##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936554_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1296010 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.539031334634764 31.0 31.0 34.0 30.0 34.0 2 31.59770835101581 33.0 31.0 34.0 30.0 34.0 3 31.65804507681268 33.0 31.0 34.0 30.0 34.0 4 35.29619138741214 37.0 35.0 37.0 33.0 37.0 5 35.22299905093325 37.0 35.0 37.0 33.0 37.0 6 35.333220422681926 37.0 35.0 37.0 33.0 37.0 7 35.161066658436276 37.0 35.0 37.0 32.0 37.0 8 35.137282891335715 37.0 35.0 37.0 32.0 37.0 9 36.68223007538522 39.0 37.0 39.0 32.0 39.0 10-11 36.54358068224782 39.0 37.0 39.0 32.0 39.0 12-13 36.46975833519803 39.0 37.0 39.0 32.0 39.0 14-15 37.60303739940278 40.0 37.0 41.0 32.0 41.0 16-17 37.541694894329524 40.0 37.0 41.0 32.0 41.0 18-19 37.44997800942894 40.0 37.0 41.0 32.0 41.0 20-21 37.32434664855981 39.5 37.0 41.0 31.5 41.0 22-23 37.22467805032369 39.0 36.5 41.0 31.0 41.0 24-25 37.07286324951197 39.0 36.0 41.0 31.0 41.0 26-27 36.885171796513916 39.0 36.0 40.5 30.5 41.0 28-29 37.01710094829515 39.0 36.0 41.0 31.0 41.0 30-31 37.00830471987099 39.0 36.0 41.0 31.0 41.0 32-33 36.910704392713015 39.0 36.0 41.0 30.0 41.0 34-35 36.7672286479271 39.0 36.0 41.0 30.0 41.0 36-37 36.56265769554247 39.0 35.5 40.5 30.0 41.0 38-39 36.383753983379755 39.0 35.0 40.0 30.0 41.0 40-41 36.2553375359758 39.0 35.0 40.0 30.0 41.0 42-43 36.03312358700936 38.0 35.0 40.0 29.0 41.0 44-45 35.831835788304105 38.0 35.0 40.0 28.5 41.0 46-47 35.5880386725411 38.0 34.5 40.0 27.5 41.0 48-49 35.43250206402728 38.0 34.0 40.0 27.0 41.0 50-51 34.79017793072585 37.5 33.5 39.5 26.5 40.5 52-53 34.991966497172086 38.0 34.0 39.5 27.5 40.5 54-55 35.34656715611762 38.0 34.0 40.0 28.0 41.0 56-57 35.2530466585906 38.0 34.0 40.0 27.5 41.0 58-59 35.01367813519957 38.0 34.0 40.0 27.0 41.0 60-61 34.66533437241996 37.0 34.0 40.0 26.0 41.0 62-63 34.32898241525915 37.0 33.5 40.0 26.0 41.0 64-65 33.99254172421509 36.0 33.0 39.0 26.0 41.0 66-67 33.610629547611516 36.0 33.0 39.0 25.5 41.0 68-69 33.20814654207915 35.0 32.5 39.0 25.0 40.0 70-71 32.79941898596462 35.0 32.0 37.5 24.0 40.0 72-73 32.3916609439742 35.0 32.0 37.0 23.5 39.0 74-75 31.956420089351163 35.0 31.0 36.5 22.5 39.0 76-77 31.541435637070702 35.0 31.0 36.0 21.5 39.0 78-79 31.108813203601827 34.5 31.0 36.0 20.0 37.5 80-81 30.721237490451465 34.0 30.5 35.0 19.5 37.0 82-83 30.34921104003827 34.0 30.0 35.0 18.0 36.5 84-85 29.98582842724979 34.0 30.0 35.0 16.5 36.0 86-87 29.66062877601253 34.0 29.5 35.0 11.0 36.0 88-89 29.31447519695064 34.0 29.0 35.0 7.0 35.0 90-91 28.97914869484032 34.0 29.0 35.0 4.5 35.0 92-93 28.623617101719894 34.0 29.0 35.0 2.0 35.0 94-95 28.20864730982014 34.0 29.0 35.0 2.0 35.0 96-97 27.631618583189944 33.0 27.0 35.0 2.0 35.0 98-99 26.960820132560706 33.0 26.0 35.0 2.0 35.0 100-101 24.849632718883342 31.0 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3164660539073445 1101 2 0.3412769355382608 1101 3 0.4578970467874832 1101 4 0.3716074098434774 1101 5 0.3306082466364231 1101 6 0.39522533488274547 1101 7 0.35569360088199886 1101 8 0.24779320993477683 1101 9 0.522104536357638 1101 10-11 0.34807488731103575 1101 12-13 0.4991980053481484 1101 14-15 0.5720777450438348 1101 16-17 0.6432800451658451 1101 18-19 0.5506240607432815 1101 20-21 0.6358114377282149 1101 22-23 0.5893730563462753 1101 24-25 0.46550718890839704 1101 26-27 0.4796433525149766 1101 28-29 0.5775895103085489 1101 30-31 0.5469281749451156 1101 32-33 0.3822010742408679 1101 34-35 0.4523737029630084 1101 36-37 0.47237391684880947 1101 38-39 0.4358255740127319 1101 40-41 0.2388204707029402 1101 42-43 -0.011014936062395009 1101 44-45 0.16401852082931612 1101 46-47 0.30520831283957506 1101 48-49 0.33037520694929867 1101 50-51 0.49517910340875204 1101 52-53 0.44997718103018514 1101 54-55 0.47396215891222226 1101 56-57 0.4494676159139104 1101 58-59 0.3770939640035351 1101 60-61 0.28320285543722434 1101 62-63 0.3589792167751895 1101 64-65 0.4607823301194429 1101 66-67 0.31230205955378665 1101 68-69 0.35693254433974886 1101 70-71 0.3028780709286778 1101 72-73 0.26028705624332105 1101 74-75 0.21561325123218467 1101 76-77 0.1692475678051366 1101 78-79 0.0942147771874069 1101 80-81 -0.03825537085744202 1101 82-83 0.1482850948007588 1101 84-85 -0.04659980694576049 1101 86-87 -0.02944861039855695 1101 88-89 0.0543787701885492 1101 90-91 0.06569188183234687 1101 92-93 -0.15242240545029873 1101 94-95 -0.1466862557640205 1101 96-97 -0.200029518292844 1101 98-99 -0.5331001231022405 1101 100-101 -0.15461735576968394 1102 1 0.047909386874259496 1102 2 0.15051514593729465 1102 3 0.2044134807767115 1102 4 0.20381070030553872 1102 5 0.24843903334266315 1102 6 0.26238813580612685 1102 7 0.032352572704212434 1102 8 0.1357167011956335 1102 9 0.4220620315025556 1102 10-11 0.47062517861586173 1102 12-13 0.3915167184770283 1102 14-15 0.2550347638412447 1102 16-17 0.2031968675615019 1102 18-19 0.30564696206606357 1102 20-21 0.13845992990511746 1102 22-23 0.05583929063596571 1102 24-25 0.047466442874906534 1102 26-27 0.08230870006545388 1102 28-29 0.29668335076877383 1102 30-31 -0.01349101086705673 1102 32-33 -0.16402991766122454 1102 34-35 -0.18586674260512837 1102 36-37 -0.3631901885339204 1102 38-39 -0.39193889046273256 1102 40-41 -0.5549642935050443 1102 42-43 -0.3692315642548394 1102 44-45 -0.3661496664851853 1102 46-47 -0.22162764040508165 1102 48-49 -0.059193881761267164 1102 50-51 -0.16105078922984006 1102 52-53 -0.3828922631089071 1102 54-55 -0.6917965161088517 1102 56-57 -0.42218824132839927 1102 58-59 -0.5128114993360313 1102 60-61 -0.7253703005482635 1102 62-63 -0.5709533617305524 1102 64-65 -0.4446905363933311 1102 66-67 -0.3551828255731735 1102 68-69 -0.37774702035190444 1102 70-71 -0.2641165426802061 1102 72-73 -0.5816227308659769 1102 74-75 -0.784729718977772 1102 76-77 -0.7357086448657064 1102 78-79 -0.6753290284628086 1102 80-81 -0.5431994916298599 1102 82-83 -0.4910249341465125 1102 84-85 -0.5415930281542174 1102 86-87 -0.6972431214095138 1102 88-89 -1.0613582859838147 1102 90-91 -1.160253301561447 1102 92-93 -1.009374147782939 1102 94-95 -0.917113136420646 1102 96-97 -1.1856485868502453 1102 98-99 -1.0619733780214453 1102 100-101 -0.667511350126798 1103 1 0.1899260267703049 1103 2 0.26786897533925824 1103 3 0.21085046153194398 1103 4 0.1418968716254767 1103 5 0.2504047188209526 1103 6 -0.05845399573688326 1103 7 0.06213864837182115 1103 8 -0.013131710915516237 1103 9 0.25029992306048854 1103 10-11 0.15819248115997198 1103 12-13 0.27056051100169753 1103 14-15 0.36908362473628387 1103 16-17 0.36851297144354334 1103 18-19 0.31789226517566505 1103 20-21 0.21976892302898676 1103 22-23 0.11650670627843596 1103 24-25 0.2558452712240964 1103 26-27 0.24986157956156063 1103 28-29 0.3557283615070972 1103 30-31 0.11458986648739966 1103 32-33 0.11207895755158148 1103 34-35 0.05300916203130157 1103 36-37 0.19578300775789614 1103 38-39 0.11604040892905942 1103 40-41 0.12387022194671715 1103 42-43 0.08935706755587347 1103 44-45 0.16024421056246752 1103 46-47 0.3015142829888333 1103 48-49 0.2936192141858456 1103 50-51 -0.2545133434478686 1103 52-53 0.10759138274886482 1103 54-55 0.23125750038666837 1103 56-57 0.24862636760997248 1103 58-59 0.17885900244767328 1103 60-61 0.39243171115861486 1103 62-63 0.18291521858431992 1103 64-65 0.18767740022347112 1103 66-67 0.14001350233985477 1103 68-69 -0.011114719341840384 1103 70-71 0.10402574166590028 1103 72-73 -0.055044241811849304 1103 74-75 0.09618312911549509 1103 76-77 -0.09824338651417364 1103 78-79 0.13101034480386886 1103 80-81 0.21399248079339728 1103 82-83 0.17200852311374248 1103 84-85 0.055419641267715036 1103 86-87 0.05735939502885046 1103 88-89 0.049717983214328854 1103 90-91 0.15943566292687095 1103 92-93 0.14075602060125902 1103 94-95 -0.012977526682156793 1103 96-97 0.041883643172553775 1103 98-99 -0.20508904214339552 1103 100-101 0.038280654180564255 1104 1 0.2547533078904749 1104 2 0.2679779664660984 1104 3 0.16434971782778618 1104 4 0.30338966538862167 1104 5 0.27954061152239973 1104 6 0.2679455785565992 1104 7 0.3204155627689076 1104 8 0.42348674320562907 1104 9 0.31689991280249785 1104 10-11 0.43209199134102505 1104 12-13 0.42015083258963415 1104 14-15 0.3652689352968892 1104 16-17 0.17887321917396548 1104 18-19 0.424307675238758 1104 20-21 0.2934629528797288 1104 22-23 0.370068151628999 1104 24-25 0.1311097349877528 1104 26-27 0.06288569865680671 1104 28-29 0.08139769525075025 1104 30-31 0.12545051171487387 1104 32-33 0.2402348917978827 1104 34-35 0.26675358425010387 1104 36-37 0.31423232047267646 1104 38-39 0.16778394639636218 1104 40-41 0.007531810909121361 1104 42-43 0.10384487337215376 1104 44-45 0.007772035356019558 1104 46-47 0.2816730894718802 1104 48-49 0.16011630348163663 1104 50-51 7.550952862942495E-4 1104 52-53 0.2926759470408058 1104 54-55 0.5319768418238198 1104 56-57 0.4331082750829154 1104 58-59 0.5712879658779499 1104 60-61 0.3023136801794948 1104 62-63 0.27129099434469595 1104 64-65 0.35606388275923706 1104 66-67 0.39428659563626667 1104 68-69 0.6139686268139712 1104 70-71 0.5754764776765668 1104 72-73 0.16635039582470057 1104 74-75 -0.19231317800867487 1104 76-77 0.07294641191635165 1104 78-79 0.09951351195779168 1104 80-81 0.3999537825321262 1104 82-83 0.03392317665018396 1104 84-85 0.24341971820262742 1104 86-87 0.25246954017938705 1104 88-89 -0.041940348836757124 1104 90-91 0.026855971179426064 1104 92-93 -0.0660786847380237 1104 94-95 -0.05293078872247037 1104 96-97 -0.0737463255405828 1104 98-99 -0.3884281455952099 1104 100-101 -0.17088453790027103 1105 1 0.14854739362026592 1105 2 0.3875927250119453 1105 3 0.2733635539645185 1105 4 0.38437854221987067 1105 5 0.4858753917719909 1105 6 0.2821750956482916 1105 7 0.5403626598931623 1105 8 0.16928842524578158 1105 9 0.4472919366766206 1105 10-11 0.4489361313301714 1105 12-13 0.2940385157149805 1105 14-15 0.4225023861921642 1105 16-17 0.5244782110350599 1105 18-19 0.35818586297613564 1105 20-21 0.2059629528797231 1105 22-23 0.1363188298709943 1105 24-25 0.04184291458623335 1105 26-27 0.09656719621514043 1105 28-29 0.1574320142957859 1105 30-31 0.17412115740125245 1105 32-33 0.2245675016730857 1105 34-35 -0.09886632893491765 1105 36-37 0.09293213056491112 1105 38-39 -0.036227855014381305 1105 40-41 -0.018153213507595467 1105 42-43 0.045027727414478136 1105 44-45 0.06677569786280912 1105 46-47 -0.14211624856393001 1105 48-49 -0.07124560644783173 1105 50-51 -0.11114466054657868 1105 52-53 -0.01818270732706395 1105 54-55 0.04261845875273451 1105 56-57 0.15036275148010247 1105 58-59 0.02157960993654484 1105 60-61 0.10267653964774581 1105 62-63 -0.07143146903023734 1105 64-65 -0.012116393963488292 1105 66-67 -0.3555268878146336 1105 68-69 -0.023527981976044998 1105 70-71 -0.044789393186157156 1105 72-73 0.013484823388459688 1105 74-75 -0.09758650953336456 1105 76-77 0.03268257577962075 1105 78-79 0.008245877665874701 1105 80-81 0.07820595290651511 1105 82-83 0.03898286194589673 1105 84-85 -0.13583625032694258 1105 86-87 0.08587634972903757 1105 88-89 0.15829025344213576 1105 90-91 0.11005591692006433 1105 92-93 -0.06084943894312289 1105 94-95 -0.05668824938443251 1105 96-97 0.03793300164335989 1105 98-99 -0.21824366377209614 1105 100-101 0.15450545124786075 1106 1 -0.08627340502514258 1106 2 -0.12719491432768848 1106 3 -0.12498377212550338 1106 4 0.16796292207030206 1106 5 0.1880889518115012 1106 6 0.2063196561111127 1106 7 0.3854116933346745 1106 8 0.3605911626522982 1106 9 0.39045371175775045 1106 10-11 0.4036525134382529 1106 12-13 0.39523789485988914 1106 14-15 0.3203144011491261 1106 16-17 0.31198262051721315 1106 18-19 0.32296909283362396 1106 20-21 0.5040862772765067 1106 22-23 0.385694447198496 1106 24-25 0.20016272550763148 1106 26-27 0.3362551812581742 1106 28-29 0.14105607948557264 1106 30-31 0.0937556995206279 1106 32-33 0.06729450181418173 1106 34-35 0.17801916563259823 1106 36-37 0.2534796118687126 1106 38-39 0.2959701836479596 1106 40-41 0.06880706587720198 1106 42-43 0.24006882378812122 1106 44-45 -0.009923716557963758 1106 46-47 -0.057544144711805245 1106 48-49 0.12291359349573128 1106 50-51 0.10557962335351334 1106 52-53 0.0785692681209369 1106 54-55 0.26016290633280903 1106 56-57 0.19369826015795866 1106 58-59 0.2185441224242055 1106 60-61 0.13364521468655255 1106 62-63 0.21691162395692487 1106 64-65 0.1288512227995895 1106 66-67 0.01373637590767629 1106 68-69 0.15238830841481388 1106 70-71 0.28525681818678095 1106 72-73 0.43052306241767013 1106 74-75 0.2054974822826381 1106 76-77 0.03620751581066273 1106 78-79 0.4206929638800716 1106 80-81 0.3838386254054562 1106 82-83 0.4195934870340885 1106 84-85 0.5917327139738937 1106 86-87 0.4618508452842782 1106 88-89 0.5700953190552305 1106 90-91 0.5817285216893602 1106 92-93 0.4501098879935199 1106 94-95 0.528447386286512 1106 96-97 0.6707483678067554 1106 98-99 0.9229162064127507 1106 100-101 1.4074827335958204 1107 1 0.3603375427344133 1107 2 0.4642384360318452 1107 3 0.5563121953112251 1107 4 0.41881312942827265 1107 5 0.4044776414732425 1107 6 0.5995706637887821 1107 7 0.6712070772049472 1107 8 0.6701983073053199 1107 9 0.8270361825331136 1107 10-11 0.8666479825277591 1107 12-13 0.7751823303354399 1107 14-15 0.9195236752242195 1107 16-17 0.8972918313395226 1107 18-19 1.0155503223724054 1107 20-21 1.1514960229685727 1107 22-23 0.8224945975230966 1107 24-25 1.0453181315031514 1107 26-27 1.0460291253469478 1107 28-29 0.9585539423884768 1107 30-31 0.9717883263106941 1107 32-33 0.8494717075233424 1107 34-35 0.7514499541221795 1107 36-37 1.0855699442501674 1107 38-39 1.1290595923800169 1107 40-41 0.9212094144344221 1107 42-43 1.0269473122848467 1107 44-45 0.9816744483174276 1107 46-47 1.149558428009037 1107 48-49 1.0050328395437518 1107 50-51 1.005544953419225 1107 52-53 1.0140395221112826 1107 54-55 1.077123186762293 1107 56-57 1.1014587014982808 1107 58-59 1.218188821761764 1107 60-61 1.0695207231568702 1107 62-63 1.2749725160063932 1107 64-65 1.049726360751528 1107 66-67 0.9698143991391817 1107 68-69 1.1515821001146804 1107 70-71 1.2956941886707583 1107 72-73 1.3694844596954212 1107 74-75 1.1779411701050222 1107 76-77 1.2605742601979664 1107 78-79 1.1351364320379815 1107 80-81 1.1778393373300595 1107 82-83 1.268291975054435 1107 84-85 1.164374496802484 1107 86-87 1.4490709384961171 1107 88-89 1.615664779342275 1107 90-91 1.5977484793973353 1107 92-93 1.626240432205627 1107 94-95 1.8074649303318857 1107 96-97 1.667635682580645 1107 98-99 1.9503163715580847 1107 100-101 2.000512621196421 1108 1 0.2224844144565381 1108 2 0.10649588102530316 1108 3 0.08502516442498731 1108 4 0.15768588900506586 1108 5 0.28974221365243125 1108 6 0.3157923996321159 1108 7 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-2.315936421680334 2316 74-75 -2.6670641627614984 2316 76-77 -3.656288967566862 2316 78-79 -3.4709239962877483 2316 80-81 -3.580135599099645 2316 82-83 -3.3807720211484913 2316 84-85 -3.327759299494648 2316 86-87 -3.733319864068715 2316 88-89 -3.9486892038849106 2316 90-91 -3.954734687332799 2316 92-93 -4.131081205516779 2316 94-95 -4.257049670533284 2316 96-97 -4.665534476466906 2316 98-99 -4.8323372844783385 2316 100-101 -5.5244467281036265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2102.0 3 1001.0 4 1047.0 5 1081.0 6 1272.0 7 1424.0 8 1676.0 9 2052.0 10 2696.0 11 3010.0 12 3582.0 13 4132.0 14 4474.0 15 5175.0 16 5958.0 17 7083.0 18 6354.0 19 6132.0 20 6695.0 21 7615.0 22 8477.0 23 10231.0 24 11814.0 25 13647.0 26 16680.0 27 19762.0 28 24229.0 29 29366.0 30 35851.0 31 45178.0 32 57399.0 33 75077.0 34 101139.0 35 140174.0 36 196412.0 37 248202.0 38 169110.0 39 18701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.25708541860402 17.022600025466993 12.6819442890272 24.038370266901786 2 16.195819671941397 18.791799229431906 39.548523952352255 25.463857146274442 3 20.49151421474172 23.18915511010724 28.17066178552991 28.148668889621124 4 12.911806297432985 14.63212999956774 35.67392660199211 36.782137101007166 5 14.523684535573123 37.25435400197629 33.26171875 14.960242712450592 6 34.43892993032564 33.37142362998468 17.449859786529622 14.739786653160053 7 31.758280604313928 30.965763056703445 19.250041293035448 18.025915045947187 8 28.509571857819534 32.88762686001539 18.96604022318255 19.636761058982525 9 28.02825792689797 15.796224353245695 19.002483450097163 37.17303426975917 10-11 26.426023046111908 24.898725025755397 26.537150497603175 22.138101430529517 12-13 27.45450039987772 21.99721166346044 26.783470074015973 23.764817862645863 14-15 24.12251015464073 23.739477801025384 24.783690524058954 27.354321520274937 16-17 23.918137320731475 27.046914289154284 25.44918429058982 23.585764099524418 18-19 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26.32989158552267 24.599951311672044 44-45 23.92552945638247 25.180298547310503 26.31797437528719 24.57619762101984 46-47 24.35367435967377 25.342400482113458 26.057545735383137 24.24637942282963 48-49 24.247936537086005 25.34801284875552 25.991685191600933 24.412365422557546 50-51 23.91940008402898 25.57806573627787 26.380314842457242 24.122219337235908 52-53 24.2988873015181 25.404062597536175 26.054065857583392 24.24298424336233 54-55 23.66097763573098 25.28689822747147 26.33340882356005 24.7187153132375 56-57 24.050784793111923 25.562731171852004 25.838314447346157 24.54816958768992 58-59 24.343428458770383 25.013513195476232 25.93453247430178 24.708525871451606 60-61 24.097919003201802 25.193954117683827 26.621511979842428 24.086614899271947 62-63 23.781624521214702 25.337464544424794 26.56378762766322 24.317123306697283 64-65 23.741206763977257 25.541580002677566 26.269229567264567 24.44798366608061 66-67 23.78662876473021 25.535233677166865 25.8947696562282 24.783367901874726 68-69 23.759435918705933 25.31856896202483 26.437433082866317 24.48456203640292 70-71 23.860772802774974 25.197483539019228 26.39486675914011 24.546876899065683 72-73 23.79895049113018 25.13253898040765 26.319978671843863 24.748531856618307 74-75 23.679313746634264 25.83600862523626 25.98848193758394 24.496195690545537 76-77 23.84877381042404 25.355140489796803 26.191234955238023 24.60485074454114 78-79 23.834963390766468 25.252208306172143 26.361002434183078 24.55182586887831 80-81 23.90008412506078 25.643826841296914 26.11828446619176 24.337804567450547 82-83 23.86277616033446 25.497186986135944 26.28155911418807 24.358477739341527 84-85 23.688916720587365 25.327257174539863 26.113747019892593 24.870079084980183 86-87 23.76641716181804 25.378347094683228 26.250096458060035 24.60513928543869 88-89 23.834082343826683 25.23874709475497 25.948467975171535 24.978702586246808 90-91 23.659241847335384 24.965582011276137 26.43035296187629 24.94482317951219 92-93 23.67374248388156 25.12014792423359 26.554707386989566 24.65140220489528 94-95 23.36143830365483 24.946109526431314 26.759653696556608 24.932798473357252 96-97 23.190549606233592 24.99363399400901 26.881907116500226 24.933909283257172 98-99 23.073052174832497 25.34621271117567 26.334818544636995 25.245916569354844 100-101 23.030162672703447 25.02480908296134 26.724757168194195 25.220271076141014 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15.0 1 15.0 2 20.5 3 26.0 4 28.0 5 32.0 6 56.0 7 109.0 8 166.5 9 197.0 10 198.5 11 209.0 12 231.5 13 242.5 14 271.0 15 301.0 16 322.5 17 356.5 18 392.5 19 438.0 20 474.5 21 515.5 22 641.5 23 765.0 24 874.0 25 1097.5 26 1423.0 27 1904.0 28 2511.0 29 3497.5 30 4913.5 31 6815.5 32 9685.0 33 12273.0 34 14414.0 35 17345.5 36 21791.5 37 25799.0 38 28313.5 39 30222.5 40 32088.0 41 34895.0 42 37132.0 43 40321.5 44 44197.5 45 47996.0 46 51399.5 47 54891.0 48 63544.5 49 65069.0 50 58103.0 51 54723.0 52 53538.0 53 53593.5 54 53402.0 55 50503.5 56 45474.5 57 41658.0 58 40107.0 59 36653.0 60 30802.5 61 25661.5 62 21281.5 63 16515.5 64 12129.0 65 9366.5 66 7089.5 67 5505.5 68 4328.0 69 3246.5 70 2622.5 71 2074.0 72 1581.5 73 1436.0 74 1225.5 75 917.5 76 675.5 77 450.0 78 295.0 79 194.0 80 135.0 81 91.5 82 65.0 83 46.0 84 27.5 85 17.0 86 10.5 87 9.5 88 8.0 89 4.5 90 4.0 91 3.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01658937816837833 2 0.006404271571978612 3 0.010570906088687588 4 0.038425629431871666 5 0.050153933997422864 6 0.064968634501277 7 0.03024668019536886 8 0.03001520049999614 9 0.017901096442157084 10-11 0.05019251394665164 12-13 0.04737617765295021 14-15 0.022916489841899368 16-17 0.02530844669408415 18-19 0.03387319542287483 20-21 0.02650442512017654 22-23 0.015316239843828365 24-25 0.0035107753798196005 26-27 0.0030478159890741582 28-29 0.0015431979691514727 30-31 0.008140369287274017 32-33 0.0022376370552696354 34-35 0.002276217004498422 36-37 0.006944390861181627 38-39 0.007407350251927068 40-41 0.004320954313624123 42-43 8.873388322620968E-4 44-45 0.00266201649678629 46-47 0.0038194149736498944 48-49 6.944390861181627E-4 50-51 0.0021990571060408488 52-53 0.0012731383245499649 54-55 0.0028163362937014373 56-57 0.0024305368014135693 58-59 0.004205214465937763 60-61 0.0015817779183802593 62-63 0.0016975177660666196 64-65 0.00435953426285291 66-67 0.0026234365475575035 68-69 0.007523090099613429 70-71 0.010995285530204242 72-73 0.005439772841258941 74-75 0.0035107753798196005 76-77 0.003395035532133239 78-79 0.03885000887338832 80-81 0.024691167506423563 82-83 0.00532403299357258 84-85 0.00968356725642549 86-87 0.008487588830333098 88-89 0.006018472079690743 90-91 0.013580142128532957 92-93 0.016319318523776823 94-95 0.007291610404240709 96-97 0.004938233501284713 98-99 0.02677448476477805 100-101 0.04764623729755171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1296010.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.645436481762374 #Duplication Level Percentage of deduplicated Percentage of total 1 74.30518049804125 37.632182991794366 2 13.497013893566917 13.671243196802152 3 4.841571522550565 7.3561050905173255 4 2.2790102338616736 4.616858721613112 5 1.328021919589677 3.362912488748356 6 0.8136720810178846 2.472526661770481 7 0.5238504896445257 1.857144568946236 8 0.3888306914548949 1.57540000689909 9 0.2750206622006401 1.2535687330789271 >10 1.565206071969181 14.437501030772285 >50 0.12006260284349712 4.199677263707054 >100 0.05780606179468646 5.406061205406622 >500 0.0035266207642094165 1.1333018156453987 >1k 0.0012266507005945797 1.0255162242985567 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3334 0.2572511014575505 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2061 0.15902655072105926 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1707 0.1317119466670782 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1667 0.12862555072877524 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1324 0.10215970555782748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.857994922878682E-5 0.0 20-21 0.0 0.0 0.0 1.1573984768636046E-4 0.0 22-23 0.0 0.0 0.0 2.700596446015077E-4 0.0 24-25 0.0 0.0 0.0 4.2437944151665495E-4 0.0 26-27 0.0 0.0 0.0 6.558591368893759E-4 0.0 28-29 0.0 0.0 0.0 0.0011959784260923913 0.0 30-31 0.0 0.0 0.0 0.0021990571060408483 0.0 32-33 0.0 0.0 0.0 0.0034336154813620267 0.0 34-35 0.0 0.0 0.0 0.004706753805911991 0.0 36-37 0.0 0.0 0.0 0.006327111673521037 0.0 38-39 0.0 0.0 0.0 0.008564748728790674 0.0 40-41 0.0 0.0 0.0 0.013657302026990531 0.0 42-43 0.0 0.0 0.0 0.018865595172876753 0.0 44-45 0.0 0.0 0.0 0.03155839846914761 0.0 46-47 0.0 0.0 0.0 0.060570520289195295 0.0 48-49 0.0 0.0 0.0 0.08333269033417952 0.0 50-51 0.0 0.0 0.0 0.10906551646978033 0.0 52-53 0.0 0.0 0.0 0.1366116002191341 0.0 54-55 0.0 0.0 0.0 0.17214373345884676 0.0 56-57 0.0 0.0 0.0 0.21253694030138656 3.857994922878682E-5 58-59 0.0 0.0 0.0 0.27777563444726505 7.715989845757364E-5 60-61 0.0 0.0 0.0 0.3626515227505961 7.715989845757364E-5 62-63 0.0 0.0 0.0 0.46365382983155995 7.715989845757364E-5 64-65 0.0 0.0 0.0 0.5352196356509595 7.715989845757364E-5 66-67 0.0 0.0 0.0 0.6689763196271634 7.715989845757364E-5 68-69 0.0 0.0 0.0 0.8345614617171164 7.715989845757364E-5 70-71 0.0 0.0 0.0 0.9394603436701878 7.715989845757364E-5 72-73 0.0 0.0 0.0 1.131974290321834 7.715989845757364E-5 74-75 0.0 0.0 0.0 1.2806614146495783 7.715989845757364E-5 76-77 0.0 0.0 0.0 1.4038857724863234 7.715989845757364E-5 78-79 0.0 0.0 0.0 1.5679277166071248 7.715989845757364E-5 80-81 0.0 0.0 0.0 1.7212058548930949 7.715989845757364E-5 82-83 0.0 0.0 0.0 1.9347844538236587 7.715989845757364E-5 84-85 0.0 0.0 0.0 2.2867879105871096 7.715989845757364E-5 86-87 0.0 0.0 0.0 2.7356656198640446 7.715989845757364E-5 88-89 0.0 0.0 0.0 3.31424911844816 7.715989845757364E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 85 1.6275382E-5 33.543915 1 GTACATG 7755 0.0 32.905907 1 TACATGG 7840 0.0 32.00116 2 TAGATCG 75 3.07017E-4 31.681585 5 ACATGGG 7835 0.0 31.172527 3 CATGGGA 3935 0.0 27.170797 4 GTATAGG 305 0.0 26.486862 1 ATGGGAG 1960 0.0 26.185802 5 CATGGGG 3655 0.0 26.13204 4 ATGGGGG 1755 0.0 21.39184 5 ATGGGTA 585 0.0 21.121058 5 CATGGGT 1035 0.0 20.20119 4 TACCTGG 1140 0.0 20.007105 2 ATGGGGA 1980 0.0 19.440973 5 GAGTACT 1705 0.0 19.367926 12-13 ATGGGAT 1470 0.0 19.073608 5 AGTACAT 5300 0.0 18.917095 2 TCCGTTG 230 2.0505322E-6 18.594276 8 TAAGGCG 435 0.0 18.571964 5 ATGGGCG 260 3.264704E-7 18.277838 5 >>END_MODULE