FastQCFastQC Report
Thu 26 May 2016
SRR936555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115035
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29350.26322043702664044No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20460.18349199800903113No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16430.147349634764828No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16270.145914702229078No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12280.1101310721188124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC702.0510379E-433.9349178
GTACATG65700.030.9480821
TACATGG67400.029.7445832
ACATGGG67850.028.9871713
ATACCGT1205.373664E-627.7135166
ATGGGTA5650.024.385095
CATGGGA34050.023.7195594
ATGGGAG15250.023.3650255
CATGGGG29700.022.7146854
CTTACAC3150.022.6222633
CATGGGT11050.022.3571224
TACACCG1754.042504E-621.7183465
CCGTACC1100.002873443821.5959159
GTGTATA3451.8189894E-1220.655111
GAGTACT14300.019.76723912-13
TATACAG5050.019.756175
ATGGGAT13950.019.7527985
ATGGGGA15950.019.3609875
AGTACAT45700.018.5037232
GTATATA2854.2648026E-818.335941
Produced by FastQC (version 0.11.5)