##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936554_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1296010 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.650183254758836 31.0 31.0 34.0 30.0 34.0 2 31.909848689439126 33.0 31.0 34.0 30.0 34.0 3 32.22277991682163 34.0 31.0 34.0 30.0 34.0 4 35.98046542850749 37.0 35.0 37.0 35.0 37.0 5 35.82670967045007 37.0 35.0 37.0 35.0 37.0 6 35.92083703057847 37.0 35.0 37.0 35.0 37.0 7 35.86628961196287 37.0 35.0 37.0 35.0 37.0 8 35.83697656653884 37.0 35.0 37.0 35.0 37.0 9 37.52363253369958 39.0 37.0 39.0 35.0 39.0 10-11 37.40699840279011 39.0 37.0 39.0 34.5 39.0 12-13 37.29717633351595 39.0 37.0 39.0 34.0 39.0 14-15 38.50536724253671 40.0 38.0 41.0 34.0 41.0 16-17 38.42123131765959 40.0 38.0 41.0 34.0 41.0 18-19 38.41546824484379 40.0 38.0 41.0 33.5 41.0 20-21 38.45488576477033 40.0 38.0 41.0 34.0 41.0 22-23 38.36828573853597 40.0 38.0 41.0 34.0 41.0 24-25 38.25578236279041 40.0 38.0 41.0 34.0 41.0 26-27 38.06156433978133 40.0 37.5 41.0 33.0 41.0 28-29 38.00946983433769 40.0 37.5 41.0 33.0 41.0 30-31 38.093926744392405 40.0 38.0 41.0 33.0 41.0 32-33 38.007241842269735 40.0 37.0 41.0 33.0 41.0 34-35 37.885579586577265 40.0 37.0 41.0 33.0 41.0 36-37 37.691237336131664 40.0 37.0 41.0 33.0 41.0 38-39 37.555655820556936 40.0 37.0 41.0 33.0 41.0 40-41 37.357428569224005 39.5 36.5 41.0 32.0 41.0 42-43 37.17165801189806 39.0 36.0 41.0 31.0 41.0 44-45 36.98079027168001 39.0 36.0 41.0 31.0 41.0 46-47 36.697761977145234 39.0 35.5 40.5 31.0 41.0 48-49 36.31948634655597 39.0 35.0 40.0 30.0 41.0 50-51 36.166779191518586 39.0 35.0 40.0 30.0 41.0 52-53 35.90844360768821 38.0 35.0 40.0 29.0 41.0 54-55 35.63111820124845 38.0 34.5 40.0 28.5 41.0 56-57 35.33300476076573 38.0 34.0 40.0 28.0 41.0 58-59 34.94372034166403 37.5 34.0 40.0 27.5 41.0 60-61 34.54257798936736 37.0 33.5 40.0 26.0 41.0 62-63 34.2489942207236 36.0 33.0 39.0 26.0 40.5 64-65 33.80220291510096 36.0 33.0 39.0 26.0 40.0 66-67 33.382378608189754 35.0 32.0 38.5 26.0 40.0 68-69 32.90923719724385 35.0 32.0 38.0 25.0 40.0 70-71 32.44332644038241 35.0 31.0 37.0 24.5 39.0 72-73 32.03245461068973 35.0 31.0 36.5 24.0 39.0 74-75 31.662447049019683 34.0 31.0 36.0 23.0 39.0 76-77 30.227180731630156 33.0 29.5 35.0 21.0 36.5 78-79 31.052749592981534 34.0 30.5 35.0 22.5 37.0 80-81 30.92850170909175 34.0 31.0 35.0 21.5 37.0 82-83 30.63043687934507 34.0 30.5 35.0 20.0 36.0 84-85 30.225955046643158 34.0 30.0 35.0 19.5 36.0 86-87 29.872551909321686 34.0 30.0 35.0 18.0 35.5 88-89 29.506368006419706 34.0 29.0 35.0 16.0 35.0 90-91 29.09604941319897 33.5 29.0 35.0 8.5 35.0 92-93 28.685479278709266 33.0 29.0 35.0 4.5 35.0 94-95 28.19938619300777 33.0 28.5 35.0 2.0 35.0 96-97 27.549672456231047 33.0 27.0 34.5 2.0 35.0 98-99 26.741886250877695 33.0 25.5 34.0 2.0 35.0 100-101 23.967666530350847 29.5 19.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3493188033262342 1101 2 0.2947629678861752 1101 3 0.1912841237065166 1101 4 0.11819074799005591 1101 5 0.21650788630132212 1101 6 0.17791974433124125 1101 7 0.15160749430417297 1101 8 0.14097536011282585 1101 9 0.15611290954199575 1101 10-11 0.21614715023760311 1101 12-13 0.26220125614924683 1101 14-15 0.3143479892535552 1101 16-17 0.28727863582587787 1101 18-19 0.22733409763525714 1101 20-21 0.028123745937655542 1101 22-23 0.012618495985385891 1101 24-25 -0.08998871704403655 1101 26-27 -0.08141237164016957 1101 28-29 -0.028012121957580405 1101 30-31 -0.11140009093568892 1101 32-33 -0.03240827442133565 1101 34-35 0.0696291487980858 1101 36-37 0.01546598417193934 1101 38-39 -0.043119199194187274 1101 40-41 0.01183302585401691 1101 42-43 -0.006544875033647202 1101 44-45 0.07880597452232507 1101 46-47 0.13326882894466507 1101 48-49 -0.02207039743353789 1101 50-51 -0.11197875936079527 1101 52-53 -0.08554821801162404 1101 54-55 -0.13274920106800892 1101 56-57 -0.19133053359940533 1101 58-59 -0.1740879672534632 1101 60-61 0.09676582631843189 1101 62-63 -0.06139798460037582 1101 64-65 -0.035737545343202726 1101 66-67 -0.1948283853095205 1101 68-69 -0.3598975599707117 1101 70-71 -0.32536597052816063 1101 72-73 -0.08484531508529614 1101 74-75 -0.12952786195143062 1101 76-77 0.13460807540704067 1101 78-79 0.009619233707265806 1101 80-81 -0.10732172753236213 1101 82-83 -0.2720445910553799 1101 84-85 -0.07516206781943424 1101 86-87 -0.2501647066631527 1101 88-89 -0.22034258492856296 1101 90-91 -0.29061109650543315 1101 92-93 -0.3054722343965999 1101 94-95 -0.449519026859452 1101 96-97 -0.4895777305864577 1101 98-99 -0.5769348711123321 1101 100-101 -0.4687316985706609 1102 1 0.2779531516461944 1102 2 0.4370061982551654 1102 3 0.18489447144020232 1102 4 0.12706766712090456 1102 5 0.17717257429369937 1102 6 0.03081410510952054 1102 7 0.16246675193489324 1102 8 -0.03425456916789216 1102 9 -0.036520559367168914 1102 10-11 -0.014421901830843353 1102 12-13 0.08015442752442681 1102 14-15 0.2556465858604895 1102 16-17 0.28749628999758414 1102 18-19 0.1457581202067999 1102 20-21 0.18752611301837163 1102 22-23 0.16350853299392298 1102 24-25 0.19050924931849522 1102 26-27 -0.13634769830463966 1102 28-29 -0.21989772527002316 1102 30-31 0.002648862931884821 1102 32-33 0.20468673858660935 1102 34-35 0.34091455424309913 1102 36-37 0.30551215323866643 1102 38-39 0.1277103783222131 1102 40-41 0.09655289687376722 1102 42-43 0.21015586880131565 1102 44-45 0.02282524827557353 1102 46-47 0.10655013047264106 1102 48-49 -0.051186956005956574 1102 50-51 -0.09393251700983996 1102 52-53 -0.2851993850630308 1102 54-55 -0.1042592226868635 1102 56-57 -0.12598041817673789 1102 58-59 -0.3321360417102426 1102 60-61 -0.18530225473392647 1102 62-63 -0.32514097679560905 1102 64-65 -0.38516482906310756 1102 66-67 -0.1809941542443383 1102 68-69 -0.26625350148989213 1102 70-71 -0.5828387637567012 1102 72-73 -0.6130861270745989 1102 74-75 -0.8787030479101716 1102 76-77 -0.5421879379306915 1102 78-79 -0.6065927042942754 1102 80-81 -0.3484147618660245 1102 82-83 -0.2934289302148123 1102 84-85 -0.28744267314719707 1102 86-87 -0.5289148440710925 1102 88-89 -0.5012707297382057 1102 90-91 -0.36330539132790207 1102 92-93 -0.251367950933183 1102 94-95 -0.22709918148918362 1102 96-97 -0.3634137937574664 1102 98-99 -0.7013341236131545 1102 100-101 -0.928279534853683 1103 1 0.2705919828829977 1103 2 0.32315962098432394 1103 3 0.19841664292859207 1103 4 0.10222964441701521 1103 5 -0.026255569148702307 1103 6 -0.033968541512265915 1103 7 0.006960275850985909 1103 8 0.012379702039552853 1103 9 -0.15205534011879251 1103 10-11 -0.041133492005727135 1103 12-13 -0.1034036737467332 1103 14-15 -0.06409276153794252 1103 16-17 -0.01917658805471234 1103 18-19 0.06718484390390955 1103 20-21 -0.05495517762634705 1103 22-23 -0.06760990745197404 1103 24-25 -0.02603236245335694 1103 26-27 -0.05649830560670921 1103 28-29 -0.026316056828676437 1103 30-31 -0.02554075127038402 1103 32-33 -0.1727248732454072 1103 34-35 -0.18731341112955135 1103 36-37 -0.13377644007047707 1103 38-39 -0.05247695586538015 1103 40-41 0.011472329336598364 1103 42-43 0.10107837236572692 1103 44-45 -0.035331293682105525 1103 46-47 -0.14034831262023317 1103 48-49 0.011615717446609608 1103 50-51 -0.2195092885331178 1103 52-53 -0.050198828595064526 1103 54-55 -0.15686950862115623 1103 56-57 -0.2679384033416028 1103 58-59 -0.3195662576198117 1103 60-61 -0.22712946992761118 1103 62-63 -0.2419486404122324 1103 64-65 -0.25557359238073474 1103 66-67 -0.39197334234253844 1103 68-69 -0.35294256223213694 1103 70-71 -0.5465848760008001 1103 72-73 -0.4626698288799602 1103 74-75 -0.21632795240823555 1103 76-77 -0.42638921088875037 1103 78-79 -0.36864399560073835 1103 80-81 -0.42211480758663456 1103 82-83 -0.26372256482290624 1103 84-85 -0.2956674952278462 1103 86-87 -0.5211201566857682 1103 88-89 -0.6179613094875869 1103 90-91 -0.564565189675946 1103 92-93 -0.3702291317281201 1103 94-95 -0.5230671951091139 1103 96-97 -0.8413122398582829 1103 98-99 -0.7613616010987592 1103 100-101 -0.517570459312406 1104 1 0.14761152435091418 1104 2 0.28179748834853413 1104 3 -0.05844018119820049 1104 4 -0.006950614084296092 1104 5 -0.05186527833473065 1104 6 -0.00786516663408321 1104 7 -0.04043483621753552 1104 8 -0.027940603648502815 1104 9 -0.07514607202626422 1104 10-11 -0.15186206563244298 1104 12-13 -0.11435567918346834 1104 14-15 -8.859220241674848E-4 1104 16-17 -0.09050486932876112 1104 18-19 -0.07327586800048635 1104 20-21 -0.19559540432788936 1104 22-23 -0.18745687108740583 1104 24-25 0.011255425411448527 1104 26-27 0.03562644435483264 1104 28-29 -0.051832802994759675 1104 30-31 -0.03173240896131091 1104 32-33 0.0038022847789065395 1104 34-35 -0.03478353473830964 1104 36-37 0.009801738623679057 1104 38-39 0.15128996981550102 1104 40-41 0.1746208265413003 1104 42-43 -0.004846577626587134 1104 44-45 0.05609783644047894 1104 46-47 0.0113112375669715 1104 48-49 0.15247232359114804 1104 50-51 0.03409863832742843 1104 52-53 -0.06688569067642192 1104 54-55 0.09792129252774373 1104 56-57 0.15793961635373677 1104 58-59 0.3502883995069226 1104 60-61 -0.03806352388463097 1104 62-63 -0.3639706181523934 1104 64-65 0.01506259523787179 1104 66-67 0.020793685918221172 1104 68-69 0.027835570301071755 1104 70-71 -0.11994071279620044 1104 72-73 -0.11486952626930247 1104 74-75 -0.06875076104546096 1104 76-77 -0.1880047799506599 1104 78-79 0.034591117493580015 1104 80-81 0.10824723529175628 1104 82-83 0.15550539332449986 1104 84-85 0.26020969100593305 1104 86-87 0.36584161948492167 1104 88-89 0.15259634040726766 1104 90-91 -0.058142727245826364 1104 92-93 0.20904588494110854 1104 94-95 0.22533961106619316 1104 96-97 -0.11106047035528022 1104 98-99 -0.07852578020323975 1104 100-101 -0.2224321046937483 1105 1 0.41396393889242233 1105 2 0.43339135704088605 1105 3 0.19653133263793876 1105 4 0.062481019122436976 1105 5 -0.023643628850194887 1105 6 0.034606347202057464 1105 7 0.11756841934404605 1105 8 0.05905749727390486 1105 9 -0.10901069492371818 1105 10-11 -0.0198795642759535 1105 12-13 -0.039162380214392556 1105 14-15 0.11467294938114492 1105 16-17 0.13056810951008657 1105 18-19 0.29468058886332216 1105 20-21 0.2824499022374951 1105 22-23 0.15104353585778796 1105 24-25 0.006446689654524107 1105 26-27 -0.08269626318722345 1105 28-29 0.007883013608612544 1105 30-31 0.02053970172778463 1105 32-33 0.08236644647180213 1105 34-35 -0.09365833126571488 1105 36-37 0.022254261683904986 1105 38-39 0.0347137354150604 1105 40-41 0.006328639889105148 1105 42-43 -0.011642562433969772 1105 44-45 -0.09586906535007955 1105 46-47 0.04627122128916028 1105 48-49 0.13494315918528343 1105 50-51 0.029418768549895447 1105 52-53 0.039944206230806856 1105 54-55 -0.021879982567213574 1105 56-57 0.03423166735753114 1105 58-59 0.18816482381511435 1105 60-61 -0.053846215148873 1105 62-63 -0.08458578364344049 1105 64-65 -0.05911673194606948 1105 66-67 -0.0075975240655026255 1105 68-69 -0.27853990446832455 1105 70-71 -0.3265501810544791 1105 72-73 -0.25913159897684324 1105 74-75 -0.29866462431187557 1105 76-77 -0.21231975553394733 1105 78-79 -0.19829344571857277 1105 80-81 -0.33679142450206 1105 82-83 -0.16710041242699347 1105 84-85 0.10305762915026406 1105 86-87 -0.1093991564239225 1105 88-89 0.030672167862505262 1105 90-91 -0.28226779507002675 1105 92-93 -0.28236418017012355 1105 94-95 0.09542913900976302 1105 96-97 -0.20565827285120974 1105 98-99 -0.22293367494008365 1105 100-101 0.17600115629377555 1106 1 0.24913925990272645 1106 2 0.19544864503297887 1106 3 -0.012499286806118448 1106 4 -7.474273288181621E-4 1106 5 -0.2054800899761986 1106 6 -0.09329156446720077 1106 7 -0.002861593355262926 1106 8 0.03795724328773531 1106 9 -0.017933723003295654 1106 10-11 -0.19821754226381927 1106 12-13 -0.11369321131359555 1106 14-15 0.05706835646454067 1106 16-17 0.01668882349343903 1106 18-19 -0.04789825267751979 1106 20-21 -0.0449223991594323 1106 22-23 -0.14830562457680685 1106 24-25 -0.15032627492851702 1106 26-27 -0.22243804391249 1106 28-29 -0.07478683964382782 1106 30-31 -0.20651423505788102 1106 32-33 -0.13638178973533854 1106 34-35 -0.22302460049105832 1106 36-37 -0.3125074499006857 1106 38-39 -0.057903909568707945 1106 40-41 0.10281604640807984 1106 42-43 -0.24897764780153153 1106 44-45 -0.011583577837733117 1106 46-47 0.0026473324786593366 1106 48-49 -0.18542688455678302 1106 50-51 -0.26580928986692953 1106 52-53 -0.10980445328662114 1106 54-55 -0.23920748362549205 1106 56-57 -0.41872268568324955 1106 58-59 -0.333937264515626 1106 60-61 -0.14394869151404777 1106 62-63 -0.5648357130917248 1106 64-65 -0.3593751276000816 1106 66-67 -0.2270548050024317 1106 68-69 -0.39478179102116684 1106 70-71 -0.23574130564880846 1106 72-73 0.09079608551589047 1106 74-75 -0.13129567412138599 1106 76-77 -0.0248329839804029 1106 78-79 -0.06512973046529069 1106 80-81 -0.045605097424310514 1106 82-83 -0.0420827744983896 1106 84-85 -0.17390282661381207 1106 86-87 -0.08037065353130046 1106 88-89 -0.1994789891856108 1106 90-91 -0.28334811819687644 1106 92-93 -0.1216888238839644 1106 94-95 -0.13675478930969476 1106 96-97 -0.23131264000233287 1106 98-99 -0.3139480322139647 1106 100-101 -0.2112660230486867 1107 1 0.5923365424150475 1107 2 0.6306013118390332 1107 3 0.3954116776267682 1107 4 0.2046408362367913 1107 5 0.06019151428899505 1107 6 0.16785243750184264 1107 7 0.245563041882626 1107 8 0.25941144853904063 1107 9 0.31856048500042533 1107 10-11 0.4136032512277552 1107 12-13 0.33159280241147115 1107 14-15 0.4006464127780376 1107 16-17 0.5063506990045568 1107 18-19 0.34277996798734733 1107 20-21 0.43224142818958455 1107 22-23 0.5426181968439181 1107 24-25 0.37753439163405744 1107 26-27 0.5377264000849777 1107 28-29 0.4326956467462395 1107 30-31 0.5116873481142363 1107 32-33 0.6120064553043463 1107 34-35 0.5105816152194507 1107 36-37 0.6799369524928593 1107 38-39 0.52864753029219 1107 40-41 0.5608985079204842 1107 42-43 0.5397358991444676 1107 44-45 0.5987174286489818 1107 46-47 0.5335075438329397 1107 48-49 0.5075811027609021 1107 50-51 0.5470822355837086 1107 52-53 0.14768729606298336 1107 54-55 0.1969684093899815 1107 56-57 0.2977487089744102 1107 58-59 0.18342317576607314 1107 60-61 0.12350967148281455 1107 62-63 0.3545914257917957 1107 64-65 0.2665544765539565 1107 66-67 0.3831039488913177 1107 68-69 0.4381792595572378 1107 70-71 0.23715130489138403 1107 72-73 0.49547626198850026 1107 74-75 0.7629888151851958 1107 76-77 0.7449057211637182 1107 78-79 0.7333767496365446 1107 80-81 0.4903186445996184 1107 82-83 0.7290652237760504 1107 84-85 0.7495963754483874 1107 86-87 0.9512388650859265 1107 88-89 0.9267036922559164 1107 90-91 0.6362308670591368 1107 92-93 0.8487318997384357 1107 94-95 0.8095865401631919 1107 96-97 0.37294163882333464 1107 98-99 0.5957708634295358 1107 100-101 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-1.1860932526227366 2316 94-95 -1.6141963300559503 2316 96-97 -1.5209726671550712 2316 98-99 -1.7705961641154886 2316 100-101 -2.7856376871585518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 12.0 5 47.0 6 80.0 7 215.0 8 434.0 9 723.0 10 1120.0 11 1732.0 12 2306.0 13 3018.0 14 3870.0 15 4868.0 16 6059.0 17 7147.0 18 6448.0 19 5484.0 20 5733.0 21 6296.0 22 6812.0 23 8063.0 24 9391.0 25 11580.0 26 14328.0 27 18001.0 28 22733.0 29 28817.0 30 35768.0 31 45937.0 32 58510.0 33 77254.0 34 105412.0 35 147607.0 36 214755.0 37 274799.0 38 153583.0 39 7056.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69028156931255 17.791730278506567 12.915794852402302 23.602193299778577 2 16.550090827292326 19.646167119131146 38.41022142721547 25.39352062636106 3 20.974481288258584 23.377504242058535 28.263028463267514 27.384986006415367 4 13.597384797747814 15.183885266953128 35.30793142193905 35.91079851336001 5 14.681213879522534 37.81436871629077 32.776367466300414 14.728049937886281 6 34.94857292767803 33.73816560057407 16.830580010956705 14.482681460791197 7 32.11086334210384 31.256857585975418 18.846768157653106 17.78551091426764 8 28.87801791652842 33.15344789006257 18.50695596484595 19.461578228563052 9 28.31403347811761 15.938179761426166 18.616907583697223 37.130879176758995 10-11 26.65859571637786 25.189505295500176 26.099107565860034 22.052791422261933 12-13 27.710326958362845 22.126517932787348 26.537377141772488 23.625777967077312 14-15 24.212331858806536 23.97938594562229 24.547283236459005 27.260998959112165 16-17 24.22022206618776 27.17660357558969 25.096758512665794 23.506415845556745 18-19 24.03712162714794 26.196209905787764 26.80662186248563 22.960046604578668 20-21 25.184373577364372 25.103278524085464 26.108209041596904 23.604138856953263 22-23 24.561087418315154 25.06965733452573 26.181628365099343 24.187626882059774 24-25 24.64575890618128 25.23275283369727 26.5556978727016 23.56579038741985 26-27 24.402868960451293 25.43222098294804 26.61230492523396 23.552605131366708 28-29 23.909769214743715 25.863573583537164 26.56742617726715 23.65923102445197 30-31 24.042021280699995 25.421678845070637 26.48575242474981 24.050547449479556 32-33 24.14512329924591 25.471504109974862 26.331001035933582 24.052371554845642 34-35 24.592429412751905 25.281085491733236 26.444161951848088 23.682323143666775 36-37 24.748034178537214 25.25892977365419 26.231413867485827 23.761622180322764 38-39 24.494525505204436 25.246525875571947 26.58231803766946 23.676630581554154 40-41 24.38875895207222 25.387810597648482 26.465878917749354 23.75755153252995 42-43 24.27378103058367 25.521814639710115 26.117760816644974 24.086643513061233 44-45 24.376301140914432 25.31011328896015 26.093537081647987 24.220048488477428 46-47 24.792880186483064 25.7416536714432 25.721205967025956 23.74426017504778 48-49 24.867847562838964 25.56387612165587 25.62553439744662 23.942741918058545 50-51 24.409489829459613 25.839661789601525 26.058257805953406 23.692590574985456 52-53 24.747486049038248 25.71101292624509 25.706035951504973 23.835465073211683 54-55 24.090373288523534 25.63743033034366 25.942018640310682 24.330177740822126 56-57 24.58258549300361 25.80369364962028 25.60345506090494 24.010265796471174 58-59 24.970688419929328 25.187588325368377 25.53157259375944 24.310150660942856 60-61 24.41239815017019 25.497433064048774 26.389629470724273 23.70053931505676 62-63 24.238391841610195 25.512098535273953 26.28940955690918 23.960100066206667 64-65 24.174298049154697 25.846655131484102 25.89010500514761 24.088941814213587 66-67 24.271791470722928 25.793777100808157 25.670471920100095 24.263959508368817 68-69 24.24520200091203 25.595154045998136 26.006841001875756 24.152802951214074 70-71 24.390652289570227 25.55288536658857 25.9528864468384 24.10357589700281 72-73 24.33063788088055 25.459139975771794 25.95581824214319 24.254403901204466 74-75 24.20374842786707 26.223370190044832 25.504818635658676 24.068062746429426 76-77 24.414252237473505 25.689722089548322 25.766535776041998 24.12948989693617 78-79 24.336933328703115 25.598436529342084 25.77245559812785 24.292174543826953 80-81 24.52152888499252 25.94772008513699 25.547309217360166 23.98344181251032 82-83 24.350946132146344 25.867118989897197 25.677896419163492 24.104038458792964 84-85 24.18211331170848 25.70605196215252 25.704740117857632 24.407094608281366 86-87 24.23572454096785 25.73547200431531 25.592978825120007 24.435824629596834 88-89 24.21822930756819 25.82736730959891 25.366422751457723 24.58798063137518 90-91 24.172627944122407 25.42805618940558 25.83059436801583 24.568721498456185 92-93 24.08146305404275 25.537047811144358 25.943151637301366 24.438337497511526 94-95 23.796611111196853 25.535233917903017 26.06105339838041 24.60710157251972 96-97 23.773281907866306 25.42180612660666 26.190602968231037 24.614308997295996 98-99 23.627902382483825 25.871674537283727 25.610302399152374 24.890120681080074 100-101 23.300888459137283 26.057210810910924 25.67957902177663 24.962321708175168 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 35.0 1 26.5 2 27.0 3 39.0 4 38.5 5 40.0 6 64.0 7 124.0 8 200.0 9 242.5 10 234.5 11 225.5 12 259.5 13 277.5 14 289.0 15 320.5 16 356.5 17 388.5 18 431.5 19 482.0 20 535.0 21 614.5 22 716.5 23 814.5 24 940.0 25 1178.5 26 1478.5 27 1899.0 28 2576.5 29 3643.5 30 5057.5 31 6986.0 32 9768.5 33 12086.5 34 14169.5 35 17301.5 36 21729.5 37 25517.0 38 28170.5 39 30347.0 40 32077.5 41 34708.0 42 36679.5 43 39776.0 44 43739.0 45 46847.5 46 49691.0 47 53835.0 48 63968.0 49 64907.0 50 57330.0 51 54624.5 52 53553.5 53 53719.5 54 53153.5 55 50354.5 56 45545.5 57 41858.5 58 41012.5 59 37949.0 60 31555.0 61 25869.5 62 21269.0 63 16698.5 64 12480.0 65 9463.5 66 7100.0 67 5644.0 68 4423.0 69 3222.5 70 2621.5 71 2149.0 72 1748.5 73 1559.0 74 1314.5 75 1102.5 76 854.0 77 577.5 78 417.5 79 296.5 80 223.0 81 178.5 82 122.0 83 76.0 84 49.0 85 22.0 86 11.0 87 8.0 88 4.5 89 3.0 90 1.0 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.2432002839484262 2 0.011111025377890603 3 0.004552434008996844 4 0.015586299488429874 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004552434008996844 10-11 3.0863959383029455E-4 12-13 3.0863959383029455E-4 14-15 7.715989845757364E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0017746776645241936 24-25 0.0 26-27 3.857994922878682E-5 28-29 0.0 30-31 0.0 32-33 0.005979892130461956 34-35 5.786992384318022E-4 36-37 0.0038965748721074686 38-39 0.0 40-41 0.006249951775063464 42-43 0.013232922585473879 44-45 0.0024691167506423564 46-47 0.0016203578676090462 48-49 0.012191263956296633 50-51 9.259187814908835E-4 52-53 0.0032407157352180925 54-55 0.012538483499355716 56-57 0.004320954313624123 58-59 0.0015817779183802593 60-61 0.008256109134960379 62-63 0.005246873095115007 64-65 0.020370213192799438 66-67 0.002970656090616585 68-69 7.715989845757364E-5 70-71 0.001041658629177244 72-73 0.0 74-75 0.0 76-77 0.0013117182737787518 78-79 0.013502982230075386 80-81 0.016666538066835903 82-83 0.014004521570049614 84-85 0.009606407357967917 86-87 0.01222984390552542 88-89 0.0315969784183764 90-91 0.027507503800125 92-93 0.0027777563444726506 94-95 0.009876467002569425 96-97 0.01188262436246634 98-99 0.012075524108610272 100-101 0.01319434263624509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1296010.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.33825622796037 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58520463825579 35.08642220162946 2 14.15996720477544 13.689362458479017 3 5.2287706278528026 7.58248963099146 4 2.5303767361556333 4.892559961022443 5 1.4044006773489777 3.3943139894208 6 0.8688199509725381 2.5198344843644707 7 0.6004895591835849 2.031863272182177 8 0.40561087872382173 1.5685218067680202 9 0.32115729900053935 1.3971765427710976 >10 1.6934879561634566 15.046710188285072 >50 0.12986488060653337 4.3196141370713885 >100 0.06606305643708295 5.865468074270134 >500 0.004179163822789494 1.3579968491014633 >1k 0.0016073707010728821 1.2476664036429395 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4491 0.3465251039729632 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1701 0.13124898727633275 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1442 0.11126457357582117 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1375 0.10609486037916376 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1340 0.10339426393314866 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.715989845757364E-5 0.0 5 0.0 0.0 0.0 7.715989845757364E-5 0.0 6 0.0 0.0 0.0 7.715989845757364E-5 0.0 7 0.0 0.0 0.0 7.715989845757364E-5 0.0 8 0.0 0.0 0.0 7.715989845757364E-5 0.0 9 0.0 0.0 0.0 7.715989845757364E-5 0.0 10-11 0.0 0.0 0.0 7.715989845757364E-5 0.0 12-13 0.0 0.0 0.0 7.715989845757364E-5 0.0 14-15 0.0 0.0 0.0 7.715989845757364E-5 0.0 16-17 0.0 0.0 0.0 7.715989845757364E-5 0.0 18-19 0.0 0.0 0.0 7.715989845757364E-5 0.0 20-21 0.0 0.0 0.0 1.1573984768636046E-4 0.0 22-23 0.0 0.0 0.0 2.700596446015077E-4 0.0 24-25 0.0 0.0 0.0 4.2437944151665495E-4 0.0 26-27 0.0 0.0 0.0 6.558591368893759E-4 0.0 28-29 0.0 0.0 0.0 0.0011959784260923913 0.0 30-31 0.0 0.0 0.0 0.002276217004498422 0.0 32-33 0.0 0.0 0.0 0.0035107753798196 0.0 34-35 0.0 0.0 0.0 0.004783913704369565 0.0 36-37 0.0 0.0 0.0 0.006404271571978612 0.0 38-39 0.0 0.0 0.0 0.008834808373392181 0.0 40-41 0.0 0.0 0.0 0.0140431015192784 0.0 42-43 0.0 0.0 0.0 0.01952145430976613 0.0 44-45 0.0 0.0 0.0 0.0324843172506385 0.0 46-47 0.0 0.0 0.0 0.06165075886760133 0.0 48-49 0.0 0.0 0.0 0.08418144921721282 0.0 50-51 0.0 0.0 0.0 0.10991427535281365 0.0 52-53 0.0 0.0 0.0 0.13734461925448105 0.0 54-55 0.0 0.0 0.0 0.17318539208802403 0.0 56-57 0.0 0.0 0.0 0.21346285908287743 0.0 58-59 0.0 0.0 0.0 0.27808427404109537 0.0 60-61 0.0 0.0 0.0 0.36249720295368093 0.0 62-63 0.0 0.0 0.0 0.4638853095269327 0.0 64-65 0.0 0.0 0.0 0.5349495760063581 0.0 66-67 0.0 0.0 0.0 0.6682433005918165 0.0 68-69 0.0 0.0 0.0 0.8341756622248285 0.0 70-71 0.0 0.0 0.0 0.9380328855487226 0.0 72-73 0.0 0.0 0.0 1.1304310923526826 0.0 74-75 0.0 0.0 0.0 1.2801984552588328 0.0 76-77 0.0 0.0 0.0 1.403615712841722 0.0 78-79 0.0 0.0 0.0 1.5678119767594385 0.0 80-81 0.0 0.0 0.0 1.721553074436154 0.0 82-83 0.0 0.0 0.0 1.935671792655921 0.0 84-85 0.0 0.0 0.0 2.288562588251634 0.0 86-87 0.0 0.0 0.0 2.737324557680882 0.0 88-89 0.0 0.0 0.0 3.3155608367219385 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8170 0.0 28.54927 1 TACATGG 8310 0.0 27.77561 2 ACATGGG 8355 0.0 26.71651 3 CATGGGG 3730 0.0 23.55544 4 CATGGGA 4270 0.0 22.244898 4 ATGGGTA 670 0.0 21.971766 5 GTATAAT 615 0.0 21.89567 1 GTATTAC 155 3.5507506E-5 21.719091 1 GTATAGG 310 9.094947E-12 21.719091 1 GTGTACA 1130 0.0 20.428577 1 GAGTACT 2310 0.0 19.014036 12-13 CGTACAC 150 7.7295915E-4 18.997145 3 CGATTCG 200 1.2878918E-5 18.994946 9 ATGGGGG 1860 0.0 18.637514 5 ATGGGAT 1575 0.0 18.391932 5 TAGGACC 415 1.8189894E-12 18.310501 4 CTACACG 315 6.8193913E-9 18.09252 4 GTGCATA 745 0.0 18.074947 1 GTACTTT 2365 0.0 17.969519 14-15 TAAGGTG 450 0.0 17.93967 5 >>END_MODULE