FastQCFastQC Report
Thu 26 May 2016
SRR936554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296010
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44910.3465251039729632No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17010.13124898727633275No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14420.11126457357582117No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13750.10609486037916376No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13400.10339426393314866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81700.028.549271
TACATGG83100.027.775612
ACATGGG83550.026.716513
CATGGGG37300.023.555444
CATGGGA42700.022.2448984
ATGGGTA6700.021.9717665
GTATAAT6150.021.895671
GTATTAC1553.5507506E-521.7190911
GTATAGG3109.094947E-1221.7190911
GTGTACA11300.020.4285771
GAGTACT23100.019.01403612-13
CGTACAC1507.7295915E-418.9971453
CGATTCG2001.2878918E-518.9949469
ATGGGGG18600.018.6375145
ATGGGAT15750.018.3919325
TAGGACC4151.8189894E-1218.3105014
CTACACG3156.8193913E-918.092524
GTGCATA7450.018.0749471
GTACTTT23650.017.96951914-15
TAAGGTG4500.017.939675
Produced by FastQC (version 0.11.5)