FastQCFastQC Report
Thu 26 May 2016
SRR936555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1115035
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44350.39774536225320284No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16450.14752900133179675No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13180.11820256763240616No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13030.11685731838014053No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11710.10501912496020305No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11580.10385324227490617No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT11570.1037635589914218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.10250799302264053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67150.032.2596171
TACATGG68900.031.0251672
ACATGGG68500.030.024743
ATAACGG650.005846048729.2286728
CATGGGG30500.026.3177834
TATACCG950.001227044624.9982075
GGGCGTA2101.2660166E-924.8791689
ATACCGT1151.2823439E-424.7808326
CATGGGA35300.024.4883274
GTAAGGT4100.023.169074
TAAGGTG4150.022.8899235
GTGTAAG3900.021.9254381
ATGGGAG15850.021.8754025
TACGCTC1100.002878510421.5893615
CATGGGT10800.021.1095964
GGGTACC4800.020.7797577
ATGGGTA6000.020.5818565
GTGCATA6500.020.4637411
GCATAAG4900.020.3565943
ATGGGGA17500.020.3556825
Produced by FastQC (version 0.11.5)