##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936553_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1363027 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06782917726501 31.0 31.0 34.0 31.0 34.0 2 32.213973017409046 33.0 31.0 34.0 30.0 34.0 3 32.339237593972825 34.0 31.0 34.0 31.0 34.0 4 36.040651432436775 37.0 35.0 37.0 35.0 37.0 5 35.845051492010064 37.0 35.0 37.0 35.0 37.0 6 35.941891833397285 37.0 35.0 37.0 35.0 37.0 7 35.88764859390166 37.0 35.0 37.0 35.0 37.0 8 35.854479038199536 37.0 35.0 37.0 35.0 37.0 9 37.5363481427734 39.0 37.0 39.0 35.0 39.0 10-11 37.404034182741796 39.0 37.0 39.0 34.0 39.0 12-13 37.32103839469064 39.0 37.0 39.0 34.0 39.0 14-15 38.49900809008186 40.0 38.0 41.0 34.0 41.0 16-17 38.420372450435686 40.0 38.0 41.0 34.0 41.0 18-19 38.510021444916354 40.0 38.0 41.0 34.0 41.0 20-21 38.50840922446878 40.0 38.0 41.0 34.0 41.0 22-23 38.413401568714335 40.0 38.0 41.0 34.0 41.0 24-25 38.28216095499209 40.0 38.0 41.0 34.0 41.0 26-27 38.07911948919573 40.0 37.5 41.0 33.0 41.0 28-29 38.02745433509388 40.0 37.5 41.0 33.0 41.0 30-31 38.09403335370466 40.0 38.0 41.0 33.0 41.0 32-33 38.02902033488698 40.0 37.0 41.0 33.0 41.0 34-35 37.8870539615136 40.0 37.0 41.0 33.0 41.0 36-37 37.66886936208894 40.0 37.0 41.0 33.0 41.0 38-39 37.52582230579438 40.0 37.0 41.0 32.0 41.0 40-41 37.33914661998625 39.5 36.5 41.0 32.0 41.0 42-43 37.141203732574624 39.0 36.0 41.0 31.0 41.0 44-45 36.9682199985767 39.0 36.0 41.0 31.0 41.0 46-47 36.72346585944372 39.0 35.5 40.5 31.0 41.0 48-49 36.45055637195742 39.0 35.0 40.0 30.0 41.0 50-51 36.1567698218744 38.5 35.0 40.0 30.0 41.0 52-53 35.88075584709621 38.0 35.0 40.0 29.0 41.0 54-55 35.60185344824424 38.0 34.0 40.0 28.5 41.0 56-57 35.265600387960035 38.0 34.0 40.0 28.0 41.0 58-59 34.91269321884306 37.0 34.0 40.0 27.5 41.0 60-61 34.52276037085105 37.0 33.0 39.5 26.5 41.0 62-63 34.115434617215946 36.0 33.0 39.0 26.0 40.0 64-65 33.68299674181069 36.0 32.5 39.0 26.0 40.0 66-67 33.20814004418108 35.0 32.0 38.5 25.5 40.0 68-69 32.90330418986565 35.0 32.0 38.0 25.0 40.0 70-71 32.46440312627703 35.0 31.0 37.0 24.5 39.0 72-73 32.02982039240602 35.0 31.0 36.5 23.5 39.0 74-75 31.51256651555692 34.0 31.0 36.0 22.0 38.5 76-77 30.078166096489653 32.5 29.5 35.0 20.5 36.5 78-79 30.88381374690303 34.0 30.0 35.0 21.5 37.0 80-81 30.71948721485341 34.0 30.0 35.0 20.0 37.0 82-83 30.42463282091991 34.0 30.0 35.0 20.0 36.0 84-85 30.04172881388263 34.0 30.0 35.0 18.5 36.0 86-87 29.691093059785317 34.0 29.0 35.0 17.5 35.0 88-89 29.32603536100165 34.0 29.0 35.0 12.5 35.0 90-91 28.874167569681305 33.0 29.0 35.0 7.0 35.0 92-93 28.433613934280096 33.0 29.0 35.0 2.0 35.0 94-95 27.876015295368326 33.0 27.0 34.5 2.0 35.0 96-97 27.145635046114272 33.0 26.5 34.0 2.0 35.0 98-99 26.224762238752426 32.0 25.0 34.0 2.0 35.0 100-101 23.025708955141756 29.0 10.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20153792383821667 1101 2 0.267637836210902 1101 3 0.20750782557362868 1101 4 0.1022494933057132 1101 5 0.13128101179453466 1101 6 0.036756317313475506 1101 7 0.13770952070024123 1101 8 0.12085119319400661 1101 9 0.1725802742797029 1101 10-11 0.25415289134237895 1101 12-13 0.08580819821595043 1101 14-15 0.24404719445313816 1101 16-17 0.3050384543455422 1101 18-19 0.2056584647704014 1101 20-21 0.17312754910581418 1101 22-23 0.22614111118363667 1101 24-25 0.09000670207011296 1101 26-27 0.09106740057529095 1101 28-29 -0.017609362693306707 1101 30-31 0.023198911137079392 1101 32-33 0.1445216582019242 1101 34-35 0.17609022156123189 1101 36-37 0.03299746196938713 1101 38-39 0.01708063586260522 1101 40-41 0.06317132943772918 1101 42-43 0.2326550360207662 1101 44-45 0.23003241170484756 1101 46-47 0.03931590801529694 1101 48-49 0.03563975868730296 1101 50-51 0.13174889561731362 1101 52-53 0.15143806842495877 1101 54-55 0.1257427216855831 1101 56-57 0.21252145071251505 1101 58-59 0.11091456538940037 1101 60-61 -0.035808612265462614 1101 62-63 0.12671784417037912 1101 64-65 0.15112589341468663 1101 66-67 0.18100782509014834 1101 68-69 -0.04341668572893553 1101 70-71 -0.12114310679235984 1101 72-73 0.04320179628271603 1101 74-75 -0.07033482413103442 1101 76-77 -0.06868000779183348 1101 78-79 -0.061307092283797715 1101 80-81 -0.01028515090056814 1101 82-83 0.10224841943670882 1101 84-85 0.2625255702570577 1101 86-87 0.1436305548950294 1101 88-89 0.07479933501604918 1101 90-91 -0.12496773069593203 1101 92-93 -0.17414649286947892 1101 94-95 -0.10423645233029433 1101 96-97 -0.08491277201237679 1101 98-99 0.0635732648783609 1101 100-101 0.18711657042199903 1102 1 0.22954100107745745 1102 2 0.22638067856043875 1102 3 0.2310975465803864 1102 4 0.06297232425905719 1102 5 0.058654963084478595 1102 6 0.12493236582336209 1102 7 0.1490173073432075 1102 8 0.16312853705346697 1102 9 0.1458549318934672 1102 10-11 0.22967259247919003 1102 12-13 0.23151884565053393 1102 14-15 0.2463624633512751 1102 16-17 0.35182323308723795 1102 18-19 0.19316868998534886 1102 20-21 0.04079158290474538 1102 22-23 0.08260077813186228 1102 24-25 0.1298491770458554 1102 26-27 -0.1394364673249413 1102 28-29 -0.07401201531089896 1102 30-31 0.12130025719572046 1102 32-33 0.1513693828997731 1102 34-35 0.19693481430743276 1102 36-37 0.3908577998785461 1102 38-39 0.3557145671625008 1102 40-41 0.11079447572135592 1102 42-43 0.19434033826677677 1102 44-45 0.12133520750879967 1102 46-47 0.19682575291229654 1102 48-49 0.1823233861999043 1102 50-51 0.049732830186606236 1102 52-53 -0.09057959167221696 1102 54-55 0.011898547956349148 1102 56-57 -0.06007510667612337 1102 58-59 -0.24775444857293394 1102 60-61 0.0401592301841589 1102 62-63 -0.018078205326993668 1102 64-65 0.02518410608047361 1102 66-67 -0.027937019466570234 1102 68-69 0.0020443196715760337 1102 70-71 0.021765031077570995 1102 72-73 -0.044410113940898555 1102 74-75 -0.024696503828593563 1102 76-77 -0.5211382628930963 1102 78-79 -0.6256678979173032 1102 80-81 0.028541975243552287 1102 82-83 -0.15577045100564746 1102 84-85 -0.22566172098509085 1102 86-87 -0.20198054264918497 1102 88-89 -0.5130446025157269 1102 90-91 -0.2844148618308928 1102 92-93 0.004934051799192929 1102 94-95 0.07990949368056732 1102 96-97 0.19741408657473514 1102 98-99 0.2140942671028867 1102 100-101 0.923788928513364 1103 1 0.10845467635826367 1103 2 0.10090349687793321 1103 3 0.11312566691657366 1103 4 0.0884442693798917 1103 5 -0.0924761342370175 1103 6 0.0380420979732321 1103 7 -0.04970235332759643 1103 8 -0.004144261626919388 1103 9 -0.03710398728019726 1103 10-11 -0.08801324000319966 1103 12-13 0.04305826386853795 1103 14-15 -0.02838225426713592 1103 16-17 0.1187563098290525 1103 18-19 -0.11226620646164776 1103 20-21 0.002354565650264817 1103 22-23 -0.11039859348073122 1103 24-25 -0.11888619330996164 1103 26-27 -0.07257015674446876 1103 28-29 -0.10843062605108855 1103 30-31 -0.07154332670708641 1103 32-33 -0.05587932759691938 1103 34-35 -0.1642053160829562 1103 36-37 -0.029812872757801756 1103 38-39 0.04194175539071665 1103 40-41 0.044371963742719345 1103 42-43 -0.09960816014916674 1103 44-45 0.0014048537790571913 1103 46-47 0.010279788992207273 1103 48-49 -0.12773670334529896 1103 50-51 0.023642316218079884 1103 52-53 -0.19923562811867868 1103 54-55 -0.25424838996421073 1103 56-57 -0.15449620478311488 1103 58-59 0.21566882270714416 1103 60-61 -0.12607738385506195 1103 62-63 -0.1778404654270176 1103 64-65 -0.3973736924689959 1103 66-67 -0.1778466887777057 1103 68-69 -0.1688403974231747 1103 70-71 -0.13483369120138633 1103 72-73 -0.31604562306560524 1103 74-75 -0.29345059489240555 1103 76-77 -0.18181103015411537 1103 78-79 -0.33359867057198045 1103 80-81 -0.24531383603076407 1103 82-83 -0.10344214477151681 1103 84-85 -0.2428049147640543 1103 86-87 -0.517505638871004 1103 88-89 -0.338980637914883 1103 90-91 -0.3366043991806684 1103 92-93 -0.3454770182921827 1103 94-95 -0.17312116638802877 1103 96-97 -0.045746963879540914 1103 98-99 0.054247567445884215 1103 100-101 0.4059846188231049 1104 1 0.08599098203885802 1104 2 0.13911662598968633 1104 3 0.12027070632835546 1104 4 0.07903975722368273 1104 5 -0.057558522162963754 1104 6 -0.013760456154308542 1104 7 0.09402435095168471 1104 8 0.04368656306705532 1104 9 0.04540614572181312 1104 10-11 0.047923443161415946 1104 12-13 -0.009604492172222479 1104 14-15 0.11054527694658134 1104 16-17 0.10469180486947494 1104 18-19 0.027181044218984596 1104 20-21 -0.0782635644459333 1104 22-23 0.03726368731035734 1104 24-25 0.01668573572847265 1104 26-27 0.10773515722583937 1104 28-29 0.1356761082368223 1104 30-31 0.07914086309406088 1104 32-33 0.07481874628403773 1104 34-35 0.07608125947155742 1104 36-37 0.1422451620168914 1104 38-39 0.0995393003866738 1104 40-41 0.07876052108838394 1104 42-43 0.07402379971261297 1104 44-45 0.17756802736061417 1104 46-47 0.21823122526461702 1104 48-49 0.08566482831651712 1104 50-51 -0.05852699487356716 1104 52-53 0.04258362764032597 1104 54-55 0.023415554200731492 1104 56-57 -0.06501976019382738 1104 58-59 -0.06775005556783498 1104 60-61 -0.10608108401325467 1104 62-63 -0.034068272964475455 1104 64-65 -0.07920846173528417 1104 66-67 -0.2240791469727057 1104 68-69 0.011628538221224005 1104 70-71 -0.13737746509116988 1104 72-73 -0.4431543737699961 1104 74-75 -0.1753286458255907 1104 76-77 -0.12204821368874974 1104 78-79 -0.18205343019748454 1104 80-81 -0.13849043881029743 1104 82-83 -0.10118063524276621 1104 84-85 -0.01871535735728358 1104 86-87 -0.20732833679551987 1104 88-89 -0.13906380660814932 1104 90-91 -0.02029484869897047 1104 92-93 -0.08414825414501692 1104 94-95 0.055056386890854014 1104 96-97 0.4237757836067324 1104 98-99 0.4453444436976568 1104 100-101 0.7000105606661222 1105 1 0.10898083194377506 1105 2 0.08506012600346935 1105 3 0.09600604261775203 1105 4 -0.025906877871200606 1105 5 -0.05478208718740518 1105 6 0.03389590499153883 1105 7 0.032006130387671305 1105 8 0.012068722675387278 1105 9 -0.07850069119810144 1105 10-11 -0.02042471396357115 1105 12-13 -0.10603002300945974 1105 14-15 0.04181929376086657 1105 16-17 0.04010365948334993 1105 18-19 0.00800781478750423 1105 20-21 0.06291210214053677 1105 22-23 0.046731684381597915 1105 24-25 0.01123621396054375 1105 26-27 0.03695282451395343 1105 28-29 0.027224386921616883 1105 30-31 0.07329694231351169 1105 32-33 0.08413386252932753 1105 34-35 0.16815760081924935 1105 36-37 0.027783045550378915 1105 38-39 0.1897704576852135 1105 40-41 0.10327638605868117 1105 42-43 -0.08344047161008206 1105 44-45 -0.05140275880808787 1105 46-47 0.04872139731793368 1105 48-49 0.024505980483027656 1105 50-51 0.14964715742924994 1105 52-53 0.13764961778295515 1105 54-55 0.1425627883054119 1105 56-57 0.010906891272895791 1105 58-59 0.06961643906289794 1105 60-61 0.05388123393566957 1105 62-63 0.05373466756135059 1105 64-65 -0.03198001793174399 1105 66-67 0.13539525582376655 1105 68-69 0.0857573037262469 1105 70-71 0.034182194928696674 1105 72-73 0.048875526069785735 1105 74-75 -0.13213897453612589 1105 76-77 -0.4414568535223786 1105 78-79 -0.17035273994334688 1105 80-81 -0.015339998863598936 1105 82-83 -0.07200181197448785 1105 84-85 -0.11725049462229364 1105 86-87 -0.10402670683638959 1105 88-89 -0.13437496471113164 1105 90-91 -0.23336919458876793 1105 92-93 -0.12091693074453147 1105 94-95 0.04686767273591386 1105 96-97 0.09412599056379278 1105 98-99 0.5528531826710577 1105 100-101 0.8519685152733665 1106 1 0.05649988452341148 1106 2 0.0877850560983191 1106 3 0.1517601958059771 1106 4 0.06869260674967137 1106 5 -0.03639840387143067 1106 6 -0.06956371450514354 1106 7 0.066552980543932 1106 8 0.11236479153880907 1106 9 0.11893337865221554 1106 10-11 0.027776941828960844 1106 12-13 0.0869959104298701 1106 14-15 0.13444498595890053 1106 16-17 0.1576128431008783 1106 18-19 -0.0201132444811023 1106 20-21 -0.013317406056188474 1106 22-23 0.06741538414583914 1106 24-25 -0.0723950158217832 1106 26-27 -0.05736429813531174 1106 28-29 -0.06631418427197389 1106 30-31 -0.019397284343654064 1106 32-33 -0.08111094255866647 1106 34-35 0.0034281748638775866 1106 36-37 -0.12606859105250123 1106 38-39 0.17029296440158959 1106 40-41 0.13953111493636072 1106 42-43 0.06427047745511771 1106 44-45 0.10264077428034568 1106 46-47 -0.048951172450145464 1106 48-49 0.13854457909040718 1106 50-51 0.17589311389615858 1106 52-53 0.16464397054352986 1106 54-55 0.22766065551304848 1106 56-57 0.08562315861210834 1106 58-59 0.1292331943674654 1106 60-61 0.16180655878900296 1106 62-63 0.2580742695132088 1106 64-65 0.11057713980716954 1106 66-67 0.2954753040720277 1106 68-69 0.31686098265601714 1106 70-71 0.2881846073416554 1106 72-73 0.16001922912368371 1106 74-75 0.12603581574843048 1106 76-77 -0.0926027496789672 1106 78-79 -0.0634472078418078 1106 80-81 0.06172014697773065 1106 82-83 0.15241838650130646 1106 84-85 -0.14081264166982876 1106 86-87 -0.08908619474691193 1106 88-89 0.008439882594093007 1106 90-91 0.20559949887030626 1106 92-93 0.10047404463789178 1106 94-95 0.3505054818263247 1106 96-97 0.09389571478113012 1106 98-99 0.5048434233640791 1106 100-101 0.64086045013822 1107 1 0.011825037659214388 1107 2 0.08583148156215259 1107 3 0.05803784607554263 1107 4 0.0533485828784066 1107 5 -0.05268294174353372 1107 6 -0.010321696243281053 1107 7 -0.014993642479666391 1107 8 -0.07167281174824325 1107 9 -0.012438463360432195 1107 10-11 0.10206675227954776 1107 12-13 0.08915956763885902 1107 14-15 0.041429749027535934 1107 16-17 0.08537938144365853 1107 18-19 -0.02899242211430675 1107 20-21 0.059200843931400016 1107 22-23 -0.013025983788161 1107 24-25 0.07769836625015358 1107 26-27 0.03395797041209647 1107 28-29 -0.04431120900041208 1107 30-31 -0.1513313619986718 1107 32-33 -0.04011869596953943 1107 34-35 0.0025774803819516023 1107 36-37 0.10512253236080937 1107 38-39 0.09239180473660014 1107 40-41 0.035965539130629054 1107 42-43 -0.0661047133573831 1107 44-45 -0.14038666169625458 1107 46-47 0.07347499093933152 1107 48-49 -0.013082643996661147 1107 50-51 -0.02861535094575629 1107 52-53 -0.1684415674343711 1107 54-55 -0.0035067175467133893 1107 56-57 -0.10559201743198798 1107 58-59 0.021100209660538383 1107 60-61 0.0951300321270736 1107 62-63 0.15561567609551474 1107 64-65 0.09103837167323547 1107 66-67 0.05847095775732214 1107 68-69 0.12868822690249004 1107 70-71 -0.010361655108390266 1107 72-73 0.23519859827111134 1107 74-75 0.10105605752702829 1107 76-77 -0.11053534617557403 1107 78-79 0.020454370693052226 1107 80-81 0.3136498632041871 1107 82-83 0.17404351523901695 1107 84-85 0.2064866192623498 1107 86-87 0.27046824862037155 1107 88-89 0.2361427268068894 1107 90-91 0.01569296730716374 1107 92-93 0.22362113921395732 1107 94-95 0.28937595819364503 1107 96-97 0.1566318418757504 1107 98-99 0.570005972942937 1107 100-101 0.9533742955147773 1108 1 -0.033050705006573367 1108 2 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-0.49889376191384116 2316 78-79 -0.15109802702812658 2316 80-81 -0.9307230180025847 2316 82-83 -0.5288313489036334 2316 84-85 -0.4441655903172226 2316 86-87 -0.49567355345314823 2316 88-89 -0.19149661459061562 2316 90-91 -0.4810945445656216 2316 92-93 -0.5964185509338442 2316 94-95 -0.6848952209381665 2316 96-97 -0.7273099920052033 2316 98-99 -0.9537273399397641 2316 100-101 -1.3256058298668592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 13.0 5 55.0 6 111.0 7 226.0 8 422.0 9 727.0 10 1122.0 11 1773.0 12 2474.0 13 3145.0 14 4055.0 15 5124.0 16 6305.0 17 7711.0 18 7199.0 19 5881.0 20 6203.0 21 6772.0 22 7596.0 23 8516.0 24 10482.0 25 12857.0 26 15515.0 27 19638.0 28 24378.0 29 30515.0 30 38783.0 31 48879.0 32 62782.0 33 82629.0 34 112709.0 35 157669.0 36 227184.0 37 286969.0 38 150641.0 39 5960.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.739304408582896 17.77546670737543 12.892910510200187 23.59231837384149 2 16.747337921946617 19.632940285662087 38.22151981856855 25.398201973822747 3 20.933387430840423 23.336519999325002 28.20133370409202 27.52875886574256 4 13.616587944071007 15.244524267542372 35.238156027744395 35.90073176064222 5 14.79043532871221 37.67982180693403 32.737253267753275 14.79248959660048 6 34.95927443790507 33.72752977566092 16.77341444697313 14.539781339460875 7 32.137074320611404 31.31324617927598 18.73704629475425 17.81263320535837 8 28.865459011450252 33.09134742011713 18.55297070417534 19.490222864257277 9 28.264668723890352 15.971143109107691 18.52799922523958 37.23618894176238 10-11 26.72968594784237 25.08103317481303 26.11788364384434 22.071397233500267 12-13 27.67727931862644 22.106489930569182 26.546694580346536 23.669536170457842 14-15 24.262402608383997 23.95293580764929 24.424802979039825 27.35985860492689 16-17 24.227270426187076 27.273067433214994 25.042791855864266 23.45687028473367 18-19 24.05865767882808 26.181542992178436 26.69598621303907 23.063813115954414 20-21 25.243227023382513 25.077529645414216 26.068669219318473 23.610574111884798 22-23 24.647331642701786 25.03687633505906 26.1211011139753 24.19469090826386 24-25 24.690960394361607 25.147339020244118 26.595601069102052 23.566099516292212 26-27 24.44613847794514 25.418328651711153 26.4966486384165 23.638884231927207 28-29 24.028432663372072 25.815635983842277 26.46771922011212 23.688212132673534 30-31 24.08901038474053 25.45969048638326 26.353741481625768 24.097557647250444 32-33 24.191750319602207 25.483328411673323 26.242706925234177 24.082214343490293 34-35 24.66610585045284 25.295538282677054 26.294992018824644 23.743363848045465 36-37 24.811360592111466 25.253631815351856 26.240753302909376 23.6942542896273 38-39 24.51877994555687 25.214434033561034 26.50811877701062 23.75866724387148 40-41 24.428686720469553 25.389838591342627 26.400807043286868 23.780667644900955 42-43 24.28764801690364 25.4872635764699 26.118765682088302 24.106322724538156 44-45 24.440586157930436 25.220953748372633 26.09031545221739 24.248144641479545 46-47 24.89133556831067 25.729360360932084 25.575218090037133 23.80408598072011 48-49 24.817225317072346 25.619049505618786 25.57822096129395 23.985504216014913 50-51 24.441266032019467 25.81285819043011 25.999650040406756 23.74622573714366 52-53 24.848577558994315 25.664723514416632 25.667474815506917 23.81922411108213 54-55 24.127812493350973 25.57566833286744 25.939429823306416 24.35708935047517 56-57 24.631926089915133 25.838485848390413 25.49853907627067 24.031048985423777 58-59 24.992342324331084 25.182802933064032 25.479315471659632 24.345539270945256 60-61 24.509424486533224 25.523279936285988 26.250297604744947 23.716997972435838 62-63 24.269875777081086 25.521312913944943 26.255931055582987 23.952880253390983 64-65 24.234582227082775 25.795078800747962 25.84721030420805 24.12312866796121 66-67 24.29453474675225 25.782740583433327 25.633509136390863 24.28921553342355 68-69 24.264012387548913 25.547155262195414 25.997711634383887 24.191120715871786 70-71 24.362722620641588 25.548247464279818 25.970249995414612 24.118779919663982 72-73 24.330972985802333 25.422567994039643 25.90543471761486 24.341024302543165 74-75 24.227827910379098 26.191302484847107 25.432392157841022 24.148477446932773 76-77 24.44892396693305 25.664264476877165 25.712760708514537 24.174050847675243 78-79 24.39900071901275 25.54729343057125 25.700230377555062 24.353475472860936 80-81 24.51219239295636 25.895834838940672 25.56922962917854 24.022743138924422 82-83 24.482437942628227 25.831641234745888 25.585227658337324 24.100693164288558 84-85 24.228732926764845 25.690009626164382 25.59818687672053 24.48307057035024 86-87 24.268071144423885 25.639153791635056 25.594068558901345 24.498706505039713 88-89 24.267119918027344 25.70604379579182 25.36390407614388 24.662932210036956 90-91 24.207192000067504 25.427492081917347 25.754157704212787 24.611158213802355 92-93 24.06316752512178 25.554461979566558 25.88685120180659 24.495519293505076 94-95 23.80086464158827 25.482385266243213 26.046328207968863 24.670421884199655 96-97 23.773338087524067 25.456527034324083 26.125415258017558 24.644719620134293 98-99 23.692126640527785 25.760651691592518 25.586363956518674 24.960857711361022 100-101 23.40475485605091 25.90481997146661 25.628877347869295 25.06154782461319 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 29.0 1 29.5 2 35.0 3 42.0 4 44.5 5 47.0 6 72.0 7 127.5 8 192.0 9 252.5 10 243.5 11 197.5 12 222.0 13 274.5 14 314.5 15 346.0 16 364.5 17 383.0 18 426.0 19 503.5 20 563.0 21 617.5 22 685.5 23 805.0 24 970.5 25 1197.0 26 1513.5 27 1983.5 28 2660.0 29 3695.0 30 5222.5 31 7259.5 32 10079.5 33 12510.5 34 14756.5 35 18086.0 36 22652.5 37 26498.0 38 29217.5 39 31418.5 40 33342.5 41 36119.5 42 38309.5 43 41719.5 44 45708.0 45 49015.0 46 52229.0 47 56586.5 48 66825.5 49 68176.5 50 60990.0 51 57824.0 52 56273.0 53 56546.5 54 56117.0 55 53250.5 56 47791.0 57 43606.0 58 43072.5 59 40432.5 60 33791.5 61 27617.0 62 22704.5 63 17814.0 64 13531.5 65 10326.0 66 7720.0 67 6203.5 68 4889.0 69 3535.0 70 2869.0 71 2329.0 72 1870.5 73 1714.5 74 1448.0 75 1217.5 76 922.5 77 595.5 78 414.0 79 305.0 80 241.0 81 178.0 82 129.0 83 83.5 84 46.5 85 27.5 86 18.0 87 10.0 88 7.0 89 4.5 90 2.5 91 2.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03690315745762923 2 0.006456218402129964 3 0.0041085026195372505 4 9.537595366782902E-4 5 8.070273002662456E-4 6 7.336611820602232E-5 7 0.0 8 0.0 9 0.0018341529551505584 10-11 3.6683059103011163E-4 12-13 0.0011371748321933463 14-15 0.0027512294327258374 16-17 0.0024944480190047594 18-19 0.0 20-21 0.0 22-23 0.0018708360142535695 24-25 0.0010271256548843126 26-27 0.005392409688142641 28-29 0.006419535343026954 30-31 0.0012839070686053908 32-33 3.301475319271005E-4 34-35 0.009721010662297958 36-37 0.012875753745156919 38-39 0.010051158194225059 40-41 0.001980885191562603 42-43 5.135628274421563E-4 44-45 0.0011371748321933463 46-47 3.668305910301116E-5 48-49 7.336611820602233E-4 50-51 9.170764775752792E-4 52-53 0.002604497196313793 54-55 0.003374841437477027 56-57 0.0014306393050174354 58-59 0.0011371748321933463 60-61 0.0045120162696703735 62-63 0.0054290927472456525 64-65 0.009354180071267848 66-67 0.0031914261419619715 68-69 0.0033381583783740156 70-71 1.4673223641204465E-4 72-73 0.0015773715414294802 74-75 0.006309486165717921 76-77 0.0025311310781077705 78-79 0.003448207555683049 80-81 0.004878846860700485 82-83 0.005465775806348664 84-85 0.005502458865451675 86-87 0.0038884042649191836 88-89 0.009794376780503981 90-91 0.012142092563096696 92-93 0.0012105409503993685 94-95 0.0025311310781077705 96-97 0.0013939562459144243 98-99 0.003741672028507139 100-101 0.004585382387876396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1363027.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.18346405974832 #Duplication Level Percentage of deduplicated Percentage of total 1 72.02135742222066 33.98217129465637 2 14.232485265893652 13.430759140483813 3 5.380659307352075 7.616344351385908 4 2.5792265260441813 4.867873683742205 5 1.4461911573040611 3.4118154247090997 6 0.9279704684548912 2.62709167481095 7 0.5996727497721179 1.9806246345538223 8 0.4592959215002246 1.7336938083915867 9 0.33036223682758575 1.402887125524721 >10 1.802285795745061 15.421606358434836 >50 0.1431293636413911 4.609624514465403 >100 0.07113734438438735 6.171996313129841 >500 0.00466983064489753 1.5052076961512013 >1k 0.0015566102149658435 1.2383039795603228 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4574 0.3355766246743462 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1749 0.12831734074233306 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1548 0.11357075098292256 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1498 0.10990244507262145 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1396 0.10241910101560718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.668305910301117E-5 0.0 22-23 0.0 0.0 0.0 1.8341529551505582E-4 0.0 24-25 0.0 0.0 0.0 2.9346447282408935E-4 0.0 26-27 0.0 0.0 0.0 2.9346447282408935E-4 0.0 28-29 0.0 0.0 0.0 8.803934184722679E-4 0.0 30-31 0.0 0.0 0.0 0.0018341529551505584 0.0 32-33 0.0 0.0 0.0 0.0028979616691378824 0.0 34-35 0.0 0.0 0.0 0.0038150381467131613 0.0 36-37 0.0 0.0 0.0 0.005612508042760709 0.0 38-39 0.0 0.0 0.0 0.0074466609979112666 0.0 40-41 0.0 0.0 0.0 0.011811945031169595 0.0 42-43 0.0 0.0 0.0 0.017864649783166438 0.0 44-45 0.0 0.0 0.0 0.031033868001147446 0.0 46-47 0.0 0.0 0.0 0.06071046281548348 0.0 48-49 0.0 0.0 0.0 0.08187658791792092 0.0 50-51 0.0 0.0 0.0 0.10883863635863414 0.0 52-53 0.0 0.0 0.0 0.14196343872865322 0.0 54-55 0.0 0.0 0.0 0.17927010983641556 0.0 56-57 0.0 0.0 0.0 0.22097874803653927 0.0 58-59 0.0 0.0 0.0 0.28594444570797206 0.0 60-61 0.0 0.0 0.0 0.37024211552669173 0.0 62-63 0.0 0.0 0.0 0.47805362623044156 0.0 64-65 0.0 0.0 0.0 0.5559317607061343 0.0 66-67 0.0 0.0 0.0 0.6889812160727558 0.0 68-69 0.0 0.0 0.0 0.8549353754547782 0.0 70-71 0.0 0.0 0.0 0.9638473779316183 0.0 72-73 0.0 0.0 0.0 1.1616424326150545 0.0 74-75 0.0 0.0 0.0 1.3129600514149757 0.0 76-77 0.0 0.0 0.0 1.4383060643699648 0.0 78-79 0.0 0.0 0.0 1.6030863658606909 0.0 80-81 0.0 0.0 0.0 1.7687470607698894 0.0 82-83 0.0 0.0 0.0 1.9894690273927074 0.0 84-85 0.0 0.0 0.0 2.3454047498692248 0.0 86-87 0.0 0.0 0.0 2.801375174519654 0.0 88-89 0.0 0.0 0.0 3.3629194432685487 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8305 0.0 29.35179 1 TACATGG 8595 0.0 28.18731 2 ACATGGG 8530 0.0 27.11625 3 CGTATAG 150 1.0568856E-6 25.342855 1 CATGGGG 3805 0.0 23.71639 4 TAAGGTG 510 0.0 23.28196 5 CCTACAC 555 0.0 22.25 3 CATGGGA 4415 0.0 21.730532 4 CATGGGT 1225 0.0 20.54894 4 GTAAGGT 470 0.0 20.210722 4 GTATTAC 170 7.932297E-5 19.566175 1 ATGGGAG 1995 0.0 19.521833 5 GTGCATA 840 0.0 19.233418 1 GGGTACC 635 0.0 18.698896 7 GTATAGG 435 0.0 18.570196 1 ATACGTC 205 1.5911333E-5 18.53471 5 ATAGTAC 310 5.5915734E-9 18.385237 3 ATGGGAT 1505 0.0 18.303795 5 TACCGGA 130 0.0075602424 18.270733 2 GAGTACT 2120 0.0 18.147415 12-13 >>END_MODULE