FastQCFastQC Report
Thu 26 May 2016
SRR936554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296010
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33340.2572511014575505No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20610.15902655072105926No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17070.1317119466670782No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16670.12862555072877524No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13240.10215970555782748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG851.6275382E-533.5439151
GTACATG77550.032.9059071
TACATGG78400.032.001162
TAGATCG753.07017E-431.6815855
ACATGGG78350.031.1725273
CATGGGA39350.027.1707974
GTATAGG3050.026.4868621
ATGGGAG19600.026.1858025
CATGGGG36550.026.132044
ATGGGGG17550.021.391845
ATGGGTA5850.021.1210585
CATGGGT10350.020.201194
TACCTGG11400.020.0071052
ATGGGGA19800.019.4409735
GAGTACT17050.019.36792612-13
ATGGGAT14700.019.0736085
AGTACAT53000.018.9170952
TCCGTTG2302.0505322E-618.5942768
TAAGGCG4350.018.5719645
ATGGGCG2603.264704E-718.2778385
Produced by FastQC (version 0.11.5)