##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936553_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1363027 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.556231094468416 31.0 31.0 34.0 30.0 34.0 2 31.609465549838706 33.0 31.0 34.0 30.0 34.0 3 31.68007677030609 33.0 31.0 34.0 30.0 34.0 4 35.292416070994925 37.0 35.0 37.0 33.0 37.0 5 35.261463639384985 37.0 35.0 37.0 33.0 37.0 6 35.382203727439 37.0 35.0 37.0 33.0 37.0 7 35.23894977869111 37.0 35.0 37.0 32.0 37.0 8 35.179743321298844 37.0 35.0 37.0 32.0 37.0 9 36.6949297409369 39.0 37.0 39.0 32.0 39.0 10-11 36.592468454403324 39.0 37.0 39.0 32.0 39.0 12-13 36.509109870897646 39.0 37.0 39.0 32.0 39.0 14-15 37.61620019265943 40.0 37.0 41.0 32.0 41.0 16-17 37.49315200652665 40.0 37.0 41.0 32.0 41.0 18-19 37.40442999294952 40.0 37.0 41.0 32.0 41.0 20-21 37.283616538777295 39.5 37.0 41.0 31.5 41.0 22-23 37.14165640152396 39.0 36.0 41.0 31.0 41.0 24-25 37.28533330594331 39.0 37.0 41.0 31.0 41.0 26-27 37.279743541397195 39.0 37.0 41.0 31.0 41.0 28-29 37.20022273953487 39.0 37.0 41.0 31.0 41.0 30-31 37.05447177495384 39.0 36.0 41.0 31.0 41.0 32-33 36.935256234836146 39.0 36.0 41.0 30.5 41.0 34-35 36.73855433531397 39.0 36.0 41.0 30.0 41.0 36-37 36.57461370904612 39.0 35.5 40.5 30.0 41.0 38-39 36.37858604415027 39.0 35.0 40.0 30.0 41.0 40-41 36.2240146380079 39.0 35.0 40.0 30.0 41.0 42-43 36.02859591189316 38.0 35.0 40.0 29.0 41.0 44-45 35.8406579620213 38.0 35.0 40.0 28.5 41.0 46-47 35.682286557786455 38.0 35.0 40.0 28.0 41.0 48-49 35.70003822374758 38.0 35.0 40.0 28.0 41.0 50-51 34.78545582736072 37.5 34.0 39.5 27.0 40.5 52-53 34.95068733047842 38.0 34.0 39.5 27.5 40.5 54-55 35.36709801053097 38.0 34.0 40.0 28.0 41.0 56-57 35.256652656183626 38.0 34.0 40.0 27.5 41.0 58-59 34.98491005680738 38.0 34.0 40.0 26.5 41.0 60-61 34.63812272244057 37.0 34.0 40.0 26.0 41.0 62-63 34.29865439202598 37.0 33.5 40.0 26.0 41.0 64-65 33.935456157508256 36.0 33.0 39.0 26.0 41.0 66-67 33.52566530230142 36.0 33.0 39.0 25.5 40.5 68-69 33.104835414118725 35.0 32.0 38.5 24.5 40.0 70-71 32.64377851649307 35.0 32.0 37.5 23.5 40.0 72-73 32.256415683621825 35.0 31.5 37.0 22.5 39.0 74-75 31.825299132005455 35.0 31.0 36.5 22.0 39.0 76-77 31.40005260350675 35.0 31.0 36.0 20.5 38.5 78-79 30.965563778267047 34.0 31.0 35.5 20.0 37.0 80-81 30.514391864577885 34.0 30.0 35.0 18.0 37.0 82-83 30.06359265076921 34.0 30.0 35.0 16.0 36.5 84-85 29.761718586645753 34.0 29.5 35.0 13.5 36.0 86-87 29.4505937886777 34.0 29.0 35.0 8.5 36.0 88-89 29.069878659777096 34.0 29.0 35.0 6.0 35.0 90-91 28.674387961500393 34.0 29.0 35.0 2.0 35.0 92-93 28.328713957977357 34.0 29.0 35.0 2.0 35.0 94-95 27.868114498098716 33.0 27.0 35.0 2.0 35.0 96-97 27.291191223651474 33.0 26.5 35.0 2.0 35.0 98-99 26.55503009111338 33.0 25.0 35.0 2.0 35.0 100-101 24.026179965620635 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3758888674396452 1101 2 0.44293596198194507 1101 3 0.37386276029055665 1101 4 0.49693117163728573 1101 5 0.43563657728403626 1101 6 0.32542459318512584 1101 7 0.41462365239888044 1101 8 0.5594311495100399 1101 9 0.6716979242491377 1101 10-11 0.48846907925061345 1101 12-13 0.7167377235580545 1101 14-15 0.7834191890942108 1101 16-17 0.694627887399875 1101 18-19 0.7542308127829003 1101 20-21 0.7474688879575311 1101 22-23 0.7734652328318319 1101 24-25 0.7826144210052277 1101 26-27 0.5173028661060002 1101 28-29 0.7126077004878155 1101 30-31 0.7230736863222518 1101 32-33 0.4453329700793418 1101 34-35 0.49822143428128385 1101 36-37 0.43284389949115365 1101 38-39 0.41191718568872204 1101 40-41 0.2953057529898473 1101 42-43 0.3085031214015075 1101 44-45 0.39126079407448344 1101 46-47 0.5930478337911609 1101 48-49 0.5107793997578653 1101 50-51 0.5553785493910581 1101 52-53 0.6147616448463253 1101 54-55 0.6419446432749609 1101 56-57 0.486280590360046 1101 58-59 0.5070038121788656 1101 60-61 0.4074974558803319 1101 62-63 0.4414775378408464 1101 64-65 0.42767202229779855 1101 66-67 0.3807690336565841 1101 68-69 0.4383324216206077 1101 70-71 0.45549575038615586 1101 72-73 0.4416905763396599 1101 74-75 0.1549249996586859 1101 76-77 0.4093915552318741 1101 78-79 0.3301502198283792 1101 80-81 0.41212883596266536 1101 82-83 0.38406560450579974 1101 84-85 0.26609059006808167 1101 86-87 0.055716009367973385 1101 88-89 0.15225412125147386 1101 90-91 -0.147286154996511 1101 92-93 -0.024305910986459622 1101 94-95 0.1317896489616679 1101 96-97 0.04046970361163105 1101 98-99 -0.0176373712673481 1101 100-101 0.5954644876431239 1102 1 0.2749167881146626 1102 2 0.3040708707656883 1102 3 0.24010393791504825 1102 4 0.21714326895064318 1102 5 0.23750960980589042 1102 6 0.2790835187135343 1102 7 0.21354021331046624 1102 8 0.2462319816723948 1102 9 0.372249585437892 1102 10-11 0.4144466965386613 1102 12-13 0.40083412815229735 1102 14-15 0.419557500722739 1102 16-17 0.43947424562200865 1102 18-19 0.6345756143703554 1102 20-21 0.5677281246794266 1102 22-23 0.4592433766934292 1102 24-25 0.24386135677686838 1102 26-27 0.35953680701392443 1102 28-29 0.45427756983192324 1102 30-31 0.2711295276722794 1102 32-33 0.3045737954093113 1102 34-35 0.14126668677904064 1102 36-37 0.18356850526157587 1102 38-39 0.18193125078047245 1102 40-41 0.26275287211476694 1102 42-43 0.25944177278072544 1102 44-45 0.09541163084068671 1102 46-47 0.0983889656440553 1102 48-49 0.21965218407221698 1102 50-51 0.1785346745783869 1102 52-53 0.2147360567880412 1102 54-55 0.4919162195126958 1102 56-57 0.3091304928785519 1102 58-59 0.36207064805365263 1102 60-61 0.2655739745165775 1102 62-63 0.45820135228306214 1102 64-65 0.3173539630669424 1102 66-67 0.29637447980956466 1102 68-69 0.14742138666205307 1102 70-71 -0.024359908945051245 1102 72-73 -0.1287251245195904 1102 74-75 -0.019097816749162888 1102 76-77 0.21406743093062275 1102 78-79 0.24739703816851133 1102 80-81 0.2831163148283302 1102 82-83 0.3825776939459047 1102 84-85 0.2208731215977764 1102 86-87 0.12302177698970596 1102 88-89 0.1758129831266686 1102 90-91 0.2166240739548897 1102 92-93 0.10804490095899055 1102 94-95 0.013553803795108621 1102 96-97 -0.016591499554216682 1102 98-99 -0.06762866398806366 1102 100-101 0.18440517539141155 1103 1 0.17196991303283937 1103 2 0.32825200343397753 1103 3 0.05418112483411974 1103 4 0.25395498837566777 1103 5 0.31428938620470603 1103 6 0.14431536748163865 1103 7 0.1491488326909831 1103 8 0.13728400691262266 1103 9 0.32362811029847194 1103 10-11 0.4220024004634695 1103 12-13 0.16107291536461332 1103 14-15 0.21499252808377634 1103 16-17 0.3204530899128457 1103 18-19 0.3277379739650499 1103 20-21 0.4481067280884261 1103 22-23 0.3889117714254482 1103 24-25 0.4406467624794814 1103 26-27 0.2460768798021391 1103 28-29 -0.22686818986225887 1103 30-31 0.030178306244273756 1103 32-33 0.2567954066993394 1103 34-35 0.2232588057537228 1103 36-37 0.2766392250269831 1103 38-39 0.16422512662159505 1103 40-41 0.06613176353466343 1103 42-43 0.10762337673956779 1103 44-45 0.1368171239938789 1103 46-47 0.1857760522829679 1103 48-49 0.02953684880135654 1103 50-51 0.06682179525494547 1103 52-53 0.19591127214163606 1103 54-55 0.19829173335030958 1103 56-57 0.21169117153694117 1103 58-59 0.0720508800333377 1103 60-61 0.1477369363158587 1103 62-63 0.15652107055603892 1103 64-65 0.11967048703730399 1103 66-67 0.05236772464500916 1103 68-69 0.015886812263595118 1103 70-71 0.01712282349283356 1103 72-73 0.21516642628814964 1103 74-75 -0.006396536409830844 1103 76-77 0.023616955712650878 1103 78-79 9.585546767745257E-4 1103 80-81 0.0033202789055160054 1103 82-83 -0.06548543280507246 1103 84-85 -0.030041967554687687 1103 86-87 0.018913694356761823 1103 88-89 0.23559553490317242 1103 90-91 0.2568408676081795 1103 92-93 0.21209996340498805 1103 94-95 -0.0027345212906375593 1103 96-97 -0.005355801913285774 1103 98-99 0.06348043977440554 1103 100-101 0.3673555851523673 1104 1 0.04677013843697253 1104 2 0.23879236231537604 1104 3 0.16694022077344783 1104 4 0.14140126818909948 1104 5 0.21032410658816758 1104 6 0.12103254639956162 1104 7 0.2846941138011658 1104 8 0.22342199226135762 1104 9 0.4003843275826213 1104 10-11 0.37320172696678355 1104 12-13 0.3139829709688229 1104 14-15 0.3547534435504289 1104 16-17 0.20396039857394044 1104 18-19 0.31825317856003466 1104 20-21 0.42578713626000564 1104 22-23 0.34169130310084483 1104 24-25 0.33730185342189856 1104 26-27 -0.15833004254885452 1104 28-29 0.25089606593069647 1104 30-31 0.20895908714738454 1104 32-33 -0.09838939753173293 1104 34-35 -0.03779872661417727 1104 36-37 -0.033449646662390364 1104 38-39 0.03854874449369561 1104 40-41 0.13108549458407737 1104 42-43 0.034459028612396025 1104 44-45 0.1602695073710052 1104 46-47 0.11876228362093144 1104 48-49 -0.0751256899824142 1104 50-51 -0.0526734556429318 1104 52-53 0.18276348986030655 1104 54-55 0.19838712274952996 1104 56-57 -0.07226711512008421 1104 58-59 -0.007298333428444437 1104 60-61 0.028410059588836134 1104 62-63 0.3117640928724086 1104 64-65 0.15309072316850347 1104 66-67 0.06981583756432741 1104 68-69 0.11660257222177961 1104 70-71 0.05546647653341097 1104 72-73 0.059047756444094546 1104 74-75 -0.11174716883136426 1104 76-77 0.06545250574625427 1104 78-79 0.12106484087212621 1104 80-81 0.02904656224937341 1104 82-83 0.23700843986874887 1104 84-85 0.14300424708164527 1104 86-87 0.23132331205234635 1104 88-89 0.302432273060969 1104 90-91 0.28250435010201613 1104 92-93 0.24526492256746124 1104 94-95 0.35158074576573384 1104 96-97 0.325373842124705 1104 98-99 0.6388531713058612 1104 100-101 1.3165125261738808 1105 1 0.27689954153510854 1105 2 0.2851072454220649 1105 3 0.23781957042847068 1105 4 0.3514003073303087 1105 5 0.4254611332441769 1105 6 0.3446407722445741 1105 7 0.2736375341158066 1105 8 0.266218940454678 1105 9 0.37578708755637535 1105 10-11 0.44163684553601 1105 12-13 0.4179618574005275 1105 14-15 0.21998629538675374 1105 16-17 0.3735277663199099 1105 18-19 0.38612928587529183 1105 20-21 0.2896697580488521 1105 22-23 0.29014309145270545 1105 24-25 0.38754527098058844 1105 26-27 0.4263540173997953 1105 28-29 0.2125170198027675 1105 30-31 0.012799952545968551 1105 32-33 0.13871442512203203 1105 34-35 0.10809632079192255 1105 36-37 0.18184715069844515 1105 38-39 -0.005941996626361856 1105 40-41 -0.20282578165238618 1105 42-43 0.16029763859981472 1105 44-45 -0.040317212368833566 1105 46-47 -0.10542171264288669 1105 48-49 0.15190643323990827 1105 50-51 0.20018230604620868 1105 52-53 0.06091467838303544 1105 54-55 0.10674428513242873 1105 56-57 0.29106021967365336 1105 58-59 0.395927545105593 1105 60-61 0.024629936852164747 1105 62-63 0.09943608845692609 1105 64-65 0.21868702542636953 1105 66-67 0.19205551000831633 1105 68-69 0.15735490230432703 1105 70-71 0.3489295084227493 1105 72-73 0.26507692186003595 1105 74-75 0.42955597913567445 1105 76-77 0.021986308236893848 1105 78-79 0.28469592621798867 1105 80-81 0.14701655813034264 1105 82-83 0.3958156789341416 1105 84-85 0.510411135023908 1105 86-87 0.5265853471810438 1105 88-89 0.30501690870555365 1105 90-91 0.31026087891400067 1105 92-93 0.39818075655191976 1105 94-95 0.43355354526876866 1105 96-97 0.8800241751851736 1105 98-99 1.046970677631517 1105 100-101 1.5142338011515548 1106 1 0.17101654356373075 1106 2 0.08620379676808199 1106 3 0.282997485884362 1106 4 0.27670322963965077 1106 5 0.3041386923208762 1106 6 0.2015079250490075 1106 7 0.30866138410758026 1106 8 0.20180751438965672 1106 9 0.42254403695052645 1106 10-11 0.43178981444877707 1106 12-13 0.5065549820235873 1106 14-15 0.5744848972837886 1106 16-17 0.7174939925384578 1106 18-19 0.6451840586150297 1106 20-21 0.5335784153217347 1106 22-23 0.5197828743355402 1106 24-25 0.2810948076876443 1106 26-27 0.029846533436860057 1106 28-29 0.47344415396582207 1106 30-31 0.22536917234968 1106 32-33 0.21878460440818515 1106 34-35 0.3969690660082179 1106 36-37 0.2982899381319726 1106 38-39 0.3953903132590426 1106 40-41 0.2251061225090254 1106 42-43 0.05514384727353416 1106 44-45 0.3770960230059117 1106 46-47 0.36258091908033663 1106 48-49 0.36779272086532444 1106 50-51 0.13604775919605316 1106 52-53 0.39397570146544325 1106 54-55 0.503679424120314 1106 56-57 0.14487073560106012 1106 58-59 0.21047878146723775 1106 60-61 0.39954084205530194 1106 62-63 0.47515021752101916 1106 64-65 0.1709294729289823 1106 66-67 0.28883987318612725 1106 68-69 0.23307725550351677 1106 70-71 0.35139126619456107 1106 72-73 0.36115400941965703 1106 74-75 0.2493871102283869 1106 76-77 0.42056079148707326 1106 78-79 0.383848840303191 1106 80-81 0.48397033849110827 1106 82-83 0.785634199686374 1106 84-85 0.7134074615768995 1106 86-87 0.7761302783724453 1106 88-89 0.53765027676301 1106 90-91 0.8112751889090646 1106 92-93 0.874226592398184 1106 94-95 0.8428523006829884 1106 96-97 0.8476676863425858 1106 98-99 1.3449502817764838 1106 100-101 1.7250238663963593 1107 1 0.113909466174281 1107 2 0.11372872924150812 1107 3 0.02485705220290768 1107 4 0.10819576663082131 1107 5 0.2519358470285198 1107 6 0.2629120891337209 1107 7 0.35110931145740665 1107 8 0.3916531586827716 1107 9 0.19033855752805096 1107 10-11 0.31825211292164113 1107 12-13 0.27578596716666937 1107 14-15 0.22058994725669123 1107 16-17 0.35096795675315207 1107 18-19 0.4127477689384449 1107 20-21 0.5102218858097629 1107 22-23 0.5277336290570034 1107 24-25 0.6372469049518159 1107 26-27 0.26203452396262605 1107 28-29 0.39580921417297077 1107 30-31 0.35631307617370567 1107 32-33 0.2768615598719464 1107 34-35 0.3913240387924688 1107 36-37 0.2691564589858615 1107 38-39 0.39463332471751045 1107 40-41 0.3076208963084994 1107 42-43 0.3357026715014868 1107 44-45 0.43447838858431 1107 46-47 0.5131557855925521 1107 48-49 0.48642746417797866 1107 50-51 0.48688906149271105 1107 52-53 0.46978726184207886 1107 54-55 0.48313364099558953 1107 56-57 0.4388232478486884 1107 58-59 0.49083032988233555 1107 60-61 0.526163692918189 1107 62-63 0.424159072084656 1107 64-65 0.5011364680558401 1107 66-67 0.4301965797461875 1107 68-69 0.39886897353932227 1107 70-71 0.4761343845296082 1107 72-73 0.5141032635506022 1107 74-75 0.5019136080660296 1107 76-77 0.4499876954557571 1107 78-79 0.5184705943315748 1107 80-81 0.5063274099429655 1107 82-83 0.6891514111357715 1107 84-85 0.5435572399429205 1107 86-87 0.5759087523474555 1107 88-89 0.3604877530936257 1107 90-91 0.40728275447623474 1107 92-93 0.4468543099244364 1107 94-95 0.7335050251724091 1107 96-97 0.7767579830276823 1107 98-99 1.099409626568999 1107 100-101 1.59929294926566 1108 1 0.018332919748846876 1108 2 0.047468495391115084 1108 3 0.12453653616027793 1108 4 0.09864366299569838 1108 5 0.31336058520236065 1108 6 0.15837656640147202 1108 7 -0.03161210708287143 1108 8 0.09301245616125442 1108 9 0.17015998992399517 1108 10-11 0.004430279279645788 1108 12-13 0.3273541704716578 1108 14-15 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2316 84-85 -2.446103143393252 2316 86-87 -3.0684910331723643 2316 88-89 -3.58129049627793 2316 90-91 -3.406708808381481 2316 92-93 -3.9448284857102784 2316 94-95 -4.360538749653575 2316 96-97 -5.725906675454123 2316 98-99 -7.51226998832216 2316 100-101 -7.897765177251241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2373.0 3 1066.0 4 1122.0 5 1208.0 6 1301.0 7 1474.0 8 1764.0 9 2262.0 10 2605.0 11 3240.0 12 3835.0 13 4406.0 14 4824.0 15 5512.0 16 6156.0 17 7408.0 18 6930.0 19 6700.0 20 7209.0 21 8161.0 22 9187.0 23 11086.0 24 12972.0 25 15138.0 26 17819.0 27 21362.0 28 25311.0 29 31544.0 30 38285.0 31 48054.0 32 60974.0 33 79982.0 34 107367.0 35 148719.0 36 209013.0 37 258093.0 38 171165.0 39 17400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.26117398388304 17.01403261555624 12.603079542618934 24.121713857941785 2 16.31467827901785 18.772249239920832 39.397601943806606 25.515470537254703 3 20.488479999882586 23.150514743706875 28.14619616545475 28.21480909095579 4 12.974258199064936 14.646921033371774 35.57649463134396 36.80232613621933 5 14.543445784989888 37.30365617762247 33.17875665735585 14.974141380031785 6 34.59671422604615 33.28951029034051 17.373891302704184 14.739884180909154 7 31.874366425874857 30.945063205097807 19.160969851964786 18.01960051706255 8 28.536018578869175 32.88721146675592 18.958931538863542 19.61783841551136 9 27.96001758662477 15.752213558583058 18.952010387559003 37.33575846723316 10-11 26.425338868273563 24.82398669852561 26.587863505731306 22.162810927469526 12-13 27.474573459506914 21.96092651260023 26.78007804127556 23.784421986617303 14-15 24.111934070596075 23.690920687860707 24.766758145574038 27.430387095969177 16-17 23.994190700496258 26.9949280572503 25.406619767987703 23.604261474265737 18-19 23.89907305623508 25.96272038083423 26.866614425527935 23.27159213740276 20-21 24.97599717652687 25.013381780453685 26.105716615897222 23.90490442712222 22-23 24.356330729254573 24.90295244706597 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24.293122732286662 48-49 24.31082576184439 25.31397467953233 25.914007841480018 24.46119171714326 50-51 23.969668834768076 25.539197686315063 26.30815163821821 24.182981840698652 52-53 24.36123705168102 25.33459186267601 26.06127883553998 24.242892250102994 54-55 23.770180875822863 25.204633567242198 26.260048249205386 24.765137307729557 56-57 24.099742403546255 25.522081347059583 25.806534459992196 24.571641789401966 58-59 24.415854377519736 24.966862124793586 25.93181357088167 24.68546992680501 60-61 24.07692141188659 25.1908562179193 26.607167293468244 24.125055076725868 62-63 23.822657264873545 25.318843977118437 26.490619667973043 24.36787909003497 64-65 23.754993488747456 25.50932668146219 26.219695163331558 24.515984666458795 66-67 23.788186505991565 25.54563157230526 25.836441864355542 24.829740057347628 68-69 23.804343518990052 25.27752380085796 26.41675093156125 24.501381748590735 70-71 23.89076332212097 25.175825179640487 26.266929971851095 24.666481526387443 72-73 23.80548159680469 25.14433267277918 26.24173053939224 24.8084551910239 74-75 23.68876520483561 25.797944343643408 26.011191727483563 24.502098724037417 76-77 23.9085542638517 25.303418337792483 26.12180316135439 24.666224237001426 78-79 23.914053916236686 25.198806263630846 26.27012412437016 24.61701569576231 80-81 23.96129012849115 25.64286848982266 25.968728182314134 24.427113199372055 82-83 23.915000583303435 25.444392839821944 26.227413500502962 24.413193076371662 84-85 23.76605946220431 25.21944629527983 26.089306313419648 24.92518792909621 86-87 23.84879233433116 25.32920995480425 26.197870810588718 24.62412690027587 88-89 23.92224680980947 25.137351349447663 25.939007783243134 25.001394057499738 90-91 23.67195498952106 24.91853277929876 26.432907804748666 24.976604426431514 92-93 23.69568575308259 25.078928994540924 26.537789973476922 24.68759527889957 94-95 23.46275847545616 24.90162138523253 26.67155594134925 24.96406419796206 96-97 23.187382280279145 24.96509222769186 26.83796850339392 25.009556988635072 98-99 23.115582499014817 25.218043148510045 26.357004422872716 25.309369929602425 100-101 23.064391886213514 24.9714025468156 26.617448831935786 25.346756735035104 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 20.0 1 16.5 2 22.0 3 33.0 4 35.5 5 43.0 6 57.5 7 91.0 8 154.5 9 206.5 10 214.0 11 196.0 12 204.0 13 235.5 14 266.5 15 274.0 16 280.5 17 320.0 18 358.0 19 413.5 20 461.0 21 524.5 22 627.5 23 766.0 24 919.5 25 1086.5 26 1422.5 27 1986.0 28 2633.5 29 3598.5 30 5067.5 31 7156.5 32 10205.0 33 12673.0 34 14822.5 35 17934.5 36 22401.0 37 26595.0 38 29235.0 39 31395.5 40 33369.5 41 36162.5 42 38798.0 43 42195.5 44 46505.0 45 50735.0 46 54060.5 47 57635.5 48 66693.0 49 68150.5 50 61161.5 51 57723.5 52 56550.5 53 57038.0 54 56585.5 55 53379.0 56 48118.0 57 44117.0 58 42510.0 59 38663.0 60 32421.0 61 27216.5 62 22946.0 63 17882.5 64 13139.5 65 10164.0 66 7567.5 67 5872.0 68 4585.5 69 3451.5 70 2767.5 71 2155.0 72 1708.0 73 1530.0 74 1251.0 75 988.5 76 757.5 77 471.5 78 327.5 79 241.5 80 159.0 81 100.5 82 66.0 83 47.5 84 33.0 85 20.0 86 12.5 87 7.0 88 8.5 89 11.0 90 6.5 91 4.5 92 4.0 93 1.5 94 1.5 95 2.0 96 0.5 97 1.0 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035729299566332876 2 0.02494448019004759 3 0.022743496643866924 4 0.056345178782225155 5 0.05957328798329014 6 0.0535572662903963 7 0.052530140635511986 8 0.04541362716952782 9 0.046073922233382025 10-11 0.05663864325504924 12-13 0.04922866531624099 14-15 0.0440196709236134 16-17 0.05344721711308726 18-19 0.055354736186443855 20-21 0.012215458681302718 22-23 0.021496272634364544 24-25 0.006126070870202864 26-27 0.01159184667655153 28-29 0.007006464288675132 30-31 0.010564721021667216 32-33 0.004181868737743273 34-35 0.00884061724382569 36-37 0.010454671844358183 38-39 0.012655655390538851 40-41 0.0023110327234897032 42-43 0.002641180255416804 44-45 0.002861278610034871 46-47 5.502458865451675E-4 48-49 0.0013939562459144243 50-51 4.768797683391451E-4 52-53 0.004438650151464351 54-55 0.0075567101752203 56-57 0.017351086955724283 58-59 0.011775261972066584 60-61 0.011408431381036473 62-63 0.007006464288675132 64-65 1.4673223641204465E-4 66-67 0.00814363912086848 68-69 0.019148556851771827 70-71 0.007079830406881155 72-73 0.00220098354618067 74-75 0.0041085026195372505 76-77 0.007226562643293201 78-79 0.0058326063973787754 80-81 0.012508923154126808 82-83 0.0073366118206022336 84-85 0.02046914697948023 86-87 0.004915529919803496 88-89 0.007263245702396211 90-91 0.00407181956043424 92-93 0.018451578728814616 94-95 0.01320590127708402 96-97 0.01100491773090335 98-99 0.024797747953635547 100-101 0.042589031618595966 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1363027.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.84656364499391 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7271424604542 36.75044699018564 2 13.6814661735184 13.639481487502325 3 5.020184207554144 7.50716794834323 4 2.3492495593857865 4.6840807071959 5 1.3499796556748023 3.3645923413020493 6 0.825305037239405 2.468317203917284 7 0.5761588198895109 2.0103676099672962 8 0.3913995372807697 1.560793755654964 9 0.28908271236494676 1.2968801838510107 >10 1.5986784480678429 14.548758113275337 >50 0.12586943146626395 4.335245845336737 >100 0.06060594139744917 5.627149982263605 >500 0.003547647786572082 1.1307319722751075 >1k 0.0013303679199645306 1.0759858589295839 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3356 0.24621669269941096 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2117 0.15531607224214927 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1742 0.12780377791489092 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1635 0.11995360326684651 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1456 0.1068210681079685 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 7.336611820602234E-5 12-13 0.0 0.0 0.0 0.0 7.336611820602234E-5 14-15 0.0 0.0 0.0 0.0 7.336611820602234E-5 16-17 0.0 0.0 0.0 0.0 7.336611820602234E-5 18-19 0.0 0.0 0.0 0.0 7.336611820602234E-5 20-21 0.0 0.0 0.0 3.668305910301117E-5 7.336611820602234E-5 22-23 0.0 0.0 0.0 1.8341529551505582E-4 7.336611820602234E-5 24-25 0.0 0.0 0.0 2.9346447282408935E-4 7.336611820602234E-5 26-27 0.0 0.0 0.0 2.9346447282408935E-4 7.336611820602234E-5 28-29 0.0 0.0 0.0 8.070273002662457E-4 7.336611820602234E-5 30-31 0.0 0.0 0.0 0.0016874207187385136 7.336611820602234E-5 32-33 0.0 0.0 0.0 0.0028979616691378824 7.336611820602234E-5 34-35 0.0 0.0 0.0 0.0038517212058161725 7.336611820602234E-5 36-37 0.0 0.0 0.0 0.005575824983657697 7.336611820602234E-5 38-39 0.0 7.336611820602234E-5 0.0 0.0073366118206022336 7.336611820602234E-5 40-41 0.0 7.336611820602234E-5 0.0 0.011811945031169595 7.336611820602234E-5 42-43 0.0 7.336611820602234E-5 0.0 0.017791283664960415 7.336611820602234E-5 44-45 0.0 7.336611820602234E-5 0.0 0.03052030517370529 7.336611820602234E-5 46-47 0.0 7.336611820602234E-5 0.0 0.059903435515217235 7.336611820602234E-5 48-49 0.0 7.336611820602234E-5 0.0 0.0808494622630366 7.336611820602234E-5 50-51 0.0 7.336611820602234E-5 0.0 0.1080316090583679 7.336611820602234E-5 52-53 0.0 7.336611820602234E-5 0.0 0.141229777546593 7.336611820602234E-5 54-55 0.0 7.336611820602234E-5 0.0 0.17849976559525232 7.336611820602234E-5 56-57 0.0 7.336611820602234E-5 0.0 0.21991493932255193 7.336611820602234E-5 58-59 0.0 7.336611820602234E-5 0.0 0.2845138064029546 7.336611820602234E-5 60-61 0.0 7.336611820602234E-5 0.0 0.3681511811578201 7.336611820602234E-5 62-63 0.0 7.336611820602234E-5 0.0 0.4761827902161879 7.336611820602234E-5 64-65 0.0 7.336611820602234E-5 0.0 0.5543177061056017 7.336611820602234E-5 66-67 3.668305910301117E-5 7.336611820602234E-5 0.0 0.6878807242996654 7.336611820602234E-5 68-69 7.336611820602234E-5 7.336611820602234E-5 0.0 0.8539082497998939 7.336611820602234E-5 70-71 7.336611820602234E-5 7.336611820602234E-5 0.0 0.9629669845131461 7.336611820602234E-5 72-73 7.336611820602234E-5 7.336611820602234E-5 0.0 1.15914798459605 7.336611820602234E-5 74-75 7.336611820602234E-5 7.336611820602234E-5 0.0 1.3096952591548077 7.336611820602234E-5 76-77 7.336611820602234E-5 7.336611820602234E-5 0.0 1.435481468819033 7.336611820602234E-5 78-79 7.336611820602234E-5 7.336611820602234E-5 0.0 1.5997482074823168 7.336611820602234E-5 80-81 7.336611820602234E-5 7.336611820602234E-5 0.0 1.764088312263807 7.336611820602234E-5 82-83 7.336611820602234E-5 7.336611820602234E-5 0.0 1.9846268635911102 7.336611820602234E-5 84-85 7.336611820602234E-5 7.336611820602234E-5 0.0 2.3408560505404514 7.336611820602234E-5 86-87 7.336611820602234E-5 7.336611820602234E-5 0.0 2.795946081772408 7.336611820602234E-5 88-89 7.336611820602234E-5 7.336611820602234E-5 0.0 3.3559496620389764 7.336611820602234E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTACAC 190 1.2732926E-11 30.019806 3 GTACATG 8050 0.0 29.581627 1 TACATGG 8220 0.0 28.680723 2 ACATGGG 8095 0.0 27.890543 3 CATGGGA 4060 0.0 23.76568 4 TATTCCG 220 2.1555024E-9 23.763933 5 GTGTAAG 465 0.0 23.510134 1 CCGTTGC 165 2.417306E-6 23.04466 9 GTATAGG 325 1.8189894E-12 21.937551 1 CATGGGG 3755 0.0 21.772022 4 TAAGGTG 440 0.0 21.603577 5 TCCGTTG 185 6.516255E-6 20.554102 8 ATGGGAG 1880 0.0 20.224625 5 GTGTAGC 820 0.0 19.708124 1 GAGTACT 1840 0.0 19.242207 12-13 GTGTAGG 545 0.0 19.18697 1 CATGGGC 1645 0.0 19.070332 4 ATGGGGG 1710 0.0 18.066149 5 GTACTTT 1935 0.0 17.929092 14-15 GTAAGGT 425 3.6379788E-12 17.894157 4 >>END_MODULE