 8999.0 45 9057.0 46 9404.5 47 13294.0 48 21818.5 49 20215.5 50 11775.0 51 8636.0 52 7591.0 53 7466.5 54 7089.5 55 6404.5 56 5800.0 57 5246.0 58 4970.5 59 4616.0 60 3981.5 61 3338.0 62 2741.5 63 2121.5 64 1469.0 65 1110.0 66 856.0 67 698.0 68 532.0 69 328.0 70 231.5 71 208.5 72 187.5 73 162.5 74 149.0 75 132.5 76 95.5 77 64.0 78 51.0 79 42.5 80 39.5 81 32.5 82 23.5 83 16.0 84 10.0 85 6.5 86 3.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016832294241705147 2 0.014191934360653356 3 0.007921079643155362 4 0.0 5 6.600899702629468E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001155157447960157 14-15 1.650224925657367E-4 16-17 0.0 18-19 0.001815247418223104 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0024753373884860504 28-29 0.004455607299274892 30-31 0.001815247418223104 32-33 0.005610764747235049 34-35 0.0016502249256573672 36-37 3.300449851314734E-4 38-39 9.901349553944202E-4 40-41 3.300449851314734E-4 42-43 0.0024753373884860504 44-45 6.600899702629468E-4 46-47 0.0 48-49 1.650224925657367E-4 50-51 0.0 52-53 1.650224925657367E-4 54-55 9.901349553944202E-4 56-57 0.010231394539075676 58-59 0.020792834063282827 60-61 0.015017046823482042 62-63 0.009901349553944202 64-65 0.01353184439039041 66-67 0.0013201799405258936 68-69 1.650224925657367E-4 70-71 0.0 72-73 0.0 74-75 6.600899702629468E-4 76-77 9.901349553944202E-4 78-79 0.006105832224932259 80-81 0.011221529494470098 82-83 0.014356956853219093 84-85 0.029208981184135396 86-87 0.011221529494470098 88-89 0.040925578156302704 90-91 0.047361455366366434 92-93 0.02953902616926687 94-95 0.03201436355775292 96-97 0.05346728759129869 98-99 0.05544755750208753 100-101 0.06336863714524289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302989.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.949547814661415 #Duplication Level Percentage of deduplicated Percentage of total 1 79.12878511518556 38.73318250511844 2 11.347342288084707 11.108945477998638 3 3.6849384537847523 5.411282131128638 4 1.665660179112739 3.2613325032182603 5 0.9465355583127947 2.316624378495469 6 0.6091727053046888 1.7891237079419104 7 0.44783827543706695 1.5345036753719554 8 0.2861738522071996 1.120646452953773 9 0.22940891390320203 1.01065163401929 >10 1.4652560297059172 13.253271464341463 >50 0.1153569841157259 3.868135571038139 >100 0.05869039542729914 5.619924815050204 >500 0.005396817970326357 1.7861315779191818 >1k 0.008769829201780332 7.181510363576699 >5k 6.746022462907947E-4 2.0047337418279443 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6071 2.003703104733175 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3247 1.0716560667218942 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2691 0.888151054988795 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2143 0.7072864031367475 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1667 0.5501849902141662 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1588 0.5241114363887798 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1482 0.48912666796484366 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1413 0.46635356399077194 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1409 0.4650333840502461 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1321 0.4359894253586764 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1295 0.4274082557452581 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1170 0.38615263260382393 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1163 0.3838423177079036 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1159 0.3825221377673777 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 910 0.3003409364696408 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 799 0.26370594312004725 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 774 0.25545481849176044 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 693 0.22872117469611108 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 613 0.2023175758855932 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 584 0.1927462713167805 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 528 0.17426375214941797 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 508 0.1676628524467885 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 493 0.16271217766981638 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 466 0.15380096307126662 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 464 0.15314087310100366 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 417 0.13762875879982442 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 399 0.13168794906745787 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 344 0.11353547488522685 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 340 0.11221529494470096 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 329 0.10858480010825476 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 319 0.10528435025694002 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 310 0.10231394539075675 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 306 0.10099376545023087 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.1006637204650994 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.300449851314734E-4 0.0 16-17 0.0 0.0 0.0 3.300449851314734E-4 0.0 18-19 0.0 0.0 0.0 3.300449851314734E-4 0.0 20-21 0.0 0.0 0.0 4.950674776972101E-4 0.0 22-23 0.0 0.0 0.0 8.251124628286835E-4 0.0 24-25 0.0 0.0 0.0 0.0013201799405258936 0.0 26-27 0.0 0.0 0.0 0.002310314895920314 0.0 28-29 0.0 0.0 0.0 0.005775787239800784 0.0 30-31 0.0 0.0 0.0 0.011881619464733043 0.0 32-33 0.0 0.0 0.0 0.018812564152493987 0.0 34-35 0.0 0.0 0.0 0.024918396377426245 0.0 36-37 0.0 0.0 0.0 0.03399463346854176 0.0 38-39 0.0 0.0 0.0 0.04356593803735449 0.0 40-41 0.0 0.0 0.0 0.056107647472350475 0.0 42-43 0.0 0.0 0.0 0.08284129126799983 0.0 44-45 0.0 0.0 0.0 0.12145655452838222 0.0 46-47 0.0 0.0 0.0 0.1640323576103423 0.0 48-49 0.0 0.0 0.0 0.25050414371478835 0.0 50-51 0.0 0.0 0.0 0.33186023254969654 0.0 52-53 0.0 0.0 0.0 0.4052952417414494 0.0 54-55 0.0 0.0 0.0 0.5376432807791702 0.0 56-57 0.0 0.0 0.0 0.6798926693708353 0.0 58-59 0.0 0.0 0.0 0.8536613540425559 0.0 60-61 0.0 0.0 0.0 1.0564739974058464 0.0 62-63 0.0 0.0 0.0 1.3503790566654235 0.0 64-65 0.0 0.0 0.0 1.5561621048948973 0.0 66-67 0.0 0.0 0.0 1.819538003029813 0.0 68-69 0.0 0.0 0.0 2.160309450178059 0.0 70-71 0.0 0.0 0.0 2.3913409397700907 0.0 72-73 0.0 0.0 0.0 2.7199007224684726 0.0 74-75 0.0 0.0 0.0 3.1240408067619616 0.0 76-77 0.0 0.0 0.0 3.424381743231603 0.0 78-79 0.0 0.0 0.0 3.687262573888821 0.0 80-81 0.0 0.0 0.0 4.0758905438811315 0.0 82-83 0.0 0.0 0.0 4.502968754641258 0.0 84-85 0.0 0.0 0.0 5.064375274349894 0.0 86-87 0.0 0.0 0.0 5.910610616226991 0.0 88-89 0.0 0.0 0.0 6.761301565403365 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAATGCG 20 0.0020692 71.27947 2 GCCACGA 25 0.005019338 56.99535 7 CCTAGAG 50 0.001613276 38.00317 3 AGATATA 80 1.0755943E-5 35.622093 8 ACTCTAT 55 0.0025714182 34.559746 1 ACTATAC 55 0.0025755868 34.548332 3 CCTACGT 70 2.0448008E-4 33.942608 2 TCCTACG 70 2.0448008E-4 33.942608 1 CATGGGT 180 5.456968E-12 31.664082 4 CTATGTC 125 2.1434789E-7 30.402536 3 ATGGGGG 295 0.0 28.980684 5 GGTGTGC 255 0.0 27.938896 8 TAGTAGC 85 6.3992623E-4 27.938896 9 GATATAG 120 5.3679432E-6 27.706068 9 GTATAGG 105 6.8699555E-5 27.154087 1 GACTAAC 35 0.008385082 27.14512 52-53 TAGACTG 70 0.0083853295 27.14064 5 AGGGTGT 235 5.456968E-12 26.27445 6 GTACATG 2355 0.0 25.222746 1 GTCTATC 95 0.0012238743 25.002087 3 >>END_MODULE