FastQCFastQC Report
Thu 26 May 2016
SRR936553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363027
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45740.3355766246743462No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17490.12831734074233306No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15480.11357075098292256No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14980.10990244507262145No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13960.10241910101560718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG83050.029.351791
TACATGG85950.028.187312
ACATGGG85300.027.116253
CGTATAG1501.0568856E-625.3428551
CATGGGG38050.023.716394
TAAGGTG5100.023.281965
CCTACAC5550.022.253
CATGGGA44150.021.7305324
CATGGGT12250.020.548944
GTAAGGT4700.020.2107224
GTATTAC1707.932297E-519.5661751
ATGGGAG19950.019.5218335
GTGCATA8400.019.2334181
GGGTACC6350.018.6988967
GTATAGG4350.018.5701961
ATACGTC2051.5911333E-518.534715
ATAGTAC3105.5915734E-918.3852373
ATGGGAT15050.018.3037955
TACCGGA1300.007560242418.2707332
GAGTACT21200.018.14741512-13
Produced by FastQC (version 0.11.5)