##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936555_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1115035 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19787629984709 33.0 31.0 34.0 31.0 34.0 2 32.32755563726699 34.0 31.0 34.0 31.0 34.0 3 32.461080593882706 34.0 31.0 34.0 31.0 34.0 4 36.117530839839105 37.0 35.0 37.0 35.0 37.0 5 36.01088216961799 37.0 35.0 37.0 35.0 37.0 6 36.0627971319286 37.0 35.0 37.0 35.0 37.0 7 35.995866497464206 37.0 35.0 37.0 35.0 37.0 8 35.96315631347895 37.0 35.0 37.0 35.0 37.0 9 37.64528378032976 39.0 37.0 39.0 35.0 39.0 10-11 37.54206280520343 39.0 37.0 39.0 35.0 39.0 12-13 37.4405875151901 39.0 37.0 39.0 35.0 39.0 14-15 38.638044994103325 40.0 38.0 41.0 34.0 41.0 16-17 38.60668992453152 40.0 38.0 41.0 34.5 41.0 18-19 38.68918419601178 40.0 38.0 41.0 34.0 41.0 20-21 38.66960902572565 40.0 38.0 41.0 34.0 41.0 22-23 38.582035093068825 40.0 38.0 41.0 34.0 41.0 24-25 38.460998533678314 40.0 38.0 41.0 34.0 41.0 26-27 38.27139506831624 40.0 38.0 41.0 34.0 41.0 28-29 38.355903177927146 40.0 38.0 41.0 34.0 41.0 30-31 38.30997367795629 40.0 38.0 41.0 34.0 41.0 32-33 38.192689915563186 40.0 38.0 41.0 34.0 41.0 34-35 38.04145430412498 40.0 37.5 41.0 33.0 41.0 36-37 37.894891191756315 40.0 37.0 41.0 33.0 41.0 38-39 37.72282618931244 40.0 37.0 41.0 33.0 41.0 40-41 37.5421807387212 40.0 37.0 41.0 33.0 41.0 42-43 37.32744173949696 39.5 36.5 41.0 32.0 41.0 44-45 37.11928011228347 39.0 36.0 41.0 31.5 41.0 46-47 36.85886496836422 39.0 36.0 40.5 31.0 41.0 48-49 36.548115081589366 39.0 35.0 40.0 30.5 41.0 50-51 36.28364625325663 39.0 35.0 40.0 30.0 41.0 52-53 36.03468366463833 38.0 35.0 40.0 29.5 41.0 54-55 35.705426287067226 38.0 34.5 40.0 29.0 41.0 56-57 35.34936347289547 38.0 34.0 40.0 28.0 41.0 58-59 34.98274807517253 37.5 34.0 40.0 28.0 41.0 60-61 34.577452725699196 37.0 33.5 39.5 27.0 41.0 62-63 34.24713843063222 36.0 33.0 39.0 26.0 40.0 64-65 33.9218136650419 36.0 33.0 39.0 26.0 40.0 66-67 33.47032290466218 35.0 32.5 38.5 26.0 40.0 68-69 32.99764581380853 35.0 32.0 38.0 25.5 40.0 70-71 32.49843099095544 35.0 31.5 37.0 24.5 39.0 72-73 31.95271897294704 34.5 31.0 36.5 23.5 39.0 74-75 31.437488060912884 34.0 30.5 36.0 22.0 38.5 76-77 30.03888218755465 32.5 28.5 34.5 20.5 36.5 78-79 30.85268265121723 34.0 30.0 35.0 22.0 37.0 80-81 30.71831870748452 34.0 30.0 35.0 20.5 36.5 82-83 30.363193083625177 34.0 30.0 35.0 20.0 36.0 84-85 29.970907191254085 34.0 30.0 35.0 18.5 36.0 86-87 29.594590304340223 34.0 29.0 35.0 17.5 35.0 88-89 29.145879725748518 33.0 29.0 35.0 9.5 35.0 90-91 28.629094153995165 33.0 29.0 35.0 4.5 35.0 92-93 28.10534512369567 33.0 27.5 34.0 2.0 35.0 94-95 27.397808140551643 32.5 26.5 34.0 2.0 35.0 96-97 26.620822216342987 32.0 25.0 34.0 2.0 35.0 98-99 25.472321496634635 32.0 23.5 34.0 2.0 35.0 100-101 21.69578578250907 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21412475673010078 1101 2 0.32299330674599247 1101 3 0.26312670591912024 1101 4 0.1752652426073169 1101 5 0.11014632417526826 1101 6 0.1316832507530421 1101 7 0.161709281606349 1101 8 0.18821524588744154 1101 9 0.2052270079788343 1101 10-11 0.26127913428729954 1101 12-13 0.25191015321885146 1101 14-15 0.2975853285985153 1101 16-17 0.3712826205620914 1101 18-19 0.2698247305283914 1101 20-21 0.2218759617358117 1101 22-23 0.2660918392989302 1101 24-25 0.2675568052005417 1101 26-27 0.10702127770267822 1101 28-29 0.17107179788613536 1101 30-31 0.24464226795075916 1101 32-33 0.26576328740810595 1101 34-35 0.3097990942031714 1101 36-37 0.24511906007494133 1101 38-39 0.16192072440392025 1101 40-41 0.21046999264786592 1101 42-43 0.31210115975753894 1101 44-45 0.42607276215378675 1101 46-47 0.2744840185829176 1101 48-49 0.21896320050063878 1101 50-51 0.2200947405712128 1101 52-53 0.11663411646090793 1101 54-55 0.14770372615032556 1101 56-57 0.09212019733043775 1101 58-59 0.10220522955903277 1101 60-61 -0.050582394759835836 1101 62-63 0.11772911061864733 1101 64-65 0.10996105038811521 1101 66-67 0.06029347956189213 1101 68-69 0.12707581514322186 1101 70-71 0.04951193845169399 1101 72-73 0.10320011169065779 1101 74-75 0.18243352918398514 1101 76-77 0.12903222360909794 1101 78-79 0.16720610591041307 1101 80-81 0.1803843984830813 1101 82-83 0.0700916001006 1101 84-85 -0.04607330540641286 1101 86-87 0.027121143170088402 1101 88-89 -0.19654161622961652 1101 90-91 0.15236884599163503 1101 92-93 0.23003787944919196 1101 94-95 0.291978143557575 1101 96-97 0.23237831296016154 1101 98-99 -0.17929787098436734 1101 100-101 0.19761548526928507 1102 1 0.13841151193251733 1102 2 0.33139523938132953 1102 3 0.22185565988457512 1102 4 0.1183032447411918 1102 5 -0.0058868290216480545 1102 6 0.05734922058380931 1102 7 0.09600445088543097 1102 8 0.03117344440760661 1102 9 0.1315995000201582 1102 10-11 0.1471554126631176 1102 12-13 0.22261680554399987 1102 14-15 0.2781277763141361 1102 16-17 0.34454819310964524 1102 18-19 0.28435506023875945 1102 20-21 0.12568333142605326 1102 22-23 0.19277747378102816 1102 24-25 0.13601854717464334 1102 26-27 0.109370713439084 1102 28-29 0.20212971622621723 1102 30-31 0.24148677234429528 1102 32-33 0.09573605008776553 1102 34-35 0.13260514539891943 1102 36-37 0.29809174468586264 1102 38-39 0.2008924288182854 1102 40-41 0.2149038640773071 1102 42-43 0.1592451445601526 1102 44-45 0.1416208047966805 1102 46-47 0.05112606467817926 1102 48-49 0.12083316398180699 1102 50-51 -0.0014875829283482744 1102 52-53 0.05418128299891123 1102 54-55 -0.008486909069084447 1102 56-57 0.08767418187282772 1102 58-59 -0.0409253707896724 1102 60-61 -0.0211578482091781 1102 62-63 -0.2140739305929813 1102 64-65 -0.2036842832690624 1102 66-67 -0.13100380624781138 1102 68-69 -0.3371159997817941 1102 70-71 -0.644007053940939 1102 72-73 -0.5148819125453592 1102 74-75 -0.4015231253097369 1102 76-77 -0.4705772157705148 1102 78-79 -0.43468163989773245 1102 80-81 -0.44508661632395885 1102 82-83 -0.39001201448264666 1102 84-85 -0.5502486825218575 1102 86-87 -0.48792747631413746 1102 88-89 -0.7437014316403889 1102 90-91 -0.7658344016684673 1102 92-93 -0.6422453279621365 1102 94-95 -0.674172112768165 1102 96-97 -0.8003066882976135 1102 98-99 -1.0387136131145986 1102 100-101 -0.8478990311815338 1103 1 0.1296386291534759 1103 2 0.1713492804865524 1103 3 0.1444611388624324 1103 4 0.1036234655501147 1103 5 0.1418122801565076 1103 6 0.0687238629109217 1103 7 0.15874220898992064 1103 8 0.189316417815526 1103 9 -0.01534007213493993 1103 10-11 0.263495769949337 1103 12-13 0.2139023840994625 1103 14-15 0.2741924538837637 1103 16-17 0.10261447629451226 1103 18-19 0.11236089348309264 1103 20-21 0.09302830301476206 1103 22-23 0.21948555746451603 1103 24-25 0.10061445506535449 1103 26-27 0.15659182065072486 1103 28-29 0.18464011037529104 1103 30-31 0.3491472325961169 1103 32-33 0.26117702347359284 1103 34-35 0.18523968012035397 1103 36-37 0.18043715818701855 1103 38-39 0.04842480811173289 1103 40-41 0.21329180428655548 1103 42-43 0.20612929352883924 1103 44-45 0.32065288764052724 1103 46-47 0.1734690660738849 1103 48-49 0.22462228704338116 1103 50-51 0.08566906030468147 1103 52-53 0.16166267664347345 1103 54-55 0.0446820635212859 1103 56-57 -0.013699613455429471 1103 58-59 -0.2206276875156945 1103 60-61 -0.21535075035871643 1103 62-63 -0.19457730569931897 1103 64-65 -0.16455431681757915 1103 66-67 -0.22818357623238228 1103 68-69 -0.153435550356825 1103 70-71 -0.4206994865584974 1103 72-73 -0.06067324885178138 1103 74-75 -0.22196811273364858 1103 76-77 -0.36967893665124407 1103 78-79 -0.3529577578441625 1103 80-81 -0.04094249026107022 1103 82-83 -0.31362698721296667 1103 84-85 -0.15506847430744486 1103 86-87 -0.13256542112663894 1103 88-89 -0.32052560940703856 1103 90-91 -0.4183149114881317 1103 92-93 -0.4204637395078379 1103 94-95 -0.3630427318506797 1103 96-97 -0.19853931469377883 1103 98-99 -0.32209503511521476 1103 100-101 -0.3626322081269606 1104 1 0.21737794400726074 1104 2 0.30178581390855186 1104 3 0.14441260990923155 1104 4 0.06502023207628582 1104 5 0.03197886869499911 1104 6 0.0571016948850982 1104 7 0.03567794633057275 1104 8 0.12560306491917572 1104 9 0.18635142001679128 1104 10-11 0.10884483767716802 1104 12-13 0.19337102303784803 1104 14-15 0.1856864848225186 1104 16-17 0.08435519952047343 1104 18-19 0.07645927715919498 1104 20-21 0.19317709072021216 1104 22-23 0.12838638699985694 1104 24-25 -0.03998321513187619 1104 26-27 0.13329947191134295 1104 28-29 0.10048935673098214 1104 30-31 0.10686385242028962 1104 32-33 0.22741016482500953 1104 34-35 0.08844713416756633 1104 36-37 0.09338034627079139 1104 38-39 0.1377098217514643 1104 40-41 0.14784638077335188 1104 42-43 0.16191631957133978 1104 44-45 0.13948243124121262 1104 46-47 0.12579711353726708 1104 48-49 0.03315698111479293 1104 50-51 0.3121996325126588 1104 52-53 0.27068503506799146 1104 54-55 0.20172691872947723 1104 56-57 0.23760498936090357 1104 58-59 0.3171712043721229 1104 60-61 0.22919098144344474 1104 62-63 0.2989636971360099 1104 64-65 0.2868805914007524 1104 66-67 0.13569271004300987 1104 68-69 0.11225065936672252 1104 70-71 0.016988821267879928 1104 72-73 0.24177261038947861 1104 74-75 0.4189418980666275 1104 76-77 0.3330512816051403 1104 78-79 0.4296862234298544 1104 80-81 0.12018705726323375 1104 82-83 0.18555834589183462 1104 84-85 0.23124729016812395 1104 86-87 0.41575444456173827 1104 88-89 0.4112233740662745 1104 90-91 0.26845650888204986 1104 92-93 0.1832390196212863 1104 94-95 0.27517495457794183 1104 96-97 0.29706313612160784 1104 98-99 -0.036508340721649546 1104 100-101 -0.03537824475702678 1105 1 0.08208587043213811 1105 2 0.16052810392471883 1105 3 0.12494668851451962 1105 4 0.1008076939633824 1105 5 0.0686022189014821 1105 6 0.04140097892397421 1105 7 -0.028108066259662223 1105 8 -0.0177576087072282 1105 9 0.046253783175835395 1105 10-11 0.06260082609126982 1105 12-13 0.03949447177109988 1105 14-15 0.20333125029704746 1105 16-17 0.16323010867455423 1105 18-19 0.17662814990710984 1105 20-21 -0.029969070020378297 1105 22-23 -0.03696959124373933 1105 24-25 0.03501789726702498 1105 26-27 -0.05591269540192911 1105 28-29 -0.01696566527333232 1105 30-31 -0.10065416314481723 1105 32-33 -0.03249601227313548 1105 34-35 -0.20733337787494577 1105 36-37 -0.11215865867249875 1105 38-39 -0.20448791827772794 1105 40-41 -0.04656492713231586 1105 42-43 -0.009558912072492376 1105 44-45 -0.1259857403775726 1105 46-47 -0.13940408234453372 1105 48-49 -0.12572695436183068 1105 50-51 0.061510474905873025 1105 52-53 0.005385312426120947 1105 54-55 0.05348811105159257 1105 56-57 0.09612109159989757 1105 58-59 0.08888470126694159 1105 60-61 -0.08392494323549471 1105 62-63 0.1334641664624101 1105 64-65 -0.05206735468710377 1105 66-67 0.11880490553705414 1105 68-69 -0.0309627538333217 1105 70-71 -0.117491201107228 1105 72-73 -0.08662396723583754 1105 74-75 -0.015670234921191906 1105 76-77 -0.2903142865764359 1105 78-79 -0.2624632067040764 1105 80-81 -0.1114965002096504 1105 82-83 -0.015008235950002558 1105 84-85 0.18871233435684687 1105 86-87 0.055681513570728924 1105 88-89 -0.05482250338338801 1105 90-91 -0.23003327717715294 1105 92-93 -0.06913780603720099 1105 94-95 0.040316292804416065 1105 96-97 0.27607587679705503 1105 98-99 0.38752328954661763 1105 100-101 0.17545418982861705 1106 1 -0.003253546299873733 1106 2 0.1342619995139387 1106 3 0.10388442929448871 1106 4 0.07188118027514889 1106 5 0.2044088825037207 1106 6 0.042747642126506946 1106 7 0.06677849308933048 1106 8 0.04313966516680523 1106 9 0.14771495095796183 1106 10-11 0.10080996976395085 1106 12-13 0.018297311108476322 1106 14-15 0.11943019448018077 1106 16-17 0.20137429980080412 1106 18-19 0.2349428987788187 1106 20-21 -0.04812169028250679 1106 22-23 0.07558200341474475 1106 24-25 0.13228742153224005 1106 26-27 0.06185420666138697 1106 28-29 0.2577336426440624 1106 30-31 0.16378587536821954 1106 32-33 0.16545319189653185 1106 34-35 0.0867599962599499 1106 36-37 0.18740513060615882 1106 38-39 0.32268810958897376 1106 40-41 0.16497258487362387 1106 42-43 0.4158579304460943 1106 44-45 0.2586638274087889 1106 46-47 0.1374536486543505 1106 48-49 0.020234349203448687 1106 50-51 0.04392567509490419 1106 52-53 0.12217616287538391 1106 54-55 0.16168906071072797 1106 56-57 0.1679030603027627 1106 58-59 0.16030752059540276 1106 60-61 0.05281192312449434 1106 62-63 0.05654124779474756 1106 64-65 -0.009295368103181545 1106 66-67 -0.19329501910623748 1106 68-69 -0.12762918929634992 1106 70-71 -0.14469691316936206 1106 72-73 -0.07811724097349426 1106 74-75 0.06763125892859279 1106 76-77 -0.17178147349046924 1106 78-79 -0.3868085379385491 1106 80-81 -0.3180896833076474 1106 82-83 -0.21778714688683465 1106 84-85 0.02687821551895908 1106 86-87 0.2685066142912653 1106 88-89 0.3363187922112445 1106 90-91 0.21168048253487015 1106 92-93 -0.0861586061350117 1106 94-95 -0.07255653775114013 1106 96-97 0.46692468020068745 1106 98-99 1.0580498812954708 1106 100-101 0.6966989627650975 1107 1 0.17285749562798003 1107 2 0.20619122525432942 1107 3 0.20361156048122098 1107 4 0.13888663700618054 1107 5 0.10313448384425783 1107 6 0.09948293356833204 1107 7 0.043635294710668404 1107 8 0.16016999560970646 1107 9 0.14057093700545664 1107 10-11 0.10985193503882584 1107 12-13 0.19383474136690637 1107 14-15 0.2582538969660675 1107 16-17 0.25566932202583814 1107 18-19 0.22685781326293863 1107 20-21 0.16381427168490603 1107 22-23 0.20400939766275883 1107 24-25 0.23519974282704226 1107 26-27 0.36726790068827597 1107 28-29 0.266595588058685 1107 30-31 0.3543330260538795 1107 32-33 0.33654271209520914 1107 34-35 0.3798116813168164 1107 36-37 0.3030891834919913 1107 38-39 0.3892809401528581 1107 40-41 0.2891478971177506 1107 42-43 0.2679641950727074 1107 44-45 0.28160119662823035 1107 46-47 0.3904596254427659 1107 48-49 0.3399134879987642 1107 50-51 0.25200346948383867 1107 52-53 0.17621180164585581 1107 54-55 0.2256132097620096 1107 56-57 -0.008707068943749618 1107 58-59 0.03761592042533124 1107 60-61 -0.003051659244910354 1107 62-63 -0.10944190260442355 1107 64-65 -0.010647775368759937 1107 66-67 -0.12260915634331582 1107 68-69 -0.06936469689106417 1107 70-71 -0.04707001368560526 1107 72-73 -0.14435648414161406 1107 74-75 0.0906473465098685 1107 76-77 0.060665460977784136 1107 78-79 0.15163344501569753 1107 80-81 0.19368161533239814 1107 82-83 0.21447925153518455 1107 84-85 0.03371025957027385 1107 86-87 0.33207814379548495 1107 88-89 0.3882174210277114 1107 90-91 0.19251223881249757 1107 92-93 0.08315813784125936 1107 94-95 0.3979405299898815 1107 96-97 0.7695348391278962 1107 98-99 0.9781236442719035 1107 100-101 0.9615123712120912 1108 1 0.06110693948982515 1108 2 0.17316001085211497 1108 3 -3.464237423145278E-5 1108 4 0.052069725883697515 1108 5 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-0.8871663795689457 2316 72-73 -0.6653859622359235 2316 74-75 -0.8995220225238576 2316 76-77 -1.0066548275161757 2316 78-79 -1.1706817164407077 2316 80-81 -1.0397904800434397 2316 82-83 -1.1994550792635188 2316 84-85 -1.0676416570103697 2316 86-87 -1.2527952412308032 2316 88-89 -1.5346877162725434 2316 90-91 -2.054715754381352 2316 92-93 -1.638610599562142 2316 94-95 -1.6260038996498274 2316 96-97 -1.4832738201754978 2316 98-99 -0.6937567323260403 2316 100-101 -1.0227451656193516 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 9.0 5 50.0 6 60.0 7 163.0 8 301.0 9 566.0 10 832.0 11 1362.0 12 1777.0 13 2380.0 14 3093.0 15 4051.0 16 4951.0 17 6277.0 18 5730.0 19 4886.0 20 5049.0 21 5576.0 22 5967.0 23 6923.0 24 8162.0 25 9959.0 26 12345.0 27 15295.0 28 19500.0 29 24587.0 30 30956.0 31 39631.0 32 51581.0 33 68575.0 34 94466.0 35 134728.0 36 196093.0 37 237049.0 38 109119.0 39 2980.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.91993427118875 17.78247781832011 12.905288801346149 23.392299109144986 2 16.55368437669294 19.707262557806633 38.401272194337906 25.33778087116252 3 21.021528714519416 23.51342091668341 28.216661165800875 27.248389202996297 4 13.547503569404288 15.2156171302707 35.546513463290744 35.69036583703427 5 14.699008053956126 37.82426590611491 32.759332005955265 14.717394033973704 6 34.91083239539566 33.716878842368175 16.89399884308564 14.47828991915052 7 32.06978101954116 31.455268956647842 18.775173066937153 17.699776956873844 8 28.824301757646094 33.08944999793725 18.605865038465886 19.480383205950776 9 28.30772128229159 16.080212728748425 18.628204495823 36.983861493136985 10-11 26.6712704085522 25.164680929298182 26.149268857031394 22.014779805118227 12-13 27.695677714152474 22.23885348890394 26.552081324801463 23.51338747214213 14-15 24.27795664690636 24.039084150202235 24.51727756203869 27.16568164085271 16-17 24.203793664609698 27.290053143950555 25.17918314102228 23.32697005041747 18-19 24.018457109417565 26.226404518314734 26.866349631283814 22.888788740983887 20-21 25.158291705155918 25.182685667650194 26.171627669210697 23.48739495798319 22-23 24.55296065892368 25.128819346197258 26.203902045728793 24.11431794915027 24-25 24.586961044924433 25.29245902345053 26.657671376862286 23.462908554762752 26-27 24.366073142164275 25.52437862881311 26.65672078160845 23.45282744741416 28-29 23.90715264864002 25.95179790401104 26.629983197829304 23.511066249519633 30-31 24.01173062608181 25.52868532685219 26.422607463476318 24.036976583589677 32-33 24.225829350837927 25.569974802317702 26.351062594199824 23.853133252644547 34-35 24.69810093944082 25.34669626241564 26.487388175153026 23.467814622990517 36-37 24.80206704070944 25.282101551177373 26.3478185222919 23.56801288582129 38-39 24.502026513741637 25.30299048673102 26.6334759082988 23.561507091228545 40-41 24.29190500020627 25.56325636707042 26.425517069434655 23.719321563288652 42-43 24.221140635033382 25.581151325347882 26.192705474660727 24.005002564958012 44-45 24.436167269293048 25.302729509619226 26.178305360497333 24.08279786059039 46-47 24.861506589479255 25.840534153636437 25.691749586335856 23.60620967054846 48-49 24.765811165549646 25.790771278336962 25.66494382606933 23.778473730044063 50-51 24.404856927103044 25.90912678269386 26.06176896323494 23.62424732696816 52-53 24.691757725338825 25.83082817354121 25.75858809433272 23.71882600678725 54-55 24.01959223646644 25.69442980861113 25.911284279799666 24.374693675122767 56-57 24.546653589301677 25.959600987888187 25.603964827449193 23.889780595360943 58-59 24.9488122560006 25.249167498495535 25.555038371749927 24.24698187375394 60-61 24.424499386775427 25.622473391434585 26.422378324809475 23.530648896980512 62-63 24.203242141835073 25.587324876494854 26.411514621296472 23.7979183603736 64-65 24.12028145253616 25.964673590125216 25.994718392755527 23.920326564583096 66-67 24.222587832877966 25.857342787791715 25.720350896545785 24.199718482784537 68-69 24.24228971574962 25.6955813816876 25.983244254253623 24.078884648309156 70-71 24.355834741044646 25.675398736938774 25.99504300264711 23.973723519369464 72-73 24.25638792714169 25.515520664099583 26.00560441644877 24.222486992309957 74-75 24.103782723029624 26.272372919663773 25.567507112337257 24.056337244969345 76-77 24.419408677996138 25.723771711357664 25.784218678112726 24.072600932533472 78-79 24.370249855152533 25.67916869213949 25.763386506853124 24.187194945854852 80-81 24.461944046216647 26.018388184136548 25.62619421628246 23.893473553364345 82-83 24.27801990391984 25.910297383916596 25.730567489965306 24.081115222198257 84-85 24.081541138275547 25.824693727466773 25.699090599271763 24.39467453498592 86-87 24.179451432538006 25.85738609178306 25.585779415200378 24.37738306047856 88-89 24.07305764073865 25.955238069613724 25.371745544883005 24.59995874476462 90-91 23.977296066857946 25.48277473382477 25.8595592132819 24.680369986035387 92-93 24.03056628157624 25.689685247657103 25.89219291680549 24.387555553961164 94-95 23.7019867609062 25.656327043822102 26.08438689148553 24.557299303786174 96-97 23.634491699128322 25.55169867093679 26.122578379932083 24.6912312500028 98-99 23.552136017804 26.10264921004403 25.437644979218057 24.907569792933913 100-101 23.265912866838413 26.285058306259767 25.476281762393864 24.972747064507956 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 40.0 1 31.5 2 22.5 3 28.5 4 32.0 5 33.5 6 56.0 7 113.5 8 172.0 9 201.5 10 221.5 11 220.0 12 209.0 13 223.5 14 261.0 15 285.0 16 312.0 17 361.5 18 403.5 19 426.0 20 475.5 21 524.0 22 575.5 23 707.5 24 833.5 25 1025.5 26 1301.5 27 1704.5 28 2295.0 29 3120.5 30 4383.5 31 6091.5 32 8416.0 33 10477.5 34 12460.0 35 15234.5 36 19100.5 37 22253.5 38 24451.5 39 26388.0 40 28041.5 41 30177.0 42 31847.0 43 34500.5 44 37675.5 45 40465.5 46 43024.5 47 46795.5 48 55878.0 49 56402.0 50 49632.5 51 47137.5 52 45830.5 53 46065.0 54 45279.5 55 42622.0 56 38460.5 57 35360.5 58 34780.0 59 32281.0 60 26877.0 61 21885.5 62 17888.0 63 13993.5 64 10306.5 65 7791.0 66 5969.5 67 4774.5 68 3797.5 69 2774.5 70 2184.0 71 1754.5 72 1493.5 73 1366.5 74 1156.5 75 995.5 76 756.0 77 474.5 78 341.0 79 247.5 80 162.0 81 123.0 82 93.0 83 57.5 84 31.5 85 15.5 86 5.5 87 2.5 88 1.0 89 1.0 90 2.0 91 1.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013542175806140614 2 0.009954844466765617 3 0.005291313725578121 4 2.690498504531248E-4 5 0.004932580591640622 6 0.0 7 3.5873313393749973E-4 8 0.0018833489531718734 9 0.0 10-11 0.0 12-13 0.0 14-15 4.484164174218746E-4 16-17 0.003990906115054684 18-19 0.0015694574609765612 20-21 4.484164174218746E-4 22-23 4.0357477567968717E-4 24-25 6.277829843906245E-4 26-27 8.968328348437493E-5 28-29 4.4841641742187466E-5 30-31 4.484164174218746E-4 32-33 0.004618689099445309 34-35 8.968328348437492E-4 36-37 9.865161183281242E-4 38-39 0.005919096709968746 40-41 8.968328348437493E-5 42-43 6.277829843906245E-4 44-45 0.004887738949898433 46-47 0.0 48-49 0.001434932535749999 50-51 8.071495513593743E-4 52-53 2.690498504531248E-4 54-55 1.345249252265624E-4 56-57 0.011793351778195304 58-59 0.0019730322366562484 60-61 0.002466290295820311 62-63 4.4841641742187466E-5 64-65 0.00300438999672656 66-67 4.932580591640621E-4 68-69 0.0013004076105234364 70-71 0.004080589398539059 72-73 0.001928190594914061 74-75 0.006367513127390621 76-77 7.174662678749995E-4 78-79 0.00618814656042187 80-81 0.0022869237288515604 82-83 0.0025559735793046854 84-85 0.001345249252265624 86-87 3.1389149219531224E-4 88-89 0.0022420820871093734 90-91 0.006636562977843746 92-93 0.0013900908940078114 94-95 3.5873313393749973E-4 96-97 1.345249252265624E-4 98-99 4.0357477567968717E-4 100-101 0.0015694574609765612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1115035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.15697298514435 #Duplication Level Percentage of deduplicated Percentage of total 1 73.16335998205903 35.96489310140467 2 14.060695045730093 13.823624130306145 3 5.046264500491284 7.441772631796291 4 2.437809949845689 4.793414313899225 5 1.3568622577581375 3.3349620674589366 6 0.8309538444516129 2.4508305410165865 7 0.5772026460279338 1.9861474414424287 8 0.4040343400783772 1.5888884112242732 9 0.29908161177834125 1.3231752039487217 >10 1.630405793272937 14.763145548530757 >50 0.12603671606254774 4.2593084927198746 >100 0.06107599651275387 5.516072100953127 >500 0.004571555831792467 1.461603897788853 >1k 0.001645760099445288 1.292162117510133 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4435 0.39774536225320284 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1645 0.14752900133179675 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1318 0.11820256763240616 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1303 0.11685731838014053 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1171 0.10501912496020305 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1158 0.10385324227490617 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1157 0.1037635589914218 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1143 0.10250799302264053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.968328348437493E-5 0.0 20-21 0.0 0.0 0.0 8.968328348437493E-5 0.0 22-23 0.0 0.0 0.0 8.968328348437493E-5 0.0 24-25 0.0 0.0 0.0 8.968328348437493E-5 0.0 26-27 0.0 0.0 0.0 3.5873313393749973E-4 0.0 28-29 0.0 0.0 0.0 9.865161183281242E-4 0.0 30-31 0.0 0.0 0.0 0.0023766070123359354 0.0 32-33 0.0 0.0 0.0 0.003766697906343747 0.0 34-35 0.0 0.0 0.0 0.005022263875124996 0.0 36-37 0.0 0.0 0.0 0.007174662678749994 0.0 38-39 0.0 0.0 0.0 0.009640952974570304 0.0 40-41 0.0 0.0 0.0 0.01448385028272655 0.0 42-43 0.0 0.0 0.0 0.021030729977085924 0.0 44-45 0.0 0.0 0.0 0.03887770339047653 0.0 46-47 0.0 0.0 0.0 0.07663436573739837 0.0 48-49 0.0 0.0 0.0 0.10304609272354678 0.0 50-51 0.0 0.0 0.0 0.1384261480581327 0.0 52-53 0.0 0.0 0.0 0.1786042590591327 0.0 54-55 4.4841641742187466E-5 0.0 0.0 0.22761617348334356 0.0 56-57 8.968328348437493E-5 0.0 0.0 0.28034994417215603 0.0 58-59 8.968328348437493E-5 0.0 0.0 0.36308277318649196 0.0 60-61 8.968328348437493E-5 0.0 0.0 0.4687296811310856 0.0 62-63 8.968328348437493E-5 0.0 0.0 0.5938378615917885 0.0 64-65 8.968328348437493E-5 0.0 0.0 0.6866600599981166 0.0 66-67 8.968328348437493E-5 0.0 0.0 0.8489868031048353 0.0 68-69 8.968328348437493E-5 0.0 0.0 1.0477698009479521 0.0 70-71 8.968328348437493E-5 0.0 0.0 1.1850300663207882 0.0 72-73 8.968328348437493E-5 0.0 0.0 1.4314797293358503 0.0 74-75 8.968328348437493E-5 0.0 0.0 1.6211150322635612 0.0 76-77 8.968328348437493E-5 0.0 0.0 1.7776123619437953 0.0 78-79 8.968328348437493E-5 0.0 0.0 1.9869779872380688 0.0 80-81 8.968328348437493E-5 0.0 0.0 2.1867923428412563 0.0 82-83 8.968328348437493E-5 0.0 0.0 2.436246395853045 0.0 84-85 8.968328348437493E-5 0.0 0.0 2.8488791831646543 0.0 86-87 8.968328348437493E-5 4.4841641742187466E-5 0.0 3.3612397817108883 0.0 88-89 8.968328348437493E-5 8.968328348437493E-5 0.0 3.978664346859067 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6715 0.0 32.259617 1 TACATGG 6890 0.0 31.025167 2 ACATGGG 6850 0.0 30.02474 3 ATAACGG 65 0.0058460487 29.228672 8 CATGGGG 3050 0.0 26.317783 4 TATACCG 95 0.0012270446 24.998207 5 GGGCGTA 210 1.2660166E-9 24.879168 9 ATACCGT 115 1.2823439E-4 24.780832 6 CATGGGA 3530 0.0 24.488327 4 GTAAGGT 410 0.0 23.16907 4 TAAGGTG 415 0.0 22.889923 5 GTGTAAG 390 0.0 21.925438 1 ATGGGAG 1585 0.0 21.875402 5 TACGCTC 110 0.0028785104 21.589361 5 CATGGGT 1080 0.0 21.109596 4 GGGTACC 480 0.0 20.779757 7 ATGGGTA 600 0.0 20.581856 5 GTGCATA 650 0.0 20.463741 1 GCATAAG 490 0.0 20.356594 3 ATGGGGA 1750 0.0 20.355682 5 >>END_MODULE