FastQCFastQC Report
Thu 26 May 2016
SRR936553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363027
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33560.24621669269941096No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21170.15531607224214927No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17420.12780377791489092No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16350.11995360326684651No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA14560.1068210681079685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC1901.2732926E-1130.0198063
GTACATG80500.029.5816271
TACATGG82200.028.6807232
ACATGGG80950.027.8905433
CATGGGA40600.023.765684
TATTCCG2202.1555024E-923.7639335
GTGTAAG4650.023.5101341
CCGTTGC1652.417306E-623.044669
GTATAGG3251.8189894E-1221.9375511
CATGGGG37550.021.7720224
TAAGGTG4400.021.6035775
TCCGTTG1856.516255E-620.5541028
ATGGGAG18800.020.2246255
GTGTAGC8200.019.7081241
GAGTACT18400.019.24220712-13
GTGTAGG5450.019.186971
CATGGGC16450.019.0703324
ATGGGGG17100.018.0661495
GTACTTT19350.017.92909214-15
GTAAGGT4253.6379788E-1217.8941574
Produced by FastQC (version 0.11.5)